Tobacco polysaccharide extract and its application in buckwheat seed soaking agent formulation

Abstract

This invention discloses a tobacco polysaccharide extract and its application in a buckwheat seed soaking agent formulation. The extraction method includes: S1 using waste tobacco leaves as raw material, drying, pulverizing, and sieving to remove impurities before use; S2 extracting polysaccharides from the waste tobacco leaves using a compound enzyme-assisted extraction method, followed by centrifugation to obtain the supernatant, which is the crude polysaccharide solution; S3 decolorizing the crude polysaccharide solution using activated carbon decolorization, then deproteinizing using the Sevage deproteinization method, and purifying it using ion exchange chromatography and gel filtration chromatography to obtain high-purity tobacco polysaccharides. Finally, the polysaccharides are used as the main raw material to prepare a buckwheat seed soaking agent. This invention not only enables the high-value utilization of waste tobacco leaves but also improves the germination rate and yield of buckwheat seeds.

Description

Technical Field

[0001] This invention relates to a tobacco polysaccharide extract and its application in buckwheat seed soaking agent formulation, belonging to the interdisciplinary field of agricultural planting and biomaterials. Background Technology

[0002] Tartary buckwheat (Fagopyrum tataricum) belongs to the genus Fagopyrum in the family Polygonaceae. It is an annual crop with high nutritional and health value. However, the seed coat structure and air permeability of tartary buckwheat seeds, coupled with their high protein content, result in a significantly higher respiration rate during storage compared to conventional gramineous crops. This often leads to problems such as low seed germination rate and slow germination speed, limiting its cultivation and yield.

[0003] Soaking seeds is an effective seed treatment method that can significantly improve seed germination rate and promote seedling growth. However, the thick seed coat of tartary buckwheat makes it difficult for the seeds to absorb water and has poor air permeability, thus making conventional soaking treatments difficult to achieve the desired results. Currently reported methods for promoting tartary buckwheat seed germination include physical methods (laser method, graphene oxide method, rare earth method) and chemical methods (polyethylene glycol method), all of which have drawbacks to varying degrees, such as high cost, unstable effectiveness, and environmental pollution.

[0004] The production and processing of tobacco generate a large amount of waste tobacco leaves, including substandard leaves, processing scraps, and expired leaves, which no longer have any use or economic value. However, waste tobacco leaves are rich in polysaccharides, which, as natural polymers, have good water-holding capacity, biocompatibility, and biodegradability, and have broad application prospects in the agricultural field. However, there are currently no reports on technologies for extracting polysaccharides from waste tobacco leaves to prepare buckwheat seed soaking agents. Summary of the Invention

[0005] Based on the above, the present invention provides a tobacco polysaccharide extract and its application in the formulation of buckwheat seed soaking agent, which can not only realize the high-value utilization of waste tobacco leaves, but also improve the germination rate and yield of buckwheat seeds.

[0006] The technical solution of this invention is:

[0007] In a first aspect, the present invention provides a method for extracting polysaccharides from tobacco leaves, comprising:

[0008] S1 uses waste tobacco leaves as raw material, which are dried, crushed, and sieved to remove impurities before use;

[0009] S2 extracts polysaccharides from waste tobacco leaves using a compound enzyme-assisted extraction method. After extraction, the supernatant is obtained by centrifugation, which is the crude polysaccharide solution.

[0010] S3 decolorized the crude polysaccharide solution using activated carbon, followed by deproteinization using the Sevage deproteinization method, and finally purified it using ion exchange chromatography and gel filtration chromatography to obtain high-purity tobacco polysaccharide.

[0011] Preferably, in step S2, the process parameters of the compound enzyme-assisted extraction method are: enzymatic hydrolysis time 93-95 min, enzymatic hydrolysis temperature 45-55℃, liquid-to-solid ratio 50-55 mL / g, enzyme addition amount 1.2-1.8%, and the compound enzyme is composed of cellulase and pectinase mixed in a 2:1 ratio.

[0012] Preferably, in step S3, the process parameters of the activated carbon decolorization method are: activated carbon addition amount 1.2-1.5 g / 100mL, decolorization temperature 45-50℃, and decolorization time 75-85 min.

[0013] Preferably, in step S3, the process parameters of the Sevage deproteinization method are: deproteinization time 45-50 min, deproteinization times 3-5 times, Sevage solution: sugar solution ratio 0.9-1.0, and the Sevage is prepared by mixing chloroform and n-butanol in a 4:1 ratio.

[0014] Secondly, the present invention provides tobacco polysaccharides prepared by the above method.

[0015] Thirdly, the present invention provides the application of the above-mentioned tobacco polysaccharide in the preparation of buckwheat seed soaking agent products.

[0016] Fourthly, the present invention provides a buckwheat seed soaking agent, which is composed of tobacco polysaccharide, humic acid, tea saponin and deionized water, wherein the tobacco polysaccharide is 5~10 g / L, the humic acid is 0.5~1.0 g / L, the tea saponin is 0.2~0.9 g / L, and the balance is deionized water. The preparation method of the tobacco polysaccharide is as described above.

[0017] Preferably, after mixing the components, the pH is adjusted to 6.0-6.5 using citric acid or sodium bicarbonate solution, stirred evenly, and then filtered to remove bacteria, thus obtaining the buckwheat seed soaking agent.

[0018] The beneficial effects of this invention are:

[0019] 1. This invention extracts polysaccharides from waste tobacco leaves, solving the environmental pollution problem caused by the accumulation of waste tobacco leaves, while transforming low-value waste into high-value-added products and significantly reducing raw material costs. Specifically, through an optimized compound enzyme-assisted extraction method, the polysaccharide yield reaches as high as 26.81%; activated carbon decolorization achieves a decolorization rate of 95.76%, and the Sevage method for deproteinization increases the deproteinization rate by 48.97%. Combining ion exchange chromatography and gel filtration chromatography, the final polysaccharide purity is significantly improved (the refined polysaccharide has a galactose content of 39.34% and a glucuronic acid content of 5.05%).

[0020] 2. This invention utilizes a compound formula of tobacco polysaccharides, humic acid, and tea saponins. In particular, the water-holding capacity of tobacco polysaccharides increases the water absorption capacity of seeds, humic acid promotes root development, and tea saponins improve seed coat permeability. The three components work synergistically to overcome the air permeability barrier of buckwheat seed coat, thereby increasing the germination rate, germination potential, and vitality index of buckwheat seeds by 9.02%~21.42%, 11.86%, and 31.29%, respectively. Attached Figure Description

[0021] Figure 1 Infrared spectra of different types of polysaccharides; Note: crude polysaccharide (CP), decolorized polysaccharide (DP), deproteinized polysaccharide (DE), refined polysaccharide (RP);

[0022] Figure 2 Scanning electron microscopy (SEM) structures of different types of polysaccharides; Note: crude polysaccharides (a, b), decolorized polysaccharides (c, d), deproteinized polysaccharides (e, f), refined polysaccharides (g, h).

[0023] Figure 3 Polysaccharide yield using enzyme-assisted extraction method;

[0024] Figure 4 Response surface plots (a, b, c) and contour plots (d, e, f) showing the interactions between factors;

[0025] Figure 5 Polysaccharide yield by hot water extraction method;

[0026] Figure 6 Single-factor results of activated carbon decolorization method;

[0027] Figure 7 Response surface plots (a, b, c) and contour plots (d, e, f) showing the interactions between factors;

[0028] Figure 8 Decolorization rate of different types of macroporous resins;

[0029] Figure 9 Decolorization rate of tobacco polysaccharides under different decolorization methods and processes;

[0030] Figure 10 Response surface plots (a, b, c) and contour plots (d, e, f) showing the interactions between factors;

[0031] Figure 11 Sevage method deproteinization single-factor results;

[0032] Figure 12 Response surface plots (a, b, c) and contour plots (d, e, f) showing the interactions between factors;

[0033] Figure 13 Results of single-factor experiments on protein removal using the trichloroacetic acid-n-butanol method;

[0034] Figure 14 Response surface plots (a, b, c) and contour plots (d, e, f) showing the interactions between factors. Detailed Implementation

[0035] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0036] Example 1: Preparation of tobacco polysaccharide extract

[0037] S1 raw material source: Selected waste flue-cured tobacco, dried, crushed to 40 mesh, and sieved to remove impurities before use;

[0038] S2 polysaccharide preparation: Polysaccharides were extracted from waste tobacco leaves using a compound enzyme-assisted extraction method. After extraction, the supernatant was obtained by centrifugation, which is the crude polysaccharide solution. The process parameters of the compound enzyme-assisted extraction method are as follows: enzymatic hydrolysis time 94 min, enzymatic hydrolysis temperature 50℃, liquid-to-material ratio 54 ml / g, enzyme addition amount 1.57%, and the compound enzyme is composed of cellulase and pectinase mixed in a 2:1 ratio.

[0039] S3 Polysaccharide Purification: The crude polysaccharide solution was decolorized using activated carbon. The process parameters for activated carbon decolorization were: activated carbon addition 1.25 g / 100 mL, decolorization temperature 48℃, and decolorization time 79 min. Subsequently, deproteinization was performed using the Sevage deproteinization method. The process parameters for Sevage deproteinization were: deproteinization time 47 min, deproteinization times 4, and a Sevage solution:sugar solution ratio of 0.94. The Sevage solution was prepared by mixing chloroform and n-butanol in a 4:1 ratio. The decolorized and deproteinized solution was initially purified by ion exchange chromatography, followed by further purification by gel filtration chromatography to obtain high-purity polysaccharides.

[0040] For the crude polysaccharides, decolorized monosaccharides, deproteinized polysaccharides, and refined polysaccharides (purified polysaccharides) obtained from the above methods, the monosaccharide composition of the polysaccharides was determined by ion chromatography (IC). The results, as shown in Table 1, revealed that the waste tobacco leaf polysaccharides were composed of rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), xylose (Xyl), mannose (Man), galacturonic acid (Gal-UA), and glucuronic acid (Glc-UA) (Table 6). Among these, galactose (33.55–39.34%), glucose (18.07–24.85%), and arabinose (18.04–19.43%) were the main components.

[0041] Table 1. Monosaccharide composition of polysaccharides from different types of tobacco leaves

[0042]

[0043] Fourier transform infrared spectroscopy was used to analyze the functional groups in the polysaccharide molecules, and the results showed that the functional groups were located in the range of 4000–500 cm⁻¹. - ¹Six characteristic absorption peaks were found in the wavenumber range ( Figure 1 880cm - ¹ and 842cm - ¹Derived from α / β-glycosidic bond structure; 1085~1087cm - The ¹ position originates from the COC and C-OH structures in pyranose; 1417–1419 cm⁻¹ - ¹ and 1325cm - ¹Derived from C=O and CH structures; 1593~1599cm - ¹Derived from the C=O structure in carbonyl and amide compounds; 2928–2934 cm - The ¹ position originates from the CH structure in methyl (-CH3) and methylene (-CH2-).

[0044] Scanning electron microscopy analysis of the polysaccharide microstructure revealed that crude and decolorized polysaccharides had rough surfaces and formed coral-like aggregates; deproteinized polysaccharides consisted of irregularly stacked fine particles; and refined polysaccharides formed larger, irregularly aggregated structures with rough surfaces and dispersed aggregates, internally composed of polysaccharides of varying sizes connected together, containing pores. Figure 2 ).

[0045] The following is an optimization experiment for the above process parameters:

[0046] I. Selection and Optimization of Compound Enzyme-Assisted Extraction Method

[0047] 1000 g of tobacco waste was dried to constant weight and then pulverized through a 40-mesh sieve to obtain tobacco powder. A Box-Benhnken experimental design based on response surface methodology was used to compare the polysaccharide extraction rates of different enzyme extraction methods and hot water extraction methods, and to optimize and integrate the optimal process for polysaccharide extraction from waste tobacco leaves: tobacco powder was mixed with 85% ethanol and soaked for 24 hours to remove lipids and pigments; extraction was performed at 50℃ for 94 min at a liquid-to-solid ratio of 54 mL / g and with a combined enzyme (cellulase and pectinase) dosage of 1.57%; the crude polysaccharide supernatant was obtained by centrifugation. Specific results are as follows:

[0048] 1. Optimization of process conditions for enzyme extraction

[0049] A 4-factor, 5-level experimental design was adopted. Figure 3 The effects of key factors such as enzymatic hydrolysis time (A), enzymatic hydrolysis temperature (B), liquid-to-solid ratio (C), and enzyme dosage (D) on the extraction rate (Y) of tobacco polysaccharides were investigated. The results are as follows: Figure 1 As shown, the highest polysaccharide yield was achieved when the enzymatic hydrolysis time was 80 min, the enzymatic hydrolysis temperature was 50℃, the liquid-to-material ratio was 50 mL / g, and the enzyme addition was 1.5%.

[0050] Based on response surface analysis (Table 2, Figure 4 The quadratic multinomial regression model is obtained as follows: Y = -48.8217 + 0.465667A + 90175B + 0.89675C + 6.541667D + 0.000438AB - 0.00379AC + 0.14625AD + 0.01105BC - 0.005BD + 0.002CD - 0.00281A 2 -0.01448B 2 -0.01023C 2 -6.16333D 2The predicted optimal process parameters for enzyme-assisted extraction of tobacco polysaccharides were: hydrolysis time 94.15 min, hydrolysis temperature 49.54℃, liquid-to-solid ratio 53.81 ml / g, enzyme dosage 1.57%, and predicted value 26.67%. For ease of experimental operation, the parameters were adjusted to: hydrolysis time 94 min, hydrolysis temperature 50℃, liquid-to-solid ratio 54 ml / g, and enzyme dosage 1.57%. Verification experiments showed that under these conditions, the yield of polysaccharides from waste tobacco leaves reached 26.81%.

[0051] Table 2. Extraction rate of tobacco polysaccharides by enzyme-assisted extraction method

[0052]

[0053] 2. Optimization of process conditions for water extraction

[0054] A single-factor experimental design with 3 factors and 5 levels was used to investigate the effects of extraction time (A), temperature (B), and liquid-to-solid ratio (C) on the extraction rate (Y) of tobacco polysaccharides. Figure 5 ).

[0055] Based on response surface methodology (Table 3), the quadratic multinomial regression model for hot water extraction is obtained as follows: Y = -137.56750 + 1.34492A + 2.80908B + 0.440000C - 0.000775AB + 0.000950AC - 0.001000BC - 0.008004A 2 -0.019367B 2 -0.007517C 2 The predicted optimal process parameters for tobacco polysaccharide extraction by hot water extraction are: extraction time 93.19 min, extraction temperature 76.79℃, liquid-to-solid ratio 38.48 ml / g, and predicted polysaccharide yield 23.72%.

[0056] Table 3. Response surface methodology results of tobacco polysaccharide extraction rate using hot water extraction method

[0057]

[0058] II. Selection and Optimization of Activated Carbon Decolorization Method

[0059] Following the above, the crude polysaccharide solution from waste tobacco leaves was concentrated by rotary evaporation and then subjected to alcohol precipitation to obtain a flocculent precipitate. After centrifugation, the precipitate was freeze-dried to obtain crude polysaccharide. The crude polysaccharide was prepared into a 10 mg / mL polysaccharide solution, and a full-band scan was performed in the wavelength range of 200–700 nm. The maximum absorption wavelength was found to be 395 nm.

[0060] A Box-Benhnken experimental design based on response surface methodology was used to compare the polysaccharide yields of activated carbon decolorization and macroporous resin decolorization, and to optimize and integrate the optimal process for decolorizing polysaccharides from waste tobacco leaves: activated carbon addition of 1.25 g / 100 mL, decolorization temperature of 48℃, and decolorization time of 79 min.

[0061] 1. Optimization of process conditions for activated carbon decolorization

[0062] A three-factor, five-level experimental design was used to investigate the effects of decolorization time (A), temperature (B), and activated charcoal addition (C) on the retention rate of tobacco polysaccharides (Y). Figure 6 The results showed that the polysaccharide retention rate was highest when the decolorization time was 60 min, the temperature was 50℃, and the activated carbon addition was 1.0 g / 100 mL.

[0063] Based on response surface methodology, a quadratic multinomial regression model was constructed: Y = -52.0675 + 0.70512A + 1.69783B + 124.53666C - 0.003375AB - 0.281AC - 0.226BC - 0.001839A 2 -0.0107083B 2 -36.413333C 2 (Table 4, Figure 7 The model predicted the optimal decolorization process parameters for tobacco polysaccharides as follows: decolorization time 79.16 min, decolorization temperature 47.54°C, and char addition amount 1.25 g / 100ml, with a predicted value of 95.85%. For ease of experimental operation, the parameters were slightly adjusted to a decolorization time of 79 min, a decolorization temperature of 48°C, and a char addition amount of 1.25 g / 100ml. Validation experiments showed that the average decolorization rate of polysaccharides under these conditions reached 95.76%, which is close to the predicted value.

[0064] Table 4. Response surface methodology results of tobacco polysaccharide decolorization rate using activated carbon decolorization method

[0065]

[0066] 2. Optimization of process conditions for macroporous resin decolorization method

[0067] Decolorization was performed using macroporous resins of types D101, XAD-2, HPD600, and HPD100, respectively. It was found that type XAD-2 had the highest decolorization rate and polysaccharide retention rate. Figure 8 A 3-factor, 5-level experimental design was used, employing XAD-2 type macroporous resin, to investigate the effects of decolorization time (A), temperature (B), and macroporous resin dosage (C) on the decolorization (Y) of tobacco polysaccharides. Figure 9Based on response surface methodology, the quadratic multinomial regression model is obtained: Y = -170.563 + 35.098A + 7.389B + 19.5604C - 0.31AB - 1.38AC - 0.18025BC - 3.92166A 2 -0.06671B 2 -0.63697C 2 ( Figure 10 The predicted optimal decolorization process parameters for tobacco polysaccharides are: decolorization time 1.43 h, decolorization temperature 43.07°C, macroporous resin dosage 7.03 g / 100ml, with a predicted value of 84.87%.

[0068] III. Selection and Optimization of the Sevage Deproteinization Method

[0069] Waste tobacco crude polysaccharide powder was prepared into a 5 mg / mL polysaccharide solution. Sevage solution was prepared by mixing chloroform and n-butanol at a ratio of 4:1, and trichloroacetic acid-n-butanol reagent was prepared by mixing 10% trichloroacetic acid and n-butanol at a ratio of 1:10.

[0070] 1. Optimization of process conditions for Sevage deproteinization method

[0071] A three-factor, five-level experimental design was used to investigate the effects of deproteinization time (A), number of times (B), and amount of Sevage solution added (C) on the retention rate of tobacco polysaccharides (Y). Figure 11 The results showed that when the deproteinization time was 40 min, the number of times was 4, and the ratio of Sevage solution to polysaccharide solution was 1:1, the deproteinization rate and polysaccharide retention rate were optimal. Based on response surface methodology, a quadratic multinomial regression model was constructed: Y = -13.7321 + 1.817833A + 8.22625B + 6.805C + 0.03475AB - 0.24967AC + 2.96BC - 0.0181A 2 -1.6025B 2 -3.34667C 2 (Table 5, Figure 12 The predicted optimal deproteinization process parameters for tobacco polysaccharides were: deproteinization time 47.42 min, deproteinization cycles 3.83 times, Sevage solution:sugar solution ratio 0.936, and a predicted value of 48.84%. For ease of experimental operation, the parameters were adjusted to: deproteinization time 47 min, deproteinization cycles 4 times, and Sevage solution:sugar solution ratio 0.94. Verification experiments showed that the average deproteinization rate under these conditions reached 48.97%, which is close to the predicted value.

[0072] Table 5 - Results of the response surface methodology for reducing the protein content of tobacco polysaccharides using the Sevage method

[0073]

[0074] 2. Optimization of process conditions for protein removal using trichloroacetic acid-n-butanol method

[0075] A three-factor, five-level experimental design was used to investigate the effects of deproteinization time (A), number of times (B), and the amount of trichloroacetic acid-n-butanol solution added (C) on the retention rate of tobacco polysaccharides (Y). Figure 13 The results showed that when the deproteinization time was 30 min, the number of times was 3, and the ratio of trichloroacetic acid-n-butanol solution to polysaccharide solution was 1:2, the deproteinization rate and polysaccharide retention rate were better. Based on response surface methodology, a quadratic multinomial regression model was constructed: Y = -38.4754 + 2.4662A + 15.0973B + 23.6767C - 0.12493AB - 0.053517AC - 0.875738BC - 0.032991A 2 -1.6604B 2 -8.80163C 2 (Table 6, Figure 14 The optimal deproteinization process parameters for tobacco polysaccharides prepared by the trichloroacetic acid-n-butanol method are predicted to be: deproteinization time 28.09 min, deproteinization times 2.97, trichloroacetic acid-n-butanol:sugar solution ratio 0.925, and predicted value 35.12%.

[0076] Table 6 Results of response surface methodology for deproteinization rate of tobacco polysaccharides using trichloroacetic acid-n-butanol method

[0077]

[0078] Example 2: Preparation of Buckwheat Seed Soaking Agent

[0079] The tartary buckwheat seed soaking agent is composed of tobacco polysaccharide, humic acid, tea saponin, and deionized water. The tobacco polysaccharide concentration is 5-10 g / L, the humic acid concentration is 0.5-1.0 g / L, the tea saponin concentration is 0.2-0.9 g / L, and the remainder is deionized water. The preparation method of the tobacco polysaccharide is as described in Example 1. After mixing the components, the pH is adjusted to 6.0-6.5 using citric acid or sodium bicarbonate solution, stirred evenly, and then filtered through a 0.22 μm filter membrane for sterilization to obtain the tartary buckwheat seed soaking agent.

[0080] The following verifies the effectiveness of the buckwheat seed soaking agent:

[0081] Select plump, disease-free buckwheat seeds, rinse them thoroughly with clean water to remove surface impurities. Dilute the seed soaking agent 20 times with deionized water, then soak the pre-treated buckwheat seeds in the environmentally friendly buckwheat seed soaking agent prepared in this invention for 8-12 hours at a temperature of 20-25°C. The seed-to-solution ratio is 1:5-1:10 (w / v). After soaking, remove the seeds, drain off the surface soaking agent, and air dry them in a ventilated place until there is no visible water on the seed surface. Sow the dried buckwheat seeds according to conventional agronomic requirements.

[0082] Germination experiments were conducted on buckwheat seeds stored for three different years using two different concentrations of seed soaking agents, with a seed soaking agent without tobacco polysaccharides serving as a control. The results showed that seed soaking agents with polysaccharide concentrations of 0.25–0.50 g / L significantly promoted the germination rate of buckwheat seeds stored for different years (Table 7). Specifically, the seed soaking agent with a polysaccharide concentration of 0.50 g / L increased the germination rate of seeds stored for 3 years, 2 years, and 1 year by 21.42%, 11.84%, and 9.02%, respectively, compared to the control.

[0083] Table 7. Effects of soaking agent concentration on germination rate of buckwheat seeds stored for different years.

[0084]

[0085] Soaking seeds stored for one year before sowing revealed that soaking agents with polysaccharide concentrations of 0.25–0.50 g / L significantly improved seed germination potential, germination index, and vigor index (Table 8). Specifically, a 0.50 g / L polysaccharide soaking agent increased germination potential by 11.86%, germination index by 13.05%, and vigor index by 31.29%. Simultaneously, the soaking agent promoted the growth of buckwheat seedlings; a 0.50 g / L polysaccharide soaking agent increased seedling stem diameter by 4.41%, root length by 28.46%, fresh weight by 23.96%, and dry weight by 6.66%. Field trials confirmed that the soaking agent significantly increased yield, with a 0.50 g / L soaking agent increasing buckwheat yield by up to 10.44%.

[0086] Table 8 Effects of different concentrations of seed soaking agents on the growth and yield of buckwheat.

[0087]

[0088] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. Use of a tobacco polysaccharide in the preparation of a tartary buckwheat seed soaking agent product, wherein, The leaf polysaccharide extraction method includes the following steps: S1 uses waste tobacco leaves as raw material, which are dried, crushed, and sieved to remove impurities before use; S2 uses a compound enzyme-assisted extraction method to extract polysaccharides from waste tobacco leaves. After extraction, the supernatant is obtained by centrifugation, which is the crude polysaccharide solution. The process parameters of the compound enzyme-assisted extraction method are: enzymatic hydrolysis time 93-95 min, enzymatic hydrolysis temperature 45-55℃, liquid-to-material ratio 50-55 mL / g, enzyme addition amount 1.2-1.8%, and the compound enzyme is composed of cellulase and pectinase mixed in a 2:1 ratio. S3 decolorized the crude polysaccharide solution using activated carbon, followed by deproteinization using the Sevage deproteinization method, and finally purified it using ion exchange chromatography and gel filtration chromatography to obtain high-purity tobacco polysaccharide.

2. The use of the tobacco polysaccharide according to claim 1 in the preparation of a tartary buckwheat seed soaking agent product, characterized in that, In step S3, the process parameters for the activated carbon decolorization method are: activated carbon addition amount 1.2-1.5 g / 100mL, decolorization temperature 45-50℃, and decolorization time 75-85 min.

3. The use of the tobacco polysaccharide according to claim 1 in the preparation of a tartary buckwheat seed soaking agent product, characterized in that, In step S3, the process parameters of the Sevage deproteinization method are as follows: deproteinization time 45-50 min, deproteinization times 3-5 times, Sevage solution: sugar solution ratio 0.9-1.0, and the Sevage is prepared by mixing chloroform and n-butanol at a ratio of 4:

1.

4. A seed-dipping agent for tartary buckwheat, characterized by comprising: The product is composed of tobacco polysaccharide, humic acid, tea saponin, and deionized water, wherein the tobacco polysaccharide is 5-10 g / L, the humic acid is 0.5-1.0 g / L, the tea saponin is 0.2-0.9 g / L, and the balance is deionized water. The preparation method of the tobacco polysaccharide includes the following steps: S1 uses waste tobacco leaves as raw material, which are dried, crushed, and sieved to remove impurities before use; S2 uses a compound enzyme-assisted extraction method to extract polysaccharides from waste tobacco leaves. After extraction, the supernatant is obtained by centrifugation, which is the crude polysaccharide solution. The process parameters of the compound enzyme-assisted extraction method are: enzymatic hydrolysis time 93-95 min, enzymatic hydrolysis temperature 45-55℃, liquid-to-material ratio 50-55 mL / g, enzyme addition amount 1.2-1.8%, and the compound enzyme is composed of cellulase and pectinase mixed in a 2:1 ratio. S3 decolorized the crude polysaccharide solution using activated carbon, followed by deproteinization using the Sevage deproteinization method, and finally purified it using ion exchange chromatography and gel filtration chromatography to obtain high-purity tobacco polysaccharide.

5. The seed of F. tataricum of claim 4, wherein, In step S3, the process parameters for the activated carbon decolorization method are: activated carbon addition amount 1.2-1.5 g / 100mL, decolorization temperature 45-50℃, and decolorization time 75-85 min.

6. The seed of F. tataricum of claim 4, wherein, In step S3, the process parameters of the Sevage deproteinization method are as follows: deproteinization time 45-50 min, deproteinization times 3-5 times, Sevage solution: sugar solution ratio 0.9-1.0, and the Sevage is prepared by mixing chloroform and n-butanol at a ratio of 4:

1.

7. The buckwheat seed soaking agent according to claim 4, characterized in that, After mixing the components, adjust the pH to 6.0-6.5 using citric acid or sodium bicarbonate solution, stir evenly, and then filter to remove bacteria to obtain the buckwheat seed soaking agent.

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