Bacillus P6 as well as fungicide and application thereof
By isolating and applying Bacillus P6 bacterial agent, the problem of weakened cherry roots was solved, the root growth of cherry seedlings was significantly promoted, the root vitality and plant biomass were increased, and the adaptability to adverse soil environment was improved.
Patent Information
- Application Number
- CN202510852811.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-24
- Publication Date
- 2025-09-16
AI Technical Summary
The cherry root system is shallow, with few fibrous roots and low density. It has poor adaptability to adverse soil environments such as drought and waterlogging, is prone to premature aging, and has a short economic fruiting period. Existing microbial growth-promoting bacteria are difficult to be universally applied to all plants.
A strain of Bacillus P6 and its bacterial agent were isolated and provided to promote the growth of cherry roots. By treating cherry seedlings with the bacterial solution, the total root length, root surface area, root volume and average root diameter of the root system were significantly increased, and the root activity was enhanced.
It significantly promotes the root growth and development of cherry seedlings, increases plant biomass, enhances root vitality, and improves the adaptability of cherry to adverse soil environment.
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Figure CN120648614A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of microorganisms, and in particular to a Bacillus P6 strain, a bacterial agent and uses thereof. Background Art
[0002] Cherries are one of the earliest deciduous fruit trees to mature. Compared to other fruit trees, cherries have a weaker root system, characterized by shallow distribution, few fibrous roots, and low root density. They are poorly adapted to adverse soil conditions such as drought and waterlogging. After bearing large quantities of fruit, they are prone to premature aging and a short economic lifespan, among other industry issues.
[0003] Plant growth-promoting rhizobacteria (PGPRs) are a class of beneficial bacteria that promote plant growth. Their species and growth-promoting functions vary widely, but different microorganisms act differently on different plants. Given the specific nature of these microorganisms, it's difficult to generalize their application to all plants. Therefore, it's necessary to isolate a microorganism that promotes root growth and development in cherry trees. Summary of the Invention
[0004] To isolate a microorganism that promotes the root growth and development of cherries, the present invention provides a Bacillus P6 strain, its inoculant, and its uses. The Bacillus P6 strain provided by the present invention has a significant growth-promoting effect on plants, particularly in promoting cucumber seed germination, apple seedling growth, and cherry seedlings.
[0005] The present invention provides a Bacillus ( Bacillus megaterium ) P6, the Bacillus P6 was deposited in the General Microbiology Center of China Culture Collection Administration on April 15, 2025, with the deposit number CGMCC No. 34218, and was classified as Bacillus Bacillus sp.
[0006] The Bacillus P6 provided by the present invention has an obvious growth-promoting effect on plants, has a significant promoting effect on the growth of the radicle and lateral roots after germination of cucumber seeds, has a significant promoting effect on the radicle length and plumule length of apple seedlings, and has a significant growth-promoting effect on cherry seedlings.
[0007] The present invention also provides a bacterial liquid containing the Bacillus P6.
[0008] Furthermore, the OD of the bacterial solution 600 The value is 0.8~1.
[0009] The present invention also provides a plant growth-promoting product, which contains the Bacillus P6 as the only effective ingredient.
[0010] The present invention also provides a use of the Bacillus P6, the bacterial liquid or the plant growth-promoting product in promoting plant growth.
[0011] Furthermore, the plant is cucumber, apple or cherry.
[0012] Furthermore, the promoting of plant growth is promoting the germination of cucumber seeds, promoting the growth of apple seedlings or promoting the growth of cherry plants.
[0013] Furthermore, the promoting of cucumber seed germination is manifested as: promoting the growth of radicle and lateral roots after cucumber seed germination; The promotion of apple seedling growth is manifested in: increasing the radicle length and embryo length of Pingyi sweet tea seeds; Promoting the growth of cherry plants is manifested by: improving the root vitality of cherry seedlings, increasing the total root length, root surface area, root volume and average root diameter of cherry seedlings, and increasing the biomass of cherry seedlings.
[0014] Compared with the prior art, the present invention has the following beneficial effects: The present invention has isolated and obtained a Bacillus ( Bacillus megaterium ) P6, through experimental verification, this strain has a significant growth-promoting effect on plants, which is mainly reflected in its significant promoting effect on cucumber seed germination, significant promoting effect on apple seedling growth, and significant growth-promoting effect on cherry seedlings.
[0015] The growth-promoting effect of Bacillus P6 on cherry seedlings is mainly reflected in promoting the growth and development of the root system of cherry seedlings. After treatment with Bacillus P6, the total root length, root surface area, root volume and average root diameter of the cherry seedlings were significantly increased, and the biomass of the cherry seedlings was significantly increased.
[0016] Information on the deposit of biological materials P6, referred to as Bacillus P6 in this application, was deposited in the General Microbiology Center of China Culture Collection Committee on April 15, 2025, with the deposit number CGMCC No. 34218. The depository address is Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101. The strain is classified and named Bacillus Bacillus sp. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0018] Figure 1 The colony morphology and IAA production qualitative results of Bacillus P6 of the present invention; In the figure, A is the colony morphology of Bacillus P6; B is the qualitative result of IAA production by Bacillus P6.
[0019] Figure 2 The Bacillus P6 of the present invention promotes the germination of cucumber seeds and affects the growth of radicles and lateral roots.
[0020] Figure 3 This is an apparent diagram of the growth-promoting effect of Bacillus P6 of the present invention on Pingyi sweet tea seedlings.
[0021] Figure 4 The present invention shows the effect of the Bacillus P6 on the root activity of cherry Gisela No. 6 seedlings.
[0022] Figure 5 The effect of the Bacillus P6 of the present invention on the maximum photochemical efficiency of cherry Gisela No. 6 seedlings; In the figure, A shows the effect of Bacillus P6 on the maximum photochemical efficiency of the leaves of the cherry Gisela 6 plant; B is the effect of Bacillus P6 on the root respiration rate of cherry Gisela 6 plants; C shows the effect of Bacillus P6 on the respiration rate of various biochemical pathways in the roots of cherry Gisela 6 plants.
[0023] Figure 6 This is an apparent diagram of the growth-promoting effect of the Bacillus P6 of the present invention on the cherry Gisela No. 6 plant.
[0024] Figure 7 The effect of the Bacillus P6 of the present invention on the root topology of the cherry Gisela 6 plant; A is a scanning image of the root system of cherry Gisela 6 plant infected by Bacillus P6; B is the effect of Bacillus P6 on the growth of root hairs of the capillary roots of cherry Gisela 6 plants.
[0025] Figure 8 The effect of the Bacillus P6 treatment of the present invention on the endogenous hormone content in the roots of cherry plants; In the figure, A shows the effect of Bacillus P6 treatment on the indoleacetic acid (IAA) content in the roots of cherry plants; B is the effect of Bacillus P6 treatment on the indoleacetic acid-glutamic acid (IAA-Glu) content in the roots of cherry plants; C is the effect of Bacillus P6 treatment on the indoleacetic acid-aspartic acid (IAA-Asp) content in the roots of cherry plants; D is the effect of Bacillus P6 treatment on the content of gibberellins (GA3) in the roots of cherry plants; E is the effect of Bacillus P6 treatment on the content of trans-zeatin (tZ) in the roots of cherry plants; F is the effect of Bacillus P6 treatment on the abscisic acid (ABA) content in the roots of cherry plants; G is the effect of Bacillus P6 treatment on the content of 1-aminocyclopropanecarboxylic acid (ACC) in the roots of cherry plants; H represents the effect of Bacillus P6 treatment on the jasmonic acid (JA) content in the roots of cherry plants; I shows the effect of Bacillus P6 treatment on the salicylic acid (SA) content in the roots of cherry plants. DETAILED DESCRIPTION
[0026] The specific embodiments of the present invention are described in detail below, but it should be understood that the scope of protection of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial sources unless otherwise specified.
[0027] Example 1: Isolation and identification of a Bacillus strain P6.
[0028] 1. Strain isolation and screening Soil samples were collected on June 28, 2022, from sweet cherry orchards in Yuyao Village, Jiangkui Village, Pulandian District, and Paoya Village, Wafangdian District, Dalian City, Liaoning Province. Well-grown sweet cherry grafted trees with Gisela 6 rootstock were selected for sampling. The topsoil (10 cm thick) was removed, and a 20 cm thick rhizosphere soil was collected using a sterile shovel and placed in a sterile bag. This sample was then stored at -20°C for subsequent isolation and culture of rhizosphere microorganisms.
[0029] Weigh 5g of soil sample and place it in a 250mL Erlenmeyer flask filled with 45mL of sterilized water. Shake it in a shaker at 30℃ and 180r / min for 20min and let it stand for 10min to obtain a soil bacterial suspension. -2 ~10 -8 After gradient dilution, the plate was spread on NB medium, and then the plate was inverted and cultured in a 37°C incubator for 48 hours. Single colonies of different morphologies were picked and purified on the plate for qualitative screening of IAA-producing bacteria.
[0030] Qualitative screening of IAA-producing bacteria: Purified bacteria were inoculated into NB liquid medium containing L-tryptophan (100 mg / L). Three replicates of each strain were cultured at 37°C, 180 rpm, and shaker shaker for 24 hours. A 50 μL droplet of the bacterial culture was then placed on a white ceramic plate. An equal volume of Salkowski colorimetric solution was added. A mixture of 50 μL of uninoculated NB liquid medium and an equal volume of colorimetric solution was used as a control. The white ceramic plate was placed in the dark at room temperature for 30 minutes and then observed. A pink color change was considered positive, indicating IAA secretion. A darker color indicates a stronger secretion. No color change was considered negative, indicating IAA insufficiency.
[0031] 2. 16S rDNA Identification of Strain Preparation of bacterial liquid: The activated strain was inoculated into 50 mL of beef extract peptone liquid medium, cultured at 37 ° C and 180 r / min in a shaking incubator for 24 h, centrifuged at 10,000 r / min for 10 min, discarded the supernatant to collect the bacteria, and then rinsed repeatedly with sterile water and centrifuged at 10,000 r / min for 10 min. Repeat the rinsing step 3 times, and then adjust the bacterial liquid OD with sterile water. 600 The value is 0.8, for backup.
[0032] Using the bacterial suspension of the strain to be tested as a template, universal primers 27F / 1492R were used to amplify 16S rDNA. The complete sequence of 16S rDNA of each strain was then obtained by bidirectional sequencing and splicing. The sequences were then compared in the NCBI database for molecular identification.
[0033] The results are as follows Figure 1 In this example, a strain of IAA-producing bacteria was obtained by qualitative screening of IAA bacteria, named P6, and identified as Bacillus sp. ( Bacillusmegaterium )P6.
[0034] Example 2: Bacillus ( Bacillus megaterium ) Study on the growth-promoting effect of P6 on plants.
[0035] 1. Effects of Bacillus P6 on Cucumber Seed Germination Preparation of Bacillus P6 bacterial suspension: The activated Bacillus P6 strain was inoculated into 50 mL of beef extract peptone liquid culture medium, cultured at 37 ° C and 180 r / min in a shaking incubator for 24 h, centrifuged at 10,000 r / min for 10 min, discarded the supernatant to collect the bacteria, and then rinsed repeatedly with sterile water and centrifuged at 10,000 r / min for 10 min. The rinsing step was repeated three times, and the OD value of the bacterial suspension was adjusted with sterile water. 600 The value is 0.8, and the Bacillus P6 bacterial liquid is obtained for later use.
[0036] Cucumber seed germination test: Select cucumber seeds of roughly uniform size and plumpness. First, disinfect the seeds' surface by soaking them in 2% NaClO for 5 minutes and rinsing them three times with sterile water. Then, soak them in 75% alcohol for 30 seconds and rinse them six times with sterile water. Finally, blot the seeds dry with sterile absorbent paper.
[0037] Surface-sterilized cucumber seeds were soaked in the Bacillus P6 solution prepared above for 6 hours. The seeds were then removed and placed in Petri dishes containing two sterile, moistened filter papers. Fifteen seeds were placed in each dish, with three replicates per treatment. Control seeds were soaked in sterile water for 6 hours and similarly placed in Petri dishes. Finally, the Petri dishes were placed in a 25°C incubator in the dark and observed for germination and growth.
[0038] Bacillus subtilis was purchased from Shanghai Jihe Biotechnology Co., Ltd., with the catalog number HS1759 and the strain accession number ATCC35985, referred to as ATCC35985 strain. Following the above steps, a bacterial suspension of ATCC35985 strain was prepared and a cucumber seed germination test was performed.
[0039] The results are as follows Figure 2 As shown, Bacillus P6 has a significant promoting effect on cucumber seed germination, cucumber radicle and lateral root growth. Although the commercially available strain ATCC35985 also has a certain promoting effect on cucumber seed germination, in comparison, the promoting effect of Bacillus P6 isolated and obtained by the present invention on cucumber seed germination is significantly higher than that of the commercially available strain ATCC35985.
[0040] 2. Effects of Bacillus P6 on the Growth of Pingyi Sweet Tea Seedlings The seeds of Pingyi sweet tea were soaked in water for 24 hours, buried in wet sand, and stratified at 4°C. After 30 days of stratification, the seeds that had initially whitened were selected for preliminary growth promotion screening.
[0041] The selected seeds were first surface disinfected. The specific disinfection method was the same as that of cucumber seeds. The disinfected Pingyi sweet tea seeds were placed in a culture dish with 2 sterile wet filter papers. 15 seeds were placed in each culture dish. Three culture dishes were set up for each treatment. 600 A Bacillus P6 suspension with a pH of 0.8 was added to each seed, with 100 μl of suspension added to each seed. Treatments were repeated every three days for a total of three treatments. An equal amount of sterile water was added to the control. Sterile water was sprayed regularly throughout the treatment period to ensure adequate moisture and humidity for seedling germination and growth. After each treatment, the growth of Pingyi sweet tea seedlings was observed, and relevant indicators such as radicle length, plumule length, plumule diameter, and cotyledon width were measured.
[0042] The same method was used to treat the seeds of Pingyi sweet tea with strain ATCC35985.
[0043] Table 1 Growth-promoting effect of Bacillus P6 on Pingyi sweet tea seeds Note: Data are expressed as mean ± standard deviation (n=4). Different lowercase letters in the same column represent significant differences between different treatments. p <0.05.
[0044] The results are shown in Table 1. Bacillus P6 has a growth-promoting effect on Pingyi sweet tea seeds. However, after the strain ATCC35985 was treated with Pingyi sweet tea seeds, the seeds stopped growing and became moldy, so subsequent experiments were not carried out.
[0045] like Figure 3 As shown, Bacillus P6 has a significant growth-promoting effect on the growth of Pingyi sweet tea seedlings.
[0046] 3. Effects of Bacillus P6 on the Growth of Cherry Gisela 6 Seedlings 1. Experimental methods The 'Gisela 6' cherry seedlings were planted in nutrient pots in advance (the cultivation medium was garden soil: sand = 2:1, and the organic matter content of the mixed medium was 17.42 g·kg -1 , alkaline nitrogen content 127.33 mg•kg -1 , available phosphorus content 173.42 mg•kg -1 , fast-acting potassium content 123.75 mg•kg -1 ). In the experiment, 'Gisela 6' potted seedlings with uniform growth (new shoot length 10 cm) were selected as test materials. Bacillus P6 solution was applied to the roots of Gisela 6 seedlings by root irrigation. The control was treated with sterile water. Five seedlings were treated with Bacillus P6 solution. The OD of the bacterial suspension was 0.01 for each treatment. 600 The value was approximately 0.8, the treatment volume was 50 mL, and treatment was conducted every 5 days for a total of 4 treatments, with a total duration of 20 days. After the treatment, relevant physiological indicators such as root activity, root respiration, root architecture, leaf photosynthesis, chlorophyll content, and plant biomass of the control and treatment groups were measured to preliminarily determine the initial growth-promoting effect of Bacillus P6 on cherry seedlings.
[0047] (1) Determination of plant biomass: On the 20th day after treatment, plant height and stem diameter were measured using a tape measure and an electronic vernier caliper, respectively. After statistical data were collected, the stem, leaves, and roots of the plants were separated and dried at 80°C to constant weight. The dry matter mass, i.e., the biomass of each part, was recorded.
[0048] (2) Determination of plant photosynthetic parameters: The net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), and intercellular carbon dioxide concentration (Ci) of the leaves of 'Gisela 6' seedlings under each treatment were measured using a LI-6800 photosynthetic meter. Three mature leaves (interleaf index = 7-9) were selected for measurement and recorded between 9:00 and 10:00 am on clear days.
[0049] (3) Determination of plant root architecture and topology: The surface area, volume, and total length of the roots were measured using a digital scanner. The roots were washed with distilled water and immersed in a transparent tray filled with distilled water. Scanned images were obtained using a digital scanner (Epson, Long Beach, USA). The scanned images were quantitatively analyzed using WinRhizo PRO 2016 analysis software (Regent Instruments, Quebec, Canada). The root topology characteristics were classified as multi-branched or herringbone-shaped in terms of branching structure. The external link length (Pe) refers to the sum of all path links from each external link to the base link.
[0050] (4) Determination of root activity: Weigh 0.5 g of fresh root sample, add 5 mL of triphenyltetrazolium chloride (0.4% by mass to volume) and 5 mL of phosphate buffer (pH 7.0), and incubate at 37°C for 4 h. Then, add 2 mL of 1 mol / L H2SO4 solution to terminate the reaction. Remove the root, wipe dry, and return it to the original test tube. Add 10 mL of 95% ethanol solution and extract overnight. When the solution turns red, perform colorimetric analysis at 485 nm.
[0051] (5) Determination of root respiration: The Hansatech (UK) OxyTrace+ liquid oxygen electrode automatic measurement system was used to measure the respiration rates of the glycolysis pathway, tricarboxylic acid cycle pathway, and pentose phosphate pathway. Sodium fluoride (0.5 mol / L), malonic acid (0.5 mol / L), and sodium phosphate (0.5 mol / L) were added to the liquid oxygen electrode reaction chamber in sequence to measure the respiration rates of the glycolysis pathway, tricarboxylic acid cycle pathway, and pentose phosphate pathway. 2 ml of phosphate buffer was added to the oxygen electrode reaction chamber, and approximately 0.05 g of young roots of 'Gisela No. 6' cherry were weighed and added to the reaction chamber. After the slope of the software OxyTrace+ (Version: v1.0.48) stabilized, the slope was intercepted and the total root respiration rate R was calculated based on the decreasing slope of the oxygen concentration. total = -2*0.001*slope / root fresh weight. Add 50 μl of sodium fluoride (0.5 mol / L) and wait for the slope to stabilize. Then, intercept the slope and substitute it into the total respiration rate formula. Glycolytic respiration rate: R EMP =R total -R NaF , R TCA =Rtotal - R EMP - R 丙二酸 , R TCA =R total - R EMP – R TCA -R Na3PO4 .
[0052] 2. Experimental results (1) Effects of Bacillus P6 on root activity of Gisela 6 seedlings The results are as follows Figure 4 As shown, Bacillus P6 significantly improved the root activity of Gisela 6 seedlings.
[0053] (2) Effects of Bacillus P6 on photosynthetic parameters and maximum photochemical efficiency of Gisela 6 seedlings Table 2 Effects of Bacillus P6 on photosynthetic characteristics of cherry seedlings Note: Data are expressed as mean ± standard deviation (n=4). Different lowercase letters in the same column represent significant differences between different treatments. p <0.05.
[0054] As shown in Table 2, Bacillus P6 significantly increased the Pn of cherry seedlings compared with CK, with a significant increase of 48.43% compared with CK. In terms of Tr, Gs, and Ci, Bacillus P6 significantly inhibited the photosynthetic characteristics of cherry seedlings to varying degrees compared with CK.
[0055] like Figure 5 As shown in the results, after root irrigation, the P6 treatment significantly inhibited the maximum photochemical efficiency of cherry plant leaves, significantly reducing it by 3.17% compared to the CK. The P6 treatment increased the root respiration rate by 70.09% compared to the CK. The P6 treatment had the most significant effect on the EMP respiration rate, increasing it by 283.67% compared to the CK. The P6 treatment also increased the PPP respiration rate by 91.43%.
[0056] (3) Effects of Bacillus P6 on the biomass of Gisela 6 seedlings Table 3 Effects of Bacillus P6 on the biomass of Gisela 6 seedlings Note: Data are expressed as mean ± standard deviation (n=5). Different lowercase letters in the same column represent significant differences between different treatments. p <0.05.
[0057] The results are shown in Table 3 and Figure 6As shown in the figure, Bacillus P6 increased the plant height of Gisela 6 seedlings by 76.70% compared with CK, and the difference was significant. Bacillus P6 treatment significantly increased the aboveground biomass compared with CK.
[0058] (4) Effects of Bacillus P6 on the root architecture, topology, and root hair growth of Gisela 6 seedlings Table 4 Effects of Bacillus P6 treatment on root morphology of cherry plants Note: Data are expressed as mean ± standard deviation (n=3). Different lowercase letters in the same column represent significant differences between different treatments. p <0.05.
[0059] As shown in Table 4, Bacillus P6 treatment affected the root architecture of cherry seedlings. After treatment with Bacillus P6, the total root length of cherry seedlings increased significantly by 23.77% compared with the control (CK), the root surface area increased significantly by 37.73%, the root volume increased significantly by 56.34%, and the average root diameter increased significantly by 11.54%. Therefore, Bacillus P6 treatment significantly affected the root morphology of cherry plants, promoting root growth and development.
[0060] Table 5 Effects of Bacillus P6 treatment on root topology of cherry plants Note: Data are expressed as mean ± standard deviation (n=3). Different lowercase letters in the same column represent significant differences between different treatments. p <0.05.
[0061] As shown in Table 5 and Figure 7 As shown in the figure, Bacillus P6 treatment affected the root topology of cherry seedlings. Bacillus P6 treatment of cherry seedlings significantly increased the number of root branches and links.
[0062] (5) Effects of Bacillus P6 treatment on endogenous hormone content in the roots of cherry plants The results are as follows Figure 8 As shown in the results, Bacillus P6 treatment significantly affected the contents of bound auxins IAA-Glu and IAA-Asp. The IAA-Glu content in the roots of cherry plants treated with Bacillus P6 increased by 36.87% and 90.71% compared to CK, respectively, with both differences being significant.
[0063] Treatment with Bacillus sp. P6 significantly increased endogenous ACC content in the roots by 24.99% compared to the CK, and endogenous SA content by 32.42%. Treatment with P6 significantly decreased endogenous ABA content in the roots by 58.35% compared to the CK, and also significantly decreased endogenous JA content by 27.74%. Therefore, changes in the levels of various endogenous hormones in cherry roots indicate that treatment with P6 significantly altered endogenous hormone levels in the rhizosphere, promoting root growth and development.
[0064] Although preferred embodiments of the present invention have been described, additional changes and modifications to these embodiments may occur to those skilled in the art once the basic inventive concepts become known.
[0065] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims and their equivalents, the present invention is intended to include such modifications and variations.
Claims
1. A strain of Bacillus ( Bacillus megaterium ) P6, characterized in that, The Bacillus P6 was deposited in the General Microbiology Center of China Culture Collection Administration on April 15, 2025, with the deposit number CGMCC No. 34218, and was classified as Bacillus Bacillus sp.
2. A bacterial liquid, characterized in that: The bacterial solution contains the Bacillus P6 according to claim 1.
3. The bacterial solution according to claim 2, characterized in that The OD of the bacterial solution 600 The value is 0.8~1.
4. A plant growth promotion product, characterized in that: The plant growth promotion product contains the Bacillus P6 described in claim 1 as the only active ingredient.
5. Use of the Bacillus P6 according to claim 1, the bacterial solution according to any one of claims 2 to 3, or the plant growth-promoting product according to claim 4 in promoting plant growth.
6. The use according to claim 5, characterized in that The plant is cucumber, apple or cherry.
7. The use according to claim 6, characterized in that The promoting of plant growth is promoting the germination of cucumber seeds, promoting the growth of apple seedlings or promoting the growth of cherry plants.
8. The use according to claim 7, characterized in that The promoting of cucumber seed germination is manifested in: promoting the growth of radicle and lateral roots after cucumber seed germination; The promotion of apple seedling growth is manifested in: increasing the radicle length and embryo length of Pingyi sweet tea seeds; Promoting the growth of cherry plants is manifested by: improving the root vitality of cherry seedlings, increasing the total root length, root surface area, root volume and average root diameter of cherry seedlings, and increasing the biomass of cherry seedlings.