Method for rapidly identifying ovulation of procambarus clarkii

Abstract

The invention discloses a rapid method for identifying ovulation of procambarus clarkii. According to the method, whether the procambarus clarkii has oviposition or ovary development is judged according to morphological characteristics of the procambarus clarkii. The method comprises the following steps that female shrimps with the belly and the tail cleaned are taken, the belly of each female shrimp is observed firstly, the shrimp line of the belly of each ovulated shrimp is not obvious or even does not exist, and then abdominal muscles are loose and have no elasticity. Meanwhile, white spots exist at the tails of the ovulated female shrimps, and whether the procambarus clarkia ovulates or not can be rapidly judged on the premise that the procambarus clarkia is not dissected according to the characteristics. In previous breeding work, ovulated female shrimps and male shrimps are frequently paired due to absence of a method for judging whether the female shrimps are ovulated or not, so that the pairing efficiency is low. According to the method, whether the procambarus clarkia ovulates or not can be rapidly distinguished, the problem that in previous breeding work, oviposition of female shrimps is not uniform is solved, the oviposition shrimps and the non-oviposition shrimps are simply and directly distinguished, researchers can select the non-oviposition female shrimps for pairing, the purpose of large-scale unification of seedlings is achieved, and the breeding efficiency of the procambarus clarkia is improved. And a foundation is further laid for breeding of the procambarus clarkii.

Description

Technical Field

[0001] The invention relates to the technical field of modern biological breeding, in particular to a method for rapidly identifying the ovulation of Procambarus clarkii. Background Art

[0002] Procambarus clarkii, also known as crayfish, belongs to the family Cheliceridae of the order Decapoda in the class Crustacea. The crayfish industry has experienced rapid growth in recent years, with Changsha alone consuming one million tons of crayfish daily during peak crayfish season. However, the quality of the crayfish has also declined significantly in recent years, primarily manifested in smaller size and lower meat yield. This is attributed to a shortage of high-quality crayfish seed, which has severely hampered the industry's development. To address this issue, some researchers have attempted to breed Crayfish, primarily through hybridization. However, existing Crayfish aquaculture systems lack a simple and rapid method for ensuring female ovulation. Ovulated females will not mate with sexually mature males, resulting in significant time and expense for researchers and severely hindering progress in Crayfish research. Being able to identify and pair unovulated females could significantly reduce this waste of time and money and improve breeding efficiency.

[0003] In most fish breeding, farmers can generally determine ovulation status based on conventional body morphological characteristics. For example, most female fish will have a swollen abdomen and a slightly reddish genital pore when they haven't ovulated. Gently squeezing their abdomen will cause some eggs to flow out of the genital pore. However, the hard and opaque chitinous shell of Procambarus clarkii makes it impossible to extract their eggs by squeezing, making it difficult to determine female ovulation status during production research. Previously, ovulation status could only be determined empirically, but due to the lack of intuitive, quantitative, and scientific features, the accuracy of the determination was low. Therefore, identifying intuitive and scientific external morphological characteristics that can quickly determine ovulation status is of great significance for crayfish breeding and aquaculture. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem of asynchronous egg release during the artificial pairing of Procambarus clarkii, provide a method for quickly distinguishing the ovulation status of Procambarus clarkii, provide a direct basis for quickly screening non-ovulated Procambarus clarkii, reduce the situation of blind pairing due to different ovulation status, facilitate the pairing and breeding of Procambarus clarkii to reduce time and financial costs, and further promote the development of the Procambarus clarkii industry.

[0005] To achieve the above objectives, the present invention provides the following solutions:

[0006] A method for rapidly identifying ovulation of Procambarus clarkii, comprising:

[0007] To determine the sex of Procambarus clarkii, only take the female shrimp;

[0008] Clean the abdomen of the female shrimp to ensure it is clean and free of foreign matter;

[0009] Observe the shrimp line on the abdomen. If there is an obvious shrimp line, give up the judgment directly. If there is no obvious shrimp line, proceed to the next step.

[0010] Observe the black spots on the abdomen. If there is no obvious shrimp line, give up the judgment directly. If there is an obvious shrimp line, proceed to the next step.

[0011] Gently squeeze the abdomen of the female shrimp to judge the muscle condition. If the abdominal muscles are loose and lack elasticity, it is a female shrimp that has ovulated. If the abdominal muscles of the female shrimp are firm and elastic, it is a female shrimp that has not ovulated.

[0012] In one embodiment of the present invention, the identified Procambarus clarkii is in good growth condition and weighs about 15-40 g.

[0013] In one embodiment of the present invention, the living water temperature is controlled at 15-25°C, the dissolved oxygen content is maintained at above 2.0 mg / L, the pH is within the range of 7.0-8.5, the ammonia nitrogen content is below 0.5 mg / L, the nitrite content is below 0.1 mg / L, the hydrogen sulfide content is below 0.03 mg / L, and the total hardness range is within 50-100 mg / L.

[0014] In one embodiment of the present invention, the shrimp in the sewage area needs to be cleaned to improve accuracy.

[0015] In one embodiment of the present invention, after the crayfish is identified, it should be promptly transferred to its original water environment to avoid unnecessary damage to the crayfish. The positive effects of the present invention are:

[0016] The identification method of the present invention can quickly determine the spawning situation of Procambarus clarkii without causing any harm to the crayfish, effectively overcoming the defect that conventional methods cannot accurately determine the ovulation situation of female shrimps, and providing technical support for their genetic breeding work. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work. Description of the drawings:

[0019] Figure 1 A female with more silt attached is shown.

[0020] Figure 2 Display image of a female shrimp with distinct shrimp lines.

[0021] Figure 3 A picture of a female shrimp without obvious shrimp lines.

[0022] Figure 4 A female shrimp displays obvious black spots.

[0023] Figure 5 Diagram of the 5 parts of the female shrimp abdomen.

[0024] Figure 6 Judgment flow chart.

Claims

1. A method for rapidly identifying whether a crayfish has ovulated, characterized in that: The following steps are involved: By judging the abdominal morphological characteristics of female shrimp, the ovulation status of Procambarus clarkii can be determined; The morphological characteristics are mainly based on observing the shrimp line and black spots on the abdomen, and judging in combination with the condition of the abdominal muscles.

2. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: The identified crayfish were in good growth condition and weighed about 15-40g.

3. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: The water temperature for its survival is controlled at 15-25℃, the dissolved oxygen content is maintained at above 2.0mg / L, the pH is within the range of 7.0-8.5, the ammonia nitrogen content is below 0.5mg / L, the nitrite content is below 0.1mg / L, the hydrogen sulfide content is below 0.03mg / L, and the total hardness range is within 50-100mg / L.

4. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: The object of observation must be female shrimp, and there must be no mud or other obstructions on the abdomen. If there is mud, you can gently wipe it with a fine-bristled brush until the mud disappears.

5. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: If the shrimp line on the abdomen is obvious, ovulation has not occurred. If the shrimp line is not obvious, proceed to the next step to make the judgment.

6. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: For shrimps with clear shrimp lines, observe the black spots on their abdomens. If there are no black spots, they have not ovulated. If there are black spots, proceed to the next step.

7. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: The abdomen of female shrimp is divided into five areas according to body segments, at least one of which must have obvious black spots.

8. The method for rapidly identifying whether Procambarus clarkii has ovulated according to claim 1, characterized in that: Gently squeeze the abdomen of the female shrimp. If its abdominal muscles are loose and lack elasticity, it is a female shrimp that has ovulated. If the abdominal muscles of the female shrimp are firm and elastic, it is a female shrimp that has not ovulated.

9. A female shrimp identified by the method according to any one of claims 1 to 7, characterized in that: The morphological characteristics of Procambarus clarkii were used to quickly obtain ovulated female Procambarus clarkii.

10. The innovation of this method lies in that it is the first time that the ovulation status of Procambarus clarkii is determined by its physical characteristics. It is fast and accurate, and can quickly and accurately distinguish whether the female shrimp has ovulated or not. It is simple, immediate and easy to operate.

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