Time-sharing repair formula based on circadian rhythm and application thereof

Abstract

The invention discloses a time-sharing repair formula based on circadian rhythm and application, a daytime composition takes oxidation resistance, compression resistance and energy metabolism as cores, resists oxidative damage, improves daytime vitality and provides long-acting anti-oxidation protection, and a night composition takes DNA repair, anti-inflammation and nerve soothing as cores, promotes cell regeneration and repair, continuously supports night repair, and has the functions of preventing and treating circadian rhythm. The composition of the daytime composition and the nighttime composition is designed according to the circadian rhythm of human metabolism, so that the corresponding activity plays a role in the optimal period of time, and the components are designed according to different physiological needs of daytime / nighttime to cooperate with each other, so that the anti-aging effect is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of human health care, and in particular to a time-sharing repair formula based on circadian rhythm and application thereof. Background Art

[0002] Anti-aging products, such as chemically synthesized preparations, often suffer from significant side effects. Plant-derived anti-aging ingredients offer significant safety advantages and are more readily accepted by consumers. There are a wide variety of anti-aging products on the market, but most are formulated with a single formula for all-day use, such as vitamin complexes and antioxidants. These ingredients fail to fully match the diurnal fluctuations in human metabolism, such as during the daytime, when oxidative stress peaks. Common anti-aging products focus on antioxidant design during this time, but at night, human cells enter a window for DNA repair and regeneration. Conventional anti-aging formulas lack repair-supporting ingredients, resulting in low repair efficiency and a need for improvement. Summary of the Invention

[0003] To solve at least one of the above technical deficiencies, the present invention provides the following technical solutions:

[0004] The first aspect of this application document discloses a time-sharing repair formula based on circadian rhythm, comprising:

[0005] The daytime composition for daytime use is composed, by mass, of 15-20 parts of pyrroloquinoline quinone (PQQ), 15-20 parts of ergothioneine, 5-9 parts of wheat germ extract, 15-20 parts of B vitamins, 5-10 parts of Rhodiola rosea extract, 3-6 parts of yeast powder containing superoxide dismutase (SOD), 35-55 parts of ginseng extract, 120-160 parts of taurine, and 100-160 parts of the first auxiliary material.

[0006] The nighttime composition for nighttime use is composed, by mass, of 120-160 parts of gamma-aminobutyric acid, 2-6 parts of ashwagandha extract, 100-150 parts of casein hydrolyzed peptide, 5-9 parts of jujube seed extract, 5-9 parts of black pepper extract, 12-20 parts of curcumin, and 100-160 parts of the second auxiliary material.

[0007] Furthermore, the Rhodiola rosea extract is a substance extracted from the root of Rhodiola rosea by an alcohol precipitation method; the wheat germ extract is a substance obtained by treating wheat germ by a dry method; the Ashwagandha extract is a substance obtained by solvent extraction, concentration, and drying of Ashwagandha; the black pepper extract is obtained by solvent extraction of black pepper powder; and the Ziziphus jujuba seed extract is obtained by extraction of Ziziphus jujuba seed.

[0008] Furthermore, the B vitamin is vitamin B2, and the SOD content in the SOD-containing yeast powder is 50,000-500,000 IU / g.

[0009] Furthermore, the first auxiliary material includes tricalcium phosphate and microcrystalline cellulose, and the mass ratio of tricalcium phosphate to microcrystalline cellulose is 1-3:6-8; the second auxiliary material includes silicon dioxide and tricalcium phosphate, and the mass ratio of silicon dioxide to tricalcium phosphate is 2-6:1.

[0010] Furthermore, the daytime composition and the nighttime composition are each granulated.

[0011] The second aspect of this application document discloses the application of the above-mentioned time-sharing repair formula in anti-aging preparations.

[0012] Here’s how it works:

[0013] Daytime combination: Focusing on anti-oxidation, anti-stress and energy metabolism, it resists oxidative damage and enhances daytime vitality, providing long-lasting antioxidant protection.

[0014] Nighttime composition: Focusing on DNA repair, anti-inflammation and nerve relief, it promotes cell regeneration and repair, and continuously supports nighttime repair.

[0015] Among them, the function of PQQ is: mitochondrial biosynthesis activator, which increases the energy metabolism rate.

[0016] Function of ergothioneine: strong antioxidant, inhibits the generation of reactive oxygen species (ROS).

[0017] Wheat germ extract: natural source of vitamin E, synergistic antioxidant.

[0018] B vitamins: coenzymes for energy metabolism, relieve fatigue.

[0019] Rhodiola rosea extract: an anti-stress adaptogen that improves daytime stress resistance.

[0020] Contains SOD yeast powder: superoxide dismutase, which removes free radicals.

[0021] GABA: neurotransmitter regulation, promotes deep sleep.

[0022] Ashwagandha Extract: Cortisol regulation, reducing nighttime inflammatory response.

[0023] Casein hydrolyzed peptides: Promote the expression of DNA repair proteins, such as DNA repair enzymes (PARP).

[0024] Ziziphus jujuba seed extract: a precursor of melatonin, regulates sleep rhythm.

[0025] Curcumin: Anti-inflammatory and autophagy activation, clearing damaged cells.

[0026] The synergistic antioxidant effect of ergothioneine and yeast powder containing SOD increases the efficiency of scavenging free radicals by 40% compared with a single ingredient. The combined use of casein hydrolyzed peptides and curcumin increases the DNA repair rate by 30%.

[0027] The combination of Rhodiola rosea extract + taurine significantly enhances daytime energy, while GABA + Ziziphus jujuba seed extract shortens the time to fall asleep to within 15 minutes.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention designs the composition of the daytime and nighttime compositions based on the circadian rhythm of human metabolism, allowing the corresponding activities to take effect at the optimal time. The ingredients are designed to work synergistically based on the different physiological needs of the day / night, which helps to significantly enhance the anti-aging effect. DETAILED DESCRIPTION

[0030] The present invention will be further described below with reference to specific embodiments.

[0031] Casein hydrolyzed peptides (protein ≥75%, fat ≤1%, moisture ≤7.0, ash ≤8.0%) were provided by Gansu Hua'an Biotechnology Group, China; SOD-containing yeast powder (SOD-100,000 IU / g) was provided by Shaanxi Senyuan Biotechnology Co., Ltd., China; ginseng extract was provided by Jilin Hongjiu Biotechnology Co., Ltd., China, with oil solubility 40 mg / 100 ml, water solubility 0.8 g / 100 ml, and density 50 mg / L.

[0032] Rhodiola rosea extract, salidroside ≥3%, provided by Xi'an Tianyi Biotechnology Co., Ltd., China, extracted from the roots of Rhodiola rosea by alcohol precipitation; wheat germ extract (spermidine 0.8%), provided by Xi'an Binghe Biotechnology Co., Ltd., China, obtained by dry processing wheat germ; Withania somnifera extract, withanolide 6%, provided by Shaanxi Fuheng Biotechnology Co., Ltd., China, obtained by solvent extraction, concentration, and drying of Withania somnifera; Ziziphus jujuba seed extract, 2% total saponins from Ziziphus jujuba seed, provided by Xi'an Tianyi Biotechnology Co., Ltd., China, obtained by extraction of Ziziphus jujuba seed; black pepper extract, piperine 95%, provided by Hanzhong Natural Valley Biotechnology Co., Ltd., China, obtained black pepper powder by solvent extraction.

[0033] Example 1

[0034] A circadian rhythm-based time-sharing repair formula, including:

[0035] The daytime composition is composed, by mass, of 16 parts of PQQ, 17 parts of ergothioneine, 6 parts of wheat germ extract, 16 parts of vitamin B2, 7 parts of Rhodiola rosea extract, 5 parts of SOD-containing yeast powder, 40 parts of ginseng extract, 130 parts of taurine, and 120 parts of the first auxiliary material, wherein the first auxiliary material is tricalcium phosphate and microcrystalline cellulose, and the mass ratio of tricalcium phosphate to microcrystalline cellulose is 1:4. The components are mixed and granulated.

[0036] Nighttime composition: composed of 140 parts of γ-aminobutyric acid, 4 parts of ashwagandha extract, 120 parts of casein hydrolyzed peptide, 5 parts of jujube seed extract, 7 parts of black pepper extract, 15 parts of curcumin, and 120 parts of the second auxiliary material, and each component is measured by mass, wherein the second auxiliary material is silicon dioxide and tricalcium phosphate, and the mass ratio of silicon dioxide to tricalcium phosphate is 3:1. The components are mixed and granulated.

[0037] Example 2

[0038] A circadian rhythm-based time-sharing repair formula, including:

[0039] The daytime composition is composed, by mass, of 18 parts of PQQ, 16 parts of ergothioneine, 7 parts of wheat germ extract, 18 parts of vitamin B2, 6 parts of Rhodiola rosea extract, 6 parts of SOD-containing yeast powder, 45 parts of ginseng extract, 140 parts of taurine, and 130 parts of the first auxiliary material, wherein the first auxiliary material is tricalcium phosphate and microcrystalline cellulose, and the mass ratio of tricalcium phosphate to microcrystalline cellulose is 1:3. The components are mixed and granulated.

[0040] Nighttime composition: calculated by mass, consisting of 150 parts of γ-aminobutyric acid, 5 parts of ashwagandha extract, 130 parts of casein hydrolyzed peptide, 6 parts of jujube seed extract, 8 parts of black pepper extract, 18 parts of curcumin, and 130 parts of a second auxiliary material, wherein the second auxiliary material is silicon dioxide and tricalcium phosphate, and the mass ratio of silicon dioxide to tricalcium phosphate is 4:1. The components are mixed and granulated.

[0041] Example 3

[0042] A circadian rhythm-based time-sharing repair formula, including:

[0043] The daytime composition is composed, by mass, of 20 parts of PQQ, 20 parts of ergothioneine, 7 parts of wheat germ extract, 18 parts of vitamin B2, 8 parts of Rhodiola rosea extract, 6 parts of SOD-containing yeast powder, 55 parts of ginseng extract, 160 parts of taurine, and 140 parts of the first auxiliary material, wherein the first auxiliary material is tricalcium phosphate and microcrystalline cellulose, and the mass ratio of tricalcium phosphate to microcrystalline cellulose is 1:5. The components are mixed and granulated.

[0044] Nighttime composition: calculated by mass, consisting of 160 parts of γ-aminobutyric acid, 6 parts of ashwagandha extract, 150 parts of casein hydrolyzed peptide, 8 parts of jujube seed extract, 8 parts of black pepper extract, 19 parts of curcumin, and 150 parts of a second auxiliary material, wherein the second auxiliary material is silicon dioxide and tricalcium phosphate, and the mass ratio of silicon dioxide to tricalcium phosphate is 2:1. The components are mixed and granulated.

[0045] Example 4

[0046] A circadian rhythm-based time-sharing repair formula, including:

[0047] The daytime composition is composed, by mass, of 16 parts of PQQ, 19 parts of ergothioneine, 8 parts of wheat germ extract, 15 parts of vitamin B2, 9 parts of Rhodiola rosea extract, 4 parts of SOD-containing yeast powder, 38 parts of ginseng extract, 125 parts of taurine, and 110 parts of the first auxiliary material, wherein the first auxiliary material is tricalcium phosphate and microcrystalline cellulose, and the mass ratio of tricalcium phosphate to microcrystalline cellulose is 1:2. The components are mixed and granulated.

[0048] Nighttime composition: calculated by mass, consisting of 147 parts of γ-aminobutyric acid, 4 parts of ashwagandha extract, 144 parts of casein hydrolyzed peptide, 7.5 parts of jujube seed extract, 8 parts of black pepper extract, 12 parts of curcumin, and 135 parts of a second auxiliary material, wherein the second auxiliary material is silicon dioxide and tricalcium phosphate, and the mass ratio of silicon dioxide to tricalcium phosphate is 3:1. The components are mixed and granulated.

[0049] Comparative Example 1

[0050] Compared with Example 1, the difference is that the daytime composition does not contain SOD-containing yeast powder, and the nighttime composition does not contain curcumin.

[0051] Comparative Example 2

[0052] Compared with Example 1, the difference is that the daytime composition does not contain Rhodiola rosea extract, and the nighttime composition does not contain Ziziphus jujuba extract.

[0053] Human testing was conducted using the daytime and nighttime compositions prepared in Examples 1-4 and Comparative Examples 1-2 as anti-aging preparations. Sixty healthy volunteers were divided into two groups. Inclusion criteria included: First, both men and women, aged 30-65 years; Second, good health, a serious attitude, and the ability to express themselves authentically, able to truly reflect their feelings after use; Third, voluntary participation, signing an informed consent form, and strict adherence to the study protocol, using the product as required, and completing follow-up visits. Exclusion criteria included: First, pregnant or breastfeeding women; Second, patients with facial injuries or skin diseases; Third, patients undergoing facial skin treatments, cosmetic procedures, or other testing; Fourth, patients with sunburn or hormonal medication use within the past month; Fifth, patients undergoing other facial clinical studies within the past three months. Termination and exclusion criteria included: First, the subject requested to discontinue the study; Second, adverse reactions or special physiological and pathological changes; Third, the use of other skin care products that could affect the study.

[0054] Long-term test: Volunteers took one granule prepared from the daytime composition with breakfast during the day, each granule was 260 mg, and took one granule prepared from the nighttime composition 30 minutes before bedtime, each granule was 260 mg. One side was followed up at 0 week before use, 2 weeks after use, 4 weeks after use, 6 weeks after use, and 8 weeks after use, and each follow-up was done at the same time during the day.

[0055] The facial elasticity test was performed using a skin elasticity tester, as shown in the following table, wherein Table 1 shows the results of group 1 volunteers taking the granules prepared in Example 1, and Table 2 shows the results of group 2 volunteers taking the granules prepared in Comparative Example 1.

[0056] Table 1

[0057]

[0058] Table 2

[0059]

[0060] It can be seen that after the 4th week, the volunteers taking Example 1 have significantly improved skin elasticity, while the skin elasticity growth is relatively low in Comparative Example 1. It has been found that: in the daytime composition, ergothioneine and SOD yeast powder have antioxidant synergistic effects, and compared with a single component, the two cooperate to remove free radicals. Efficiency is improved by 40%. In the nighttime composition, casein hydrolyzed peptides and curcumin have a synergistic effect, and compared with a single component, the two cooperate to improve DNA repair rate by 30%.

[0061] In addition, according to the feedback from the volunteers, compared with the comparative example 2, taking the granules prepared by the daytime composition of Example 1 can significantly enhance daytime energy, indicating that the combination of Rhodiola rosea extract and taurine has an excellent effect.

[0062] In addition, according to feedback from volunteers, compared with Comparative Example 2, taking the granules prepared from the daytime composition of Example 1 can significantly shorten the time to fall asleep to within 15 minutes. The average time to fall asleep for the volunteers taking Comparative Example 2 is 25 minutes.

[0063] The above are merely preferred embodiments of the present invention. The scope of protection of the present invention is not limited to the above embodiments. All technical solutions based on the principles of the present invention are within the scope of protection of the present invention. It should be noted that improvements and modifications that do not depart from the principles of the present invention are within the scope of protection of the present invention.

Claims

1. A time-sharing repair formula based on circadian rhythm, including: A daytime composition for daytime use: composed of 15-20 parts of PQQ, 15-20 parts of ergothioneine, 5-9 parts of wheat germ extract, 15-20 parts of B vitamins, 5-10 parts of Rhodiola rosea extract, 3-6 parts of SOD-containing yeast powder, 35-55 parts of ginseng extract, 120-160 parts of taurine, and 100-160 parts of the first auxiliary material, calculated by mass; The nighttime composition for nighttime use is composed of 120-160 parts of gamma-aminobutyric acid, 2-6 parts of ashwagandha extract, 100-150 parts of casein hydrolyzed peptide, 5-9 parts of jujube seed extract, 5-9 parts of black pepper extract, 12-20 parts of curcumin, and 100-160 parts of the second auxiliary material, calculated by mass.

2. The circadian rhythm-based time-sharing repair formula according to claim 1, wherein: The Rhodiola rosea extract is a substance extracted from the roots of Rhodiola rosea by an alcohol precipitation method; the wheat germ extract is a substance obtained by treating wheat germ by a dry method; the Ashwagandha extract is a substance obtained by solvent extraction, concentration, and drying of Ashwagandha; the black pepper extract is obtained by solvent extraction of black pepper powder; and the Ziziphus jujuba seed extract is obtained by extraction of Ziziphus jujuba seeds.

3. The circadian rhythm-based time-sharing repair formula according to claim 1, wherein: The B vitamin is vitamin B2, and the SOD content in the SOD-containing yeast powder is 50,000-500,000 IU / g.

4. The time-sharing repair formula based on circadian rhythm as claimed in claim 1, wherein: The first auxiliary material includes tricalcium phosphate and microcrystalline cellulose, and the mass ratio of tricalcium phosphate to microcrystalline cellulose is 1-3:6-8; the second auxiliary material includes silicon dioxide and tricalcium phosphate, and the mass ratio of silicon dioxide to tricalcium phosphate is 2-6:

1.

5. The circadian rhythm-based time-sharing repair formula according to claim 1, wherein: The daytime composition and the nighttime composition are granulated separately.

6. Use of the time-sharing repair formula according to any one of claims 1 to 5 in an anti-aging preparation.

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