Brain glioma gene biomarker detection kit
By installing an aluminum box with chilled water, a heat sink, and a semiconductor refrigeration plate inside the test kit, the problem of unstable storage caused by temperature changes after the test kit is taken out is solved, and long-term low-temperature storage is achieved, ensuring the effectiveness of brain glioma gene testing.
Patent Information
- Application Number
- CN202510904768.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-01
- Publication Date
- 2025-10-03
AI Technical Summary
When the glioma gene detection kit is taken out of the refrigerator and exposed to the environment, the temperature change can easily affect the performance of the drug, resulting in unstable storage.
A low-temperature insulation structure is set up in the test kit, including clear water in the aluminum box frozen into ice cubes to provide a low-temperature environment, a heat sink and semiconductor refrigeration sheet between the outer box and the inner box to assist in low-temperature preservation, combined with sponge blocks and cotton ropes to absorb cold water to provide a continuous low-temperature environment.
The test kit can be stored at low temperature for a long time after being taken out, ensuring the stability of the drug performance and being suitable for gene detection of brain glioma.
Smart Images
Figure CN120736100A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of kits, and in particular to a kit for detecting gene biomarkers of brain glioma. Background Art
[0002] Gliomas are the most common primary tumors of the central nervous system in adults, accounting for approximately 50% of malignant brain and other central nervous system tumors. They are characterized by high malignancy, rapid progression, and high recurrence. According to the traditional World Health Organization (WHO) classification, gliomas are roughly divided into four grades based on their histological characteristics, including lower-grade gliomas (LGG) and grade IV glioblastomas (GBM). The annual incidence of brain gliomas in my country is 5-8 per 100,000 people, and the five-year mortality rate is second only to pancreatic cancer and lung cancer among systemic tumors. GBM is the most malignant type of glioma, with a peak incidence in the 30-40 age group, an average progression time of 6.9 months, an average overall survival of only 14.6 months, and a five-year survival rate of less than 3%, placing a heavy burden on individuals, families, and society.
[0003] Glioma gene detection kits can be used to detect glioma genes. However, they need to be stored in a specific low-temperature environment to maintain their stability and activity. They are usually kept frozen below zero degrees Celsius. However, between the time the kit is taken out of the refrigerator and the time the test is performed, the kit is exposed to the environment and is easily affected by the ambient temperature and quickly warms up, affecting the performance of the reagents in the kit. Therefore, we propose a glioma gene biomarker detection kit. Summary of the Invention
[0004] The object of the present invention is to provide a glioma gene biomarker detection kit. By arranging a low-temperature insulation structure inside the inner box, the clean water in the aluminum box can be frozen when stored in the refrigerator, and the reagent can be kept at a low temperature for a long time after being taken out. In addition, a structure is also provided between the outer box and the inner box to assist in low-temperature preservation with the help of external ice cubes, which is conducive to long-term low-temperature storage of the kit when the kit is taken out and not in use, thereby solving the problems raised in the background technology.
[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a glioma gene biomarker detection kit, comprising an inner box, an outer box, an aluminum box and a foam block, the upper sealed cover of the inner box is provided with an upper cover, the outer box is fixed to the outer ring of the inner box, the upper half of the inner box is exposed to the outside of the outer box, and the upper part of the outer box is connected to the outside, a sealing paper is connected and fixed between the inner surface of the upper part of the outer box and the outer surface of the inner box, and an easy-to-tear opening is provided in the middle position of the surface of the sealing paper; a heat sink is fixed on the outer ring of the inner box inside the outer box, the inner surface of the heat sink is in contact with the outer surface of the inner box, and fins are evenly fixed on the surface of the heat sink, and the fins are mutually connected. They are parallel to each other, and sponge strips are filled between adjacent fins, and the bottom of the sponge strips is placed on the bottom of the outer box; an aluminum box is placed at the bottom of the inner box, and the interior of the aluminum box is filled with clean water. A ring frame is fixed to the outer ring above the aluminum box, and the ring frame is in contact with the inner wall surface of the inner box. The bottom of the ring frame is connected to the interior of the aluminum box through a through-hole opened on the outer ring surface of the aluminum box; the foam block is placed above the aluminum box on the inner side of the ring frame, and a plurality of placement grooves are evenly opened on the surface of the foam block, a cotton pad is placed horizontally above the ring frame, and a 96-well plate placed in the inner box is supported above the cotton pad, and a cotton pressing plate is fixed to the bottom of the upper cover, and the cotton pressing plate is pressed against the surface of the 96-well plate.
[0006] By adopting the above technical solution, reagents and detection tools are placed in different placement slots of the foam block for storage. During the process of freezing and storing in the refrigerator, the clean water in the aluminum box will be frozen into ice cubes. When the test kit is taken out, the ice cubes slowly melt to provide a low-temperature storage environment. The melted water seeps into the sponge block or cotton rope, which is beneficial to providing a longer low-temperature storage environment for the bottom and outer ring of the reagent. When the removal and placement time is too long, the ice cubes can also be used to cool the aluminum plate or the hot end of the semiconductor refrigeration plate, which is beneficial to provide a low-temperature storage environment for the inside of the test kit for a longer time and avoid affecting the performance of the reagent.
[0007] Optionally, the heat sink is an aluminum plate, and the fins and the aluminum plate are connected to form an integral structure.
[0008] By adopting the above technical solution, the aluminum plate has a good thermal conductivity and can be cooled by ice cubes to provide a low-temperature environment inside the inner box.
[0009] Optionally, the heat sink is a semiconductor refrigeration plate, the fins are fixed to the hot end surface of the semiconductor refrigeration plate, and the cold end of the semiconductor refrigeration plate is in contact with the outer surface of the inner box.
[0010] By adopting the above technical solution, the semiconductor refrigeration plate has a better cooling effect. Its hot end is cooled by fins and ice cubes, and its inner hot end provides the inner box with a better low-temperature storage environment.
[0011] Optionally, the interior of the ring frame is filled with a water-absorbing structure, and the bottom of the water-absorbing structure extends into the interior of the aluminum box through a through-hole.
[0012] By adopting the above technical solution, clean water can be absorbed into the absorption structure before freezing, providing a low-temperature storage environment for the outer ring of the reagent. After cooling with cold water, it can also be moistened into the water absorption structure due to capillary phenomenon, providing a long-term low-temperature storage environment for the outer ring of the reagent.
[0013] Optionally, the water absorption structure is a sponge block, and the sponge block is evenly filled in the interior of the ring frame.
[0014] By adopting the above technical solution, the sponge block has a good absorption effect on clean water.
[0015] Optionally, the water absorbing structure is a cotton rope, and a plurality of cotton ropes are evenly arranged, and the plurality of cotton ropes extend vertically to the top of the ring frame.
[0016] By adopting the above technical solution, the cotton rope can absorb clean water while reducing the use of materials, and can prevent the water moistened in the sponge block from expanding too much after freezing.
[0017] Optionally, partitions are vertically fixed inside the ring frame between the cotton ropes, and the cotton ropes are placed between the partitions and are vertically bound.
[0018] By adopting the above technical solution, the partitions are used to vertically bind the cotton ropes between adjacent partitions.
[0019] Optionally, bumps are fixed to the four corners of the surface of the upper cover, and the 96-well plate can be placed inside the bumps to be positioned and supported on the surface of the upper cover.
[0020] By adopting the above technical solution, it is convenient to position and place the 96-well plate, and it is convenient to use the 96-well plate for detection work.
[0021] Optionally, a rubber ring is fixedly attached to the inner surface of the upper cover, and the rubber ring contacts the outer surface of the inner box.
[0022] By adopting the above technical solution, after the upper cover is placed on the outer ring above the inner box, it has a better anti-slip effect.
[0023] Compared with the prior art, the technical solution of this application has the following beneficial effects:
[0024] 1. The technical solution of the present application is to provide a low-temperature insulation structure inside the inner box, so that the clean water in the aluminum box can be frozen when stored in the refrigerator, and the reagent can be kept at a low temperature for a long time after being taken out. In addition, a structure is also provided between the outer box and the inner box to assist in low-temperature preservation with the help of external ice cubes, which is conducive to long-term low-temperature storage of the test kit when the test kit is taken out and not used.
[0025] 2. The technical solution of this application uses a heat sink as a semiconductor refrigeration plate. When ice or cold water is used to cool the fins at its hot end, the semiconductor refrigeration plate can provide a lower low-temperature storage environment, which is beneficial for the storage of reagents inside the test kit.
[0026] 3. The technical solution of the present application places a sponge block or cotton rope inside the ring frame, and cold water can wet the sponge block or cotton rope under capillary action, providing a good low-temperature storage environment for the outer circle of the reagent, and the sponge strips between the fins are also conducive to cold water wetting the sponge strips placed between the fins, so as to better cool or dissipate heat for the heat sink.
[0027] 4. The technical solution of the present application has protrusions fixed on the four corners of the surface of the upper cover. After the 96-well plate is taken out, it can be placed between the protrusions and positioned on the surface of the upper cover, which is convenient for positioning and use of the 96-well plate. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Other features, objects and advantages of the present invention will become more apparent upon reading the detailed description of non-limiting embodiments with reference to the following drawings:
[0029] Figure 1 Schematic diagram of the overall structure of the glioma gene biomarker detection kit of the present invention;
[0030] Figure 2 This is a schematic diagram of the structure of the glioma gene biomarker detection kit of the present invention after the sealing paper is torn off;
[0031] Figure 3 Schematic diagram of the internal detailed structure of the glioma gene biomarker detection kit of the present invention;
[0032] Figure 4 This is a schematic structural diagram of Example 3 of the glioma gene biomarker detection kit of the present invention.
[0033] In the figure: 1. Inner box; 11. Cotton pad; 12. 96-well plate; 2. Outer box; 21. Heat sink; 22. Fins; 23. Sealing paper; 231. Easy-tear notch; 24. Sponge strip; 3. Aluminum box; 31. Ring frame; 311. Sponge block; 312. Cotton rope; 313. Partition; 32. Through-hole; 4. Foam block; 41. Placement slot; 5. Upper cover; 51. Bump; 52. Rubber ring; 53. Cotton pressing plate. DETAILED DESCRIPTION
[0034] Example 1
[0035] See also Figure 1-3The present invention provides a technical solution: a glioma gene biomarker detection kit, comprising an inner box 1, an outer box 2, an aluminum box 3 and a foam block 4, wherein the aluminum box 3 is placed at the bottom of the inner box 1 and filled with the bottom of the inner box 1. The interior is filled with clean water, and the foam block 4 is placed in the inner box 1 above the aluminum box 3. A plurality of placement grooves 41 are evenly opened on the surface of the foam block 4. Test tubes for placing various reagents can be placed in the placement grooves 41, and other tools used can be placed in other placement grooves 41. An upper cover 5 is placed on the upper part of the inner box 1 to seal the top of the inner box 1. In order to prevent the inner box 1 from slipping, a rubber ring 52 is fixed on the inner ring surface of the upper cover 5. The rubber ring 52 is in contact with the outer surface of the inner box 1, and then a plastic sealing process is performed to ensure the internal sealing of the inner box 1.
[0036] When stored in a refrigerator, the clean water inside the aluminum box 3 will be frozen into ice cubes. When the inner box 1 is taken out, the ice cubes can continue to provide a low-temperature storage environment during the melting process. The low temperature of the melted ice water also has the function of providing a low-temperature storage environment.
[0037] By adopting the above method, the bottom of the reagent placed in the placement groove 41 has a good low-temperature preservation environment. In order to make the outer ring of the reagent also have a low-temperature environment, the present application also makes it possible to fix a ring frame 31 on the upper outer ring of the aluminum box 3. The ring frame 31 is in contact with the inner wall surface of the inner box 1. The bottom of the ring frame 31 is connected to the interior of the aluminum box 3 through the through-hole 32 opened on the outer ring surface of the aluminum box 3. The above-mentioned foam block 4 is placed above the aluminum box 3 on the inner side of the ring frame 31 for support, and the interior of the ring frame 31 is filled with a sponge block 311. The sponge block 311 is evenly filled in the interior of the ring frame 31, and the bottom of the sponge block 311 extends into the interior of the aluminum box 3 through the through-hole 32. When in use, the sponge block 311 will also be moistened with clean water and frozen into ice cubes, which can provide a low-temperature preservation environment for the outer ring of the reagent. After the ice cubes cool down, the cold water in the aluminum box 3 will moisten the sponge block 311, also providing a low-temperature preservation environment for the outer ring of the reagent.
[0038] The outer box 2 is fixed to the outer ring of the inner box 1, and the upper half of the inner box 1 is exposed to the outside of the outer box 2, and the top of the outer box 2 is connected to the outside. A sealing paper 23 is connected and fixed between the inner surface of the upper inner surface of the outer box 2 and the outer surface of the inner box 1. An easy-to-tear opening 231 is provided in the middle position of the surface of the sealing paper 23. The sealing paper 23 is used to seal the internal space of the outer box, and the sealing paper can be easily torn open through the easy-to-tear opening 231 to allow the internal structure of the outer box 2 to leak out. A heat sink 21 is fixed to the outer ring of the inner box 1 inside the outer box 2. The heat sink 21 is an aluminum plate, and the fins 22 are connected to the outer ring of the inner box 1. The aluminum plates are connected to form an integral structure, the inner surface of the heat sink 21 is in contact with the outer surface of the inner box 1, and fins 22 are evenly fixed on the surface of the heat sink 21, and the fins 22 are parallel to each other. When in use, when the test kit has been taken out and has not been used for a long time, and a long-term heat preservation effect needs to be maintained, the sealing paper 23 is torn open through the easy-tear opening 231, and crushed ice is placed in the space inside the outer box 2. The cool air is transferred to the heat sink 21 through the fins 22. When the heat sink 21 is an aluminum plate, it can directly provide a low-temperature heat preservation environment to the inside of the inner box 1.
[0039] And sponge strips 24 are filled between adjacent fins 22, and the bottom of the sponge strips 24 is placed at the bottom of the outer box 2. When the ice melts, cold water will penetrate the sponge strips 24 between the fins 22 due to capillary phenomenon, which is beneficial to cooling the aluminum plate and has a better low-temperature preservation effect on the inside of the inner box 1.
[0040] A cotton pad 11 is placed horizontally above the ring frame 31, and a 96-well plate 12 placed in the inner box 1 is supported above the cotton pad 11. A cotton pressing plate 53 is fixed to the bottom of the upper cover 5, and the cotton pressing plate 53 presses on the surface of the 96-well plate 12, so that the 96-well plate 12 is stored inside the inner box 1. The cooperation of the cotton pad 11 and the cotton pressing plate 53 can prevent the 96-well plate 12 from shaking when stored inside the inner box 1.
[0041] Bumps 51 are fixed at the four corners of the surface of the upper cover 5. The 96-well plate 12 can be placed inside the bumps 51 to be positioned and supported on the surface of the upper cover 5. During testing, after the 96-well plate 12 is taken out, it can be placed between the bumps 51 and supported on the surface of the upper cover 5, which makes it convenient to position and place the 96-well plate 12, thereby facilitating the testing work.
[0042] Example 2
[0043] After the aluminum plate is cooled by ice cubes, the low-temperature environment provided to the inside of the inner box 1 is limited. In order to achieve better low-temperature preservation of the inside of the inner box 1 when placed for a long time, the application also provides another real-time method. Under the premise of keeping other structures unchanged, the heat sink 21 no longer uses an aluminum plate, but is replaced by a semiconductor refrigeration plate. The fins 22 are fixed to the hot end surface of the semiconductor refrigeration plate, and the cold end of the semiconductor refrigeration plate is in contact with the outer surface of the inner box 1. When in use, ice cubes are used to dissipate heat to the fins 22. The fins 22 are located at the hot end of the semiconductor refrigeration plate, so that they are close to the cold end of the inner box 1 to provide a low-temperature environment, thereby realizing a low-temperature insulation environment inside the inner box 1. When the ice cubes melt, the cold water will penetrate the sponge strips 24 between the fins 22 due to capillary phenomenon, which is beneficial to the heat dissipation of the semiconductor refrigeration plate. The sponge strips 24 increase the contact area between the cold water and the air, which is beneficial to its evaporation and heat absorption, providing better heat dissipation effect, and avoiding the performance of the reagents placed inside the inner box 1 being affected by temperature rise.
[0044] Example 3
[0045] Since the sponge block 311 absorbs more water, its volume increases greatly when it is frozen into ice cubes, which can easily expand the ring frame 31. In order to solve this problem, the present application also provides a third embodiment, please refer to Figure 4 On the premise that other structures remain the same as those of Example 2, the above-mentioned sponge block 311 is replaced with a cotton rope 312. A plurality of cotton ropes 312 are evenly arranged, and the plurality of cotton ropes 312 extend vertically to the top of the ring frame 31. The bottom of the cotton rope 312 extends into the interior of the aluminum box 3 through the through-hole 32. In order to maintain the vertical state of the cotton rope 312, a partition 313 is vertically fixed inside the ring frame 31 between the cotton ropes 312. The cotton rope 312 is placed between the partitions 313 and is vertically constrained, which can reduce the amount of material used. The cotton rope 312 absorbs less water and its volume increases less when it is frozen into ice cubes. It can also have the same function as the sponge block 311. In order to prevent water from freezing into ice cubes and expanding to open the aluminum box 3, the water inside the aluminum box 3 cannot be overfilled.
[0046] When in use, test tubes containing lysate, proteinase K, RNA extraction reagent, buffer, etc. are placed in different placement slots 41, and other tools can also be placed in other placement slots 41. Then, the cotton pad 11 and the 96-well plate 12 are placed on top in sequence, and finally the upper cover 5 is covered so that the rubber ring 52 on the inner surface is pressed against the outer surface of the inner box 1, thereby preventing slipping. Finally, the inner box 1 and the outer box 2 are sealed as a whole and placed in a refrigerator for freezing. Since there is clean water inside the aluminum box 3, it will be frozen into ice cubes under the action of freezing, and some clean water will overflow into the sponge block 311 or The cotton rope 312 is also frozen into ice cubes. When the test kit is taken out of the refrigerator for genetic testing, the ice cubes frozen in the aluminum box 3 and the ring frame 31 can provide a low-temperature insulation environment. When the ice cubes melt, they will still have a low temperature. The melted cold water will also seep into the sponge block 311 or the cotton rope 312 due to capillary phenomenon, continuing to provide a low-temperature insulation environment for the bottom and outer ring of the reagent, avoiding the performance of the reagent being affected by temperature rise. When the test kit is taken out and has not been used for a long time and needs to maintain a long-term insulation effect, the plastic film can be torn off first and the test kit can be opened. The sealing paper 23 is torn open through the easy-tear opening 231, and crushed ice is put into the space inside the outer box 2. The cool air is transferred to the heat sink 21 through the fins 22. When the heat sink 21 is an aluminum plate, it can directly provide a low-temperature insulation environment for the inside of the inner box 1. When the heat sink 21 is a semiconductor refrigeration plate, ice is used to dissipate heat for the fins 22. The fins 22 are located at the hot end of the semiconductor refrigeration plate, so that the side facing the inner box 1 can provide a low-temperature environment, thereby realizing a low-temperature insulation environment for the inside of the inner box 1. When the ice melts, the cold water will infiltrate the sponge strips 24 between the fins 22 due to capillary phenomenon, which is beneficial to The aluminum plate or semiconductor refrigeration sheet is used for cooling or heat dissipation, and the sponge strip 24 increases the contact area between the cold water and the air, which is conducive to its evaporation and heat absorption, providing better cooling or heat dissipation effect, and avoiding the reagents placed inside the inner box 1 from affecting the performance due to temperature rise. When performing the test, first remove the upper cover 5, and then take out the 96-well plate 12 and the various reagents and tools placed in the placement slot 41 in turn, and cover the upper cover 5 again, place the 96-well plate 12 on the surface of the upper cover 5, and place it between the protrusions 51 for positioning, which is convenient for using tools and various reagents and using the 96-well plate 12 for genetic testing.
Claims
1. A glioma gene biomarker detection kit, comprising an inner box (1), an outer box (2), an aluminum box (3) and a foam block (4), wherein the upper sealed cover of the inner box (1) is provided with an upper cover (5), characterized in that: The outer box (2) is fixed to the outer ring of the inner box (1), the upper half of the inner box (1) is exposed outside the outer box (2), and the upper part of the outer box (2) is connected to the outside, and a sealing paper (23) is connected and fixed between the upper inner surface of the outer box (2) and the outer surface of the inner box (1), and an easy-to-tear notch (231) is provided in the middle of the surface of the sealing paper (23); A heat dissipation plate (21) is fixedly mounted on the outer ring of the inner box (1) inside the outer box (2), the inner surface of the heat dissipation plate (21) is in contact with the outer surface of the inner box (1), and fins (22) are evenly fixed on the surface of the heat dissipation plate (21), the fins (22) are parallel to each other, and sponge strips (24) are filled between adjacent fins (22), and the bottom of the sponge strips (24) is placed on the bottom of the outer box (2); An aluminum box (3) is placed at the bottom of the inner box (1), the interior of the aluminum box (3) is filled with clean water, a ring frame (31) is fixed on the outer ring of the upper part of the aluminum box (3), the ring frame (31) is in contact with the inner wall surface of the inner box (1), and the bottom of the ring frame (31) is connected to the interior of the aluminum box (3) through a through hole (32) opened on the outer ring surface of the aluminum box (3); The foam block (4) is placed above the aluminum box (3) inside the ring frame (31), and a plurality of placement grooves (41) are evenly opened on the surface of the foam block (4). A cotton pad (11) is placed horizontally above the ring frame (31), and a 96-well plate (12) placed in the inner box (1) is supported above the cotton pad (11). A cotton pressing plate (53) is fixed to the bottom of the upper cover (5), and the cotton pressing plate (53) presses against the surface of the 96-well plate (12).
2. The glioma gene biomarker detection kit according to claim 1, characterized in that: The heat dissipation plate (21) is an aluminum plate, and the fins (22) are connected to the aluminum plate to form an integral structure.
3. The glioma gene biomarker detection kit according to claim 1, characterized in that: The heat sink (21) is a semiconductor refrigeration sheet, the fins (22) are fixed to the hot end surface of the semiconductor refrigeration sheet, and the cold end of the semiconductor refrigeration sheet is in contact with the outer surface of the inner box (1).
4. The glioma gene biomarker detection kit according to claim 1, characterized in that: The interior of the ring frame (31) is filled with a water-absorbing structure, and the bottom of the water-absorbing structure extends into the interior of the aluminum box (3) through a through-hole (32).
5. The glioma gene biomarker detection kit according to claim 4, characterized in that: The water absorbing structure is a sponge block (311), and the sponge block (311) is evenly filled in the interior of the ring frame (31).
6. The glioma gene biomarker detection kit according to claim 4, characterized in that: The water absorbing structure is a cotton rope (312), a plurality of cotton ropes (312) are evenly arranged, and the plurality of cotton ropes (312) all extend vertically to the top inside the ring frame (31).
7. The glioma gene biomarker detection kit according to claim 6, characterized in that: A partition plate (313) is vertically fixed inside the ring frame (31) between the cotton ropes (312), and the cotton ropes (312) are placed between the partition plates (313) and are vertically bound.
8. The glioma gene biomarker detection kit according to claim 1, characterized in that: The four corners of the surface of the upper cover (5) are all fixed with protrusions (51), and the 96-well plate (12) can be placed inside the protrusions (51) to be positioned and supported on the surface of the upper cover (5).
9. The glioma gene biomarker detection kit according to claim 1, characterized in that: A rubber ring (52) is fixedly attached to the inner surface of the upper cover (5), and the rubber ring (52) is in contact with the outer surface of the inner box (1).