Composition with uric acid reducing effect and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a composition with a uric acid reducing effect and application thereof. The natural adinandra nitida and the natural ampelopsis grossedentata are used as main raw materials, and at least one of the shrimp and crab shell extract, the chitosan, the chitosan oligosaccharide, the celery seed extract and the black cherry extract is used as auxiliary materials, so that the obtained composition is rich in active ingredients such as metoplanin, camelliin, myricetin, dihydromyricetin, apigenin and anthocyanin; the compound has the pharmacological activities of reducing uric acid, resisting inflammation, resisting oxidation, enhancing the vitality of the pineal body and the like. In-vivo and in-vitro experiments show that the composition can effectively reduce the uric acid level in vivo and relieve acute-stage inflammatory response of hyperuricemia, and has the effects of diminishing inflammation, easing pain, dispelling wind, eliminating swelling, clearing heat from throat and the like. In addition, the compound tea does not contain caffeine, does not influence sleep, is free of addiction, is suitable for a wide range of people, and has wide application and development prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a composition with uric acid-lowering effects and applications thereof. Background Art

[0002] Gout is one of the most common autoinflammatory diseases, characterized by elevated serum uric acid levels, which leads to urate deposition in and around joints. It is one of the most common inflammatory arthritis types. According to statistics, hyperuricemia in my country is showing a significant upward trend and is affecting younger people, with the number of patients reaching 177 million and an overall prevalence of 11.3%. Online user surveys show that nearly 60% of hyperuricemia and gout patients are aged 18-35. Gout has become the second most common metabolic disease in my country, second only to diabetes.

[0003] Uric acid, a nitrogen compound synthesized in the liver, is the primary cause of gout and hyperuricemia. With improvements in living standards, changes in dietary habits, and an increased intake of high-purine foods, the incidence of hyperuricemia and gout in my country has been increasing annually, with the prevalence trending towards younger patients. Hyperuricemia is a disease caused by disorders in purine metabolism or uric acid excretion, leading to elevated uric acid levels in the blood. Clinically, hyperuricemia is diagnosed when serum uric acid levels exceed 416 μmol / L in men and 357 μmol / L in women. When serum uric acid concentrations in hyperuricemia are high, it is prone to deposits in joints, forming tophi. Recurrent gout attacks can lead to gouty arthritis, a progressive form of arthritis linked to abnormal activation of the NLRP inflammasome and cellular oxidative stress. Furthermore, it can also contribute to various metabolic syndromes, including hypertension, diabetes, and kidney disease. Therefore, lowering blood uric acid levels, reducing immune inflammatory responses, and minimizing gout complications are key to treating hyperuricemia and gout.

[0004] Currently, the main methods for treating hyperuricemia and gout include reducing uric acid production, increasing uric acid excretion, promoting uric acid decomposition, and alleviating gouty inflammatory reactions. Corresponding drugs have been developed on this basis. For example, allopurinol, a drug that inhibits uric acid production, benzbromarone and probenecid, drugs that promote uricosuric excretion, rasburicase, a drug that promotes uric acid decomposition, and nonsteroidal anti-inflammatory drugs, colchicine, and glucocorticoids, which are anti-inflammatory and analgesic drugs that relieve gouty arthritis. Although these drugs can effectively improve the symptoms of gout patients and lower uric acid levels, their toxic side effects can easily cause adverse reactions in patients. Long-term use can lead to other diseases in patients and affect their quality of life. Therefore, the development of a safe and efficient uric acid-lowering product has important clinical value and social significance. Summary of the Invention

[0005] Therefore, the application provides a composition with a uric acid reducing effect and application thereof.

[0006] In order to achieve the above-mentioned application purposes, the application provides the following technical solutions.

[0007] The application provides a composition, which comprises rock tea, rattan tea and at least one of the following components:

[0008] Shrimp and crab shell extract, chitosan, chitooligosaccharide, celery seed extract, black cherry extract.

[0009] The extraction method of the celery seed extract is as follows: after the celery seeds are dried, the celery seeds are crushed, and then the powder is sieved through a 60-mesh sieve; 1.5 kg of the powder is put into an extraction kettle; the extraction conditions are as follows: CO2 flow rate is 40 L / h, static extraction time is 1.5 h, dynamic extraction time is 2.5 h, extraction kettle temperature is 45 DEG C, extraction pressure is 25 MPa, separation kettle temperature is 35 DEG C, and extraction pressure is 6 MPa; after being freeze-dried and powdered, the celery seed extract is obtained.

[0010] The extraction method of the black cherry extract is as follows: after the black cherries are washed and pitted, the fruit flesh is obtained; 600 g of the fruit flesh is weighed; 3.5 L of anhydrous ethanol is added to the fruit flesh to homogenize the fruit flesh; the homogenized fruit flesh is placed at-20 DEG C for 24 h; the filter residue in the black cherry slurry is filtered through a filter screen; the filter residue is extracted with 80% ethanol for 2 more times; the supernatants are combined; ethanol is removed from the combined supernatants by rotary evaporation at 37 DEG C under reduced pressure; and then the black cherry extract is obtained by freeze-drying and powdering.

[0011] In some embodiments, the composition comprises rock tea, rattan tea, chitosan, chitooligosaccharide, celery seed extract and black cherry extract.

[0012] The application has the following advantages: the high mountain rock tea fresh leaves produced in Xiangqi Mountain in Zhaoping County, Guangxi, are rich in resources of melatonin and apigenin (camellianin, apigenin, etc.) after being fried, have the effects of reducing urea generation, promoting urea decomposition and resisting inflammation, can effectively reduce the uric acid level in serum, and can relieve joint verification reaction; in addition, the wild rattan tea produced in Hunan is rich in myricetin and dihydromyricetin, and the effective components have the pharmacological activities of removing free radicals, resisting inflammation, protecting liver, resisting oxidation and resisting bacteria, can effectively reduce the uric acid level in serum, and can protect liver and kidney functions.

[0013] Based on this, the present invention uses natural shiya tea and natural rattan tea as the main raw materials, supplemented with chitosan, chitosan oligosaccharides, celery seed extract, and black cherry extract. The resulting composition is rich in active ingredients such as metolanin, camellia glycosides, myricetin, dihydromyricetin, apigenin, and anthocyanins, and has pharmacological activities such as lowering uric acid, anti-inflammatory, and antioxidant. In vitro and in vivo experiments have shown that the composition can effectively lower uric acid levels in the body and alleviate the inflammatory response of hyperuricemia in the acute phase, while also having the effects of dispelling wind and swelling, clearing the throat, and relieving sore throat. In addition, this composite tea is caffeine-free, does not affect sleep, is non-addictive, is suitable for a wide range of people, and has broad application and development prospects.

[0014] The present invention has no particular restrictions on the specific types of the shiya tea and the vine tea, and preferably wild shiya tea and wild vine tea. The present invention has no particular restrictions on the origin of the shiya tea and the vine tea. In a specific embodiment of the present invention, the shiya tea comes from the high mountain shiya tea of ​​Xiangqi Mountain in Zhaoping County, Guangxi, and the vine tea is produced in Hengdong, Hunan.

[0015] In some embodiments, the mass ratio of the shiya tea, vine tea, chitosan, chitosan oligosaccharide, celery seed extract and black cherry extract is (5-50): (5-75): (0.5-5): (0-5): (0.5-5): (0-5).

[0016] In some preferred embodiments, the mass ratio of the shiya tea, rattan tea, chitosan, chitosan oligosaccharide, celery seed extract, and black cherry extract is (25-50):(30-65):(3-5):(2-5):(2-5):(2-5). In some specific embodiments, the mass ratio is 25:65:3:2:3:2, 40:40:5:5:2:3, 25:60:5:3:3:4, and 50:30:5:5:5:5.

[0017] In some embodiments, the shrimp and crab shell extract has a molecular weight of 10 kDa to 20 kDa.

[0018] In some embodiments, the degree of deacetylation of the chitosan is greater than or equal to 75%, and the degree of deacetylation of the chitosan oligosaccharide is greater than 90% and the molecular weight is less than 10 kDa.

[0019] In the present invention, the preparation method of the Shiya tea comprises: picking fresh Shiya tea buds and leaves before Grain Rain, frying, and drying. In some specific embodiments, the mass ratio of the buds and leaves in the Shiya tea is 1:1.

[0020] The preparation method of the rattan tea comprises the following steps: picking tender buds and tender leaves of fresh rattan tea before Grain Rain, frying them, and drying them in the sun.

[0021] The present invention also provides the use of the composition in preparing a product having a uric acid-lowering effect.

[0022] The product is tea or a tea beverage.

[0023] The present invention also provides a compound tea made from the composition described in the present invention. The composition can be made into tea or tea beverages according to conventional preparation processes in the art. The dosage form of the tea or tea beverage can be in various forms such as independently packaged tea bags or tea powder, which are convenient to carry and take, safe and environmentally friendly. The packaging specifications of the tea bags of the compound tea are 3 to 5g, and 2 to 3 bags can be consumed daily. The packaging specifications of the tea powder are 2 to 4g, and 2 to 3 bags can be consumed daily. The brewing temperature of the compound tea beverage is 70 to 90 degrees hot water, the amount of water added is 200 to 300 mL, the brewing time of each tea bag is 20 to 30 minutes, and each bag can be brewed 1 to 2 times.

[0024] The composition of the present invention comprises shiya tea, rattan tea, and at least one of the following components: shrimp and crab shell extract, chitosan, chitosan oligosaccharide, celery seed extract, and black cherry extract. Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The composition of the present invention is rich in effective active ingredients such as metolanin, camelliaside, myricetin, dihydromyricetin, apigenin, anthocyanins, etc., and has the effects of lowering uric acid, anti-inflammatory and swelling, and protecting liver and kidney functions;

[0026] 2. The tea or tea beverage prepared from the composition of the present invention does not contain caffeine, does not affect sleep, is non-addictive, and has a wider audience;

[0027] 3. The tea or tea beverage prepared from the composition of the present invention can be in various forms such as individually packaged tea bags or tea powder, which are convenient to carry and take, safe and environmentally friendly. DETAILED DESCRIPTION

[0028] The present invention provides a composition having a uric acid-lowering effect and its application. Those skilled in the art can refer to the contents of this article and appropriately improve the process parameters to achieve the same. It should be noted in particular that all similar replacements and modifications are obvious to those skilled in the art and are considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0029] The test materials used in the present invention are all common commercial products and can be purchased in the market.

[0030] The present invention will be further described below in conjunction with the embodiments:

[0031] Example 1

[0032] Prepare the tea according to the following recipe:

[0033]

[0034]

[0035] Extraction method of celery seed extract: After the celery seeds are dried, crushed, and passed through a 60-mesh sieve, 1.5 kg of powder is weighed and put into an extraction kettle. The extraction conditions are: CO2 flow rate is 40 L / h, static extraction time is 1.5 h, dynamic extraction time is 2.5 h, extraction kettle temperature is 45 ° C, extraction pressure is 25 MPa, separation kettle temperature is 35 ° C, extraction pressure is 6 MPa, and celery seed extract is obtained after freeze drying and pulverization.

[0036] Extraction method of black cherry extract: wash the black cherries, remove the pits and obtain the pulp, weigh 600g of fresh pulp sample, add 3.5L of anhydrous ethanol to homogenize, and let it stand at -20℃ for 24 hours. Filter the residue in the black cherry pulp through a filter, extract the filtered residue twice with 80% ethanol, combine the supernatants, remove the ethanol by reduced pressure rotary evaporation at 37℃, and then freeze-dry and pulverize to obtain the black cherry extract.

[0037] Weigh all the above ingredients by weight, crush, and mix thoroughly. Aseptically dispense into 5g packets. Brew with 300mL of 90°C boiling water for 30 minutes. Consume one packet each morning and evening.

[0038] Example 2

[0039] Prepare the tea according to the following recipe:

[0040]

[0041] Extraction method of celery seed extract: same as Example 1

[0042] Extraction method of black cherry extract: same as Example 1

[0043] Weigh all the above ingredients by weight, crush, and mix thoroughly. Aseptically dispense 4g / packet. Brew with 200mL of boiling water at approximately 85°C for 20 minutes. Each pack should be infused twice daily, consuming one pack each morning and evening.

[0044] Example 3

[0045] Prepare the tea according to the following recipe:

[0046]

[0047]

[0048] Extraction method of celery seed extract: same as Example 1

[0049] Extraction method of black cherry extract: same as Example 1

[0050] Weigh each component according to weight ratio, crush under liquid nitrogen, and then filter through a 30-mesh filter and sun-dry. Aseptically package the mixture at 3g per packet. Infuse the mixture with 300mL of boiling water at 90°C for 30 minutes. Consume one packet each morning and evening.

[0051] Example 4

[0052] Prepare the tea according to the following recipe:

[0053]

[0054] Extraction method of celery seed extract: same as Example 1

[0055] Extraction method of black cherry extract: same as Example 1

[0056] Weigh all the above ingredients by weight, crush, and mix thoroughly. Aseptically dispense into 5g packets. Brew with 200mL of boiling water at approximately 80°C for 20 minutes. Each packet should be infused twice daily, consuming one packet each morning and evening.

[0057] Comparative Example 1

[0058] Prepare the tea according to the following recipe:

[0059] 45 servings of wild cliff tea

[0060] 55 servings of wild vine tea

[0061] Weigh all the above ingredients by weight, crush, and mix thoroughly. Aseptically dispense into 5g packets. Brew with 200mL of boiling water at approximately 80°C for 20 minutes. Each packet should be infused twice daily, consuming one packet each morning and evening.

[0062] Comparative Example 2

[0063] Prepare the tea according to the following recipe:

[0064]

[0065] Extraction method of celery seed extract: same as Example 1

[0066] Extraction method of black cherry extract: same as Example 1

[0067] Weigh all the above ingredients by weight, crush, and mix thoroughly. Aseptically dispense into 5g packets. Brew with 200mL of boiling water at approximately 80°C for 20 minutes. Each packet should be infused twice daily, consuming one packet each morning and evening.

[0068] Comparative Example 3

[0069] Prepare the tea according to the following recipe:

[0070]

[0071] Extraction method of celery seed extract: same as Example 1

[0072] Extraction method of black cherry extract: same as Example 1

[0073] Weigh all the above ingredients by weight, crush, and mix thoroughly. Aseptically dispense into 5g packets. Brew with 200mL of boiling water at approximately 80°C for 20 minutes. Each packet should be infused twice daily, consuming one packet each morning and evening.

[0074] Comparative Example 4

[0075] The composition was prepared according to the following formula:

[0076] 40 parts of chitosan oligosaccharide

[0077] 35 servings of Celery Seed Extract

[0078] Black cherry extract 25 parts

[0079] Extraction method of celery seed extract: same as Example 1

[0080] Extraction method of black cherry extract: same as Example 1

[0081] Weigh all the above ingredients by weight and mix thoroughly. Aseptically dispense 5g / packet. Infuse with 200mL of boiling water at approximately 80°C for 20 minutes. Each pack should be infused twice daily, consuming one pack each morning and evening.

[0082] Efficacy trials

[0083] The composite teas prepared in Examples 1 to 4 and Comparative Examples 1 to 4 were extracted by soaking in boiling water and then distilled under reduced pressure to 5 g / bag. The teas were numbered as samples 1 to 8 in sequence.

[0084] Experimental animals: 120 Kunming mice aged 5 to 6 weeks, weighing 22 to 26 g, half male and half female, were randomly divided into 12 groups, with 10 mice in each group.

[0085] Experimental groups: Each group of experiments was divided into a blank control group, a model group, an experimental group (Example 1 to 4 groups, Comparative Example 1 to 4 groups), and an experimental control group (allopurinol, benzbromarone).

[0086] Dosage regimen: Oral gavage was used at a dose of 1 mL / 10 g body weight once daily for 3 consecutive weeks. All groups except the blank control group, which was given normal saline, were modeled. The model group (200 mg / kg adenine and 250 mg / kg ethambutol were administered in the morning, followed by an equal volume of normal saline in the afternoon) and the experimental and control groups were all modeled in the morning and administered in the afternoon (Li Guangzhi, Lu Zhongying, et al., Uric acid-lowering effect of dihydromyricetin from vine tea on a mouse model of hyperuricemia, Journal of Mountain Agriculture and Biology, 33(4): 040-042, 2014).

[0087] Measurement Indicators: After three weeks of continuous administration, blood was collected by enucleation and centrifuged to obtain serum for determination of uric acid (UA), uric acid nitrogen (BUN), and creatinine (Cr). Data were analyzed using Origin 8.0 software with t-tests between two groups and one-way analysis of variance for multiple groups. P < 0.05 was considered significant.

[0088] Test results:

[0089] Table 1. Changes in serum uric acid (UA) levels in mice in each group after 3 weeks of administration

[0090]

[0091] Note: *p<0.05, indicating that there are significant differences between the experimental group, the control group and the model group.

[0092] The above results show that compared with the hyperuricemia model group, each drug-administered group can significantly reduce the uric acid level in mice. At the same time, compared with the experimental control group, the composite tea of ​​Examples 1 to 4 has a significant uric acid-lowering effect, and the effect is significant due to the lack of comparative examples 1 to 4. It can reduce kidney damage caused by hyperuricemia, has good kidney protection function, reduces the adverse reactions of drugs, is highly safe, and is suitable for long-term use. It shows that the components of the composition of the present invention have a synergistic effect and are indispensable.

[0093] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition, characterized in that The invention comprises at least one of rock cliff tea, rattan tea and the following components: Shrimp and crab shell extract, chitosan, chitosan oligosaccharide, celery seed extract, black cherry extract; The celery seed extract is extracted by the following method: drying the celery seeds, crushing them, and passing them through a 60-mesh sieve; weighing 1.5 kg of powder and putting it into an extraction kettle; and extracting under the following conditions: a CO2 flow rate of 40 L / h, a static extraction time of 1.5 hours, a dynamic extraction time of 2.5 hours, an extraction kettle temperature of 45° C., an extraction pressure of 25 MPa, a separation kettle temperature of 35° C., an extraction pressure of 6 MPa; and freeze-drying and pulverizing the celery seed extract. The black cherry extract is extracted by washing black cherries, removing pits, and obtaining pulp; weighing 600 g of a fresh pulp sample; adding 3.5 L of anhydrous ethanol to homogenize the pulp; and standing the pulp at -20°C for 24 hours; filtering the residue in the black cherry pulp through a filter; extracting the filtered residue twice with 80% ethanol; combining the supernatants; and removing the ethanol by rotary evaporation under reduced pressure at 37°C; followed by freeze drying and pulverization to obtain the black cherry extract.

2. The composition according to claim 1, characterized in that The composition comprises shiya tea, vine tea, chitosan, chitosan oligosaccharide, celery seed extract and black cherry extract.

3. The composition according to claim 2, characterized in that The mass ratio of the shiya tea, vine tea, chitosan, chitosan oligosaccharide, celery seed extract and black cherry extract is (5-50): (5-75): (0.5-5): (0-5): (0.5-5): (0-5).

4. The composition according to any one of claims 1 to 3, characterized in that The deacetylation degree of the chitosan is greater than or equal to 75%, and the deacetylation degree of the chitosan oligosaccharide is greater than 90% and the molecular weight is less than 10kDa.

5. The composition according to any one of claims 1 to 3, characterized in that The preparation method of the cliff tea comprises the following steps: picking tender buds and tender leaves of fresh cliff tea before Grain Rain, frying them, and drying them in the sun.

6. The composition according to claim 5, characterized in that The mass ratio of tender branches to tender leaves in the Shiya tea is 1:

1.

7. The composition according to any one of claims 1 to 3, characterized in that The preparation method of the rattan tea comprises the following steps: picking tender buds and tender leaves of fresh rattan tea before Grain Rain, frying them, and drying them in the sun.

8. Use of the composition according to any one of claims 1 to 7 in the preparation of a product having a uric acid-lowering effect.

9. The use according to claim 8, characterized in that The product is tea or a tea beverage.

10. A compound tea, characterized in that: Made from the composition according to any one of claims 1 to 7.

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