Construction tool and method for mouse jugular vein intubation model

Abstract

The invention provides a construction tool and method for a mouse jugular vein intubation model, and relates to the technical field of medical tests. The tool comprises a trocar and a catheter, the trocar comprises a handle part and a needle head which are integrally connected; the trocar is of a hollow needle tube structure; the catheter can be arranged in the trocar in a penetrating manner; the end part of the conduit is a flat head; a groove is formed in the end, where the needle head is located, of the trocar. During intubation, the catheter is sleeved with the tool, the vein can not be opened, the head of the trocar needle point is directly used for puncturing the vein so that a blood vessel channel for intubation can be established, and after the catheter is brought into the vein, the trocar needle is pulled out. Under the condition that vein opening is not carried out, the visual field of an operator is clearer, the time for intubation can be shortened, and the problems of blood vessel damage, catheter blockage in the later period and the like are solved.

Description

Technical Field

[0001] The present invention relates to the field of medical experiment technology, and in particular to a tool and method for constructing a mouse jugular vein cannulation model. Background Art

[0002] The development of mouse jugular vein cannulation technology is closely related to the needs of medical research. This technology allows experimental animals to remain awake and move freely during blood collection, reducing interference or stimulation to the animals, thereby obtaining more accurate and reasonable experimental results. Jugular vein cannulation is an important animal experimental method, widely used in long-term intravenous drug administration, blood collection, and other studies, such as venous pressure measurement, pharmacokinetic studies, and glucose clamp experiments. As experimental animals, mice have become one of the most common experimental animals due to their small size, low price, and convenient and quick operation. Due to the small size of mice and the extremely thin jugular vein, cannulation is difficult. Compared with larger rodent experimental animals such as rabbits and rats, there are fewer reports on mouse jugular vein cannulation methods. Therefore, improving and promoting mouse jugular vein cannulation methods is of great significance to scientific researchers.

[0003] Successfully establishing a mouse jugular vein cannulation model facilitates long-term intravenous drug administration and blood collection in mice, which is particularly important in research fields such as drug addiction, physiology, and pharmacology.

[0004] Problems with existing technologies for mouse jugular vein cannulation models:

[0005] 1. After the vein is opened, blood flows out quickly, causing vasoconstriction. This makes the incision field unclear and inconvenient for cannulation, resulting in prolonged surgery and even vascular damage.

[0006] 2. If the cannula is trimmed into a pointed tip, it is relatively easy to insert into the blood vessel, but later the bevel of the catheter will easily adhere to the inner wall of the blood vessel, causing congestion in the catheter.

[0007] Based on this, the present application proposes a tool and method for constructing a mouse jugular vein cannulation model, which can effectively reduce the surgical time of jugular vein cannulation, reduce the mortality rate of mice, and avoid the problem of catheter adhesion and blockage to the blood vessel wall in the later stage. Summary of the Invention

[0008] The present invention aims to overcome the shortcomings of the prior art and provides a tool and method for constructing a mouse jugular vein cannulation model, which is specifically achieved through the following technical solutions:

[0009] The present invention also provides a tool for constructing a mouse jugular vein cannulation model, comprising a trocar and a catheter;

[0010] The trocar includes an integrally connected handle and a needle; the trocar is a hollow needle tube structure; the catheter can be inserted into the trocar; the end of the catheter is flat; and a groove is provided at the end where the needle is located.

[0011] Optionally or preferably, the distance between the groove and the end of the needle is 1.5-0.5 cm.

[0012] Optionally or preferably, the outer wall of the catheter near the insertion end is further provided with expansion fins for supporting the vein.

[0013] The present invention also provides a method for constructing a mouse jugular vein cannulation model, based on the above-mentioned mouse jugular vein cannulation model construction tool, comprising the following steps:

[0014] S1. Anesthetize the mouse, remove the hair on the mouse skin, and disinfect the mouse skin

[0015] S2. Fix the mouse's limbs on the operating table in a supine position;

[0016] S3, disinfecting the construction tool;

[0017] S4. Make a longitudinal incision on the side of the mouse's neck;

[0018] S5, separate the fat and free the external jugular vein;

[0019] S6, pass a double strand of sutures inferiorly between the confluence of the transverse jugular vein and the suprascapular vein;

[0020] S7, tie the distal end and leave a slipknot at the proximal end to secure the catheter;

[0021] S8. Insert the catheter in the construction tool into the mouse's vein through the needle of the cannula;

[0022] S9. Tie the distal ligature again behind the nested tube and tighten the slipknot at the proximal end to prevent the catheter from slipping out.

[0023] S10, remove the trocar from the vein;

[0024] S11. Turn the mouse over and make a longitudinal incision on its back.

[0025] S12: A subcutaneous tunnel is opened between the anterior cervical incision and the dorsal incision. The free end of the catheter is passed through the subcutaneous tunnel and out of the dorsal opening. The catheter is shortened and resealed with a heparin cap.

[0026] S13. Suture the incision, prepare the skin again and disinfect it, then place the mouse back in the cage in a side-lying position and house it individually.

[0027] Optionally or preferably, the step S1 specifically includes:

[0028] S101, inject 0.1-0.12 mL / 100 g of Shutai combined with xylazine into the abdominal cavity of mice;

[0029] S102. Shave the hair on the side of the mouse's neck and behind the ears with an electric shaver. S103. Disinfect the epidermis with 75% ethanol and iodine disinfectant.

[0030] Optionally or preferably, step S4 specifically comprises: using ophthalmic scissors to make a 0.8-1.2 cm long longitudinal incision above the clavicle of the mouse, 1 cm to the right of the anterior midline of the neck.

[0031] Optionally or preferably, the step S11 specifically includes: after the intubation is completed, turning the mouse over, and making a longitudinal incision 0.4-0.6 cm long at the middle of the neck and back of the mouse.

[0032] Based on the above technical solution, the following technical effects can be produced:

[0033] The present invention provides a tool and method for constructing a mouse jugular vein cannulation model, which can shorten surgical time, reduce animal stress, lower animal mortality, and reduce catheter congestion rate. The construction method is simple and quick to operate, reduces the variability of experimental personnel operations, and makes later experimental data more stable and reliable. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0035] Figure 1 Schematic diagram of the structure of the trocar in the present invention;

[0036] Figure 2 Schematic diagram of the structure of the catheter in the present invention;

[0037] Figure 3 This is a real picture of the method of the present invention;

[0038] In the figure: 1- handle, 2- needle, 3- groove, 4- expansion fin. DETAILED DESCRIPTION

[0039] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0041] Example 1:

[0042] like Figure 1-Figure 2 As shown:

[0043] The present invention provides a tool for constructing a mouse jugular vein cannulation model, comprising a trocar and a catheter;

[0044] The trocar includes an integrally connected handle 1 and a needle 2; the trocar is a hollow needle tube structure; the catheter can be inserted into the trocar; the end of the catheter 3 is a flat head; a groove 3 is provided at the end where the needle 2 of the trocar is located.

[0045] In this embodiment, the distance between the groove 3 and the end of the needle is 1 cm.

[0046] In this embodiment, an expansion fin 4 is further provided on the outer wall of the catheter near the insertion end for supporting the vein.

[0047] like Figure 3 As shown, this embodiment also provides a method for constructing a mouse jugular vein cannulation model, using the above-mentioned construction tool, comprising the following steps:

[0048] S1. Anesthetize the mouse, remove the hair on the mouse skin, and disinfect the mouse skin

[0049] S2. Fix the mouse's limbs on the operating table in a supine position;

[0050] S3, disinfecting the construction tool;

[0051] S4. Use ophthalmic scissors to make a 1-cm-long longitudinal incision above the clavicle of the mouse, 1 cm to the right of the anterior midline of the neck.

[0052] S5, separate the fat and free the external jugular vein;

[0053] S6, pass a double strand of sutures inferiorly between the confluence of the transverse jugular vein and the suprascapular vein;

[0054] S7, tie the distal end and leave a slipknot at the proximal end to secure the catheter;

[0055] S8. Insert the catheter in the construction tool into the mouse's vein through the needle of the cannula;

[0056] S9. Tie the distal ligature again behind the nested tube and tighten the slipknot at the proximal end to prevent the catheter from slipping out.

[0057] S10, remove the trocar from the vein;

[0058] S11. After intubation, turn the mouse over and make a 0.5 cm long longitudinal incision in the middle of the neck and back of the mouse.

[0059] S12. Use ophthalmic forceps to create a subcutaneous tunnel between the anterior neck incision and the dorsal incision. Pass the free end of the catheter through the subcutaneous tunnel and out of the dorsal opening. Cut the catheter to 1 cm and re-cap it with a heparin cap.

[0060] S13. Suture the incision, prepare the skin again and disinfect it, then place the mouse back in the cage in a side-lying position and house it individually.

[0061] In this embodiment, step S1 specifically includes:

[0062] S101, inject 0.1-0.12 mL / 100 g of Shutai combined with xylazine into the abdominal cavity of mice;

[0063] S102. Shave the hair on the side of the mouse's neck and behind the ears with an electric shaver. S103. Disinfect the epidermis with 75% ethanol and iodine disinfectant.

[0064] The working principle of this embodiment is:

[0065] During intubation, the catheter is inserted into the tool, eliminating the need to open the vein. The tip of the introducer needle can be used to directly penetrate the vein, establishing a vascular channel for cannulation. Once the catheter is inserted into the vein, the introducer needle can be removed. This eliminates the need to open the vein, providing the operator with a clearer field of view, reducing intubation time and preventing vessel damage and subsequent catheter blockage. The blunt end of the catheter prevents congestion caused by the tip contacting the vessel wall. The expansion fins on the catheter support the vessel, preventing the catheter from slipping out during subcutaneous tunneling and suturing.

[0066] This embodiment has the following advantages:

[0067] 1. The operation time can be shortened by 50%, reducing the stress on the animals.

[0068] 2. The mortality rate was reduced from the original 15-20% to 5%.

[0069] 3. The catheter congestion rate dropped from 30% to 10%.

[0070] 4. The time it takes for animals to recover to a healthy state after surgery is shortened.

[0071] 5. The operation is simple and quick, which reduces the differences in the operation of the experimenters and makes the later experimental data more stable and reliable.

[0072] 6. Improved the repeatability of the experiment.

[0073] The foregoing description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein and should not be construed as excluding other embodiments. Rather, the present invention can be used in various other combinations, modifications, and environments and can be modified within the scope of the concept described herein through the above teachings or techniques or knowledge in the relevant field. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention are intended to be protected by the appended claims.

Claims

1. A tool for constructing a mouse jugular vein cannulation model, characterized by: Including trocars and catheters; The trocar includes an integrally connected handle and a needle; the trocar is a hollow needle tube structure; the catheter can be inserted into the trocar; the end of the catheter is flat; and a groove is provided at the end where the needle is located.

2. The tool for constructing a mouse jugular vein cannulation model according to claim 1, characterized in that: The distance between the groove and the end of the needle is 1.5-0.5 cm.

3. The tool for constructing a mouse jugular vein cannulation model according to claim 1, characterized in that: The outer wall of the catheter close to the insertion end is also provided with expansion fins for supporting the veins.

4. A method for constructing a mouse jugular vein cannulation model, characterized by: Using the construction tool as claimed in claim 1, comprising the following steps: S1. Anesthetize the mouse, remove the hair on the mouse skin, and disinfect the mouse skin S2. Fix the mouse's limbs on the operating table in a supine position; S3, disinfecting the construction tool; S4. Make a longitudinal incision on the side of the mouse's neck; S5, separate the fat and free the external jugular vein; S6, pass a double strand of sutures inferiorly between the confluence of the transverse jugular vein and the suprascapular vein; S7, tie the distal end and leave a slipknot at the proximal end to secure the catheter; S8. Insert the catheter in the construction tool into the mouse's vein through the needle of the cannula; S9. Tie the distal ligature again behind the nested tube and tighten the slipknot at the proximal end to prevent the catheter from slipping out. S10, remove the trocar from the vein; S11. Turn the mouse over and make a longitudinal incision on its back. S12: A subcutaneous tunnel is opened between the anterior cervical incision and the dorsal incision. The free end of the catheter is passed through the subcutaneous tunnel and out of the dorsal opening. The catheter is shortened and resealed with a heparin cap. S13. Suture the incision, prepare the skin again and disinfect it, then place the mouse back in the cage in a side-lying position and house it individually.

5. The method for constructing a mouse jugular vein cannulation model according to claim 3, wherein: The step S1 specifically includes: S101, inject 0.1-0.12 mL / 100 g of Shutai combined with xylazine into the abdominal cavity of mice; S102. Shave the hair on the side of the mouse's neck and behind the ears with an electric shaver. S103. Disinfect the epidermis with 75% ethanol and iodine disinfectant.

6. The method for constructing a mouse jugular vein cannulation model according to claim 3, wherein: The step S4 specifically includes: making a 0.8-1.2 cm long longitudinal incision above the clavicle of the mouse 1 cm to the right of the anterior midline of the neck using ophthalmic scissors.

7. The method for constructing a mouse jugular vein cannulation model according to claim 3, wherein: The step S11 specifically includes: after the intubation is completed, the mouse is turned over, and a longitudinal incision of 0.4-0.6 cm in length is made at the middle of the neck and back of the mouse.

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