Antibacterial antioxidant multifunctional composite scaffold material and preparation method thereof

Abstract

The invention discloses an antibacterial and antioxidant multifunctional composite scaffold material and a preparation method thereof, the scaffold material is a gelatin-polylactic acid composite scaffold containing functional particles, the functional particles are curcumin-coated manganese ferrite particles, and arginine is loaded on a coating layer of the functional particles. According to the invention, the antibacterial effect of Mn < 2 + > released by the functional particles is utilized, and the excellent antioxidant effect of curcumin and arginine is utilized, so that excessive active oxygen generated by a bone tissue injury part is removed, the purpose of regulating and controlling the microenvironment of a bone injury area is achieved, and bone tissue regeneration is promoted.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medical repair stents, and particularly relates to an antibacterial and antioxidant multifunctional composite stent material and a preparation method thereof. BACKGROUND

[0002] With the development of medical and beauty field, more and more attention and research are paid to tissue regeneration materials capable of promoting cell differentiation and proliferation, inducing tissue regeneration and accelerating tissue repair. As an important part of tissue engineering, a repair stent can provide structural support and promote cell proliferation, differentiation and tissue regeneration. In the repair of bone-cartilage interface damage, a structural gradient functional repair stent can be constructed according to the biochemical gradient of bone-cartilage.

[0003] In the repair process, bacterial infection can seriously affect the repair result. After bacteria invade the bone defect site, they usually cause a strong inflammatory response, and immune cells such as neutrophils and macrophages release a large amount of reactive oxygen species (ROS) to resist pathogens. Moderate ROS helps to kill bacteria and promote local immune defense, but excessive accumulation of ROS not only inhibits the proliferation and differentiation of bone cells, but also exacerbates local inflammation and disrupts the normal repair process of the tissue. In bone tissue repair, chronic inflammation and ROS elevation caused by infection promote the activity of osteoclastic tissue and accelerate bone resorption, further exacerbating bone defects.

[0004] At present, the biomaterials for constructing repair stents include natural biomaterials such as commonly used gelatin materials, which have excellent hemostatic performance and high processability, but have poor antibacterial activity and cannot meet the demand of preventing bacterial infection, so further improvement is needed.

[0005] The information disclosed in this BACKGROUND section is only intended to increase an understanding of the general context in which the present application can be practiced and is not admitted to be prior art. SUMMARY

[0006] The present application aims to provide an antibacterial and antioxidant multifunctional composite stent material and a preparation method thereof, which has good biocompatibility and can exert antibacterial and antioxidant effects.

[0007] To achieve the above-mentioned purpose, the technical scheme provided by an embodiment of the present application is as follows:

[0008] An antibacterial and antioxidant multifunctional composite stent material, which is a gelatin-polylactic acid composite stent containing functional particles, the functional particles being curcumin-coated manganese ferrite particles, and arginine being loaded on the coating layer of the functional particles.

[0009] In one or more embodiments of the present application, the functional particle has a particle size of 50-70 nm.

[0010] In one or more embodiments of the present application, the mass ratio of curcumin to manganese ferrite is (2-4):7.

[0011] In one or more embodiments of the present application, the arginine has a mass of 8-12% of the mass of the functional particle.

[0012] The technical solution provided by one specific embodiment of the present application is as follows:

[0013] A preparation method of an antibacterial and antioxidant multifunctional composite scaffold material, comprising the following steps:

[0014] S1, adding functional particles, gelatin and polylactic acid into a solvent, stirring to prepare a mixed solution;

[0015] S2, preparing the antibacterial and antioxidant multifunctional composite scaffold material by electrospinning the mixed solution.

[0016] In one or more embodiments of the present application, the mass ratio of gelatin to polylactic acid is (0.4-1.4):1.

[0017] In one or more embodiments of the present application, the mass concentration of the mixed solution is 10-30%.

[0018] In one or more embodiments of the present application, the mass concentration of the functional particles in the mixed solution is 0.05-0.5%.

[0019] In one or more embodiments of the present application, the preparation method of the functional particles is as follows:

[0020] Step 1, mixing an aqueous solution of trivalent iron salt and an aqueous solution of divalent manganese salt, then adding a dispersing agent and a buffer, adjusting the pH value of the solution to 10-12, and heating to react;

[0021] Step 2, adding a curcumin solution to the reaction product in Step 1, heating to react for a certain time, and then centrifuging, washing and drying to obtain manganese ferrite particles coated with curcumin;

[0022] Step 3, dissolving arginine in a buffer, then adding the manganese ferrite particles coated with curcumin, and then performing ultrasonic treatment, magnetic stirring, centrifugation, washing, and freeze-drying to obtain functional particles.

[0023] In Step 1 of the present application, the aqueous solution of trivalent iron salt and the aqueous solution of divalent manganese salt are mixed at 50-80℃, the heating reaction temperature is 180-220℃, and the reaction time is 12-15h. In Step 2, the heating reaction temperature is 80-100℃, and the reaction time is 1.5-3h.

[0024] Compared with the prior art, the antibacterial and antioxidant multifunctional composite stent material in the application has excellent biocompatibility, and the functional particles carried in the stent can effectively release Mn 2+ , so that the stent has certain antibacterial effect. Meanwhile, after the manganese ferrite particles are wrapped with curcumin, the particles are more uniformly dispersed in the stent, and the stent has certain antioxidant function. Finally, arginine is loaded on the particles, so that the stent has excellent antioxidant function and can reduce the influence of inflammation induced by bacterial infection. BRIEF DESCRIPTION OF DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0026] Figure 1 It is a transmission electron microscope image of the functional particles in Example 1 of the present application;

[0027] Figure 2 It is a micro-morphology image of the antibacterial and antioxidant multifunctional composite stent material in Example 1 of the present application;

[0028] Figure 3 It is the antioxidant test result of the antibacterial and antioxidant multifunctional composite stent material in Example 1 of the present application;

[0029] Figure 4 It is the antioxidant test result of the composite stent in Comparative Example 1 of the present application;

[0030] Figure 5 It is the antibacterial test result of the antibacterial and antioxidant multifunctional composite stent material in Example 1 of the present application;

[0031] Figure 6 It is the antibacterial test result of the composite stent in Comparative Example 1 of the present application. DETAILED DESCRIPTION

[0032] In order to make the person skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor should belong to the scope of protection of the present application.

[0033] Manganese ferrite (MnFe2O4) exhibits good biological activity in antibacterial, promoting cell proliferation and differentiation, magnetic hyperthermia effect, and as a drug delivery carrier. Its antibacterial effect is achieved by releasing Mn 2+ which can destroy bacterial cell membranes and prevent infection; manganese ferrite can promote the osteogenic differentiation of bone marrow mesenchymal stem cells and promote bone tissue repair; its magnetic hyperthermia effect can kill tumor cells by local heating in tumor treatment; in drug delivery systems, manganese ferrite can help achieve targeted therapy.

[0034] Curcumin is a natural polyphenolic compound with excellent antioxidant properties, which can directly scavenge free radicals in the body, reduce oxidative stress, and also enhance the antioxidant capacity of cells by activating the expression of antioxidant enzymes such as glutathione peroxidase and superoxide dismutase. In addition, curcumin can inhibit the activity of enzymes related to free radical generation, such as lipoxygenase and cyclooxygenase, thereby reducing the excessive generation of free radicals. These antioxidant properties make curcumin have wide application potential in preventing and treating chronic diseases, slowing down the aging process, and protecting cardiovascular health.

[0035] Arginine indirectly scavenges reactive oxygen species through its conversion to nitric oxide in the body, and nitric oxide as a signaling molecule can regulate oxidative stress, reduce the excessive generation of reactive oxygen species, and protect cells from oxidative damage. In addition, arginine can further promote the removal of reactive oxygen species by enhancing the activity of the antioxidant enzyme system, thereby reducing the level of oxidative stress in cells and helping to maintain the health status of cells and tissue function, especially in the cardiovascular system and immune system.

[0036] The present application applies manganese ferrite, curcumin and arginine to the stent, so that the stent can not only exert antibacterial effect, but also reduce the excessive accumulation of reactive oxygen species through antioxidant effect, ultimately reducing the impact of inflammation induced by bacterial infection.

[0037] The specific embodiment of the present application provides an antibacterial and antioxidant multifunctional composite stent material, which is a gelatin-polylactic acid composite stent containing functional particles, the functional particles are manganese ferrite particles coated with curcumin, and the functional particles are loaded with arginine on the coating layer.

[0038] Specifically, polylactic acid has good biocompatibility, high mechanical strength and biodegradability, and the composite stent made of polylactic acid and gelatin can improve the biocompatibility and mechanical strength of the stent.

[0039] The functional particles have a nanoscale particle size, specifically 50-70 nm, and the nanoscale particle size is small and has a large specific surface area, which is conducive to cell adhesion and proliferation, and the large specific surface area is also conducive to loading active substances.

[0040] The mass ratio of curcumin to manganese ferrite is (2-4):7, and the mass of arginine is 8-12% of the mass of the functional particles. The curcumin is used to coat the manganese ferrite, which helps the particles to be uniformly dispersed in the stent, and prevents the manganese ferrite from being oxidized due to its large specific surface energy, so that the manganese ferrite can effectively release antibacterial effect. Moreover, the curcumin coated manganese ferrite can be functionalized, which helps to load more active substances. In addition, loading arginine on the curcumin coated manganese ferrite particles can further improve the antioxidant function of the stent. By limiting the mass ratio between curcumin and manganese ferrite and the mass ratio of arginine in the functional particles, the functional particles added to the composite stent can better play the antibacterial and antioxidant effects.

[0041] Another specific embodiment of the present application provides a preparation method of an antibacterial and antioxidant multifunctional composite stent material, comprising steps S1-S2.

[0042] In step S1, the functional particles, gelatin and polylactic acid are added to a solvent to prepare a mixed solution by stirring.

[0043] Specifically, the molecular weight of the gelatin is 5-250,000 Da, and the molecular weight of the polylactic acid is 10-150,000 Da. Selecting gelatin and polylactic acid with specific molecular weights is more conducive to subsequent electrospinning. The mass ratio of gelatin to polylactic acid is (0.4-1.4):1. By limiting the amount ratio of gelatin and polylactic acid, the biocompatibility of the stent can be better improved.

[0044] Further, the solvent is selected from trifluoroethanol, dimethyl sulfoxide and N,N-dimethylformamide. The mass concentration of the mixed solution is 10-30%, i.e. the mass concentration of the solute in the mixed solution is 10-30%, and the mass concentration of the functional particles in the mixed solution is 0.05-0.5%.

[0045] In step S2, the mixed solution is prepared into an antibacterial and antioxidant multifunctional composite stent material by electrospinning.

[0046] Specifically, the electrospinning process parameters are as follows: voltage 15-18 kV; spinning liquid flow rate 2-4 mL / h; distance from needle tip to receiver 18-30 cm; speed of drum receiver 100-150 rpm / min; distance of emitter to and fro 25-35 cm; ambient temperature 25-30℃; and ambient humidity 40-60%.

[0047] Further, the preparation method of the functional particles comprises steps 1-3.

[0048] In step 1, an aqueous solution of trivalent iron salt and an aqueous solution of divalent manganese salt are mixed, and then a dispersing agent and a buffer are added to adjust the pH value of the solution to 10-12, and then heated to react.

[0049] Specifically, the ferric salt is selected from ferric chloride, ferric sulfate and ferric nitrate, the manganese salt is selected from manganese chloride, manganese sulfate and manganese nitrate, the dispersing agent is selected from sodium oleate, sulfate and phosphate, and the buffer is selected from ammonia and NaOH. The ferric salt aqueous solution and the manganese salt aqueous solution are mixed at 50-80℃, and then added into a reaction kettle for reaction at 180-220℃ for 12-15h.

[0050] Step 2: adding curcumin solution into the reaction product in step 1, heating for a certain time, centrifuging, washing and drying after reaction to obtain the curcumin-coated manganese ferrite particles.

[0051] Specifically, the curcumin solution is prepared by dissolving curcumin in N,N-dimethylformamide or N-hydroxysuccinimide. The heating reaction temperature is 80-100℃, and the reaction time is 1.5-3h. The centrifugation speed is 8000-15000rap, and the centrifugation time is 10-30min. The washing is performed by using ultrapure water, acetone or anhydrous ethanol, which is beneficial to prevent the negative influence caused by the adsorption of organic molecules on the particles. The vacuum drying time is not less than 48h.

[0052] Step 3: dissolving arginine in a buffer, then adding the curcumin-coated manganese ferrite particles, and performing ultrasonic treatment, magnetic stirring, centrifugation, washing and freeze-drying to obtain the functional particles.

[0053] Specifically, the buffer is phosphate buffer or Tris hydrochloride buffer, and the concentration of the prepared arginine solution is not less than 2.5mg / ml. The centrifugation speed is 15000rap, and the centrifugation time is 20min. The cross washing is performed by using ultrapure water, acetone and anhydrous ethanol to remove unreacted components and organic molecules adsorbed on the surface of the particles. The freeze-drying time is 36-72h. The particle size of the functional particles is less than 100nm, and preferably, the particle size of the functional particles prepared in the present application is 50-70nm.

[0054] The present application will be further described in detail in combination with specific examples.

[0055] The antioxidant test method of the scaffold material in the present application is as follows: according to the extraction standard (ISO 10993-12), the culture medium is added at the extraction ratio (surface area / volume: 6cm 2 / mL) at 37℃ on a shaking table, and the extraction liquid of 48h is used for subsequent experiments. The cells are added at 3×10 4The density of the holes is inoculated in a 12-well plate, and after 24 h of culture in a 37℃ cell incubator, the original culture medium is discarded and replaced with the corresponding scaffold extraction solution for 4 h. Then the culture medium is discarded, washed with sterile PBS, and the DCFH-DA probe is diluted (1:1000) with serum-free culture medium, 500 μL is added to each well, and incubated in a 37℃ incubator for 20 min. Then the fluorescence intensity is observed using a microscope. The stronger the fluorescence, the more ROS in the cells, which is not effectively removed, and vice versa.

[0056] The method for testing the antibacterial property of the scaffold material is as follows: the scaffold is placed in a well plate and then liquid medium is added for pre-culture. 1 mL of diluted bacterial solution is added to each well and cultured with the scaffold in an incubator for 24 h. After 24 h, the culture medium is discarded and the surface of the scaffold is washed with 0.9% NaCl solution, 500 μL of 2.5% glutaraldehyde solution is added to each well, and fixed at room temperature for 4 h. The fixing solution is discarded, and the scaffold is eluted with 30%, 50%, 70%, 90%, 95%, and 100% ethanol solutions, respectively, for 10 min each time. The eluted sample is dried in a 37℃ vacuum drying oven, the surface of the sample is sprayed with gold, and then observed using SEM. The fewer bacteria on the scaffold, the better the antibacterial effect.

[0057] Example 1

[0058] Preparation of functional particles

[0059] A 0.2M concentration FeCl3·6H2O aqueous solution and a 0.1M concentration MnCl2·4H2O aqueous solution are prepared, and 200 ml of the FeCl3·6H2O aqueous solution and 200 ml of the MnCl2·4H2O aqueous solution are mixed in equal volumes at 70℃, and 3 ml of glycerol tristearate is added to prepare solution A. 100 ml of a 4 mol / L NaOH solution is added to solution A to adjust the pH to 12, and is recorded as solution B. Solution B is poured into a reaction kettle and reacted at 200℃ for 12 h to obtain product C, i.e. manganese ferrite nanoparticles.

[0060] After the product is separated, 100 ml of a 10(w / v)% curcumin N,N-dimethylformamide solution is added, heated to 90℃, and then 5 ml of an ammonia water solution is slowly added, and reacted for 1.5 h. Then the product is separated, washed with anhydrous ethanol 3 times, and finally dried in a vacuum for 60 h to obtain product D, i.e. curcumin-coated manganese ferrite nanoparticles, with a mass ratio of curcumin to manganese ferrite of 3:7.

[0061] 50mg arginine was dissolved in 100ml phosphate buffer (1X, pH 7.4) to prepare solution E, curcumin-coated manganese ferrite nanoparticles were added to solution E, then ultrasonic treatment was performed for 20min, and then continuous magnetic stirring was performed for 48h at a stirring speed of 800r / min. After the end of stirring, the precipitate was separated by centrifugation, and the precipitate was washed 5 times with a mixture of alcohol and pure water at a stirring speed of 15000rpm for 20min, and finally freeze-dried for 72h to obtain functional particles. The mass of arginine is 10% of the mass of the functional particles, and the transmission electron microscope image of the functional particles is shown in Figure 1 .

[0062] Preparation of antibacterial and antioxidant multifunctional composite scaffold material

[0063] 0.3g gelatin with a molecular weight of 100,000 Da and 0.3g polylactic acid with a molecular weight of 100,000 Da were added to 3ml dimethyl sulfoxide, and continuous stirring was performed for 6h to prepare a spinning solution with a mass fraction of 20%, 15mg functional particles were added to the spinning solution, and stirring was continued for 6h, and ultrasonic treatment was performed for 30min to eliminate bubbles, and finally the mixed spinning solution was added to a syringe for electrospinning to prepare an antibacterial and antioxidant multifunctional composite scaffold material. The micro-morphology diagram of the antibacterial and antioxidant multifunctional composite scaffold material is shown in Figure 2 .

[0064] The electrospinning process parameters are as follows: the voltage is 18kV; the flow rate of the spinning solution is 3mL / h; the distance from the needle tip to the receiver is 25cm; the rotating speed of the drum receiver is 120rpm / min; the reciprocating distance of the emitter is 30cm; the environmental temperature is 30℃; and the environmental humidity is 57%.

[0065] Comparative Example 1

[0066] 0.3g gelatin with a molecular weight of 100,000 Da and 0.3g polylactic acid with a molecular weight of 100,000 Da were added to 3ml dimethyl sulfoxide, and continuous stirring was performed for 6h to prepare a spinning solution with a mass fraction of 20%, and the mixed spinning solution was added to a syringe for electrospinning to prepare a composite scaffold.

[0067] The electrospinning process parameters are as follows: the voltage is 18kV; the flow rate of the spinning solution is 3mL / h; the distance from the needle tip to the receiver is 25cm; the rotating speed of the drum receiver is 120rpm / min; the reciprocating distance of the emitter is 30cm; the environmental temperature is 30℃; and the environmental humidity is 57%.

[0068] Figures 3-6 The antibacterial test and antioxidant test results of the scaffolds in Example 1 and Comparative Example 1 are shown in Tables 1 and 2, respectively. Figure 3 The number of bacteria on the scaffold in Example 1 is less than Figure 4 The number of bacteria on the scaffold in Example 1 is less than that on the scaffold in Comparative Example 1, indicating that the scaffold in the present application exhibits a better antibacterial effect.Figure 5 Medium fluorescence is relatively Figure 6 Medium fluorescence is relatively weak, indicating that the scaffold in the present application exhibits better antioxidant performance.

[0069] Comparative Example 2

[0070] The difference between this comparative example and Example 1 is that no glyceryl tristearate is added when preparing manganese ferrite nanoparticles, and the result shows that the manganese ferrite nanoparticles prepared are severely aggregated and cannot be dispersed.

[0071] Comparative Example 3

[0072] The difference between this comparative example and Example 1 is that no NaOH solution is added when preparing manganese ferrite nanoparticles, and the result leads to a decrease in the purity of the manganese ferrite nanoparticles prepared and uneven particle size distribution.

[0073] Comparative Example 4

[0074] The difference between this comparative example and Example 1 is that when preparing curcumin-coated manganese ferrite nanoparticles, the temperature is heated to 70°C and the reaction time is 1 h. The result shows that the curcumin encapsulation rate is low, and curcumin self-polymerization occurs, resulting in a decrease in the purity of the curcumin-coated manganese ferrite nanoparticles prepared.

[0075] Comparative Example 5

[0076] The difference between this comparative example and Example 1 is that when loading arginine, the ultrasonic treatment is 10 min and the continuous magnetic stirring is 20 h. The result leads to an insufficient loading of arginine on the curcumin-coated manganese ferrite nanoparticles, resulting in poor antibacterial and antioxidant performance of the scaffold.

[0077] Comparative Example 6

[0078] The difference between this comparative example and Example 1 is that when preparing the antibacterial and antioxidant multifunctional composite scaffold material, the molecular weight of gelatin is 30,000 Da and the molecular weight of polylactic acid is 30,000 Da. The result leads to a too low viscosity of the spinning solution, and the prepared scaffold is uneven in thickness and has many broken parts.

[0079] Comparative Example 7

[0080] The difference between this comparative example and Example 1 is that when preparing the antibacterial and antioxidant multifunctional composite scaffold material, the mass concentration of functional particles in the mixed spinning solution is 0.01%, and the result leads to poor antibacterial and antioxidant performance of the prepared scaffold.

[0081] Comparative Example 8

[0082] The comparative example is different from example 1 in that no functional particles are prepared in advance, 5 mg of manganese ferrite, 5 mg of curcumin and 5 mg of arginine are directly added to the spinning solution, and then electrospinning is carried out to prepare the scaffold. The result leads to the prepared scaffold with poor antioxidant performance and poor antibacterial performance.

[0083] In summary, the present application realizes sustained release of Mn by using curcumin to coat manganese ferrite and simultaneously loading arginine. 2+ The scaffold continuously exerts antibacterial effect, and simultaneously exerts antioxidant effect by means of curcumin and arginine, so as to remove excess active oxygen generated at the damage site, achieve the purpose of regulating the microenvironment of the bone damage area, and promote bone tissue regeneration.

[0084] It is apparent for those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, but can be implemented in other concrete forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be considered in all aspects as illustrative and not restrictive, and the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and range of the equivalent elements of the claims are intended to be embraced in the present application.

[0085] In addition, it should be understood that although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the specification is described in this way only for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that those skilled in the art can understand.

Claims

1. A multifunctional composite scaffold material with antibacterial and antioxidant properties, characterized in that, The scaffold material is a gelatin-polylactic acid composite scaffold containing functional particles, wherein the functional particles are manganese ferrite particles coated with curcumin, and the coating layer of the functional particles is loaded with arginine.

2. The antibacterial and antioxidant multifunctional composite scaffold material according to claim 1, characterized in that, The particle size of the functional particles is 50–70 nm.

3. The antibacterial and antioxidant multifunctional composite scaffold material according to claim 1, characterized in that, The mass ratio of curcumin to manganese ferrite is (2-4):

7.

4. The antibacterial and antioxidant multifunctional composite scaffold material according to claim 1, characterized in that, The arginine content is 8% to 12% of the mass of the functional particles.

5. A method for preparing an antibacterial and antioxidant multifunctional composite scaffold material, characterized in that, Includes the following steps: S1. Add functional particles, gelatin and polylactic acid to a solvent and stir to prepare a mixture; S2. The mixture is electrospinned to produce an antibacterial and antioxidant multifunctional composite scaffold material.

6. The method for preparing the antibacterial and antioxidant multifunctional composite scaffold material according to claim 5, characterized in that, The mass ratio of gelatin to polylactic acid is (0.4–1.4):

1.

7. The method for preparing the antibacterial and antioxidant multifunctional composite scaffold material according to claim 5, characterized in that, The mass concentration of the mixture is 10-30%.

8. The method for preparing the antibacterial and antioxidant multifunctional composite scaffold material according to claim 5, characterized in that, The functional particle mass concentration in the mixture is 0.05% to 0.5%.

9. The method for preparing the antibacterial and antioxidant multifunctional composite scaffold material according to claim 5, characterized in that, The preparation method of the functional particles is as follows: Step 1: Mix the aqueous solutions of ferric salt and manganese salt, then add dispersant and buffer, adjust the pH of the solution to 10-12, and heat to react; Step 2: Add curcumin solution to the reaction product in Step 1, heat the reaction for a certain time, centrifuge, wash and dry after the reaction to obtain curcumin-coated manganese ferrite particles. Step 3: Dissolve arginine in buffer solution, then add curcumin-coated manganese ferrate particles, and sonicate, magnetically stir, centrifuge, wash, and freeze-dry to obtain functional particles.

10. The method for preparing the antibacterial and antioxidant multifunctional composite scaffold material according to claim 9, characterized in that, In step 1, the aqueous solutions of ferric salt and divalent manganese salt are mixed at 50–80°C, the reaction temperature is heated to 180–220°C, and the reaction time is 12–15 h. In step 2, the heating reaction temperature is 80-100℃ and the reaction time is 1.5-3h.

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