Carboxylic acid reductase DeCAR recombinant plasmid and application thereof in preparation of aldehyde spices

By constructing the DeCAR recombinant plasmid to catalyze the acid production of aldehydes in E. coli, Aspergillus oryzae and Saccharomyces cerevisiae, the problem of synthesis of aldehyde fragrances in the prior art is solved, and efficient and environmentally friendly preparation of aldehyde fragrances is achieved.

CN120555474APending Publication Date: 2025-08-29NANJING UNIV OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Application Number
CN202410223410.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-02-28
Publication Date
2025-08-29

AI Technical Summary

Technical Problem

The existing chemical method of catalyzing the preparation of aldehydes from acid reduction requires noble metal catalysts, multiple steps, extreme reaction conditions, poor tolerance to adjacent functional groups, and limited research on the CAR gene of carboxylic acid reductase, making it difficult to efficiently synthesize aldehyde fragrances.

Method used

The DeCAR recombinant plasmid from C. pyroxyl Carbohydrate was used to catalyze the acid production of aldehydes in E. coli, Aspergillus oryzae and Saccharomyces cerevisiae. The catalytic activity was enhanced by using the phosphopanomide transferase PPTase to construct the recombinant plasmids on pGS21α, pETDuet-1, pTAex3 and pYET vectors.

Benefits of technology

It has achieved efficient biological preparation of aldehyde fragrances, with a wide substrate spectrum and high catalytic activity. It is suitable for the efficient synthesis of aromatic acids and fatty acids, and is economical, green and environmentally friendly.

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Abstract

The invention discloses a carboxylic acid reductase DeCAR recombinant plasmid and application of the carboxylic acid reductase DeCAR recombinant plasmid in preparation of aldehyde spices, belongs to the technical field of bioengineering, and further provides a recombinant bacterium containing the recombinant plasmid, and the carboxylic acid reductase DeCAR is further produced by using the recombinant bacterium, or the recombinant bacterium is directly applied to production of the aldehyde spices. Compared with other biocatalysts for preparing aldehydes, the carboxylic acid reductase and the recombinant bacteria thereof provided by the invention have the advantages of wide substrate spectrum, high catalytic activity, strong substrate specificity and the like, and have wide application prospects in industrial application.
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Citation Information

Cited By

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