TaqMan real-time fluorescent quantitative PCR probe and primer combination for identifying ampullaria gigas, kit and application of TaqMan real-time fluorescent quantitative PCR probe and primer combination
By designing the probe primer combination of TaqMan real-time fluorescence quantitative PCR in Fushou Snail, the problem of insufficient specificity and sensitivity of Fushou Snail detection in the existing technology has been solved, and the rapid and accurate Fushou Snail identification has been achieved, which has promoted the progress of related prevention and control and research work.
CN120442820APending Publication Date: 2025-08-08KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Patent Information
- Application Number
- CN202510818043.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-18
- Publication Date
- 2025-08-08
AI Technical Summary
Technical Problem
The existing Fushou snail detection methods are not specific, prone to non-specific amplification, and insufficient detection sensitivity and reliability, making it difficult to achieve rapid and accurate identification.
Method used
A probe primer combination for real-time fluorescence quantitative PCR of Fushou Snail was designed, including specific probes, upstream primers and downstream primers. Through real-time fluorescence quantitative PCR reaction of TaqMan, combined with specific amplification conditions, high specificity and sensitivity detection of Fushou Snail was achieved.
Benefits of technology
It improves the accuracy and sensitivity of Fushou snail detection, shortens the detection time, achieves fast, stable and reliable identification, and reduces costs.
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Abstract
The embodiment of the invention discloses a probe primer combination for identifying TaqMan real-time fluorescent quantitative PCR of ampullaria gigas, a kit and application. Comprising a probe, an upstream primer and a downstream primer, the nucleotide sequence of the probe is 5 '-FAM-CCTTCTCTATTACTACTATTA-MGB-3', the nucleotide sequence of the upstream primer is 5 '-CTTTCCGCGTCTAATAACATG-3', and the nucleotide sequence of the downstream primer is 5 '-CATCAGTTCCAGCACCACTC-3'. The TaqMan probe real-time fluorescent PCR probe and primer combination established by the invention has excellent specificity and sensitivity in the aspect of identifying the species of the ampullaria gigas, the detection accuracy is greatly improved, the detection time is shortened, the effects of stability, reliability, short period and low cost are achieved, and the primer combination has good application prospects in rapid and accurate identification of the ampullaria gigas. The method is of great significance in promoting related prevention and research work.
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