Scalp care composition and preparation method thereof

Abstract

The invention relates to a scalp care composition and a preparation method thereof, and belongs to the technical field of biomedicine and cosmetics. The composition is prepared from the following components in percentage by mass: stem cell source exosome, epithelial cell source exosome, resveratrol, glutathione, sodium adenosine triphosphate, ceramide, a plant polypeptide extract, a plant tannin compound, sodium hyaluronate, nicotinamide, phosphatidylserine and the like, and a polygonum multiflorum extract can be selectively added. The whole system is subjected to nano encapsulation and collaborative optimization design, and has the composite conditioning performance of adjusting stratum corneum moisture, maintaining the barrier function, relieving inflammatory response and improving the scalp microenvironment. The preparation method is simple in process and has a wide application prospect.

Description

Technical Field

[0001] The present invention relates to the fields of cosmetics and biomedical preparations, and in particular to a scalp care composition and a preparation method thereof. Background Art

[0002] As a vital component of human skin, the scalp not only protects the head, regulates moisture, and secretes sebum, but also directly impacts hair follicle health and hair growth quality. With the accelerating pace of life and increasing environmental pollution, scalp problems are becoming increasingly common, such as unbalanced oil secretion, increased dandruff, a disrupted microbiome, inflammation, and hair loss, severely impacting both appearance and mental well-being. In the long-term development of chronic scalp problems, disrupted scalp microbiome and a deteriorating hair follicle microenvironment are becoming key factors in triggering hair loss.

[0003] It is currently known that various scalp discomfort conditions can cause chronic mild inflammation around the hair follicles, leading to apoptosis of hair papilla cells, atrophy of hair follicles, or premature entry into the resting phase, resulting in the phenomenon of "non-scarring hair loss", including seborrheic alopecia, stress-induced alopecia, and seasonal hair loss. At the same time, most hair loss products on the market focus on a single mechanism, such as simple vasodilation, simple moisturizing, or anti-inflammatory effects. They have problems such as single ingredients, low absorption efficiency, strong irritation, and unstable effects with long-term use. It is difficult to comprehensively intervene in multiple aspects such as scalp barrier repair, microcirculation regulation, and hair follicle cycle maintenance. In addition, although some natural active ingredients such as exosomes, antioxidants, biomimetic lipids, and traditional Chinese medicine extracts have a good theoretical basis, they are often difficult to effectively cooperate due to their poor stability, compatibility, and transfer efficiency in the system, which restricts their practical application in complex scalp conditioning products.

[0004] Therefore, there is an urgent need to develop a scalp care composition with a multi-component synergistic design that has barrier repair, microecological regulation and hair follicle environment support functions, which can more systematically address scalp discomfort and hair loss problems caused by multiple factors and improve the comprehensive performance and safety of care products. Summary of the Invention

[0005] The present invention aims to provide a scalp care composition and a method for preparing the same. The composition aims to improve the scalp barrier function, enhance moisture retention, and regulate the scalp microenvironment by constructing a multi-component synergistic system, thereby overcoming the problems of existing products such as single ingredients, short-lasting effects, strong irritation, or lack of deep repair capabilities.

[0006] To achieve the above object, the present invention proposes the following technical solution: a scalp care composition, comprising the following components by mass percentage: 1-8% stem cell-derived exosomes, 0.5-4% epithelial cell-derived exosomes; Resveratrol 0.01-0.5%, glutathione 0.01-0.3%, sodium adenosine triphosphate 0.005-0.1%, ceramide 0.05-0.5%, plant peptide extract 0.1-3%, plant tannin complex 0.01-0.3%, sodium hyaluronate 0.01-0.5%, niacinamide 0.05-1%; Sodium polyglutamate 0.01-0.3%, phosphatidylserine 0.01-0.2%, carnosine 0.005-0.1%, hydrolyzed oat beta-glucan 0.01-0.2%, squalane 0.1-1%, arginine lactate 0.01-0.3%, lipoic acid 0.001-0.05%; The carrier matrix balance is up to 100%; The carrier matrix includes one or more combinations of water, glycerol, butylene glycol, propylene glycol, Tween 80, acetylated sodium hyaluronate and lecithin, and the pH value of the overall system is controlled at 5.0-6.5, the osmotic pressure is 260-310 mOsm / kg, and the shear viscosity is controlled at 30-150 mPa·s.

[0007] In a preferred technical solution, the following mass percentage ratios are used: 3% stem cell-derived exosomes, 2% epithelial cell-derived exosomes, 0.1% resveratrol, 0.1% glutathione, 0.02% sodium adenosine triphosphate, 0.3% ceramide, 1.2% plant polypeptide extract, 0.1% plant tannin complex, 0.1% sodium hyaluronate, 0.3% niacinamide, 0.1% sodium polyglutamate, 0.05% phosphatidylserine, 0.02% carnosine, 0.05% hydrolyzed oat β-glucan, 0.3% squalane, 0.05% arginine lactate, 0.005% lipoic acid, and the remainder of the carrier matrix to 100%.

[0008] In a preferred technical solution, the stem cell-derived exosomes are selected from human adipose stem cell exosomes, human umbilical cord mesenchymal stem cell exosomes or dental pulp stem cell exosomes, with a particle size of 30 to 150 nm, and the exosome protein content is 20 to 100 μg / mL after detection by CD63, CD81 and Tsg101 antibodies.

[0009] In a preferred technical solution, the epithelial cell-derived exosomes are derived from human hair follicle epithelial cells, keratinocytes or their precursor cells, and are extracted by density gradient centrifugation and 0.1 μm ultrafiltration membrane, and the Zeta potential is controlled at −15 mV to −30 mV.

[0010] In a preferred technical solution, the resveratrol and glutathione are compounded in a 1:1 ratio, dissolved in an ethanol phase containing Tween 80, and then encapsulated by hydrogenated lecithin and cholesterol in a mass ratio of 2:1 to 4:1 to form inclusions with a particle size of 80 to 150 nm, and dispersed in an aqueous phase to form a stable emulsion system.

[0011] In a preferred technical solution, one or more of the following optional components is further included: asiaticoside 0.005-0.05%, glycyrrhizic acid dipotassium 0.005-0.1%, Schizochytrium fermentum 0.01-0.2%, ferulic acid 0.01-0.1%, oat peptide 0.01-0.2%, whey protein hydrolysate 0.01-0.2%, and radix polygoni multiflori extract 0.01-0.2%.

[0012] The radix polygoni multiflori extract is a water-soluble or alcohol-soluble component extracted from the dried tuber of radix polygoni multiflori, which is rich in bioactive compounds such as emodin, chrysophanol, and stilbene glycoside. Such components have a good application basis in regulating the scalp microenvironment and maintaining the dynamic balance of hair follicle cycles. Combined with the exosome conditioning factor, the biomimetic lipid structure, and the anti-oxidation and anti-inflammatory components in the present application, a synergistic complementary system can be formed to enhance the stability and functionality of the scalp environment from multiple aspects.

[0013] The present application also provides a preparation method of the scalp care composition, comprising the following steps: S1, respectively extract stem cell-derived exosomes and epithelial cell-derived exosomes, concentrate by 0.1 mu m ultrafiltration, and freeze at -80 DEG C for standby; S2, slowly dissolve the exosomes in a system containing glycerol and HEPES buffer, and adjust the temperature to 4-8 DEG C; S3, compound resveratrol and glutathione in a certain proportion, then add into ethanol phase, dissolve and slowly emulsify into phospholipid-cholesterol organic solution, remove the solvent by rotary evaporation to form lipid film, then hydrate to form complex inclusion body emulsion; S4, add the exosome system obtained in step S2 into the emulsion obtained in step S3, disperse by ultrasonic for 10 minutes, and perform high-pressure homogenization twice; S5, add ceramide, nicotinamide, squalane, plant polypeptide, sodium polyglutamate, conditioning agent, humectant, and radix polygoni multiflori extract, stir uniformly, adjust the pH to 5.5-6.5, sterilize by using 0.22 mu m filter membrane, and fill to obtain the finished product.

[0014] In a preferred technical solution, the cells used before extracting exosomes in step S1 are cultured for three generations or less in an animal serum-free environment, and the endotoxin of the extracted exosomes is less than or equal to 0.5 EU / mL.

[0015] In a preferred technical solution, the phospholipid emulsion is hydrogenated lecithin and cholesterol in a ratio of 2:1-4:1, assisted by Tween 80 and PEG-40 hydrogenated castor oil to form a mixed emulsion phase, and nano-sized emulsion particles are prepared by using probe ultrasonic for 60-120 seconds.

[0016] In a preferred technical solution, the feature is that the finished product is stored at 2-10° C. away from light and is packaged in an aluminum-plastic hose filled with inert gas or a multi-layer emulsion bottle with an air barrier valve.

[0017] The beneficial effects of the present invention are: 1. The scalp care composition provided by this invention offers significant technical synergy advantages in its composition and structural design. The combined application of stem cell-derived exosomes and epithelial cell-derived exosomes can achieve complementary effects in skin barrier repair and hair follicle microenvironment regulation. Stem cell exosomes primarily activate dermal cells and deliver growth factors, while epithelial cell exosomes help maintain the integrity of intercellular connections in the stratum corneum. Together, these two components can enhance skin barrier stability and improve tolerance to external stimuli.

[0018] 2. This invention further incorporates a core antioxidant system of resveratrol and glutathione encapsulated in a phospholipid-cholesterol structure, enabling the stable coexistence of both water-soluble and fat-soluble components within the same system, while minimizing their activity loss during storage and use. This nano-encapsulation structure exhibits excellent compatibility with the exosome phospholipid membrane, enhancing the overall antioxidant capacity of the system without disrupting the exosome structure, thereby enhancing the maintenance of cellular activity.

[0019] 3. Sodium adenosine triphosphate, as a precursor for cellular metabolism, participates in the energy supply process and synergistically promotes the uptake and action of active exosome components. Ceramide, squalane, and phosphatidylserine create a biomimetic lipid barrier structure, helping to slow water loss and enhance the scalp's surface moisture retention. Plant peptide extracts and plant tannin complexes synergistically regulate inflammatory pathways, while ingredients like niacinamide and sodium polyglutamate support moisturization and stabilize the skin microenvironment.

[0020] 4. The introduction of Polygonum multiflorum extract, rich in bioactive ingredients such as emodin and stilbene glycosides, has been shown to regulate hair follicle cycle, enhance dermal papilla cell activity, and provide antioxidant and anti-aging benefits. It also creates a synergistic effect with exosomes and other conditioning factors. The addition of Polygonum multiflorum extract enhances the potential of the composition for hair loss prevention and conditioning, promoting the maintenance of hair physiological homeostasis, and broadening its applicable population and efficacy areas.

[0021] In summary, the composition of the present invention produces synergistic effects in multiple dimensions such as barrier repair, oxidative stress relief, moisture retention, and scalp microecological stabilization. The technical effect is more systematic and stable, and is suitable for the intervention and long-term conditioning of various types of scalp problems. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a bar chart comparing transepidermal water loss (TEWL) indicators; Figure 2This is a bar chart comparing the water content index of the stratum corneum; Figure 3 This is a bar chart comparing inflammatory factor (IL-1β) indicators; Figure 4 This is a bar chart comparing hair follicle activity scoring indicators. DETAILED DESCRIPTION

[0023] The following examples are provided to further illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise stated, the raw materials used in the examples are all commercially available qualified products.

[0024] Example 1 (T1) This embodiment provides a scalp care composition, which includes the following components by mass percentage: Stem cell-derived exosomes 3%, epithelial cell-derived exosomes 2%, resveratrol 0.1%, glutathione 0.1%, sodium adenosine triphosphate 0.02%, ceramide 0.3%, plant peptide extract 1.2%, plant tannin complex 0.1%, sodium hyaluronate 0.1%, niacinamide 0.3%, sodium polyglutamate 0.1%, phosphatidylserine 0.05%, carnosine 0.02%, hydrolyzed oat β-glucan 0.05%, squalane 0.3%, arginine lactate 0.05%, lipoic acid 0.005%, asiaticoside 0.02%, dipotassium glycyrrhizate 0.05%, bifid yeast fermentation product 0.1%, ferulic acid 0.05%, oat peptide 0.1%, whey protein hydrolyzate 0.1%, Polygonum multiflorum extract 0.1% (obtained by ethanol-water extraction), and the remainder of the carrier matrix is ​​100%.

[0025] The carrier matrix is ​​composed of the following ingredients: 5% glycerol, 4% butylene glycol, 2% propylene glycol, 0.5% Tween 80, 0.1% acetylated sodium hyaluronate, 0.2% lecithin, and the remainder is water.

[0026] The overall pH value of the composition is adjusted to 5.8, the osmotic pressure is controlled at about 285 mOsm / kg, and the shear viscosity is 90 mPa·s.

[0027] The stem cell-derived exosomes are secreted by human adipose stem cells (within three generations, in an animal serum-free environment). The extraction method is ultracentrifugation (100,000×g, 70 minutes) combined with 0.1μm ultrafiltration membrane concentration. The particle size is 85nm and the protein content is 56μg / mL, which is confirmed by positive detection of CD63, CD81, and Tsg101 antibodies.

[0028] The epithelial cell-derived exosomes are derived from human hair follicle epithelial cells (within three generations, in an animal serum-free environment) and are obtained by a combined extraction method of density gradient centrifugation and 0.1 μm hollow fiber ultrafiltration tubes. The particle size is 78 nm, the Zeta potential is −21.3 mV, and the protein content is 41 μg / mL.

[0029] Resveratrol and glutathione were mixed in a 1:1 ratio, dissolved in ethanol containing 0.5% Tween 80, and added to an organic phase prepared with hydrogenated lecithin and cholesterol in a mass ratio of 3:1. The solvent was removed by rotary evaporation to form a lipid film, and then buffer was added for hydration and probe ultrasound was used for 90 seconds to form nanoemulsified inclusions with a particle size of 120 nm.

[0030] The preparation steps of this composition are as follows: S1. Prepare stem cell-derived exosomes and epithelial cell-derived exosomes by ultracentrifugation and membrane separation, respectively. After concentration, store at -80°C for later use. S2. Dissolve the above two exosomes in an aqueous phase containing 5% glycerol and HEPES buffer respectively, and slowly stir and dissolve at 4°C to prepare an exosome aqueous phase system; S3, adding the resveratrol and glutathione complex solution to the phospholipid-cholesterol organic phase to form a lipid film, hydrating and ultrasonically emulsifying the film to form an inclusion emulsion; S4. The exosome system obtained in S2 was slowly added dropwise to the emulsion obtained in S3. After premixing under stirring, the mixture was dispersed by probe-type ultrasonic treatment for 10 minutes (intermittent mode, power 150W). The mixture was then subjected to two high-pressure homogenization treatments (first round at 800 bar and second round at 600 bar) to enhance the encapsulation efficiency and stability, thereby obtaining a uniform exosome-liposome composite dispersion system. S5. Add ceramide, niacinamide, squalane, carnosine, sodium polyglutamate, hydrolyzed oat β-glucan, arginine lactate, sodium adenosine triphosphate, asiaticoside, dipotassium glycyrrhizate, bifid yeast fermentation product, ferulic acid, oat peptides, whey protein hydrolyzate and Polygonum multiflorum extract in sequence, stir evenly and adjust the pH to 5.8. Sterilize and filter using a 0.22μm filter membrane, fill in a quantitative manner and seal.

[0031] Example 2 (T2) This embodiment provides a scalp care composition, which includes the following components by mass percentage: Stem cell-derived exosomes 1%, epithelial cell-derived exosomes 0.5%, resveratrol 0.01%, glutathione 0.01%, sodium adenosine triphosphate 0.005%, ceramide 0.05%, plant peptide extract 0.1%, plant tannin complex 0.01%, sodium hyaluronate 0.01%, niacinamide 0.05%, sodium polyglutamate 0.01%, phosphatidylserine 0.01%, carnosine 0.005%, hydrolyzed oat β-glucan 0.01%, squalane 0.1%, arginine lactate 0.01%, lipoic acid 0.001%, asiaticoside 0.005%, dipotassium glycyrrhizate 0.005%, bifid yeast fermentation product 0.01%, ferulic acid 0.01%, oat peptide 0.01%, whey protein hydrolysate 0.01%, and the remainder of the carrier matrix is ​​100%.

[0032] The carrier matrix is ​​composed of the following ingredients: 1% glycerol, 1% butylene glycol, 0.5% propylene glycol, 0.1% Tween 80, 0.015% acetylated sodium hyaluronate, 0.02% lecithin, and the remainder is water.

[0033] The pH value of the prepared composition was adjusted to 5.2, the osmotic pressure was controlled at 270 mOsm / kg, and the shear viscosity was measured to be 40 mPa·s.

[0034] The stem cell-derived exosomes were extracted from human umbilical cord mesenchymal stem cells, with a particle size of 92 nm and a protein concentration of 21 μg / mL. The CD63, CD81, and Tsg101 antibody test results were positive, indicating that the structure was intact.

[0035] The epithelial cell-derived exosomes are derived from human keratinocytes and are extracted using a 0.25M sucrose density gradient centrifugation combined with a 0.1μm ultrafiltration tube. The particle size is 77nm and the Zeta potential is −18.7mV.

[0036] Resveratrol and glutathione were mixed in a 1:1 ratio, dissolved in ethanol containing 0.2% Tween 80, and then emulsified by adding hydrogenated lecithin and cholesterol in a mass ratio of 2:1 into an organic phase. After rotary evaporation to form a lipid film, buffer solution was added for hydration. The mixture was ultrasonically treated for 60 seconds to form emulsified nanoinclusions with an average particle size of 105 nm.

[0037] The preparation steps are the same as in Example 1.

[0038] Example 3 (T3) This embodiment provides a scalp care composition, which includes the following components by mass percentage: Stem cell-derived exosome 8%, epithelial cell-derived exosome 4%, resveratrol 0.5%, glutathione 0.3%, adenosine triphosphate sodium 0.1%, ceramide 0.5%, plant polypeptide extract 3%, plant tannin compound 0.3%, sodium hyaluronate 0.5%, nicotinamide 1%, polyglutamic acid sodium 0.3%, phosphatidylserine 0.2%, carnosine 0.1%, hydrolyzed oat beta-glucan 0.2%, squalane 1%, arginine lactate 0.3%, lipoic acid 0.05%, asiaticoside 0.05%, dipotassium glycyrrhizinate 0.1%, schizochytrium sp. fermentate 0.2%, ferulic acid 0.1%, oat peptide 0.2%, whey protein hydrolysate 0.2%, radix polygoni multiflori praeparata extract 0.2%, and carrier base up to 100%.

[0039] The carrier base is composed of the following ingredients: glycerin 3%, butylene glycol 2%, propylene glycol 2%, Tween 800.5%, acetylated sodium hyaluronate 0.3%, lecithin 0.3%, and the rest is water.

[0040] The pH of the entire composition is adjusted to 6.5, the osmotic pressure is controlled to about 310 mOsm / kg, and the shear viscosity is measured to be 140 mPa·s.

[0041] The stem cell-derived exosomes are obtained from dental pulp stem cells, cultured without animal-derived components for three generations, pretreated with a 0.22 μm filter, and extracted by ultracentrifugation (120,000 x g, 2 times) combined with polymer precipitation. The average particle size is 145 nm, the protein content is 98 μg / mL, and the exosome marker proteins CD63, CD81, and Tsg101 are all positive.

[0042] The epithelial cell-derived exosomes are derived from hair follicle epithelial cells, and the extraction method is sucrose density gradient centrifugation combined with 0.1 μm hollow fiber ultrafiltration. The particle size distribution is concentrated at about 125 nm, the Zeta potential is −29 mV, and the protein content is 83 μg / mL.

[0043] Resveratrol and glutathione are dissolved in a 1% Tween 80 ethanol solution at a mass ratio of 1:1, an organic phase prepared by mixing hydrogenated lecithin and cholesterol at a mass ratio of 4:1 is added, a lipid film is formed by rotary evaporation, and then phosphate buffer is used for hydration and double-frequency probe ultrasonication (power 150 W, 120 seconds) to prepare an emulsified encapsulation solution with an average particle size of 145 nm.

[0044] The preparation steps are the same as in Example One, except that in the S5 stage, radix polygoni multiflori praeparata extract and other functional extracts are added together, and homogenization and sterilization filtration operations are performed.

[0045] Comparative Example One (C1) This comparative example provides a scalp care composition, which includes the following components by mass percentage: Stem cell-derived exosomes 3%, resveratrol 0.1%, glutathione 0.1%, sodium adenosine triphosphate 0.02%, ceramide 0.3%, plant peptide extract 1.2%, sodium hyaluronate 0.1%, niacinamide 0.3%, squalane 0.3%, and carrier matrix balance to 100%.

[0046] The carrier matrix includes 2% glycerol, 1% butylene glycol, 0.5% propylene glycol, 0.1% Tween 80, 0.1% lecithin, 0.08% acetylated sodium hyaluronate, and the remainder is water.

[0047] The overall pH value of the composition is adjusted to 5.6, the osmotic pressure is about 275 mOsm / kg, and the shear viscosity is about 75 mPa·s. The product is sterilized through a 0.22 μm filter membrane and then filled and stored in a dark environment at 4°C.

[0048] The preparation steps are the same as in Example 1.

[0049] Comparative Example 2 (C2) This comparative example provides a scalp care composition, which comprises the following components by mass percentage: Stem cell-derived exosomes 3%, epithelial cell-derived exosomes 2%, resveratrol 0.1%, glutathione 0.1%, sodium adenosine triphosphate 0.02%, ceramide 0.3%, plant peptide extract 1.2%, plant tannin complex 0.1%, sodium hyaluronate 0.1%, niacinamide 0.3%, sodium polyglutamate 0.1%, phosphatidylserine 0.05%, carnosine 0.02%, hydrolyzed oat β-glucan 0.05%, squalane 0.3%, arginine lactate 0.05%, lipoic acid 0.005%, asiaticoside 0.02%, dipotassium glycyrrhizate 0.05%, bifid yeast fermentation product 0.1%, ferulic acid 0.05%, oat peptide 0.1%, whey protein hydrolysate 0.1%, and the remainder of the carrier matrix is ​​100%.

[0050] The carrier matrix is ​​composed of the following ingredients: 5% glycerol, 4% butylene glycol, 2% propylene glycol, 0.5% Tween 80, 0.1% acetylated sodium hyaluronate, 0.2% lecithin, and the remainder is water.

[0051] The difference from Example 1 is that in this formula, resveratrol and glutathione are directly dissolved in the aqueous phase system and added without phospholipid-cholesterol encapsulation treatment and without forming an emulsified composite structure.

[0052] The pH value of the prepared composition is 5.8, the osmotic pressure is about 285 mOsm / kg, and the shear viscosity is about 90 mPa·s. The finished product is sterilized through a 0.22 μm filter membrane, then filled, sealed, and stored at 4° C. in the dark.

[0053] The preparation steps were basically the same as those in Example 1, except that the emulsification and embedding operation was omitted in the S3 stage.

[0054] Comparative Example 3 (C3) This comparative example provides a scalp care composition, which comprises the following components by mass percentage: Stem cell-derived exosomes 3%, epithelial cell-derived exosomes 2%, resveratrol 0.1%, glutathione 0.1%, hydrolyzed oat β-glucan 0.05%, bifid yeast fermentation product 0.1%, and the balance of carrier matrix to 100%.

[0055] The resveratrol and glutathione are mixed in a ratio of 1:1, and are wrapped with a phospholipid-cholesterol structure to form an emulsified system, with a particle size controlled at about 120 nm.

[0056] This formula does not contain the following ingredients: sodium adenosine triphosphate, ceramide, squalane, phosphatidylserine, plant peptide extract, plant tannin complex, niacinamide, sodium polyglutamate, carnosine, arginine lactate, lipoic acid, asiaticoside, dipotassium glycyrrhizate, ferulic acid, oat peptides, whey protein hydrolysate.

[0057] The carrier matrix includes 2% glycerol, 1% butylene glycol, 0.2% Tween 80, 0.2% lecithin, 0.1% acetylated sodium hyaluronate, 0.3% propylene glycol, and the remainder is water.

[0058] The pH value of the composition is 5.7, the osmotic pressure is about 275 mOsm / kg, and the shear viscosity is about 65 mPa·s. The composition is sterilized through a 0.22 μm filter membrane and then filled and stored at 4° C. in the dark.

[0059] The preparation steps are basically the same as those in Example 1.

[0060] Comparative Example 4 (C4) This comparative example provides a scalp care composition, which comprises the following components by mass percentage: Resveratrol 0.1%, glutathione 0.1%, sodium hyaluronate 0.1%, hydrolyzed oat β-glucan 0.05%, carrier matrix balance to 100%.

[0061] The resveratrol and glutathione are directly added to the aqueous phase system without undergoing phospholipid-cholesterol encapsulation treatment and without forming an emulsified structure.

[0062] This formula does not contain the following ingredients: stem cell-derived exosomes, epithelial cell-derived exosomes, sodium adenosine triphosphate, ceramide, squalane, phosphatidylserine, plant peptide extract, plant tannin complex, niacinamide, sodium polyglutamate, carnosine, arginine lactate, lipoic acid, asiaticoside, dipotassium glycyrrhizate, bifid yeast fermentation product, ferulic acid, oat peptides, and whey protein hydrolysate.

[0063] The carrier matrix includes 0.5% glycerol, 0.5% butylene glycol, 0.1% Tween 80, 0.1% acetylated sodium hyaluronate, 0.1% lecithin, 0.05% propylene glycol, and the remainder is water.

[0064] The pH value of the composition is 5.6, the osmotic pressure is about 270 mOsm / kg, and the shear viscosity is about 45 mPa·s. The composition is sterilized through a 0.22 μm filter membrane and then filled and stored at 4° C. in the dark.

[0065] The preparation steps are basically the same as those in Example 1, with only the purification process in step S1 being changed.

[0066] To validate the comprehensive efficacy of the scalp care composition described herein, an in vivo rat model was used for evaluation. Clean-grade male Sprague-Dawley rats weighing 180-220 g were used. They were acclimated for three days prior to the experiment and maintained at a temperature of 22 ± 2°C, a relative humidity of 55% ± 10%, and a 12-hour / 12-hour light cycle. All animals were numbered according to experimental number and randomly divided into groups.

[0067] The model was established by shaving a 3 x 3 cm area on the rats' backs and applying a 2% sodium dodecyl sulfate (SDS) solution twice daily for three consecutive days to induce skin barrier damage and inflammation. The normal control group (NC) was treated with distilled water alone. After the model was established, interventions were administered to each group starting on day 4.

[0068] Rats were randomly divided into nine groups, with 10 rats in each group: normal control group (NC), Example 1 (T1), Example 2 (T2), Example 3 (T3), Comparative Example 1 (C1), Comparative Example 2 (C2), Comparative Example 3 (C3), Comparative Example 4 (C4) and commercial nursing group (MKT).

[0069] The treatment method is to evenly apply 2mg / cm² of the test composition to the modeling area once a day for 14 consecutive days. After the treatment, the following indicators are tested: (1) Transepidermal water loss (TEWL), which reflects the integrity of the skin barrier; (2) Water content of the stratum corneum, reflecting the hydration status; (3) IL-1β levels in skin tissue, reflecting the degree of inflammation; (4) Hair follicle activity score, a comprehensive assessment based on histological staining and visual observation (0-6 points).

[0070] The data are expressed as mean ± standard deviation, see Table 1. One-way analysis of variance (ANOVA) and Dunnett's post hoc test were used to compare the results with the commercial care group (MKT). P < 0.05 was considered a significant difference, and P < 0.01 was considered an extremely significant difference.

[0071] Table 1 Animal experiment data for efficacy evaluation of the composition The various index data obtained from the animal experiments indicate that the scalp care composition corresponding to Example 1 of the present invention exhibits excellent comprehensive performance.

[0072] In terms of transepidermal water loss (TEWL), the T1 group's value was only 21.4g / m²·h, significantly lower than the 36.1g / m²·h of the commercially available care group (MKT), and also lower than the other examples and all comparative groups. The T1 formula uses a combination of 3% stem cell-derived exosomes and 2% epithelial cell-derived exosomes, combined with a biomimetic lipid structure formed by ceramide and squalane, which can effectively reconstruct the damaged stratum corneum barrier and enhance the stability of intercellular lipids, which is the main reason for reducing water loss.

[0073] In terms of stratum corneum water content, the average value of the T1 group reached 68.2, a significant increase from the 53.8 of the commercially available treatment group, and also superior to the T2 and T3 groups, demonstrating that this formulation not only repairs the barrier but also possesses stronger moisture-locking and moisturizing capabilities. This effect is attributed to the hydrophilic polymer network formed by sodium polyglutamate and sodium hyaluronate within the exosome carrier system, which further maintains moisture balance with the help of ingredients such as phosphatidylserine, effectively combating stratum corneum dryness and structural disorder caused by modeling.

[0074] In terms of inflammation control, the IL-1β content in the skin tissue of the T1 group was 45.6 pg / mg, which was significantly lower than the 70.2 pg / mg of the commercially available care group, indicating that the composition has outstanding performance in anti-inflammatory regulation. It is speculated that this is related to the immune regulatory factors carried in the exosomes, the free radical scavenging system constructed by resveratrol and glutathione, and the auxiliary anti-inflammatory function of the plant tannin complex. The three form a complete inflammation shielding mechanism.

[0075] The T1 group performed best in the hair follicle activity score, with a score of 5.9, close to the full score of 6, much higher than the 4.0 of the commercially available care group, and better than all the examples and comparative groups. Combined with histological observations, this ratio scheme can significantly maintain the morphological structure of hair follicles and promote the continuation of the growth phase, indicating that it has significant advantages in promoting hair growth. In contrast, the comparative examples C1 to C4 performed poorly in all four test indicators due to the lack of exosomes, the absence of biomimetic lipids, or the incomplete moisturizing barrier structure. This was particularly evident in the C3 and C4 groups, verifying that a single functional composition is difficult to achieve a comprehensive repair effect.

[0076] Thus, Example 1 achieves multi-level, multi-component synergy in its formula structure. By precisely designing the exosome concentration and the ratio of the hydrophilic and sealing agents, it exerts a significant combined effect in skin barrier reconstruction, moisture regulation, inflammation relief, and hair follicle activation. Its efficacy is significantly better than existing commercially available products, and the technical effect is significant and creative.

[0077] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and modifications fall within the scope of the invention as claimed.

Claims

1. A scalp care composition, characterized in that Calculated by mass percentage, it includes the following components: 1-8% stem cell-derived exosomes, 0.5-4% epithelial cell-derived exosomes; Resveratrol 0.01-0.5%, glutathione 0.01-0.3%, sodium adenosine triphosphate 0.005-0.1%, ceramide 0.05-0.5%, plant peptide extract 0.1-3%, plant tannin complex 0.01-0.3%, sodium hyaluronate 0.01-0.5%, niacinamide 0.05-1%; Sodium polyglutamate 0.01-0.3%, phosphatidylserine 0.01-0.2%, carnosine 0.005-0.1%, hydrolyzed oat beta-glucan 0.01-0.2%, squalane 0.1-1%, arginine lactate 0.01-0.3%, lipoic acid 0.001-0.05%; The carrier matrix balance is 100%; The carrier matrix includes one or more combinations of water, glycerol, butylene glycol, propylene glycol, Tween 80, acetylated sodium hyaluronate and lecithin, and the pH value of the overall system is controlled at 5.0-6.5, the osmotic pressure is 260-310 mOsm / kg, and the shear viscosity is controlled at 30-150 mPa·s.

2. The scalp care composition according to claim 1, characterized in that The composition is formulated in the following mass percentages: 3% stem cell-derived exosomes, 2% epithelial cell-derived exosomes, 0.1% resveratrol, 0.1% glutathione, 0.02% sodium adenosine triphosphate, 0.3% ceramide, 1.2% plant peptide extract, 0.1% plant tannin complex, 0.1% sodium hyaluronate, 0.3% niacinamide, 0.1% sodium polyglutamate, 0.05% phosphatidylserine, 0.02% carnosine, 0.05% hydrolyzed oat β-glucan, 0.3% squalane, 0.05% arginine lactate, 0.005% lipoic acid, and the remainder of the carrier matrix to 100%.

3. The scalp care composition according to claim 1 or 2, characterized in that The stem cell-derived exosomes are selected from human adipose stem cell exosomes, human umbilical cord mesenchymal stem cell exosomes or dental pulp stem cell exosomes, with a particle size of 30 to 150 nm. The exosome protein content is 20 to 100 μg / mL after detection by CD63, CD81 and Tsg101 antibodies.

4. The scalp care composition according to claim 1 or 2, characterized in that The epithelial cell-derived exosomes are derived from human hair follicle epithelial cells, keratinocytes or their precursor cells, and are extracted by density gradient centrifugation and 0.1 μm ultrafiltration membrane. The Zeta potential is controlled at −15 mV to −30 mV.

5. The scalp care composition according to claim 1, wherein The resveratrol and glutathione are compounded in a ratio of 1:1, dissolved in an ethanol phase containing Tween 80, and then encapsulated by hydrogenated lecithin and cholesterol in a mass ratio of 2:1 to 4:1 to form inclusions with a particle size of 80 to 150 nm, which are then dispersed in an aqueous phase to form a stable emulsion system.

6. The scalp care composition according to claim 1, wherein It also includes one or more of the following optional components: 0.005-0.05% asiaticoside, 0.005-0.1% dipotassium glycyrrhizate, 0.01-0.2% bifida yeast fermentation product, 0.01-0.1% ferulic acid, 0.01-0.2% oat peptide, 0.01-0.2% whey protein hydrolyzate, and 0.01-0.2% Polygonum multiflorum extract.

7. A method for preparing the scalp care composition according to any one of claims 1 to 6, characterized in that: The steps include: S1. Extract stem cell-derived exosomes and epithelial cell-derived exosomes, concentrate them by 0.1 μm ultrafiltration, and freeze them at −80°C until use. S2. Slowly dissolve the exosomes in a system containing glycerol and HEPES buffer and adjust the temperature to 4-8°C; S3, resveratrol and glutathione are mixed in proportion and added to the ethanol phase, dissolved and then slowly emulsified into the phospholipid-cholesterol organic solution, the solvent is removed by rotary evaporation to form a lipid film, and then hydrated to form a composite inclusion emulsion; S4. Add the exosome system obtained in step S2 dropwise to the emulsion obtained in step S3, disperse using ultrasound for 10 minutes, and perform high-pressure homogenization twice; S5. Add ceramide, niacinamide, squalane, plant peptides, sodium polyglutamate, regulators, moisturizing factors and Polygonum multiflorum extract, stir evenly, adjust the pH to 5.5-6.5, sterilize with a 0.22 μm filter membrane, and fill to obtain the finished product.

8. The preparation method according to claim 7, characterized in that Before extracting exosomes in step S1, the cells used were all cultured within three generations in an animal serum-free environment, and the endotoxin detected after exosome extraction was ≤0.5EU / mL.

9. The preparation method according to claim 7, characterized in that The phospholipid emulsion is hydrogenated lecithin and cholesterol in a ratio of 2:1 to 4:1, assisted by Tween 80 and PEG-40 hydrogenated castor oil to form a mixed emulsified phase, and probe ultrasound is used for 60 to 120 seconds to prepare nano-scale emulsified particles.

10. The preparation method according to any one of claims 7 to 9, characterized in that: The finished product is stored at 2-10 DEG C in the dark and is packaged in an aluminum-plastic hose filled with inert gas or a multi-layer emulsion bottle with an air barrier valve.

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