Regulation and control system and method for living tissue cells

Abstract

The invention discloses a regulation and control system and method for living tissue cells, relates to the field of biomedicine, can regulate and control specific cells in a deep area to be regulated and controlled in living tissue, and comprises a laser source module, a modulation module, an optical fiber module, an imaging module and a control module, the modulation module modulates the received first laser based on a preset modulation strategy to determine regulation and control light, and modulates the received second laser based on the preset modulation strategy to determine imaging light, the first laser is used for regulating and controlling target cells based on optogenetics, the second laser is used for imaging a target area, and the imaging light is used for imaging the target area. The target area refers to a to-be-regulated area in a living body tissue where target cells are located; the regulation and control light or the imaging light is output to a deep layer area of a living body tissue through a multimode optical fiber in the optical fiber module, the system firstly images a target area through the imaging light, determines a target regulation and control area where a target cell is located, and then precisely regulates and controls the target cell through the regulation and control light.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to a control system and method for living tissue cells. Background Art

[0002] Optogenetics is the process of expressing light-sensitive proteins in specific cells (such as neurons) and then regulating the activity of these specific cells through light of different wavelengths.

[0003] In the biomedical field, living tissues where specific cells are located scatter and absorb light, which affects the propagation of light within the living tissue and limits the penetration depth of light within the living tissue. In this case, how to achieve optogenetic control in the deep area of ​​living tissue is an urgent problem to be solved. Summary of the Invention

[0004] The present application provides a regulation system and method for living tissue cells, which can realize optogenetic control of deep areas in living tissue.

[0005] In a first aspect, a control system for living tissue cells is provided, the system comprising a laser source module, a modulation module, an optical fiber module, an imaging module and a control module, wherein the control module is communicatively connected to the imaging module.

[0006] The laser source module is used to generate a first laser and a second laser, the modulation module is used to modulate the received first laser based on a preset modulation strategy to determine the control light, and modulate the received second laser based on the preset modulation strategy to determine the imaging light, wherein the preset modulation strategy is a strategy for wavefront modulation of the first laser and the second laser; the optical fiber module is used to output the control light or the imaging light, the imaging module is used to receive, through the optical fiber module, a fluorescence signal emitted by the target area after being irradiated by the imaging light, and determine a detection image corresponding to the target area based on the fluorescence signal; the control module is used to determine the target control area where the target cell is located based on the detection image, so that the target cell can be controlled by the control light in the target control area.

[0007] It should be understood that the first laser is used to regulate the target cells based on optogenetics, and the second laser is used to image the target area. Similarly, after modulation, the regulating light is also used to regulate the target cells, and the imaging light is also used to image the target area. The target area refers to the area to be regulated in the living tissue where the target cells are located. Therefore, the target regulating area where the target cells are located belongs to the area to be regulated.

[0008] In some embodiments, the region to be regulated in a living tissue may be located in a superficial region of the living tissue or in a deep region of the living tissue. For example, for the living tissue of the brain, the region to be regulated may be a deep brain region.

[0009] That is, the modulation light or imaging light after the modulation of the first laser and the second laser by the modulation module can be outputted through the optical fiber module, and the detection image corresponding to the target area is determined by the imaging module, thereby visualizing the target area and facilitating the positioning of the target cell in the target area. Furthermore, the target modulation area where the target cell is located is determined in the detection image, which can accurately locate the target cell and facilitate precise control of the target cell. It should be understood that the target cell can be a single cell.

[0010] In one possible implementation, the modulation module includes a dimming unit and a modulation unit; the dimming unit is used to expand or scale the process light passing through the modulation module, and the process light includes at least a first laser and a second laser; the modulation unit is used to adjust the phase and intensity of the process light based on a preset modulation strategy.

[0011] In some embodiments, adjusting the phase and intensity of the process light can be achieved by the same optical element.

[0012] The modulation module realizes wavefront modulation through a preset modulation strategy, and performs high-speed, diffraction-limited focusing through optical fibers to achieve precise positioning of target cells.

[0013] In this case, in one possible implementation, the dimming unit includes a first lens assembly and a second lens assembly, and the modulation unit includes a holographic modulation assembly; the first lens assembly is used to expand the first laser and the second laser; the holographic modulation assembly is used to adjust the phase and intensity of the expanded first laser and the second laser based on a preset modulation strategy to obtain the first output light and the second output light; the second lens assembly is used to scale the first output light and the second output light to determine the controlled light and the imaging light.

[0014] In some embodiments, the preset modulation strategy adjusts the expanded first laser light and the second laser light by using a preset hologram, and the holographic modulation component adjusts the wavefronts of the first laser light and the second laser light by displaying the preset hologram.

[0015] The above-mentioned combination of the first lens assembly, the holographic modulation assembly and the second lens assembly realizes the wavefront adjustment of the first laser and the second laser, wherein the first laser and the second laser pass through the first lens assembly, the holographic modulation assembly and the second lens assembly in the embodiment of the present application in sequence, and can determine the control light for controlling the target cells and the imaging light for imaging the target area, so that the control light and the imaging light can be output through the same optical fiber module, thereby enabling the system to image the target area, accurately locate the target cells, and control the target cells.

[0016] In another possible implementation, the dimming unit also includes a screening component; the screening component is used to screen the first target order among the diffraction orders corresponding to the first output light, and the first target order among the diffraction orders corresponding to the second output light, the controlled light is the light whose diffraction order in the first output light is the first target order, and the imaging light is the light whose diffraction order in the second output light is the first target order.

[0017] It should be understood that the wavelengths of the first laser and the second laser are different. By adjusting the expanded output light fields of the first laser and the second laser based on the preset hologram, the determined first output light and the second output light are also different. By preset the hologram, the first output light and the second output light can both have target levels that can pass through the screening component, so that the determined control light and imaging light can be output through the same optical fiber module, simplifying the part of the system that is inserted into living tissue.

[0018] In a possible implementation, the laser source module includes a beam splitting unit, which is used to split the second laser into a second signal beam and a reference beam, the second signal beam is used to image the target area, and the reference beam is used to calibrate the second signal beam.

[0019] In one possible implementation, the system also includes a calibration module, which includes a reference unit and a recording unit; the reference unit is used to converge the reference beam to the recording unit; the recording unit is used to obtain a combined beam, and determine a first transmission matrix based on the interference between different lights in the combined beam, wherein the combined beam includes a reference beam and object light, and the object light is the controlled light or imaging light irradiated on the target area.

[0020] In order to make the measured first transmission matrix more accurate, the second laser is split into a second signal beam and a reference beam by a beam splitting unit. The first transmission matrix can be made more accurate based on the interference between the reference beam and the light output from the multimode optical fiber.

[0021] In one possible implementation, the reference unit also includes a shutter assembly, which is used to control the reference beam to converge to the recording unit, or to block the reference beam from converging to the recording unit; when the shutter assembly blocks the reference beam from converging to the recording unit, the recording unit is used to obtain object light and determine the second transmission matrix based on the object light.

[0022] It should be understood that the reference beam is used for calibration of the device that emits the second laser, and no reference beam is required for calibration of the device that emits the first laser.

[0023] In one possible implementation, the control module is also communicatively connected to the modulation module; the control module is also used to determine a preset modulation strategy; wherein the preset modulation strategy includes a first modulation strategy and a second modulation strategy; the first modulation strategy is used to perform wavefront modulation on the first laser, and the second modulation strategy is used to perform wavefront modulation on the second laser.

[0024] It should be understood that the wavelengths of the first laser and the second laser are different. In order to allow both the first laser and the second laser to pass through the optical fiber module without interfering with each other, a preset modulation strategy is used to set different first modulation strategies and second modulation strategies corresponding to the first laser and the second laser. When the first laser is wavefront modulated, the second laser cannot be output to the optical fiber module. When the second laser is wavefront modulated, the first laser cannot be output to the optical fiber module.

[0025] In one possible implementation, the laser source module includes a first dimming unit, a second dimming unit, and a synthesis unit, wherein: the first dimming unit is used to control the intensity of the first laser; the second dimming unit is used to control the intensity of the second laser; the synthesis unit is used to synthesize the first laser and the second laser into a channel of light, wherein the first laser passes through the synthesis unit, and the second laser is reflected by the synthesis unit.

[0026] The first laser and the second laser are synthesized into a channel of light through a synthesis unit, which facilitates the modulation of the first laser or the second laser through a modulation module. Furthermore, the modulated control light and imaging light are integrated into a single optical fiber module, simplifying the part of the system that is inserted into living tissue and reducing damage to living tissue.

[0027] In a second aspect, a method for regulating living tissue cells is provided, which is applied to the regulation system for living tissue cells in the first aspect. The method includes: receiving a first laser and a second laser, wherein the first laser is used to regulate target cells based on optogenetics, and the second laser is used to image a target region, wherein the target region refers to the region to be regulated in the living tissue where the target cells are located; modulating the received first laser based on a preset modulation strategy to obtain modulated regulation light; modulating the received second laser based on a preset modulation strategy to obtain modulated imaging light, wherein the preset modulation strategy is a strategy for wavefront modulation of the first laser and the second laser; receiving a fluorescence signal in the target region, and determining a detection image corresponding to the target region based on the fluorescence signal, wherein the fluorescence signal is light emitted by the target region after being irradiated by the imaging light. Based on the detection image, determining a target regulation region where the target cells are located, wherein the target regulation region belongs to the region to be regulated; and regulating the target cells in the target regulation region based on the regulation light.

[0028] In a third aspect, a computer-readable storage medium is provided, wherein the computer-readable storage medium stores computer program code, and when the computer program code is executed, any one of the control methods for living tissue cells in the second aspect is executed.

[0029] In a fourth aspect, a computer program product is provided, the computer program product comprising: a computer program code, which, when executed, executes any one of the control methods for living tissue cells in the second aspect.

[0030] It can be understood that the beneficial effects of the second to fourth aspects mentioned above can be found in the relevant description of the first aspect mentioned above, and will not be repeated here.

[0031] Compared with the prior art, the embodiments of the present application have the following beneficial effects:

[0032] The embodiment of the present application provides a control system and method for living tissue cells, which includes a laser source module, a modulation module, an optical fiber module, an imaging module and a control module, wherein the laser source module is used to generate a first laser and a second laser, the first laser is used to control the target cells based on optogenetics, and the second laser is used to image the target area, which refers to the area to be controlled in the living tissue where the target cells are located; so that the system can realize imaging of living tissue and control of target cells in living tissue (such as stimulation, inhibition, intercellular communication, etc.), the modulation module is used to modulate the received first laser based on a preset modulation strategy, determine the control light, and modulate the received second laser based on the preset modulation strategy. , determine the imaging light, the control light is used to control the target cells, and the imaging light is used to image the target area; through the same modulation module, different lasers are modulated, and the output of the two beams of light without interfering with each other is achieved, thereby improving the flexible switching between imaging the target area and regulating the target cells, and realizing the coexistence of the two lights; further, the optical fiber module is used to output the control light or the imaging light. In order to achieve the positioning of the target cells, the imaging module is used to receive the fluorescence signal of the target area after being irradiated by the imaging light through the optical fiber module, and determine the detection image corresponding to the target area based on the fluorescence signal; the control module is used to determine the target regulation area where the target cell is located based on the detection image, so that in the target regulation area, the target cell can be precisely regulated by the control light. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the embodiments or descriptions of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0034] Figure 1 Schematic diagram of the structure of the control system for living tissue cells provided in an embodiment of the present application;

[0035] Figure 2 Schematic diagram of the structure of the control system for living tissue cells provided in an embodiment of the present application;

[0036] Figure 3 Schematic diagram of the structure of the control system for living tissue cells provided in an embodiment of the present application;

[0037] Figure 4 Schematic diagram of the structure of the control system for living tissue cells provided in an embodiment of the present application;

[0038] Figure 5Schematic diagram of the structure of the control system for living tissue cells provided in an embodiment of the present application;

[0039] Figure 6 This is a comparison diagram of the effects of wavefront shaping provided by the embodiments of the present application;

[0040] Figure 7 Schematic diagram of a control system for imaging living tissue cells provided in an embodiment of the present application;

[0041] Figure 8 Schematic diagram of a control system for living tissue cells provided in an embodiment of the present application;

[0042] Figure 9 Schematic diagram of the flow chart of the regulation method for living tissue cells provided in the embodiments of the present application. DETAILED DESCRIPTION

[0043] In the following description, specific details such as specific system structures and techniques are provided for purposes of illustration rather than limitation to facilitate a thorough understanding of the embodiments of the present application. However, it will be apparent to those skilled in the art that the present application may be implemented in other embodiments without these specific details. In other cases, detailed descriptions of well-known systems, devices, circuits, and methods are omitted to avoid obscuring the description of the present application with unnecessary detail.

[0044] It should be understood that when used in the present specification and the appended claims, the term "comprising" indicates the presence of described features, integers, steps, operations, elements and / or components, but does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or collections thereof.

[0045] Optogenetics is the process of expressing light-sensitive proteins in specific cells (such as neurons, cardiomyocytes, hepatocytes, immune cells, etc.), and then regulating the activities of these specific cells through light of different wavelengths.

[0046] Photosensitive proteins are a class of proteins that respond to light signals and produce physiological reactions. They can be divided into excitatory and inhibitory photosensitive proteins based on the electrophysiological functions they produce. For example, channelrhodopsin and halorhodopsin can be genetically introduced into specific cells in the brain or other tissues. Once expressed in these cells, the proteins can be activated or inhibited by light of specific wavelengths, thereby manipulating the activity of these specific cells.

[0047] In the biomedical field, light has a limited penetration depth into living tissue. Specifically, living tissues corresponding to specific cells scatter and absorb light, affecting its propagation and limiting its penetration depth. This scattering and absorption of light by living tissue depends on its thickness; deep within living tissue, light scatters very rapidly, reducing the in situ photon flux and leading to a loss of spatial information. Consequently, optogenetics cannot be performed spatially accurately and / or deeply within the tissue.

[0048] For example, the deep regions of the brain, a living organism, include the thalamus, hypothalamus, brainstem, and cerebellum. These regions are interconnected and work in tandem with other parts of the brain to maintain normal physiological functions and behavioral performance. Therefore, regulating these deep regions of living tissue is extremely important.

[0049] In this case, two-photon excitation is proposed. Two-photon excitation reaches an excited state by simultaneously absorbing the energy of two photons, generating an excitation event, thereby reducing the scattering and absorption of light in living tissues, and can achieve local activation or inhibition of photosensitive proteins in deeper areas of living tissues.

[0050] The probability of the above excitation events is proportional to the square of the light intensity. Therefore, two-photon excitation only occurs at the focal position of the laser beam, where the light intensity is the highest. Although two-photon excitation has good penetration ability into living tissue, in deeper living tissue, the signal will attenuate with increasing depth; resulting in limited regulation of specific cells in the area to be regulated in living tissue by two-photon excitation.

[0051] It should also be understood that although two-photon excitation reduces light scattering, high-power light may still cause a certain degree of photodamage to cells or tissues, and two-photon excitation requires high-intensity lasers and corresponding dedicated equipment. The operating performance requirements of such professional equipment are high, resulting in high difficulty in operating the entire system.

[0052] In order to solve the above problems, the embodiment of the present application provides a regulation system and method for living tissue cells, which includes a laser source module, a modulation module, an optical fiber module, an imaging module and a control module, wherein the laser source module is used to generate a first laser and a second laser, the first laser is used to regulate the target cells based on optogenetics, and the second laser is used to image the target area, which refers to the area to be regulated in the living tissue where the target cells are located; so that the system can realize imaging of living tissue and regulation of target cells in living tissue (such as stimulation, inhibition, intercellular communication, etc.), the modulation module is used to modulate the received first laser based on a preset modulation strategy, determine the regulation light, and modulate the received second laser based on the preset modulation strategy. Modulation is performed to determine the imaging light. The regulating light is used to regulate the target cells, and the imaging light is used to image the target area. Through the same modulation module, different lasers are modulated, and the output of the two beams of light without interfering with each other is achieved, thereby improving the flexible switching between imaging the target area and regulating the target cells, and realizing the coexistence of the two lights. Furthermore, the optical fiber module is used to output the regulating light or the imaging light. In order to achieve the positioning of the target cells, the imaging module is used to receive the fluorescence signal emitted by the target area after being irradiated by the imaging light through the optical fiber module, and determine the detection image corresponding to the target area based on the fluorescence signal. The control module is used to determine the target regulation area where the target cell is located based on the detection image, so that the target cell can be accurately regulated by the regulating light in the target regulation area.

[0053] The following combination Figures 1 to 8 The regulatory system for living tissue cells provided in the examples of the present application is described in detail.

[0054] Figure 1 Schematic diagram of the structure of the control system for living tissue cells provided in the embodiment of the present application. Figure 1 As shown, the regulation system 100 for living tissue cells includes a laser source module 110 , a modulation module 120 , an optical fiber module 130 , an imaging module 140 and a control module 150 .

[0055] The laser source module 110 is used to generate a first laser and a second laser, wherein the first laser is used to regulate target cells based on optogenetics, and the second laser is used to image the target area.

[0056] The first laser and the second laser are emitted by different lasers. It should be understood that the wavelengths of the first laser and the second laser are different. The wavelength of the first laser depends on the target cells to be modulated. The wavelength of the second laser is different from the wavelength of the first laser and can be used to image the target area.

[0057] Among them, the target area refers to the area to be regulated in the living tissue where the target cells are located; it should be understood that the area to be regulated in the living tissue can be located in the superficial area of ​​the living tissue or in the deep area of ​​the living tissue. Although the application scenarios in the embodiments of the present application are mainly aimed at imaging, target cell positioning and regulation in the deep area of ​​the living tissue, it is also applicable to imaging, target cell positioning and regulation in the superficial area of ​​the living tissue.

[0058] For example, in the brain, the area to be regulated can be a superficial area of ​​the brain or a deep area of ​​the brain.

[0059] The first laser and the second laser generated in the laser source module 110 are incident on the modulation module 120 to achieve modulation of the first laser and the second laser.

[0060] The modulation module 120 is configured to modulate the received first laser light based on a preset modulation strategy to determine the control light, and to modulate the received second laser light based on a preset modulation strategy to determine the imaging light.

[0061] The preset modulation strategy is a strategy for performing wavefront modulation on the first laser and the second laser. The wavefront modulation of the first laser and the second laser is achieved respectively through different strategies in the preset modulation strategy.

[0062] In some embodiments, the preset modulation strategy includes a first modulation strategy and a second modulation strategy; the first modulation strategy is used to perform wavefront modulation on the first laser, and the second modulation strategy is used to perform wavefront modulation on the second laser.

[0063] In this case, when the wavefront of the first laser is modulated based on the first modulation strategy, the second laser can also be modulated based on the first modulation strategy. Similarly, when the wavefront of the second laser is modulated based on the second modulation strategy, the first laser can also be modulated based on the second modulation strategy. However, the result of modulating the second laser based on the first modulation strategy and the result of modulating the first laser based on the second modulation strategy are not required by the system in the imaging or control process. Therefore, a screening unit can be set in the modulation module 120. Through the screening unit, the first laser and the second laser modulated based on the first modulation strategy are screened to determine the modulated first laser (i.e., the control light) as the output light of the modulation module 120 at this time. Alternatively, the first laser and the second laser modulated based on the second modulation strategy are screened to determine the modulated second laser (i.e., the imaging light) as the output light of the modulation module 120 at this time.

[0064] The output light of the modulation module 120 , including the control light and the imaging light, is output through the optical fiber module 130 . That is, the optical fiber module 130 is used to output the control light or the imaging light.

[0065] It should be understood that, for the modulated output light, the regulating light is the same as the first laser, and is used to regulate the target cells; and the imaging light is the same as the second laser, and is used to image the target area.

[0066] It should be understood that both the control light and the imaging light are output through the optical fiber module 130. The optical fiber in the optical fiber module is a multimode optical fiber. The multimode optical fiber can transmit multiple modes of light at the same time. In the embodiment of the present application, the multimode optical fiber is used to transmit the control light and the imaging light.

[0067] The core diameter of multimode optical fiber (usually 50-100 microns) can provide higher light flux and greater flexibility through multimode optical fiber. At the same time, the small core diameter of multimode optical fiber can reduce the damage to living tissue during the use of the system. This low-damage system can expand the application of the system in various implementation scenarios.

[0068] In the embodiment of the present application, the control light and the imaging light are integrated into a single optical fiber module 130 , and the living tissue is imaged and controlled by one optical fiber module, thereby simplifying the system design.

[0069] In some embodiments, the optical fiber module 130 includes a fiber collimator (FC) and a multimode fiber (MMF). The control light and the imaging light are focused into the multimode fiber through the fiber collimator and emitted from the output end of the multimode fiber, thereby injecting the control light and the imaging light into the target area.

[0070] In order to accurately direct the controlled light into the intended deep target in the living tissue, visualize the living tissue, and determine the insertion path of the optical fiber module, it is necessary to realize visualization of the living tissue through the imaging module 140 .

[0071] The imaging module 140 is used to receive a fluorescence signal in the target area through the optical fiber module, and determine a detection image corresponding to the target area based on the fluorescence signal, wherein the fluorescence signal is the fluorescence emitted by the target area after being irradiated by the imaging light.

[0072] It should be understood that the imaging light output through the output end of the optical fiber module 130 will converge to form a point. If the detection image corresponding to the target area is determined, it is necessary to change the output of the imaging light through the output end of the optical fiber module 130 so that the output convergence point can scan the entire field of view corresponding to the target area for synthesizing the detection image corresponding to the target area.

[0073] That is to say, the imaging light outputs a light field at the output end of the optical fiber module 130. By changing the preset modulation strategy in the modulation module, the light field entering the optical fiber module 130 can be controlled, so that the energy at the output end of the optical fiber module 130 is gathered to form points, and then the entire field of view corresponding to the target area is scanned. The fluorescence generated in the target area is received by the optical fiber module and transmitted back to the imaging module 140. According to the intensity of the feedback fluorescence signal corresponding to each scanning point, the detection image of the target area corresponding to the entire field of view is reconstructed.

[0074] In some embodiments, the reflected fluorescent signal is filtered and separated by a dichroic mirror and then collected by a photodetector in the imaging module 140. The photodetector calculates the intensity of the energy after collection and synthesizes a detection image corresponding to the target area.

[0075] Photodetectors are based on the photoelectric effect. When light strikes the photosensitive surface of a photodetector, it generates photoelectrons or excited electrons. These electrons then generate electrical signals within the photodetector. In other words, photodetectors are used to detect light signals and convert them into electrical signals. Photodetectors can be photomultiplier tubes (PMTs).

[0076] The control module 150 is in communication with the imaging module 140 . The control module is used to determine the target regulation region where the target cell is located based on the detection image, and the target regulation region belongs to the region to be regulated.

[0077] In some embodiments, the control module is also communicated with the modulation module, and the control module is also used to determine a preset modulation strategy. For example, the preset modulation strategy includes a first modulation strategy and a second modulation strategy; the first modulation strategy is used to perform wavefront modulation on the first laser, and the second modulation strategy is used to perform wavefront modulation on the second laser.

[0078] Figure 2 Schematic diagram of the structure of the control system for living tissue cells provided in the embodiment of the present application, such as Figure 2 As shown, the optical fiber module 130 includes an optical fiber collimator 131 and a multimode optical fiber 132 ; the imaging module 140 includes a photodetector 141 and a first dichroic mirror 142 .

[0079] It should be understood that the outside of the multimode optical fiber 132 may have a protective structure, such as a protective cover. In some embodiments, the protective structure on the outside of the multimode optical fiber 132 may be partitioned. For example, the diameter of the side of the multimode optical fiber close to the optical fiber collimator 131 is larger than the diameter of the side of the multimode optical fiber close to the living tissue, so that the optical fiber module 130 is easy to grasp during operation and reduces damage when entering the living tissue.

[0080] In some embodiments, the imaging module 140 further includes a filter. Interference light may exist in the fluorescence generated in the target area. The filter filters out light of other colors and retains only the fluorescence band, thereby improving the accuracy of the imaging module in reconstructing the detection image.

[0081] It should be understood that in the optical path of the entire system, in order to reduce hardware interference between various devices, a reflector can be provided to change the direction of the optical path. The change in the direction of the optical path does not affect the realization of the functions of each device. Therefore, in the embodiment of the present application, there is no limitation on the setting of the reflector. In actual applications, it can be set based on the actual scenario.

[0082] In some embodiments, the modulation module includes a dimming unit and a modulation unit; the dimming unit is used to expand or scale the process light passing through the modulation module, and the process light includes at least a first laser and a second laser; the modulation unit is used to adjust the phase and intensity of the process light based on a preset modulation strategy.

[0083] It should be understood that in the modulation module, the first and second lasers are adjusted by the dimming unit and the modulation unit, and the process light includes the forms of the first and second lasers at various stages in the modulation module. For example, light after wavefront adjustment by the modulation unit also qualifies as process light.

[0084] In some embodiments, the preset modulation strategy can achieve both wavefront shaping (adjustment of phase and intensity) and light field adjustment in only one way.

[0085] In some embodiments, the dimming unit includes a first lens assembly and a second lens assembly, and the modulation unit includes a holographic modulation assembly; the first lens assembly is used to expand the first laser and the second laser; the holographic modulation assembly is used to adjust the phase and intensity of the expanded first laser and the second laser based on a preset modulation strategy to obtain the first output light and the second output light; the second lens assembly is used to scale the first output light and the second output light to determine the controlled light and the imaging light.

[0086] The preset modulation strategy adjusts the expanded first laser and the second laser by using a preset hologram, and the holographic modulation component adjusts the wavefronts of the first laser and the second laser by displaying the preset hologram.

[0087] The above-mentioned combination of the first lens assembly, the holographic modulation assembly and the second lens assembly realizes the wavefront adjustment of the first laser and the second laser, wherein the first laser and the second laser pass through the first lens assembly, the holographic modulation assembly and the second lens assembly in the embodiment of the present application in sequence, and can determine the control light for controlling the target cells and the imaging light for imaging the target area, so that the control light and the imaging light can be output through the same optical fiber module, thereby enabling the system to image the target area, accurately locate the target cells, and control the target cells.

[0088] In some embodiments, the dimming unit also includes a screening component; the screening component is used to screen the first target order among each diffraction order corresponding to the first output light, and the first target order among each diffraction order corresponding to the second output light, the controlled light is the light whose diffraction order in the first output light is the first target order, and the imaging light is the light whose diffraction order in the second output light is the first target order.

[0089] It should be understood that the wavelengths of the first laser and the second laser are different. By adjusting the expanded output light fields of the first laser and the second laser based on the preset hologram, the determined first output light and the second output light are also different. By preset the hologram, the first output light and the second output light can both have target levels that can pass through the screening component, so that the determined control light and imaging light can be output through the same optical fiber module, simplifying the part of the system that is inserted into living tissue.

[0090] For example, both the first and second lens assemblies can be implemented using a 4F system, and the holographic control component can be implemented using a spatial light modulator (SLM), such as a high-speed digital micromirror device (DMD). The modulation module modulates the wavefront using a preset modulation strategy, and achieves high-speed, diffraction-limited focusing through optical fibers, enabling precise positioning of target cells.

[0091] Among them, the 4F system is composed of two lenses with a focal length of F; the structure of a high-speed digital micromirror device usually includes an array of many tiny mirrors, which can be independently controlled by electrostatic attraction or other means to achieve light modulation.

[0092] To display a preset hologram pattern on the DMD, the digital information of the preset hologram needs to be loaded into the DMD, and the display of the preset hologram is achieved by controlling the deflection of the micromirrors in the DMD.

[0093] In some embodiments, the control module is further in communication with the modulation module; the control module is further configured to determine a preset modulation strategy, that is, the control module determines a preset hologram pattern and controls the DMD to display the preset hologram pattern.

[0094] It should be understood that the wavelengths of the first laser and the second laser are different, so there are also differences in the wavefront modulation of the first laser and the second laser based on the preset modulation strategy. Therefore, the preset hologram used for the first laser modulation is different from the preset hologram used for the second laser modulation.

[0095] In some embodiments, the preset modulation strategy includes a first modulation strategy and a second modulation strategy; the first modulation strategy is used to perform wavefront modulation on the first laser, and the second modulation strategy is used to perform wavefront modulation on the second laser. The first modulation strategy includes a first preset hologram, and the second modulation strategy includes a second preset hologram. Both the first preset hologram and the second preset hologram are preset holograms.

[0096] In some embodiments, the screening component includes a pinhole element, and a pinhole element is arranged in the second lens component. The pinhole element is used to screen the first output light corresponding to the first target order among each diffraction order, and the second output light corresponding to the first target order among each diffraction order. It should be understood that the controlled light is the light whose diffraction order in the first output light is the first target order, and the imaging light is the light whose diffraction order in the second output light is the first target order.

[0097] Figure 3 Schematic diagram of the structure of the control system for living tissue cells provided in the embodiment of the present application, such as Figure 3 As shown, the modulation module 120 includes a first 4F system 121, a high-speed digital micromirror device DMD122, a second 4F system 123 and a pinhole element 124, wherein the pinhole element 124 is arranged in the second 4F system 123 to block the diffraction order of light that is not the first target order.

[0098] It should be understood that the first laser and the second laser emitted by the laser source module are emitted into the first 4F system in the modulation module 120 through the same optical path, and the first laser and the second laser are expanded by the first 4F system 121 to cover the entire DMD122. Then, the preset hologram pattern is displayed on the DMD122 to realize modulation (including phase, intensity, etc.) of the expanded first laser and the second laser, thereby realizing wavefront shaping.

[0099] The first laser and the second laser are modulated simultaneously by the same preset hologram, and the first laser and the second laser have different wavelengths and different diffraction angles. Therefore, after the first laser and the second laser pass through the DMD122, they output first output light and second output light respectively.

[0100] If the preset hologram at this time is used to control the first laser, the corresponding pinhole element can pass the light with the first target diffraction order in the first output light, while the light of each diffraction order in the second output light cannot pass through the pinhole element.

[0101] If the preset hologram at this time is used to control the second laser, the corresponding pinhole element can pass the light with the first target diffraction order in the second output light, while the light of each diffraction order in the first output light cannot pass through the pinhole element.

[0102] That is to say, through the pinhole element, it is possible to control whether the light output by the modulation module 120 is imaging light or control light. The control light is the light in the first output light whose diffraction order is the first target order, and the imaging light is the light in the second output light whose diffraction order is the first target order.

[0103] It should be understood that the refresh rate of DMD122 is very high, so the raster scanning and switching speed of the two laser light sources are very fast, so that the light output by the multimode optical fiber 132 of the optical fiber module 130 includes imaging light and control light, and because the switching through DMD122 is fast, the two do not interfere with each other and are output accurately.

[0104] The above-mentioned wavefront shaping achieved through DMD122 can enable high-speed, diffraction-limited focusing of light to achieve precise positioning. At the same time, it can also pre-compensate for the modal dispersion of light, overcome the limitations of low temporal and spatial resolution stimulus transmission, and measure the complex light transfer function in the multimode optical fiber 132. It can form an arbitrary diffraction-limited focus of light at the far end of the multimode optical fiber 132, and realize high-precision control of light (stimulation, inhibition, etc.). In other words, single-cell control can be realized, and grating scanning of imaging light can achieve high-resolution real-time imaging.

[0105] The imaging module 140 includes a photodetector 141 and a first dichroic mirror 142. The fluorescence emitted by the target area after being irradiated by the imaging light is received again by the multimode optical fiber 132 and transmitted back to the imaging module 140. The reversely transmitted light passes through the first dichroic mirror 142 to filter and separate the reflected fluorescence signal, which is then collected by the photodetector 141. After collection, the photodetector 141 calculates the intensity of the energy and synthesizes the detection image corresponding to the target area.

[0106] After the imaging light is output from the output end of the multimode optical fiber, it converges to a point in the field of view of the target area. The field of view of the entire target area is then scanned. Based on the intensity of the fluorescence signal fed back from each corresponding scan point, the fluorescence imaging of the entire field of view is reconstructed, that is, the detection image is determined.

[0107] The scanning of the field of view of the target area may be performed from left to right and from top to bottom.

[0108] If the detected image contains the target cell, the target cell can be located and the position corresponding to the target cell, that is, the target regulatory region, can be determined.

[0109] Through the imaging module, images of each area to be regulated in living tissue can be fed back, target cells can be accurately located, and real-time feedback on the regulation effect of target cells can be obtained.

[0110] In some embodiments, the laser source module includes a first light source unit, a second light source unit, and a beam splitting unit. The first light source unit is configured to emit a first laser beam, the second light source unit is configured to emit a second laser beam, and the beam splitting unit is configured to split the second laser beam into a second signal beam and a reference beam. The second signal beam is configured to image a target area, and the reference beam is configured to calibrate the second signal beam. The first laser unit and the second laser unit are both light sources that can emit fixed wavelengths. The beam splitting unit can be a component that splits light, such as a beam splitter.

[0111] It should be understood that the second signal beam is calibrated by the reference beam to generate imaging light, wherein the reference beam interferes with the imaging light passing through the optical fiber module, thereby accurately measuring the transmission matrix of the imaging light through the optical fiber module.

[0112] In some embodiments, the beam splitting unit is further configured to split the first laser into a first signal beam, which is used to regulate the target cells. The beam splitting unit may include two beam splitters, one for splitting the first laser and the other for splitting the second laser.

[0113] In some embodiments, the laser source module includes a first dimming unit, a second dimming unit, and a combining unit, wherein: the first dimming unit is used to control the intensity of the first laser; the second dimming unit is used to control the intensity of the second laser; and the combining unit is used to combine the first laser and the second laser into a single channel of light, wherein the first laser passes through the combining unit and the second laser is reflected by the combining unit. The first dimming unit and the second dimming unit can be a single element or a combination of multiple elements, for example, a half-wave plate, a variable attenuator, or a combination of a half-wave plate and a polarizer, or a half-wave plate and a variable attenuator. The combining unit can be implemented using a dichroic mirror, a lens, etc.

[0114] It should be understood that a dichroic mirror can separate light of different wavelengths. Similarly, a dichroic mirror can also be used to combine two lights of different wavelengths into one beam of light.

[0115] However, the light emitted by a multimode fiber spreads out, producing a wide illumination area or a random speckle pattern not far from the end of the fiber. Therefore, a calibration module is required to improve the accuracy of the light emitted by the multimode fiber.

[0116] In order to achieve calibration of the output light of the optical fiber module, the control system 100 also includes a calibration module 160, which includes a reference unit and a recording unit; the reference unit is used to converge the reference beam to the recording unit; the recording unit is used to obtain a combined beam, and determine a first transmission matrix based on the interference between different lights in the combined beam, wherein the combined beam includes a reference beam and object light, and the object light is the control light or the imaging light irradiated on the target area.

[0117] In some embodiments, the reference unit further includes a shutter assembly, which is used to control the reference beam to converge to the recording unit, or to block the reference beam from converging to the recording unit; when the shutter assembly blocks the reference beam from converging to the recording unit, the recording unit is used to obtain object light and determine a second transmission matrix based on the object light.

[0118] It should be understood that the first light source unit and the second light source unit can be calibrated separately by considering the reference beam of the second laser, and when calibrating the first light source unit, the object light is the regulating light irradiated on the target area, and when calibrating the second light source unit, the object light is the imaging light irradiated on the target area.

[0119] Figure 4 Schematic diagram of the structure of the control system for living tissue cells provided in the embodiment of the present application, such as Figure 4 As shown, the laser source module 110 includes a first light source 111 , a second light source 112 , a first half-wave plate 113 , a second half-wave plate 114 , a first polarization beam splitter 115 , a second polarization beam splitter 116 and a second dichroic mirror 118 .

[0120] Among them, the first light source 111 emits a first laser, the intensity of which is controlled by the first half-wave plate 113 and the first polarization beam splitter 115, and then is emitted into the second dichroic mirror 118. The second light source 112 emits a second laser, the intensity of which is controlled by the second half-wave plate 114 and the second polarization beam splitter 116, and then the second laser is split into a second signal beam and a reference beam by the second polarization beam splitter 116, and the second signal beam is emitted into the second dichroic mirror 118. The second dichroic mirror 118 then emits the first laser and the second signal light into the modulation module.

[0121] In some embodiments, the laser source module 110 further includes a beam blocker 117, which is disposed on the first polarization beam splitter 115. The beam blocker 117 separates the first signal beam in the first laser light through the first polarization beam splitter 115 and blocks the beam of the first laser light separated by the first polarization beam splitter 115. This improves the accuracy of the beam of the first laser light entering the modulation module.

[0122] At this time, the first light source 111 emits a first laser, which passes through the first half-wave plate 113 and the first polarization beam splitter 115 to control the intensity of the first laser. When passing through the first polarization beam splitter 115, a first signal beam can also be separated from the first laser, and the first signal beam is emitted into the second dichroic mirror 118. The second light source 112 emits a second laser, which passes through the second half-wave plate 114 and the second polarization beam splitter 116 to control the intensity of the second laser, and then passes through the second polarization beam splitter 116 to split the second laser into a second signal beam and a reference beam, and the second signal beam is emitted into the second dichroic mirror 118. The second dichroic mirror 118 injects the first signal beam and the second signal light into the modulation module.

[0123] For the description of the modulation module 120, the optical fiber module 130 and the imaging module 140, please refer to the aforementioned Figure 3 Corresponding description in the corresponding embodiment.

[0124] It should be understood that, for the preset hologram displayed by the DMD 122 in the modulation module 120 , the control module can control the DMD 122 to display, and based on the modulation of different lasers, different preset holograms can be displayed accordingly.

[0125] In some embodiments, the preset hologram is a Lee hologram, that is, through DMD122, the second 4F system 123 and the pinhole element 124, DMD122 outputs the first output light and the second output light through the preset hologram, and determines the output corresponding control light and imaging light through the second 4F system 123 and the pinhole element 124.

[0126] like Figure 4 As shown, the calibration module 160 includes a recording unit consisting of an objective lens 161 , a third polarization beam splitter 162 and a camera 163 , and a reference unit consisting of a third 4F system 164 , a shutter 165 and a third half-wave plate 166 .

[0127] The calibration process includes calibration of the first light source 111 and calibration of the second light source 112 .

[0128] The process of calibrating the first light source 111 includes: the first laser beam is split by the first polarization beam splitter 115 into a first signal beam, which is then irradiated onto the target area through the modulation module 120 and the optical fiber module 130. This object light is collected by the objective lens, recorded by the camera 163, and the first transmission matrix of the multimode optical fiber is calculated. It should be understood that during the calibration of the first light source 111, the shutter 165 is closed, and the camera 163 only records the object light.

[0129] The process of calibrating the second light source 112 includes: the reference beam separated by the second laser through the second polarization beam splitter 116 passes through the third half-wave plate 166, the shutter 165 and the third 4F system 164, and is merged with the object light passing through the objective lens at the third polarization beam splitter 162. Further, it is recorded by the camera 163 and the second transmission matrix of the multimode optical fiber is calculated. At this time, the object light is the second signal beam separated by the second laser through the second polarization beam splitter 116 and irradiated on the target area through the modulation module 120 and the optical fiber module 130.

[0130] The above calibration process is performed before the system performs imaging and regulation.

[0131] Figure 5 Schematic diagram of the structure of the control system for living tissue cells provided in the embodiment of the present application, such as Figure 5 As shown, a schematic diagram of a scenario in which a control system for living tissue cells is applied to mouse brain samples. In the optical path of the entire system, in order to reduce hardware interference between various devices, a reflector can be provided to change the direction of the optical path. The change in the direction of the optical path does not affect the realization of the functions of each device. Therefore, in the embodiment of the present application, there is no limitation on the setting of the reflector. In actual applications, it can be set based on the actual scenario.

[0132] In this scenario, the system can refer to the above Figure 4 Corresponding description in the corresponding embodiment.

[0133] Figure 6 This is a comparison chart of the effects of wavefront shaping provided by the embodiment of the present application, applied to Figure 5 The regulatory system for living tissue cells shown, such as Figure 6 As shown, Figure 6 (a) shows the effect without wavefront shaping. Figure 6 (b) shows the effect of real-time wavefront shaping. By measuring and pre-compensating the complex optical transfer function of the multimode optical fiber through wavefront shaping, an arbitrary light diffraction-limited focus can be formed at the far end of the multimode optical fiber, which is used for high-precision light control and high-resolution real-time imaging of grating scanning.

[0134] Figure 7 Schematic diagram of a control system for imaging living tissue cells provided in an embodiment of the present application, such as Figure 7 As shown, Figure 7 (a) is the fluorescent bead display. Figure 7 (b) is displayed in the target area. Figure 7 (c) Fluorescence imaging of the target area with target cells.

[0135] It should be understood that the imaging process is performed after verification.

[0136] Figure 8 Schematic diagram of the control system for living tissue cells provided in the embodiment of the present application. Figure 8 As shown, Figure 8 (a) is calcium imaging under blue light illumination, which serves as the background F b ; Figure 8 (b) shows the neuron in quiet conditions without light stimulation, recorded as the difference between the image and the background ΔF s / F b . Figure 8 In (c), there is no wavefront shaping, and the light output from the multimode fiber is a random speckle pattern, which is recorded as the difference between the image and the background ΔF0 / F b , confirming that all neurons in the visual field appeared activated. Figure 8 Based on the obtained projection matrix, phase masks for generating one and two focal regions can be calculated and uploaded to the DMD. The corresponding differences in the images relative to the background image are shown in (d) and (e), respectively, confirming the selective activation of neurons for precise neuronal activation through multimode optical fibers. Figure 8 (f) shows the statistical data of repeated stimulation of target cells in (d).

[0137] After imaging to determine the target regulatory region where the target cells are located, the target cells can be stimulated and regulated.

[0138] It should be understood that the above is an example of an application scenario and does not limit the application scenario of this application.

[0139] It should be understood that the above examples are intended to help those skilled in the art understand the embodiments of the present application, and are not intended to limit the embodiments of the present application to the specific numerical values ​​or specific scenarios illustrated. Those skilled in the art can obviously make various equivalent modifications or variations based on the above examples, and such modifications or variations also fall within the scope of the embodiments of the present application.

[0140] Combined with the above Figures 1 to 8 , describes in detail the control system for living tissue cells of the embodiment of the present application, and will be combined with Figure 9 , describing in detail the control method for living tissue cells of the present application. It should be understood that the control method for living tissue cells in the embodiments of the present application is applied to the control system for living tissue cells, that is, the implementation process of each of the following method embodiments can refer to the corresponding description in the aforementioned system embodiment.

[0141] Figure 9 Schematic diagram of the process for regulating living tissue cells provided in the embodiment of the present application. Figure 9 As shown, the method includes:

[0142] S210 , receiving a first laser and a second laser.

[0143] The first laser is used to regulate the target cells based on optogenetics, and the second laser is used to image the target area, which refers to the area to be regulated in the living tissue where the target cells are located.

[0144] S220 , modulating the received first laser based on a preset modulation strategy to obtain modulated control light, and modulating the received second laser based on a preset modulation strategy to obtain modulated imaging light.

[0145] Wherein, the preset modulation strategy is a strategy for performing wavefront modulation on the first laser and the second laser;

[0146] S230 , receiving a fluorescent signal in the target area, and determining a detection image corresponding to the target area based on the fluorescent signal.

[0147] Among them, the fluorescence signal is the light emitted by the target area after being illuminated by the imaging light.

[0148] S240. Determine the target regulatory region where the target cell is located based on the detection image.

[0149] Among them, the target regulation area belongs to the area to be regulated;

[0150] S250. In the target regulation area, the target cells are regulated based on the regulation light.

[0151] Referring to the corresponding description in the aforementioned system embodiment, the method for controlling living tissue cells includes calibration, imaging, and control. When a multimode optical fiber is inserted, this method provides a view of the target area, allowing for more accurate placement of the stimulus within the target area where the target cells reside, achieving control. The control process can also be displayed in real time. This real-time feedback enables reliable and high-precision targeting of deep regions of living cells (e.g., deep brain regions).

[0152] The present application also provides a computer-readable storage medium having a computer program stored thereon. When executed by a computer, the computer program implements the method for regulating living tissue cells according to any of the method embodiments of the present application. The computer program can be a high-level language program or an executable object program.

[0153] An embodiment of the present application further provides a computer program product, which, when executed on a mobile terminal, enables the mobile terminal to implement the method for regulating living tissue cells of any method embodiment of the present application.

[0154] In the above embodiments, the description of each embodiment has its own focus. For parts that are not described or recorded in detail in a certain embodiment, reference can be made to the relevant description of other embodiments.

[0155] Those skilled in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of this application.

[0156] Those skilled in the art will clearly understand that, for the convenience and brevity of description, the specific working processes of the systems, devices and units described above can refer to the corresponding processes in the aforementioned method embodiments and will not be repeated here.

[0157] In the several embodiments provided in this application, it should be understood that the disclosed devices and methods can be implemented in other ways. For example, the device embodiments described above are merely illustrative; for example, the division of units is merely a logical function division, and there may be other division methods in actual implementation; for example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms.

[0158] Units described as separate components may or may not be physically separate, and components shown as units may or may not be physical units, that is, they may be located in one place or distributed across multiple network units. Some or all of these units may be selected to achieve the purpose of this embodiment according to actual needs.

[0159] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit.

[0160] The above are only specific embodiments of the present application, but the scope of protection of this application is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in this application should be included in the scope of protection of this application. Therefore, the scope of protection of this application should be based on the scope of protection of the claims.

Claims

1. A control system for living tissue cells, characterized in that: include: a laser source module, the laser source module being configured to generate a first laser and a second laser, wherein the first laser is configured to regulate target cells based on optogenetics, and the second laser is configured to image a target region, wherein the target region is a region to be regulated in a living tissue where the target cells are located; a modulation module, the modulation module being configured to modulate the received first laser light based on a preset modulation strategy to determine a controlled light, and to modulate the received second laser light based on a preset modulation strategy to determine an imaging light, wherein the preset modulation strategy is a strategy for performing wavefront modulation on the first laser light and the second laser light; an optical fiber module, the optical fiber module being used to output the regulating light or the imaging light, the regulating light being used to regulate the target cells; an imaging module, configured to receive a fluorescence signal in the target area through the optical fiber module, and determine a detection image corresponding to the target area based on the fluorescence signal, wherein the fluorescence signal is light emitted by the target area after being illuminated by the imaging light; A control module is communicatively connected to the imaging module, and is used to determine a target regulation region where the target cell is located based on the detection image, where the target regulation region belongs to the region to be regulated.

2. The control system for living tissue cells according to claim 1, characterized in that: The modulation module includes a dimming unit and a modulation unit; The dimming unit is used to expand or scale the process light passing through the modulation module, and the process light at least includes the first laser and the second laser; The modulation unit is used to adjust the phase and intensity of the process light based on a preset modulation strategy.

3. The control system for living tissue cells according to claim 2, characterized in that: The dimming unit includes a first lens assembly and a second lens assembly, and the modulation unit includes a holographic modulation assembly; The first lens assembly is used to expand the first laser and the second laser; The holographic modulation component is used to adjust the phase and intensity of the expanded first laser and the second laser based on a preset modulation strategy to obtain a first output light and a second output light; The second lens assembly is used to scale the first output light and the second output light to determine the controlled light and the imaging light.

4. The control system for living tissue cells according to claim 3, characterized in that: The dimming unit further includes a screening component; The screening component is used to screen the first target order among each diffraction order corresponding to the first output light, and the first target order among each diffraction order corresponding to the second output light. The controlled light is the light whose diffraction order in the first output light is the first target order, and the imaging light is the light whose diffraction order in the second output light is the first target order.

5. The control system for living tissue cells according to claim 1, characterized in that: The laser source module includes a beam splitting unit, The beam splitting unit is used to split the second laser into a second signal beam and a reference beam, the second signal beam is used to image the target area, and the reference beam is used to calibrate the second signal beam.

6. The control system for living tissue cells according to claim 5, characterized in that: The system further comprises a calibration module, wherein the calibration module comprises a reference unit and a recording unit; The reference unit is used to converge the reference beam to the recording unit; The recording unit is used to obtain a combined light beam and determine a first transmission matrix based on interference between different lights in the combined light beam, wherein the combined light beam includes the reference light beam and object light, and the object light is the controlled light or the imaging light irradiated on the target area.

7. The control system for living tissue cells according to claim 6, characterized in that: The reference unit further includes a shutter assembly, and the shutter assembly is used to control the reference beam to converge to the recording unit, or to block the reference beam from converging to the recording unit; When the shutter assembly blocks the reference beam from converging to the recording unit, the recording unit is used to obtain the object light and determine a second transmission matrix based on the object light.

8. The control system for living tissue cells according to claim 1, characterized in that: The control module is also in communication with the modulation module; The control module is further configured to determine the preset modulation strategy; The preset modulation strategy includes a first modulation strategy and a second modulation strategy; the first modulation strategy is used to perform wavefront modulation on the first laser, and the second modulation strategy is used to perform wavefront modulation on the second laser.

9. The control system for living tissue cells according to claim 1, characterized in that: The laser source module includes a first dimming unit, a second dimming unit and a synthesis unit, wherein: The first dimming unit is used to control the intensity of the first laser; The second dimming unit is used to control the intensity of the second laser; The synthesis unit is used to synthesize the first laser light and the second laser light into light of one channel, wherein the first laser light passes through the synthesis unit, and the second laser light is reflected by the synthesis unit.

10. A method for regulating living tissue cells, characterized in that: Applied to the regulatory system for living tissue cells, the method comprises: receiving a first laser and a second laser, wherein the first laser is used to regulate target cells based on optogenetics, and the second laser is used to image a target area, where the target area refers to an area to be regulated in a living tissue where the target cells are located; Based on a preset modulation strategy, modulating the received first laser to obtain modulated control light; based on a preset modulation strategy, modulating the received second laser to obtain modulated imaging light, wherein the preset modulation strategy is a strategy for wavefront modulation of the first laser and the second laser; receiving a fluorescence signal in the target area, and determining a detection image corresponding to the target area based on the fluorescence signal, wherein the fluorescence signal is light emitted by the target area after being illuminated by the imaging light; Determining, based on the detection image, a target regulatory region where the target cell is located, wherein the target regulatory region belongs to the region to be regulated; In the target regulation region, the target cells are regulated based on the regulation light.

Images