Experimental mouse intestinal flora sampling device

Abstract

The invention belongs to the technical field of intestinal flora sampling, and particularly relates to an experimental mouse intestinal flora sampling device which comprises a mounting cylinder, the side wall of the mounting cylinder is fixedly connected with a controller and a buzzer, the right side of the mounting cylinder is of an open structure, and the inner wall of the left side of the mounting cylinder is connected with an annular plate through a spring. And an injector is inserted into the right side of the mounting cylinder, a mounting frame is fixedly connected to the front side wall of the mounting cylinder, and the mounting frame is of a U-shaped structure. When the insertion head of the sampling device is inserted from the anus of the mouse, the diameter of the insertion head is reduced, so that the problems that the insertion head is too thick, the mouse is severely painful, the stress hormone level of the mouse is promoted to rise, and the intestinal flora composition is interfered are solved; and the insertion head can be expanded to a proper diameter, so that the problem that the insertion head is blocked by dietary fibers in intestinal tracts of mice is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of intestinal flora sampling, and in particular relates to a device for sampling intestinal flora of experimental mice. Background Art

[0002] Although the intestinal flora of mice is different from that of humans, the intestinal physiological structure and metabolic function are somewhat similar to those of humans. The mouse rectal flora obtained by anal sampling can be used as a simplified model for studying the human distal colon flora. For example, an animal intestinal flora sampling device is proposed in patent publication number CN207575170U.

[0003] During the intestinal flora sampling operation in mice, the sampling device needs to be inserted into the rectum through the anus, and an appropriate amount of saline is injected before the mixed contents are extracted. During this process, if the diameter of the insertion head is too large, it will cause severe pain to the mouse, prompting an increase in the level of stress hormones (such as cortisol), thereby interfering with the composition of the intestinal flora, resulting in the sample being unable to truly reflect the flora characteristics under normal physiological conditions; if the diameter of the insertion head is too small, the undigested food fiber or mucus in the mouse rectal contents will easily block the lumen, causing sampling failure.

[0004] Therefore, an experimental mouse intestinal flora sampling device is proposed to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a device for sampling intestinal flora of experimental mice in order to solve the above problems.

[0006] To achieve the above objectives, the present invention adopts the following technical solutions: an experimental mouse intestinal flora sampling device, comprising a mounting tube, a controller and a buzzer fixedly connected to the side wall of the mounting tube, an open structure on the right side of the mounting tube, a ring plate connected to the left inner wall of the mounting tube via a spring, a syringe plugged into the right side of the mounting tube, a mounting frame fixedly connected to the front side wall of the mounting tube, the mounting frame having a U-shaped structure, and further comprising:

[0007] an insertion assembly, disposed at the left end of the mounting frame, capable of being inserted into the anus of a mouse and extracting rectal contents;

[0008] a collecting assembly, rotatably connected between the insertion assembly and the mounting barrel, for collecting rectal contents at different locations;

[0009] The limiting assembly is arranged on the side wall of the mounting barrel and is used to fix the position of the syringe.

[0010] Preferably, the insertion component includes a circular ring, which is fixedly connected to the left end of the circular ring and the mounting frame, and a rubber ring is fixedly connected to the inner wall of the circular ring, and a plurality of rubber sheets and expansion sheets are fixedly connected to the inner wall of the rubber ring, and the plurality of rubber sheets and expansion sheets are alternately placed, and the rubber sheets and expansion sheets are fixedly connected, and a plurality of lifting chambers are opened inside the circular ring, and the inner wall of the lifting chamber is connected to a piston plate through a spring, and the side wall of the piston plate away from the spring is fixedly connected to a connecting frame, and the side wall of the lifting chamber is opened with a sliding port that matches the connecting frame, and the upper end of the connecting frame extends out of the sliding port and is fixedly connected to the corresponding expansion sheet, and an annular cavity is opened inside the circular ring, and the annular cavity and the lifting chamber are connected to each other, and the side wall of the mounting frame is connected with a booster assembly, and the booster assembly is connected to the annular cavity.

[0011] Preferably, the boosting assembly includes a boosting cylinder fixedly connected to the side wall of the mounting frame, a pressing rod is movably inserted into the right side wall of the boosting cylinder, one end of the pressing rod located in the boosting cylinder is fixedly connected to a piston block, the boosting cylinder and the annular cavity are fixedly connected by a same boosting pipe, and a one-way valve is provided in the boosting pipe.

[0012] Preferably, the collecting assembly includes a rotating shaft rotatably connected between the mounting cylinder and the circular ring, the outer wall of the rotating shaft is fixedly sleeved with two rotating plates, the right side walls of the two rotating plates are fixedly connected to a plurality of cylinders, the right side wall of the rotating plate is fixedly connected to a movable plate located in the cylinder by a spring, the side wall of the movable plate is fixedly plugged with a square tube, the right side wall of the circular ring is fixedly connected to a connecting cover, the right side wall of the connecting cover is fixedly connected with a short tube, the end of the square tube on the left side close to the short tube is fixedly connected with a short tube that matches the short tube, and the right end of the square tube on the right side is fixedly connected with an insert that matches the syringe head, a plurality of collecting tubes are provided between the two rotating plates, the collecting tubes can be rotatably opened, the end of the square tube close to the collecting tube is fixedly connected with a threaded tube, the left and right ends of the collecting tube are fixedly connected with threaded tubes that mesh with the threaded tube, and a pressure relief valve is provided in the threaded tube.

[0013] Preferably, the limiting assembly includes a limiting cylinder fixedly inserted in the side wall of the mounting cylinder, a limiting frame movably inserted in the side wall of the limiting cylinder, a same spring fixedly connected between the limiting frame and the limiting cylinder, one end of the limiting frame located in the limiting cylinder is fixedly connected to a limiting tooth block, and the outer wall of the syringe is fixedly sleeved with a tooth ring that meshes with the limiting tooth block.

[0014] Preferably, the left side wall of the mounting cylinder is fixedly connected to an annular seat via a bracket, a plurality of grooves are provided on the left side wall of the annular seat, and the inner wall of the groove is fixedly connected to an arc seat via a spring, and a plurality of arc grooves matching the arc seat are provided on the side wall of the rotating plate on the right side.

[0015] Preferably, the wall of the boost pipe is fixedly connected to an air pressure pipe, the end of the air pressure pipe is plugged with a blockage, the wall of the annular cavity is provided with an air pressure cavity, and a blockage is plugged into the air pressure cavity.

[0016] Preferably, a pressure sensor is connected to the side wall of one of the expansion pieces, and the pressure sensor is electrically connected to the buzzer through a controller.

[0017] Compared with existing technologies, the advantages of an experimental mouse intestinal flora sampling device are:

[0018] 1. Through the provided insertion component, when the insertion head of the sampling device is inserted into the anus of the mouse, the diameter of the insertion head is made thinner, thereby avoiding the problem that the insertion head is too thick, causing severe pain to the mouse, increasing the level of stress hormones in the mouse, and interfering with the composition of the intestinal flora. After the insertion head is inserted into the anus of the mouse, the insertion head can be expanded to an appropriate diameter, avoiding the problem of the insertion head being blocked by dietary fiber in the mouse intestine.

[0019] 2. Through the set collection component, different collection tubes can be easily replaced when sampling the contents of different locations in the mouse rectum, which is convenient for operators to subsequently study the differences in bacterial flora in different locations in the mouse intestine, ensuring the accuracy of the samples and the scientific nature of the research.

[0020] 3. By setting the annular seat and the arc seat, when collecting the contents at different positions of the mouse rectum, the operator can judge the placement position of the collection tube during the rotation of the collection tube. The operator does not need to deliberately align the collection tube and the syringe, which improves the convenience of use for the operator. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic structural diagram of a device for sampling intestinal flora of experimental mice provided by the present invention;

[0022] Figure 2 This is a schematic structural diagram of a collection component in a device for sampling intestinal flora of experimental mice provided by the present invention;

[0023] Figure 3 This is a schematic structural diagram of an insertion component in an experimental mouse intestinal flora sampling device provided by the present invention;

[0024] Figure 4 This is a right side cross-sectional view of a ring in an experimental mouse intestinal flora sampling device provided by the present invention;

[0025] Figure 5 This is a schematic structural diagram of a pressurizing component in a device for sampling intestinal flora of experimental mice provided by the present invention;

[0026] Figure 6 This is a schematic structural diagram of a limiting component in an experimental mouse intestinal flora sampling device provided by the present invention;

[0027] Figure 7 This is a schematic diagram of the internal structure of an annular seat in an experimental mouse intestinal flora sampling device provided by the present invention.

[0028] In the figure: 1 mounting cylinder, 2 controller, 3 buzzer, 4 ring plate, 5 syringe, 6 mounting frame, 7 insert assembly, 71 ring, 72 rubber ring, 8 rubber sheet, 9 expansion sheet, 10 lifting chamber, 11 piston plate, 12 connecting frame, 13 annular chamber, 14 boosting assembly, 141 boosting cylinder, 142 pressing rod, 15 piston block, 16 boosting pipe, 17 one-way valve, 18 collecting assembly, 181 rotating shaft, 182 Rotating plate, 19 cylinder, 20 moving plate, 21 square tube, 22 connecting cover, 23 short tube, 24 short tube, 25 insert tube, 26 collecting tube, 27 threaded tube, 28 threaded tube, 29 pressure relief valve, 30 limit assembly, 301 limit tube, 302 limit frame, 31 limit gear block, 32 gear ring, 33 annular seat, 34 arc seat, 35 air pressure tube, 36 plug, 37 air pressure chamber, 38 block, 39 pressure sensor. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0030] like Figure 1-Figure 7 As shown, an experimental mouse intestinal flora sampling device includes a mounting tube 1, a controller 2 and a buzzer 3 are fixedly connected to the side wall of the mounting tube 1, the right side of the mounting tube 1 is an open structure, the left inner wall of the mounting tube 1 is connected to a ring plate 4 via a spring, a syringe 5 is plugged into the right side of the mounting tube 1, and a mounting frame 6 is fixedly connected to the front side wall of the mounting tube 1. The mounting frame 6 is a U-shaped structure and further includes:

[0031] The insertion component 7 is arranged at the left end of the mounting frame 6 and can be inserted into the mouse anus and extract the rectal contents. The insertion component 7 includes a circular ring 71, which is fixedly connected to the left end of the mounting frame 6. The inner wall of the circular ring 71 is fixedly connected to a rubber ring 72. The inner wall of the rubber ring 72 is fixedly connected to a plurality of rubber sheets 8 and expansion sheets 9. The plurality of rubber sheets 8 and expansion sheets 9 are alternately placed, and the rubber sheets 8 and expansion sheets 9 are fixedly connected. A plurality of lifting chambers 10 are opened inside the circular ring 71. The inner wall of the lifting chamber 10 is connected to a piston plate 11 through a spring. The side wall of the piston plate 11 away from the spring is fixedly connected to a connecting frame 12. The side wall of the lifting chamber 10 is opened with a sliding port that matches the connecting frame 12. The upper end of the connecting frame 12 extends out of the sliding port and is fixedly connected to the corresponding expansion piece 9. An annular cavity 13 is opened inside the circular ring 71. The annular cavity 13 and the lifting cavity 10 are communicated with each other. The side wall of the mounting frame 6 is connected to a booster assembly 14. The booster assembly 14 is communicated with the annular cavity 13. The booster assembly 14 includes a booster cylinder 141 fixedly connected to the side wall of the mounting frame 6. A pressing rod 142 is movably inserted into the right side wall of the booster cylinder 141. One end of the pressing rod 142 located in the booster cylinder 141 is fixedly connected to a piston block 15. The booster cylinder 141 and the annular cavity 13 are fixedly connected with the same booster pipe 16. A one-way valve 17 is provided in the booster pipe 16, which can change the size of the structure inserted into the mouse anus;

[0032] The collecting assembly 18 is rotatably connected between the insertion assembly 7 and the mounting cylinder 1 and is used to collect rectal contents at different positions. The collecting assembly 18 includes a rotating shaft 181 rotatably connected between the mounting cylinder 1 and the ring 71. The outer wall of the rotating shaft 181 is fixedly sleeved with two rotating plates 182. The right side walls of the two rotating plates 182 are fixedly connected to multiple cylinders 19. The right side wall of the rotating plate 182 is fixedly connected to a movable plate 20 located in the cylinder 19 through a spring. The side wall of the movable plate 20 is fixedly plugged with a square tube 21. The right side wall of the ring 71 is fixedly connected to a connecting cover 22. The right side wall of the connecting cover 22 is fixedly connected to a short tube 23. One end of the square tube 21 on the left side near the short tube 23 is fixedly connected A short tube 24 is provided which matches the short tube 23. The right end of the square tube 21 on the right side is fixedly connected to an insert tube 25 which matches the head of the syringe 5. A plurality of collecting tubes 26 are provided between the two rotating plates 182. The collecting tubes 26 can be rotated to open. One end of the square tube 21 close to the collecting tube 26 is fixedly connected to a threaded tube 27. The left and right ends of the collecting tube 26 are fixedly connected to threaded tubes 28 which mesh with the threaded tube 27. A pressure relief valve 29 is provided in the threaded tube 28. When sampling the contents at different positions of the mouse rectum, different collecting tubes 26 can be easily replaced, which facilitates the operator to conduct subsequent research on the differences in the flora at different positions in the mouse intestine, ensuring the accuracy of the sample and the scientific nature of the research.

[0033] The limiting assembly 30 is arranged on the side wall of the mounting barrel 1 and is used to fix the position of the syringe 5. The limiting assembly 30 includes a limiting barrel 301 fixedly inserted into the side wall of the mounting barrel 1, and a limiting frame 302 is movably inserted into the side wall of the limiting barrel 301. The limiting frame 302 and the limiting barrel 301 are fixedly connected with the same spring. One end of the limiting frame 302 located in the limiting barrel 301 is fixedly connected to the limiting tooth block 31. The outer wall of the syringe 5 is fixedly sleeved with a tooth ring 32 that meshes with the limiting tooth block 31, which can fix the syringe 5 in the mounting barrel 1.

[0034] The left side wall of the mounting cylinder 1 is fixedly connected to an annular seat 33 through a bracket, a plurality of grooves are provided on the left side wall of the annular seat 33, and an arc seat 34 is fixedly connected to the inner wall of the groove through a spring, and a plurality of arc grooves matching the arc seat 34 are provided on the side wall of the right rotating plate 182, so that the operator can place the collection tube 26 conveniently.

[0035] The wall of the boost pipe 16 is fixedly connected to an air pressure pipe 35, and a plug 36 is inserted at the end of the air pressure pipe 35. The wall of the annular cavity 13 is provided with an air pressure cavity 37, and a block 38 is inserted in the air pressure cavity 37 to facilitate the discharge of the gas inside the annular cavity 13 and allow external gas to enter the boost pipe 16.

[0036] A pressure sensor 39 is connected to the side wall of one of the expansion pieces 9. The pressure sensor 39 is electrically connected to the buzzer 3 through the controller 2 and can detect the pressure between the expansion piece 9 and the inner wall of the mouse rectum.

[0037] The operating principle of the present invention is now described as follows: the collecting tube 26 is rotated to open, and then an appropriate amount of physiological saline is placed in the collecting tube 26. Next, the collecting tube 26 is rotated to close, and then the threaded tubes 28 at both ends of the collecting tube 26 are pressed against the threaded barrels 27 on both sides in turn. Under the action of the spring force, the movable plate 20 drives the threaded barrel 27 to move outward, and after the threaded tube 28 is aligned with the threaded barrel 27, a certain pressure is generated between the threaded barrel 27 and the threaded tube 28 under the action of the spring force. Then, the collecting tube 26 is rotated, and the collecting tube 26 drives the threaded tube 28 to rotate. Through the meshing of the threaded tube 28 and the threaded barrel 27, the threaded tube 28 and the threaded barrel 27 are connected together, thereby connecting the collecting tube 26. Then, according to this method, multiple collecting tubes 26 are installed in sequence between the two rotating plates 182, thereby installing multiple collecting tubes 26.

[0038] Next, the operator rotates the rotating plate 182 so that the rotating plate 182 drives the collecting tube 26 and the two square tubes 21 to rotate to a suitable position. In the process of rotation, the position of the rotating plate 182 can be fixed by the arc seat 34 and the arc groove on the side wall of the rotating plate 182, thereby fixing the positions of the collecting tube 26 and the square tube 21. Next, the operator inserts the syringe 5 into the mounting barrel 1, and makes the head of the syringe 5 pass through the hole on the surface of the mounting barrel 1 and into the inserting barrel 25. Then, the operator squeezes the syringe 5 so that the syringe 5 drives the gear ring 32 to pass through the limiting tooth block 31. When the gear ring 32 passes through the limiting tooth block 31, the gear ring 32 is inserted into the mounting barrel 1. 2 and the inclined surface of the limiting tooth block 31 cooperate with each other, so that the limiting tooth block 31 drives the limiting frame 302 to move outward to a certain position, and after the limiting tooth block 31 and the gear ring 32 are separated, the limiting frame 302 will drive the limiting tooth block 31 to return to its original position under the action of the spring force, and fix the position of the gear ring 32 and the syringe 5, and when the syringe 5 is inserted, the syringe 5 will be inserted into the insertion tube 25 and squeeze the square tube 21 on the right, and the square tube 21 on the right will drive the collecting tube 26, the square tube 21 on the left and the short tube 24 to move to the left together, so that the short tube 24 is plugged into the outside of the short tube 23, so that the syringe 5, the collecting tube 26 and the insertion component 7 are connected;

[0039] Next, the operator takes out the mouse and places it with its head facing down and its anus facing up, then inserts multiple rubber sheets 8 and expansion sheets 9 into the set positions of the mouse's anus, and then the operator squeezes the pressing rod 142, which drives the piston block 15 to move in the boosting cylinder 141, so that the gas in the boosting cylinder 141 is transported to the annular cavity 13 through the boosting pipe 16, and is transported to one side of the piston plate 11 in the lifting cavity 10 through the annular cavity 13. Under the action of the air pressure, the piston plate 11 drives the connecting frame 12 to move together, and the connecting frame 12 drives the expansion sheets 9 to move, and multiple expansion sheets 9 move outward. The expansion piece 9 moves and stretches the rubber piece 8, thereby expanding the part inserted into the mouse anus. In the process of moving outward, the expansion piece 9 will drive the pressure sensor 39 to move together. After the pressure sensor 39 detects that the squeezing force between the expansion piece 9 and the inner wall of the mouse rectum reaches the set threshold value (0.3N), the pressure sensor 39 will drive the buzzer 3 to work through the controller 2. When the operator hears the buzzer 3 working, he needs to stop squeezing the pressing rod 142, and then the operator first presses the syringe 5 to allow the gas inside the syringe 5 to enter the receiving tube through the right square tube 21, the threaded cylinder 27, and the threaded tube 28. The pressure in the collecting tube 26 is increased (when the gas in the syringe 5 exceeds the threshold of the right pressure relief valve 29, the gas will pass through the pressure relief valve 29). Under the action of pressure (when the water pressure in the collecting tube 26 exceeds the threshold of the left pressure relief valve 29, the saline will pass through the left pressure relief valve 29), it will be transported to the space between the multiple rubber sheets 8 and the expansion sheet 9 through the left threaded tube 28, square tube 21, short tube 23 and connecting cover 22, and then transported to the rectum of the mouse. The operator needs to press the mouse's abdomen for ten seconds to mix the saline with the intestinal contents, and then The operator then pulls out the syringe 5, and referring to the above principle, the physiological saline and intestinal contents will be extracted into the collecting tube 26 for storage. When it is necessary to sample the contents deeper in the mouse intestine, it is only necessary to replace a different collecting tube 26 (refer to the above principle for the replacement steps) and push the device again to move the rubber sheet 8 and the expansion sheet 9 to the deep part of the mouse intestine to sample the deeper contents. The subsequent operator only needs to rotate the collecting tube 26 out of the threaded barrel 27, and then rotate open the collecting tube 26 to take out the contents and use other detection equipment to detect the intestinal flora.

[0040] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An experimental mouse intestinal flora sampling device, comprising a mounting tube (1), a controller (2) and a buzzer (3) fixedly connected to the side wall of the mounting tube (1), the right side of the mounting tube (1) is an open structure, the left inner wall of the mounting tube (1) is connected to a ring plate (4) through a spring, a syringe (5) is plugged into the right side of the mounting tube (1), and a mounting frame (6) is fixedly connected to the front side wall of the mounting tube (1), and the mounting frame (6) is a U-shaped structure, characterized in that Also includes: An insertion assembly (7), disposed at the left end of the mounting frame (6), capable of being inserted into the anus of a mouse and extracting rectal contents; a collecting assembly (18) rotatably connected between the insertion assembly (7) and the mounting cylinder (1) for collecting rectal contents at different locations; A limiting assembly (30) is arranged on the side wall of the mounting cylinder (1) and is used to fix the position of the syringe (5).

2. The experimental mouse intestinal flora sampling device according to claim 1, characterized in that: The insert assembly (7) includes a circular ring (71), the circular ring (71) is fixedly connected to the left end of the mounting frame (6), the inner wall of the circular ring (71) is fixedly connected to a rubber ring (72), the inner wall of the rubber ring (72) is fixedly connected to a plurality of rubber sheets (8) and expansion sheets (9), the plurality of rubber sheets (8) and expansion sheets (9) are alternately placed, and the rubber sheets (8) and expansion sheets (9) are fixedly connected to each other, the interior of the circular ring (71) is provided with a plurality of lifting chambers (10), the inner wall of the lifting chamber (10) is connected to a piston plate ( 11), the side wall of the piston plate (11) away from the spring is fixedly connected to a connecting frame (12), the side wall of the lifting chamber (10) is provided with a sliding opening that matches the connecting frame (12), the upper end of the connecting frame (12) extends out of the sliding opening and is fixedly connected to the corresponding expansion piece (9), the interior of the ring (71) is provided with an annular cavity (13), the annular cavity (13) and the lifting chamber (10) are communicated with each other, the side wall of the mounting frame (6) is connected to a booster assembly (14), and the booster assembly (14) and the annular cavity (13) are communicated.

3. The experimental mouse intestinal flora sampling device according to claim 2, characterized in that: The boosting assembly (14) includes a boosting cylinder (141) fixedly connected to the side wall of the mounting frame (6); a pressing rod (142) is movably inserted into the right side wall of the boosting cylinder (141); one end of the pressing rod (142) located in the boosting cylinder (141) is fixedly connected to a piston block (15); the boosting cylinder (141) and the annular cavity (13) are fixedly connected by a same boosting pipe (16); a one-way valve (17) is provided in the boosting pipe (16).

4. The experimental mouse intestinal flora sampling device according to claim 1, characterized in that: The collecting assembly (18) includes a rotating shaft (181) rotatably connected between the mounting cylinder (1) and the circular ring (71); the outer wall of the rotating shaft (181) is fixedly sleeved with two rotating plates (182); the right side walls of the two rotating plates (182) are fixedly connected to a plurality of cylinders (19); the right side wall of the rotating plate (182) is fixedly connected to a movable plate (20) located in the cylinder (19) through a spring; the side wall of the movable plate (20) is fixedly plugged with a square tube (21); the right side wall of the circular ring (71) is fixedly connected to a connecting cover (22); the right side wall of the connecting cover (22) is fixedly connected to a short tube (23) located on the left side. One end of the square tube (21) near the short tube (23) is fixedly connected to a short tube (24) that matches the short tube (23), and the right end of the square tube (21) on the right side is fixedly connected to an insert tube (25) that matches the head of the syringe (5). A plurality of collecting tubes (26) are provided between the two rotating plates (182). The collecting tubes (26) can be rotated to open. One end of the square tube (21) near the collecting tube (26) is fixedly connected to a threaded tube (27). The left and right ends of the collecting tube (26) are fixedly connected to threaded tubes (28) that mesh with the threaded tube (27). A pressure relief valve (29) is provided in the threaded tube (28).

5. The experimental mouse intestinal flora sampling device according to claim 1, characterized in that: The limiting assembly (30) includes a limiting cylinder (301) fixedly plugged into the side wall of the installation cylinder (1); the side wall of the limiting cylinder (301) is movably plugged into a limiting frame (302); a same spring is fixedly connected between the limiting frame (302) and the limiting cylinder (301); one end of the limiting frame (302) located in the limiting cylinder (301) is fixedly connected to the limiting tooth block (31); and the outer wall of the syringe (5) is fixedly sleeved with a tooth ring (32) that meshes with the limiting tooth block (31).

6. The experimental mouse intestinal flora sampling device according to claim 4, characterized in that: The left side wall of the mounting cylinder (1) is fixedly connected to an annular seat (33) via a bracket, the left side wall of the annular seat (33) is provided with a plurality of grooves, and the inner wall of the groove is fixedly connected to an arc seat (34) via a spring, and the side wall of the rotating plate (182) on the right side is provided with a plurality of arc grooves matching the arc seat (34).

7. The experimental mouse intestinal flora sampling device according to claim 3, characterized in that: The wall of the boost pipe (16) is fixedly connected to an air pressure pipe (35), the end of the air pressure pipe (35) is plugged with a plug (36), the wall of the annular cavity (13) is provided with an air pressure cavity (37), and a block (38) is plugged into the air pressure cavity (37).

8. The experimental mouse intestinal flora sampling device according to claim 2, characterized in that: A pressure sensor (39) is connected to the side wall of one of the expansion pieces (9), and the pressure sensor (39) is electrically connected to the buzzer (3) via the controller (2).

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