Preparation method of animal feed based on Chinese prickly ash seed meal

Abstract

The invention discloses a preparation method of animal feed based on Chinese prickly ash seed meal, and belongs to the technical field of breeding. The animal feed based on Chinese prickly ash seed meal comprises the following raw materials in parts by mass: 15-20 parts of Chinese prickly ash seed meal, 30-40 parts of corn flour, 10-15 parts of soybean meal, 10-12 parts of wheat flour, 10-12 parts of straw, 10-12 parts of mixed powder of orange peel and bagasse, 3-4 parts of jerusalem artichoke powder, 1-1.5 parts of vanilla powder, 5-8 parts of fermented soybean meal and 1-1.5 parts of compound microorganisms. The animal feed disclosed by the invention can improve the intestinal health of animals, promote the absorption and utilization of nutrient substances, promote the growth of the animals and improve the meat quality of the animals.

Description

Technical Field

[0001] The present invention relates to the technical field of breeding, and in particular to a method for preparing animal feed based on Zanthoxylum bungeanum seed meal. Background Art

[0002] In recent years, with the rapid development of animal husbandry and people's increasing concern for food safety and health, the requirements for the quality and functionality of feed ingredients have become increasingly higher. Traditional feed ingredients can no longer meet the needs of modern animal husbandry. Therefore, the search and development of new functional feed additives has become a hot topic in current research.

[0003] Sichuan pepper seed meal, a byproduct of oil extraction from Sichuan pepper seeds, is rich in dietary fiber and a natural, environmentally friendly feed additive with multiple physiological functions, boasting significant health benefits and economic value. However, Sichuan pepper seed meal has drawbacks such as high levels of crude fiber and anti-nutritional factors (such as tannins and saponins), low digestibility, and poor palatability (including a spicy flavor), which reduces its feeding value. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for preparing animal feed based on Zanthoxylum bungeanum seed meal, so as to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] One of the technical solutions of the present invention is an animal feed based on pepper seed meal, comprising the following raw materials in parts by mass: 15 to 20 parts of pepper seed meal, 30 to 40 parts of corn flour, 10 to 15 parts of soybean meal, 10 to 12 parts of wheat flour, 10 to 12 parts of straw, 10 to 12 parts of a mixture of orange peel and sugarcane bagasse, 3 to 4 parts of Jerusalem artichoke powder, 1 to 1.5 parts of vanilla powder, 5 to 8 parts of fermented soybean meal and 1 to 1.5 parts of composite microorganisms.

[0007] Furthermore, the method for preparing the fermented soybean meal comprises the following steps:

[0008] The soybean meal is crushed and then evenly mixed with water and composite probiotics, and then sealed and fermented to obtain the fermented soybean meal.

[0009] Furthermore, the mass ratio of the soybean meal, water and composite probiotics is (30-40): (10-15): (1-1.5);

[0010] The composite probiotic comprises the following components in parts by weight: 3-4 parts of Bacillus subtilis liquid, 2-3 parts of Lactobacillus plantarum liquid, and 5-8 parts of Bifidobacterium longum liquid;

[0011] The temperature of the sealed fermentation is 30-35° C., and the time is 8-10 days.

[0012] Fermenting soybean meal with Bacillus subtilis, Lactobacillus plantarum and Bifidobacterium longum can increase the content of flavor substances, which can work synergistically with vanilla powder to improve the palatability of feed.

[0013] Furthermore, the composite microorganism comprises the following components in parts by mass: 3 to 5 parts of Bacillus licheniformis solution, 4 to 6 parts of Bacillus megaterium solution, 2 to 3 parts of Lactobacillus acidophilus solution and 4 to 6 parts of Candida krusei solution.

[0014] The synergistic effect of Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus and Candida krusei can significantly improve the intestinal health of animals, regulate the excellent intestinal flora, and promote the absorption and utilization of nutrients by animals.

[0015] Soybean flour and Jerusalem artichoke flour are rich in oligosaccharides, which can promote the growth of beneficial bacteria, inhibit harmful bacteria, and thus improve the intestinal health of animals and promote the absorption and utilization of nutrients by animals.

[0016] Furthermore, the method for preparing the orange peel and bagasse mixed powder comprises the following steps:

[0017] Fresh orange peel, sugarcane bagasse and water are mixed in a mass ratio of 1:10:5, steamed, dried and crushed to obtain the orange peel and sugarcane bagasse mixed powder.

[0018] Furthermore, the steaming temperature is 95-105° C. and the time is 10-15 min;

[0019] The drying temperature is 70-80°C.

[0020] Mixing orange peel and sugarcane bagasse and then steaming and drying them can remove the bitter substances in the orange peel and improve the palatability of the feed; at the same time, the citric acid contained in the orange peel can lower the pH value of the gastrointestinal tract, inhibit harmful bacteria, enhance the activity of digestive enzymes, and improve the absorption and utilization rate of feed.

[0021] The second technical solution of the present invention is a method for preparing the above-mentioned animal feed, comprising the following steps:

[0022] (1) steam explosion treatment is performed on the Zanthoxylum bungeanum seed meal to obtain pretreated Zanthoxylum bungeanum seed meal;

[0023] (2) The pretreated pepper seed meal, straw and fermented soybean meal are mixed evenly and then crushed, mixed evenly with the remaining raw materials, and granulated at low temperature to obtain the animal feed.

[0024] Furthermore, the steam pressure of the steam explosion treatment is 1.3 MPa, the water content of the material is 35%, and the pressure maintenance time is 140 s.

[0025] Furthermore, step (1) specifically comprises: sequentially performing steam explosion treatment and fermentation treatment on the Zanthoxylum bungeanum seed meal to obtain pretreated Zanthoxylum bungeanum seed meal;

[0026] The fermentation temperature is 30-40°C and the time is 12-24 hours;

[0027] The fermentation bacteria used in the fermentation process is Rhodotorula glutinosus.

[0028] Red yeast can effectively decompose the spicy substances in pepper seed meal and improve the palatability of feed containing pepper seed meal.

[0029] Steam explosion and fermentation of pepper seed meal can significantly destroy the cell wall, increase the soluble dietary fiber content, and reduce the crude fiber and anti-nutritional factor content.

[0030] The third technical solution of the present invention: an application of the above-mentioned animal feed as livestock and poultry breeding feed.

[0031] Furthermore, the livestock and poultry farming includes broiler farming.

[0032] The present invention discloses the following technical effects:

[0033] (1) The animal feed of the present invention can improve the intestinal health of animals, promote the absorption and utilization of nutrients, promote animal growth, and improve the meat quality and quality of animals.

[0034] (2) The animal feed of the present invention effectively utilizes the byproduct of Zanthoxylum bungeanum seed oil extraction, which is the Zanthoxylum bungeanum seed meal, thereby improving the feeding value of the Zanthoxylum bungeanum seed meal. DETAILED DESCRIPTION

[0035] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0036] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between any intermediate value within a stated value or stated range and any other stated value or intermediate value within the stated range is also encompassed by the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0037] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0038] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be exemplary only.

[0039] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0040] It should be pointed out that the matters not described in detail in the present invention are conventional operating means in this field and are not the focus of the present invention.

[0041] Sichuan peppercorn seeds are the primary byproduct of Sichuan peppercorn peel production. Theoretically, their yield is about 20% higher than the peel itself. These seeds are black, shiny, rich in oil and protein, and account for 60-70% of the total Sichuan peppercorn production. However, the majority of these seeds have long been underutilized. In production areas, large quantities are burned as fuel, scattered on fields as fertilizer, or even discarded as waste. As a byproduct, accounting for more than half of total Sichuan peppercorn production, these seeds are used solely for oil extraction. The resulting oil is dark, high in acidity, easily crystallizes, has a pungent odor, and is therefore inedible.

[0042] (1) Nutritional properties of Sichuan pepper seeds

[0043] Yang Lingye et al. (2007) reported that Hancheng Dahongpao pepper seeds contain 13.66% moisture and 19.79% protein, with the kernel containing as much as 41.17% protein. Crude fiber can reach 24.73%, with the shell containing 39.25% crude fiber. Oil content reaches 32.44%, with the kernel containing 46.55% oil, and the kernel oil is of high quality. Analysis by Li Guihua, Zhuang Shihong et al. shows that in addition to 27.1% oil, pepper seeds also contain approximately 18.7% crude protein, 30.23% crude fiber (in the shell), 15-20% wax (in the shell), and approximately 10% water, ash, and volatiles.

[0044] (2) Protein in Sichuan pepper seeds

[0045] Yang Lingye et al. (2008) conducted an Osborne protein classification of Dahongpao Zanthoxylum bungeanum kernel proteins, isolating them into 16.38% albumin, 41.09% globulin, 2.53% alcohol-soluble protein, and 33.29% glutenin. These proteins were then subjected to SDS-PAGE electrophoresis to determine subunit size. The results showed that the protein fractions contained a significant amount of salt-soluble globulin and alkali-soluble glutenin, making them easily extractable using salting or dilute alkali. Furthermore, the relative molecular weights of the various protein components in the Zanthoxylum bungeanum kernels were relatively low, facilitating digestion and absorption. Compared to soybeans, Zanthoxylum bungeanum kernels contain lower levels of lysine and histidine, but the content of other essential amino acids is similar to or higher than that of soybeans, making it a relatively complete protein. This can be used to produce products similar to soy protein isolates. Zanthoxylum bungeanum kernel meal also contains a high protein content, with laboratory measurements showing approximately 19%, making it a good source of feed protein.

[0046] All “parts” described in the following examples are “parts by mass”.

[0047] 1. The Zanthoxylum bungeanum seed meal used in the specific embodiment of the present invention was purchased from Shandong Jiuxin Green Zanthoxylum bungeanum Bioengineering Co., Ltd.

[0048] 2. The Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Candida krusei, Bacillus subtilis, Lactobacillus plantarum, Bifidobacterium longum, Rhodotorula glutinosus, and Saccharomyces cerevisiae used in the specific embodiments of the present invention were purchased from the China Industrial Microorganism Culture Collection Center;

[0049] Among them, the product number of Bacillus licheniformis is CICC 10103; the product number of Bacillus megaterium is CICC 23076; the product number of Lactobacillus acidophilus is CICC 6082; the product number of Candida krusei is CICC 1673; the product number of Bacillus subtilis is CICC 10732; the product number of Lactobacillus plantarum is CICC 24936; the product number of Bifidobacterium longum is CICC 6188; the product number of Rhodotorula glutinosus is CICC 32140; the product number of Saccharomyces cerevisiae is CICC 32883;

[0050] The preparation methods of Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Candida krusei, Bacillus subtilis, Lactobacillus plantarum, Bifidobacterium longum, Rhodotorula glutinosus, and Saccharomyces cerevisiae used in the specific embodiments of the present invention are as follows:

[0051] Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Candida krusei, Bacillus subtilis, Lactobacillus plantarum, Bifidobacterium longum, Rhodotorula glutinosus and Saccharomyces cerevisiae were inoculated into liquid culture medium for activation culture and fermentation to obtain bacterial counts of 1.0×10 8cfu / mL of Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Candida krusei, Bacillus subtilis, Lactobacillus plantarum, Bifidobacterium longum, Rhodotorula glutinosus, and Saccharomyces cerevisiae;

[0052] Liquid culture medium composition: peptone 5.0 g / L, beef extract 3.0 g / L, and sodium chloride 5.0 g / L, pH 7.0; moist heat sterilization for 20 min.

[0053] 3. The corn flour, soybean flour, wheat flour, Jerusalem artichoke flour and vanilla flour used in the specific embodiment of the present invention all have a particle size of 300 mesh and are all powdered after being dried (at a temperature of 40° C.) and then crushed.

[0054] Example 1

[0055] A method for preparing animal feed based on pepper seed meal:

[0056] (1) Animal feed, consisting of the following raw materials in parts by weight: 18 parts of Sichuan pepper seed meal, 40 parts of corn flour, 12 parts of soybean flour, 10 parts of wheat flour, 12 parts of straw, 12 parts of mixed powder of orange peel and sugarcane bagasse, 3.5 parts of Jerusalem artichoke powder, 1.5 parts of vanilla powder, 6 parts of fermented soybean meal and 1.2 parts of composite microorganisms.

[0057] The composite microorganism is composed of the following components in parts by mass: 4 parts of Bacillus licheniformis liquid, 5 parts of Bacillus megaterium liquid, 2 parts of Lactobacillus acidophilus liquid and 6 parts of Candida krusei liquid.

[0058] (2) Preparation method of pepper seed meal

[0059] The water content of the pepper seed meal was adjusted to 35%, and then the mixture was subjected to steam explosion treatment (steam pressure of 1.3 MPa, pressure maintenance time of 140 s), and dried (temperature of 50° C.) to obtain pretreated pepper seed meal.

[0060] (3) Preparation method of orange peel and bagasse mixed powder

[0061] Fresh orange peel, sugarcane bagasse and water are mixed in a mass ratio of 1:10:5, placed in a container and steamed (steaming temperature is 105°C, time is 12 minutes), then dried (drying temperature is 80°C), and crushed to 300 mesh to obtain orange peel and sugarcane bagasse mixed powder.

[0062] (4) Preparation method of fermented soybean meal

[0063] The soybean meal was crushed into 40 meshes to obtain soybean meal powder, and the soybean meal powder was evenly mixed with water and compound probiotics (the mass ratio of soybean meal, water and compound probiotics was 35:15:1.2), and sealed and fermented (the sealed fermentation temperature was 32°C and the time was 10 days) to obtain fermented soybean meal.

[0064] Among them, the composite probiotics are composed of the following components in parts by mass: 3.5 parts of Bacillus subtilis liquid, 2 parts of Lactobacillus plantarum liquid and 6 parts of Bifidobacterium longum liquid.

[0065] (5) The pretreated pepper seed meal, straw and fermented soybean meal were mixed evenly and then crushed to 300 mesh, and then mixed evenly with the remaining raw materials, and granulated at low temperature (temperature of 40°C, particle size of 1 mm) to obtain animal feed.

[0066] Example 2

[0067] A method for preparing animal feed based on pepper seed meal:

[0068] (1) Animal feed, consisting of the following raw materials in parts by weight: 20 parts of Sichuan pepper seed meal, 30 parts of corn flour, 15 parts of soybean flour, 12 parts of wheat flour, 11 parts of straw, 10 parts of mixed powder of orange peel and sugarcane bagasse, 3 parts of Jerusalem artichoke powder, 1 part of vanilla powder, 5 parts of fermented soybean meal and 1.5 parts of composite microorganisms.

[0069] The composite microorganism is composed of the following components in parts by mass: 5 parts of Bacillus licheniformis solution, 6 parts of Bacillus megaterium solution, 2 parts of Lactobacillus acidophilus solution and 4 parts of Candida krusei solution.

[0070] (2) Preparation method of pepper seed meal

[0071] The water content of the pepper seed meal was adjusted to 35%, and then the mixture was subjected to steam explosion treatment (steam pressure of 1.3 MPa, pressure maintenance time of 140 s), and dried (temperature of 50° C.) to obtain pretreated pepper seed meal.

[0072] (3) Preparation method of orange peel and bagasse mixed powder

[0073] Fresh orange peel, sugarcane bagasse and water are mixed in a mass ratio of 1:10:5, placed in a container and steamed (steaming temperature is 95°C, time is 10 minutes), then dried (drying temperature is 80°C), and crushed to 100 mesh to obtain orange peel and sugarcane bagasse mixed powder.

[0074] (4) Preparation method of fermented soybean meal

[0075] The soybean meal was crushed into 40 meshes to obtain soybean meal powder, and the soybean meal powder was evenly mixed with water and compound probiotics (the mass ratio of soybean meal, water and compound probiotics was 30:12:1.5), and sealed and fermented (the sealed fermentation temperature was 30°C and the time was 10 days) to obtain fermented soybean meal.

[0076] Among them, the compound probiotics are composed of the following components in parts by mass: 4 parts of Bacillus subtilis liquid, 3 parts of Lactobacillus plantarum liquid and 5 parts of Bifidobacterium longum liquid.

[0077] (5) The pretreated pepper seed meal, straw and fermented soybean meal were mixed evenly and then crushed to 300 mesh, and then mixed evenly with the remaining raw materials, and granulated at low temperature (temperature of 40°C, particle size of 1 mm) to obtain animal feed.

[0078] Example 3

[0079] A method for preparing animal feed based on pepper seed meal:

[0080] (1) Animal feed, consisting of the following raw materials in parts by weight: 15 parts of Sichuan pepper seed meal, 35 parts of corn flour, 10 parts of soybean flour, 10 parts of wheat flour, 12 parts of straw, 12 parts of mixed powder of orange peel and sugarcane bagasse, 4 parts of Jerusalem artichoke powder, 1 part of vanilla powder, 8 parts of fermented soybean meal and 1 part of composite microorganism.

[0081] The composite microorganism is composed of the following components in parts by mass: 3 parts of Bacillus licheniformis solution, 5 parts of Bacillus megaterium solution, 3 parts of Lactobacillus acidophilus solution and 6 parts of Candida krusei solution.

[0082] (2) Preparation method of pepper seed meal

[0083] The water content of the pepper seed meal was adjusted to 35%, and then the mixture was subjected to steam explosion treatment (steam pressure of 1.3 MPa, pressure maintenance time of 140 s), and dried (temperature of 50° C.) to obtain pretreated pepper seed meal.

[0084] (3) Preparation method of orange peel and bagasse mixed powder

[0085] Fresh orange peel, sugarcane bagasse and water are mixed in a mass ratio of 1:10:5, placed in a container and steamed (steaming temperature is 105°C, time is 15 minutes), then dried (drying temperature is 80°C), and crushed to 100 mesh to obtain orange peel and sugarcane bagasse mixed powder.

[0086] (4) Preparation method of fermented soybean meal

[0087] The soybean meal was crushed into 40 meshes to obtain soybean meal powder, and the soybean meal powder was evenly mixed with water and compound probiotics (the mass ratio of soybean meal, water and compound probiotics was 35:10:1), and sealed and fermented (the sealed fermentation temperature was 35°C and the time was 8 days) to obtain fermented soybean meal.

[0088] Among them, the composite probiotics are composed of the following components in parts by mass: 3 parts of Bacillus subtilis liquid, 2 parts of Lactobacillus plantarum liquid and 8 parts of Bifidobacterium longum liquid.

[0089] (5) The pretreated pepper seed meal, straw and fermented soybean meal were mixed evenly and then crushed to 300 mesh, and then mixed evenly with the remaining raw materials, and granulated at low temperature (temperature of 40°C, particle size of 1 mm) to obtain animal feed.

[0090] Example 4

[0091] Same as Example 1, except that step (2) is specifically as follows:

[0092] The water content of the pepper seed meal was adjusted to 35%, and then steam explosion treatment was performed (steam pressure of 1.3 MPa, pressure maintenance time of 140 s), and drying (temperature of 50° C.) was performed to obtain an intermediate material;

[0093] The intermediate material and the red yeast rice liquid were mixed at a mass ratio of 100:1, and the water content was adjusted to 50%. The mixture was sealed and fermented (temperature of 35° C., time of 20 h) to obtain pretreated Zanthoxylum bungeanum seed meal.

[0094] Comparative Example 1

[0095] Same as Example 1, except that soybean flour is replaced with corn flour of equal mass.

[0096] Comparative Example 2

[0097] The same as Example 1, except that the orange peel and bagasse mixed powder is replaced with citric acid in equal parts by mass.

[0098] Comparative Example 3

[0099] Same as Example 1, except that the mixed powder of orange peel and bagasse is replaced with orange peel powder in equal parts by weight;

[0100] The preparation method of orange peel powder is as follows: fresh orange peel and water are mixed in a mass ratio of 11:5, placed in a container for steaming (steaming temperature is 105°C, time is 12 minutes), then dried (drying temperature is 80°C), and crushed to 300 mesh to obtain orange peel powder.

[0101] Comparative Example 4

[0102] The same as Example 1, except that Jerusalem artichoke powder is replaced with asparagus powder (powder made by drying asparagus (temperature is 40° C.) and crushing it) in equal parts by mass.

[0103] Comparative Example 5

[0104] Same as Example 1, except that the animal feed does not contain fermented soybean meal.

[0105] Comparative Example 6

[0106] The same as Example 1, except that the composite probiotics used in preparing the fermented soybean meal consisted of the following components in parts by mass: 3.5 parts of Bacillus subtilis liquid, 2 parts of Bacillus megaterium liquid, and 6 parts of Bacillus licheniformis liquid.

[0107] Comparative Example 7

[0108] The same as Example 1, except that the Candida krusei bacterial solution in the composite microorganism is replaced by an equal mass portion of Bacillus subtilis bacterial solution, and the Lactobacillus acidophilus bacterial solution is replaced by an equal mass portion of Lactobacillus plantarum bacterial solution.

[0109] Comparative Example 8

[0110] The same as Example 4, except that the Rhodotorula glutinosus liquid was replaced with an equal mass fraction of Saccharomyces cerevisiae liquid.

[0111] Comparative Example 9

[0112] Same as Example 1, except that the pepper seed meal is replaced with corn flour of equal mass.

[0113] (1) Animal feed, consisting of the following raw materials in parts by weight: 58 parts of corn flour, 12 parts of soybean flour, 10 parts of wheat flour, 12 parts of straw, 12 parts of mixed powder of orange peel and sugarcane bagasse, 3.5 parts of Jerusalem artichoke powder, 1.5 parts of vanilla powder, 6 parts of fermented soybean meal and 1.2 parts of composite microorganisms.

[0114] The composite microorganism is composed of the following components in parts by mass: 4 parts of Bacillus licheniformis liquid, 5 parts of Bacillus megaterium liquid, 2 parts of Lactobacillus acidophilus liquid and 6 parts of Candida krusei liquid.

[0115] (2) Preparation method of orange peel and bagasse mixed powder

[0116] Fresh orange peel, sugarcane bagasse and water are mixed in a mass ratio of 1:10:5, placed in a container and steamed (steaming temperature is 105°C, time is 12 minutes), then dried (drying temperature is 80°C), and crushed to 300 mesh to obtain orange peel and sugarcane bagasse mixed powder.

[0117] (3) Preparation method of fermented soybean meal

[0118] The soybean meal was crushed into 40 meshes to obtain soybean meal powder, and the soybean meal powder was evenly mixed with water and compound probiotics (the mass ratio of soybean meal, water and compound probiotics was 35:15:1.2), and sealed and fermented (the sealed fermentation temperature was 32°C and the time was 10 days) to obtain fermented soybean meal.

[0119] Among them, the composite probiotics are composed of the following components in parts by mass: 3.5 parts of Bacillus subtilis liquid, 2 parts of Lactobacillus plantarum liquid and 6 parts of Bifidobacterium longum liquid.

[0120] (4) The straw and fermented soybean meal are mixed evenly and then crushed to 300 mesh, and then mixed evenly with the remaining raw materials, and granulated at low temperature (temperature of 40°C, particle size of 1 mm) to obtain animal feed.

[0121] Effect Example 1

[0122] 650 one-day-old white-feathered broiler chickens weighing 38-42 g were selected and divided into 13 treatments using a single-factor completely randomized block design, with 5 replicates per treatment and 10 chickens per replicate. They were fed the animal feed prepared in Examples 1-4 and Comparative Examples 1-9, respectively.

[0123] (1) When the chickens were fed to 42 days of age, the body weight of the broilers was measured, and the average daily feed intake, average daily weight gain and feed conversion rate were calculated. The results are shown in Table 1.

[0124] Table 1 Growth performance of white-feathered broiler chickens

[0125]

[0126] (2) At 42 days of age, five chickens were randomly selected from each treatment after fasting for 12 hours. The chickens were weighed and slaughtered, and the breast muscle weight was recorded. The cooking loss of the breast muscle was calculated (the breast muscle was heated in a water bath. When the core temperature reached 70°C, the breast muscle was removed, drained, cooled to room temperature, and weighed. The cooking loss rate was calculated; cooking loss rate = (mass before cooking - mass after cooking) / mass before cooking × 100%).

[0127] Texture profile analysis (TPA) properties and shear force: After steaming, meat samples were cut into 1 cm × 1 cm × 1 cm pieces parallel to the muscle fibers. TPA properties and shear force were measured using a texture analyzer. This was repeated three times, and the average values ​​were calculated. The results are shown in Table 2.

[0128] Table 2 Quality of breast muscle

[0129]

[0130] As can be seen from Table 2, feeding the animal feed prepared in Example can reduce the hardness, stickiness, chewiness, and shear force of chicken, and can effectively inhibit fat oxidation in meat, thereby improving the tenderness of meat; feeding the animal feed prepared in Example can reduce the cooking loss rate and improve the water retention of chicken.

[0131] (3) The amino acid content in pectoral muscle (fresh pectoral muscle) was determined according to the method specified in the National Food Safety Standard for Determination of Amino Acids in Food (GB5009.124-2016). The results are shown in Table 3.

[0132] Table 3 Amino acid content in pectoral muscle

[0133]

[0134]

[0135] As can be seen from Table 3, feeding the animal feed prepared in Example can increase the amino acid content in chicken.

[0136] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. An animal feed based on Zanthoxylum bungeanum seed meal, characterized in that, The invention comprises the following raw materials in parts by weight: 15-20 parts of Sichuan pepper seed meal, 30-40 parts of corn flour, 10-15 parts of soybean flour, 10-12 parts of wheat flour, 10-12 parts of straw, 10-12 parts of mixed powder of orange peel and sugarcane bagasse, 3-4 parts of Jerusalem artichoke powder, 1-1.5 parts of vanilla powder, 5-8 parts of fermented soybean meal and 1-1.5 parts of composite microorganisms.

2. The animal feed according to claim 1, characterized in that The preparation method of the fermented soybean meal comprises the following steps: The soybean meal is crushed and then evenly mixed with water and composite probiotics, and then sealed and fermented to obtain the fermented soybean meal.

3. The animal feed according to claim 2, characterized in that The mass ratio of the soybean meal, water and composite probiotics is (30-40): (10-15): (1-1.5); And / or, the composite probiotic comprises the following components in parts by weight: 3-4 parts of Bacillus subtilis liquid, 2-3 parts of Lactobacillus plantarum liquid, and 5-8 parts of Bifidobacterium longum liquid; And / or, the temperature of the sealed fermentation is 30-35° C., and the time is 8-10 days.

4. The animal feed according to claim 1, characterized in that The composite microorganism comprises the following components in parts by mass: 3 to 5 parts of Bacillus licheniformis liquid, 4 to 6 parts of Bacillus megaterium liquid, 2 to 3 parts of Lactobacillus acidophilus liquid and 4 to 6 parts of Candida krusei liquid.

5. The animal feed according to claim 1, characterized in that The method for preparing the orange peel and bagasse mixed powder comprises the following steps: Fresh orange peel, sugarcane bagasse and water are mixed in a mass ratio of 1:10:5, steamed, dried and crushed to obtain the orange peel and sugarcane bagasse mixed powder.

6. The animal feed according to claim 5, characterized in that The steaming temperature is 95-105° C. and the steaming time is 10-15 minutes; And / or, the drying temperature is 70-80°C.

7. A method for preparing animal feed according to any one of claims 1 to 6, characterized in that: The following steps are involved: (1) steam explosion treatment is performed on the Zanthoxylum bungeanum seed meal to obtain pretreated Zanthoxylum bungeanum seed meal; (2) The pretreated pepper seed meal, straw and fermented soybean meal are mixed evenly and then crushed, mixed evenly with the remaining raw materials, and granulated at low temperature to obtain the animal feed.

8. The preparation method according to claim 7, characterized in that The steam explosion treatment was performed at a steam pressure of 1.3 MPa, a material moisture content of 35%, and a pressure maintenance time of 140 s.

9. The preparation method according to claim 7, characterized in that Step (1) specifically comprises: sequentially performing steam explosion treatment and fermentation treatment on the Zanthoxylum bungeanum seed meal to obtain pretreated Zanthoxylum bungeanum seed meal; And / or, the fermentation temperature is 30-40°C and the time is 12-24 hours; And / or, the fermentation bacteria used in the fermentation treatment is Rhodotorula glutinosus.

10. Use of the animal feed according to any one of claims 1 to 6 as feed for livestock and poultry breeding.

Images