Cream containing rumex mairei fruit oil ceramide as well as preparation method and application of cream containing rumex mairei fruit oil ceramide

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to cream containing rosa palmifolia fruit oil ceramide as well as a preparation method and application of the cream. Specifically, the cream is prepared by taking the rosa palmifolia fruit oil ceramide, the batyl alcohol and the ruscogenin as main components, and tests prove that the prepared cream containing the rosa palmifolia fruit oil ceramide, the batyl alcohol and the ruscogenin composition can remarkably promote the generation of barrier related protein FLG, promote the generation of laminin, inhibit the generation of the linker, inhibit the generation of the linker, inhibit the generation of the linker, and inhibit the generation of the linker and the linker, so that the rosa palmifolia fruit oil ceramide, the batyl alcohol and the ruscogenin can be used for preparing the cream. The ceramide cream has the effects of repairing skin barrier and resisting aging of a true epidermis connecting layer, can remarkably improve the problem that the high-temperature and low-temperature emulsification particle size of cream containing high-content ceramide becomes large, and is mild and non-irritant, so that the ceramide cream has good practical application value.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a cream containing palmetto fruit oil ceramide, a preparation method and an application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Skin aging is a complex biological process, influenced by both internal factors like natural aging and external factors like the environment and lifestyle. This process manifests as a decline in skin barrier function, elastic fiber breakdown, reduced laminin content, and accelerated epidermal water loss, exacerbating aging symptoms like wrinkles, dryness, and sensitivity. Currently, anti-aging products often incorporate antioxidants like vitamin C, resveratrol, peptides, and retinols. However, these products suffer from a single pathway of action and struggle to simultaneously address the synergistic effects of barrier damage and aging. Furthermore, some ingredients, like vitamin C and retinol, are irritants to the skin, easily causing adverse reactions like erythema and scaling, making them unsuitable for sensitive skin.

[0004] In recent years, ceramides have become a research hotspot in the anti-aging field due to their dual restorative properties: strengthening the skin barrier and restoring skin moisture. Natural plant-derived ceramides have a similar structure to the lipids in the human stratum corneum and offer the advantage of being environmentally friendly and sustainable. However, the inventors have discovered that existing technologies still face the following bottlenecks: Plant ceramides alone have limited efficacy, low bioavailability, and difficulty addressing multi-dimensional aging repair mechanisms. Furthermore, in traditional cream systems, high ceramide content can easily cause the cream's emulsified particles to increase in size under high and low temperature conditions, leading to problems such as discontinuous application and a rough texture. Summary of the Invention

[0005] In view of the deficiencies in the prior art, the present invention aims to provide a cream containing palm oil ceramide, a preparation method, and an application thereof. Specifically, the present invention uses palm oil ceramide, batyl alcohol, and ruscosapogenin as main ingredients in a cream. Experimental verification shows that the cream containing the palm oil ceramide, batyl alcohol, and ruscosapogenin composition can significantly promote the generation of barrier-related protein FLG and laminin, thereby repairing the skin barrier and providing anti-aging effects on the epidermal junction layer. At the same time, the problem of large particle size caused by high-low temperature emulsification of creams containing high ceramide content can be significantly improved, and the cream is gentle and non-irritating. Based on the above research results, the present invention has been completed.

[0006] In order to achieve the above technical objectives, the technical solutions provided by the present invention are as follows:

[0007] In a first aspect of the present invention, a cream containing ceramide from tiliaceae fruit oil is provided, wherein the cream comprises at least the following components in parts by weight:

[0008] 0.1-5 parts of palm oil ceramide, 0.05-0.5 parts of batyl alcohol, and 0.01-0.1 parts of ruscosapogenin.

[0009] Furthermore, the components include: 1.5-3 parts of palm oil ceramide, 0.2-0.4 parts of batyl alcohol, and 0.05-0.08 parts of ruscosapogenin.

[0010] Furthermore, the cream also contains cosmetic excipient ingredients commonly used in cream preparations, such as emulsifiers, oils, polyols, thickeners, preservatives, chelating agents and solvents.

[0011] The ceramide from the palm oil is obtained by reacting the fatty acids from the palm oil with sphingosine compounds.

[0012] A second aspect of the present invention provides a method for preparing the above-mentioned cream containing palmetto fruit oil ceramide, the preparation method comprising:

[0013] S1. Preparation of aqueous phase: Mix polyol, thickener, chelating agent, aqueous emulsifier and water, heat to 75-80°C and stir to dissolve;

[0014] S2. Preparation of oil phase: Heat the oil phase emulsifier and oil to 70-75°C to melt. After complete melting, add palm oil ceramide and batyl alcohol before mixing, and stir until uniformly melted.

[0015] S3, homogenizing the aqueous phase and the oil phase; adding ruscosapogenin after cooling; further, ruscosapogenin needs to be heated to 85° C. and stirred to dissolve with butanediol in advance;

[0016] S4: Cool down to 65-70℃, add preservative, stir to dissolve, then stir and cool down to about 38℃.

[0017] The third aspect of the present invention provides use of the cream containing palmetto fruit oil ceramide in the preparation of cosmetics.

[0018] A fourth aspect of the present invention provides a cosmetic comprising at least the above-mentioned cream containing palmetto fruit oil ceramide. The cosmetic is a skin care product.

[0019] Beneficial technical effects of one or more of the above technical solutions:

[0020] (1) In the stability study of the cream system containing high content of phytoceramide, there is a problem that the emulsified particle size becomes larger under high temperature and hot and cold cycle conditions. The combination of palm oil ceramide, batyl alcohol and ruscosapogenin can synergistically improve the problem of the emulsified particle size becoming larger in the cream containing high content of phytoceramide, and greatly improve the stability of the cream.

[0021] (2) In vitro tests have shown that a cream containing a combination of ceramides from palm oil, batyl alcohol, and ruscosaponin can achieve the effect of repairing and anti-aging by synergistically promoting the production of barrier-related proteins FLG and laminin, and the synergistic effect of a cream containing a combination of ceramides from palm oil, batyl alcohol, and ruscosaponin is better than that of a cream containing a combination of ceramides from sea buckthorn oil, batyl alcohol, and ruscosaponin.

[0022] (3) Human trials have shown that the cream prepared by this invention can significantly reduce the TEWL value, improve skin elasticity R2 and R7, and has good human body repair and anti-aging effects.

[0023] (4) The preparation method of the cream containing the composition of palm oil ceramide, batyl alcohol and ruscosaponin prepared by the above technical solution is simple and easy, saves costs, and the raw materials are easy to obtain, which is suitable for mass production. It has passed the human efficacy experiment test and has significant anti-aging and repair effects, so it has good practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The accompanying drawings, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0025] Figure 1 : Microstructure of Example 3 of the present invention after three months of observation at 45°C;

[0026] Figure 2 : Microstructure diagram of comparative example 3 of the present invention after being examined at 45°C for three months. DETAILED DESCRIPTION

[0027] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present invention belongs.

[0028] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0029] In a typical embodiment of the present invention, a cream containing ceramide from safflower oil is provided, wherein the cream comprises at least the following components in parts by weight:

[0030] 0.1-5 parts of palm oil ceramide, 0.05-0.5 parts of batyl alcohol, and 0.01-0.1 parts of ruscosapogenin.

[0031] Furthermore, the components include: 1.5-3 parts of palm oil ceramide, 0.2-0.4 parts of batyl alcohol, and 0.05-0.08 parts of ruscosapogenin.

[0032] Furthermore, the cream also contains cosmetic auxiliary ingredients commonly used in cream preparations, such as emulsifiers (further such as hydrogenated lecithin, arachidyl alcohol / behenyl alcohol / arachidyl glucoside), oils (further such as dicaprylyl carbonate, isononyl isononanoate, polydimethylsiloxane, caprylic / capric triglyceride, behenyl alcohol, cetearyl alcohol), polyols (further such as glycerol, dipropylene glycol, butylene glycol), thickeners (further such as sodium polyacrylate, ammonium acryloyldimethyltaurate / VP copolymer), preservatives (further such as 1,2-hexanediol, p-hydroxyacetophenone), chelating agents (further such as disodium EDTA) and solvents (such as water), etc., which are not specifically limited here.

[0033] The ceramides of the ...

[0034] Specifically, the cream contains at least the following components in parts by mass:

[0035] 0.1-5 parts of palm oil ceramide, 0.05-0.5 parts of batyl alcohol, 0.01-0.1 parts of ruscosapogenin, 0.3-5.0 parts of emulsifier, 5.0-20.0 parts of oil, 2.0-10.0 parts of polyol, 0.1-1.0 parts of thickener, 0.3-1.5 parts of preservative, 0.05-0.20 parts of chelating agent, and 90-100 parts of water.

[0036] Preferably, the cream contains at least the following components in parts by mass:

[0037] 1.5-3 parts of palm oil ceramide, 0.2-0.4 parts of batyl alcohol, 0.05-0.08 parts of ruscosapogenin, 2.0-5.0 parts of emulsifier, 6.0-12.0 parts of oil, 3.0-8.0 parts of polyol, 0.5-1.0 parts of thickener, 0.3-0.8 parts of preservative, 0.05-0.15 parts of chelating agent, and 93-98 parts of water.

[0038] In another embodiment of the present invention, a method for preparing the cream containing palmyra palm fruit oil ceramide is provided, the preparation method comprising:

[0039] S1. Preparation of aqueous phase: Mix polyol, thickener, chelating agent, aqueous emulsifier and water, heat to 75-80°C and stir to dissolve;

[0040] S2. Preparation of oil phase: Heat the oil phase emulsifier and oil to 70-75°C to melt. After complete melting, add palm oil ceramide and batyl alcohol before mixing, and stir until uniformly melted.

[0041] S3. The aqueous phase and the oil phase are combined and homogenized; when the temperature drops to about 70° C., ruscosapogenin is added; further, the ruscosapogenin needs to be heated to 85° C. with butanediol in advance and stirred to dissolve before being added.

[0042] S4: Cool down to 65-70℃, add preservative, stir to dissolve, then stir and cool down to about 38℃.

[0043] In another specific embodiment of the present invention, there is provided use of the cream containing palmyra palm fruit oil ceramide in the preparation of cosmetics.

[0044] In another embodiment of the present invention, a cosmetic is provided, comprising at least the aforementioned cream containing palmitoyl palmitate fruit oil ceramide. The cosmetic is a skin care product. Experiments have demonstrated that the cosmetic produced by the present invention can significantly improve the problem of increased emulsified particle size in creams. Furthermore, it can significantly promote the production of barrier-related proteins (FLG) and laminin, a protein associated with the dermal epidermal junction layer. Furthermore, it can significantly reduce TEWL (Texas-likely Wrinkle-Liver) values and improve skin elasticity (R2 and R7), demonstrating significant repair and anti-aging benefits while remaining mild and non-irritating.

[0045] The present invention will be further described below in conjunction with the examples. The present invention will be further described below by way of examples, but the present invention is not limited to the scope of the examples. Based on the examples in the present invention, any variation of the present invention by those skilled in the art without making any creative premise falls within the scope of protection of the present invention. Meanwhile, in the examples of the present invention, unless otherwise specified, all preparation raw materials are commercially available products well known to those skilled in the art.

[0046] In the examples and comparative examples of the present invention, the palmyra palm fruit oil ceramide was prepared by the following method:

[0047] S1: 50 g of rue dahliae fruit oil was mixed with 100 ml of ethanol and stirred to dissolve. 80 ml of a 20 wt % potassium hydroxide solution was slowly added dropwise at room temperature. After the addition was complete, the temperature was raised to 65° C. for reaction and monitored by TLC until saponification was complete. After saponification was complete, 3 mol / L dilute hydrochloric acid was added to adjust the pH to 3. The aqueous phase was extracted with 150 ml of ethyl acetate three times. The aqueous extracts were combined and washed once with 50 ml of saturated brine. The organic phase was dried over anhydrous Na2SO4 (1 g / 10 ml organic phase), filtered, and concentrated in vacuo to obtain 44 g of rue dahliae fruit oil fatty acids.

[0048] S2: 50 mmol of phytosphingosine fatty acids (calculated based on the main fatty acid component), 78 mmol of HOBt (hydroxybenzotriazole), 65 mmol of EDCI (1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride), and 65 mmol of DIPEA (N,N-diisopropylethylamine) were added to a 250 mL round-bottom flask. 100 mL of dichloromethane and 25 mL of dimethylformamide (DMF) were then added. The mixture was stirred at room temperature for 1.5 hours. 55 mmol of phytosphingosine was then added to the reaction system and stirred at room temperature until the reaction was complete as determined by TLC. After the reaction was complete, water was added to quench the reaction. The organic layer was separated, dried, filtered, and concentrated in vacuo, followed by washing with a solvent to obtain ceramide from ceramide from ceramide. Purification by gradient column chromatography was performed to retain only the fraction containing ceramide, which was concentrated to dryness at 45°C to yield 15.4 g of ceramide from ceramide from ceramide from ceramide.

[0049] S3: HPLC analysis showed: oleic acid-phytosphingosine ceramide 67%; linoleic acid-phytosphingosine ceramide 15.5%; palmitic acid-phytosphingosine ceramide 10%; stearic acid-phytosphingosine ceramide 2.5%; linolenic acid-phytosphingosine ceramide 0.3%.

[0050] Examples 1-4 and Comparative Examples 1-8:

[0051] The specific scheme is shown in Table 1 below, calculated by mass fraction.

[0052] Table 1 Specific Examples and Comparative Examples Ingredients

[0053]

[0054]

[0055] Note: The seabuckthorn oil ceramide in the comparative example was purchased from Shenzhen Dickman Biotechnology Co., Ltd.

[0056] Preparation method:

[0057] S1: Preparation of aqueous phase: Add the raw materials in phase A separately, heat to 80℃ and stir to dissolve;

[0058] S2: Preparation of oil phase: Add the raw materials of phase B except ceramides of palm fruit oil (ceramides of sea buckthorn oil), batyl alcohol, and ceramides of sea buckthorn oil separately, heat to 75°C and melt until they are completely dissolved. Before the ceramides of palm fruit oil (ceramides of sea buckthorn oil), batyl alcohol, and ceramides of sea buckthorn oil are combined, add phase B and stir until they are evenly melted.

[0059] S3: Combine phases A and B and homogenize at 3000 rpm for 12 minutes. After homogenization, cool the mixture. S4: When the temperature drops to 70°C, add phase C. Ruscogenin should be preheated to 85°C with butanediol and stirred to dissolve. Then, add the mixture directly to the 70°C combined phase liquid, stir evenly, and continue cooling.

[0060] S5: When the temperature drops to 65°C, add phase D and stir to dissolve. After dissolution, continue to cool.

[0061] S6: Stop stirring when the temperature drops to 38°C.

[0062] Test Example 1: Stability Test

[0063] The samples of Examples 1-4 and Comparative Examples 1-8 were subjected to stability observation at -18°C, 4°C, and 45°C, respectively. Microscopic observations were performed once a week at -18°C, 4°C, and 45°C for a period of 12 weeks to check whether the emulsified particle size showed a significant trend of increasing. The scoring criteria were: no particle size increase within the 12-week observation period was scored as 13; particle size increase within the 10-12 week observation period was scored as 10-12; particle size increase within the 7-9 week observation period was scored as 7-9; particle size increase within the 4-6 week observation period was scored as 4-6; and particle size increase within the 3-week observation period was scored as 1-3. The results are shown in Table 2.

[0064] Table 2 Stability test results

[0065] -18℃ 4℃ 45℃ Average score Example 1 13 13 13 13.0 Example 2 13 13 13 13.0 Example 3 13 13 13 13.0 Example 4 13 13 13 13.0 Comparative Example 1 8 13 3 8.0 Comparative Example 2 10 13 10 11.0 Comparative Example 3 10 13 6 9.7 Comparative Example 4 9 13 5 9.0 Comparative Example 5 8 13 4 8.3 Comparative Example 6 10 13 8 10.3 Comparative Example 7 10 13 8 10.7 Comparative Example 8 10 13 7 10.0

[0066] From Table 2, Figure 1 、 Figure 2 It can be seen that Examples 1-4 have good stability, and Comparative Examples 1-8 all have a tendency of increasing emulsified particle size to varying degrees, indicating that ceramides from palm fruit oil, batyl alcohol, and ruscoside can synergistically improve the problem of increased particle size during high and low temperature emulsification of the cream, thereby avoiding unstable factors such as discontinuous application of the cream and rough texture caused by increased particle size.

[0067] Test Example 2: Irritation Evaluation Test

[0068] The experiment recruited 30 subjects (2 males and 28 females), aged 18 to 60 years old.

[0069] Human skin occlusive patch test method: Dilute the test samples of Examples 1-4 with distilled water to a 1% solution as the test substance, and distilled water as the control group. Select a qualified patch tester and place 0.020-0.025 mL of the test substance into the patch tester chamber. Apply the patch tester containing the test substance to the flexed forearm of the subject for 24 hours. After 24 hours, remove the test substance patch tester, and observe the skin reaction 0.5 hours, 24 hours, and 48 hours after removal. Record the results according to the skin reaction grading standard in the "Cosmetic Safety Technical Specifications 2015" (Table 4). Detailed results are shown in Table 3. According to the interpretation of results in the "Cosmetic Hygiene Standards (2007 Edition)", the interpretation standard for the skin occlusive patch test results is: among 30 subjects, if more than 5 cases experience a Grade 1 adverse skin reaction, more than 2 cases experience a Grade 2 adverse skin reaction, or any case of a Grade 3 or higher adverse skin reaction occurs, the test substance is considered to have an adverse skin reaction on humans.

[0070] Table 3 Skin reaction grading standards

[0071]

[0072] The test results are shown in Table 4. No adverse reactions occurred in Examples 1-4, indicating that the cream containing the composition of palm oil ceramide, batyl alcohol, and ruscosaponin is mild and non-irritating.

[0073] Table 4 Irritation evaluation test results

[0074]

[0075]

[0076] Experimental Example 3: Barrier-related protein FLG test experiment

[0077] use Experiments were conducted on a 3D skin model. To prepare a 0.1% SLS working solution, pipette 1 mL of 0.2% SLS solution and add 1 mL of PBS to create a 0.1% SLS working solution. To prepare the positive control (WY14643) working solution, pipette 10 μL of 30 mM WY14643 stock solution into 6 mL of EpiGrowth culture medium to create a 50 μM WY14643 solution.

[0078] According to the test scheme in Table 5, the model was transferred to a 6-well plate (0.9 mL EpiGrowth culture medium was added in advance), and the test group number was marked on the 6-well plate. 25 μL of 0.1% SLS solution was added to the surface of the negative control group (NC), the positive control group (PC), Examples 1-4, and Comparative Examples 1-8, and incubated for 30 minutes. After the incubation, the working solution of the corresponding concentration was added to the positive control group, and the sample to be tested (the test sample of the sample of Example 1-4 and Comparative Example 1-8) was taken.

[0079] 12.5 μL was added to the model surface, evenly coated, and then placed in a CO2 incubator (37°C, 5% CO2) for 24 hours. After incubation, the residual test substance on the model surface was washed with sterile PBS solution, and the residual liquid inside and outside the model was wiped with a sterile cotton swab.

[0080] Immunofluorescence test: Take the model for testing, remove it by circumcision, and fix it with 4% paraformaldehyde. After fixation for 24 hours, perform immunofluorescence detection of FLG content, observe it under a microscope, collect pictures and analyze them.

[0081] Graphs were generated using GraphPad Prism, and the results are expressed as mean ± SD. Statistical analysis was performed using the t-test for comparisons between groups. All statistical analyses were two-tailed. P < 0.05 was considered significant, and P < 0.01 was considered extremely significant.

[0082] Table 5: Test plan

[0083]

[0084]

[0085] Table 6: Summary of FLG content test results

[0086]

[0087] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control; * indicates p<0.01 compared with negative control <p<0.05。)

[0088] As can be seen from Table 6, Examples 1 to 4, Comparative Examples 1, and 3-7 significantly promoted FLG protein expression, while Comparative Example 2, a cream containing only batyl alcohol, and Comparative Example 8, a cream containing no composition, did not significantly promote FLG gene expression. The promotion rates of Examples 1-4 were significantly higher than those of Comparative Examples 1 and 3-7, and the promotion rate of Example 3 was higher than the sum of the promotion rates of Comparative Examples 1-3, indicating that the composition of ceramides from tricholoma oil, batyl alcohol, and ruscosapogenin can synergistically promote FLG protein expression. The promotion rate of Example 3 was significantly higher than that of Comparative Example 7, indicating that the synergistic effect of the composition of ceramides from tricholoma oil, batyl alcohol, and ruscosapogenin is superior to that of the composition of ceramides from seabuckthorn oil, batyl alcohol, and ruscosapogenin.

[0089] Test Example 4: Laminin (LN) test experiment

[0090] Cells in the logarithmic growth phase were digested and centrifuged, and a cell suspension of appropriate density was prepared in complete medium. The suspension was seeded into 6-well cell culture plates according to the experimental design (Table 7) and incubated in a 37°C, 5% CO2 incubator for 24 ± 2 hours. Complete medium was added to the blank control group, complete medium containing 40 μM resveratrol was added to the positive control group, and complete medium containing the sample group containing the sample at a specific concentration was added to each well of the 6-well plate. The plates were then incubated in a CO2 incubator for 24 ± 2 hours. After incubation, the culture medium was discarded, the plates were washed three times with Dulbecco's phosphate-buffered saline (DPBS), and Western blot analysis was performed.

[0091] The measurement data were expressed as mean ± standard deviation (X ± s), and the data were subjected to one-way analysis of variance. The P value was used to judge the statistical difference, and P < 0.05 indicated statistical significance.

[0092] Laminin up-regulation rate (%) = (laminin expression level in sample group - laminin expression level in blank control group) / laminin expression level in blank control group * 100

[0093] Table 7 Test plan

[0094]

[0095] Table 8 Summary of laminin expression

[0096]

[0097] (Note: ** indicates p < 0.01 compared with blank control; * indicates p < 0.01 compared with blank control <p<0.05。)

[0098] As can be seen from Table 8, compared with the BC group, the laminin expression level in the PC group increased significantly, indicating that the positive control of this test is effective. Compared with the BC group, Examples 1 to 4, Comparative Examples 1, 2, and 4-7 have a significant effect on promoting laminin expression. Comparative Example 3 containing only ruscosapogenin and Comparative Example 8, a cream containing no ceramides, batyl alcohol, or ruscosapogenin, do not have a significant effect on promoting laminin expression. The promotion rates of Examples 1-4 are significantly higher than those of Comparative Examples 1, 2, and 4-7, and the promotion rate of Example 3 is higher than the sum of the promotion rates of Comparative Examples 1-3, indicating that the ceramides, batyl alcohol, and ruscosapogenin combination can synergistically promote laminin expression. The promotion rate of Example 3 is significantly higher than that of Comparative Example 7, indicating that the synergistic effect of the ceramides, batyl alcohol, and ruscosapogenin combination is superior to that of the seabuckthorn oil ceramides, batyl alcohol, and ruscosapogenin combination.

[0099] Test Example 5: Evaluation of the human body repair and anti-aging efficacy

[0100] Example 3 was tested for its efficacy in repairing and anti-aging human skin. A total of 30 subjects completed the test, including 8 males and 22 females, aged 30 to 45 years old, with an average age of 32.15±5.50, meeting the voluntary inclusion and exclusion criteria.

[0101] How to use: After using toner, take an appropriate amount of sample and apply it evenly on the face, pat until absorbed, and then carry out normal skin care steps. Use it once in the morning and evening every day. Other skin care habits remain unchanged and use it continuously for 28 days.

[0102] Detection time: before using the sample (D0), 7 days after using the sample (D7), and 28 days after using the sample (D28).

[0103] Testing instruments: Vapormeter SWL5001, skin water loss tester, and Cutometer MPA580, skin elasticity tester (CK, Germany).

[0104] Test requirements: Test 3 times and take the average value.

[0105] Parameter explanation: The lower the TEWL value, the less transepidermal water loss; the higher the R2 and R7 values, the firmer the skin and the better the anti-aging effect.

[0106] The results are shown in Tables 9-11.

[0107] Table 9 Descriptive statistics of TEWL values (n=30)

[0108]

[0109] Statistical analysis results: "**": The difference is statistically significant (0.001≤P<0.01); "***": The difference is statistically significant (P<0.001)

[0110] Table 10 Descriptive statistics of skin elasticity R2 value (n=30)

[0111]

[0112] Statistical analysis results: "**": The difference is statistically significant (0.001≤P<0.01); "***": The difference is statistically significant (P<0.001)

[0113] Table 11 Descriptive statistics of skin elasticity R7 value (n=30)

[0114]

[0115] Statistical analysis results: "***": The difference is statistically significant (P<0.001)

[0116] As shown in Tables 9-11, the skin TEWL value decreased significantly after 7 days and 28 days of use, the skin elasticity R2 increased significantly after 7 days and 28 days of use, and the skin elasticity R7 increased significantly after 7 days and 28 days of use. The results show that the cream containing the combination of ceramides from palm fruit oil, batyl alcohol, and ruscosaponin has significant effects on human body repair and anti-aging.

[0117] It should be noted that the above examples are only intended to illustrate the technical solutions of the present invention and are not intended to limit the same. Although the present invention has been described in detail with reference to the examples given, those skilled in the art may modify or replace the technical solutions of the present invention as needed without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A cream containing palm oil ceramide, characterized in that: The cream contains at least the following components in parts by mass: 0.1-5 parts of palm oil ceramide, 0.05-0.5 parts of batyl alcohol, and 0.01-0.1 parts of ruscosapogenin.

2. The cream according to claim 1, wherein The components include the following components in parts by mass: 1.5-3 parts of palm oil ceramide, 0.2-0.4 parts of batyl alcohol, and 0.05-0.08 parts of ruscosapogenin.

3. The cream according to claim 1 or 2, characterized in that The cream also contains cosmetic auxiliary ingredients commonly used in creams, including emulsifiers, oils, polyols, thickeners, preservatives, chelating agents and solvents.

4. The cream according to claim 1 or 2, characterized in that The ceramide of the tomentosa fruit oil is obtained by reacting fatty acids of the tomentosa fruit oil with sphingosine compounds.

5. The cream according to claim 4, characterized in that The sphingosine compound is selected from sphingosine, phytosphingosine or dihydrosphingosine.

6. The cream according to claim 4, characterized in that The palm oil fatty acid is obtained by saponification reaction using palm oil as a raw material.

7. The cream according to claim 1, wherein The cream contains at least the following components in parts by mass: 0.1-5 parts of palm oil ceramide, 0.05-0.5 parts of batyl alcohol, 0.01-0.1 parts of ruscosapogenin, 0.3-5.0 parts of emulsifier, 5.0-20.0 parts of oil, 2.0-10.0 parts of polyol, 0.1-1.0 parts of thickener, 0.3-1.5 parts of preservative, 0.05-0.20 parts of chelating agent, 90-100 parts of water; Preferably, the cream contains at least the following components in parts by mass: 1.5-3 parts of palm oil ceramide, 0.2-0.4 parts of batyl alcohol, 0.05-0.08 parts of ruscosapogenin, 2.0-5.0 parts of emulsifier, 6.0-12.0 parts of oil, 3.0-8.0 parts of polyol, 0.5-1.0 parts of thickener, 0.3-0.8 parts of preservative, 0.05-0.15 parts of chelating agent, and 93-98 parts of water.

8. The method for preparing the cream containing palm oil ceramide according to any one of claims 1 to 7, characterized in that: The preparation method comprises: S1. Preparation of aqueous phase: Mix polyol, thickener, chelating agent, aqueous emulsifier and water, heat to 75-80°C and stir to dissolve; S2. Preparation of oil phase: Heat the oil phase emulsifier and oil to 70-75°C to melt. After complete melting, add palm oil ceramide and batyl alcohol before mixing, and stir until uniformly melted. S3, homogenizing the aqueous phase and the oil phase; adding ruscosapogenin after cooling; further, the ruscosapogenin is pre-heated with butanediol to 85° C. and stirred to dissolve before adding; S4: Cool down to 65-70℃, add preservative, stir to dissolve, then stir and cool down to about 38℃.

9. Use of a cream containing the palm oil ceramide according to any one of claims 1 to 7 in the preparation of cosmetics.

10. A cosmetic, characterized in that: The cosmetic comprises at least the cream containing palmetto fruit oil ceramide according to any one of claims 1 to 7; further, the cosmetic is a skin care product.

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