Growth-promoting rhizobacteria, growth-promoting rhizobacteria microbial inoculum and application of growth-promoting rhizobacteria microbial inoculum

Abstract

The invention discloses a growth-promoting rhizobacteria, a growth-promoting rhizobacteria microbial inoculum and application thereof, and belongs to the technical field of microorganisms, the growth-promoting rhizobacteria is Pseudomonas mohnii, the strain number is YQ142, the classification name is Pseudomonas mohnii, and the growth-promoting rhizobacteria is preserved in China General Microbiological Culture Collection Center on April 7, 2025, and the preservation number is CGMCC No.34110. The invention further discloses a preparation method of the growth-promoting rhizobacteria. The growth-promoting rhizobacteria has strong nitrogen fixation and phosphate solubilizing capabilities, can promote the growth of ryegrass, oat and corn, and improves the saline-alkali stress resistance and drought stress resistance of ryegrass.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a rhizosphere growth-promoting bacterium, a rhizosphere growth-promoting bacterium agent and applications thereof. Background Art

[0002] Soil salinization is a recognized global problem. Based on the current status of soil salinization in my country and the need for green agricultural production, we are developing effective root growth-promoting products, focusing on rhizosphere growth-promoting bacteria. This will contribute to the production of high-quality, high-yield agricultural products and safeguard my country's food security.

[0003] Elymus nutans Griseb., a perennial rhizome grass belonging to the genus Elymus in the Poaceae family, is a sparsely clumping herb. It exhibits wide growth versatility and strong tolerance to cold, drought, and salinity. It thrives on plains, plateau flats, and sunny slopes, gullies, and semi-shaded mountain slopes. Its roots can penetrate up to 88-100 cm into the soil and can utilize deep water, but it cannot tolerate long-term waterlogging. Elymus nutans has a soft texture, is bristly, and odorless, making it easy to prepare into hay. However, its stems become hard upon maturity, reducing its feed value. As a closely related species of wheat, it has important economic value in enriching the wheat gene pool and improving key agronomic traits such as insect resistance, disease resistance, and cold tolerance. Elymus nutans is one of the most widely distributed native grass species on the Qinghai-Tibet Plateau. It is primarily used in artificial grassland establishment, natural grassland vegetation restoration, bioenergy development, saline-alkali land agricultural development, soil remediation and heavy metal pollution control, and windbreak and sand fixation. However, limited research has been conducted on rhizosphere-promoting bacteria that have been screened for their excellent growth-promoting properties in the rhizosphere soil of Elymus nutans. Summary of the Invention

[0004] In response to the shortcomings of the existing technology, the present invention provides a rhizosphere growth-promoting bacteria, a rhizosphere growth-promoting bacteria agent and its application. The rhizosphere growth-promoting bacteria have strong nitrogen fixation and phosphorus solubilization capabilities, can promote the growth of ryegrass, oats and corn, and improve the resistance of ryegrass to salt-alkali stress and drought stress.

[0005] In order to solve the above technical problems, the technical solutions adopted by the present invention are as follows: A rhizosphere growth-promoting bacterium, the rhizosphere growth-promoting bacterium is Pseudomonas mossei, the strain number is YQ142, and the classification name is Pseudomonas mossei Pseudomonas mohnii , deposited in the General Microbiology Center of China Culture Collection of Microorganisms on April 7, 2025, with the deposit number CGMCC No.34110, and the deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0006] A rhizosphere growth-promoting bacteria agent prepared using the rhizosphere growth-promoting bacteria; The bacterial agent is liquid, and the OD 600 is 1-2.

[0007] The rhizosphere growth-promoting bacteria are used to promote the growth of ryegrass, corn and oats and to improve the resistance of ryegrass to salt and alkali stress and drought stress.

[0008] Compared with the prior art, the present invention has the following beneficial effects: The rhizosphere growth-promoting bacteria YQ142 Pseudomonas mossei screened by the present invention has excellent nitrogen fixation, inorganic phosphorus decomposition, organic phosphorus decomposition, IAA production, and iron production capabilities. It can colonize the rhizosphere of plants, and can also promote the growth of ryegrass and its resistance to salt-alkali stress and drought stress. It also has a strong growth-promoting effect on oats and corn. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 The transparent circles are images of Pseudomonas mossei YQ142 in Ashby medium, NBRIP inorganic phosphate medium, and Montgena organic phosphate medium in Example 3; In the figure, the left picture shows the transparent circle of YQ142 Pseudomonas mosei in Ashby medium, the middle picture shows the transparent circle of YQ142 Pseudomonas mosei in NBRIP inorganic phosphate medium, and the right picture shows the transparent circle of YQ142 Pseudomonas mosei in Montkina organic phosphate medium; Figure 2 The growth graphs of three replicates of the plants treated with Pseudomonas mossei YQ142 and the blank control in Example 6 are shown; In the figure, CK1, CK2, and CK3 are three replicates of blank controls, and IN1, IN2, and IN3 are three replicates of plants treated with Pseudomonas mossei in YQ142; Figure 3 This is a comparison chart of the average root length, average plant height, average underground fresh weight, and average aboveground fresh weight of the plants treated with YQ142 Pseudomonas mossei in Example 6 with those of the blank control; In the figure, Control is the blank control, Pseudomonas mohnii is the treatment with YQ142 Pseudomonas mohnii; Figure 4 This is a comparison diagram of the plants treated with Pseudomonas mossei YQ142 in Example 6 after saline-alkali stress and drought stress with the blank control; In the figure, CK is the blank control, S-IN is the inoculation of YQ142 Pseudomonas mossei under saline-alkali conditions, and D-IN is the inoculation of YQ142 Pseudomonas mossei under drought conditions; Figure 5 The growth graph of oats treated with YQ142 Pseudomonas mossei in Example 7 is compared with the blank control; In the figure, CK is the blank control, and IN is the treatment with YQ142 Pseudomonas mossei; Figure 6 The results are a comparison of the yield, number of leaves, and plant height of oats treated with Pseudomonas mossei YQ142 in Example 7 with those of the blank control; In the figure, Control is the blank control, Pseudomonas mohnii is the treatment with YQ142 Pseudomonas mohnii; Figure 7 The results are compared between corn harvested after treatment with YQ142 Pseudomonas mossei and the blank control; In the figure, CK is the blank control, and IN is the YQ142 Pseudomonas morganii treated cell. DETAILED DESCRIPTION

[0010] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific embodiments of the present invention are now described.

[0011] The components of each culture medium in the embodiment are as follows: LB liquid medium: 5.0 g yeast extract powder, 10.0 g tryptone, 10.0 g sodium chloride, 1 L distilled water, adjust the pH to 7.0.

[0012] LB solid medium: yeast extract powder 5.0 g, tryptone 10.0 g, sodium chloride 10.0 g, agar 15.0 g, distilled water 1 L, pH adjusted to 7.0.

[0013] Ashby nitrogen-free medium: mannitol 10.0 g, potassium dihydrogen phosphate 0.2 g, magnesium sulfate heptahydrate 0.2 g, sodium chloride 0.2 g, calcium sulfate heptahydrate 0.1 g, calcium carbonate 5 g, agar 20 g, 0.5% Congo red solution 10 mL, distilled water 1 L, pH adjusted to 7.0.

[0014] NBRIP inorganic phosphate solution medium: sucrose 10.0 g, calcium phosphate 5 g, magnesium chloride 5 g, magnesium sulfate 0.25 g, potassium chloride 0.2 g, ammonium sulfate 0.1 g, agar 20 g, distilled water 1 L, pH adjusted to 7.0.

[0015] Montgena solution organophosphate medium: sucrose 10.0 g, ammonium sulfate 0.5 g, sodium chloride 0.3 g, potassium chloride 0.3 g, magnesium sulfate heptahydrate 0.3 g, ferrous sulfite heptahydrate 0.03 g, manganese sulfate heptahydrate 0.03 g, calcium carbonate 5 g, lecithin 0.025 g, agar 20 g, distilled water 1 L, pH adjusted to 7.0.

[0016] King's medium: 20 g tryptone, 1.5 g potassium hydrogen phosphate, 1.5 g magnesium sulfate, 1 L distilled water, pH adjusted to 7.2.

[0017] MKB medium: 5.0 g casamino acids, 2.5 g potassium hydrogen phosphate, 0.2 g magnesium sulfate heptahydrate, 15.0 mL glycerol, 1 L distilled water, pH adjusted to 7.0.

[0018] Example 1 Isolation and identification of growth-promoting bacteria 1. Sample activation treatment The rhizosphere soil of Elymus nutans stored at -80°C was selected. The rhizosphere soil was collected in Ali, Tibet (31.87N, 80.09E, 5158m above sea level). 1.00g of rhizosphere soil sample was accurately weighed and placed with 100mL of sterile water in a 250mL sterile conical flask, which was then activated in a constant temperature oscillator (28°C, 200rpm) for 30 minutes to obtain a homogeneous soil suspension.

[0019] 2. Gradient Dilution Preparation (1) Establish a sterile gradient system: Take 6 sterile centrifuge tubes and mark them as 10 -1 to 10 -6 For gradient, 900 μL of sterile PBS buffer (pH = 7.2) was added to each tube in advance; (2) Stepwise dilution operation: Take 100 μL of homogenized soil suspension and add it to the first tube (10 -1 ), vortex and shake for 30 seconds to mix, and then transfer 100 μL of the mixture to the secondary tube (10 -2 ), and repeat this operation until the complete dilution gradient is established.

[0020] 3. Microbial Isolation and Culture (1) Environmental pretreatment: After ultraviolet sterilization of the clean bench for 20 minutes, ventilation is run for 15 minutes to form a positive pressure environment, and the alcohol lamp sterile area is maintained throughout the operation; (2) Pre-experimental optimization: The microbial density of each gradient was evaluated by microscopic examination, and the optimal coating concentration was determined based on the pre-culture results (28°C, 12 h); (3) Plate inoculation: Use a sterile pipette to draw 150 μL of the selected gradient dilution solution and evenly spread it on the surface of LB solid medium. Set up 3 biological replicates for each gradient; (4) Culture observation: After coating, seal with Parafilm, place upside down in a constant temperature incubator (28°C) and culture for 12 hours, and observe the colony formation regularly.

[0021] 4. Strain preservation and molecular identification Using the four-zone streaking method, the single colonies in the fourth zone of the purified culture medium were numbered and marked, resulting in a total of 45 single strains, which were then subjected to PCR sequencing to determine which genus the strains belonged to.

[0022] 5. Potted Plant Experiment A potted inoculation test was conducted on the 45 single strains obtained. Specifically, a single colony was picked into a clean LB liquid medium and activated at 28°C for 12 hours. The seed solution was transferred to a new LB liquid medium at 1% (V / V) to further increase the concentration of the bacterial solution. The fermented bacterial solution was then collected by centrifugation at 4°C and 8000 rpm for 5 minutes. The collected precipitated bacteria were resuspended in sterile water and the bacterial solution was adjusted to OD using a spectrophotometer. 600 =1, and then 500uL per plant was used to back-inoculate into potted plants as the inoculation group. Three inoculation pots were set for each strain, and three control pots were set at the same time.

[0023] For the inoculated group, the plants were re-grafted three times at a frequency of once every three days. 14 days after the three re-graftings, the plants were phenotypically observed, and 24 strains of bacteria that promoted plant growth were selected as growth-promoting bacteria.

[0024] 70 μL of the bacterial suspension of the 24 strains screened out from the glycerol collection was added to 630 μL (V:V = 1:10) of LB liquid medium for activation. After activation at 28°C for 12 h, 50 μL was inoculated into 5 mL of LB liquid medium and cultured in a constant temperature shaker (28°C, 200 rpm) until the late logarithmic growth period (OD 600 =3.0) to obtain activated bacterial solution. The purpose of activation is to ensure that the number of viable bacteria reaches the maximum, so that the frozen bacteria can be activated later.

[0025] Then, 1 mL of the activated bacterial suspension of 24 strains was mixed with an equal volume of sterile cryoprotectant (50% glycerol), dispensed into 2 mL cryotubes, labeled, and subjected to gradient cooling (4°C, 2 h; -20°C, 4 h; -80°C, long-term storage) to obtain the preserved glycerol bacteria.

[0026] Example 3 Determination of the ability of strains to fix nitrogen, degrade inorganic phosphorus, and degrade organic phosphorus In this embodiment, when measuring the diameter of the transparent circle, a cross method is used, specifically, the transverse diameter and the vertical diameter in four repetitions are taken to obtain the diameters of 8 colonies, and the average value of the 8 colony diameters is taken.

[0027] Take the preserved glycerol bacteria obtained in Example 1 and melt it on ice to obtain a glycerol bacterial solution. Aspirate the glycerol bacterial solution and add 70 μL of it to 630 μL of fresh LB liquid medium at a ratio of 1:10 (V / V). Transfer it to a 1.5 mL sterile centrifuge tube and place it in a shaker. Incubate at 28°C and 200 rpm to activate the strains until the OD value of each strain reaches 0. 600=1, use a pipette to draw 5μL of bacterial solution and spot it on the upper, lower, left and right points of Ashby culture medium. After the bacterial solution is air-dried, use sealing film to seal it, then put it upside down in a 30℃ constant temperature incubator for 7 days, observe whether there is a nitrogen-fixing transparent circle at the inoculation site, and conduct preliminary strain screening. In order to further confirm the nitrogen-fixing ability, 8 strains with transparent circles, including YQ22, YQ142, YQ9, YQ8, YQ17, YQ16, YQ7, and YQ12, were inoculated in the same way, with 5μL inoculated at each point. Each culture medium was replicated 4 times, and the average diameter of each strain was measured by the cross-cross method. The results are shown in Table 1.

[0028] The phosphate solubilization ability of the above 8 strains was determined. The determination was divided into two parts: determination of the ability to solubilize organic phosphorus and determination of the ability to solubilize inorganic phosphorus. NBRIP inorganic phosphorus solubilization medium and Montgena organic phosphorus solubilization medium were prepared respectively. The preserved glycerol bacteria obtained in Example 1 were taken and thawed on ice. The glycerol bacterial solution was aspirated and added to 630 μL of fresh LB liquid medium at a ratio of 1:10 (V / V). 1.5 mL sterilized centrifuge tubes were used to activate the strains in a shaker at 28°C and 200 r / min until the OD of each strain reached 0. 600 =1, stop the culture, use a pipette to draw 5μL of bacterial solution and spot it on the upper, lower, left and right points of NBRIP solution inorganic phosphorus medium and Montgena solution organic phosphorus medium, wait for the bacterial solution to air dry, seal it with sealing film, then put it upside down in a 30℃ constant temperature incubator for 7 days, observe whether there is a transparent circle at the inoculation site, and then inoculate the strain with a transparent circle in the same way, with 4 replicates for each culture medium, and culture in a 30℃ constant temperature incubator for 7 days after inoculation. Record the diameter of the transparent circle and use the cross method to measure the average diameter of each strain. The results are shown in Tables 2 and 3: Table 1

[0029] Table 2

[0030] Table 3

[0031] From the results in Table 1-3, it can be seen that YQ142 Pseudomonas mossei has excellent nitrogen fixation and inorganic phosphorus decomposition. The transparent circle of YQ142 Pseudomonas mossei in Ashby medium, NBRIP inorganic phosphorus medium and Montkina organic phosphorus medium is shown in the figure. Figure 1 Left, middle and right pictures.

[0032] Example 4 IAA production capacity determination The eight strains obtained from the initial screening in Example 3 were activated (the activation method refers to the method in Example 3, that is, the glycerol bacteria obtained in Example 1 were taken and melted on ice to obtain a glycerol bacterial solution. The glycerol bacterial solution was aspirated and 70 μL was added to 630 μL of fresh LB liquid culture medium at a ratio of 1:10 (V / V). The tubes were transferred to 1.5 mL sterile centrifuge tubes, placed in a shaker, and activated at 28°C and 200 r / min until the OD of each strain reached 0. 600 =1), then inoculated 1% of the culture medium into King's medium supplemented with 0.2 g / L tryptophan. Three replicates were performed for each strain. After culturing in a shaker at 180 rpm and 28°C for 3 days, the cells were ultracentrifuged at 12,000 rpm for 10 minutes. 1 mL of the supernatant was pipetted into a 24-well plate, and an equal volume of Salkowski reagent was added. The plate was wrapped in tin foil and allowed to react at room temperature in the dark for 30 minutes. After the reaction, the absorbance at 530 nm was measured using a microplate reader, and the IAA concentration in the supernatant was calculated using the IAA standard curve. The results are shown in Table 4: Table 4

[0033] It can be seen from the results in Table 4 that YQ142 Pseudomonas mossei has the strongest IAA production ability.

[0034] Example 5 Determination of Siderophore Production Capacity Prepare MKB liquid culture medium. Dispense 50 mL of MKB liquid culture medium into 150 mL Erlenmeyer flasks, sterilize at 121°C for 20 min, and then cool for standby use. Inoculate 50 μL of activated bacterial solution with a pipette in a clean bench (refer to the method in Example 3 for the activation method, i.e., take the preserved glycerol bacteria obtained in Example 1, melt it on ice to obtain a glycerol bacterial solution, draw out the glycerol bacterial solution, add 70 μL of it to 630 μL of fresh LB liquid culture medium at a ratio of 1:10 (V / V), transfer it to a 1.5 mL sterile centrifuge tube, place it in a shaker, and activate it at 28°C and 200 r / min until the OD of each strain reaches 0. 600 = 1), cultured in sterilized, cooled MKB liquid culture medium at 28°C and 180 rpm in a shaking incubator for 48 hours. Centrifuge the shaker culture for 15 minutes at 3500 rpm. Take 3 mL of the supernatant and add it to the CAS test solution and mix thoroughly. After 1 hour, measure the absorbance (A) at a wavelength of 630 nm using a microplate reader. Use double-distilled water as a control to adjust the zero. Take another 3 mL of CAS test solution and mix thoroughly with 3 mL of uninoculated MKB liquid culture supernatant. Measure the absorbance value as the reference value (Ar). Measure the A / Ar ratio. If the bacteria produce iron, it will react with the reaction solution to produce an orange-yellow color. If it does not produce iron, it will not change color and will remain blue. The results are shown in Table 5: Table 5

[0035] It can be seen from the results in Table 5 that YQ142 Pseudomonas mossei has a strong ability to produce iron.

[0036] Example 6 Determination of drought and saline-alkali resistance Vermiculite and nutrient soil were sterilized by moist heat sterilization, 27 7×7 cm flower pots were prepared, sterilized soil was filled in the 7×7 cm flower pots, and 32 sterilized ryegrass seeds of uniform size were evenly dotted. After two weeks of culture, the 8 kinds of preserved glycerol bacteria obtained by the initial screening in Example 3 were taken, melted on ice, and the glycerol bacterial solution was drawn at a ratio of 1:10 (V / V). Specifically, 70 μL of bacterial solution was added to 630 μL of fresh LB liquid medium, and a 1.5 mL sterilized centrifuge tube was used in a shaker to activate at 28°C, 200 r / min for 12 h, transferred to fresh LB liquid medium, added at a ratio of 1:100 (V / V), placed in a shaker, and cultured at 28°C, 200 r / min for 12 h. The culture was centrifuged at 8000 r / min and 4°C for 5 min. After the bacteria were collected, they were resuspended in sterilized water and the OD was adjusted. 600 = 1, and then inoculated into the flower pots, and inoculated into the roots of the ryegrass plants at 100uL / plant. Three flower pots were treated with each bacteria, and the remaining three flower pots were used as blank controls. Inoculation was performed according to the above method, and inoculation was performed once every three days for a total of three times. At the same time, a blank control was set up, and no bacterial solution was inoculated. After 14 days after the three inoculations, the root length, plant height, and fresh weight of all the bacteria were compared. It was found that the effect of the treatment with YQ142 Pseudomonas mori was the best. The growth graph of the plants treated with YQ142 Pseudomonas mori and the three replicates of the blank control is shown in FIG. Figure 2 ; The comparison of the average root length, average plant height, average underground fresh weight and average aboveground fresh weight of plants treated with YQ142 Pseudomonas mossei with those of the blank control is shown in the figure. Figure 3 ; The remaining ryegrass obtained after the above test was subjected to salinity stress and drought stress treatments. The salinity stress treatment was performed by using a 300 mM saline-alkali solution for three days, then changing the gradient to a 400 mM saline-alkali solution for another three days, and then using a 500 mM saline-alkali solution for three days, for a total of 9 days. The drought stress treatment was performed by controlling the soil moisture content to 30% for 10 days. After saline-alkali stress and drought stress, the aboveground biomass, underground biomass, aboveground water content, and underground water content were measured. By comparison, the plants treated with YQ142 Pseudomonas mossii had the best comprehensive index after saline-alkali stress and drought stress. The comparison between the plants treated with YQ142 Pseudomonas mossii and the blank control after saline-alkali stress and drought stress is shown in the figure. Figure 4 .

[0037] Example 7 Determination of the ability of rhizosphere growth-promoting bacteria of Elymus nutans to promote the growth and yield of oats and corn Take the 8 kinds of preserved glycerol bacteria obtained in the initial screening of Example 3, melt them on ice, and draw the glycerol bacterial solution at a ratio of 1:10 (V / V). Specifically, 70 μL of bacterial solution was added to 630 μL of fresh LB liquid medium. Use a 1.5 mL sterilized centrifuge tube in a shaker and activate it at 28 ° C and 200 r / min for 12 h. Transfer it to fresh LB liquid medium and add it at 1:100 (V / V). Place it in a shaker and culture it at 28 ° C and 200 r / min for 12 h. Centrifuge it at 8000 r / min and 4 ° C for 5 min. After collecting the bacteria, resuspend it in sterile water and adjust the OD 600 =2, and then conduct field tests. Specifically, the field planting area is divided into 18 plots, each with an area of ​​3m×3m, numbered 1-18, corn is planted in plots 1-9, and oats are planted in plots 10-18. When planting, the row spacing of corn is 50cm, the plant spacing is 20cm, the row spacing of oats is 20cm, and the sowing width is 7cm (wide strip sowing). The adjusted OD is connected to plots 1-8 and 10-17 respectively. 600 =2, 15 L of each of the eight bacterial cultures was added. Plots 9 and 18 served as blank controls. Following the above-mentioned procedure, the cultures were inoculated twice, every two weeks. After the oats and corn matured, the oat shoots, oat seeds, corn husks, and corn stalks were collected to evaluate the growth-promoting effects of each strain on oats and corn. The results demonstrated that Pseudomonas mossei YQ142 had the most pronounced growth-promoting effect on both oats and corn.

[0038] The growth of oats treated with YQ142 Pseudomonas mossei was compared with the blank control. Figure 5 ; The results of the comparison of the yield, leaf number and plant height of oats treated with YQ142 Pseudomonas mossei and the blank control are shown in Figure 6 ; The results of the comparison between the corn harvested after the treatment of YQ142 Pseudomonas mossei and the blank control are shown in Figure 7 .

[0039] Depend on Figure 5-7 It can be seen that the quality of oats and corn treated with YQ142 Pseudomonas mossei is much better than that of the blank control.

[0040] After screening of Examples 3-7, it was found that YQ142 Pseudomonas mossei had better comprehensive performance.

[0041] Example 8 Morphological Identification The colony characteristics and bacterial morphology of YQ142 Pseudomonas mossei screened in Example 3-7 are as follows: after culturing on LB solid medium for 12 hours, the colonies are light yellow, with smooth edges and a round overall shape. The colony size is 2-3 mm, and the colony surface is moist, smooth and sticky.

[0042] Example 9 Molecular Biology Identification The YQ142 Pseudomonas mossei obtained by screening in Example 3-7 was subjected to molecular biological identification. The identification method and identification results are as follows: 1. PCR reaction system Establish a 30 μL amplification system: • 2×Taq PCR MasterMix 15μL • Template bacterial solution (1:100 dilution) 1μL • Primer 27F / 1492R (10 μM) 1.2 μL each • ddH2O 12.6μL 2. Amplification Procedure

[0043] 3. Product Verification and Sequencing (1) Take 5 μL of PCR product and perform 1.5% agarose gel electrophoresis (120 V, 25 min) to confirm the 1500 bp target band; (2) Use a DNA purification kit to remove primer dimers; (3) The purified product with a concentration of ≥30 ng / μL was sent to Qingke Bio for sequencing.

[0044] The primers used in the product verification and sequencing in step 3 were 27F and 1492R. The sequence of primer 27F is shown in SEQ ID No. 1, and the sequence of primer 1492R is shown in SEQ ID No. 2. The obtained 16S rRNA gene sequence is shown in SEQ ID No. 3.

[0045] YQ142 Pseudomonas mossei was deposited and named as Pseudomonas mossei Pseudomonas mohnii It was deposited in the General Microbiology Center of China Culture Collection Administration on April 7, 2025, with the deposit number CGMCC No.34110, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

Claims

1. A rhizosphere growth-promoting bacterium, characterized in that The rhizosphere growth-promoting bacteria is Pseudomonas mossei, the strain number is YQ142, and the classification name is Pseudomonas mossei Pseudomonas mohnii , deposited in the General Microbiology Center of China Culture Collection of Microorganisms on April 7, 2025, with the deposit number CGMCC No.34110, and the deposit address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

2. The rhizosphere growth-promoting bacteria agent prepared using the rhizosphere growth-promoting bacteria according to claim 1.

3. The rhizosphere growth-promoting bacterial agent according to claim 2, characterized in that The bacterial agent is liquid, and the OD 600 is 1-2.

4. Use of the rhizosphere growth-promoting bacteria according to claim 1 in promoting the growth of ryegrass, corn, and oats and improving the resistance of ryegrass to salt-alkali stress and drought stress.

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