Skin-care combined peptide and preparation method thereof

Abstract

The invention belongs to the field of small molecule peptides, and particularly relates to a skincare combined peptide and a preparation method thereof, and the combined peptide contains palmitoyl tripeptide-5, palmitoyl tetrapeptide-7 and a solvent component. The combined peptide obtained by the invention has excellent anti-aging and anti-wrinkle effects, is simple in components, safe and non-irritant, and has a very good application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of small molecule peptides, and in particular relates to a skin care combination peptide and a preparation method thereof. Background Art

[0002] Peptides are a class of small molecule compounds formed by the dehydration condensation reaction of multiple amino acids. Peptides with fewer than 50 amino acid residues are usually called polypeptides, while peptides with more than 50 amino acid residues are called macromolecular proteins. With the continuous development of modern biotechnology and chemical synthesis technology, new bioactive peptides and chemically synthesized peptides are constantly being researched and discovered. Most biological reactions and chemical reaction processes in nature are regulated by specific amino acid sequences. By transcribing the interacting peptide sequences, they can be induced to produce small, active and stable fragments. The application of these small fragments in skin cell proliferation and differentiation, extracellular matrix synthesis, pigmentation, etc. has good efficacy, providing good opportunities for the development of dermatology and skin care fields. In addition, peptide drugs have the advantages of small molecular weight, high safety, strong specificity, low toxicity and side effects, and high immunogenicity, becoming a new hotspot in research and development.

[0003] In the current cosmetics market, anti-aging and anti-wrinkle skin care products account for an increasingly higher proportion, and a large number of peptides are added. Consumers expect high-performance skin care products to smooth fine lines and tighten the skin, but are also worried about excessive use causing secondary damage to the skin. Some consumers with sensitive skin are even too worried about the irritation of anti-aging and anti-wrinkle skin care products and are unable to find suitable anti-aging and anti-wrinkle skin care products.

[0004] Therefore, the development of a safe and effective anti-aging and wrinkle-removing polypeptide combination peptide is of great significance for the research and development of a new generation of anti-aging cosmetics. Summary of the Invention

[0005] The present invention aims to overcome the deficiencies of the prior art and provides a skin care combination peptide. The combination peptide has a simple formulation and strong combined effect, can exert a strong anti-aging effect, and effectively reduces process and raw material costs.

[0006] Specifically, the present invention is achieved through the following technologies: A skin care combination peptide, comprising: palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7.

[0007] Preferably, the combination peptide is composed of the following components in parts by weight: 0.05%-0.5% palmitoyl tripeptide-5 and 0.01%-0.1% palmitoyl tetrapeptide-7.

[0008] Further preferably, the combination peptide is composed of the following components in parts by weight: 0.1% of palmitoyl tripeptide-5 and 0.05% of palmitoyl tetrapeptide-7.

[0009] In some embodiments of the present invention, the combination peptide further contains a solvent component, which includes solvents, oils, chelating agents, emulsifiers, moisturizers, thickeners, solubilizers, emollients, regulators, antioxidants, whitening agents, conditioners, soothing agents and natural flavors / pigments.

[0010] In some more preferred embodiments of the present invention, the solvent component includes conventional solvents in the art or mixtures thereof. In an embodiment of the present invention, the solvent component is selected from polyols, preservatives, solubilizers, pH regulators and water.

[0011] Preferably, the polyol is at least one of dipropylene glycol, 1,3-propylene glycol, glycerol, ethylene glycol, 1,2-butylene glycol, butylene glycol, panthenol, 1,2-hexanediol, 1,2-pentanediol, and sodium hyaluronate; Further preferably, the polyol is a mixture of 1,2-hexanediol and sodium hyaluronate; More preferably, the molar ratio of 1,2-hexanediol to sodium hyaluronate is 1:3-5.

[0012] Preferably, the solubilizer is one of polysorbate and poloxamer.

[0013] Preferably, the pH adjuster is at least one of aminomethyl propanol, citric acid, and glycylglycine buffer.

[0014] Preferably, the combination peptide comprises the following components: The present invention provides a method for preparing a combination peptide, which is prepared by the following steps: Weigh the prescribed amount of polyol and solubilizer, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically vibrate, then add palmitoyl tetrapeptide-7, ultrasonically vibrate for 1-5 minutes, add water to make up to volume, add pH regulator to adjust pH, then incubate in a water bath, let stand, and obtain a combined peptide.

[0015] Preferably, the method for preparing the combined peptide comprises the following specific steps: Weigh the prescribed amount of polyol and solubilizer, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically vibrate for 5-10 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically vibrate for 1-5 minutes, add water to make up the volume, add pH regulator to adjust the pH, ultrasonically vibrate for 10-30 minutes, then place in a 40-60 ° C water bath for incubation for 10-60 minutes, and after the incubation, let it stand at 0-25 ° C for 1-12 hours to obtain a combined peptide.

[0016] Beneficial effects of the present invention: The combined peptide obtained by the present invention has excellent anti-aging and wrinkle-removing effects, is simple in composition, and is safe and non-irritating. DETAILED DESCRIPTION

[0017] The present invention will be further described below with reference to specific examples. It should be understood that the following examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. Unless otherwise specified, the materials and raw materials used in the present invention can be obtained through commercial channels.

[0018] Example 1, a skin care combination peptide, containing the following components by weight: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0019] Example 2, a skin care combination peptide, containing the following components by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-20, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 5.0, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0020] Example 3, a skin care combination peptide, containing the following components by weight: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and poloxamer, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically vibrate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically vibrate for 3 minutes, add water to make up to volume, add aminomethyl propanol to adjust the pH to 7.5, ultrasonically vibrate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0021] Example 4, a skin care combination peptide, containing the following components by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0022] Example 5, a skin care combination peptide, containing the following components by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0023] Example 6, a skin care combination peptide, containing the following components in parts by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol and polysorbate-20, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0024] Example 7, a skin care combination peptide, containing the following components by mass: Preparation process: Weigh the prescribed amount of sodium hyaluronate and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up to volume, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0025] Comparative Example 1, a skin care combination peptide, containing the following components by weight: Preparation process: Weigh the prescribed amount of glycerol and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically vibrate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically vibrate for 3 minutes, add water to make up, add citric acid to adjust the pH to 5.5, ultrasonically vibrate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0026] Comparative Example 2, a skin care combination peptide, containing the following components by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-60, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up to volume, add aminomethyl propanol to adjust the pH to 8.0, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0027] Comparative Example 3, a skin care combination peptide, containing the following components by mass: Preparation process: Weigh the prescribed amount of 1,2-butanediol, sodium hyaluronate and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 4.0, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath and incubate for 30 minutes. After incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0028] Comparative Example 4, a skin care combination peptide, contains the following components in parts by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate, polysorbate-80, palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7, add water to dissolve, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in a 55°C water bath for incubation for 30 minutes, and let it stand at 25°C for 4 hours to obtain a combined peptide.

[0029] Comparative Example 5, a skin care combination peptide, containing the following components in parts by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-80, add water to dissolve, add palmitoyl tripeptide-5 and ultrasonically oscillate for 8 minutes, then add palmitoyl tetrapeptide-7 and ultrasonically oscillate for 3 minutes, add water to make up the volume, add citric acid to adjust the pH to 5.5, ultrasonically oscillate for 20 minutes, then place in an 80°C water bath and incubate for 30 minutes. After the incubation, let it stand at 25°C for 4 hours to obtain a combined peptide.

[0030] Comparative Example 6, a skin care combination peptide, containing the following components in parts by mass: Preparation process: Weigh the prescribed amount of 1,2-hexanediol, sodium hyaluronate and polysorbate-80, add water to dissolve, add citric acid to adjust the pH to 5.5, then add palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7, ultrasonically oscillate for 20 minutes, and let stand at 25°C for 4 hours to obtain a combined peptide.

[0031] Verify the implementation examples; 1. Cytotoxicity test: Prepare cell suspension: HFF-1 cells, resuspend the cells in PBS, and adjust the cell concentration so that the number of cells per well is 1×10 5 , incubator at 37 ° C, 5% CO2 overnight. Take the combined peptide of Example 1 and dilute it into 4 gradients with 10% FBS + EMEM culture medium, set a 0 concentration point as a blank control, and set three replicates for each concentration point. After the administration is completed, the 96-well plate is placed in an incubator (37 ° C, 5% CO2) and cultured for 24 hours. After the incubation is completed, the supernatant is discarded, MTT working solution is added, and incubated at 37 ° C in the dark for 2 hours. After the incubation is completed, the supernatant is discarded, 100μl DMSO is added to each well, and the OD value is read at 490nm. The results are shown in Table 1. The combined peptide did not show cytotoxicity at a concentration below 0.5%.

[0032] Table 1 Cytotoxicity test results 2. Anti-wrinkle Efficacy Testing: 13 peptide combinations obtained from Examples 1-7 and Comparative Examples 1-6 were tested for anti-wrinkle efficacy. A total of 130 volunteers, 10 in each group, were tested on one peptide combination. After washing their faces, participants were placed in a constant temperature and humidity environment (21-23°C, 55-65%) for 30 minutes. Their faces were then photographed using a VISA-CR. The combined peptides were then applied and gently patted for absorption. The participants continued using the combined extracts for 8 weeks. After the eighth week, their faces were washed and photographed using a VISA-CR. The facial wrinkle improvement rate and R2 improvement rate before and after application were calculated using instrument software to reflect the immediate improvement in facial wrinkles achieved by each peptide combination.

[0033] The results are shown in Table 2. The anti-wrinkle effect of the combined peptides obtained using Examples 1-3 is significantly better than that of Examples 4-7 and Comparative Examples 1-6, and the wrinkle removal rate of Examples 1-3 reaches more than 41%. The anti-wrinkle effect of Examples 1-3 is significantly better than that of Examples 4-7 and Comparative Examples 1-6, indicating that the combined peptides prepared according to the present invention have good anti-wrinkle effects.

[0034] Table 2 Anti-wrinkle effect .

Claims

1. A skin care combination peptide, characterized in that, The combination peptide comprises the following components: 0.05%-0.5% Palmitoyl Tripeptide-5, 0.01%-0.1% Palmitoyl Tetrapeptide-7.

2. The combination peptide according to claim 1, characterized in that The composition comprises the following components: 0.1% Palmitoyl Tripeptide-5, 0.05% Palmitoyl Tetrapeptide-7.

3. The combination peptide according to claim 1, characterized in that The combination peptide further contains a solvent component, which is selected from polyols, preservatives, solubilizers, pH regulators and water.

4. The combination peptide according to claim 1, characterized in that The polyol is at least one of dipropylene glycol, 1,3-propylene glycol, glycerol, ethylene glycol, 1,2-butylene glycol, butylene glycol, panthenol, 1,2-hexanediol, 1,2-pentanediol, and sodium hyaluronate; the solubilizer is one of polysorbate and poloxamer; and the pH adjuster is at least one of aminomethyl propanol, citric acid, and glycylglycine buffer.

5. The combination peptide according to claim 1, characterized in that The polyol is a mixture of 1,2-hexanediol and sodium hyaluronate.

6. The combination peptide according to claim 5, characterized in that The molar ratio of 1,2-hexanediol to sodium hyaluronate is 1:3-5.

7. The combination peptide according to claim 1, characterized in that The combination peptide comprises the following components: 。 8. A method for preparing the combination peptide according to claim 1, characterized in that: Prepared by the following steps: Weigh the prescribed amount of polyol and solubilizer, add water to mix, add pH regulator to adjust pH, then add palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7, ultrasonically oscillate, then incubate in a water bath, and let stand to obtain a combined peptide.

9. The preparation method according to claim 8, characterized in that: The specific steps are as follows: Weigh the prescribed amount of polyol and solubilizer, add water to mix, add pH regulator to adjust the pH to 5.0-7.5, then add palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7, ultrasonically oscillate for 10-30 minutes, then place in a 40-60°C water bath for incubation for 10-60 minutes, and stand at 0-25°C for 1-12 hours to obtain a combined peptide.

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