Soybean isolate protein powder production method based on membrane separation technology

Abstract

The invention discloses a soybean protein isolate powder production method based on a membrane separation technology, and relates to the technical field of health food, and the method comprises the following steps: crushing soybean meal powder, carrying out enzymolysis, carrying out concentration and separation through an ultrafiltration membrane system to obtain a concentrated protein solution, and carrying out decoloration, deodorization and desalination treatment to obtain a refined protein solution. And finally, adding nutritional ingredients such as fructo-oligosaccharide, galactooligosaccharide, vitamin C, vitamin E and zinc, uniformly mixing, and spray-drying to obtain powder. By comparing production processes of different embodiments, the influence of production process parameters on the immunomodulatory function of the soybean protein isolate is defined, and the optimal production process is screened out. The soybean protein isolate powder prepared by the method not only retains high nutritional value, but also remarkably improves the immune regulation function, has a wide market prospect, and provides a new growth point for the development of healthy industry.

Description

Technical Field

[0001] The invention relates to the technical field of health food, in particular to a method for producing soybean protein isolate powder based on membrane separation technology. Background Art

[0002] With the increasing emphasis on healthy eating, plant-based protein sources, particularly soy protein isolate, are becoming an important alternative to animal protein due to their high nutritional value, low cholesterol content, and ease of digestion and absorption. Soy protein isolate, a high-quality protein extracted from soybeans, is rich in essential amino acids and exhibits numerous physiological functions, including enhancing immunity and promoting muscle growth. However, soy protein isolates produced using different production processes and conditions can exhibit significant differences in nutritional content, biological activity, and functional properties.

[0003] There are many soy protein isolate products on the market, but the impact of their specific production processes on immunomodulatory effects is insufficiently studied. Traditional production processes can denature proteins due to high-temperature treatment and acid-base adjustments, thereby affecting their biological activity and functional properties. Therefore, developing novel, efficient, and minimally damaging soy protein isolate production processes to enhance their immunomodulatory properties is of great scientific significance and practical value. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the present invention provides a method for producing soy protein isolate powder based on membrane separation technology, which solves the problems raised in the above background technology.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: A method for producing soy protein isolate powder based on membrane separation technology, comprising the following steps:

[0006] S1. Grind soybean meal powder into 80 mesh, mix with water in a ratio of 1:4, adjust the pH value to 7.0, add enzyme preparation for enzymolysis, and perform enzymolysis at 50°C for 2 hours;

[0007] S2. heating the enzymatically hydrolyzed mixture to 80° C. and stirring for 30 minutes to fully dissolve the protein to obtain a protein extract;

[0008] S3, concentrating and separating the protein extract through an ultrafiltration membrane system with a molecular weight cut-off of 10,000 to obtain a concentrated protein solution;

[0009] S4, decolorizing, deodorizing and desalting the concentrated protein solution to obtain a refined protein solution;

[0010] S5. Add fructooligosaccharide, galacto-oligosaccharide, vitamin C, vitamin E and zinc to the refined protein solution and mix well;

[0011] S6. Spray-drying the evenly mixed protein solution to obtain a soy protein isolate powder product.

[0012] Furthermore, the soy protein isolate powder contains the following components by weight: 40-60 kg of soybean meal powder, 160-240 L of water, 0.3-0.6 kg of enzyme preparation, 3-6 kg of oligofructose, 1-4 kg of oligogalactose, 0.1-0.3 kg of vitamin C, 0.08-0.14 kg of vitamin E, and 0.03-0.07 kg of zinc.

[0013] Furthermore, the soy protein isolate powder contains the following components by weight: 40 kg of soybean meal powder, 160 L of water, 0.3 kg of enzyme preparation, 3 kg of oligofructose, 1 kg of oligogalactose, 0.1 kg of vitamin C, 0.08 kg of vitamin E, and 0.03 kg of zinc.

[0014] Furthermore, the soy protein isolate powder contains the following components by weight: 50 kg of soybean meal powder, 200 L of water, 0.5 kg of enzyme preparation, 5 kg of oligofructose, 3 kg of oligogalactose, 0.2 kg of vitamin C, 0.1 kg of vitamin E, and 0.05 kg of zinc.

[0015] Furthermore, the soy protein isolate powder contains the following components by weight: 60 kg of soybean meal powder, 240 L of water, 0.6 kg of enzyme preparation, 6 kg of oligofructose, 4 kg of oligogalactose, 0.3 kg of vitamin C, 0.14 kg of vitamin E, and 0.07 kg of zinc.

[0016] Furthermore, in step S1, the enzyme preparation is any one of alkaline protease, neutral protease, and composite protease.

[0017] Furthermore, in step S5, 3 kg of astragalus polysaccharide, 2 kg of wolfberry polysaccharide and 0.5 kg of green tea extract were added.

[0018] Furthermore, in step S6, before spray drying, the protein solution is homogenized to improve the uniformity and dispersibility of the protein particles during the spray drying process, thereby improving the solubility and taste of the soy protein isolate powder.

[0019] Furthermore, after step S6, the method further includes quality inspection and packaging of the obtained soy protein isolate powder, wherein the quality inspection includes protein content determination, microbial index detection, and heavy metal and harmful substance residue detection.

[0020] The present invention provides a method for producing soy protein isolate powder based on membrane separation technology. Compared with the existing technology, it has the following advantages:

[0021] 1. By comparing the production processes of different embodiments, it is possible to clarify which factors (such as temperature, pH, extraction time, etc.) have a significant impact on the immunomodulatory function of soy protein isolate, thereby providing a scientific basis for optimizing the production process.

[0022] 2. Screening out the soy protein isolate production process with the best immune regulation function can significantly improve the added value and market competitiveness of the product and meet consumers' demand for healthy, nutritious and functional foods.

[0023] 3. The present invention not only provides experimental evidence for the immunomodulatory function of soy protein isolate, but also lays the foundation for further in-depth research on the biological activity, functional properties and mechanism of action of soy protein isolate, and helps to promote the development of scientific research in related fields.

[0024] 4. With people's increasing attention to healthy diet, soy protein isolate products with immune regulation function will have broad market prospects. This invention will provide a new growth point for the development of the health industry and promote the extension and expansion of the related industrial chain. DETAILED DESCRIPTION

[0025] Hereinafter, the technical solutions of the present invention will be described in detail through specific embodiments. However, it should be clearly stated that these embodiments are provided for illustration only and are not to be construed as limiting the scope of the present invention.

[0026] Example 1

[0027] A method for producing soy protein isolate powder based on membrane separation technology comprises the following steps:

[0028] S1. Grind soybean meal powder into 80 mesh, weigh 40 kg of soybean meal powder and mix with 160 L of water, adjust the pH value to 7.0, add 0.3 kg of enzyme preparation for enzymolysis (the enzyme preparation is any one of alkaline protease, neutral protease, and composite protease), the enzymolysis temperature is 50 ° C, and the enzymolysis time is 2 hours;

[0029] S2. heating the enzymatically hydrolyzed mixture to 80° C. and stirring for 30 minutes to fully dissolve the protein to obtain a protein extract;

[0030] S3, concentrating and separating the protein extract through an ultrafiltration membrane system with a molecular weight cut-off of 10,000 to obtain a concentrated protein solution;

[0031] S4, decolorizing, deodorizing and desalting the concentrated protein solution to obtain a refined protein solution;

[0032] S5. Add 3 kg of fructooligosaccharide, 1 kg of galacto-oligosaccharide, 0.1 kg of vitamin C, 0.08 kg of vitamin E, 0.03 kg of zinc, 3 kg of astragalus polysaccharide, 2 kg of wolfberry polysaccharide and 0.5 kg of green tea extract to the refined protein solution and mix well.

[0033] S6, homogenizing the protein solution to improve the uniformity and dispersibility of the protein particles during the spray drying process, thereby improving the solubility and taste of the soy protein isolate powder, and spray drying the uniformly mixed protein solution to obtain a soy protein isolate powder product;

[0034] S7. The obtained soy protein isolate powder is subjected to quality inspection and packaging steps, wherein the quality inspection includes protein content determination, microbial index detection, heavy metal and harmful substance residue detection, to ensure that the product meets food safety standards and customer requirements.

[0035] Example 2

[0036] A method for producing soy protein isolate powder based on membrane separation technology comprises the following steps:

[0037] S1. Grind soybean meal into 80 mesh, weigh 50 kg of soybean meal and mix with 200 L of water, adjust the pH to 7.0, add 0.5 kg of enzyme preparation for enzymolysis (the enzyme preparation is any one of alkaline protease, neutral protease, and composite protease), the enzymolysis temperature is 50 ° C, and the enzymolysis time is 2 hours;

[0038] S2. heating the enzymatically hydrolyzed mixture to 80° C. and stirring for 30 minutes to fully dissolve the protein to obtain a protein extract;

[0039] S3, concentrating and separating the protein extract through an ultrafiltration membrane system with a molecular weight cut-off of 10,000 to obtain a concentrated protein solution;

[0040] S4, decolorizing, deodorizing and desalting the concentrated protein solution to obtain a refined protein solution;

[0041] S5. Add 5 kg of fructooligosaccharide, 3 kg of galacto-oligosaccharide, 0.2 kg of vitamin C, 0.1 kg of vitamin E, 0.05 kg of zinc, 3 kg of astragalus polysaccharide, 2 kg of wolfberry polysaccharide and 0.5 kg of green tea extract to the refined protein solution and mix well.

[0042] S6, homogenizing the protein solution to improve the uniformity and dispersibility of the protein particles during the spray drying process, thereby improving the solubility and taste of the soy protein isolate powder, and spray drying the uniformly mixed protein solution to obtain a soy protein isolate powder product;

[0043] S7. The obtained soy protein isolate powder is subjected to quality inspection and packaging steps, wherein the quality inspection includes protein content determination, microbial index detection, heavy metal and harmful substance residue detection, to ensure that the product meets food safety standards and customer requirements.

[0044] Example 3

[0045] A method for producing soy protein isolate powder based on membrane separation technology comprises the following steps:

[0046] S1. Grind soybean meal powder into 80 mesh, weigh 60 kg of soybean meal powder and mix with 240 L of water, adjust the pH value to 7.0, add 0.6 kg of enzyme preparation for enzymolysis (the enzyme preparation is any one of alkaline protease, neutral protease, and composite protease), the enzymolysis temperature is 50 ° C, and the enzymolysis time is 2 hours;

[0047] S2. heating the enzymatically hydrolyzed mixture to 80° C. and stirring for 30 minutes to fully dissolve the protein to obtain a protein extract;

[0048] S3, concentrating and separating the protein extract through an ultrafiltration membrane system with a molecular weight cut-off of 10,000 to obtain a concentrated protein solution;

[0049] S4, decolorizing, deodorizing and desalting the concentrated protein solution to obtain a refined protein solution;

[0050] S5. Add 6 kg of fructooligosaccharide, 4 kg of galacto-oligosaccharide, 0.3 kg of vitamin C, 0.14 kg of vitamin E, 0.07 kg of zinc, 3 kg of astragalus polysaccharide, 2 kg of wolfberry polysaccharide and 0.5 kg of green tea extract to the refined protein solution and mix well.

[0051] S6, homogenizing the protein solution to improve the uniformity and dispersibility of the protein particles during the spray drying process, thereby improving the solubility and taste of the soy protein isolate powder, and spray drying the uniformly mixed protein solution to obtain a soy protein isolate powder product;

[0052] S7. The obtained soy protein isolate powder is subjected to quality inspection and packaging steps, wherein the quality inspection includes protein content determination, microbial index detection, heavy metal and harmful substance residue detection, to ensure that the product meets food safety standards and customer requirements.

[0053] Experimental design

[0054] 1. Experimental purpose: To evaluate the effects of soy protein isolate powder produced in different embodiments on the immunity of mice.

[0055] 2. Experimental animals: A total of 160 Kunming mice were randomly divided into 4 groups, with 40 mice in each group.

[0056] 3. Experimental groups: control group, Example 1 group, Example 2 group, and Example 3 group.

[0057] 4. Feed preparation: The control group received conventional feed; the experimental group added the soy protein isolate powder produced in each example to the conventional feed.

[0058] 5. Feeding cycle: 4 weeks.

[0059] 6. Immunity assessment indicators: splenic lymphocyte proliferation response ability, natural killer (NK) cell activity, serum hemolysin level, and the number of antibody-producing cells.

[0060] A. Proliferative response of spleen lymphocytes

[0061] Group OD value (optical density, reflecting the degree of lymphocyte proliferation) control group 0.25±0.05 Example 1 group 0.35±0.06* Example 2 group 0.38±0.07** Example 3 group 0.32±0.05*

[0062] Note: * indicates P < 0.05 compared with the control group; ** indicates P < 0.01 compared with the control group

[0063] B. Natural killer (NK) cell activity

[0064] Group NK cell activity (%) control group 45±5 Example 1 group 55±6* Example 2 group 60±7** Example 3 group 52±5*

[0065] Note: * indicates P < 0.05 compared with the control group; ** indicates P < 0.01 compared with the control group

[0066] C. Serum hemolysin level

[0067] Group Antibody count (reflects serum hemolysin level) control group 120±20 Example 1 group 135±25 Example 2 group 150±30** Example 3 group 130±20

[0068] Note: * indicates P < 0.05 compared with the control group; ** indicates P < 0.01 compared with the control group

[0069] D. Number of antibody-producing cells

[0070] Group Number of hemolytic plaques (×106 spleen cells) control group 1.2±0.3 Example 1 group 1.6±0.4* Example 2 group 1.9±0.5** Example 3 group 1.5±0.3*

[0071] Note: * indicates P < 0.05 compared with the control group; ** indicates P < 0.01 compared with the control group

[0072] 7. Data Analysis and Conclusion

[0073] 1. According to the above data, the Example 2 group showed the most significant improvement in spleen lymphocyte proliferation response ability, NK cell activity, serum hemolysin level and the number of antibody-producing cells, which were statistically significantly different from the control group (P<0.01);

[0074] II, Example 1 and Example 3 groups also improved the immunity of mice to a certain extent, but compared with the control group, the difference only reached the statistically significant level (P < 0.05);

[0075] Therefore, it can be inferred that the soy protein isolate powder produced in Example 2 performs best in enhancing the immunity of mice.

Claims

1. A method for producing soy protein isolate powder based on membrane separation technology, characterized in that: The steps include: S1. Grind soybean meal powder into 80 mesh, mix with water in a ratio of 1:4, adjust the pH value to 7.0, add enzyme preparation for enzymolysis, and perform enzymolysis at 50°C for 2 hours; S2. heating the enzymatically hydrolyzed mixture to 80° C. and stirring for 30 minutes to fully dissolve the protein to obtain a protein extract; S3, concentrating and separating the protein extract through an ultrafiltration membrane system with a molecular weight cut-off of 10,000 to obtain a concentrated protein solution; S4, decolorizing, deodorizing and desalting the concentrated protein solution to obtain a refined protein solution; S5. Add fructooligosaccharide, galacto-oligosaccharide, vitamin C, vitamin E and zinc to the refined protein solution and mix well; S6. Spray-drying the evenly mixed protein solution to obtain a soy protein isolate powder product.

2. A method for producing soy protein isolate powder based on membrane separation technology according to claim 1, characterized in that, The soy protein isolate powder contains the following components by weight: 40-60 kg of soybean meal powder, 160-240 L of water, 0.3-0.6 kg of enzyme preparation, 3-6 kg of oligofructose, 1-4 kg of oligogalactose, 0.1-0.3 kg of vitamin C, 0.08-0.14 kg of vitamin E, and 0.03-0.07 kg of zinc.

3. A method for producing soy protein isolate powder based on membrane separation technology according to claim 2, characterized in that, The soy protein isolate powder contains the following components by weight: 40 kg of soybean meal powder, 160 L of water, 0.3 kg of enzyme preparation, 3 kg of oligofructose, 1 kg of oligogalactose, 0.1 kg of vitamin C, 0.08 kg of vitamin E, and 0.03 kg of zinc.

4. A method for producing soy protein isolate powder based on membrane separation technology according to claim 2, characterized in that, The soy protein isolate powder contains the following components by weight: 50 kg of soybean meal powder, 200 L of water, 0.5 kg of enzyme preparation, 5 kg of oligofructose, 3 kg of oligogalactose, 0.2 kg of vitamin C, 0.1 kg of vitamin E, and 0.05 kg of zinc.

5. A method for producing soy protein isolate powder based on membrane separation technology according to claim 2, characterized in that, The soy protein isolate powder contains the following components by weight: 60 kg of soybean meal powder, 240 L of water, 0.6 kg of enzyme preparation, 6 kg of oligofructose, 4 kg of oligogalactose, 0.3 kg of vitamin C, 0.14 kg of vitamin E, and 0.07 kg of zinc.

6. A method for producing soy protein isolate powder based on membrane separation technology according to any one of claims 1 to 5, characterized in that: In step S1, the enzyme preparation is any one of alkaline protease, neutral protease, and composite protease.

7. The method for producing soy protein isolate powder based on membrane separation technology according to any one of claims 1 to 5, characterized in that: In step S5, 3 kg of astragalus polysaccharide, 2 kg of wolfberry polysaccharide and 0.5 kg of green tea extract were further added.

8. The method for producing soy protein isolate powder based on membrane separation technology according to any one of claims 1 to 5, characterized in that: In step S6, before spray drying, the protein solution is further homogenized to improve the uniformity and dispersibility of the protein particles during the spray drying process, thereby improving the solubility and taste of the soy protein isolate powder.

9. The method for producing soy protein isolate powder based on membrane separation technology according to any one of claims 1 to 5, characterized in that: After step S6, the process further includes quality inspection and packaging of the obtained soy protein isolate powder, wherein the quality inspection includes protein content determination, microbial index detection, and heavy metal and harmful substance residue detection.