Essential oil composition for relieving allergic rhinitis and preparation method and application thereof

Abstract

The invention provides an essential oil composition for relieving allergic rhinitis as well as a preparation method and application thereof, and belongs to the technical field of essential oil compositions. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1-9 parts of pericarpium citri reticulatae, 1-9 parts of fresh ginger, 1-9 parts of rhizoma atractylodis macrocephalae, 1-9 parts of mint and 1-9 parts of Roman chamomile. The essential oil composition disclosed by the invention can be used for effectively relieving symptoms such as rhinorrhea, nasal obstruction and sneezing of allergic rhinitis by tonifying qi, activating spleen, clearing damp and dredging orifices. Experimental results show that the traditional Chinese medicine compound essential oil can reduce rhinitis symptoms and reduce nasal mucosa inflammation, and a convenient, quick-acting and side-effect-free supplementary treatment method is provided for rhinitis patients.

Description

Technical Field

[0001] This invention belongs to the field of essential oil composition technology, and particularly relates to an essential oil composition for relieving allergic rhinitis, its preparation method and application. Background Technology

[0002] Allergic rhinitis (AR) is a common chronic disease characterized by allergic reactions to allergens such as dust and pollen, resulting in symptoms including sneezing, runny nose, and nasal congestion. It is often accompanied by other inflammations such as asthma and allergic conjunctivitis. The prevalence of AR is increasing. Allergic rhinitis symptoms can have long-term impacts on patients' learning, work, and interpersonal relationships.

[0003] Antihistamines and intranasal corticosteroids are common treatment options for allergic rhinitis (AR). Since allergic rhinitis cannot be completely cured, long-term adherence to the treatment plan is crucial for effective management. However, many patients have low satisfaction and compliance with long-term treatment, and some antihistamines have drowsiness as a side effect, further impacting patients' lives. Many patients are still seeking new treatments, especially those suitable for long-term use. According to traditional Chinese medicine theory, allergic rhinitis originates from the invasion of external cold combined with insufficient Yang energy, weak spleen and stomach, and imbalance of the lungs and internal organs. Chinese herbal medicine can directly relieve the symptoms of allergic rhinitis and comprehensively regulate the lungs and internal organs, warming and replenishing Yang energy, thus treating allergic rhinitis through long-term conditioning of the body. Aromatherapy, in particular, uses herbal essential oils to directly act on the nasal mucosa, offering higher efficacy than oral medications. It is significantly effective in reducing nasal allergy symptoms and is convenient to use, potentially supplementing or replacing current treatments for allergic rhinitis. Summary of the Invention

[0004] In view of this, one of the objectives of the present invention is to provide an essential oil composition for relieving allergic rhinitis.

[0005] A second objective of this invention is to provide a method for preparing the essential oil composition.

[0006] A third objective of this invention is to provide the application of the essential oil composition or the preparation method in the preparation of drugs for relieving or treating allergic rhinitis.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0008] An essential oil composition for relieving allergic rhinitis, comprising the following ingredients by weight: 1-9 parts dried tangerine peel, 1-9 parts fresh ginger, 1-9 parts Atractylodes macrocephala, 1-9 parts peppermint, and 1-9 parts Roman chamomile.

[0009] The present invention also provides a method for preparing the traditional Chinese medicine composition, comprising the following steps:

[0010] Tangerine peel, peppermint, and Roman chamomile were extracted using steam distillation to obtain tangerine peel essential oil, peppermint essential oil, and chamomile essential oil, respectively.

[0011] Ginger essential oil and Atractylodes macrocephala essential oil were obtained by CO2 supercritical fluid extraction, respectively.

[0012] The essential oils obtained from the extraction of each raw material are refined using a sodium chloride solution;

[0013] The refined essential oils are mixed to obtain mixture 1, which is then mixed with sweet almond oil.

[0014] Preferably, when using steam distillation for extraction, the mass-to-volume ratio of the Chinese herbal medicine to water is 1g:(25-35)mL.

[0015] Preferably, the extraction temperature of the steam distillation method is 95-100℃, and the extraction time is 1-5 hours.

[0016] Preferably, when using the supercritical CO2 fluid extraction method, the temperature of the supercritical CO2 fluid is 31–50°C, and the extraction pressure is 7.5–30 MPa.

[0017] Preferably, the mass fraction of the sodium chloride solution is 1.5% to 2%.

[0018] Preferably, the volume ratio of mixture 1 to sweet almond oil is 1:3.

[0019] The present invention also provides the use of the essential oil composition or the preparation method described herein in the preparation of a medicament for relieving and / or treating allergic rhinitis.

[0020] Preferably, the essential oil is applied directly to the skin around the nose.

[0021] Preferably, the essential oil is placed in an essential oil diffuser for diffusion.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] This invention provides an essential oil composition for relieving allergic rhinitis. The formula is clearly defined, with tangerine peel and atractylodes macrocephala as the principal herbs to improve the constitution of rhinitis patients who suffer from spleen and stomach weakness and dampness accumulation; peppermint as the assistant herb to dispel wind and clear the nasal passages, relieving rhinitis symptoms; ginger as the adjuvant herb to warm the middle and dispel cold, mitigating the pungent and cooling properties of peppermint and enhancing its effects of dispelling cold and dampness; and Roman chamomile as the guiding herb to alleviate mood and sleep problems caused by rhinitis. The entire formula, through tonifying qi and strengthening the spleen, dispelling dampness and clearing the nasal passages, is primarily used to treat symptoms such as sneezing, runny nose, and nasal congestion caused by allergic rhinitis. This traditional Chinese medicine compound essential oil shows potential efficacy in relieving allergic rhinitis symptoms.

[0024] This invention utilizes the properties of various medicinal herbs to extract essential oils from each herb, employing aromatherapy to treat allergic rhinitis (AR). The compound essential oils (EOs) of this invention have shown potential efficacy in alleviating AR. In experiments, the provided EOs reduced sneezing, runny nose, and nose-wiping symptoms in mice. EOs also reduced nasal inflammation and nasal mucosal thickness. This provides evidence that compound essential oils of traditional Chinese medicine can serve as an effective complementary treatment for AR. Attached Figure Description

[0025] Figure 1 The effect of compound essential oils of traditional Chinese medicine on the symptoms of allergic rhinitis in mice was investigated. * represents p<0.05; ** represents p<0.01.

[0026] Figure 2 Statistical analysis of mouse nose-scratching data, where * represents p<0.05; ** represents p<0.01; *** represents p<0.001; **** represents p<0.0001;

[0027] Figure 3 Statistical analysis of sneezing data in mice, where * represents p<0.05; ** represents p<0.01; *** represents p<0.001; **** represents p<0.0001;

[0028] Figure 4 ELISA analysis of INF-γ concentration in mouse nasal lavage fluid;

[0029] Figure 5 The effect of essential oils on nasal mucosal inflammation in mice;

[0030] Figure 6 The effect of essential oils on the thickness of nasal mucosa in mice. Detailed Implementation

[0031] This invention provides an essential oil composition for relieving allergic rhinitis, comprising the following ingredients by weight: 1-9 parts dried tangerine peel, 1-9 parts fresh ginger, 1-9 parts Atractylodes macrocephala, 1-9 parts peppermint, and 1-9 parts Roman chamomile.

[0032] In this invention, the preferred amount of dried tangerine peel is 3-6 parts, more preferably 5 parts; the preferred amount of ginger is 3-6 parts, more preferably 5 parts; the preferred amount of Atractylodes macrocephala is 3-6 parts, more preferably 5 parts; the preferred amount of peppermint is 3-6 parts, more preferably 5 parts; and the preferred amount of Roman chamomile is 3-6 parts, more preferably 5 parts. This invention does not have any special limitations on the specific source of the above-mentioned raw materials; conventional commercially available products in the art can be used.

[0033] In this invention, tangerine peel (Chenpi) is the dried, mature pericarp of Citrus reticulata Blanco and its cultivated varieties, belonging to the Rutaceae family. It is bitter and pungent in taste, and warm in nature; it enters the lung and spleen meridians. It has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm; it is mainly used to treat abdominal distension, poor appetite, vomiting and diarrhea, and cough with excessive phlegm. Atractylodes macrocephala (Baizhu) is the dried rhizome of Atractylodes macrocephala, belonging to the Asteraceae family. It is bitter and sweet in taste, and warm in nature; it enters the spleen and stomach meridians. It has the effects of removing dampness and promoting dryness, harmonizing the middle jiao and replenishing qi, and strengthening the spleen and stomach; it is mainly used to treat spleen and stomach weakness, loss of appetite, fatigue, shortness of breath, abdominal distension, diarrhea, and phlegm retention. Tangerine peel and Atractylodes macrocephala are used together as the principal herbs to improve the constitution of rhinitis patients with spleen and stomach weakness and dampness obstruction, thus treating the main symptoms of rhinitis.

[0034] In this invention, peppermint refers to the dried aerial parts of *Mentha haplocalyx* Briq., a plant belonging to the genus *Mentha* of the Lamiaceae family. It has a pungent taste and cool properties; it enters the lung and liver meridians. It has the effects of dispersing wind-heat, clearing the head and eyes, relieving sore throat and promoting rash eruption, soothing the liver and regulating qi, and resolving dampness and harmonizing the middle jiao. It is mainly used to treat wind-heat colds, early stages of wind-heat syndrome, headache, red eyes, and sore throat. Peppermint is an assistant herb, having the effect of dispelling wind and clearing the nasal passages, enhancing the efficacy of the principal herb in relieving rhinitis symptoms.

[0035] In this invention, ginger refers to the fresh rhizome of *Zingiber officinale*, a plant in the ginger family (Zingiberaceae). It has a pungent taste and slightly warm properties; it enters the lung, spleen, and stomach meridians. It has the effects of relieving exterior syndromes and dispelling cold, warming the middle jiao and stopping vomiting, warming the lungs and stopping cough, and detoxifying; it is mainly used to treat colds due to wind-cold, spleen and stomach cold syndrome, vomiting due to stomach cold, and cough due to lung cold. Ginger is used as an adjuvant to warm the middle jiao and dispel cold, mitigating the pungent and cooling properties of mint, and enhancing the overall effect of the formula in dispelling cold and dampness.

[0036] In this invention, Roman chamomile refers to the dried flowers of *Chamaemelumnobile*, a species belonging to the genus *Chamaemelumnobile* in the family Asteraceae. Chamomile has a slightly bitter, sweet, and fragrant taste, and enters the liver, kidney, and lung meridians. It has the effects of calming the mind, relieving allergies, improving digestion, and promoting sleep and relieving pain. Roman chamomile is used as the guiding herb in the formula, possessing anti-inflammatory and mood-soothing effects, and alleviating mood and sleep problems caused by rhinitis.

[0037] The essential oil composition of the present invention has a clear formulation and treats and relieves the symptoms of allergic rhinitis by replenishing qi and strengthening the spleen, removing dampness and clearing the orifices.

[0038] The present invention also provides a method for preparing the traditional Chinese medicine composition, comprising the following steps: tangerine peel, peppermint, and chamomile are extracted by steam distillation to obtain tangerine peel essential oil, peppermint essential oil, and chamomile essential oil, respectively; ginger and atractylodes are extracted by supercritical CO2 fluid extraction to obtain ginger essential oil and atractylodes essential oil, respectively; the essential oils obtained from the extraction of each raw material are refined with sodium chloride solution; the refined essential oils are mixed to obtain mixture 1, and mixture 1 is mixed with sweet almond oil.

[0039] In this invention, when using steam distillation for extraction, the preferred mass-to-volume ratio of the herb to water is 1g:(25-35)mL, more preferably 1g:(28-32)mL, and even more preferably 1g:30mL. The preferred extraction temperature using steam distillation is 95-100℃, more preferably 98℃; the preferred extraction time is 1-5h, more preferably 2-4h, and even more preferably 3h.

[0040] In this invention, when using the supercritical CO2 fluid extraction method, the temperature of the supercritical CO2 fluid is preferably 31-50°C, more preferably 35-45°C, and even more preferably 40°C; the extraction pressure is preferably 7.5-30 MPa, more preferably 15-20 MPa.

[0041] In this invention, the essential oils obtained from the extraction of each raw material need to be refined, preferably using a sodium chloride solution. The mass fraction of the sodium chloride solution is preferably 1.5% to 2%, more preferably 1.8%. By adding a sodium chloride solution to the extracted essential oils, the oil-water mixture is fully separated, resulting in essential oils with higher purity.

[0042] In this invention, the volume ratio of mixture 1 to sweet almond oil is preferably 1:3. This invention uses sweet almond oil as a base essential oil, and the essential oil composition of this invention, after being diluted with sweet almond oil, is used to relieve and / or treat allergic rhinitis.

[0043] This invention also provides the use of the essential oil composition or the preparation method described herein in the preparation of a medicament for relieving and / or treating allergic rhinitis. The essential oil composition provided by this invention can reduce nasal inflammation, thereby relieving and / or treating symptoms of allergic rhinitis.

[0044] When using the essential oil composition of the present invention to relieve and / or treat allergic rhinitis, it is preferably administered to the body by inhalation or topical application. When applying topically, it is preferable to apply the essential oil directly to the skin around the nose, preferably to the Yingxiang acupoint, and massage can be used as an aid during application. When inhaling, it is preferable to place the essential oil in an essential oil diffuser for diffusion.

[0045] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0046] Example 1

[0047] An essential oil composition for relieving allergic rhinitis, comprising the following ingredients by weight:

[0048] 5 parts dried tangerine peel, 5 parts ginger, 5 parts Atractylodes macrocephala, 5 parts mint, and 5 parts Roman chamomile.

[0049] The preparation method is as follows:

[0050] Tangerine peel, peppermint, and Roman chamomile essential oil were extracted by steam distillation to obtain tangerine peel essential oil, peppermint essential oil, and Roman chamomile essential oil, respectively.

[0051] Preparation method of tangerine peel essential oil: After tangerine peel is crushed, it is placed in a distillation device, and water is added according to the mass-volume ratio of Chinese medicine to water 1g:32mL. The mixture is heated at 100℃ for 2 hours to extract the aromatic components by allowing water vapor to pass through the plant raw material. The water vapor containing aromatic components is cooled in a condenser to form a liquid. The essential oil and water are separated by an oil-water separator to obtain crude tangerine peel essential oil. 1.8% sodium chloride is added to the crude essential oil, stirred thoroughly, and filtered to obtain tangerine peel essential oil.

[0052] Peppermint and Roman chamomile essential oils are prepared using the same method as tangerine peel essential oil.

[0053] Ginger essential oil and Atractylodes macrocephala essential oil were obtained by CO2 supercritical fluid extraction.

[0054] Preparation method of ginger essential oil: Slice ginger and dry it until the moisture content does not exceed 10%. Crush the dried ginger slices and pass them through a 40-mesh sieve. Put the ginger powder into the extraction vessel of a supercritical CO2 extraction device, adjust the extraction pressure to 30 MPa, the extraction temperature to 50℃, and the extraction time to 110 minutes. During the extraction process, the CO2 flow rate is maintained at 15 L / h. Separate the ginger in a separation vessel at a separation pressure of 5 MPa and a separation temperature of 50℃ to obtain crude ginger essential oil. Add 1.8% sodium chloride to the crude essential oil, stir thoroughly, and filter to obtain ginger essential oil.

[0055] Atractylodes macrocephala essential oil is prepared using the same method as ginger essential oil.

[0056] Mix tangerine peel essential oil, peppermint essential oil, Roman chamomile essential oil, ginger essential oil and atractylodes essential oil to obtain mixture 1. Mixture 1 is then mixed with sweet almond oil in a volume ratio of 1:3 to obtain an essential oil composition.

[0057] Example 2

[0058] An essential oil composition for relieving allergic rhinitis, comprising the following ingredients by weight:

[0059] 8 parts dried tangerine peel, 3 parts ginger, 8 parts Atractylodes macrocephala, 3 parts mint, and 3 parts Roman chamomile.

[0060] The preparation method is the same as in Example 1.

[0061] Example 3

[0062] An essential oil composition for relieving allergic rhinitis, comprising the following ingredients by weight:

[0063] 3 parts dried tangerine peel, 2 parts ginger, 3 parts Atractylodes macrocephala, 1 part mint, and 1 part Roman chamomile.

[0064] The preparation method is the same as in Example 1.

[0065] Example 4

[0066] This embodiment explores the effect of the traditional Chinese medicine compound essential oil obtained in Example 1 on relieving symptoms of allergic rhinitis through the nasal cavity and its effect on reducing nasal inflammation.

[0067] 1. Experimental materials

[0068] (1) SPF-grade BALB / c male mice (purchased from Guangzhou Jinbo Biotechnology Co., Ltd.), weighing 18-20g, were randomly divided into 7 groups with 8 mice in each group according to their weight. The 7 groups were: low-dose group of compound essential oil of traditional Chinese medicine (EOs-L group), medium-dose group of compound essential oil of traditional Chinese medicine (EOs-M group), high-dose group of compound essential oil of traditional Chinese medicine (EOs-H group), nasal drop group of compound essential oil of traditional Chinese medicine, normal control group, AR model group (AR group) and positive control group.

[0069] 2. Experimental Methods

[0070] On days 1, 7, and 15, mice in the AR, EOs-L, EOs-M, and EOs-H groups, as well as the nasal drops of the traditional Chinese medicine compound essential oil and the positive control, were sensitized by intraperitoneal injection of 200 μL of PBS containing 50 μg OVA (ovalbumin) and 2 mg aluminum hydroxide. On days 21, 23, 25, and 27, allergic rhinitis was induced in mice by nasal drops of 20 μL of PBS containing 400 μg OVA. The normal control group mice were simultaneously treated with the same volume of PBS.

[0071] Mixture 1, obtained by mixing tangerine peel essential oil, peppermint essential oil, Roman chamomile essential oil, ginger essential oil, and atractylodes macrocephala essential oil in Example 1, was mixed with sweet almond oil at volume ratios of 4:6, 6:4, and 1:0 to prepare low, medium, and high dose traditional Chinese medicine compound essential oils (EOs-L (low dose) group, EOs-M (medium dose) group, and EOs-H (high dose) group). From days 21 to 27, prior to nasal stimulation, mice were treated with medication: the EOs-L, EOs-M, and EOs-H groups inhaled their corresponding doses of traditional Chinese medicine compound essential oil for 2 hours before nasal stimulation; the nasal stimulation group received 20 μL of the low-dose traditional Chinese medicine compound essential oil via nasal drops; the positive control group received 1 mg / kg dexamethasone via gavage; and the normal control group received 20 μL of PBS. Mice were videotaped for 30 minutes after nasal stimulation, and subsequent behavioral assessments of allergic rhinitis were performed.

[0072] 3. Behavioral observation and assessment

[0073] Scoring criteria were established and recorded based on the severity and timing of nasal itching, sneezing frequency, and runny nose in mice, as shown in Table 1. Each observation lasted 30 minutes. A model was considered successfully established if the scores for all three symptoms reached or exceeded 5 points. The scoring criteria were as follows: Nasal itching – occasional scratching of the nose with a single forelimb: 1 point; scratching the nose with both forelimbs: 2 points; continuous rubbing of the nose in various places: 3 points; Sneezing – 1 to 3 times: 1 point; 4 to 10 times: 2 points; more than 10 times: 3 points; Runny nose – flowing to the anterior nasal cavity: 1 point; flowing beyond the anterior nasal cavity: 2 points; flowing to various parts of the face: 3 points. The total score was recorded using an additive method. The average number of times the mouse scratched its nose and sneezed within 10 minutes was recorded.

[0074] Table 1. Mouse behavioral scoring criteria

[0075]

[0076]

[0077] 4. Animal handling and tissue specimen collection

[0078] On day 27, after recording, the animals were euthanized. Whole blood samples were collected via ocular sampling into anticoagulant tubes. A catheter was inserted into the heart from the left ventricle to the aorta. The right atrium was cut open, and physiological saline and 4% PFA were infused sequentially. The animal was then decapitated to expose the nasal cavity. A syringe containing physiological saline was inserted into the nasopharynx, and 300 μL of physiological saline was injected. Irrigation fluid was collected from the nostrils. Brain and nasal tissues were harvested and immediately fixed in 4% PFA.

[0079] 5. Animal tissue in vitro experiments

[0080] Nasal cavity tissue sectioning and staining: Tissue blocks were sequentially dehydrated in 70%, 80%, 85%, 90%, and 95% ethanol for 30 minutes each, followed by treatment in anhydrous ethanol for 30 minutes, and finally cleared in xylene for 30 minutes. The cleared tissue was then immersed twice in molten paraffin for 60 minutes each time. For embedding, the tissue block was placed in an embedding cassette, molten paraffin was poured in, and the mixture was allowed to cool and solidify. The paraffin block was fixed on a microtome, cut into 5mm thick slices, spread in hot water, mounted on glass slides, and dried in a 45°C oven. The paraffin was removed from the sections with xylene for 5–10 minutes each, followed by dehydration with anhydrous ethanol, 95% ethanol, 80% ethanol, and 70% ethanol for 5 minutes each, and finally rinsed with water for 3–5 minutes. Stain the sections in hematoxylin solution for 5 minutes, separate them with 0.5% hydrochloric acid alcohol for 10 seconds, invert them with saturated disodium hydrogen phosphate solution for 10 minutes, dehydrate them successively with 70% ethanol and 80% ethanol for 5 minutes each, and stain them with eosin for 30 seconds. After dehydration with anhydrous ethanol, clear the sections in xylene, add resin, and seal with a coverslip.

[0081] ELISA: Centrifuge the nasal irrigation fluid sample to remove cell debris and impurities, and collect the supernatant. Add 100 μl of sample or standard to each well of a 96-well microplate pre-coated with anti-IFN-γ antibody and incubate at room temperature for 2 hours. After washing the plate four times, add 100 μl of biotinylated anti-IFN-γ detection antibody and incubate at room temperature for 1 hour. After washing again, add 100 μl of horseradish peroxidase-labeled streptavidin (HRP-Streptavidin) and incubate at room temperature for 30 minutes. After washing the plate four times, add 100 μl of TMB chromogenic solution and incubate in the dark for 10 minutes until color development. Add 50 μl of stop solution to change the color from blue to yellow, and read the absorbance value at 450 nm using a microplate reader. Calculate the concentration of IFN-γ in the sample based on the standard curve.

[0082] 6. Experimental Results

[0083] (1) The effect of compound essential oils of traditional Chinese medicine on the symptoms of allergic rhinitis

[0084] This invention measured the relief of rhinitis symptoms after OVA rhinitis modeling in mice. From Figure 1 As can be seen from the measurement criteria in Table 1, the AR model group mice showed improved rhinitis symptoms after modeling, indicating successful rhinitis modeling. In contrast, the positive control group and the essential oil group mice showed improved rhinitis symptoms. Figure 2 and Figure 3 As can be seen, compared with the model group, the positive control group and the essential oil group mice showed significantly reduced nose-scratching and sneezing behaviors. The data indicate that essential oils are effective in relieving symptoms of allergic rhinitis.

[0085] (2) Improvement of nasal mucosal inflammation by compound essential oils made from traditional Chinese medicine

[0086] This invention measured the effect of OVA-induced rhinitis modeling on the nasal mucosa in mice. Figure 4 It can be seen that the concentration of IFN-γ increased in the nasal cavity of AR model mice after modeling. In contrast, the concentration of IFN-γ decreased in the experimental group and the positive control group. The data indicate that essential oils can reduce nasal inflammatory factors caused by allergic rhinitis.

[0087] from Figure 5 It can be seen that the nasal mucosa of the AR model group mice was significantly swollen after modeling. Figure 6 It can be seen that the nasal cavity thickness of mice in the AR model group increased. In contrast, the nasal mucosal thickness of the experimental group and the positive control group decreased. The data indicate that essential oils can reduce nasal mucosal inflammation caused by allergic rhinitis.

[0088] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An essential oil composition for relieving allergic rhinitis, characterized in that, By weight, it includes the following ingredients: 1-9 parts dried tangerine peel, 1-9 parts fresh ginger, 1-9 parts Atractylodes macrocephala, 1-9 parts mint, and 1-9 parts Roman chamomile.

2. The method for preparing the traditional Chinese medicine composition according to claim 1, characterized in that, Includes the following steps: Tangerine peel, peppermint, and chamomile were extracted using steam distillation to obtain tangerine peel essential oil, peppermint essential oil, and chamomile essential oil, respectively. Ginger essential oil and Atractylodes macrocephala essential oil were obtained by CO2 supercritical fluid extraction, respectively. The essential oils obtained from the extraction of each raw material are refined using a sodium chloride solution; The refined essential oils are mixed to obtain mixture 1, which is then mixed with sweet almond oil.

3. The preparation method according to claim 2, characterized in that, When using steam distillation for extraction, the mass-to-volume ratio of the Chinese herbal medicine to water is 1g:(25-35)mL.

4. The preparation method according to claim 2, characterized in that, The extraction temperature for steam distillation is 95–100℃, and the extraction time is 1–5 hours.

5. The preparation method according to claim 2, characterized in that, When using the supercritical CO2 fluid extraction method, the temperature of the supercritical CO2 fluid is 31–50℃, and the extraction pressure is 7.5–30 MPa.

6. The preparation method according to claim 2, characterized in that, The sodium chloride solution has a mass fraction of 1.5% to 2%.

7. The preparation method according to claim 2, characterized in that, The volume ratio of mixture 1 to sweet almond oil is 1:

3.

8. The use of the essential oil composition of claim 1 or the preparation method of any one of claims 2 to 7 in the preparation of a drug for relieving and / or treating allergic rhinitis.

9. The application according to claim 8, characterized in that, Apply the essential oil directly to the skin around the nose.

10. The application according to claim 8, characterized in that, Place the essential oil in an essential oil diffuser to diffuse it.

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