A method for improving colistin e wastewater treatment capacity

Abstract

The application discloses a method for improving colistin E wastewater treatment capacity, and belongs to the technical field of wastewater treatment. Specifically, the method comprises the following steps: A, taking activated sludge and placing the activated sludge in a reactor; B, taking colistin E production wastewater with additional cyclic diguanidate, and making the colistin E production wastewater flow through the reactor at a certain organic load. The colistin E production wastewater has a toxic effect on the microorganisms in the activated sludge in many aspects, so that the growth of the microorganisms is inhibited. The cyclic diguanidate is a key regulating factor for synthesizing extracellular matrix, and it can activate a series of genes participating in the synthesis of components such as extracellular polysaccharide and protein, so that the microorganisms can produce a large amount of extracellular matrix. The extracellular matrix is a main component of a biofilm, and can provide protection and a stable living environment for the microorganisms. The experiment proves that the colistin E production wastewater with additional cyclic diguanidate can effectively improve the wastewater treatment capacity.

Description

Technical Field

[0001] This invention relates to the field of wastewater treatment technology, and in particular to a method for improving the wastewater treatment capacity of colistin E. Background Technology

[0002] Colistin E is a polypeptide antibiotic primarily used to prevent and treat infections caused by susceptible bacteria and to promote the growth of livestock and poultry. Colistin E binds to free phosphate groups of cell membrane lipoproteins, reducing cell membrane surface tension and increasing permeability, leading to cytoplasmic leakage and cell death. Colistin E has a strong inhibitory effect on Gram-negative bacteria (especially Escherichia coli, Salmonella, Pseudomonas aeruginosa, Proteus, and Haemophilus), but has no effect on Gram-positive bacteria (except Staphylococcus aureus and hemolytic streptococci) and fungi.

[0003] The production of colistin E generates a large amount of wastewater, requiring significant human, material, and financial resources for treatment. When treatment capacity is limited, production capacity must be sacrificed to meet discharge standards; otherwise, it will have adverse environmental impacts. Therefore, improving wastewater treatment capacity is crucial.

[0004] Colistin E production wastewater has multiple toxic effects on microorganisms in activated sludge. For example, colistin can damage bacterial cell membrane structures, causing intracellular substances to leak out, affecting cellular metabolic activities, and thus inhibiting microbial growth and reproduction. The wastewater may also contain high concentrations of nutrients such as nitrogen and phosphorus, as well as some recalcitrant organic matter, such as polysaccharides and proteins, leading to nutrient imbalances in microorganisms, affecting their normal metabolic pathways, and inhibiting microbial growth. Cycloguanosine monophosphate (CGM) is a key regulatory factor in the synthesis of the extracellular matrix. It activates a series of genes involved in the synthesis of extracellular polysaccharides, proteins, and other components, enabling microorganisms to produce large amounts of extracellular matrix. This extracellular matrix is ​​a major component of biofilms, providing protection and a stable living environment for microorganisms. Therefore, introducing CGM in wastewater treatment is beneficial for maintaining biological activity and thus improving wastewater treatment capacity. Summary of the Invention

[0005] Purpose of the invention: The purpose of this invention is to provide a method for improving the treatment capacity of colistin E wastewater, thereby addressing the problem of improving wastewater treatment capacity.

[0006] Technical Solution: To solve the above-mentioned technical problems, according to one aspect of the present invention, more specifically, a method for improving the treatment capacity of colistin E wastewater, specifically including the following steps:

[0007] A. Place the activated sludge into the reactor;

[0008] B. Take the wastewater from the production of colistin E with added cyclic guanosine monophosphate at a concentration of 0.1-20 kg COD / (m³). 3The organic load of *d) flows through the reactor.

[0009] Furthermore, the temperature of the reactor in step A is controlled at 35±5℃.

[0010] Furthermore, the temperature of the reactor in step A is controlled at 35±2℃.

[0011] Furthermore, in step B, the organic loading range of the colistin E production wastewater is 1-10 kg COD / (m³). 3 *d).

[0012] Furthermore, in step B, the organic loading range of the colistin E production wastewater is 3-7 kg COD / (m³). 3 *d).

[0013] Furthermore, in step B, the organic load range of the colistin E production wastewater is 5 kg COD / (m³). 3 *d).

[0014] Furthermore, in step B, the concentration of cyclic diguanosine monophosphate added is 0.1-10 μM.

[0015] Furthermore, in step B, the concentration of cyclic diguanosine monophosphate added is 0.5-2 μM.

[0016] Furthermore, in step B, the colistin E production wastewater flows from the bottom to the top, and the fermentation method is anaerobic fermentation.

[0017] Beneficial effects: The key technology of this invention is to place activated sludge in a reactor; and to pass colistin E production wastewater with added cyclic diguanylic acid through the reactor at a certain organic load. The addition of cyclic diguanylic acid to the colistin E production wastewater effectively improves wastewater treatment capacity. Experiments of this invention have shown that the COD removal rate is 8.5% higher than that of conventional technologies. Detailed Implementation

[0018] To make the technical solution of the present invention clearer, the present invention will be further described in detail below with reference to specific embodiments.

[0019] Example 1

[0020] Activated sludge was placed in the reactor, and the temperature was controlled at 35±2℃. Colistin E production wastewater was treated at a concentration of 5 kg COD / (m³). 3 *d) Organic load flows through the reactor. The colistin E production wastewater flows from the bottom inlet to the top outlet, and the fermentation method is anaerobic fermentation.

[0021] The test results on day 15 of the reaction are shown in Table 1:

[0022] Table 1: Test Results of Example 1

[0023] Example 1 3501 812 76.8％

[0024] Example 2

[0025] The difference from Example 1 is that cycloguanosine monophosphate was added to the colistin E production wastewater at a concentration of 0.5 μM.

[0026] The test results on day 15 of the reaction are shown in Table 2:

[0027] Table 2: Test Results of Example 2

[0028] Example 2 3510 721 79.5％

[0029] Example 3

[0030] The difference from Example 2 is that the concentration of cyclic diguanosine monophosphate added is 1 μM.

[0031] The test results on day 15 of the reaction are shown in Table 3:

[0032] Table 3: Test Results of Example 3

[0033] Example 3 3505 647 81.5％

[0034] Example 4

[0035] The difference from Example 2 is that the concentration of the additional cyclic diguanosine monophosphate is 1.5 μM.

[0036] The test results on day 15 of the reaction are shown in Table 4:

[0037] Table 4: Test Results of Example 4

[0038] Example 4 3515 588 83.3％

[0039] Example 5

[0040] The difference from Example 2 is that the concentration of cyclic diguanosine monophosphate added is 2 μM.

[0041] The test results on day 15 of the reaction are shown in Table 5:

[0042] Table 5: Test Results of Example 5

[0043] Example 5 3506 616 82.4％

[0044] As can be seen from the above examples, the addition of cyclic diguanylic acid to the colistin E production wastewater improves the COD removal rate. Among them, Example 4 shows the greatest improvement in removal rate, increasing by 8.5% compared to Example 1. These examples demonstrate that the present invention can improve the treatment capacity of colistin E wastewater.

[0045] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A method for improving the treatment capacity of colistin E wastewater, characterized in that, Specifically, the following steps are included: A. Place the activated sludge into the reactor; B. Take the wastewater from the production of colistin E with added cyclic diguanylic acid at a concentration of 0.1-20 kg COD / (m³). 3 *d) Organic load flows through the reactor; In step B, the concentration of the additional cyclic diguanosine monophosphate is 0.1-10 μM; in step B, the colistin E production wastewater flows from the bottom to the top, and the fermentation method is anaerobic fermentation.

2. The method for improving the treatment capacity of colistin E wastewater according to claim 1, characterized in that: In step A, the reactor temperature is controlled at 35±5℃.

3. The method for improving the treatment capacity of colistin E wastewater according to claim 2, characterized in that: In step A, the temperature of the reactor is controlled at 35±2℃.

4. The method for improving the treatment capacity of colistin E wastewater according to claim 1, characterized in that: In step B, the organic load range of the colistin E production wastewater is 1-10 kg COD / (m³). 3 *d).

5. The method for improving the treatment capacity of colistin E wastewater according to claim 4, characterized in that: In step B, the organic load range of the colistin E production wastewater is 3-7 kg COD / (m³). 3 *d).

6. The method for improving the treatment capacity of colistin E wastewater according to claim 5, characterized in that: In step B, the organic load range of the colistin E production wastewater is 5 kg COD / (m³). 3 *d).

7. The method for improving the treatment capacity of colistin E wastewater according to claim 1, characterized in that: In step B, the concentration of the additional cyclic diguanosine monophosphate added is 0.5-2 μM.