Artificial aerial fog cultivation method for radix ranunculi ternati
By improving the Akadama-coconut coir composite matrix and aerosol culture medium, the problems of low matrix permeability and nutrient utilization in aerosol culture were solved, and the efficient growth and high survival rate of cat's claw were achieved.
Patent Information
- Application Number
- CN202511031949.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-25
- Publication Date
- 2025-10-10
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the existing aeroponic culture technology, conventional substrates are difficult to balance water retention and air permeability, which can easily lead to root hypoxia or lack of water. In addition, the metal ions in the nutrient solution precipitate and block the pores, affecting the survival rate and development of cat's claw.
A composite matrix of Akadama-coconut bran was used, and the hydrophilicity and air permeability of Akadama were improved by hydrogen peroxide treatment. Coconut bran was treated with calcium chloride to reduce the salt content. The metal ions in the aerosol culture medium were complexed with citric acid to prevent precipitation and clogging.
It significantly improves the survival rate and nutrient absorption efficiency of cat's claw, prolongs root development, prevents substrate particles from falling off and pores from clogging, and improves the effect of aeroponic culture.
Smart Images
Figure CN120753184A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of plant cultivation, and in particular to an artificial aeroponics cultivation method for cat's claw. Background Art
[0002] Cat's claw is a plant with important medicinal value. Its tuberous roots are rich in active ingredients such as saponins and polysaccharides. In traditional Chinese medicine, it is often used to treat lymphatic tuberculosis, assist in tumor treatment, and regulate the immune system. However, due to the high demand for its medicinal properties, wild cat's claw has been harvested in large quantities and without proper regulation, resulting in a sharp decline in its natural population and even local extinction. Furthermore, it takes 2-3 years for wild cat's claw to grow from seeds into medicinal tubers, and the seed germination rate is low. Therefore, artificial cultivation of cat's claw is needed to shorten its reproduction cycle and improve its reproduction efficiency.
[0003] Aerosol culture, as an advanced soilless cultivation technology, has significant advantages for the cultivation of cat's claw. Aerosol culture can directly expose the roots of cat's claw to an oxygen-rich atomized environment, improve metabolic efficiency, shorten the growth cycle, promote the expansion of tuberous roots, and thus increase yield. Conventional aerosol culture substrates such as rock wool and vermiculite have difficulty in balancing water retention and air permeability, which can easily lead to root hypoxia or insufficient water supply, affecting the survival rate of cat's claw. In addition, under long-term infiltration of nutrient solution, the particles of the culture matrix are easy to fall off, which may clog the aerosol nozzle or pores. The metal ions in the nutrient solution, such as iron ions and calcium ions, are also easy to precipitate in the culture matrix, clogging the pores and reducing the nutrient utilization rate, thereby affecting the development of cat's claw. Therefore, the present invention provides an artificial aerosol cultivation method for cat's claw to solve the problems of the above-mentioned prior art. Summary of the Invention
[0004] In view of the shortcomings of the prior art, the object of the present invention is to provide a method for artificial aeroponic cultivation of cat's claw.
[0005] A method for artificial aerosol cultivation of cat's claw comprises the following steps: The root pieces of cat's claw were cultured in a culture sponge until the root system was initially formed, and then transplanted into the aerosol system. A semi-enclosed box was selected as the culture tank of the aerosol system, in which a 5 cm thick red jade soil-coconut bran composite matrix was laid. A high-pressure spray pump, nozzle and timing device were installed, and the atomized particles were set at 20-50 microns. The high-pressure spray pump was then connected to a pool filled with aerosol culture solution. The temperature was controlled at 15-25 ° C, the air humidity was 60-70%, and the daily light time was 6-8 hours. In the early growth period after transplanting, the spray was sprayed once every 15-20 minutes, each spray was 5-10 seconds, and the spray volume was 50-100 mL / m 2 After the initial growth period of 15-25 days, spray for 5-8 seconds every 25-30 minutes during the growth period, with a spray volume of 100-200mL / m 2The cat's claw grass can be obtained by aeroponic culture for 150-180 days in the growth period.
[0006] Further, the preparation method of the akadama-coconut fiber composite substrate comprises the following steps: S1: 80-100 parts by mass of water glass is added to a reaction bottle, then 0.5-2 parts by mass of a surfactant is added, stirring at a speed of 60-80 r / min for 5-10 min, then 20-40 parts by mass of perlite powder is added, stirring at a speed of 500-600 r / min for 10-15 min, then the stirring speed is reduced to 200-250 r / min, and then the mixture is left to stand for 20-30 min to obtain a water glass-perlite cementing material; S2: 10-20 parts by mass of calcified coconut fiber is added to 50-60 parts by mass of deionized water, and dispersed at a speed of 1000-1100 r / min for 3-5 min, then 20-30 parts by mass of akadama is added, and stirred at a speed of 200-250 r / min for 10-15 min, 5-15 parts by mass of hydrogen peroxide solution is added during stirring, then 80-100 parts by mass of the water glass-perlite cementing material is added, and stirred at a speed of 300-350 r / min for 5-10 min, and then the mixture is poured into a mold to form circular particles with a diameter of 25 mm and 50 mm respectively, then the particles are left to stand at room temperature of 22-24°C for 24-25 h, and then dried at 50-60°C for 12-13 h to obtain the akadama-coconut fiber composite substrate.
[0007] Further, the preparation method of the calcified coconut fiber comprises the following steps: T1: Coconut fiber blocks are placed in a container, and water is added to completely immerse the coconut fiber blocks, and stirring is performed at a speed of 30-40 r / min for 24-25 h, then the liquid is filtered out, and the coconut fiber blocks are soaked and stirred with water again, and the soaking and stirring is repeated for 3 times to obtain pretreated coconut fiber; T2: 1-2 parts by mass of calcium chloride solid is dissolved in water to obtain a calcium chloride solution with a concentration of 0.1-0.2%, and 0.01% of Tween 80 is added, then the pretreated coconut fiber is immersed in the calcium chloride solution, and the solid-liquid ratio of the coconut fiber to the calcium chloride solution is 1 g: (5-6) mL, and the coconut fiber is soaked in a water bath at a temperature of 40-45°C for 1-2 h, then ultrasonic treatment is performed at a frequency of 40 kHz for 10-15 min, then the filtrate is removed by filtration, and the filter residue is washed with clean water for 2 times to obtain the calcified coconut fiber.
[0008] Further, the preparation method of the aeroponic culture solution comprises the following steps: 1-1.5 parts by mass of calcium nitrate solid is added to 100-110 parts by mass of deionized water, and stirred at 50-60 r / min for 5-10 minutes to obtain liquid A. Then, 0.1-0.5 parts by mass of potassium dihydrogen phosphate solid, 0.5-1 parts by mass of magnesium sulfate solid, and 0.1-0.2 parts by mass of potassium nitrate solid are added to 100-110 parts by mass of deionized water in sequence, and stirred to dissolve to obtain liquid B. Liquid B is then stirred at a speed of 100-110 r / min, and liquid A is poured into liquid B during the stirring process. Then, 0.5-1 parts by mass of citric acid and 0.15-0.25 parts by mass of EDTA-Fe are added, and the speed is maintained and stirred for 20-25 minutes to obtain an aerosol culture solution.
[0009] Furthermore, the surfactant is sodium lauryl sulfate.
[0010] Furthermore, the concentration of the hydrogen peroxide solution is 30-35%.
[0011] Furthermore, the volume ratio of the coconut husk blocks to water is 1:(10-12).
[0012] The present invention has the following advantages: 1. The present invention uses Akadama as a lightweight skeleton material and adds hydrogen peroxide when preparing the culture matrix. Hydrogen peroxide reacts with the metal elements in Akadama to generate hydroxyl radicals, which effectively degrade organic matter such as humic acid and oil that may be adsorbed on the surface of Akadama during mining, thereby eliminating the hydrophobic film formed by Akadama itself and improving the hydrophilicity of Akadama. At the same time, the reaction of hydrogen peroxide with Akadama during stirring can continuously eliminate the internal space of Akadama, thereby forming breathable gaps with charge adsorption. At the same time, the components of Akadama can Under the stimulation of water glass, a geopolymerization reaction occurs. Water glass provides hydroxyl groups to destroy the chemical bonds in the Akadama soil, further condensing to form a three-dimensional gel network. The gel network wraps the Akadama soil particles and fills the pores, significantly improving the physical strength of the compressive strength culture matrix while retaining open pores. During the aeroponic culture process, the Akadama soil is digested by hydrogen peroxide to form large pores that allow the atomized nutrient solution to penetrate quickly, and the coconut coir microporous adsorption water film is absorbed by the roots, ensuring the air permeability of the culture matrix while maintaining the retention of the aerosol nutrient solution, thereby improving the survival rate of cat's claw.
[0013] 2. The present invention utilizes calcium chloride and Tween 80 to soak coconut chaff to obtain calcified coconut chaff. The calcium ions in the calcium chloride undergo a replacement reaction with the salts adsorbed in the coconut chaff, significantly reducing the salt content in the coconut chaff and avoiding salt poisoning. Tween 80 acts as a nonionic surfactant to reduce the surface tension of the solution, promote the penetration of calcium ions into the interior of the coconut chaff fiber, and improve the desalination efficiency of the coconut chaff. At the same time, the calcified coconut chaff can slowly release calcium ions as a culture matrix, thereby preventing poor root development. At the same time, the fiber surface of the coconut chaff is negatively charged after calcification, and can be electrostatically adsorbed with perlite, thereby improving the bonding ability with the perlite. The stability of the coconut chaff fiber is enhanced after calcification, further reducing the risk of particle shedding in the culture matrix, preventing clogging of stomata and water channels, and improving the air permeability of the culture matrix, thereby preventing poor root development.
[0014] 3. The present invention adds citric acid to the prepared aerosol culture solution. During the aerosol culture process, when the aerosol culture solution is sprayed on the culture medium, the citric acid in the aerosol culture solution can form a soluble complex with the free cations in the culture matrix, thereby improving the absorption of the metal elements loaded on the culture matrix by the roots of cat's claw, thereby improving the absorption efficiency of cat's claw for nutrients. Moreover, the soluble complex will not remain in the micropores of the culture matrix in the form of precipitation, which can prevent the nutrients from forming precipitation to block the pores of the culture matrix, thereby improving the survival rate of cat's claw aerosol culture. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 The present invention is a flow chart of the artificial aerosol cultivation method of cat's claw. DETAILED DESCRIPTION
[0016] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.
[0017] Example 1:
[0018] A method for artificial aerosol cultivation of cat's claw grass, such as Figure 1 As shown, the following steps are included: The root pieces of cat's claw were cultured in a culture sponge until the root system was initially formed, and then transplanted into the aerosol system. A semi-enclosed box was selected as the culture tank of the aerosol system, and a 5 cm thick red jade soil-coconut bran composite matrix was laid in it. A high-pressure spray pump, a nozzle and a timing device were installed, and the atomized particles were set at 20 microns. The high-pressure spray pump was then connected to a pool filled with aerosol culture solution. The temperature was controlled between 15-25 ° C, the air humidity was between 60-70%, and the daily light time was 6 hours. In the early growth period after transplanting, the spray was sprayed once every 15 minutes, each spray was 5 seconds, and the spray volume was 50 mL / m 2After 15 days of initial growth, spray for 8 seconds every 25 minutes during the growth period, with a spray volume of 100 mL / m 2 , after culturing for 150 days during the growth period, you can get aerosol-cultured cat's claw.
[0019] The preparation method of the red jade soil-coconut husk composite matrix comprises the following steps: S1: 80 parts by mass of water glass were added to a reaction flask, followed by 0.5 parts by mass of a surfactant, sodium lauryl sulfate, and stirred at 60 rpm for 5 min. 20 parts by mass of perlite powder were then added, and stirred at 500 rpm for 10 min. The stirring speed was then reduced to 200 rpm, and the mixture was allowed to stand for 20 min to obtain a water glass-perlite gelling material. S2: 10 parts by mass of calcified coconut pith were added to 50 parts by mass of deionized water and dispersed at a speed of 1000 r / min for 3 minutes. Subsequently, 20 parts by mass of red jade were added and stirred at a speed of 200 r / min for 10 minutes. During the stirring, 5 parts by mass of a 30% hydrogen peroxide solution were added. Subsequently, 80 parts by mass of a water glass-perlite gelling material were added and stirred at a speed of 300 r / min for 5 minutes. The mixture was added to a mold and pressed into round particles with diameters of 25 mm and 50 mm respectively. The mixture was then allowed to stand at room temperature of 22°C for 24 hours and then dried at 50°C for 12 hours to obtain a red jade-coconut pith composite matrix.
[0020] The preparation method of calcified coconut husk comprises the following steps: T1: Place coconut husk blocks in a container and add water until they are completely submerged. The volume ratio of coconut husk blocks to water is 1:10. Stir at a speed of 30 r / min for 24 hours. Then filter out the liquid, add water again to soak and stir. Repeat this process three times to obtain pretreated coconut husks. T2: Dissolve 1-2 parts by mass of calcium chloride solid in water to a concentration of 0.1% to obtain a calcium chloride solution, then add 0.01% Tween 80, immerse the pretreated coconut husk in the calcium chloride solution, and the material-liquid ratio of coconut husk to calcium chloride solution is 1g:5mL. Heat the water bath to 40°C and soak for 1 hour, then ultrasonically treat at 40kHz for 10 minutes, then filter and remove the filtrate, and rinse the filter residue twice with clean water to obtain calcified coconut husk.
[0021] The method for preparing the aerosol culture solution comprises the following steps: 1-1.5 parts by mass of calcium nitrate solid was added to 100 parts by mass of deionized water, and stirred at 50 r / min for 5 minutes to obtain liquid A. Then, 0.1 parts by mass of potassium dihydrogen phosphate solid, 0.5 parts by mass of magnesium sulfate solid, and 0.1 parts by mass of potassium nitrate solid were added to 100 parts by mass of deionized water in sequence, and stirred to dissolve to obtain liquid B. Liquid B was then stirred at a speed of 100 r / min, and liquid A was poured into liquid B during the stirring process. Then, 0.5 parts by mass of citric acid and 0.15 parts by mass of EDTA-Fe were added, and the speed was maintained and stirred for 20 minutes to obtain an aerosol culture medium.
[0022] Example 2:
[0023] A method for artificial aerosol cultivation of cat's claw grass, such as Figure 1 As shown, the following steps are included: The root pieces of cat's claw were cultured in a culture sponge until the root system was initially formed, and then transplanted into the aerosol system. A semi-enclosed box was selected as the culture tank of the aerosol system, in which a 5 cm thick red jade soil-coconut bran composite matrix was laid. A high-pressure spray pump, a nozzle and a timing device were installed. The atomized particles were set at 50 microns. The high-pressure spray pump was then connected to a pool filled with aerosol culture solution. The temperature was controlled between 15-25 ° C, the air humidity was between 60-70%, and the daily light time was 8 hours. In the early growth period after transplanting, the spray was sprayed once every 20 minutes, each spray was 10 seconds, and the spray volume was 100 mL / m 2 After 25 days of initial growth, spray for 8 seconds every 30 minutes during the growth period, with a spray volume of 200 mL / m 2 , after culturing for 180 days during the growth period, you can get aerosol-cultured cat's claw.
[0024] The preparation method of the red jade soil-coconut husk composite matrix comprises the following steps: S1: 80 parts by mass of water glass were added to a reaction flask, followed by the addition of 0.5 parts by mass of a surfactant, sodium lauryl sulfate, and stirred at 80 rpm for 10 min. Then, 20 parts by mass of perlite powder were added, and stirred at 600 rpm for 15 min. The stirring speed was then reduced to 250 rpm, and the mixture was allowed to stand for 30 min to obtain a water glass-perlite gelling material. S2: 10 parts by mass of calcified coconut pith were added to 50 parts by mass of deionized water and dispersed at a speed of 1100 r / min for 3-5 minutes, followed by adding 20 parts by mass of red jade and stirring at a speed of 250 r / min for 15 minutes. During stirring, 5 parts by mass of a 35% hydrogen peroxide solution were added, followed by adding 80 parts by mass of a water glass-perlite gelling material, stirring at a speed of 350 r / min for 10 minutes, and then added to a mold and pressed into round particles with diameters of 25 mm and 50 mm respectively. The mixture was then allowed to stand at room temperature of 24°C for 25 hours and then dried at 60°C for 13 hours to obtain a red jade-coconut pith composite matrix.
[0025] The preparation method of calcified coconut husk comprises the following steps: T1: Place coconut husk blocks in a container and add water until they are completely submerged. The volume ratio of coconut husk blocks to water is 1:10. Stir at a speed of 40 r / min for 25 h. Then filter out the liquid, add water again to soak and stir, and repeat this three times to obtain pretreated coconut husks. T2: Dissolve 1 part by mass of calcium chloride solid in water to a concentration of 0.1% to obtain a calcium chloride solution, then add 0.01% Tween 80, immerse the pretreated coconut husk in the calcium chloride solution, and the solid-liquid ratio of coconut husk to calcium chloride solution is 1g:5mL. Heat the water bath to 45°C and soak for 2h, then ultrasonically treat at 40kHz for 15min, then filter and remove the filtrate, and rinse the filter residue twice with clean water to obtain calcified coconut husk.
[0026] The method for preparing the aerosol culture solution comprises the following steps: 1 part by mass of calcium nitrate solid was added to 100 parts by mass of deionized water, and stirred at 60 r / min for 10 minutes to obtain liquid A. Then, 0.1 parts by mass of potassium dihydrogen phosphate solid, 0.5 parts by mass of magnesium sulfate solid, and 0.1 parts by mass of potassium nitrate solid were added to 100 parts by mass of deionized water in sequence, and stirred to dissolve to obtain liquid B. Liquid B was then stirred at a speed of 100 r / min, and liquid A was poured into liquid B during the stirring process. Then, 0.5 parts by mass of citric acid and 0.15 parts by mass of EDTA-Fe were added, and the speed was maintained and stirred for 25 minutes to obtain an aerosol culture medium.
[0027] Example 3:
[0028] A method for artificial aerosol cultivation of cat's claw grass, such as Figure 1 As shown, the following steps are included: The root pieces of cat's claw were cultured in a culture sponge until the root system was initially formed, and then transplanted into the aerosol system. A semi-enclosed box was selected as the culture tank of the aerosol system, in which a 5 cm thick red jade soil-coconut bran composite matrix was laid. A high-pressure spray pump, nozzle and timing device were installed, and the atomized particles were set at 20 microns. The high-pressure spray pump was then connected to a pool filled with aerosol culture solution. The temperature was controlled between 15-25 ° C, the air humidity was between 60-70%, and the daily light time was 6 hours. In the early growth period after transplanting, the spray was sprayed once every 15 minutes, each spray was 5 seconds, and the spray volume was 50 mL / m 2 After 15 days of initial growth, spray for 5 seconds every 25 minutes during the growth period, with a spray volume of 100 mL / m 2 , after culturing for 150 days during the growth period, you can get aerosol-cultured cat's claw.
[0029] The preparation method of the red jade soil-coconut husk composite matrix comprises the following steps: S1: 100 parts by mass of water glass were added to a reaction flask, followed by 2 parts by mass of a surfactant, sodium lauryl sulfate, and stirred at 60 rpm for 5 min. 40 parts by mass of perlite powder were then added, and stirred at 500 rpm for 10 min. The stirring speed was then reduced to 200 rpm, and the mixture was allowed to stand for 20 min to obtain a water glass-perlite gelling material. S2: 20 parts by mass of calcified coconut pith were added to 60 parts by mass of deionized water and dispersed at a speed of 1000 r / min for 3 minutes. Subsequently, 30 parts by mass of red jade were added and stirred at a speed of 200 r / min for 10 minutes. During the stirring, 15 parts by mass of a 30% hydrogen peroxide solution were added. Subsequently, 100 parts by mass of a water glass-perlite gelling material were added and stirred at a speed of 300 r / min for 5 minutes. The mixture was added to a mold and pressed into round particles with diameters of 25 mm and 50 mm respectively. The mixture was then allowed to stand at room temperature of 22°C for 24 hours and then dried at 50°C for 12 hours to obtain a red jade-coconut pith composite matrix.
[0030] The preparation method of calcified coconut husk comprises the following steps: T1: Place coconut husk blocks in a container and add water until they are completely submerged. The volume ratio of coconut husk blocks to water is 1:12. Stir at a speed of 30 r / min for 24 hours. Then filter out the liquid, add water again to soak and stir. Repeat this process three times to obtain pretreated coconut husks. T2: Dissolve 2 parts by mass of calcium chloride solid in water to a calcium chloride concentration of 0.2% to obtain a calcium chloride solution, then add 0.01% Tween 80, immerse the pretreated coconut husk in the calcium chloride solution, and the material-liquid ratio of coconut husk to calcium chloride solution is 1 g:6 mL. Heat the water bath to 40°C and soak for 1 hour, then ultrasonically treat at 40 kHz for 10 minutes, then filter and remove the filtrate, and rinse the filter residue twice with clean water to obtain calcified coconut husk.
[0031] The method for preparing the aerosol culture solution comprises the following steps: 1.5 parts by mass of calcium nitrate solid was added to 110 parts by mass of deionized water, and stirred at 50 r / min for 5 minutes to obtain liquid A. Then, 0.5 parts by mass of potassium dihydrogen phosphate solid, 1 part by mass of magnesium sulfate solid, and 0.2 parts by mass of potassium nitrate solid were added to 110 parts by mass of deionized water in sequence, and stirred to dissolve to obtain liquid B. Subsequently, liquid B was stirred at a speed of 100 r / min, and liquid A was poured into liquid B during the stirring process. Then, 1 part by mass of citric acid and 0.25 parts by mass of EDTA-Fe were added, and the speed was maintained and stirred for 20 minutes to obtain an aerosol culture medium.
[0032] Comparative Example 1: Compared with Example 1, Comparative Example 1 is different in that hydrogen peroxide is not added in step S2, and the other steps remain unchanged, and is recorded as Comparative Example 1.
[0033] Comparative Example 2: Compared with Example 1, Comparative Example 2 is different in that calcified coconut husk is not added in step S2, and the other steps remain unchanged, which is recorded as Comparative Example 2.
[0034] Comparative Example 3: Compared with Example 1, Comparative Example 3 is different in that step T2 is not performed, pretreated coconut husk is used instead of calcified coconut husk in step S2, and the other steps remain unchanged, which is recorded as Comparative Example 3.
[0035] Comparative Example 4: Compared with Example 1, Comparative Example 4 is different in that citric acid is not added in the preparation of the aerosol culture solution, and the other steps remain unchanged, and is recorded as Comparative Example 4.
[0036] Comparative Example 5: Compared with Example 1, Comparative Example 5 is to replace the aerosol culture solution with a commercially available aerosol culture solution, and to culture Ranunculaceae in the same culture method.
[0037] Comparative Example 6: Compared with Example 1, Comparative Example 6 replaces the culture substrate with a commercially available culture substrate and cultivates Cat's Claw without changing the culture method.
[0038] Root pieces of the same mass were divided into 9 groups, 10 in each group, and cultured for 180 days using Examples 1-3 and Comparative Examples 1-6. The top 5 cat's claw plants with better growth were selected, and the average length, average diameter, and average fresh weight of the selected 5 cat's claw root pieces were measured and calculated, as shown in Table 1.
[0039] Root pieces of the same mass were divided into 9 groups, 10 in each group, and root culture was performed using the methods of Examples 1-3 and Comparative Examples 1-6. The survival rate of Cat's Claw was observed after 30 days of culture, as shown in Table 2.
[0040] Table 1 Average length (cm) Average diameter (mm) Average fresh weight per plant (g) Example 1 2.8 7.6 4.8 Example 2 2.7 7.8 4.7 Example 3 2.8 7.7 4.8 Comparative Example 1 1.1 6.4 4.2 Comparative Example 2 0.8 5.9 3.8 Comparative Example 3 1.2 6.5 4.2 Comparative Example 4 1.3 6.2 4.1 Comparative Example 5 1.5 6.4 4.3 Comparative Example 6 1.4 6.6 4.4 Table 2 Survival rate (%) Example 1 90 Example 2 100 Example 3 90 Comparative Example 1 70 Comparative Example 2 60 Comparative Example 3 70 Comparative Example 4 70 Comparative Example 5 80 Comparative Example 6 80 It can be seen from Table 1 and Table 2 that the average length, diameter and fresh weight of each plant of Examples 1-3 are better than those of the control example, and the survival rate is also higher. It can be seen that the culture matrix and aerosol culture solution prepared by the present invention have the effect of improving the survival rate of cat's claw compared with commercially available products, and can enable cat's claw to absorb more nutrients, thereby making the root tubers of cat's claw higher in length, diameter and fresh weight after culture.
[0041] It should be understood that those skilled in the art may make improvements or modifications based on the above description, and all such improvements and modifications shall fall within the scope of protection of the appended claims. Any portion of this specification not described in detail is prior art known to those skilled in the art.
Claims
1. A method for artificial aeroponic cultivation of cat's claw, characterized in that: The steps include: The root pieces of cat's claw were cultured in a culture sponge until the root system was initially formed, and then transplanted into the aerosol system. A semi-enclosed box was selected as the culture tank of the aerosol system, in which a 5 cm thick red jade soil-coconut bran composite matrix was laid. A high-pressure spray pump, nozzle and timing device were installed, and the atomized particles were set at 20-50 microns. The high-pressure spray pump was then connected to a pool filled with aerosol culture solution. The temperature was controlled at 15-25 ° C, the air humidity was 60-70%, and the daily light time was 6-8 hours. In the early growth period after transplanting, the spray was sprayed once every 15-20 minutes, each spray was 5-10 seconds, and the spray volume was 50-100 mL / m 2 After the initial growth period of 15-25 days, spray for 5-8 seconds every 25-30 minutes during the growth period, with a spray volume of 100-200mL / m 2 , after culturing for 150-180 days during the growth period, you can get aerosol-cultured cat's claw.
2. The artificial aeroponic cultivation method of cat's claw according to claim 1, characterized in that: The preparation method of the red jade soil-coconut husk composite matrix comprises the following steps: S1: Add 80-100 parts by mass of water glass to a reaction bottle, then add 0.5-2 parts by mass of a surfactant, stir at 60-80 r / min for 5-10 minutes, then add 20-40 parts by mass of perlite powder, stir at 500-600 r / min for 10-15 minutes, then reduce the stirring speed to 200-250 r / min, add 5-15 parts by mass of hydrogen peroxide solution during stirring, and then let it stand for 20-30 minutes to obtain a water glass-perlite gelling material; S2: 10-20 parts by mass of calcified coconut pith are added to 50-60 parts by mass of deionized water and dispersed at a speed of 1000-1100 r / min for 3-5 minutes, followed by adding 20-30 parts by mass of red jade and stirring at a speed of 200-250 r / min for 10-15 minutes, followed by adding 80-100 parts by mass of water glass-perlite gelling material, stirring at a speed of 300-350 r / min for 5-10 minutes, and adding it to a mold and pressing it into round particles with diameters of 25 mm and 50 mm respectively, and then standing at room temperature of 22-24°C for 24-25 hours, and then drying at 50-60°C for 12-13 hours to obtain a red jade-coconut pith composite matrix.
3. The artificial aeroponic cultivation method of cat's claw according to claim 2, characterized in that: The preparation method of calcified coconut husk comprises the following steps: T1: Place coconut husk blocks in a container, add water until they are completely submerged, and stir at a speed of 30-40 r / min for 24-25 hours. Then filter out the liquid, add water again to soak and stir, and repeat this three times to obtain pretreated coconut husks. T2: Dissolve 1-2 parts by mass of calcium chloride solid in water to a calcium chloride concentration of 0.1-0.2% to obtain a calcium chloride solution, then add 0.01% Tween 80, immerse the pretreated coconut husk in the calcium chloride solution, and the material-liquid ratio of coconut husk to calcium chloride solution is 1g: (5-6)mL. Heat the water bath to 40-45℃ and soak for 1-2h, then ultrasonically treat at 40kHz for 10-15min, then filter and remove the filtrate, and rinse the filter residue twice with clean water to obtain calcified coconut husk.
4. The artificial aeroponic cultivation method of cat's claw according to claim 1, characterized in that: The preparation method of the aerosol culture solution comprises the following steps: 1-1.5 parts by mass of calcium nitrate solid is added to 100-110 parts by mass of deionized water, and stirred at 50-60 r / min for 5-10 minutes to obtain liquid A. Then, 0.1-0.5 parts by mass of potassium dihydrogen phosphate solid, 0.5-1 parts by mass of magnesium sulfate solid, and 0.1-0.2 parts by mass of potassium nitrate solid are added to 100-110 parts by mass of deionized water in sequence, and stirred to dissolve to obtain liquid B. Liquid B is then stirred at a speed of 100-110 r / min, and liquid A is poured into liquid B during the stirring process. Then, 0.5-1 parts by mass of citric acid and 0.15-0.25 parts by mass of EDTA-Fe are added, and the speed is maintained and stirred for 20-25 minutes to obtain an aerosol culture solution.
5. The artificial aeroponic cultivation method of cat's claw according to claim 2, characterized in that: The surfactant is sodium lauryl sulfate.
6. The artificial aeroponic cultivation method of cat's claw according to claim 2, characterized in that: The concentration of hydrogen peroxide solution is 30-35%.
7. The artificial aeroponic cultivation method of cat's claw according to claim 3, characterized in that: The volume ratio of coconut coir to water is 1:(10-12).