Pharmaceutical application of artemisia plant essential oil

Abstract

The invention belongs to the field of plant essential oil, and particularly relates to pharmaceutical application of artemisia plant essential oil. The specific technical scheme is as follows: according to the pharmaceutical application of the artemisia plant essential oil, the artemisia plant essential oil is extracted from wormwood growing above 2000 m, and can inhibit or lower TNF signal channels, interaction between cell factors and cell factor receptors, NOD-like receptor signal channels, Toll-like receptor signal channels, cell adhesion molecule signal channels and other channels, so that the artemisia plant essential oil can be used for inhibiting or lowering the TNF signal channels, the interaction between the cell factors and the cell factor receptors, the NOD-like receptor signal channels, the Toll-like receptor signal channels, the cell adhesion molecule signal channels and the like. Therefore, wound healing is promoted, inflammation is resisted, and scar hyperplasia is reduced or avoided.

Description

Technical Field

[0001] The invention belongs to the field of plant essential oils, and particularly relates to pharmaceutical use of Artemisia plant essential oil. Background Art

[0002] Wound healing generally occurs in three stages: inflammation, proliferation, and tissue remodeling. During the wound healing process, pathological scars may form, causing changes in the skin's appearance. This can not only lead to psychological problems such as low self-esteem, anxiety, and depression, but can also cause dysfunction in vital areas like the joints and rib cage, further impacting daily activities and respiratory function. These wounds are also characterized by high repair costs, prolonged recovery times, and a high recurrence rate. Currently, there is a lack of effective products on the market that can both promote wound healing and inhibit scar proliferation.

[0003] Mugwort, a plant of the genus Artemisia in the Asteraceae family, is used as a medicinal herb. Its medicinal properties include warming the meridians, detoxifying and removing dampness, dispelling cold and relieving pain, stopping bleeding and stabilizing pregnancy, repelling mosquitoes and insects, and inhibiting bacteria. Compared to mugwort from other origins, mugwort from the Daliangshan Plateau boasts a unique growing environment and exhibits distinct appearance differences, including thicker, larger, and more lush leaves and a purplish-red stalk. However, its current utilization remains limited, and its potential for medical and health applications remains to be explored. Summary of the Invention

[0004] The present invention aims to provide a pharmaceutical use of Artemisia plant essential oil.

[0005] To achieve the above-mentioned purpose of the invention, the technical solution adopted by the present invention is: an application of an Artemisia essential oil in the preparation of a drug that promotes wound healing and / or anti-inflammatory, wherein the components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, borneol, bicyclogerene and epoxidized humulene II, and the application of the Artemisia essential oil in the preparation of a drug that downregulates or inhibits the TNF signaling pathway, downregulates or inhibits the interaction between cytokines and cytokine receptors, downregulates or inhibits the NOD-like receptor signaling pathway, downregulates or inhibits the Toll-like receptor signaling pathway, and downregulates or inhibits the cell adhesion molecule signaling pathway.

[0006] Correspondingly, an Artemisia essential oil is used in the preparation of a drug for reducing or preventing the formation of hypertrophic scars. The components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, borneol, bicyclogerene and epoxidized humulene II. The Artemisia essential oil is used in the preparation of a drug for downregulating or inhibiting the TGF-β signaling pathway and downregulating or inhibiting the cell cycle signaling pathway.

[0007] Accordingly, a drug capable of promoting wound healing, anti-inflammation, and reducing or preventing the formation of hypertrophic scars comprises an Artemisia essential oil, wherein the components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogerene, and epoxidized humulene II.

[0008] The present invention has the following beneficial effects: It provides pharmaceutical uses of Daliangshan Plateau Artemisia annua essential oil, which can both promote wound healing and inhibit scar hyperplasia. The essential oil not only regulates multiple signaling pathways associated with inflammation during the wound healing phase, but also regulates multiple signaling pathways associated with scar formation during the scar hyperplasia phase. Therefore, the essential oil provided by the present invention can be used not only to prepare drugs that regulate multiple signaling pathways, but also has the potential to be used to prepare drugs that can simultaneously promote wound healing and prevent scar hyperplasia. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 This is the total ion map of the blank control group;

[0010] Figure 2 This is the total ion map of Liangshan Artemisia argyi essential oil from Zire Village, Puge County;

[0011] Figure 3 This is the total ion map of Liangshan Artemisia argyi essential oil from Zire Village, Puge County;

[0012] Figure 4 This is the total ion map of Liangshan Artemisia annua essential oil from Boluoping Village, Puge County. DETAILED DESCRIPTION

[0013] The present invention provides an Artemisia essential oil extracted from plateau mugwort (mugwort grown at altitudes above 2,000 meters, preferably in the Daliang Mountains). The essential oil is prepared by washing the surface portion of freshly picked mugwort, placing it in pure water, and distilling it at 85°C to 100°C for 1 to 5 hours. The volume ratio of mugwort to pure water is 1:10 to 100.

[0014] The active ingredients in the essential oil include eucalyptol, α-thujone, β-caryophyllene, chamazulene, L-borneol, bicyclogeran and epoxidized humulene II; in the essential oil, the eucalyptol content is greater than 18% (v / v), the α-thujone content is greater than 1.0% (v / v), the β-caryophyllene content is greater than 5% (v / v), the chamazulene content is greater than 0.03% (v / v), and the L-borneol content is greater than 3.5% (v / v).

[0015] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, rather than all embodiments. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art. The data obtained are all average values ​​obtained after at least 3 repetitions, and all data obtained in each repetition are valid data.

[0016] Example 1: Extraction of Artemisia essential oil

[0017] Essential oil was extracted from mugwort grown at altitudes above 2,000 meters, specifically from Luojishanzire Village in Puge County, Qingshuihe Village in Mianya Town, Yanyuan County, and Boluoping Village in Puge County. The extraction method involves washing the above-ground portion of freshly picked mugwort, placing it in pure water, and distilling it at 100°C for 5 hours. The volume ratio of mugwort to pure water is 1:50. The resulting mugwort essential oil is blue in color.

[0018] Example 2: Analysis of Artemisia essential oil components

[0019] 1. The main chemical components of the essential oils of Artemisia argyi obtained from the three regions in Example 1 were qualitatively analyzed by GC-MS to determine their relative percentages, and a blank control group was set up (1 μL of n-hexane was used instead of the sample to be tested, with the other conditions being the same). The specific method includes:

[0020] Dissolve 20 μL of each essential oil sample in n-hexane, dilute to 1000 μL, and shake well. Accurately measure 100 μL of this solution and dilute again to 1000 μL with n-hexane. Shake well to obtain the test sample. Accurately pipette 1 μL of the test sample and inject it into the gas chromatography-mass spectrometer (GC-MS). Scan and obtain a mass spectrum.

[0021] The gas chromatography conditions were as follows: chromatographic column: Agilent DB-5MS (30m×0.25mm×0.25μm); carrier gas: high-purity helium (1.0mL / min); programmed temperature (60°C for 3 min; 100°C at a rate of 10°C / min, for 2 min; 125°C at a rate of 5°C / min, then 180°C at a rate of 7°C / min, then 280°C at a rate of 10°C / min, for 5 min); injection port temperature: 250°C; detector temperature: 280°C; split ratio: 30:1. Mass spectrometry conditions: electron bombardment ion source; electron energy: 70eV; ion source temperature: 200°C; interface temperature: 280°C; scan range: m / z 40-600; full scan mode. The fingerprint spectrum obtained is as follows: Figures 1 to 4 shown.

[0022] Figure 1This is the blank control group. It can be seen that the blank reagent (n-hexane) has basically no interference with the detection. Figures 2-4 These are the fingerprint maps of three regions. It can be seen that the main components of the essential oils extracted from mugwort from different regions are basically the same.

[0023] 2. Yes Figures 2-4 The top 100 substances by peak area were integrated, and their relative percentages were calculated using peak area normalization. These were then searched and matched against the NIST spectral library. The main active ingredients in essential oils from various regions are shown in Table 1.

[0024] Table 1 Comparison of main components in essential oils

[0025]

[0026]

[0027] The active ingredients in the essential oil are mainly terpenes, with high contents of eucalyptol, β-caryophyllene, (-)-terpinen-4-ol, D(+)-camphor and α-terpineol; the matricaria (blue oily azulene-like substance) in the essential oil may be the main chemical component that makes it blue; two components, (+)-bicyclogermelene and epoxidized humulene II, were identified in mugwort for the first time, which may give the essential oil anti-cancer, antibacterial and anti-inflammatory effects, or enhance these effects of the essential oil.

[0028] Example 3: Demonstration of the effects of Artemisia essential oil

[0029] The pathways measured in this example are all standard KEGG pathway names.

[0030] 1. Regulatory effects on key genes and signaling pathways for wound healing and anti-inflammatory

[0031] HUVEC1 cells (transformed human umbilical vein endothelial cells) were stimulated with 5 ng / mL of IL-1β for 24 h to simulate the vascular inflammation state under pathological conditions and construct a vascular endothelial cell inflammation model.

[0032] HUVEC1 cells in the logarithmic growth phase were seeded at a density of 3,000 cells / well in a cell culture plate in DMEM (containing 1% NEAA, a non-essential amino acid mixture) supplemented with 10% fetal bovine serum. The cells were cultured in a 37°C incubator for 24 hours. The cells were then removed and photographed using a microscope to record their status before drug addition.

[0033] After observing good cell adhesion under a microscope, aspirate the culture medium from each well. Add 5 ng / mL of IL-1β to induce a vascular endothelial cell inflammation model. Simultaneously with modeling, add DMEM culture medium containing varying concentrations of drugs and return to a 37°C incubator for 24 hours. Simultaneously, establish a model group, in which only the modeling agent is added without any drugs, and a blank group, in which normal culture is performed without the addition of IL-1β or any drugs. Set up three replicates for each group.

[0034] The drugs added to each group were as follows: (1) essential oil: the essential oil extracted from Zire Village, Luojishan Town, Puge County in Example 1 was diluted with dimethyl sulfoxide to a final concentration of 800 μg / mL; (2) thujone: purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a purity of 99.9%, diluted with dimethyl sulfoxide to a final concentration of 100 μM; (3) matricaria: purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a purity of 98.2%, diluted with dimethyl sulfoxide to a final concentration of 10 μM; (4) eucalyptol: purchased from China Food and Drug Administration with a purity of 99.7%, diluted with dimethyl sulfoxide to a final concentration of 200 μg / mL.

[0035] After the culture was completed, the culture medium in the cell culture plate was discarded, the cells were lysed and reverse transcribed into cDNA, and then amplified and purified by PCR. The library was then constructed using a double-end index. After the library samples passed the quality inspection, they were pooled and high-throughput sequencing was performed using the Illumina sequencing platform. Illumina HiSeq X10 was used to measure 2M reads for each sample, and the data was analyzed. Gene set enrichment analysis (GSEA) was used to perform enrichment analysis of the signal pathway. If the FDR (False Discovery Rate) was <0.25, the pathway was considered to be significantly regulated (compared with the blank group, the FDR was <0.25, and the pathway was considered to have changed significantly; compared with the model group, the FDR of each drug group was <0.25, and the pathway was considered to have changed significantly). The results are shown in Table 2. In Table 2, “ / ” indicates that there is no significant effect on the pathway.

[0036] Table 2 Comparison of the effects of different drugs on pathway regulation

[0037]

[0038] The results showed that although thujone and other substances can inhibit excessive inflammatory responses to a certain extent in terms of external manifestations, thereby avoiding pathological hyperplasia, their performance in pathway regulation is completely different from that of essential oils. The specific analysis is as follows:

[0039] (1) TNF signaling pathway. Compared with the blank group without vascular inflammation modeling, the TNF signaling pathway was activated in the model group. TNF (tumor necrosis factor) is a key proinflammatory cytokine. When blood vessels are inflamed, TNF levels rise. After binding to its receptor, it transmits signals and interacts with other molecules or signaling pathways to play a variety of biological roles, promoting the occurrence and development of inflammatory reactions. After TNF binds to TNFR1 (tumor necrosis factor receptor 1), it mediates proinflammatory effects, activates the NF-κB signaling pathway, promotes the expression of inflammatory factors, and participates in cell apoptosis and necrosis pathways. The release of these inflammatory factors will further aggravate vascular inflammation, leading to pathological changes such as vascular endothelial cell damage and increased vascular permeability. Therefore, in the model group, the TNF signaling pathway was activated, indicating that the vascular inflammation modeling was successful. By inhibiting the TNF signaling pathway, the transmission of inflammatory signals can be blocked, the activation and damage of inflammatory cells to vascular endothelial cells can be reduced, thereby helping to restore the normal function of vascular endothelial cells, reduce vascular permeability, and alleviate inflammatory reactions. After adding essential oils, the TNF signaling pathway is inhibited, which is expected to relieve inflammation. Thujone and chamoxadulone had no significant effect on the TNF signaling pathway, indicating that they could not relieve inflammation by inhibiting the TNF signaling pathway; after adding eucalyptol, the TNF signaling pathway was further promoted and activated, which may lead to further development of inflammation.

[0040] (2) Interaction between cytokines and cytokine receptors. Cytokines are a class of polypeptides or proteins secreted by cells that play a key role in intercellular communication and the regulation of immunity, inflammation, and tissue repair. In vascular inflammation, cytokines play an amplifying and regulating role, helping to eliminate pathogens and promote tissue repair, and promoting damage and inflammatory responses of vascular endothelial cells. Cytokines exert their effects by binding to specific receptors on the surface of target cells. When cytokines bind to receptors, they activate intracellular signal transduction pathways, regulate cell growth, differentiation, and function, and thus affect the occurrence and development of inflammatory responses. If the interaction between cytokines and receptors is inhibited, the cytokines cannot effectively transmit signals and thus cannot exert their pro-inflammatory effects. Compared with the blank group, the interaction between cytokines and cytokine receptors in the model group was enhanced, promoting the occurrence of inflammation. After adding essential oils, this interaction was inhibited and the inflammatory effect was reduced. Thujone and chamomile had no significant effect on the interaction between cytokines and cytokine receptors; after adding eucalyptol, the interaction between cytokines and cytokine receptors was further promoted, which may lead to further development of inflammation.

[0041] (3) NOD-like receptor signaling pathway. Inflammasome is a multiprotein complex that can activate caspase enzymes and promote the maturation and secretion of inflammatory factors. The NOD-like receptor signaling pathway involves a variety of signaling molecules and regulatory proteins, which can activate inflammatory responses. When the NOD-like receptor signaling pathway is inhibited, the activation of inflammasomes is blocked, the activation of caspase enzymes is reduced, and the maturation and secretion of inflammatory factors such as IL-1β and IL-18 are reduced, which in turn leads to a decrease in the maturation and secretion of inflammatory factors such as IL-1β and IL-18, and a weakening of the inflammatory response. The inhibition of the signaling pathway will also affect the activation and cell death process of immune cells, reduce the attack and damage of immune cells on vascular endothelial cells, inhibit cell necrosis and the aggravation of inflammatory response, thereby alleviating vascular inflammation. Compared with the blank group, the NOD-like receptor signaling pathway was activated in the model group, promoting the occurrence of inflammation. After adding essential oils or eucalyptol, this pathway was inhibited, which may slow down the inflammatory effect. Thujone had no significant effect on this pathway; after adding matricaria, it promoted this pathway, which may lead to further development of inflammation. It's important to note that while eucalyptol has no significant effect or even promotes inflammation in other tested inflammation-related pathways, it actually inhibits the NOD-like receptor signaling pathway, potentially suppressing inflammation. This is related to the complexity of signaling pathway regulation and the specificity of the substance. Therefore, just because a substance appears to promote wound healing or have anti-inflammatory effects doesn't necessarily mean it can inhibit a specific inflammation-promoting signaling pathway. Conversely, being able to inhibit or promote an inflammation-related signaling pathway doesn't necessarily mean it promotes wound healing or has anti-inflammatory effects.

[0042] (4) Toll-like receptor signaling pathway. Toll-like receptors (TLRs) are a type of pattern recognition receptors that are associated with inflammation and innate immune responses. They trigger innate immune responses by recognizing pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs), and regulate a variety of acute and chronic inflammatory diseases. Inhibiting the Toll-like receptor signaling pathway can block the intracellular immune response mediated by it, thereby reducing the transcription of pro-inflammatory cytokine-related genes and alleviating the inflammatory response. Compared with the blank group, the Toll-like receptor signaling pathway was activated in the model group, promoting the occurrence of inflammation. After adding essential oils, this pathway was inhibited, which may slow down the inflammatory effect. Thujone and chamomile had no significant effect on this pathway; after adding eucalyptol, it promoted this pathway, which may lead to further development of inflammation.

[0043] (5) Cell adhesion molecule signaling pathway. In the inflammatory response, cell adhesion is a key step, which promotes the occurrence of inflammatory response by mediating the interaction between immune cells and damaged tissues. Inhibiting the cell adhesion molecule signaling pathway can block the intracellular immune response mediated by it, reduce the adhesion and migration of leukocytes and endothelial cells, and the infiltration of inflammatory cells, thereby reducing vascular damage and inflammatory response. Compared with the blank group, the cell adhesion molecule signaling pathway was upregulated in the model group, promoting the occurrence of inflammation. After adding essential oils, this pathway was inhibited, which may slow down the inflammatory effect. Thujone, chamomile and eucalyptol had no significant effect on this pathway.

[0044] In summary, compared to the blank control group, multiple signaling pathways and functions that promote inflammation were activated or upregulated in the model group, demonstrating the successful establishment of a vascular inflammation model. The addition of essential oils inhibited these pathways and functions, demonstrating that essential oils can not only be used to prepare drugs that regulate these pathways, with anti-inflammatory and wound-healing effects, but also possess even more desirable anti-inflammatory and wound-healing effects due to their ability to simultaneously regulate multiple pathways.

[0045] 2. Regulatory effects on hypertrophic scar signaling pathways

[0046] HDF cells (human skin dermal fibroblasts) were stimulated with 10 ng / mL TGF-β1 for 48 hours to induce a scar model. Specifically:

[0047] HDF cells were plated for 24 hours, starved for 24 hours, and then treated with medium containing the test drug and 10 ng / mL TGF-β1 for 48 hours before collection. The cells were cultured in HDF-specific medium (purchased from Wuhan Punosai Life Science Co., Ltd.). The remaining treatments were the same as in step 1. The regulatory effects of each drug on the signaling pathway are shown in Table 3.

[0048] Table 3 Comparison of the effects of different drugs on pathway regulation

[0049]

[0050] The results showed that although essential oils contain components such as thujone, chamomilla, and eucalyptol, and these components account for a large proportion of the essential oils, the essential oils still exhibit regulatory effects on some signaling pathways that are different from those of these individual components, indicating that the multiple components within the essential oils cooperate with each other and may play new and different roles. The specific analysis is as follows:

[0051] (1) TGF-β signaling pathway. TGF-β is a multifunctional growth factor that plays a variety of roles in wound healing by regulating cell proliferation, migration, differentiation, ECM production and immune regulation. In the late stage of wound healing, when scar formation becomes a major problem, moderate inhibition of the TGF-β signaling pathway helps reduce scar formation. Compared with the blank group, the TGF-β signaling pathway in the model group was activated, proving that the scar model was successfully constructed. After adding essential oils, the TGF-β signaling pathway was inhibited, which can reduce scar formation. Thujone and chamomile had no significant effect on this pathway. After adding eucalyptol, the pathway was further upregulated, which may promote wound healing to a certain extent, but may also lead to scar formation.

[0052] (2) Cell cycle signaling pathway. Scar formation involves the proliferation, migration and deposition of extracellular matrix (ECM) of fibroblasts. The proliferation of fibroblasts is one of the key steps in scar formation, and the cell cycle signaling pathway regulates the cell proliferation process. If the cell cycle signaling pathway is inhibited, the proliferation of fibroblasts may be hindered, thereby reducing the number of fibroblasts in scar tissue, which to a certain extent helps to alleviate scar formation. Compared with the blank group, the TGF-β signaling pathway in the model group was activated. After adding essential oils and eucalyptol, the cell cycle signaling pathway was inhibited, which may be able to reduce scar formation. Thujone and matricaria had no significant effect on this pathway.

[0053] In summary, compared to the blank control group, multiple signaling pathways and functions that promote scar formation were activated or upregulated in the model group, demonstrating the successful establishment of the scar model. The addition of essential oils inhibited these pathways and functions, demonstrating that essential oils can not only be used to prepare drugs that regulate these pathways and thus reduce scarring, but also have a more ideal scarring effect due to their ability to simultaneously regulate multiple pathways.

[0054] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various deformations, modifications, and substitutions made to the technical solutions of the present invention by ordinary technicians in this field should fall within the scope of protection determined by the claims of the present invention.

Claims

1. An application of an Artemisia essential oil in the preparation of a wound healing promoting and / or anti-inflammatory drug, characterized in that: The components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia essential oil is used in the preparation of a drug for downregulating or inhibiting a TNF signaling pathway.

2. Use of an Artemisia essential oil in the preparation of a wound healing promoting and / or anti-inflammatory drug, characterized in that: The components of the Artemisia plant essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia plant essential oil is used in the preparation of a drug for downregulating or inhibiting the interaction between cytokines and cytokine receptors.

3. Use of an Artemisia essential oil in the preparation of a wound healing promoting and / or anti-inflammatory drug, characterized in that: The components of the Artemisia plant essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia plant essential oil is used in the preparation of a drug for downregulating or inhibiting a NOD-like receptor signaling pathway.

4. Use of an Artemisia essential oil in the preparation of a wound healing promoting and / or anti-inflammatory drug, characterized in that: The components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia essential oil is used in the preparation of a drug for downregulating or inhibiting a Toll-like receptor signaling pathway.

5. Use of an Artemisia essential oil in the preparation of a wound healing promoting and / or anti-inflammatory drug, characterized in that: The components of the Artemisia plant essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia plant essential oil is used in the preparation of a drug for downregulating or inhibiting a cell adhesion molecule signaling pathway.

6. Use of an Artemisia essential oil in the preparation of a medicament for reducing or preventing the formation of hypertrophic scars, characterized in that: The components of the Artemisia plant essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia plant essential oil is used in the preparation of a drug for downregulating or inhibiting the TGF-β signaling pathway.

7. Use of an Artemisia essential oil in the preparation of a medicament for reducing or preventing the formation of hypertrophic scars, characterized in that: The components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogeran and epoxidized humulene II. The Artemisia essential oil is used in the preparation of a drug for downregulating or inhibiting a cell cycle signaling pathway.

8. A drug capable of promoting wound healing, characterized in that: The medicine contains Artemisia essential oil, and the components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogerene and epoxidized humulene II.

9. A drug with anti-inflammatory effect, characterized in that: The medicine contains Artemisia essential oil, and the components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogerene and epoxidized humulene II.

10. A drug capable of reducing or preventing the formation of hypertrophic scars, characterized in that: The medicine contains Artemisia essential oil, and the components of the Artemisia essential oil include: eucalyptol, α-thujone, β-caryophyllene, matricaria, L-borneol, bicyclogerene and epoxidized humulene II.

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