Osteochondral graft centrifugal device

Abstract

The osteochondral graft centrifugal device comprises a centrifugal tube and a separation assembly located in the centrifugal tube, the separation assembly comprises a cylindrical first support and a cylindrical second support, the outer diameter of the first support is matched with the inner diameter of the centrifugal tube, and the second support is far away from the open end of the centrifugal tube relative to the first support and is connected with the first support; the outer diameter of the second support is smaller than the inner diameter of the first support, an annular gap is formed between the second support and the inner wall of the centrifugal tube, a step face is formed in the connecting position of the second support and the first support, and a first through notch is formed in the step face. According to the osteochondral graft centrifugal device, the separating assembly is arranged in the centrifugal tube to bear the osteochondral graft to be treated, the articular cartilage and bone tissue below the articular cartilage can be protected, and meanwhile the purpose of separating bone marrow in the osteochondral graft to be treated is achieved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of osteochondral allotransplantation, and in particular to an osteochondral transplant centrifugal device. Background Art

[0002] Osteochondral allograft transplantation has a long history of clinical application in repairing osteochondral defects, with a 10-year graft survival rate exceeding 80%. Key to surgical success is the presence of well-active cartilage tissue and structurally stable subchondral bone in the graft. The subchondral bone portion of the graft contains bone marrow tissue, a vascular network, and blood components, including a variety of living cells such as hematopoietic cells, leukocytes, and mesenchymal cells. These cells carry alloantigens and are highly immunogenic, potentially triggering immune rejection. Therefore, prior to transplantation, the bone marrow components of the subchondral bone should be removed as much as possible to reduce the risk of immune rejection.

[0003] However, when handling osteochondral grafts, care must be taken to protect the articular cartilage and the underlying bone tissue from damage. Therefore, some chemical treatments that may damage cartilage or bone tissue (such as strong acid and strong alkaline solutions) are not suitable for clinical use. Summary of the Invention

[0004] In order to solve the problems existing in the prior art, the present disclosure provides the following technical solutions: The present disclosure provides an osteochondral transplant centrifugal device, comprising a centrifugal tube and a separation component located in the centrifugal tube, wherein the separation component comprises: a first bracket, wherein the first bracket is configured to be cylindrical, and an outer diameter of the first bracket is configured to match an inner diameter of the centrifuge tube; The second bracket is cylindrical and is configured to be connected to the first bracket. The second bracket is farther away from the open end of the centrifuge tube relative to the first bracket. The outer diameter of the second bracket is smaller than the inner diameter of the first bracket, and an annular gap is formed between the second bracket and the inner wall of the centrifuge tube. A step surface is formed at the position where the second bracket is connected to the first bracket, and a first notch is provided on the step surface.

[0005] In one embodiment of the present disclosure, a flange extending outward is provided on one side of the first bracket close to the mouth of the centrifuge tube. The outer diameter of the flange is larger than the inner diameter of the mouth of the centrifuge tube and is constructed to be supported on the mouth of the centrifuge tube.

[0006] In one embodiment of the present disclosure, the centrifuge tube further comprises a cover body matched with the tube mouth end, the outer diameter of the flange is adapted to the inner diameter of the cover body, and the flange is constructed to cooperate with the cover body to complete the seal.

[0007] In one embodiment of the present disclosure, the inner cavity of the first bracket is connected to the inner cavity of the second bracket, and a supporting platform is provided at the bottom end of the second bracket, and a plurality of through-holes are provided on the supporting platform.

[0008] In one embodiment of the present disclosure, the supporting platform is configured to be cross-shaped.

[0009] In another embodiment of the present disclosure, the centrifuge tube includes a tube body and a tapered portion located at the lower end of the tube body; the outer wall of the bottom of the second bracket is constructed to have a plurality of outwardly extending fixing portions spaced circumferentially, the outer edge of the fixing portion is constructed to match the inner diameter of the tube body, and the second bracket is constructed to be supported on the tapered portion.

[0010] In another embodiment of the present disclosure, a second notch is formed between two adjacent fixing portions, and the second notch is configured to pass through both ends of the fixing portion.

[0011] In one embodiment of the present disclosure, a bearing platform is provided at the bottom of the first bracket, and a plurality of through-openings are provided on the bearing platform.

[0012] In one embodiment of the present disclosure, the bottom of the second bracket is configured to have an open opening.

[0013] In one embodiment of the present disclosure, a plurality of first openings are evenly spaced along the circumferential direction on the side wall of the first bracket, a plurality of second openings are evenly spaced along the circumferential direction on the side wall of the second bracket, and the first openings and the second openings are staggered with each other.

[0014] The disclosed osteochondral graft centrifuge device comprises a separation assembly disposed within a centrifuge tube to hold the osteochondral graft to be processed. The separation assembly is divided into a first bracket and a second bracket. The outer wall of the first bracket is aligned with the inner wall of the centrifuge tube, effectively preventing the separation assembly from vibrating during centrifugation. An annular gap is provided between the second bracket and the inner wall of the centrifuge tube, allowing the centrifugally separated material to flow naturally to the bottom of the centrifuge tube. The disclosed osteochondral graft centrifuge device can separate bone marrow while protecting the articular cartilage and the underlying bone tissue.

[0015] Other features and advantages of the present disclosure will become apparent from the following detailed description of exemplary embodiments of the present disclosure with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the present disclosure and, together with the description, serve to explain the principles of the present disclosure.

[0017] Figure 1 1 is a schematic structural diagram of an osteochondral transplant centrifugal device provided in one embodiment of the present disclosure; Figure 2 is a structural diagram of a separation assembly provided in one embodiment of the present disclosure; Figure 3 is a schematic structural diagram of an osteochondral transplant centrifugal device provided by another embodiment of the present disclosure; Figure 4 It is a structural schematic diagram of a separation component provided by another embodiment of the present disclosure.

[0018] Figures 1 to 4 The one-to-one correspondence between the component names and the reference numerals is as follows: 1. Centrifuge tube; 11. Tube body; 12. Conical portion; 2. Separation assembly; 21. First bracket; 211. Flange; 212. First opening; 22. Second bracket; 221. Fixing portion; 222. Second notch; 223. Second opening; 23. Step surface; 231. First notch; 3. Cover; 4. Support platform; 41. Through-portion; 5. Opening. DETAILED DESCRIPTION

[0019] Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. It should be noted that unless otherwise specifically stated, the relative arrangement of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present disclosure.

[0020] The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the present disclosure, its application, or uses.

[0021] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered part of the specification.

[0022] It should be noted that like reference numerals and letters refer to like items in the following figures, and therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0023] In this document, “upper”, “lower”, “front”, “back”, “left”, “right”, etc. are only used to indicate the relative position relationship between related parts, rather than to limit the absolute positions of these related parts.

[0024] In this article, "first", "second", etc. are only used to distinguish each other, and do not indicate the importance and order, or the prerequisite for each other's existence.

[0025] In this document, “equal”, “same”, etc. are not strictly limited in a mathematical and / or geometric sense, but also include errors that can be understood by those skilled in the art and are allowed in manufacturing or use.

[0026] The present disclosure provides a centrifugal device for bone cartilage transplantation, comprising a centrifuge tube and a separation component located in the centrifuge tube, wherein the tube body of the centrifuge tube is cylindrical, and a first cylindrical bracket and a second cylindrical bracket are arranged in the centrifuge tube, wherein the outer diameter of the first bracket is adapted to the inner diameter of the centrifuge tube, that is, the outer wall of the first bracket can be tightly attached to the inner wall of the centrifuge tube 1, thereby preventing the separation component from shaking during the centrifugation process, thereby reducing the centrifugal effect. The second bracket is away from the open end of the centrifuge tube relative to the first bracket and is connected to the first bracket, and the outer diameter of the second bracket is smaller than the inner diameter of the first bracket, so that an annular gap is formed between the second bracket and the inner wall of the centrifuge tube, and the first notch extends inwardly relative to the outer wall of the first bracket to a position close to the outer wall of the second bracket. The first notch and the annular gap can provide a channel for the separated bone marrow components to flow downward into the bottom of the centrifuge tube during the centrifugation process, thereby allowing the separated bone marrow components to flow smoothly into the bottom of the centrifuge tube.

[0027] The disclosed osteochondral graft centrifuge device incorporates a separation assembly within a centrifuge tube to hold the osteochondral graft to be separated. The separation assembly is divided into a first bracket and a second bracket. The outer wall of the first bracket is aligned with the inner wall of the centrifuge tube, effectively preventing the separation assembly from vibrating during centrifugation. An annular gap is provided between the second bracket and the inner wall of the centrifuge tube, allowing the centrifugally separated material to flow naturally to the bottom of the centrifuge tube. The disclosed osteochondral graft centrifuge device can separate bone marrow while protecting the articular cartilage and the underlying bone tissue.

[0028] For ease of understanding, refer to Figures 1 to 4 , the specific structure and working principle of the osteochondral transplant centrifugal device disclosed in the present invention are explained in detail in combination with two embodiments.

[0029] Reference Figures 1 to 4 The present disclosure provides a bone cartilage transplant centrifugal device, including a centrifuge tube 1 and a separation component 2 located in the centrifuge tube 1, the separation component 2 includes a first cylindrical bracket 21 and a second bracket 22, the outer diameter of the first bracket 21 is adapted to the inner diameter of the centrifuge tube 1, the second bracket 22 is connected to the first bracket 21, the second bracket 22 is away from the open end of the centrifuge tube 1 relative to the first bracket 21, the outer diameter of the second bracket 22 is smaller than the inner diameter of the first bracket 21, and an annular gap is formed between the second bracket 22 and the inner wall of the centrifuge tube 1, a step surface 23 is formed at the position where the second bracket 22 is connected to the first bracket 21, and a first through-notch 231 is provided on the step surface 23.

[0030] Specifically, since the centrifuge tube 1 has a cylindrical body 11, the first bracket 21 and the second bracket 22 placed therein are also cylindrical to match the shape of the centrifuge tube 1. Furthermore, the outer diameter of the first bracket 21 matches the inner diameter of the centrifuge tube 1, so that the outer wall of the first bracket 21 fits snugly against the inner wall of the centrifuge tube 1, thereby preventing the first bracket 21 from shaking relative to the centrifuge tube 1 during centrifugation. The second bracket 22 is located at a position away from the tube mouth of the centrifuge tube 1 relative to the first bracket 21. The first bracket 21 and the second bracket 22 are fixedly connected, and a step surface 23 is formed at the connection position. A plurality of penetrating first notches 231 are arranged at intervals on the step surface 23. Since the outer diameter of the second bracket 22 is smaller than the inner diameter of the first bracket 21, an annular gap is formed between the second bracket 22 and the inner wall of the centrifuge tube 1. The first notch 231 extends inward relative to the outer wall of the first bracket 21 to a position close to the outer wall of the second bracket 22. The first notch 231 and the annular gap can provide a channel for the separated bone marrow components to flow downward into the bottom of the centrifuge tube 1 during the centrifugation process, thereby allowing the separated bone marrow components to flow smoothly into the bottom of the centrifuge tube 1.

[0031] The osteochondral centrifuge device disclosed herein can efficiently separate or remove bone marrow components from osteochondral grafts without compromising chondrocyte activity. The centrifuge device comprises a centrifuge tube 1 and a separation assembly 2 that can be placed within the centrifuge tube 1. The separation assembly 2 can be adapted to fit into centrifuge tubes 1 of varying sizes. By placing the osteochondral graft within the separation assembly 2 during centrifugation, the bone marrow components can be effectively separated by centrifugation.

[0032] The separation component 2 in the bone cartilage centrifuge device disclosed herein has two forms, one is a suspended separation component 2, and the other is a floor-standing separation component 2. The floor-standing separation component 2 can be adapted to various types of centrifuge tubes 1, and the suspended separation component 2 needs to be used in conjunction with a centrifuge tube 1 with a cover 3.

[0033] Example 1 The bone cartilage centrifugal device of this embodiment is provided with a suspension type separation component 2, such as Figure 1 and Figure 2 shown.

[0034] refer to Figure 1 and Figure 2 In one embodiment of the present disclosure, a flange 211 extending outward is provided on one side of the first bracket 21 close to the mouth of the centrifuge tube 1. The outer diameter of the flange 211 is larger than the inner diameter of the mouth of the centrifuge tube 1 and is constructed to be supported on the mouth of the centrifuge tube 1.

[0035] Specifically, the sidewall of the first bracket 21 fits snugly against the inner wall of the centrifuge tube 1, effectively preventing the separation assembly 2 from shaking during centrifugation. An outwardly extending flange 211 is added to the outer wall of the first bracket 21 near the tube end. The outer diameter of the flange 211 is larger than the inner diameter of the tube end, allowing the first bracket 21 to be stably suspended on the tube end of the centrifuge tube 1. This ensures that the separation assembly 2 will not easily slip or shift during use, thereby further enhancing the stability of the separation assembly 2. Furthermore, this makes installation and removal much simpler and faster, without the need for complex tools or fixtures.

[0036] refer to Figure 1 and Figure 2 The centrifuge tube 1 also includes a cover body 3 that matches the tube end. The outer diameter of the flange 211 is adapted to the inner diameter of the cover body 3. The flange 211 is constructed to match the cover body 3 to complete the seal.

[0037] Specifically, in order to ensure the sealing of the centrifugation process, the outer diameter of the flange 211 is adapted to the inner diameter of the cover body 3, so that the end face and side face of the flange 211 are in contact with and fit with the inner surface of the cover body 3, ensuring that when the cover body 3 covers the tube end, there is no gap between the flange 211 and the cover body 3, thereby effectively preventing external impurities from entering the centrifuge tube 1 during use, and also preventing the internal osteochondral graft or bone marrow components from escaping from the centrifuge tube 1 and being contaminated.

[0038] In a specific embodiment of the present disclosure, referring to Figure 1 and Figure 2 The inner cavity of the first bracket 21 is connected to the inner cavity of the second bracket 22. A supporting platform 4 is provided at the bottom end of the second bracket 22. The supporting platform 4 is provided with a plurality of through-holes 41.

[0039] Specifically, the first bracket 21 and the second bracket 22 are both cylindrical structures. The two are coaxially arranged and connected to each other, and their internal cavities are interconnected. The outer diameter of the first bracket 21 is the same as the inner diameter of the centrifuge tube 1 and is larger than the outer diameter of the second bracket 22. Therefore, when the second bracket 22 is connected to the first bracket 21, it is at least partially arranged on the bottom edge of the first bracket 21. In the suspended separation component 2, the second bracket 22 serves as the main load-bearing component, and a load-bearing platform 4 is provided at its bottom end. The load-bearing platform 4 is horizontally arranged at the bottom of the second bracket 22 for placing the bone cartilage graft to be separated. Since the load-bearing platform 4 is stably connected to the second bracket 22, the second bracket 22 is fixedly connected to the first bracket 21. Therefore, when the first bracket 21 is stably connected to the centrifuge tube 1, the load-bearing platform 4 on the second bracket 22 can also remain stable during high-speed rotation, thereby effectively preventing the bone cartilage graft to be processed from being displaced relative to the centrifuge tube 1.

[0040] Furthermore, a plurality of through openings 41 are provided on the supporting platform 4. These through openings 41 are mainly used for separating the bone marrow components from the tissue under the centrifugal force of the osteochondral graft during the centrifugation process, and smoothly discharging them through the through openings 41 and into the bottom of the centrifuge tube 1, thereby realizing efficient separation and collection of the target components.

[0041] As mentioned above, the separation component 2 is intended to provide stable support for the osteochondral graft to be processed, while allowing the liquid components to be discharged smoothly without affecting the position and integrity of the solid tissue, and can achieve effective separation of bone marrow without causing mechanical damage to the cartilage and bone tissue.

[0042] Example 2 The bone cartilage centrifugal device of this embodiment is provided with a floor-standing separation component 2, such as Figure 3 and Figure 4 shown.

[0043] refer to Figure 3 and Figure 4 In another embodiment of the present disclosure, the centrifuge tube 1 includes a tube body 11 and a tapered portion 12 located at the lower end of the tube body 11; the outer wall of the bottom of the second bracket 22 is constructed to have a plurality of outwardly extending fixing portions 221 spaced apart along the circumferential direction, the outer edge of the fixing portion 221 is constructed to adapt to the inner diameter of the tube body 11, and the second bracket 22 is constructed to be supported on the tapered portion 12.

[0044] Specifically, the centrifuge tube 1 comprises a cylindrical tube body 11 and a tapered portion 12 at the lower end of the tube body 11. A floor-type separation assembly 2 is employed in the centrifuge device. To further prevent vibration during centrifugation, the floor-type separation assembly 2, in addition to ensuring that the sidewalls of the first bracket 21 abut against the inner wall of the centrifuge tube 1, further comprises a plurality of outwardly extending fixing portions 221 spaced circumferentially on the outer wall of the bottom of the second bracket 22. The ends of the fixing portions 221 form contact surfaces of a certain width, which are capable of achieving stable contact with the inner wall of the centrifuge tube 1. In particular, the fixing portions 221 are positioned at the contact area where the tube body 11 and the tapered portion 12 meet, i.e., above the tapered portion 12. This is because the diameter of the tube body 11 gradually decreases as it transitions to the tapered portion 12, forming a region with high structural strength and strong support stability. Furthermore, the outer diameter of the fixing portions 221 is larger than the inner diameter of the tapered portion, which can provide a certain degree of support for the separation assembly 2, thereby further improving the stability of the separation assembly 2.

[0045] Furthermore, multiple fixing portions 221 are provided, which can make multi-point contact with the inner wall of the centrifuge tube 1, so that the second bracket 22 receives more uniform support force during rotation, effectively suppressing vibration or shaking caused by center of gravity offset or uneven centrifugal force.

[0046] refer to Figure 3 and Figure 4 In another embodiment of the present disclosure, a second notch 222 is formed between two adjacent fixing portions 221 , and the second notch 222 is configured to pass through both ends of the fixing portion 221 .

[0047] Specifically, multiple fixing portions 221 are arranged at intervals along the circumferential direction, and multiple second gaps 222 are formed between two adjacent fixing portions 221. In addition to increasing the discharge path of the fluid and thus more efficiently separating the bone marrow components, the presence of the second gaps 222 also helps to reduce the overall weight of the second bracket 22, thereby reducing the load of the centrifuge.

[0048] refer to Figure 3 and Figure 4 In another embodiment of the present disclosure, a supporting platform 4 is provided at the bottom of the first bracket 21 , and a plurality of through-holes 41 are provided on the supporting platform 4 .

[0049] Specifically, in the floor-standing separation assembly 2, a support platform 4 is provided at the bottom of the first support 21. This support platform 4 is located between the first support 21 and the second support 22, providing a stable and flat placement platform for the osteochondral graft to be separated. The support platform 4 is provided with a plurality of through-holes 41 extending through the inner lumens of the first support 21 and the second support 22 and communicating with the inner lumen of the centrifuge tube 1. Under the action of centrifugal force, the bone marrow components separated from the osteochondral graft can be smoothly discharged through these through-holes 41 and enter the collection area below or the bottom of the centrifuge tube 1, achieving effective separation and collection of the target components.

[0050] refer to Figure 3 and Figure 4 In one embodiment of the present disclosure, the bottom of the second bracket 22 is configured to have an open opening 5 .

[0051] Specifically, unlike the suspended separation assembly 2, in the floor-standing separation assembly 2, an open opening 5 is provided at the bottom of the second bracket 22, the main function of which is to provide a discharge channel for the liquid components separated during the centrifugation process, so that these components can flow smoothly into the bottom of the centrifuge tube 1 below, thereby achieving efficient separation.

[0052] refer to Figures 1 to 4 In the two embodiments of the present disclosure, the supporting platform 4 is constructed in a cross shape.

[0053] Specifically, whether in a suspended separation component 2 or a floor-standing separation component 2, the support platform 4 is constructed in a cross shape. The cross-shaped support platform 4 is composed of two cross-distributed support arms, and the overall shape is a "cross". Such a structure maximizes the pore area while carrying the graft, so that the bone marrow components can be more efficiently precipitated from the tissue during the centrifugation process and smoothly discharged through the gaps in the support platform 4, avoiding liquid retention and improving separation efficiency. At the same time, the openness and symmetry of the cross-shaped structure enable it to flexibly adapt to osteochondral grafts of different diameters and shapes, including those with irregular shapes or uneven edges. This flexible bearing method not only improves the versatility of the device, but also enhances stability and safety during use. In addition, the cross-shaped support platform 4 is subjected to uniform force during rotation, which helps to reduce structural imbalance problems caused by eccentricity or vibration, thereby improving the operating stability of the entire separation component 2 under high-speed centrifugation.

[0054] refer to Figures 1 to 4 In one embodiment of the present disclosure, a plurality of first openings 212 are evenly spaced along the circumferential direction on the side wall of the first bracket 21, and a plurality of second openings 223 are evenly spaced along the circumferential direction on the side wall of the second bracket 22, and the first openings 212 and the second openings 223 are staggered with each other.

[0055] Specifically, from the perspective of structural strength, although the present disclosure sets a plurality of first openings 212 and second openings 223 on the side walls of the first bracket 21 and the second bracket 22 respectively, the structural weakening caused by local material loss is effectively avoided by uniformly distributing and staggering the circumference. On the contrary, this design achieves a reasonable dispersion of stress to a certain extent, so that the bracket can still maintain good rigidity and deformation resistance when bearing the centrifugal load brought by high-speed rotation. During high-speed centrifugation, the mass of the separation component 2 itself will directly affect the load of the centrifuge and the dynamic balance of the entire system. The present disclosure reasonably sets the first openings 212 and the second openings 223 on the side walls of the first bracket 21 and the second bracket 22, thereby removing some materials in the non-load-bearing area while ensuring structural strength, thereby significantly reducing the overall weight of the separation component 2.

[0056] Furthermore, since the first opening 212 and the second opening 223 are evenly distributed along the circumference, the symmetry of the separation component 2 can be maintained while reducing the weight, thereby avoiding the center of gravity shift or rotational instability caused by local material loss.

[0057] The bone cartilage graft centrifuge device provided by the present disclosure is configured to carry the bone cartilage graft to be separated by arranging a separation component inside a centrifuge tube. At the same time, the separation component can allow the liquid component to be discharged smoothly without affecting the position and integrity of the solid tissue. The separation component is divided into a first bracket and a second bracket. By making the outer wall of the first bracket fit with the inner wall of the centrifuge tube, the separation component is effectively prevented from shaking during the centrifugation process. By providing an annular gap between the second bracket and the inner wall of the centrifuge tube, the centrifugally separated material can naturally flow into the bottom of the centrifuge tube. Therefore, by selecting an appropriate centrifugal speed and time, the present disclosure can achieve the purpose of separating the internal bone marrow while protecting the articular cartilage and the bone tissue below it.

[0058] The embodiments of the present disclosure have been described above. The above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terms used herein are selected to best explain the principles of the embodiments, their practical applications, or technical improvements in the marketplace, or to enable other persons skilled in the art to understand the embodiments disclosed herein. The scope of the present disclosure is defined by the appended claims.

Claims

1. A centrifugal device for osteochondral graft, characterized in that: It comprises a centrifuge tube (1) and a separation component (2) located in the centrifuge tube (1), wherein the separation component (2) comprises: A first bracket (21), the first bracket (21) is configured to be cylindrical, and the outer diameter of the first bracket (21) is configured to match the inner diameter of the centrifuge tube (1); The second bracket (22) is cylindrical and is configured to be connected to the first bracket (21). The second bracket (22) is farther away from the tube mouth of the centrifuge tube (1) relative to the first bracket (21). The outer diameter of the second bracket (22) is smaller than the inner diameter of the first bracket (21), and an annular gap is formed between the second bracket (22) and the inner wall of the centrifuge tube (1). A step surface (23) is formed at the position where the second bracket (22) is connected to the first bracket (21), and a first notch (231) is provided on the step surface (23).

2. The osteochondral graft centrifugal device according to claim 1, wherein: A flange (211) extending outward is provided on one side of the first bracket (21) close to the mouth of the centrifuge tube (1); the outer diameter of the flange (211) is larger than the inner diameter of the mouth of the centrifuge tube (1), and the flange is configured to be supported on the mouth of the centrifuge tube (1).

3. The osteochondral graft centrifugal device according to claim 2, wherein: The centrifuge tube (1) further comprises a cover body (3) matched with the tube mouth end, the outer diameter of the flange (211) is adapted to the inner diameter of the cover body (3), and the flange (211) is configured to cooperate with the cover body (3) to complete the seal.

4. The osteochondral graft centrifugal device according to claim 2, wherein: The inner cavity of the first bracket (21) is connected to the inner cavity of the second bracket (22), and a supporting platform (4) is provided at the bottom end of the second bracket (22). The supporting platform (4) is provided with a plurality of through-holes (41).

5. The osteochondral graft centrifugal device according to claim 4, characterized in that: The supporting platform (4) is constructed in a cross shape.

6. The osteochondral graft centrifugal device according to claim 1, wherein: The centrifuge tube (1) comprises a tube body (11) and a tapered portion (12) located at the lower end of the tube body (11); the outer wall of the bottom of the second bracket (22) is configured to have a plurality of outwardly extending fixing portions (221) spaced apart along the circumferential direction, the outer edges of the fixing portions (221) being configured to match the inner diameter of the tube body (11), and the second bracket (22) being configured to be supported on the tapered portion (12).

7. The osteochondral graft centrifugal device according to claim 6, wherein: A second notch (222) is formed between two adjacent fixing portions (221), and the second notch (222) is configured to pass through both ends of the fixing portion (221).

8. The osteochondral graft centrifugal device according to claim 6, wherein: A bearing platform (4) is provided at the bottom of the first bracket (21), and a plurality of through-holes (41) are provided on the bearing platform (4).

9. The osteochondral graft centrifugal device according to claim 6, wherein: The bottom of the second bracket (22) is configured to have an open opening (5).

10. The osteochondral graft centrifugal device according to claim 1, wherein A plurality of first openings (212) are evenly spaced along the circumferential direction on the side wall of the first bracket (21), and a plurality of second openings (223) are evenly spaced along the circumferential direction on the side wall of the second bracket (22), wherein the first openings (212) and the second openings (223) are staggered with each other.

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