Feeding training method for wild euchiloglanis kishinouyei

Abstract

The invention relates to the technical field of wild euchiloglanis kishinouyei domestication methods, in particular to a wild euchiloglanis kishinouyei domestication method. According to the feeding training method for the wild euchiloglanis kishinouyei, cobblestones attached with microalgae are adopted to simulate the feeding environment of the wild euchiloglanis kishinouyei. According to the characteristic that wild euchiloglanis kishinouyei has a scraper type ingestion mode, microalgae are cultivated and attached to cobblestones, the cobblestones attached with the microalgae are utilized, the natural ingestion environment of euchiloglanis kishinouyei at the bottom of a river is simulated, the wild euchiloglanis kishinouyei can open firstly for ingestion in an artificial breeding environment, the breeding environment can be adapted as soon as possible, the stress reaction is reduced, and the breeding efficiency is improved. The feeding training success rate of the wild euchiloglanis kishinouyei is greatly increased, and the survival rate of the wild euchiloglanis kishinouyei

Description

Technical Field

[0001] The invention relates to the technical field of wild catfish taming and feeding methods, and in particular to a wild catfish taming and feeding method. Background Art

[0002] Pareuchiloglanis anteanalis (Fang, Xu et Cui, 1984) is an endemic and commercially important fish species found primarily in the Jinsha, Dadu, and Bailong rivers. However, in recent years, human activities have impacted its habitat, leading to a gradual decline in its range and a sharp decrease in its natural reproduction, resulting in a drastic decline in its wild population. Wild resources of Pareuchiloglanis anteanalis are now extremely difficult to obtain, becoming an occasional species. Fish stocks are extremely scarce, nearly endangered, and this has hindered ex situ conservation efforts, including artificial propagation and the establishment of artificial populations. While this situation has been somewhat alleviated by measures such as fishing bans, research on the species remains largely unresolved, with only reports examining its morphological characteristics. The technology of artificial domestication of wild catfish is still in the research stage. Feeding is one of the difficulties of artificial domestication. In the early stage of artificial domestication, it was found that wild catfish have strong stress response, refuse to eat in the artificial breeding environment, become emaciated or even die, resulting in a low survival rate of domestication. Summary of the Invention

[0003] Therefore, the technical problem to be solved by the present invention is to overcome the defect of the survival rate of wild catfish artificially cultivated in the prior art, thereby providing a method for taming wild catfish.

[0004] To this end, the present invention provides a feeding method for wild catfish, which uses pebbles with microalgae attached to simulate the feeding environment of the wild catfish.

[0005] In some embodiments, a method for preparing pebbles with attached microalgae includes the following steps: multiplying microalgae to form a microalgae liquid, and soaking the pebbles in the microalgae liquid, wherein the microalgae include at least one of Chlorella, diatoms, and cyanobacteria.

[0006] It should be noted that the present invention does not limit the source of the microalgae, as long as it can be a food source for wild catfish.

[0007] In some embodiments, the microalgae liquid further includes feed, microbial agents and multivitamin preparations.

[0008] In some embodiments, the weight of the pebbles is 4-5 kg / piece, and bait nests with a diameter of 5-8 cm are provided on the surface of the pebbles, and the number of the bait nests is 1-3.

[0009] In some embodiments, the specific steps of propagating microalgae to form a microalgae liquid include: in the presence of feed, mixing microalgae, microbial agents and a multivitamin preparation to form a microalgae liquid, the cultivation time is 3-5 days, and the amount of microalgae added is 1000-1500 mL / m 3 The amount of microbial agent added is 2000-3000g / m 3 The addition amount of multivitamin preparation is 600-800g / m 3 .

[0010] In some embodiments, the microbial agent includes at least one of Bacillus subtilis or Bacillus licheniformis.

[0011] In some embodiments, during the process of microalgae reproduction to form microalgae liquid, the feed is supplemented once every 10-15 days.

[0012] In some embodiments, the pebbles are immersed in the microalgae liquid while the microalgae liquid is circulating, the circulation flow rate is 0.05-0.1 m / s, and the immersion time is 10-15 days.

[0013] In some embodiments, the method for taming wild catfish includes the following steps: S1, using pebbles with microalgae attached to lure the wild catfish to feed; S2, feeding the wild catfish with an initial feed, which includes 10-15wt% diatom liquid, 40-45wt% fresh shrimp paste, 15-20wt% Chlorella and 5-10wt% Schizochytrium; S3, feeding the wild catfish with a mid-term feed, which includes 5-10wt% diatom liquid, 20-25wt% fresh shrimp paste, 10-15wt% Chlorella and 3-5wt% Schizochytrium; S4, taming the wild catfish with the feed.

[0014] In some embodiments, the feeding period in step S1 is 15-20 days.

[0015] In some embodiments, the feeding time of the initial feed in step S2 is 15-20 days.

[0016] In some embodiments, the feeding time of the mid-term feed in step S3 is 5-10 days.

[0017] In some embodiments, the frequency of feeding the feed in step S2 and / or step S3 is 3-4 times / day, and the amount of the feed is 2-3 wt% based on the total weight of the trained catfish.

[0018] In some embodiments, the concentration of the diatom solution in step S2 and / or step S3 is 100,000-150,000 cells / mL, and the concentration of Chlorella is 1×10 8-1×10 10 / g, the concentration of Schizochytrium was 1×10 8 -1×10 10 pcs / g.

[0019] In some embodiments, the step S4 further includes knocking the outer wall of the culture pond for at least 1 minute before feeding the feed.

[0020] In some embodiments, the amount of pebbles with microalgae attached added in step S1 is 3-5 pebbles / m 2 .

[0021] In some embodiments, in step S4, the wild catfish is tamed by feeding at fixed points.

[0022] The technical solution of the present invention has the following advantages:

[0023] 1. The present invention provides a method for taming wild catfish, using pebbles with attached microalgae to simulate their feeding environment. Based on the fact that wild catfish have a scraping-like feeding habit, the method cultivates attached microalgae on pebbles and uses the pebbles with attached microalgae to simulate the natural feeding environment of catfish on the riverbed. This method enables wild catfish to start feeding early in an artificial breeding environment, quickly adapting to the breeding environment and reducing stress responses. This method significantly improves the success rate of feeding taming and the survival rate of wild catfish.

[0024] 2. The present invention provides a method for taming wild catfish, which comprises the following steps: S1, using pebbles with microalgae attached to lure the wild catfish to feed; S2, feeding the wild catfish with an initial feed, wherein the initial feed comprises 10-15wt% diatom liquid, 40-45wt% fresh shrimp paste, 15-20wt% Chlorella and 5-10wt% Schizochytrium; S3, feeding the wild catfish with a mid-term feed, wherein the mid-term feed comprises 5-10wt% diatom liquid, 20-25wt% fresh shrimp paste, 10-15wt% Chlorella and 3-5wt% Schizochytrium; S4, taming the wild catfish with the feed. The present invention first utilizes pebbles with attached microalgae to lure wild catfish to feed, and then utilizes the omnivorous nature of the catfish to mix diatom liquid, fresh shrimp paste, Chlorella powder, and Schizochytrium powder into the feed, which not only greatly improves the feeding appetite of the catfish, but also improves the nutritional content of the feed, and improves the survival rate and growth rate of the catfish.

[0025] 3. The method for domesticating wild catfish provided by the present invention provides a technical basis for the large-scale domestication of catfish, and also provides a technical reference for the domestication of small benthic fish in the Yangtze River, with significant ecological benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0027] Figure 1 This is a schematic diagram of the structure of the cultivation system in Preparation Example 1 of the present invention;

[0028] Reference numerals:

[0029] 1-water inlet pipe; 2-fertilizer water fermentation tank; 3-strip cultivation tank; 4-water collection tank; 5-water pump. DETAILED DESCRIPTION

[0030] The following examples are provided for a better understanding of the present invention and are not intended to limit the best mode of implementation. They do not limit the content and scope of protection of the present invention. Any product identical or similar to the present invention obtained by anyone under the guidance of the present invention or by combining the features of the present invention with other prior arts shall fall within the scope of protection of the present invention.

[0031] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in this field can be used. If the manufacturer of the reagents or instruments is not specified, they are all commercially available conventional reagents.

[0032] The main components of the feed and compound vitamin preparations in the preparation examples and embodiments of the present invention are as follows:

[0033] Multivitamin preparations include 10,000-20,000 IU / g of vitamin A, 3,000-5,000 IU / g of vitamin D, 100-200 mg / g of vitamin E, 5-20 mg / g of vitamin B1, 5-20 mg / g of vitamin B2, 5-10 mg / g of vitamin B6, 0.01-0.1 mg / g of vitamin B12, 300-500 mg / g of vitamin C, 5-10 mg / g of vitamin K, 5-10 mg / g of folic acid, 30-50 mg / g of pantothenic acid, 50-100 mg / g of niacinamide, and 0.5-1 mg / g of biotin;

[0034] The feed meets the following conditions: the mass content of crude protein is ≥42%, the mass content of crude fat is ≥10%, the mass content of crude fiber is ≤8%, the mass content of crude ash is ≤20%, the mass content of total phosphorus is ≥2%, the mass content of lysine is ≥3%, and the mass content of water is ≤12%.

[0035] Preparation Example 1

[0036] This preparation example provides a method for preparing pebbles with attached microalgae. The specific steps and methods are as follows:

[0037] (1) Select 4kg / piece of smooth pebbles, clean them and soak them in 5ppm potassium permanganate for 2h, clean them and dry them in the sun, and then use a cutting machine to cut 3 circular bait nests with a diameter of 6cm on the surface of the pebbles. The bait nests are in the shape of pot bottoms, and then cut them into 4 pieces / m 2 The amount of pebbles added is to place the pebbles into the strip cultivation pool in the cultivation system for cultivation.

[0038] See also Figure 1 As shown, the cultivation system includes a fertilizer water fermentation tank 2, a strip cultivation tank 3, an oxygenation system, a water collection tank 4, and a water pump 5, wherein the fertilizer water fermentation tank is 1m long, 1m wide, and 0.5m deep, and an oxygenation device is provided at the bottom of the fertilizer water fermentation tank. The fertilizer water fermentation tank is connected to the strip cultivation tank, and an overflow weir is provided on the connecting wall; the overall length and width of the strip cultivation tank are 8m and the depth is 0.4m. The interior of the strip cultivation tank is composed of multiple U-shaped tanks, each U-shaped tank is 8m long, 0.8m wide, and 0.4m deep; the cultivation end of the strip tank is connected to the water collection tank, which is 1m long, 1m wide, and 0.5m deep. A water pump is set up in the tank to pump water to the starting end of the strip cultivation tank with a flow rate of 0.2L / s.

[0039] (2) Continue reading Figure 1 When in use, open the water inlet pipe 1 of the fertilizer water fermentation tank, fill the cultivation system with water, then pour 15kg of feed into the fertilizer water fermentation tank, turn on the aeration and oxygenation device, stir the fertilizer water fermentation tank 3 times a day to make the feed fully dissolved in the water; after 3 days, when the feed is completely dissolved, the water body is earthy yellow. According to the concentration of Chlorella (1×10 6 / mL), diatoms (concentration 1×10 6 / mL) and cyanobacteria (concentration 1×10 6 Chlorella, diatoms and cyanobacteria were mixed as microalgae sources at a mass ratio of 1:1:1, and the microalgae were collected at a rate of 1500 mL / m 3 The microalgae source is connected to the fertilizer water fermentation tank at an addition rate of 1000g / m 3 Bacillus subtilis (the concentration of bacterial powder is 1×1012 CFU / g) and Bacillus licheniformis (the concentration of bacterial powder is 1×10 12 CFU / g), that is, the amount of microbial agent added is 2000g / m 3 , according to 600g / m 3Add a multivitamin preparation to the fertilizer water fermentation tank. After 5 days of cultivation, when the fertilizer water in the fertilizer water fermentation tank turns green, use a water pump to pump the fertilizer water in the fertilizer water fermentation tank twice to the strip cultivation tank. At the same time, turn on the water pump of the water collection tank to circulate the water in the cultivation system at a flow rate of 0.1m / s.

[0040] Feed should be added to the fertilizer and water fermentation tank once every 10 days, based on the last time feed was added. When the feed is first added, the water inlet to the fertilizer and water fermentation tank should be closed. After the feed is completely dissolved and the water turns khaki, the fertilizer and water should be pumped into the strip culture tank twice. At the same time, water should be replenished regularly based on the evaporation rate of the culture system.

[0041] After the pebbles were cultivated in the cultivation system for 10 days, a layer of microalgae attached to the surface of the pebbles was formed.

[0042] Preparation Example 2

[0043] This preparation example provides a method for preparing pebbles with attached microalgae. The specific steps and methods are as follows:

[0044] (1) Select 5kg / piece of smooth pebbles, clean them and soak them in 5ppm potassium permanganate for 2h, clean them and dry them in the sun, and then use a cutting machine to cut two circular bait nests with a diameter of 5cm on the surface of the pebbles. The bait nests are in the shape of pot bottoms, and then cut them into 5 pieces / m 2 The amount of pebbles added is to place the pebbles into the strip cultivation pool in the cultivation system for cultivation.

[0045] The cultivation system includes a fertilizer water fermentation tank, a strip cultivation tank, an oxygenation system, a water collection tank, and a water pump. The fertilizer water fermentation tank is 1m long, 1m wide, and 0.5m deep. An oxygenation device is provided at the bottom of the fertilizer water fermentation tank. The fertilizer water fermentation tank is connected to the strip cultivation tank, and an overflow weir is provided on the connecting wall. The overall length and width of the strip cultivation tank are 8m and the depth is 0.4m. The interior of the strip cultivation tank is composed of multiple U-shaped tanks, each of which is 8m long, 0.8m wide, and 0.4m deep. The cultivation end of the strip tank is connected to the water collection tank, which is 1m long, 1m wide, and 0.5m deep. A water pump is set up in the tank to pump water to the starting end of the strip cultivation tank, and the flow rate is 0.2L / s.

[0046] (2) When in use, open the water inlet pipe of the fertilizer water fermentation tank, fill the cultivation system with water, then pour 20kg of feed into the fertilizer water fermentation tank, turn on the aeration and oxygenation device, stir the fertilizer water fermentation tank 4 times a day to make the feed fully dissolved in the water body; after 3 days, when the feed is completely dissolved, the water body is khaki, and the feed is saturated at 1500mL / m 3 The amount of Chlorella added (concentration 1×10 6 / mL) into the fertilizer water fermentation tank, and then at 1000g / m 3Bacillus subtilis (the concentration of bacterial powder is 1×1012 CFU / g) and Bacillus licheniformis (the concentration of bacterial powder is 1×10 12 CFU / g), that is, the amount of microbial agent added is 2000g / m 3 , according to 600g / m 3 Add a multivitamin preparation to the fertilizer water fermentation tank. After 4 days of cultivation, when the fertilizer water in the fertilizer water fermentation tank turns green, use a water pump to pump the fertilizer water in the fertilizer water fermentation tank twice to the strip cultivation tank. At the same time, turn on the water pump of the water collection tank to circulate the water in the cultivation system with a flow rate of 0.1m / s.

[0047] Feed should be added to the fertilizer and water fermentation tank once every 10 days, based on the last time feed was added. When the feed is first added, the water inlet to the fertilizer and water fermentation tank should be closed. After the feed is completely dissolved and the water turns khaki, the fertilizer and water should be pumped into the strip culture tank twice. At the same time, water should be replenished regularly based on the evaporation rate of the culture system.

[0048] After the pebbles were cultivated in the cultivation system for 10 days, a layer of microalgae attached to the surface of the pebbles was formed.

[0049] Preparation Example 3

[0050] This preparation example provides a method for preparing pebbles with attached microalgae. The specific steps and methods are as follows:

[0051] (1) Select 4kg / piece of smooth pebbles, clean them and soak them in 5ppm potassium permanganate for 2h, clean them and dry them in the sun, and then use a cutting machine to cut a circular bait nest with a diameter of 8cm on the surface of the pebbles. The bait nest is in the shape of a pot bottom, and then place 4 pieces / m 2 The amount of pebbles added is to place the pebbles into the strip cultivation pool in the cultivation system for cultivation.

[0052] The cultivation system includes a fertilizer water fermentation tank, a strip cultivation tank, an oxygenation system, a water collection tank, and a water pump. The fertilizer water fermentation tank is 1m long, 1m wide, and 0.5m deep. An oxygenation device is provided at the bottom of the fertilizer water fermentation tank. The fertilizer water fermentation tank is connected to the strip cultivation tank, and an overflow weir is provided on the connecting wall. The overall length and width of the strip cultivation tank are 8m and the depth is 0.4m. The interior of the strip cultivation tank is composed of multiple U-shaped tanks, each of which is 8m long, 0.8m wide, and 0.4m deep. The cultivation end of the strip tank is connected to the water collection tank, which is 1m long, 1m wide, and 0.5m deep. A water pump is set up in the tank to pump water to the starting end of the strip cultivation tank, and the flow rate is 0.2L / s.

[0053] (2) When in use, open the water inlet pipe of the fertilizer and water fermentation tank, fill the cultivation system with water, then pour 15kg of feed into the fertilizer and water fermentation tank, turn on the aeration and oxygenation device, and stir the fertilizer and water fermentation tank 3 times a day to make the feed fully dissolved in the water body; after 3 days, when the feed is completely dissolved, the water body is earthy yellow. 7 / mL), diatoms (concentration 1×10 7 / mL) and cyanobacteria (concentration 1×10 7 Chlorella, diatoms and cyanobacteria were mixed as microalgae sources at a mass ratio of 2:1:1, and the microalgae were collected at a rate of 1000 mL / m 3 The microalgae source is connected to the fertilizer water fermentation tank at an addition rate of 1500g / m 3 Bacillus subtilis (concentration of bacterial powder was 1×10 8 CFU / g) and Bacillus licheniformis (the concentration of bacterial powder was 1×10 8 CFU / g), that is, the amount of microbial agent added is 3000g / m 3 , according to 800g / m 3 Add a multivitamin preparation to the fertilizer water fermentation tank. After 3 days of cultivation, when the fertilizer water in the fertilizer water fermentation tank turns green, use a water pump to pump the fertilizer water in the fertilizer water fermentation tank twice to the strip cultivation tank. At the same time, turn on the water pump of the water collection tank to circulate the water in the cultivation system with a flow rate of 0.05m / s.

[0054] Feed should be added to the fertilizer and water fermentation tank once every 10 days, based on the last time feed was added. When the feed is first added, the water inlet to the fertilizer and water fermentation tank should be closed. After the feed is completely dissolved and the water turns khaki, the fertilizer and water should be pumped into the strip culture tank twice. At the same time, water should be replenished regularly based on the evaporation rate of the culture system.

[0055] After the pebbles were cultivated in the cultivation system for 15 days, a layer of microalgae attached to the surface of the pebbles was formed.

[0056] Example 1

[0057] This embodiment provides a method for taming wild catfish, and the specific steps and methods are as follows:

[0058] (1) Food induction:

[0059] The pebbles with microalgae attached prepared in Preparation Example 1 were transferred into a 2m diameter culturing pond for the anterior rump catfish. The amount of pebbles with microalgae attached added was 4 pebbles / m 2 , placed side by side, and after the attached microalgae were scraped off by the wild catfish, pebbles with attached microalgae were added, and this process lasted for 20 days.

[0060] (2) Preparation of feed:

[0061] A feed with a particle size of 0.6-1.0 mm was selected, and diatom liquid (concentration of 100,000 particles / mL) was added at an amount of 10 wt% based on the total weight of the feed. After thorough stirring, the mixture was air-dried in a cool place for 1-2 hours. Then, the live freshwater shrimp were headed and minced into fresh shrimp paste using a meat grinder. The fresh shrimp paste was mixed with the air-dried feed, and Chlorella powder and Schizochytrium powder were added while stirring. The Chlorella powder (concentration of 1×10 9 / g) was added in an amount of 20 wt%, and Schizochytrium powder (concentration 1×10 9 The amount of the raw material of the raw material is 10 wt % and the amount of the fresh shrimp paste is 40 wt %. After stirring evenly, the raw material is dried in a cool place for 30 minutes to form the initial feed.

[0062] A feed with a particle size of 0.6-1.0 mm was selected, and a diatom liquid (concentration of 100,000 diatoms / mL) was added at a rate of 5 wt% based on the total weight of the feed. After thorough stirring, the feed was aired in a cool place for 1-2 hours. Then, the live freshwater shrimp were headed and minced into fresh shrimp paste using a meat grinder. The fresh shrimp paste was mixed with the air-dried feed, and Chlorella powder and Schizochytrium powder were mixed while stirring. The fresh shrimp paste, Chlorella powder, and Schizochytrium powder were mixed, wherein the Chlorella powder (concentration of 1×10 9 / g) was added in an amount of 10 wt%, and Schizochytrium powder (concentration 1×10 9 The addition amount of fresh shrimp paste is 20wt%, and the mixture is evenly mixed and aired in a cool place for 30 minutes to form a medium-term feed.

[0063] (3) Mixed feeding:

[0064] A PVC tube with a diameter of 50 mm is placed on the bait nest of pebbles, and the initial feed after airing in step (2) is added from the other end of the PVC tube. After the feed is transported, the PVC tube is removed. The amount of the initial feed after airing is 2 wt% of the total weight of the front rump catfish in the pond. The feed is fed three times a day, and the remaining feed is discharged from the breeding pond after feeding for 40 minutes;

[0065] After feeding the above feed for 15 days, the feed was changed to the mid-term feed and continued to be fed for 5 days. The feeding method, feeding frequency and feeding amount remained unchanged.

[0066] (4) Full feeding:

[0067] After 40 days of acclimation, diatom liquid, fresh shrimp paste, Chlorella powder and Schizochytrium powder are no longer added. At this time, wild catfish can fully consume compound feed. Before feeding, gently tap the outer wall of the breeding pond to form a feeding signal. Keep sending the signal for 1 minute before each feeding, and then start feeding. After 4-5 weeks of training, the fish will form a conditioned reflex to swim to the feeding point upon hearing the signal, thereby improving feeding efficiency and feed utilization. The specific steps of complete feeding include using a fixed-point feeding method, feeding in multiple bait nests on pebbles to ensure that the fish feed evenly, and following the "slow-fast-slow" rhythm when feeding. Feed a small amount first to attract the fish to gather, and then speed up the feeding. When the fish feeding becomes slow, slowly reduce the feeding amount to avoid water pollution caused by the feed being soaked in water for a long time.

[0068] After 100 days of complete feeding, the number of surviving wild catfish was recorded. According to the survival rate = the number of surviving wild catfish after domestication / the total number of wild catfish participating in the domestication × 100%, it was calculated that the survival rate of wild catfish using the domestication method provided in this embodiment was 85%.

[0069] Example 2

[0070] This embodiment provides a method for taming wild catfish, and the specific steps and methods are as follows:

[0071] (1) Food induction:

[0072] The pebbles with microalgae attached prepared in Preparation Example 1 were transferred into the culturing pond of the anterior rump catfish (2 m in diameter) and the amount of pebbles with microalgae attached added was 5 pebbles / m 2 , placed side by side, and after the attached microalgae were scraped off by the wild catfish, pebbles with attached microalgae were added, and this process lasted for 15 days.

[0073] (2) Preparation of feed:

[0074] A feed with a particle size of 0.6-1.0 mm was selected, and diatom liquid (concentration of 100,000 particles / mL) was added at an amount of 15 wt% based on the total weight of the feed. After thorough stirring, the mixture was air-dried in a cool place for 1-2 hours. Then, the live freshwater shrimp were headed and minced into fresh shrimp paste using a meat grinder. The fresh shrimp paste was mixed with the air-dried feed, and Chlorella powder and Schizochytrium powder were added while stirring. The Chlorella powder (concentration of 1×10 9 / g) was added in an amount of 15 wt%, and Schizochytrium powder (concentration 1×10 9 The amount of the raw material of the raw material is 5wt%, the amount of the fresh shrimp paste is 45wt%, and the mixture is evenly mixed and dried in a cool place for 30 minutes to form the initial feed;

[0075] A feed with a particle size of 0.6-1.0 mm was selected, and a diatom liquid (concentration of 100,000 diatoms / mL) was added at a rate of 10 wt% based on the total weight of the feed. After thorough stirring, the feed was aired in a cool place for 1-2 hours. Then, the live freshwater shrimp were headed and minced into fresh shrimp paste using a meat grinder. The fresh shrimp paste was mixed with the air-dried feed, and Chlorella powder and Schizochytrium powder were mixed while stirring. The fresh shrimp paste, Chlorella powder, and Schizochytrium powder were mixed, wherein the Chlorella powder (concentration of 1×10 9 / g) was added in an amount of 10 wt%, and Schizochytrium powder (concentration 1×10 9 The addition amount of shrimp paste is 3wt% and the addition amount of fresh shrimp paste is 25wt%. After mixing evenly, the mixture is dried in a cool place for 30 minutes to form a medium-term feed.

[0076] (3) Mixed feeding:

[0077] A PVC tube with a diameter of 50 mm is placed on the bait nest of pebbles, and the initial feed after airing in step (2) is added from the other end of the PVC tube. After the feed is transported, the PVC tube is removed. The amount of the initial feed after airing is 2 wt% of the total weight of the front rump catfish in the pond. The feed is fed three times a day, and the remaining feed is discharged from the breeding pond after feeding for 40 minutes;

[0078] After feeding the above feed for 20 days, the feed was changed to the mid-term feed and continued to be fed for 10 days. The feeding method, feeding frequency and feeding amount remained unchanged.

[0079] (4) Full feeding:

[0080] After 45 days of acclimation, diatom liquid, fresh shrimp paste, Chlorella powder and Schizochytrium powder are no longer added. At this time, wild catfish can fully consume compound feed. Before feeding, gently tap the outer wall of the breeding pond to form a feeding signal. Keep sending the signal for 1 minute before each feeding, and then start feeding. After 4-5 weeks of training, the fish will form a conditioned reflex to swim to the feeding point upon hearing the signal, thereby improving feeding efficiency and feed utilization. The specific steps of complete feeding include using a fixed-point feeding method, feeding in multiple bait nests on pebbles to ensure that the fish feed evenly, and following the "slow-fast-slow" rhythm when feeding. Feed a small amount first to attract the fish to gather, and then speed up the feeding. When the fish feeding becomes slow, slowly reduce the feeding amount to avoid water pollution caused by the feed being soaked in water for a long time.

[0081] After 100 days of complete feeding, the number of surviving wild catfish was recorded. According to the survival rate = the number of surviving wild catfish after domestication / the total number of wild catfish participating in the domestication × 100%, it was calculated that the survival rate of wild catfish using the domestication method provided in this embodiment was 80%.

[0082] Example 3

[0083] This embodiment provides a method for taming wild catfish, and the specific steps and methods are as follows:

[0084] (1) Food induction:

[0085] The pebbles with microalgae attached prepared in Preparation Example 1 were transferred into the culturing pond of the anterior rump catfish (2 m in diameter) and the amount of pebbles with microalgae attached added was 3 pebbles / m 2 , placed side by side, and after the attached microalgae were scraped off by the wild catfish, pebbles with attached microalgae were added, and this process lasted for 15 days.

[0086] (2) Preparation of feed:

[0087] A feed with a particle size of 0.6-1.0 mm was selected, and diatom liquid (concentration of 100,000 particles / mL) was added at an amount of 15 wt% based on the total weight of the feed. After thorough stirring, the mixture was air-dried in a cool place for 1-2 hours. Then, the live freshwater shrimp were headed and minced into fresh shrimp paste using a meat grinder. The fresh shrimp paste was mixed with the air-dried feed, and Chlorella powder and Schizochytrium powder were added while stirring. The Chlorella powder (concentration of 1×10 8 / g) was added in an amount of 20 wt%, and Schizochytrium powder (concentration 1×10 8 The amount of the raw material of the raw material is 5wt%, the amount of the fresh shrimp paste is 45wt%, and the mixture is evenly mixed and dried in a cool place for 30 minutes to form the initial feed;

[0088] A feed with a particle size of 0.6-1.0 mm was selected, and a diatom liquid (concentration of 100,000 diatoms / mL) was added at a rate of 10 wt% based on the total weight of the feed. After thorough stirring, the feed was aired in a cool place for 1-2 hours. Then, the live freshwater shrimp were headed and minced into fresh shrimp paste using a meat grinder. The fresh shrimp paste was mixed with the air-dried feed, and Chlorella powder and Schizochytrium powder were mixed while stirring. The fresh shrimp paste, Chlorella powder, and Schizochytrium powder were mixed, wherein the Chlorella powder (concentration of 1×10 9 / g) was added in an amount of 15 wt%, and Schizochytrium powder (concentration 1×10 9 The addition amount of shrimp paste is 3wt% and the addition amount of fresh shrimp paste is 25wt%. After mixing evenly, the mixture is dried in a cool place for 30 minutes to form a medium-term feed.

[0089] (3) Mixed feeding:

[0090] A PVC tube with a diameter of 50 mm is placed on the bait nest of pebbles, and the initial feed after airing in step (2) is added from the other end of the PVC tube. After the feed is transported, the PVC tube is removed. The amount of the initial feed after airing is 3 wt% of the total weight of the front rump catfish in the pond. The feed is fed 4 times a day, and the remaining feed is discharged from the culture pond after 40 minutes of feeding.

[0091] After feeding the above feed for 20 days, the feed was changed to the mid-term feed and continued to be fed for 10 days. The feeding method, feeding frequency and feeding amount remained unchanged.

[0092] (4) Full feeding:

[0093] After 45 days of acclimation, diatom liquid, fresh shrimp paste, Chlorella powder and Schizochytrium powder are no longer added. At this time, wild catfish can fully consume compound feed. Before feeding, gently tap the outer wall of the breeding pond to form a feeding signal. Keep sending the signal for 1 minute before each feeding, and then start feeding. After 4-5 weeks of training, the fish will form a conditioned reflex to swim to the feeding point upon hearing the signal, thereby improving feeding efficiency and feed utilization. The specific steps of complete feeding include using a fixed-point feeding method, feeding in multiple bait nests on pebbles to ensure that the fish feed evenly, and following the "slow-fast-slow" rhythm when feeding. Feed a small amount first to attract the fish to gather, and then speed up the feeding. When the fish feeding becomes slow, slowly reduce the feeding amount to avoid water pollution caused by the feed being soaked in water for a long time.

[0094] After 100 days of complete feeding, the number of surviving wild catfish was recorded. According to the survival rate = the number of surviving wild catfish after domestication / the total number of wild catfish participating in the domestication × 100%, it was calculated that the survival rate of wild catfish using the domestication method provided in this embodiment was 78%.

[0095] Example 4

[0096] This embodiment provides a method for taming wild catfish. The specific steps and methods are the same as those in Example 1, except that the pebbles with microalgae attached added to the catfish culture pond are those prepared in Preparation Example 2.

[0097] After 100 days of complete feeding, the number of surviving wild catfish was recorded. According to the survival rate = the number of surviving wild catfish after domestication / the total number of wild catfish participating in the domestication × 100%, it was calculated that the survival rate of wild catfish using the domestication method provided in this embodiment was 70%.

[0098] Example 5

[0099] This embodiment provides a method for taming wild catfish. The specific steps and methods are the same as those in Example 1, except that the pebbles with microalgae attached added to the catfish culture pond are prepared in Preparation Example 3.

[0100] After 100 days of complete feeding, the number of surviving wild catfish was recorded. According to the formula: survival rate = number of surviving wild catfish after domestication / total number of wild catfish participating in domestication × 100%, it was calculated that the survival rate of wild catfish using the domestication method provided in this embodiment was 83%.

[0101] Comparative Example

[0102] This comparative example provides a method for taming wild catfish, and the specific steps and methods are the same as those in Example 1, except that step (1) is not provided, that is, the wild catfish is directly tamed with initial feed and mid-term feed, and then fully fed with feed.

[0103] During the initial feeding process, most wild catfish hardly ate anything. After 15 days of initial feeding, 5 days of intermediate feeding, and 100 days of complete feeding, the survival rate of the wild catfish was finally 20%.

[0104] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A method for taming wild catfish, characterized in that: Pebbles with attached microalgae were used to simulate the feeding environment of wild catfish.

2. The method for taming wild catfish according to claim 1, characterized in that: The method for preparing pebbles with attached microalgae comprises the following steps: Propagate microalgae to form microalgae liquid, soak the pebbles in the microalgae liquid, The microalgae include at least one of chlorella, diatoms, and cyanobacteria.

3. The method for taming wild catfish according to claim 2, characterized in that: The microalgae liquid also includes feed, microbial agents and complex vitamin preparations; and / or, The weight of the pebbles is 4-5 kg / piece, and bait nests with a diameter of 5-8 cm are arranged on the surface of the pebbles, and the number of the bait nests is 1-3.

4. The method for taming wild catfish according to claim 3, characterized in that: The specific steps of propagating microalgae to form microalgae liquid include: in the presence of feed, mixing microalgae, microbial agents and multivitamin preparations to form microalgae liquid, the cultivation time is 3-5 days, and the amount of microalgae added is 1000-1500mL / m 3 The amount of microbial agent added is 2000-3000g / m 3 The addition amount of multivitamin preparation is 600-800g / m 3 and / or, The microbial agent includes at least one of Bacillus subtilis or Bacillus licheniformis.

5. The method for taming wild catfish according to claim 4, characterized in that: During the process of microalgae reproduction to form microalgae liquid, the feed is supplemented every 10-15 days; and / or, While the microalgae liquid is circulating, the pebbles are immersed in the microalgae liquid at a circulation flow rate of 0.05-0.1 m / s and the soaking time is 10-15 days.

6. The method for taming wild catfish according to any one of claims 1 to 5, characterized in that: The method for taming wild catfish comprises the following steps: S1. Using pebbles with microalgae attached to lure wild catfish to feed; S2. feeding the wild catfish with an initial feed comprising 10-15 wt% diatom liquid, 40-45 wt% fresh shrimp paste, 15-20 wt% Chlorella vulgaris, and 5-10 wt% Schizochytrium truncatum; S3, feeding the wild catfish with a mid-term feed, wherein the mid-term feed includes 5-10 wt% diatom liquid, 20-25 wt% fresh shrimp paste, 10-15 wt% Chlorella vulgaris and 3-5 wt% Schizochytrium; S4. Use feed to tame wild catfish.

7. The method for taming wild catfish according to claim 6, characterized in that: The feeding period in step S1 is 15-20 days; and / or, The initial feed in step S2 is fed for 15-20 days; and / or, The feeding time of the mid-term feed in step S3 is 5-10 days; and / or, The feeding frequency of the feed in step S2 and / or step S3 is 3-4 times / day, and the feeding amount of the feed is 2-3 wt% based on the total weight of the trained catfish.

8. The method for taming wild catfish according to claim 6, characterized in that: The concentration of the diatom solution in step S2 and / or step S3 is 100,000-150,000 cells / mL, and the concentration of Chlorella is 1×10 8 -1×10 10 / g, the concentration of Schizochytrium was 1×10 8 -1×10 10 pcs / g.

9. The method for taming wild catfish according to claim 6, characterized in that: In the step S4, before feeding the feed, the outer wall of the culture pond is knocked for at least 1 minute before feeding the feed; and / or, The amount of pebbles with microalgae attached added in step S1 is 3-5 pebbles / m 2 .

10. The method for taming wild catfish according to claim 6, characterized in that: In step S4, the wild catfish is tamed by feeding at fixed points.

Images