Diatom rice protein feed as well as preparation method and application thereof

Abstract

The invention discloses diatom rice protein feed as well as a preparation method and application thereof, and belongs to the technical field of animal breeding. The diatom rice protein feed comprises a component A, a component B and a component C, the component A comprises glutinous rice, rice, rice bran, sweet distiller's yeast, arrack distiller's yeast, beer yeast, dry yeast powder, fresh malt, diatom powder and phyllostachys pubescens powder; the component B comprises Xuefeng cordyceps fungus, silkworm fungus, astragalus membranaceus, liquorice, codonopsis pilosula, caulis spatholobi, folium isatidis powder and mulberry leaf powder, and the component C comprises rice, fresh papaya juice, walnut powder, edible soda ash, salt, loach mucus or catfish mucus, brown sugar and banana jam. The diatom and rice protein feed disclosed by the invention is rich in nutrition, high in grade, simple in process, low in raw material cost, production cost and energy consumption cost, very wide in application way and good in market prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of animal breeding, and more particularly to a diatom rice protein feed and a preparation method and application thereof. Background Art

[0002] China is a major producer of pigs and meat. It also leads the world in cattle, sheep, and poultry farming. Therefore, the current and future development of the feed industry is undoubtedly of great strategic significance.

[0003] Therefore, creatively developing protein feed resources and their production is undoubtedly the top priority in the high-quality development of my country's breeding industry. Summary of the Invention

[0004] In view of this, the object of the present invention is to provide a diatom rice protein feed and a preparation method and application thereof, so as to overcome the deficiencies in the prior art.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A diatom rice protein feed comprises component A, component B and component C;

[0007] Component A comprises the following raw materials in parts by weight: 55-70 parts of glutinous rice, 15-18 parts of rice, 5-7 parts of rice bran, 2-3 parts of sweet wine koji, 1-2 parts of shochu koji, 0.5-1 part of brewer's yeast, 0.5-0.7 part of dry yeast powder, 3-5 parts of fresh malt, 2-2.6 parts of diatom powder, and 3-4 parts of bamboo powder;

[0008] Component B comprises the following raw materials in parts by weight: 0.1-0.3 parts of Xuefeng Cordyceps, 1-1.5 parts of Bombyx mori, 2-2.5 parts of Astragalus, 1-1.8 parts of Licorice, 2-2.3 parts of Codonopsis pilosula, 2.5-3 parts of Millettia reticulata, 3.2-4 parts of Isatis indigotica powder, and 4.1-5.2 parts of mulberry leaf powder;

[0009] Component C comprises the following raw materials in parts by weight: 60-70 parts of cooked rice, 2-3 parts of fresh papaya juice, 1-2 parts of walnut powder, 1.5-5.2 parts of edible baking soda, 0.5-1 part of salt, 1.5-2.0 parts of loach mucus or catfish mucus, 3-4 parts of brown sugar and 4-5 parts of banana paste.

[0010] A method for preparing a diatom rice protein feed specifically comprises the following steps:

[0011] (1) weighing each raw material by parts by weight of above-mentioned diatom rice protein feed;

[0012] (2) Mix glutinous rice, rice and rice bran evenly, steam them, and cool them to obtain material A, which is then set aside;

[0013] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to obtain material B;

[0014] (4) After fully mixing material A and material B, sealing and fermenting to obtain a diatom rice polysaccharide product;

[0015] (5) Astragalus, Licorice, Codonopsis pilosula and Millettia repens are mixed and crushed and sieved, and then added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented to obtain the diatom rice polysaccharide and protein product;

[0016] (6) mixing Folium Isatidis powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting to obtain the diatom rice polysaccharide and protein product;

[0017] (7) Mix rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus or catfish mucus, brown sugar, and banana paste, add to the diatom rice multibacterial enzyme and protein product, stir evenly, seal and ferment, spread to attract flies to eat, mate, and lay eggs, cultivate maggots, scald the maggots to death, add salt, mix thoroughly, and then naturally cool or dry to obtain diatom rice protein feed.

[0018] Furthermore, in the above step (2), the temperature is cooled to 15-18°C.

[0019] Furthermore, in the above step (3), the powder is crushed to 100 mesh.

[0020] Furthermore, in the above step (4), the temperature of the sealed fermentation is 18-35°C and the time is 12-72 hours.

[0021] Furthermore, in the above step (5), the temperature of the sealed fermentation is 22-34° C. and the time is 3-7 h.

[0022] Furthermore, in the above step (6), the temperature of the semi-sealed fermentation is 18-34° C. and the time is 24-72 hours.

[0023] Furthermore, in the above step (7), the temperature of the sealed fermentation is 19-35°C, and the time is 24-32 hours; the time for feeding, mating, and laying eggs is 3-4 hours; and the humidity of the maggots is 70%-80%, the temperature is 25-32°C, and the time is 80-100 hours.

[0024] Furthermore, in the above step (7), the mass of added salt is 0.8%-1%; and the maggots are dried until the moisture content is 7%-9%.

[0025] The present invention also seeks to protect the use of the diatom rice protein feed or the diatom rice protein feed prepared by the preparation method in animal breeding.

[0026] Based on long-term scientific research activities and exploration, the inventors proposed a method for the transformation and preparation of diatom rice protein "321".

[0027] (1) The main value of the transformation and preparation of diatom rice protein "321".

[0028] First, the "321" diatom rice protein product, which has been transformed into a product, should be recognized for its superior quality to soybean and fishmeal, thus solidifying its position as a feed protein alternative to soybean and fishmeal from the outset. Second, as a new resource, it completely overcomes the long-standing and long-term reliance on international imports, ensuring sufficient quantity to meet demand and provide surplus for emergencies, ensuring reliable application. Furthermore, due to its advanced production process, the product's production cost is 20% to 15% lower than that of soybean and fishmeal. Therefore, it enjoys strong appeal and competitive dynamism in both the domestic and international markets. In particular, the "321" diatom rice protein production process is an ecologically optimized process.

[0029] (2) Basic raw materials for the transformation and preparation of diatom rice protein “321”.

[0030] Level 1: Basic ingredients at the "3" position in "321": glutinous rice, rice, rice bran, sweet wine yeast, shochu yeast, brewer's yeast, dry yeast, fresh malt, diatom powder, and bamboo shoot powder. Level 2: Basic ingredients at the "2" position in "321": Astragalus, licorice, Codonopsis pilosula, Millettia reticulata, Cordyceps sinensis, and Bombyx mori. Level 3: Basic ingredients at the "1" position in "321": cooked rice, fresh papaya juice, walnut powder, baking soda, salt, loach or catfish slime, brown sugar, and banana paste.

[0031] (3) Raw material proportions, steps and methods for the transformation and preparation of diatom rice protein “321”.

[0032] (1) The raw material proportions, steps and methods for preparing diatom rice polysaccharide for the first time.

[0033] (1.1) Raw material share.

[0034] 55-70 parts of glutinous rice, 15-18 parts of rice, 5-7 parts of rice bran, 2-3 parts of sweet wine yeast, 1-2 parts of shochu yeast, 0.5-1 part of brewer's yeast, 0.5-0.7 part of dry yeast powder, 3-5 parts of fresh malt, 2-2.6 parts of diatom powder and 3-4 parts of bamboo powder.

[0035] (1.2) Steps and methods

[0036] (1.2.1) Mix glutinous rice, rice, and rice bran evenly and steam until cooked to obtain material A.

[0037] (1.2.2) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder, and bamboo powder to 100 mesh to obtain material B.

[0038] (1.2.3) Cool material A to 15-18°C, then thoroughly mix with material B, place in a sealed fermentation vat (tank). When the temperature is 18-22°C, seal for 60-72 hours; when the temperature is 23-28°C, seal for 48-60 hours; when the temperature is 28-35°C, seal for 12-24 hours. The rice polysaccharide product is qualified when the polysaccharide content is 25%-34% and the protein content is 12%-17%.

[0039] (2) The raw material proportions, steps and methods for the second preparation of diatom rice multi-bacteria enzyme and protein.

[0040] (2.1) Raw material share

[0041] 0.1-0.3 parts of Xuefeng Cordyceps, 1-1.5 parts of Bombyx mori, 2-2.5 parts of Astragalus, 1-1.8 parts of Licorice, 2-2.3 parts of Codonopsis pilosula, 2.5-3 parts of Millettia reticulata, 3.2-4 parts of Isatis indigotica powder and 4.1-5.2 parts of mulberry leaf powder.

[0042] (2.2)Steps and methods.

[0043] (2.2.1) Remove the rice polysaccharide product from the fermentation tank (barrel) and add powdered astragalus root, licorice root, codonopsis pilosula, and millettia reticulata, crushed to a mesh size of 85 or greater. Mix thoroughly, then seal the tank (barrel) for a second, intensive fermentation. This purifies and rejuvenates the bacteria involved in the fermentation, contributing to the production of higher levels of saccharifying enzymes, amylases, proteases, oxidases, cellulases, and a richer supply of ATP. This fermentation takes 5-7 hours when the temperature in the fermentation tank (barrel) reaches 22-28°C. If the temperature reaches 29-34°C, fermentation only takes 3-5 hours. This is an anaerobic fermentation process. During this process, the rice polysaccharide content must reach 35%-40%, the protein content must reach 18%-25%, and the total amount of vitamins, primarily VB, in the primary feed must be ≥8%-12% to qualify.

[0044] (2.2.2) Pour the rice polysaccharide and protein products obtained from the second strong fermentation from the fermentation tank (barrel), add the isatis leaf powder, mulberry leaf powder, Xuefeng Cordyceps and Bombyx mori respectively in proportion, mix thoroughly, put into the can (barrel) and seal it to a semi-anoxic state, allowing the Xuefeng Cordyceps and Bombyx mori to grow freely. They can grow freely at room temperature of 18-22℃ for 72 hours; freely grow at room temperature of 23-28℃ for 48 hours; and freely grow at room temperature of 29-34℃ for 24 hours. The diatom rice polysaccharide enzyme and protein content of ≥28% and the content of vitamin B as the main component of the primary material ≥15% are qualified.

[0045] (3) The third time, the transformation preparation of diatom rice protein.

[0046] (3.1) Raw material share.

[0047] 60-70 parts of cooked rice, 2-3 parts of fresh papaya juice, 1-2 parts of walnut powder, 1.5-5.2 parts of baking soda, 0.5-1 part of table salt, 1.5-2.0 parts of loach mucus or catfish mucus, 3-4 parts of brown sugar and 4-5 parts of banana paste.

[0048] (3.2)Steps and methods.

[0049] (3.2.1) Weigh the above raw materials according to the proportions.

[0050] (3.2.2) Mix the above ingredients thoroughly and pile them up, seal them at room temperature between 19-35℃ for 24-32 hours, and emit a strong fishy smell.

[0051] (3.23) Unpack the strongly fishy raw materials and move them to an open area with clear skies or an open room protected from rain. Sprinkle 0.5-1 kg of raw materials per square meter per floor to attract flies to feed, mate, and lay eggs. Once approximately 50 flies per square meter per floor have been feeding for 3-4 hours, recycle the scattered raw materials and use them as a nutrient medium for maggot cultivation.

[0052] (3.2.4) The nutrient medium containing fly eggs should be 2-3 inches thick, with a humidity of 70%-80%, a temperature of 25-32°C, and a light-dark brightness. Maggots can generally be harvested after 80-100 hours of growth in the nutrient medium.

[0053] (3.2.5) Scald the harvested maggots in 45-50°C hot water, add 0.8%-1% salt and mix thoroughly, then allow to air dry, or dry to reduce the moisture content to 7%-9% before use.

[0054] The maggot dry powder obtained in this way has a protein content of 56%-64% and is qualified. At the same time, it contains rich mineral elements, vitamins, and various enzymes.

[0055] Obviously, the so-called "321" transformation method for preparing diatom rice protein means that at the "3" position, the maggots are generally provided with polysaccharide nutrition; at the "2" position, the maggots are generally provided with polysaccharide, protein and microorganism, vitamin and mineral nutrition with improved quantity and quality; at the "1" position, through the supply of "3" and "2" digital nutritional pathways, the maggots can meet their growth and development needs without obstacles, thereby achieving the goal of a gorgeous transformation and producing high-quality protein and other nutrients in high and stable yields.

[0056] (4) The mechanism of the “321” transformation preparation of diatom rice protein.

[0057] The mechanism of the "321" method for the transformation and preparation of diatom rice protein has both extremely rich content and strict numerical limitation ranges. For the three numerical ranges of "321", none can be less, and none can be more. With these three numerical ranges, the transformation and preparation of rice protein can completely have an absolute advantage in terms of output quantity and quality. If there is one more numerical range, it is bound to be a huge waste of resources. On the contrary, there will be a cliff-like decline in the quantity and quality of the output protein, and even denaturation and collapse. If there is one less numerical range, either the "3" numerical range or the "2" numerical range, the so-called transformation cannot be said to have any activity. Therefore, it is very necessary to explain the mechanism clearly:

[0058] (1) The scientific nature and necessity of high-level reverse reasoning.

[0059] Why is plant nutrition placed at the "3" value in the "321" method? Compared to the "2" value and the "1" value, it's undoubtedly a high-level placement. This high-level placement has sound scientific rationale. Specialized nutrients require nutritious material maintenance, and as a foundational maintenance, it must be placed at the top. Only by prioritizing foundational maintenance can we even consider the possibility of transformative protein production. Within this foundational maintenance, prioritizing both plant and microbial nutrition is crucial, with a combined approach being optimal. Clearly, due to the limited activity of plant nutrients, the increased emphasis on microbial nutrition and cultivation has naturally led to significant momentum and success. Generally speaking, at the "2" value, a 10%-20% increase in microbial populations will result in a 40%-60% increase in maggot production, and a 50%-70% increase in the total nutritional content of insect protein. This positive correlation between quantity and quality is an inherent reflection of its scientific mechanism. Further implementation of the foundational work for transformative diatom rice protein production is undeniable.

[0060] (2) The advancement and rationality of median backward reasoning.

[0061] The value "2" in the "321" method is in the middle. The purpose of reversing the value "2" is to serve the value "1," and therefore, its role as the backbone is crucial. First, the value "2" is fully capable of serving as the backbone because it thrives and produces abundantly, thanks to the polysaccharides provided by the value "3." Second, the value "2"'s role as the backbone has its own unique characteristics. Due to the action of polysaccharides, microorganisms are purified and rejuvenated through fermentation. On this basis, the introduction of traditional Chinese medicine active agents to promote the unimpeded growth and development of large-scale microorganisms generally leads to five technological developments in microbial cultivation: ① The promotion of traditional Chinese medicine can shift microorganisms from exponential growth to geometric growth, with a dramatic increase in growth volume and growth time. ② The promotion of traditional Chinese medicine can enable microbial species, such as yeast, probiotics, protein bacteria, and saccharifying enzyme bacteria, to thrive in harmony, interdependently enhancing their vitality. ③ The enzymes, growth hormones, vitamins, mineral elements, and nutrients such as proteins, fats, and carbohydrates produced by the microorganisms have all seen a double increase in both quantity and quality. ④ At this stage, it already functions as a rice polysaccharide feed, rice protein feed, microbial feed, silicon, polysaccharide oxidant, and future high-quality feed ingredient. ⑤ It is an open-loop, publicly accessible high-protein feed ingredient. Its open-loop nature allows it to accommodate other beneficial microorganisms. Its openness provides a completely new approach to transformative protein production.

[0062] Therefore, when the "321" method transitioned to producing diatom rice protein at this stage, a completely new selection method, leveraging the lens of "openness," was inevitably employed. This is because the method of this invention was inherently highly selective. Diatomaceous earth and bamboo powder were chosen because they were intended to "work" for the microorganisms. Diatomaceous earth and bamboo powder are legal feed ingredients. Furthermore, the porous nature of silica and the large surface area of ​​bamboo powder provide a storage and supply mechanism for microbial nutrient production. Bamboo powder also provides a niche for cellulolytic enzymes. Consequently, silica and bamboo powder can absorb large quantities of nutrients, stimulating microorganisms to accelerate production, nutrient decomposition, purification, and growth. Clearly, targeted selection is conducted on top of general selection, followed by specific selection. The second step in preparing diatom polymicrobial culture involves targeted selection. This targeted selection is also divided into three stages. The first stage involves the addition of traditional Chinese medicine to strengthen the bacterial colony and enhance its protein-producing capacity. The second stage involves the addition of Xuefeng Cordyceps sinensis. Xuefeng Cordyceps is a new species discovered in Dongkou County, Hunan Province, China. It has been proven safe and non-toxic through pilot and pilot tests, as well as acute toxicity, reproductive toxicity, and long-term toxicity tests. Its bacterial colonies contain over 65% protein and are rich in minerals and nutrients, including mannitol. It possesses six key benefits: ① Anti-influenza virus; ② Kidney-tonifying and yang-strengthening properties; ③ Enhanced immune function; ④ Anti-tumor; ⑤ Anti-aging and beauty benefits; ⑥ Anti-fatigue and enhanced physical fitness. In recognition of Xuefeng Cordyceps' numerous benefits, the inventors, after hundreds of experiments, successfully developed a method for artificially preparing it in 2013 and obtained a patent for the invention. This time, they selected it for a multi-strain preparation, aiming to fully utilize its functions and cultivate the corresponding bacterial colonies. However, given its limited availability, a small number of strains were used for maximum cultivation. Furthermore, the Xuefeng Cordyceps fungus was used to infect silkworms, resulting in a 99.2% genetic identity between the infected silkworm colonies and the Xuefeng Cordyceps fungus. Therefore, utilizing Bombyx mori can alleviate resource constraints while significantly reducing resource costs. Furthermore, both Xuefeng Cordyceps and Bombyx mori complement previously used yeasts, probiotics, and proteases without conflicting or contradicting each other. They complement each other's strengths, coexisting with each other, developing in the same direction, and achieving similar effects. Therefore, choosing a targeted approach means setting standards, determining benefits, and ultimately, determining highlights and safety. It involves identifying breakthroughs in environmental protection and prioritizing the advancement and rationality of the production process. The cultivation of Xuefeng Cordyceps and the addition of Isatis indigotica powder, as well as the cultivation of Bombyx mori and the addition of mulberry leaf powder, not only boosted the colony size but also increased protein content by 3%-5%, achieving significant improvements in properties. This has particular value in promoting animal growth, enhancing immune function, and strengthening plastid health, particularly laying a unique foundation for the next phase of transformation into high-protein products.

[0063] (3) The uniqueness and subversiveness of the sovereign status transformation.

[0064] The aforementioned "3" and "2" preparations are all foundational, designed to align around the dominant position of transformational preparation. They generally exhibit five key characteristics: First, they transcend species, switching from microbial to insect selection. Second, they transcend protein sources, shifting from existing microbial protein raw materials to producing maggot protein products. Third, they transcend process. Microbial protein production processes differ significantly from maggot protein production processes. Fourth, they transcend disciplines and sectors. Fifth, they transcend perceived product portfolios. Therefore, they possess unique status and disruptive value. This uniqueness is demonstrated in five aspects: 1. Enhanced product value orientation. Once the product process operations for "1" are completed, the "321" approach can be fully implemented. Undoubtedly, the value of each numerical value is fully realized, fully utilized, and fully scientifically validated. This comprehensive demonstration demonstrates that, at the initial design stage, there was concern about whether silica, with its strong sterilizing properties, would negatively impact the bacterial colonies in the raw material. However, testing has proven that diatoms and silica proteins have a 100% affinity for bacterial colonies. Rice polysaccharides, in particular, possess a unique ability to oxidize and dissolve silicon, achieving a 100% safety factor. Furthermore, Xuefeng Cordyceps and silkworm fungi derived from this fungus also exhibit 100% compatibility. This achieves the ideal goal of comprehensive nutrition, product, and process integration. ② Greater product production momentum. This protein production plan was inspired by numerous trials, during which deficiencies were overcome, errors corrected, and gaps filled. Ultimately, it has developed a standardized, uniquely valuable, and fully integrated process, ensuring production safety, quality reliability, and continuity. ③ Enhanced product functionality. Rice protein products transformed under the "321" law are representative products with exceptional functionality. Especially when loach or catfish mucus is used to lure flies to lay eggs and cultivate maggots, the resulting protein products offer exceptionally high protein nutrition and comprehensive antibacterial properties. The resulting maggot oil and fatty acids are rich in added value, many times greater than gold. These products play an indispensable and irreplaceable role in cancer prevention and immune enhancement. (④) Enhanced product culture. New protein resources are being sought, particularly insect farming as a key area of ​​focus. Among these, the development and utilization of fly resources is particularly noteworthy. Flies and their maggots have become a breakthrough in developing new, high-quality protein resources. Maggots reproduce quickly, have a short breeding cycle, are easy to raise, and are readily available, making them a readily exploitable insect protein. Maggot powder is high in protein and rich in amino acids, vitamins, and minerals. Dried maggot powder contains 59%-65% crude protein, 12% fat, 7% ash, 3.1% carbohydrates, and 43.83% total amino acids—3.3 times that of fish meal. It also contains active substances such as antimicrobial peptides and clusterin, boasting comprehensive nutritional benefits, superior to protein resources from meat and eggs, and unparalleled development potential... These advanced cultural elements will inevitably establish a key industrial chain for contemporary ecological agriculture. ⑤ Exploring product potential is even more essential.To date, the maggot protein production chain remains short and fragile, but its potential is enormous. Some scholars have asserted that if my country utilized 1% of its current population's living space, and if each household devoted 1 / 24 of their time daily to raising maggots, the production of high-quality insect protein could increase 100%, making the country the richest in protein resources in the world. Therefore, while fully acknowledging the unique value of this transformation, we must also fully recognize its disruptive significance: ① The diatom rice protein production technology and process have revolutionized the very concepts of agriculture and industry. ② The resulting protein products and their abundant resources have revolutionized the concepts of modern nutrition and medicine. ③ The high-value and expensive products derived from maggots, such as maggot oil, shells, and chitosan, have overturned the scientific understanding of the sacredness and mystery of the unknown world. ④ With the launch of products such as maggot oil, chitin, chitosan and antimicrobial peptides prepared from maggots, they will enrich people's daily lives and have huge economic and social benefits. This will overturn the long-standing market rhythm of low agricultural added value and open up a new era in which agriculture leads and sets a benchmark in all walks of life.

[0065] (6) The main process for the transformation and preparation of diatom rice protein.

[0066] (6.1) Take what is and what is not the process.

[0067] The "take what is, not what is" process can create something out of nothing, becoming the initial signal for transformation. In the "321" method for product preparation, the various raw materials in the high digit "3" serve merely as "food" and "warehouses" for the microorganisms. Their production and processing yield highly concentrated polysaccharides. The polysaccharide raw materials in the middle digit "2" are then processed and cultivated to produce highly active microbial colonies and microbial products. Clearly, this embodies the "take what is, not what is" process. The "1" digit below the "2" utilizes microbial colonies and microbial products to transform into a nutrient medium for maggot cultivation. The resulting product is maggot powder protein and other highly active substances, again embodying the "take what is, not what is" process.

[0068] (6.2) Take what is not and then become the process.

[0069] After perfecting the first process of being and not being, the next process of taking the non-being and then being can be developed. In this process, all raw materials used in the production process are not the final product, but through meticulous and precise preparation, they all move closer to the direction and goal of becoming the final product.

[0070] (6.3) Take the yes and no and then the no process.

[0071] In the "321" method, whether preparing polysaccharides, cultivating microorganisms, or ultimately obtaining the proteins produced by microorganisms, especially the transformed maggot protein, the process of taking yes, taking no, taking no, and then taking no again, emphasizes the positive correlation between product quantity and quality and repeated advancement. This can maximize the use of product time and space, make the most timely and best use of it, and ensure that yes and no have the most substantial use value and market price, and stand out in the market commercialization process. Obviously, carrying out the process of taking yes, taking no, and then taking no again is not just a simple product output issue. From the beginning, the attention and foothold must attach importance to the reasonable investment and addition of supporting raw materials to provide material guarantees for better, more, and faster product output.

[0072] (6.4) The transformation process of taking yes and no and then yes again.

[0073] From the outset, the goal of transforming diatom rice protein production was not to achieve prime ministerial status, but rather to achieve the status of a sage. Consequently, the transformational production process, progressing from polysaccharide production to microbial production and ultimately to insect protein production, began and ended with transformation. This transformational process eschews assumptions, arbitrary decisions, or the art of forcing a breakthrough. Instead, it prioritizes exploration and practical action, ensuring that the new products and technologies derived from this transformational process are both contemporary and full of disruptive energy.

[0074] (VII) Collection of three innovative technologies for the transformation and preparation of diatom rice protein

[0075] Among the innovative technologies for the transformation and preparation of diatom rice protein, three types of technologies are particularly needed and important, including cross-border technology for preparing cross-disciplinary products, cross-disciplinary technology for preparing cross-disciplinary products, and process technology for preparing cross-time and space products. Each of them has its own special requirements.

[0076] (1) Cross-border manufacturing technology for products.

[0077] Cross-border technology for the preparation of cross-border products is a foundational technology that provides both a technical foundation and the essential material resources for the products. Furthermore, it accurately and systematically provides a theoretical basis for cross-border product preparation. This is because cross-border technology is both a highly abstracted abstraction of the commonalities and individual characteristics of the products, and a high-level, concrete application of these commonalities and individual characteristics. Here, we propose a cross-border technology based on "five tests and five observations": 1. "Eating" to test polysaccharide activity. Whether it's plants, animals, or microorganisms, all living organisms require metabolism, and "eating" is a fundamental condition for metabolism. Through "eating" tests, we found that yeast, proteobacteria, and other probiotics readily "eat" rice starch, including the selected traditional Chinese medicine powder, in both aerobic and anaerobic environments. They "eat" more vigorously under anaerobic conditions, resulting in more potent polysaccharide production. Therefore, this provides a solid foundation for the communication between the plant and microbial kingdoms. 2. "Oxygen" testing to observe the rise and fall of communities. All polysaccharide-producing microorganisms, regardless of species, require or undergo aerobic respiration. Or anaerobic respiration. Therefore, by properly controlling oxygen, we can determine the rise and fall of microbial communities, and thus, the affinity and integration mechanisms between microorganisms can be generally determined. By following this path, we can induce diverse microbial communities with strong activity and nutrient production. Although different microorganisms have some physiological, psychological, and biological differences, their reliance on oxygen is generally the same. Therefore, testing oxygen to determine the rise and fall of microbial communities, if maintained as a testing standard, will provide a major trend, driving force, and guarantee for product development. ③ Testing light to determine the reaction rate of product activity. Various light sources, light wavelengths, and light intensities have a strong counterbalancing effect on the reaction rate of product activity. When preparing diatom rice protein for transformation, with the exception of sterilizing ultraviolet light, all other light levels can be used at relatively low levels, and the reaction rate of product activity will remain essentially the same. Conversely, under strong light conditions, polysaccharide production, the growth rate of microbial communities, and especially the protein synthesis rate of transformed maggots will all slow down, or even stagnate. This phenomenon allows us to identify "photon" products, which utilize light to enhance, control light to weaken, and direct light to create them. ④ Test the temperature to determine the total amount of the product's core. The production of diatom rice products is not about polysaccharides or microbial communities, but rather about protein. The key to protein as a core extract lies in three aspects: first, a comprehensive range of proteins. These include at least seven types: diatom protein; microbial protein; traditional Chinese medicine polymer protein; loach or catfish mucus protein; starch protein; maggot protein; and bamboo powder protein. Second, high-quality protein. To date, maggot protein is the leading high-quality protein product. This leadership is demonstrated by its high protein content; high protein yield; wide application range; simple protein production process; and renewable and reproducible protein resources. Third, it is a resource that can be deeply understood and applied.To date, our understanding of fly maggots and protein still has many blind spots, even unknown factors. However, while sacred rather than mysterious, the depth, breadth, and enthusiasm of our understanding are constantly advancing, leading to continuous innovation. Needless to say, during the "temperature testing" process, some inferior products can be eliminated. The optimal temperature for producing polysaccharide products is 18-25°C. Below 18°C, output slows; above 25°C, the possibility of sugar converting into alcohol, vinegar, and harmful mold increases. The optimal temperature for cultivating vibrant microorganisms is generally 19-26°C. Temperatures below 18°C ​​or above 27°C are detrimental to microbial growth and production. When cultivating maggots and preparing protein, temperatures above 33-35°C cause the maggots to consume nutrients rapidly, resulting in a decrease in their growth rate and, in particular, poorer nutrient synthesis. When the temperature drops below 18°C, maggots generally delay hatching, and flies will stop mating and laying eggs. ⑤ Validate the results through reverse testing. Reverse testing serves as a basis for confirming or denying the product's viability and is a fundamental measure for improving product quality and efficiency. Reverse testing for the production of diatom rice protein feed can generally be divided into five categories: raw material testing; conditional testing based on water temperature, air quality, and light; biochemical testing; mathematical testing; and misalignment testing.

[0078] Here, using raw material counter-tests as an example, we present the results of six counter-tests: ① Counter-tests in which diatomaceous earth was omitted as the raw material. In the number "3," all other raw materials and their proportions remained unchanged, except for the exclusion of diatomaceous earth. Post-preparation testing revealed that the polysaccharide content was only 15.3%-17.4%, a 9%-7% decrease compared to polysaccharides prepared with diatomaceous earth. This experiment demonstrates that diatomaceous earth plays a crucial role in the formation of rice polysaccharides and is essential, nor should its proportion be reduced. ② Counter-tests in which bamboo powder was omitted as the raw material. In the number "3," all other raw materials and their proportions remained unchanged, except for the exclusion of bamboo powder. Post-preparation testing revealed that the average polysaccharide content in the six test products was only 16.8%-17.9%, a 6.7%-5.8% decrease compared to polysaccharides prepared with bamboo powder. This experiment demonstrates that bamboo powder has a significant effect on the formation of rice polysaccharides and is essential, nor should its proportion be reduced. ③ Counter-tests in which the raw material of the herbal powder was omitted as the raw material. In the number "2," all other ingredients and proportions remained unchanged, except for the herbal powder. Post-production testing revealed that while the microbial community renewal time was one-fifth faster, the total output of vitamins, trace elements, enzymes, and protein was reduced by one-quarter to one-fifth. These experimental results demonstrate that herbal powders are indispensable in the preparation and cultivation of microbial communities, and their proportion should not be reduced. ④ A counter-test was performed without the use of the Xuefeng Cordyceps fungus. In the number "2," all other ingredients and proportions remained unchanged, except for the removal of the Xuefeng Cordyceps fungus. Post-production testing revealed that the amount of snow-white mycelium in the product was generally reduced by 15%-18%. Authoritative experts have confirmed that these snow-white mycelium are parasitic colonies of the Xuefeng Cordyceps fungus, which possess potent antiviral properties and promote the growth and development of plants, animals, and microorganisms. Clearly, this is a crucial value proposition, and Xuefeng Cordyceps is essential for strengthening the microbial community. ⑤ A counter-test was performed without the use of the silkworm fungus. In the digit "2," all other ingredients and their proportions remained unchanged, with the exception of the Bombyx mori fungus. Post-production testing revealed a 4.2%-5.3% decrease in protein content. This result demonstrates that this extremely valuable microbial protein must be preserved, not discarded. Clearly, Bombyx mori fungus must be utilized, not discontinued. ⑥ A counter-test using loach mucus was conducted: After preparation, it was determined that not only was a fish protein missing, but the total amount of flies attracted was reduced by a quarter, the growth of maggots was significantly slowed, and the production of maggot oil and chitin was significantly reduced. This result demonstrates that to successfully cultivate maggots, loach or catfish mucus is essential.

[0079] (2) Disproportionate technology for interdisciplinary product preparation.

[0080] In production practice, diatom rice protein feed has been transformed and produced. To seek scientific support, demonstration, and guidance, it is essential to master interdisciplinary product development techniques. Simply put, this means mastering the art of "transforming something into something else, creating something out of nothing." The process of transforming diatom rice into protein products generally yields high-quality products in three broad categories: rice polysaccharides, microorganisms, and proteins. These three product categories are inherently interdisciplinary. The diversity of products is evident, spanning from plant nutrition to microbial nutrition to insect nutrition, with a vast range of disciplines. Precisely because of these differences, the products themselves are diverse and unique. This logic demonstrates that despite the vast disparities and profound differences between disciplines, they are always governed by laws and regulations. Therefore, the process of achieving what is, what is not, and what is again is logically seamless. Therefore, products produced with interdisciplinary guidance can also achieve seamless integration.

[0081] (3) Inter-process technology for preparing products across time and space.

[0082] The innovative technologies for transformative diatom rice protein production ultimately converge on time and embrace the considerations of both time and space. In other words, only when the span of time is shortened by space, and the span of space is extended by time, can the innovative technologies for transformative diatom rice protein production gain a foothold and achieve sustainable development. In a product imbued with spirituality, when time aligns with space, time becomes relatively static, and the load on space decreases. When the span of space is affected by the extension of time, the combined force of time and space increases, which in turn broadens the avenues for improving the quality and efficiency of transformative diatom rice protein production, and the potential for future development is already here!

[0083] It can be seen from the above technical solution that compared with the prior art, the beneficial effects of the present invention are as follows:

[0084] The diatom rice protein feed of the present invention is rich in nutrition, high in quality, simple in process, low in raw material cost, production cost and energy consumption cost, has wide application paths and promising market prospects. DETAILED DESCRIPTION

[0085] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0086] Example 1

[0087] Diatom rice protein feed, including component A, component B and component C;

[0088] Component A includes the following raw materials by weight: 55 kg glutinous rice, 15 kg rice, 5 kg rice bran, 2 kg sweet wine koji, 1 kg shochu koji, 0.5 kg brewer's yeast, 0.5 kg dry yeast powder, 3 kg fresh malt, 2 kg diatom powder, and 3 g bamboo powder;

[0089] Component B includes the following raw materials by weight: 0.1 kg of Xuefeng Cordyceps, 1 kg of Bombyx mori, 2 kg of Astragalus, 1 kg of Licorice, 2 kg of Codonopsis, 2.5 kg of Millettia reticulata, 3.2 kg of Isatis indigotica powder, and 4.1 kg of mulberry leaf powder;

[0090] Component C includes the following raw materials by weight: 60 kg of cooked rice, 2 kg of fresh papaya juice, 1 kg of walnut powder, 1.5 kg of baking soda, 0.5 kg of salt, 1.5 kg of loach mucus, 3 kg of brown sugar, and 4 kg of banana paste;

[0091] The preparation method of the above-mentioned diatom rice protein feed specifically comprises the following steps:

[0092] (1) weighing each raw material by the weight of the above-mentioned diatom rice protein feed;

[0093] (2) Mix glutinous rice, rice and rice bran evenly, steam until cooked, and cool to 15° C. to obtain material A, which is then set aside;

[0094] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to 100 mesh to obtain material B;

[0095] (4) Material A and material B were fully mixed and sealed at 35° C. and fermented for 12 h to obtain a diatom rice polysaccharide product;

[0096] (5) Astragalus, licorice, Codonopsis pilosula and Millettia reticulata are mixed, crushed and sieved, and added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented at 34° C. for 3 h to obtain the diatom rice polysaccharide and protein product;

[0097] (6) mixing the isatis leaf powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, and adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting at 34° C. for 24 hours to obtain the diatom rice polysaccharide and protein product;

[0098] (7) Rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus, brown sugar, and banana paste are mixed and added to the diatom rice polymyxin and protein product, stirred evenly, and then sealed and fermented at 35° C. for 24 hours. After spreading, flies are lured to come and eat, mate, and lay eggs for 4 hours. Maggots are cultivated under the conditions of 70% humidity and 32° C. for 100 hours, the maggots are scalded to death, 1% salt is added, and after fully mixing, the mixture is dried until the moisture content of the maggots is 7%, thereby obtaining a diatom rice protein feed.

[0099] Example 2

[0100] Diatom rice protein feed, including component A, component B and component C;

[0101] Component A includes the following raw materials by weight: 70 kg glutinous rice, 18 kg rice, 7 kg rice bran, 3 kg sweet wine koji, 2 kg shochu koji, 1 kg brewer's yeast, 0.7 kg dry yeast powder, 5 kg fresh malt, 2.6 kg diatom powder, and 4 kg bamboo powder;

[0102] Component B includes the following raw materials by weight: 0.3 kg of Xuefeng Cordyceps, 1.5 kg of Bombyx mori, 2.5 kg of Astragalus, 1.8 kg of Licorice, 2.3 kg of Codonopsis, 3 kg of Millettia reticulata, 4 kg of Isatis indigotica powder, and 5.2 kg of mulberry leaf powder;

[0103] Component C includes the following raw materials by weight: 70 kg of cooked rice, 3 kg of fresh papaya juice, 2 kg of walnut powder, 5.2 kg of baking soda, 1 kg of salt, 2.0 kg of loach mucus, 4 kg of brown sugar, and 5 kg of banana paste;

[0104] The preparation method of the above-mentioned diatom rice protein feed specifically comprises the following steps:

[0105] (1) weighing each raw material by the weight of the above-mentioned diatom rice protein feed;

[0106] (2) Mix glutinous rice, rice and rice bran evenly, steam until cooked, and cool to 15° C. to obtain material A, which is then set aside;

[0107] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to 100 mesh to obtain material B;

[0108] (4) Material A and material B were fully mixed and sealed at 35° C. and fermented for 12 h to obtain a diatom rice polysaccharide product;

[0109] (5) Astragalus, licorice, Codonopsis pilosula and Millettia reticulata are mixed, crushed and sieved, and added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented at 34° C. for 3 h to obtain the diatom rice polysaccharide and protein product;

[0110] (6) mixing the isatis leaf powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, and adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting at 34° C. for 24 hours to obtain the diatom rice polysaccharide and protein product;

[0111] (7) Rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus, brown sugar, and banana paste are mixed and added to the diatom rice polymyxin and protein product, stirred evenly, and then sealed and fermented at 35° C. for 24 hours. After spreading, flies are lured to come and eat, mate, and lay eggs for 4 hours. Maggots are cultivated under the conditions of 70% humidity and 32° C. for 100 hours, the maggots are scalded to death, 1% salt is added, and after fully mixing, the mixture is dried until the moisture content of the maggots is 7%, thereby obtaining a diatom rice protein feed.

[0112] Example 3

[0113] Diatom rice protein feed, including component A, component B and component C;

[0114] Component A includes the following raw materials by weight: 55 kg glutinous rice, 15 kg rice, 5 kg rice bran, 2 kg sweet wine koji, 1 kg shochu koji, 0.5 kg brewer's yeast, 0.5 kg dry yeast powder, 3 kg fresh malt, 2 kg diatom powder, and 3 g bamboo powder;

[0115] Component B includes the following raw materials by weight: 0.3 kg of Xuefeng Cordyceps, 1.5 kg of Bombyx mori, 2.5 kg of Astragalus, 1.8 kg of Licorice, 2.3 kg of Codonopsis, 3 kg of Millettia reticulata, 4 kg of Isatis indigotica powder, and 5.2 kg of mulberry leaf powder;

[0116] Component C includes the following raw materials by weight: 60 kg of cooked rice, 2 kg of fresh papaya juice, 1 kg of walnut powder, 1.5 kg of baking soda, 0.5 kg of salt, 1.5 kg of loach mucus, 3 kg of brown sugar, and 4 kg of banana paste;

[0117] The preparation method of the above-mentioned diatom rice protein feed specifically comprises the following steps:

[0118] (1) weighing each raw material by the weight of the above-mentioned diatom rice protein feed;

[0119] (2) Mix glutinous rice, rice and rice bran evenly, steam until cooked, and cool to 15° C. to obtain material A, which is then set aside;

[0120] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to 100 mesh to obtain material B;

[0121] (4) Material A and material B were fully mixed and sealed at 35° C. and fermented for 12 h to obtain a diatom rice polysaccharide product;

[0122] (5) Astragalus, licorice, Codonopsis pilosula and Millettia reticulata are mixed, crushed and sieved, and added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented at 34° C. for 3 h to obtain the diatom rice polysaccharide and protein product;

[0123] (6) mixing the isatis leaf powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, and adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting at 34° C. for 24 hours to obtain the diatom rice polysaccharide and protein product;

[0124] (7) Rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus, brown sugar, and banana paste are mixed and added to the diatom rice polymyxin and protein product, stirred evenly, and then sealed and fermented at 35° C. for 24 hours. After spreading, flies are lured to come and eat, mate, and lay eggs for 4 hours. Maggots are cultivated under the conditions of 70% humidity and 32° C. for 100 hours, the maggots are scalded to death, 1% salt is added, and after fully mixing, the mixture is dried until the moisture content of the maggots is 7%, thereby obtaining a diatom rice protein feed.

[0125] Example 4

[0126] Diatom rice protein feed, including component A, component B and component C;

[0127] Component A includes the following raw materials by weight: 55 kg glutinous rice, 15 kg rice, 5 kg rice bran, 2 kg sweet wine koji, 1 kg shochu koji, 0.5 kg brewer's yeast, 0.5 kg dry yeast powder, 3 kg fresh malt, 2 kg diatom powder, and 3 g bamboo powder;

[0128] Component B includes the following raw materials by weight: 0.1 kg of Xuefeng Cordyceps, 1 kg of Bombyx mori, 2 kg of Astragalus, 1 kg of Licorice, 2 kg of Codonopsis, 2.5 kg of Millettia reticulata, 3.2 kg of Isatis indigotica powder, and 4.1 kg of mulberry leaf powder;

[0129] Component C includes the following raw materials by weight: 70 kg of cooked rice, 3 kg of fresh papaya juice, 2 kg of walnut powder, 5.2 kg of baking soda, 1 kg of salt, 2.0 kg of loach mucus, 4 kg of brown sugar, and 5 kg of banana paste;

[0130] The preparation method of the above-mentioned diatom rice protein feed specifically comprises the following steps:

[0131] (1) weighing each raw material by the weight of the above-mentioned diatom rice protein feed;

[0132] (2) Mix glutinous rice, rice and rice bran evenly, steam until cooked, and cool to 15° C. to obtain material A, which is then set aside;

[0133] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to 100 mesh to obtain material B;

[0134] (4) Material A and material B were fully mixed and sealed at 35° C. and fermented for 12 h to obtain a diatom rice polysaccharide product;

[0135] (5) Astragalus, licorice, Codonopsis pilosula and Millettia reticulata are mixed, crushed and sieved, and added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented at 34° C. for 3 h to obtain the diatom rice polysaccharide and protein product;

[0136] (6) mixing the isatis leaf powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, and adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting at 34° C. for 24 hours to obtain the diatom rice polysaccharide and protein product;

[0137] (7) Rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus, brown sugar, and banana paste are mixed and added to the diatom rice polymyxin and protein product, stirred evenly, and then sealed and fermented at 35° C. for 24 hours. After spreading, flies are lured to come and eat, mate, and lay eggs for 4 hours. Maggots are cultivated under the conditions of 70% humidity and 32° C. for 100 hours, the maggots are scalded to death, 1% salt is added, and after fully mixing, the mixture is dried until the moisture content of the maggots is 7%, thereby obtaining a diatom rice protein feed.

[0138] Example 5

[0139] Diatom rice protein feed, including component A, component B and component C;

[0140] Component A includes the following raw materials by weight: 55 kg glutinous rice, 15 kg rice, 5 kg rice bran, 2 kg sweet wine koji, 1 kg shochu koji, 0.5 kg brewer's yeast, 0.5 kg dry yeast powder, 3 kg fresh malt, 2 kg diatom powder, and 3 g bamboo powder;

[0141] Component B includes the following raw materials by weight: 0.3 kg of Xuefeng Cordyceps, 1.5 kg of Bombyx mori, 2.5 kg of Astragalus, 1.8 kg of Licorice, 2.3 kg of Codonopsis, 3 kg of Millettia reticulata, 4 kg of Isatis indigotica powder, and 5.2 kg of mulberry leaf powder;

[0142] Component C includes the following raw materials by weight: 70 kg of cooked rice, 3 kg of fresh papaya juice, 2 kg of walnut powder, 5.2 kg of baking soda, 1 kg of salt, 2.0 kg of loach mucus, 4 kg of brown sugar, and 5 kg of banana paste;

[0143] The preparation method of the above-mentioned diatom rice protein feed specifically comprises the following steps:

[0144] (1) weighing each raw material by the weight of the above-mentioned diatom rice protein feed;

[0145] (2) Mix glutinous rice, rice and rice bran evenly, steam until cooked, and cool to 15° C. to obtain material A, which is then set aside;

[0146] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to 100 mesh to obtain material B;

[0147] (4) Material A and material B were fully mixed and sealed at 35° C. and fermented for 12 h to obtain a diatom rice polysaccharide product;

[0148] (5) Astragalus, licorice, Codonopsis pilosula and Millettia reticulata are mixed, crushed and sieved, and added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented at 34° C. for 3 h to obtain the diatom rice polysaccharide and protein product;

[0149] (6) mixing the isatis leaf powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, and adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting at 34° C. for 24 hours to obtain the diatom rice polysaccharide and protein product;

[0150] (7) Rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus, brown sugar, and banana paste are mixed and added to the diatom rice polymyxin and protein product, stirred evenly, and then sealed and fermented at 35° C. for 24 hours. After spreading, flies are lured to come and eat, mate, and lay eggs for 4 hours. Maggots are cultivated under the conditions of 70% humidity and 32° C. for 100 hours, the maggots are scalded to death, 1% salt is added, and after fully mixing, the mixture is dried until the moisture content of the maggots is 7%, thereby obtaining a diatom rice protein feed.

[0151] Example 6

[0152] Diatom rice protein feed, including component A, component B and component C;

[0153] Component A includes the following raw materials by weight: 70 kg glutinous rice, 18 kg rice, 7 kg rice bran, 3 kg sweet wine koji, 2 kg shochu koji, 1 kg brewer's yeast, 0.7 kg dry yeast powder, 5 kg fresh malt, 2.6 kg diatom powder, and 4 kg bamboo powder;

[0154] Component B includes the following raw materials by weight: 0.1 kg of Xuefeng Cordyceps, 1 kg of Bombyx mori, 2 kg of Astragalus, 1 kg of Licorice, 2 kg of Codonopsis, 2.5 kg of Millettia reticulata, 3.2 kg of Isatis indigotica powder, and 4.1 kg of mulberry leaf powder;

[0155] Component C includes the following raw materials by weight: 60 kg of cooked rice, 2 kg of fresh papaya juice, 1 kg of walnut powder, 1.5 kg of baking soda, 0.5 kg of salt, 1.5 kg of loach mucus, 3 kg of brown sugar, and 4 kg of banana paste;

[0156] The preparation method of the above-mentioned diatom rice protein feed specifically comprises the following steps:

[0157] (1) weighing each raw material by the weight of the above-mentioned diatom rice protein feed;

[0158] (2) Mix glutinous rice, rice and rice bran evenly, steam until cooked, and cool to 15° C. to obtain material A, which is then set aside;

[0159] (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to 100 mesh to obtain material B;

[0160] (4) Material A and material B were fully mixed and sealed at 35° C. and fermented for 12 h to obtain a diatom rice polysaccharide product;

[0161] (5) Astragalus, licorice, Codonopsis pilosula and Millettia reticulata are mixed, crushed and sieved, and added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented at 34° C. for 3 h to obtain the diatom rice polysaccharide and protein product;

[0162] (6) mixing the isatis leaf powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, and adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting at 34° C. for 24 hours to obtain the diatom rice polysaccharide and protein product;

[0163] (7) Rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus, brown sugar, and banana paste are mixed and added to the diatom rice polymyxin and protein product, stirred evenly, and then sealed and fermented at 35° C. for 24 hours. After spreading, flies are lured to come and eat, mate, and lay eggs for 4 hours. Maggots are cultivated under the conditions of 70% humidity and 32° C. for 100 hours, the maggots are scalded to death, 1% salt is added, and after fully mixing, the mixture is dried until the moisture content of the maggots is 7%, thereby obtaining a diatom rice protein feed.

[0164] Performance Testing

[0165] The diatom rice protein feeds prepared in Examples 1-6 were fed to pigs, beef cattle, Leghorn chickens, Matou goats, Beijing ducks, and freshwater fish, respectively. A commercially available feed for the corresponding animals served as a control group. Except for the different feeds, all other farming methods were the same.

[0166] The results are shown in Table 1-6.

[0167] Table 1 Six groups of pig comparative breeding experiments

[0168]

[0169] Table 2 Four groups of comparative experiments on fattening of beef cattle

[0170]

[0171] Note: The Inner Mongolia hybrid cattle test group and control group were randomly sampled and the weights of each group were basically the same.

[0172] Table 3 Five groups of comparative test on returning juvenile chickens to young in the past three years of laying

[0173]

[0174]

[0175] Table 4 Three groups of comparative test on farrowing of Matou female goats

[0176]

[0177] Table 5 Four groups of comparative test on Beijing meat duck breeding

[0178]

[0179] Note: When the ducklings were brought into the breeding farm, the average weight of each duck in the experimental and control groups was 308 grams.

[0180] Table 6 Three groups of comparative experiments on bulk freshwater fish farming

[0181]

[0182] Clearly, the six validation trials above demonstrate that the transformed diatom rice protein has significant potential in the aquaculture industry. This has been fully demonstrated in at least seven areas:

[0183] (1) Whether it is rice polysaccharides, cultivated microorganisms and their products, especially maggot protein powder, it is very safe to use in modern aquaculture.

[0184] (2) The nutritional value of the product is outstanding.

[0185] (3) The product has good environmental protection function, without any wastewater, exhaust gas or dust pollution, and the entire production process is low-carbon, green and environmentally friendly.

[0186] (4) The product has a wide range of applications in modern aquaculture and has a promising market prospect.

[0187] (5) The product is of high quality and the process is simple.

[0188] (6) The raw material cost, production cost and energy consumption cost of the product are low and resources are abundant.

[0189] (7) All resources used in the long term by the product are renewable, can be expanded and reproduced, and can tap into greater potential.

[0190] It can be seen that diatom rice protein transformation preparation technology and products will inevitably occupy a reliable place in the modern and future aquaculture industry.

[0191] The above description of the disclosed embodiments is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A diatom rice protein feed, characterized in that: Comprising component A, component B and component C; The component A comprises the following raw materials in parts by weight: 55-70 parts of glutinous rice, 15-18 parts of rice, 5-7 parts of rice bran, 2-3 parts of sweet wine yeast, 1-2 parts of shochu yeast, 0.5-1 part of brewer's yeast, 0.5-0.7 part of dry yeast powder, 3-5 parts of fresh malt, 2-2.6 parts of diatom powder and 3-4 parts of bamboo powder; The component B comprises the following raw materials in parts by weight: 0.1-0.3 parts of Xuefeng Cordyceps, 1-1.5 parts of Bombyx mori, 2-2.5 parts of Astragalus, 1-1.8 parts of Licorice, 2-2.3 parts of Codonopsis pilosula, 2.5-3 parts of Millettia reticulata, 3.2-4 parts of Isatis indigotica powder, and 4.1-5.2 parts of mulberry leaf powder; The component C comprises the following raw materials in parts by weight: 60-70 parts of cooked rice, 2-3 parts of fresh papaya juice, 1-2 parts of walnut powder, 1.5-5.2 parts of edible baking soda, 0.5-1 part of salt, 1.5-2.0 parts of loach mucus or catfish mucus, 3-4 parts of brown sugar and 4-5 parts of banana paste.

2. A method for preparing a diatom rice protein feed, characterized in that: The specific steps include: (1) weighing each raw material by the weight parts of the diatom rice protein feed according to claim 1; (2) Mix glutinous rice, rice and rice bran evenly, steam them, and cool them to obtain material A, which is then set aside; (3) Mix and grind sweet wine koji, shochu koji, brewer's yeast, dry yeast powder, fresh malt, diatom powder and bamboo powder to obtain material B; (4) After fully mixing material A and material B, sealing and fermenting to obtain a diatom rice polysaccharide product; (5) Astragalus, Licorice, Codonopsis pilosula and Millettia repens are mixed and crushed and sieved, and then added to the diatom rice polysaccharide product. After mixing evenly, the mixture is sealed and fermented to obtain the diatom rice polysaccharide and protein product; (6) mixing Folium Isatidis powder, mulberry leaf powder, Cordyceps sinensis and Bombyx mori, adding the mixture to the diatom rice polysaccharide and protein product, stirring evenly and then semi-sealing and fermenting to obtain the diatom rice polysaccharide and protein product; (7) After rice, fresh papaya juice, walnut powder, baking soda, salt, loach mucus or catfish mucus, brown sugar, and banana paste are mixed, added to the diatom rice multibacterial enzyme and the protein product, stirred evenly, and then sealed and fermented. After spreading, flies are lured to come and eat, mate, and lay eggs, maggots are cultivated, and the maggots are scalded to death. Salt is added, and the mixture is fully stirred and then naturally air-dried or dried to obtain the diatom rice protein feed.

3. A diatom rice protein feed according to claim 2, characterized in that, In step (2), the temperature is cooled to 15-18°C.

4. A diatom rice protein feed according to claim 2, characterized in that, In step (3), the powder is ground into 100 mesh.

5. A diatom rice protein feed according to claim 2, characterized in that, In step (4), the temperature of the sealed fermentation is 18-35° C., and the time is 12-72 hours.

6. A diatom rice protein feed according to claim 2, characterized in that, In step (5), the temperature of the sealed fermentation is 22-34° C., and the time is 3-7 hours.

7. A diatom rice protein feed according to claim 2, characterized in that, In step (6), the temperature of the semi-sealed fermentation is 18-34° C., and the time is 24-72 hours.

8. A diatom rice protein feed according to claim 2, characterized in that, In step (7), the temperature of the sealed fermentation is 19-35° C., and the time is 24-32 hours; the time of feeding, mating, and laying eggs is 3-4 hours; and the humidity of the maggots is 70%-80%, the temperature is 25-32° C., and the time is 80-100 hours.

9. A diatom rice protein feed according to claim 2, characterized in that, In step (7), the mass of the added salt is 0.8%-1%; and the moisture content of the maggots after drying is 7%-9%.

10. Use of the diatom rice protein feed according to claim 1 or the diatom rice protein feed prepared by the preparation method according to any one of claims 2 to 9 in animal breeding.

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