Application of LNC1 gene in regulating and controlling plant type of medicago truncatula or soybean plant

By using CRISPR/Cas9 technology to target and knock out the LNC1 gene, the petiole angle of Medicago truncatula and soybean was changed, solving the problem of insufficient petiole angle regulation in existing technologies, and achieving plant shape optimization and improved efficiency of high-density planting.

CN120648695APending Publication Date: 2025-09-16XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202510630987.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-16
Publication Date
2025-09-16

AI Technical Summary

Technical Problem

In the existing technology, plant type improvement of legume crops such as Medicago truncatula and soybean mainly focuses on stem height and branch angle, and there is a lack of research on the genetic regulation of petiole angle, which limits the efficiency of plant type optimization and high-density planting.

Method used

Through CRISPR/Cas9 gene editing technology, the LNC1-alfalfa gene in Medicago truncatula and the LNC1a and LNC1b genes in soybeans were targeted and knocked out to achieve functional loss, change the petiole angle of the plant, and thus regulate the plant shape.

Benefits of technology

The petiole angle of Medicago truncatula and soybean plants was significantly increased, achieving effective regulation of plant shape and providing genetic resources for high-density planting and mechanized harvesting.

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Abstract

The invention discloses application of an LNC1 gene in regulating and controlling plant types of medicago truncatula or soybean plants, and belongs to the technical field of plant type gene regulation and control. According to related tests, an LNC1-alfalfa gene in medicago truncatula and an LNC1a gene and an LNC1b gene in soybean are homologous LNC1 genes, and the petiole included angle of the medicago truncatula plant and the petiole included angle of the soybean plant can be remarkably increased through function deletion of the LNC1-alfalfa gene and simultaneous function deletion of the LNC1a gene and the LNC1b gene respectively; namely, the change of the petiole included angle of the leguminous plant medicago truncatula and the soybean plant can be realized through the function deletion of the homologous LNC1 gene, so that the plant type regulation and control are realized, and the gene has a wide application prospect in the aspects of leguminous plant type regulation and control and the like.
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Claims

1. A LNC1 The application of the gene in regulating the plant type of Medicago truncatula or soybean is characterized in that: LNC1 Alfalfa LNC1- alfalfa genes and their homologs in soybean LNC1a Genes and LNC1b Gene; LNC1- The nucleotide sequence of the alfalfa gene is shown in SEQ ID No. 1, LNC1 The amino acid sequence of the gene-encoded protein is shown in SEQ ID No.

2. LNC1a The nucleotide sequence of the gene is shown in SEQ ID No. 3, LNC1a The amino acid sequence of the gene-encoded protein is shown in SEQ ID No.

4. LNC1b The nucleotide sequence of the gene is shown in SEQ ID No.5, LNC1b The amino acid sequence of the gene-encoded protein is shown in SEQ ID No.6; LNC1- The loss of alfalfa gene function can increase the petiole angle of Medicago truncatula plants, through LNC1a Gene 、LNC1b The simultaneous loss of gene function can increase the petiole angle of soybean plants.

2. The use according to claim 1, characterized in that LNC1- Retrotransposons Tnt1 Insert into LNC1- On the exons of alfalfa genes.

3. The use according to claim 1, characterized in that LNC1a Gene 、LNC1b The simultaneous loss of gene function was achieved by constructing a gene editing vector using CRISPR-Cas9 technology to target and knock out soybean LNC1a Genes and LNC1b Gene.

4. The use according to claim 3, characterized in that Targeted knockout in soybean LNC1a Genes and LNC1b Genetic methods include: (1) Construct LNC1a Genes and LNC1b CRISPR / Cas9 gene editing vectors for genes; (2) The constructed CRISPR / Cas9 gene editing vector was transformed into soybean cells through Agrobacterium infection.