Sulfur-oxidizing bacteria screening enrichment nutrient solution, sulfur-oxidizing bacteria enrichment method and deodorization method

Abstract

The invention discloses a sulfur-oxidizing bacterium screening and enriching nutrient solution, a sulfur-oxidizing bacterium enriching method and a deodorizing method. The sulfur-oxidizing bacterium screening and enriching nutrient solution comprises 1.0 g.L <-1 > of K2HPO4, 0.5 g.L <-1 > of NaHCO3, 0.05 g.L <-1 > of NH4Cl, 0.225 g.L <-1 > of Na2S. 9H2O and 0.001 g.L <-1 > of trace elements, wherein the trace elements comprise 0.5 g.L <-1 > of EDTA (Ethylene Diamine Tetraacetic Acid), 0.01 g.L <-1 > of (NH4) 6Mo7O24. 4H2O, 0.04 g.L <-1 > of ZnSO4. 7H2O, 0.03 g.L <-1 > of MnCl2. 7H2O, 0.02 g.L <-1 > of CuSO4. 5H2O The deodorization performance of the municipal sewage treatment plant is improved through synchronous desulfurization and deamination of the acidophilic sulfur-oxidizing bacteria, and compared with a traditional deodorization process, the synchronous desulfurization and deamination process can enrich high-abundance sulfur-oxidizing bacteria in a short time and shows higher ammonia gas and hydrogen sulfide removal capacity.

Description

Technical Field

[0001] This invention belongs to the field of wastewater treatment, and specifically relates to a nutrient solution for screening and enriching sulfur-oxidizing bacteria, a method for enriching sulfur-oxidizing bacteria, and a deodorization method. Background Technology

[0002] The sulfur-oxidizing bacteria enrichment method is mainly used in wastewater treatment plants, especially for treating odorous gases containing hydrogen sulfide and other similar substances. Background technologies include traditional physical, chemical, and biological deodorization methods.

[0003] Sulfur-oxidizing bacteria obtain energy by oxidizing sulfides (such as hydrogen sulfide) to sulfates; this process is known as sulfur oxidation. Background techniques involve research on the metabolic pathways, growth conditions, and environmental adaptability of sulfur-oxidizing bacteria.

[0004] The activated sludge process is a widely used biological treatment technology for removing organic matter from wastewater. The inoculum sludge used in sulfur-oxidizing bacteria enrichment methods typically originates from A... 2 / O treatment unit aerobic tank activated sludge. This activated sludge contains a large number of microbial communities with high microbial diversity, providing a rich microbial source for the enrichment of sulfur-oxidizing bacteria; it also helps maintain the activity and stability of microorganisms during subsequent enrichment and acclimatization processes. Among them, the microorganisms in the aerobic tank have a highly efficient organic matter degradation capacity, which is very important for the enrichment and activity measurement of sulfur-oxidizing bacteria, because these microorganisms can quickly convert sulfides into sulfates, thereby improving sulfur oxidation efficiency; its reuse helps to achieve resource recycling and reduce waste generation.

[0005] A biofilter (BTF) is a bioreactor used to treat odorous gases produced in wastewater treatment plants. After enrichment and cultivation, activated sludge is inoculated into a sludge-mixed packing material to allow biofilm formation. The packing material is then placed in the BTF for cultivation. Sulfur-oxidizing bacteria exist in the BTF in the form of a biofilm, efficiently removing sulfides.

[0006] Simultaneous desulfurization, deammoniation, and biological deodorization can be used to solve the problem of odor treatment containing hydrogen sulfide and ammonia. Traditional deodorization methods typically treat hydrogen sulfide and ammonia separately, which not only increases the difficulty of treatment but also raises costs. Therefore, simultaneous desulfurization and deammoniation simplifies the process and reduces treatment costs.

[0007] Studies have shown that compared with traditional deodorization processes, simultaneous desulfurization and deamination processes can enrich high-abundance sulfur-oxidizing bacteria in the short term, showing higher potential for sulfide removal. The reason is that the simultaneous desulfurization and deamination process usually uses specific nutrient liquid formulations that are optimized according to the metabolic needs of sulfur-oxidizing bacteria, thereby promoting the growth and reproduction of sulfur-oxidizing bacteria; in this process, by adding sulfide (such as Na2S·9H2O) as a sulfur source, a selective pressure environment is artificially created, which is conducive to the screening and enrichment of microorganisms with sulfur-oxidizing ability; by precisely controlling the operating conditions of the reactor, such as pH, dissolved oxygen (DO), temperature, etc., a more suitable growth environment is provided for sulfur-oxidizing bacteria; in this process, sulfur-oxidizing bacteria are more likely to form biofilms on the surface of the filler, and this biofilm structure is conducive to the attachment and growth of microorganisms, improving their treatment efficiency of sulfide.

[0008] Usually, a continuous cultivation strategy is adopted, which helps to maintain the activity and stability of microorganisms and accelerate the enrichment speed; by regulating the microbial community structure, the simultaneous desulfurization and deamination process can inhibit the growth of non-target microorganisms and induce the pH value of the circulating liquid to enter an acidic state, thereby facilitating the enrichment of target sulfur-oxidizing bacteria; by using inoculated sludge that has been acclimated, the biological reactor can be quickly started up, shortening the time required to reach a high-efficiency desulfurization state; more efficient mass transfer designs, such as optimized filler types and tower structures, are adopted to improve the contact efficiency between gas and liquid, thereby accelerating the enrichment of sulfur-oxidizing bacteria.

[0009] In the simultaneous desulfurization and deamination process, the structure and function of the microbial community play a crucial role. These microbial communities are responsible for oxidizing sulfide to sulfate and converting ammonia nitrogen to nitrogen gas, thereby achieving odor purification. By studying the composition, structure, and function of the microbial community, it is possible to optimize process conditions and improve treatment efficiency.

[0010] The background technology for microbial community analysis includes the use of molecular biology methods (such as 16sRNA sequencing) to analyze and identify the types and community structure of microorganisms involved in sulfur oxidation. SUMMARY

[0011] To address the problems of existing methods, such as the inability to enrich high-abundance sulfur-oxidizing bacteria in the short term, the need for more complex operation procedures and more manual intervention, and the lack of full utilization of the metabolic potential of microorganisms, the present invention proposes a sulfur-oxidizing bacteria screening and enrichment nutrient liquid, a sulfur-oxidizing bacteria enrichment method, and a deodorization method for application in biological deodorization of the influent well of a municipal wastewater treatment plant.

[0012] To achieve the above-mentioned purposes, the technical solutions adopted by the present invention are as follows:

[0013] A sulfur-oxidizing bacteria screening and enrichment nutrient liquid, the nutrient liquid formulation is K2HPO41.0 g·L -1NaHCO3 0.5g·L -1 NH4Cl 0.05g·L -1 Na2S·9H2O 0.225g·L -1 Trace elements 0.001g·L -1 Trace elements composition includes EDTA 0.5g·L -1 (NH4)6Mo7O 24 ·4H2O 0.01g·L -1 ZnSO4·7H2O 0.04g·L -1 MnCl2·7H2O0.03g·L -1 CuSO4·5H2O 0.02g·L -1 CoCl2·6H2O 0.02g·L -1 .

[0014] A sulfur-oxidizing bacteria enrichment method, comprising:

[0015] After the activated sludge from an aerobic tank is pretreated by screening and elutriation, the activated sludge is placed in an upflow reactor, and the activated sludge is continuously supplied with the sulfur-oxidizing bacteria screening and enrichment nutrient solution in claim 1 to screen and enrich sulfur-oxidizing microorganisms.

[0016] Optionally, the height-diameter ratio of the reaction zone of the upflow reactor is 11, and the HRT is 12h.

[0017] Optionally, the activated sludge from the aerobic tank is activated sludge from a sewage treatment plant, and the indexes are as follows: ammonia nitrogen is 0.94mg·L -1 total nitrogen is 0.28g·L -1 total protein is 1.64g·L -1 dissolved protein is 19.23mg·L -1 pH is 7.52, TS is 6.62g·L -1 SS is 4.86g·L -1 VSS is 1.78g·L -1 TCOD is 5.77g·L -1 SCOD is 34.14mg·L -1 .

[0018] A simultaneous desulfurization and deamination biological deodorization method, in which a microbial mixed filler obtained by using the sulfur-oxidizing bacteria enrichment method of any of the present application is used to form a biofilm in a biological trickling filter tower.

[0019] Optionally, the microorganism is Thiomonas_arsenitoxydans, and the relative abundance is 40%.

[0020] Optionally, the biological trickling tower adopts the way of gas-liquid countercurrent and series gas inlet, the chemical washing tower is placed in the rear part of the BTF in the synchronous desulfurization and denitrification mode, the chemical washing tower is placed in the front part of the BTF in the chemical denitrification mode, the circulating liquid enters from the top of the BTF and is discharged from the bottom of the BTF, and the circulating liquid volume update frequency is 50% per day.

[0021] In the aerobic condition, the circulating liquid used in the first to thirtieth day is the sulfur-oxidizing bacteria screening and enrichment nutrient solution of claim 1; in the thirtieth to sixtieth day, the circulating liquid is replaced by tap water, and the circulating liquid pH in the biological trickling tower shows acidity.

[0022] The advantages of the present application are:

[0023] Compared with the prior art, the present application treats the odor of the municipal wastewater treatment plant by enriching sulfur-oxidizing bacteria, and the main advantages are:

[0024] (1) By using specific nutrient solution formula and operating conditions, specific sulfur source is provided for sulfur-oxidizing microorganisms, high-abundance sulfur-oxidizing bacteria can be screened and enriched in a short time, hydrogen sulfide removal efficiency is improved, and ammonia removal can be realized at the same time;

[0025] (2) The biological method is used to remove odor, which reduces the harmful by-products that may be produced by chemical treatment and has less impact on the environment;

[0026] (3) Compared with the chemical treatment method, the biological deodorization method may have lower operation and maintenance costs, especially in long-term operation;

[0027] (4) It can adapt to odor of different concentrations and components, and has good adaptability and flexibility;

[0028] (5) Through the formation of biofilm and the continuous activity of microorganisms, stable and persistent odor removal effect is provided;

[0029] (6) Using activated sludge as inoculated sludge, the sludge has high microbial diversity, which provides a rich source for the enrichment of sulfur-oxidizing bacteria, and is beneficial to improve the enrichment efficiency of sulfur-oxidizing bacteria and realize the recycling of resources;

[0030] (7) The upflow reactor is used for enrichment, and this specific reactor structure and operating parameter setting are more conducive to the full contact of microorganisms and nutrient solution, so that the microorganisms can grow and metabolize better in this environment, thereby improving the enrichment effect of sulfur-oxidizing bacteria;

[0031] (8) It can be applied to wastewater treatment plants of different scales and has good scalability;

[0032] (9) Through long-term domestication and enrichment, a stable and efficient microbial community is established, which helps to maintain the odor treatment effect for a long time. DETAILED DESCRIPTION

[0033] The application will be specifically explained in combination with the specific embodiments.

[0034] The application inoculates and domesticates activated sludge of municipal sewage treatment plant, uses specific nutrient solution and flow state to select and enrich microorganisms, the formula of the nutrient solution is K2HPO4 1.0g·L -1 , NaHCO3 0.5g·L -1 , NH4Cl 0.05g·L -1 , Na2S·9H2O 0.225g·L -1 , trace elements 0.001g·L -1 , the composition of trace elements includes EDTA 0.5g·L -1 、

[0035] (NH4)6Mo7O 24 ·4H2O 0.01g·L -1 , ZnSO4·7H2O 0.04g·L -1 , MnCl2·7H2O 0.03g·L -1 , CuSO4·5H2O0.02g·L -1 , CoCl2·6H2O 0.02g·L -1 ; the synchronous desulfurization and deamination biological deodorization can be realized.

[0036] (1) activated sludge is obtained from the secondary treatment unit of the sewage treatment plant; (2) the inoculated sludge is domesticated, that is, the activated sludge in the aerobic tank of the A 2 / O treatment unit is pretreated by screening and elutriation, and then is placed in an upflow reactor for culture; (3) the activated sludge is continuously supplied with nutrient solution to provide nutrients for microorganisms, and the nutrient solution contains Na2S·9H2O and the like for selecting and enriching sulfur-oxidizing microorganisms; (4) when the sulfur-oxidizing activity of the inoculated microorganisms is stable, the domesticated sulfur-oxidizing microorganism mixed filler is used for biofilm formation, and then can be used for subsequent biological deodorization of the influent well of the municipal sewage treatment plant; (5) in the process of biological deodorization, the circulating liquid is acidic, and the abundance of sulfur-oxidizing bacteria is significantly higher than that of the traditional process. The application improves the deodorization performance of the municipal sewage treatment plant by using acidophilic sulfur-oxidizing bacteria for synchronous desulfurization and deamination, compared with the traditional deodorization process, the synchronous desulfurization and deamination process can enrich high-abundance sulfur-oxidizing bacteria in a short period of time, and has higher removal capacity for ammonia and hydrogen sulfide.

[0037] The sulfur-oxidizing bacteria enrichment method of the application comprises the following steps: the activated sludge from an aerobic tank is pretreated by screening and elutriation, and then is placed in an upflow reactor; and the activated sludge is continuously supplied with the sulfur-oxidizing bacteria screening and enrichment nutrient solution to screen and enrich the sulfur-oxidizing microorganisms. The method uses inoculated sludge and uses the upflow reactor and the nutrient solution to domesticate and enrich the sludge, so as to treat the odor of a sewage treatment plant. The domesticated and enriched sludge can improve the activity of the sulfur-oxidizing microorganisms, the circulating liquid is acidic in the biological deodorization process, so that the circulating liquid is more suitable for the growth environment of the subsequent sulfur-oxidizing bacteria. In addition, the method can be used for the packing biofilm formation and continuous test of a subsequent biological trickling filter tower, and provides the microorganism population that has adapted to specific environmental conditions for subsequent biological deodorization.

[0038] In the application, the height-diameter ratio of the reaction zone of the upflow reactor is 11, and the HRT is 12h.

[0039] In the application, the activated sludge from the aerobic tank is the activated sludge of a sewage treatment plant, and the indexes are as follows: ammonia nitrogen is 0.94mg·L -1 , total nitrogen is 0.28g·L -1 , total protein is 1.64g·L -1 , soluble protein is 19.23mg·L -1 , pH is 7.52, TS is 6.62g·L -1 , SS is 4.86g·L -1 , VSS is 1.78g·L -1 , TCOD is 5.77g·L -1 , and SCOD is 34.14mg·L -1 .

[0040] The biological deodorization method for simultaneous desulfurization and deamination of the application uses the microorganism mixed packing enriched by the sulfur-oxidizing bacteria enrichment method of the application to form a biofilm in a biological trickling filter tower.

[0041] In the application, the enriched microorganism is Thiomonas_arsenitoxydans, and the relative abundance is 40%.

[0042] In the application, the biological trickling filter tower adopts the gas-liquid countercurrent and series air inlet mode, the chemical washing tower is arranged at the rear part of the BTF in the simultaneous desulfurization and deamination mode, the chemical washing tower is arranged at the front part of the BTF in the chemical deamination mode, the circulating liquid enters from the top of the BTF and is discharged from the bottom of the BTF, and the circulating liquid volume update frequency is 50% per day.

[0043] In the aerobic condition, the circulating liquid used in the first 30 days is the enrichment nutrient solution, the circulating liquid is replaced with tap water in the 30th to 60th day, and the pH of the circulating liquid in the biological trickling filter tower is also acidic.

[0044] More specifically, the sign of biofilm completion is that the filler surface is attached with grayish white point-like substances. The circulating liquid update frequency is 50% per day. The circulating liquid temperature in the reactor is maintained at 30±1℃, and the sulfide in the circulating liquid is derived from Na2S·9H2O, and the circulating liquid is kept continuously watered.

[0045] The gases released by the municipal wastewater treatment plant are divided into four categories: the first category is hydrogen sulfide, mercaptans, sulfides and thiophenes, and other sulfur-containing compounds; the second category is ammonia, amines, amides and indoles, and other nitrogen-containing compounds; the third category is alkanes, alkenes, alkynes and aromatic hydrocarbons; and the fourth category is alcohols, aldehydes, ketones, phenols and oxygen-containing organic compounds. The average hourly concentrations of ammonia and hydrogen sulfide are 5.89-10.39 mg / m 3 and 1.45-2.96 mg / m 3 , respectively, and the maximum concentrations are 118.62 mg / m 3 and 24.89 mg / m 3 , respectively.

[0046] Example 1:

[0047] 1. Test device

[0048] The inoculated sludge enrichment part device comprises an upflow reactor. The height-diameter ratio of the reaction zone of the upflow reactor is 11, and the HRT is 12 h. The nutrient solution formula is K2HPO4 1.0 g·L -1 , NaHCO3 0.5 g·L -1 , NH4Cl 0.05 g·L -1 , Na2S·9H2O 0.225 g·L -1 , trace elements 0.001 g·L -1 , trace element composition includes EDTA 0.5 g·L -1 , (NH4)6Mo7O 24 ·4H2O 0.01 g·L -1 , ZnSO4·7H2O 0.04 g·L -1 , MnCl2·7H2O 0.03 g·L -1 , CuSO4·5H2O 0.02 g·L -1 , CoCl2·6H2O 0.02 g·L -1 . The circulating liquid temperature in the reactor is maintained at 30±1℃, and the sulfide in the circulating liquid is derived from Na2S·9H2O, and the circulating liquid is kept continuously watered.

[0049] The existing biological deodorization system uses external bacteria to start in the microbial enrichment stage. If the domestication method is used to start, it is often aimed at inoculated sludge containing rich microbial community, and the common nutrient solution formula is carbon source 0.5-1.0 g·L -1, nitrogen source 0.05-0.10 g·L -1 , phosphorus source 0.01-0.03 g·L -1 and a small amount of trace elements, wherein the ratio of carbon, nitrogen and phosphorus is usually controlled to be C:N:P=100:5:1.

[0050] The process study 1 nutrient solution formula of the application adopts K2HPO4 1.0 g·L -1 , NaHCO3 0.5 g·L -1 , NH4Cl 0.05 g·L -1 , Na2S·9H2O 0.225 g·L -1 , and the difference from the application is the lack of multiple trace elements. The bio-trickling filter system is started to treat odor using the formula, the dominant sulfur-oxidizing bacteria is Thiobacillus, the circulating liquid is alkaline (pH is about 8.5), and the desulfurization efficiency is 70.86±16.86%.

[0051] The nutrient solution formula in the process study 2 of the application is NaHCO3 2.0 g·L -1 , KH2PO4 0.036 g·L -1 , NH4Cl 0.016 g·L -1 , MgCl2·6H2O 0.028 g·L -1 , CaCl2·2H2O 0.018 g·L -1 and trace elements 2 mL·L -1 . The difference from the application is that, first, the constant element compounds are different, and the two differ in phosphorus source, nitrogen source, and whether they contain MgCl2·6H2O, CaCl2·2H2O and Na2S·9H2O, and second, the content of trace elements is different. The bio-trickling filter system is started to treat odor using the formula, the dominant sulfur-oxidizing bacteria is Halothiobacillus, Stenotrophomonas, Acidithiobacillus and Thiomonas, and the relative abundance is 59.3%, 11.62%, 8.49% and 3.99% in turn, the circulating liquid is weakly alkaline (pH 6.0-9.0), and the desulfurization efficiency is more than 90%.

[0052] The nutrient solution formula for comparative study is K2HPO4: 1.0 g·L -1 , KH2PO4: 1.0 g·L -1 , NaHCO3 0.4 g·L -1 , MgCl2·6H2O: 0.4 g·L -1 and C6H 12 O6: 4.0 g·L -1The difference between the present application and the prior art is that the constant elements and trace elements are different, and the trace elements in the comparative study are derived from pine bark filler. The biological trickling filter tower system is started to treat odor using the formula, the dominant sulfur-oxidizing bacteria is Thiobacillus, the circulating liquid is weakly alkaline (pH is maintained at 6.5-8.5), and the desulfurization efficiency is more than 85%.

[0053] 2. Test operation

[0054] A 2 The activated sludge in the aerobic tank of the / O treatment unit is pretreated by screening and elutriation, and then placed in an upflow reactor. Nutrient solution is continuously supplied to the activated sludge to provide nutrients for microorganisms. At the same time, Na2S·9H2O is added to select and enrich sulfur-oxidizing microorganisms. During the enrichment process, the removal of the inoculated sludge on the substrate needs to be determined, the basic operation of the reactor needs to be monitored, and the morphological changes of the sludge need to be observed. The activity of the inoculated sludge is determined regularly.

[0055] Table 1 Different nutrient solution ratios and differences in microbial structure

[0056]

[0057] Example 2:

[0058] 1. Test device

[0059] The deodorization continuous test device mainly includes two-stage BTF, circulating liquid cylinder, pH meter, hydrogen sulfide and ammonia gas detector, gas flow meter, in addition to circulating liquid circulating pump, air pump, etc. The deodorization main body includes BTF and acidic environment chemical washing tower.

[0060] 2. Test operation

[0061] In this test, the gas-liquid relative flow mode adopted by BTF is gas-liquid countercurrent, and the gas is introduced from the bottom of each filler zone and discharged at the top of the tower. The chemical washing tower is placed in the rear part of BTF in the synchronous desulfurization and deamination mode, and the chemical washing tower is placed in the front part of BTF in the chemical deamination mode. The circulating liquid enters from the top of BTF and is discharged from the bottom of BTF, with a replacement frequency of 50% per day.

[0062] In the first 30 days, sulfur-containing nutrient medium is used in aerobic conditions, and sulfur is converted into sulfate form, which makes the pH value of the circulating liquid decrease. In the 30th-60th day, the circulating liquid is replaced with tap water, and the pH of the circulating liquid in the biological trickling filter tower is also acidic.

[0063] Example 3:

[0064] The difference from Example 2 is that in the preliminary experiment, it is found that the pH value of the circulating liquid decreases obviously after the odor of the water inlet is treated by the method of simultaneous desulfurization and denitrification, and gradually stabilizes at 2-3, which deviates from the general cognition. The experiment is carried out by two simultaneous desulfurization and denitrification biological trickling towers to observe the change of the pH value of the circulating liquid. The laboratory enrichment of sulfur-oxidizing bacteria reactor is filled with membrane packing in X1 tower, and the water inlet gas (4L·m 3 ) is introduced into the X1 tower, and the pH value change of the circulating liquid in the X1 tower is measured.

[0065] The average pH value of the first to fifth day is 6.73, which is basically stable around 7.0, and the pH value decreases to 3.89 in the fifth to seventh day. This shows that the odor of the water inlet of the municipal sewage treatment plant is introduced into the biological trickling tower, and after about a week, the circulating liquid will be acidic.

[0066] The microorganisms on the packing in the water inlet odor-simultaneous desulfurization and denitrification biological trickling tower, after running for a week, the relative abundance of sulfur-oxidizing bacteria Thiomonas in the microbial community is 40%, and Alicyclobacillus acidophilus is the cause of the decrease of the pH value of the circulating liquid.

[0067] The above describes the preferred embodiments of the present disclosure in detail, but the present disclosure is not limited to the specific details in the above embodiments, and various simple modifications can be made to the technical solutions of the present disclosure within the technical concept of the present disclosure, and these simple modifications all belong to the protection scope of the present disclosure.

[0068] In addition, it should be noted that each specific technical feature described in the above specific embodiments can be combined in any appropriate manner without contradiction, and in order to avoid unnecessary repetition, the present disclosure will not further describe various possible combinations.

[0069] In addition, various different embodiments of the present disclosure can also be combined in any manner, as long as they do not deviate from the idea of the present disclosure, and they should also be considered as disclosed by the present disclosure.

Claims

1. A nutrient solution for screening and enriching sulfur-oxidizing bacteria, characterized in that, The nutrient enrichment solution was prepared at a ratio of K₂HPO₄ 1.0 g·L⁻¹. -1 NaHCO3 0.5g·L -1 NH4Cl 0.05 g·L -1 Na₂S·9H₂O 0.225 g·L⁻¹ -1 Trace elements 0.001 g·L -1 The trace element composition includes 0.5 g·L⁻¹ EDTA. -1 (NH4)6Mo7O 24 ·4H₂O 0.01g·L -1 ZnSO4·7H2O 0.04g·L -1 MnCl2·7H2O 0.03g·L -1 CuSO4·5H2O 0.02g·L -1 CoCl2·6H2O 0.02g·L -1 .

2. A method for enriching sulfur-oxidizing bacteria, characterized in that, include: After pretreatment by screening and washing, activated sludge from the aerobic tank is placed in an upflow reactor, and the sulfur-oxidizing microorganisms are continuously screened and enriched by the sulfur-oxidizing bacteria screening and enrichment nutrient solution described in claim 1.

3. The method for enriching sulfur-oxidizing bacteria according to claim 2, characterized in that, The upflow reactor has a reaction zone height-to-diameter ratio of 11 and an HRT of 12h.

4. The method for enriching sulfur-oxidizing bacteria according to claim 2 or 3, characterized in that, The activated sludge from the aerobic tank is activated sludge from a wastewater treatment plant, and its indicators include: ammonia nitrogen of 0.94 mg·L⁻¹. -1 The total nitrogen was 0.28 g·L. -1 Total protein content was 1.64 g / L. -1 The soluble protein content was 19.23 mg / L. -1 pH 7.52, TS 6.62 g·L -1 SS was 4.86 g·L -1 VSS is 1.78 g·L -1 TCOD was 5.77 g·L. -1 The SCOD was 34.14 mg·L⁻¹. -1 .

5. A biological deodorization method for simultaneous desulfurization and ammonia removal, characterized in that, Microbial mixed packing material enriched by the sulfur-oxidizing bacteria enrichment method according to any one of claims 1-4 is used for biofilm formation in a bio-trickling filter.

6. The biological deodorization method for simultaneous desulfurization and deammoniation according to claim 5, characterized in that, The microorganism mentioned is Thiomonas arsenitoxydans, with a relative abundance of 40%.

7. The biological deodorization method for simultaneous desulfurization and deammoniation according to claim 5, characterized in that, The bio-trickling filter tower adopts a gas-liquid countercurrent and series air intake method. The chemical scrubbing tower is placed after the BTF in the synchronous desulfurization and deammoniation method, while the chemical scrubbing tower is placed in front of the BTF in the traditional deodorization method. The circulating liquid enters from the top of the BTF and exits from the bottom of the BTF. The circulating liquid volume renewal frequency is 50% per day. Under aerobic conditions, the circulating liquid used from day 1 to day 30 is the sulfur-oxidizing bacteria screening and enrichment nutrient solution as described in claim 1; from day 30 to day 60, the circulating liquid is replaced with tap water, and the pH of the circulating liquid in the bio-trickling filter tower is acidic.

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