Carbon dioxide incubator for cell culture

Abstract

The invention belongs to the technical field of cell culture, and particularly relates to a carbon dioxide incubator for cell culture, which comprises an incubator main body, an incubator door and a glass door are rotatably mounted on the incubator main body, the glass door is located between the incubator main body and the incubator door, an inner box is mounted in the incubator main body, and the incubator main body is provided with an outer box. A placing mechanism and a taking and placing mechanism are installed on the inner walls of the two sides of the inner box, carrying mechanisms which are arranged at equal intervals in a stacked mode are detachably installed on the placing mechanism, and circulating placing assemblies are detachably installed on the carrying mechanisms. The inside and outside of the inner box are separated through the glass door and the transfer mechanism, and in the process of taking out a culture dish containing a culture medium and when the detected culture dish is placed into the inner box again, through cooperation of the glass door and the transfer mechanism, the situation that the inner space and the outer space of the inner box are communicated is avoided, and the detection accuracy is improved. The culture medium in the culture dish which is always positioned in the inner box is not influenced by the external environment.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, in particular to a carbon dioxide incubator for cell culture. Background Art

[0002] Carbon dioxide incubators are widely used in cell and tissue culture and the cultivation of certain special microorganisms. They are commonly used in cell dynamics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, culture of hybridoma cells to produce antibodies, in vitro fertilization (IVF), stem cells, tissue engineering, drug screening and other research fields.

[0003] When observing the number of cells in the initial culture medium, the culture personnel are usually required to take the culture medium out of the incubator and then place it at the bottom of a microscope for observation. The operation is rather cumbersome, and the initial culture medium needs to be cultured in a sterile environment. During the process of taking out and putting in the culture medium, it is inevitable that some miscellaneous bacteria will enter the culture medium, which is inconvenient for the normal culture of immune cells. For this reason, a carbon dioxide incubator for cell culture is proposed. Summary of the Invention

[0004] In order to solve the shortcomings of the prior art, the present invention provides a carbon dioxide incubator for cell culture.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution: a carbon dioxide incubator for cell culture, comprising an incubator body, a door and a glass door rotatably mounted on the incubator body, the glass door being located between the incubator body and the door, an inner box being mounted in the incubator body, a placement mechanism and a pick-and-place mechanism being mounted on the inner walls on both sides of the inner box, a supporting mechanism being detachably mounted on the placement mechanism and a circulating placement assembly being detachably mounted on the supporting mechanism, culture dishes being transported in a ring shape on the circulating placement assembly, a transfer mechanism being mounted on the side of the glass door away from the incubator body, the pick-and-place mechanism being able to push culture dishes pushed out from the circulating placement assemblies at different heights into the transfer mechanism after adjusting the height.

[0006] Preferably, the circulation placement component includes a circulation placement frame, guide rollers are evenly laid on the bottom inner wall of the incubator body, a positioning port is provided on the circulation placement frame, an electric push rod is fixedly installed on the circulation placement frame, and two flexible clamps are fixedly installed on the piston rod end of the electric push rod, and the supporting mechanism includes two support plates, and the same frame is detachably installed on the two support plates, and a positioning block adapted to the positioning port is fixedly installed on the top of the frame.

[0007] Preferably, the circulation placement frame includes a horizontal frame and two vertical frames 2, and the ends of the two vertical frames 2 close to the horizontal frame are fixedly connected to the horizontal frame through a right-angle frame, and an intermediate frame is fixedly installed between the two reversing frames 2. The number of the intermediate frames is greater than or equal to one, and the intermediate frame includes a reversing frame 1 and two vertical frames 1, and the ends of the two vertical frames 1 close to the horizontal frame are fixedly connected to the reversing frame 1. The vertical frames 2 and the vertical frames 1 on both sides are connected through the same reversing frame 2. When the number of intermediate frames is more than one, the two adjacent vertical frames 1 on the two adjacent intermediate frames are connected through the same reversing frame 2 on the side away from the circulation placement frame, and a take-and-place opening 1 is provided at the center of the side of the circulation placement frame away from the intermediate frame.

[0008] Preferably, latches are fixedly installed on both sides of the support plate, and the placement mechanism includes four brackets fixedly installed on the inner walls on both sides of the inner box. Two brackets are fixedly installed on the inner wall of each side of the inner box, and a plurality of placement openings distributed linearly are opened on the brackets. The four latches on the two support plates are respectively inserted into the corresponding placement openings on the same horizontal plane.

[0009] Preferably, the picking and placing mechanism includes two linear modules fixedly mounted on the inner walls on both sides of the inner box, the same horizontal plate is fixedly mounted on the sliders of the two linear modules, a carrier plate is fixedly mounted on the top of the horizontal plate, two limit plates are fixedly mounted on the carrier plate, and the two limit plates are arranged in an outward-facing "X" shape on the side close to the placement mechanism.

[0010] Preferably, the picking and placing mechanism also includes a connecting frame fixedly mounted on the inner wall of the top of the inner box, the bottom of the connecting frame is fixedly mounted with an electric push rod 2, and the piston rod end of the electric push rod 2 is fixedly mounted with two-claw flexible clamp 2.

[0011] Preferably, a accommodating box for accommodating a transfer mechanism is fixedly installed on the box door, a through opening is provided on the glass door, and the transfer mechanism includes a transfer box fixedly installed on the side of the glass door away from the incubator body, a rectangular opening connected to the through opening is provided on the side of the transfer box close to the glass door, and a second take-and-place opening is provided on the top inner wall of the rectangular opening, a U-shaped plate is slidably installed in the transfer box, round rods are fixedly installed on both sides of the U-shaped plate, and the two round rods pass through the transfer box and are connected to the transfer box in a damping sliding manner, and a plug plate is detachably installed in the second take-and-place opening.

[0012] Preferably, a vent hole is provided on the inner wall of the inner box on the side away from the glass door, and a fan is fixedly installed on the inner wall of the inner box on the side away from the glass door.

[0013] Compared with the prior art, the present invention has the following beneficial effects: The present invention separates the inside and outside of the inner box by using a glass door and a transfer mechanism. When taking out a culture dish containing culture medium or when placing the culture dish back into the inner box after testing, the glass door and the transfer mechanism cooperate to prevent the inner and outer spaces of the inner box from being connected, thereby ensuring that the culture medium in the culture dish that is always in the inner box is not affected by the external environment. The specially shaped circulation placement frame can hold more culture dishes in the same area, and can cooperate with multiple guide rollers to ensure that each culture dish can move cyclically along a closed path, so that the position between two adjacent culture dishes will not change. At the same time, it can ensure that the specified culture dish on the circulation placement frame can be taken out through the cooperation of the pick-and-place mechanism, the electric push rod and the two-claw flexible clamp. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the overall structure of a carbon dioxide incubator for cell culture proposed by the present invention; Figure 2 This is an exploded view of a carbon dioxide incubator for cell culture proposed by the present invention; Figure 3 This is a partial structural schematic diagram of a carbon dioxide incubator for cell culture proposed by the present invention; Figure 4 This is a schematic structural diagram of a placement mechanism and a supporting mechanism in a carbon dioxide incubator for cell culture proposed by the present invention; Figure 5 This is a schematic structural diagram of a circulating placement component and culture dish in a carbon dioxide incubator for cell culture proposed by the present invention; Figure 6 This is a schematic structural diagram of a pick-and-place mechanism in a carbon dioxide incubator for cell culture proposed by the present invention; Figure 7 An exploded view of a transfer mechanism in a carbon dioxide incubator for cell culture proposed by the present invention and a partial structural diagram of a glass door; Figure 8 A top view of a circulating placement frame in a carbon dioxide incubator for cell culture proposed by the present invention and a schematic diagram of the movement path of a culture dish on the circulating placement frame; Figure 9 This is an exploded top view of a circulating placement frame in a carbon dioxide incubator for cell culture proposed by the present invention; Figure 10 This is a top view of another form of a circulating placement frame in a carbon dioxide incubator for cell culture proposed by the present invention.

[0015] In the figure: 1. Incubator body; 2. Door; 21. Storage box; 3. Glass door; 31. Passageway; 4. Inner box; 41. Ventilation hole; 42. Fan; 5. Placement mechanism; 51. Bracket; 52. Placement opening; 6. Support mechanism; 61. Support plate; 62. Latch; 63. Positioning block; 64. Frame; 7. Circular placement assembly; 71. Circular placement frame; 711. Horizontal frame; 712. Middle frame; 7121. Vertical frame 1; 7122. Reversing frame 1; 713. Reversing frame 2; 71 4. Second vertical frame; 715. Right-angle frame; 72. Positioning port; 73. Guide roller; 74. First pick-up and placement port; 75. First electric push rod; 76. First two-claw flexible clamp; 8. Pick-up and placement mechanism; 81. Linear module; 82. Horizontal plate; 83. Carrying plate; 84. Limiting plate; 85. Second electric push rod; 86. Second two-claw flexible clamp; 87. Connecting frame; 9. Transfer mechanism; 91. Transfer box; 92. Rectangular port; 93. Second pick-up and placement port; 94. U-shaped plate; 95. Round rod; 96. Plug plate; 10. Culture dish. DETAILED DESCRIPTION

[0016] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0017] Please refer to Figures 1-10 The present invention provides a technical solution: a carbon dioxide incubator for cell culture, comprising an incubator body 1, a door 2 and a glass door 3 are rotatably mounted on the incubator body 1, the glass door 3 is located between the incubator body 1 and the door 2, an inner box 4 is mounted in the incubator body 1, a placement mechanism 5 and a pick-and-place mechanism 8 are mounted on the inner walls of both sides of the inner box 4, the placement mechanism 5 is detachably mounted with supporting mechanisms 6 stacked at equal intervals, the supporting mechanism 6 is detachably mounted with a circulating placement component 7, the circulating placement component 7 transports culture dishes 10 in a ring shape, a transfer mechanism 9 is mounted on the side of the glass door 3 away from the incubator body 1, and the pick-and-place mechanism 8 can push the culture dishes 10 pushed out from the circulating placement components 7 at different heights into the transfer mechanism 9 after adjusting the height.

[0018] Furthermore, the humidity inside the inner box 4 can be adjusted by a humidifying device on the inner wall of the bottom of the inner box 4 .

[0019] The circulation placement component 7 includes a circulation placement frame 71, guide rollers 73 are evenly laid on the bottom inner wall of the incubator body 1, a positioning port 72 is provided on the circulation placement frame 71, an electric push rod 75 is fixedly installed on the circulation placement frame 71, and two flexible clamps 76 are fixedly installed on the piston rod end of the electric push rod 75. The supporting mechanism 6 includes two supporting plates 61, and the same frame 64 is detachably installed on the two supporting plates 61. A positioning block 63 adapted to the positioning port 72 is fixedly installed on the top of the frame 64.

[0020] Furthermore, the multiple guide rollers 73 on the same circulation placement frame 71 are driven by the same driving device.

[0021] The circulation placement frame 71 includes a horizontal frame 711 and two vertical frames 714. The ends of the two vertical frames 714 close to the horizontal frame 711 are fixedly connected to the horizontal frame 711 through a right-angle frame 715. An intermediate frame 712 is fixedly installed between the two reversing frames 713. The number of intermediate frames 712 is greater than or equal to one. The intermediate frame 712 includes a reversing frame 1 7122 and two vertical frames 1 7121. The ends of the two vertical frames 1 7121 close to the horizontal frame 711 are fixedly connected to the reversing frame 1 7122. The vertical frames 2 714 and the vertical frames 1 7121 on both sides are connected by the same reversing frame 2 713. When the number of intermediate frames 712 is more than one, the two adjacent vertical frames 1 7121 on the two adjacent intermediate frames 712 are connected by the same reversing frame 2 713 on the side away from the circulation placement frame 71. A take-and-place opening 74 is provided at the center of the side of the circulation placement frame 71 away from the intermediate frame 712.

[0022] Furthermore, the second reversing frame 713 and the first reversing frame 7122 cooperate with the guide rollers 73 thereon to transport the culture dish 10 in the opposite direction of the incoming direction, while the right-angle frame 715 cooperates with the guide rollers 73 thereon to transport the culture dish 10 in a direction perpendicular to the incoming direction; like Figures 8-10 As shown, according to the different sizes of the inner box 4 and the frame 64, the horizontal frame 711, the vertical frame 2 714 and the reversing frame 1 7122 of different lengths can be used to adjust the longitudinal width of the circular placement frame 71; By using different numbers of the middle frames 712 and the second reversing frames 713 and using transverse frames 711 of different lengths, the transverse length of the circular placement frame 71 can be adjusted.

[0023] There are latches 62 fixedly installed on both sides of the support plate 61. The placement mechanism 5 includes four brackets 51 fixedly installed on the inner walls of both sides of the inner box 4. Two brackets 51 are fixedly installed on each inner wall of the inner box 4. The brackets 51 are provided with multiple placement openings 52 distributed linearly. The four latches 62 on the two support plates 61 are respectively inserted into the corresponding placement openings 52 on the same horizontal plane.

[0024] The pick-and-place mechanism 8 includes two linear modules 81 fixedly mounted on the inner walls on both sides of the inner box 4. The same horizontal plate 82 is fixedly mounted on the sliders of the two linear modules 81. A carrier plate 83 is fixedly mounted on the top of the horizontal plate 82. Two limit plates 84 are fixedly mounted on the carrier plate 83. The two limit plates 84 are arranged in an outward-facing "X" shape on the side close to the placement mechanism 5.

[0025] Furthermore, since the two limiting plates 84 are arranged in an outward-facing "X" shape on one side close to the placement mechanism 5 , it is convenient to push the culture dish 10 from the placement port 1 74 to between the two limiting plates 84 .

[0026] The pick-and-place mechanism 8 also includes a connecting frame 87 fixedly mounted on the top inner wall of the inner box 4 , a second electric push rod 85 fixedly mounted on the bottom of the connecting frame 87 , and two flexible clamps 86 fixedly mounted on the piston rod end of the second electric push rod 85 .

[0027] Furthermore, the two-claw flexible clamp 1 76 and the two-claw flexible clamp 2 86 only need to clamp a part of the side surface of the culture dish 10 to drive the culture dish 10 to move.

[0028] A accommodating box 21 for accommodating the transfer mechanism 9 is fixedly installed on the box door 2, and a through port 31 is provided on the glass door 3. The transfer mechanism 9 includes a transfer box 91 fixedly installed on the side of the glass door 3 away from the incubator body 1. A rectangular opening 92 connected to the through port 31 is provided on the side of the transfer box 91 close to the glass door 3, and a second take-and-place opening 93 is provided on the top inner wall of the rectangular opening 92. A U-shaped plate 94 is slidably installed in the transfer box 91, and round rods 95 are fixedly installed on both sides of the U-shaped plate 94. The two round rods 95 pass through the transfer box 91 and are connected to the transfer box 91 in a damped sliding manner. A plug plate 96 is detachably installed in the door of the second take-and-place opening 93.

[0029] A vent hole 41 is formed on the inner wall of the inner box 4 at a side away from the glass door 3 , and a fan 42 is fixedly mounted on the inner wall of the inner box 4 at a side away from the glass door 3 .

[0030] In this embodiment, when in use, a culture dish 10 containing culture medium can be placed in the circulation placement frame 71. Multiple culture dishes 10 can be placed in the circulation placement frame 71, and each culture dish 10 can be numbered. When culturing the culture medium, the box door 2 and the glass door 3 are closed, so that the inner box 4 is in a closed state. By starting the fan 42 to send air into the inner box 4 through the vent 41, the carbon dioxide concentration in the inner box 4 is made more uniform. When it is necessary to take out the designated numbered culture dish 10 on the target circulation placement component 7, the box door 2 is opened and the glass door 3 is in a closed state. At this time, the inside and outside of the inner box 4 are not connected due to the obstruction of the glass door 3. The two linear modules 81 drive the horizontal plate 82 to adjust the height of the carrier plate 83 to be flush with the bottom inner wall of the target circulation placement frame 71. Then, the designated numbered culture dish 10 is transported to the take-and-put port 74 by the multiple guide rollers 73 on the circulation placement frame 71. Then, the electric push rod 75 on the circulation placement component 7 is controlled to extend so that it clamps the designated numbered culture dish 10 through the two-claw flexible clamp 76 and pushes the culture dish 10 out of the take-and-put port 74 and moves it onto the carrier plate 83. Then, the two-claw flexible clamp 76 releases the culture dish 10 and resets the electric push rod 75. Then, the two linear modules 81 are controlled to move the carrier 83 and the designated culture dish 10 thereon to the highest point. Then, the second electric push rod 85 is controlled to extend, so that the two-claw flexible clamp 86 clamps the designated culture dish 10 on the carrier 83 and pushes the culture dish 10 through the through port 31 and the rectangular port 92 into the transfer box 91 and into the U-shaped plate 94. Then, the two-claw flexible clamp 86 releases the culture dish 10 and the second electric push rod 85 is reset. Then, the two round rods 95 push and pull the U-shaped plate 94 to move the designated numbered culture dish 10 to the bottom of the stopper plate 96. After that, the stopper plate 96 is opened and the designated numbered culture dish 10 in the U-shaped plate 94 can be taken out from the second access opening 93. At this time, due to the obstruction of the U-shaped plate 94, the rectangular opening 92 will not be connected to the external environment through the second access opening 93 in the open state. Then, the culture medium in the taken designated numbered culture dish 10 can be tested. After the culture medium in the designated numbered culture dish 10 has been inspected, if it needs to be placed back to its original position, the culture dish 10 after inspection can be placed into the transfer box 91 through the opened access port 2 93 and made to fall into the U-shaped plate 94, and then the access port 2 93 is blocked by the plug plate 96, and then the U-shaped plate 94 is driven to move the culture dish 10 by pushing and pulling the round rod 95. When the inner walls on both sides of the opening of the U-shaped plate 94 are flush with the inner walls on both sides of the rectangular opening 92, the electric push rod 2 85 is controlled to extend, so that it drives the two-claw flexible clamp 2 86 to pass through the through port 31 and the rectangular opening 92 to clamp the culture dish 10 in the U-shaped plate 94, and then the electric push rod 2 85 is controlled to shorten. When the two-claw flexible clamp 2 86 drives the culture dish 10 to move onto the carrier plate 83, the two-claw flexible clamp 2 86 releases the culture dish 10 and the electric push rod 2 85 is reset. Then, by controlling the two linear modules 81, the horizontal plate 82 drives the culture dish 10 on the carrier plate 83 after inspection to move to the target height, so that the carrier plate 83 is flush with the inner wall of the bottom of the target height circulation placement frame 71, and then the electric push rod 75 on the circulation placement component 7 is controlled to extend, so that the electric push rod 75 drives the two-claw flexible clamp 76 to clamp the culture dish 10 on the carrier plate 83, and then the electric push rod 75 is controlled to shorten, and the two-claw flexible clamp 76 will drive the culture dish 10 to pass through the take-and-place port 74 and fall back on the circulation placement frame 71, and at the same time, the culture dish 10 can push away the two culture dishes 10 originally at the take-and-place port 74 on the circulation placement frame 71 and fall between the two culture dishes 10, so that the culture dish 10 can finally fall back to its original position after inspection.

[0031] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A carbon dioxide incubator for cell culture, comprising an incubator body (1), characterized in that: The incubator body (1) is rotatably mounted with a door (2) and a glass door (3), the glass door (3) being located between the incubator body (1) and the door (2), the incubator body (1) being mounted with an inner box (4), the inner walls of both sides of the inner box (4) being mounted with a placement mechanism (5) and a pick-up mechanism (8), the placement mechanism (5) being detachably mounted with a supporting mechanism (6) stacked at equal intervals, the supporting mechanism (6) being detachably mounted with a circulating placement component (7), the circulating placement component (7) being configured to transport culture dishes (10) in a ring shape, the glass door (3) being mounted with a transfer mechanism (9) on a side away from the incubator body (1), the pick-up mechanism (8) being configured to push culture dishes (10) pushed out from the circulating placement components (7) at different heights into the transfer mechanism (9) after adjusting the height.

2. A carbon dioxide incubator for cell culture according to claim 1, characterized in that: The circulation placement component (7) includes a circulation placement frame (71), a guide roller (73) is evenly laid on the bottom inner wall of the incubator body (1), a positioning hole (72) is provided on the circulation placement frame (71), an electric push rod (75) is fixedly installed on the circulation placement frame (71), and two flexible clamps (76) are fixedly installed at the end of the piston rod of the electric push rod (75), and the supporting mechanism (6) includes two supporting plates (61), and the same frame (64) is detachably installed on the two supporting plates (61), and a positioning block (63) adapted to the positioning hole (72) is fixedly installed on the top of the frame (64).

3. A carbon dioxide incubator for cell culture according to claim 2, characterized in that: The cycle placement frame (71) includes a horizontal frame (711) and two vertical frames (714). The ends of the two vertical frames (714) close to the horizontal frame (711) are fixedly connected to the horizontal frame (711) through a right-angle frame (715). An intermediate frame (712) is fixedly installed between the two reversing frames (713). The number of the intermediate frames (712) is greater than or equal to one. The intermediate frame (712) includes a reversing frame (7122) and two vertical frames (7121). The two vertical frames (7121) are close to the horizontal frame (71 1) are fixedly connected to the reversing frame 1 (7122), and the vertical frame 2 (714) and the vertical frame 1 (7121) on both sides are connected through the same reversing frame 2 (713). When the number of the intermediate frames (712) is more than one, the two adjacent vertical frames 1 (7121) on the two adjacent intermediate frames (712) are connected on the side away from the circulation placement frame (71) through the same reversing frame 2 (713), and the circulation placement frame (71) is provided with a take-out opening 1 (74) at the center of the side away from the intermediate frame (712).

4. The carbon dioxide incubator for cell culture according to claim 2, characterized in that: Both sides of the support plate (61) are fixedly mounted with latches (62). The placement mechanism (5) comprises four brackets (51) fixedly mounted on the inner walls of both sides of the inner box (4). Two brackets (51) are fixedly mounted on the inner wall of each side of the inner box (4). The brackets (51) are provided with a plurality of placement openings (52) distributed linearly. The four latches (62) on the two support plates (61) are respectively inserted into corresponding placement openings (52) on the same horizontal plane.

5. The carbon dioxide incubator for cell culture according to claim 1, characterized in that: The pick-and-place mechanism (8) comprises two linear modules (81) fixedly mounted on the inner walls of both sides of the inner box (4); a same horizontal plate (82) is fixedly mounted on the sliders of the two linear modules (81); a carrier plate (83) is fixedly mounted on the top of the horizontal plate (82); two limit plates (84) are fixedly mounted on the carrier plate (83); the two limit plates (84) are arranged in an outward-facing "X" shape on one side close to the placement mechanism (5).

6. The carbon dioxide incubator for cell culture according to claim 5, characterized in that: The pick-and-place mechanism (8) further comprises a connecting frame (87) fixedly mounted on the inner wall of the top of the inner box (4), a second electric push rod (85) fixedly mounted on the bottom of the connecting frame (87), and a second flexible clamping claw (86) with two claws fixedly mounted on the end of the piston rod of the second electric push rod (85).

7. The carbon dioxide incubator for cell culture according to claim 1, characterized in that: A accommodating box (21) for accommodating a transfer mechanism (9) is fixedly installed on the box door (2), a through opening (31) is provided on the glass door (3), and the transfer mechanism (9) includes a transfer box (91) fixedly installed on the side of the glass door (3) away from the incubator body (1), a rectangular opening (92) connected to the through opening (31) is provided on the side of the transfer box (91) close to the glass door (3), and a second access opening (93) is provided on the top inner wall of the rectangular opening (92), a U-shaped plate (94) is slidably installed in the transfer box (91), and round rods (95) are fixedly installed on both sides of the U-shaped plate (94), and the two round rods (95) both penetrate the transfer box (91) and are connected to the transfer box (91) in a damping sliding manner, and a plug plate (96) is detachably installed in the door of the second access opening (93).

8. The carbon dioxide incubator for cell culture according to claim 1, characterized in that: A vent hole (41) is provided on the inner wall of the inner box (4) on the side away from the glass door (3), and a fan (42) is fixedly mounted on the inner wall of the inner box (4) on the side away from the glass door (3).

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