Application of structural variation marker of goat VRTN gene in goat breeding
By detecting the SNP sites of the VRTN gene in goats, and using PCR and sequencing technologies to screen for high-quality goats, the problem of lacking rapid screening of high-quality goats in existing technologies has been solved, enabling early identification of superior individuals and improving the efficiency of goat breeding.
Patent Information
- Application Number
- CN202511083245.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-04
- Publication Date
- 2025-11-11
AI Technical Summary
There is a lack of effective molecular markers in the current technology for the rapid screening and improvement of high-quality goats, especially for improving their growth and meat quality traits.
Using structural variation markers of the goat VRTN gene, specific primers were designed to perform PCR amplification and sequencing by detecting SNP sites at positions 17448006 and 17447632 of NC_030817.1, and goat individuals with excellent growth and meat quality traits were screened out.
It enables rapid and accurate screening of growth and meat quality traits in goats, allowing for the early identification of superior individuals, improving the efficiency of goat genetic resource selection, and shortening the selection cycle.
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Figure CN120924675A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of genetic agriculture breeding, and in particular to the application of a structural variation marker of the VRTN gene in goat breeding. Background Technology
[0002] Molecular markers are typically specific DNA sequences or variation sites in the genome. Their polymorphism among different individuals can be linked to phenotypic expression through genetic analysis. By analyzing the association between molecular markers and specific traits, it is possible to predict an individual's performance in that trait and to more accurately select individuals with ideal traits for breeding, thereby significantly shortening the breeding cycle and improving the efficiency of genetic improvement.
[0003] As third-generation molecular markers, single nucleotide polymorphisms (SNPs) mainly refer to polymorphisms caused by single nucleotide variations at the genomic level, including single base substitutions, deletions, or insertions, with single nucleotide substitutions being the most common (Zhao, 2012). Due to their large number, broad genomic coverage, representativeness, genetic stability, and ease of automated analysis, SNPs are widely used in identifying animal breeds and strains, studying phylogenetic relationships, and constructing genetic maps. Their application in conjunction with marker-assisted selection has greatly promoted molecular breeding in plants and animals (Liu, 2016). SNPs located in different genomic positions have significantly different functions. Non-synonymous coding SNPs affect protein structure or function by altering amino acid sequences in the coding region of a gene; the strength of their effect depends primarily on whether the variant site is located in a key functional or structural domain of the protein (Zheng, 2024). These variations interfere with the biological functions of key proteins and are closely related to various diseases and important economic traits (such as the reproductive, growth, and milk production performance of goats), thus serving as key molecular markers for population genetics research and molecular breeding.
[0004] VRTN is a protein-coding gene. GO analysis has shown that this gene has a specific DNA sequence binding site and transposase activity (Zhang Hui, 2018). In zebrafish, VRTN acts as a transcriptional repressor, independently regulating the expression of the bmp2b gene along the dorsoventral axis by binding to the regulatory sequence of the bmp2b gene, ensuring a normal concentration gradient of the bmp2b gene along the dorsoventral axis, thereby promoting normal embryonic dorsoventral axis development (SHAO M et al, 2017). Mikawa et al. in Japan used QTL fine mapping, microsatellite markers, haplotype linkage analysis, and linkage disequilibrium analysis to identify VRTN as a potential causal gene affecting the number of thoracic vertebrae (FAB Y et al, 2013) (MIKAWA S et al, 2011). Other studies have shown that the intramuscular fat content (IMF) of the longissimus dorsi muscle in pigs is significantly correlated with VRTN genotype. The average IMF of wild-type (WtWt) individuals (5.22%) was greater than that of WtQ (4.99%) and QQ (4.79%) individuals (Hirose K et al, 2013). Currently, there is limited research on the VRTN gene in goats.
[0005] With the increasing popularity of healthy eating concepts and the growing demand for high-quality protein, goat meat, with its low-fat and high-nutrient characteristics, is occupying an important position in the global meat consumption market. Therefore, the market needs a method to improve goat yield and quality. This invention, using two SNPs as genetic markers to select goats with superior growth and muscle development, can address this market need. Summary of the Invention
[0006] To address the shortcomings of existing technologies, the present invention aims to provide an application of a structural variation marker of the goat VRTN gene in goat breeding. The present invention discovers a structural variation marker of the goat VRTN gene that can provide a potential molecular marker for marker-assisted selection of goat meat quality traits, and can be used for goat genetic resource screening or improved breeding.
[0007] To achieve the above objectives, the present invention adopts the following technical solution: The application of a structural variation marker in the goat VRTN gene for goat breeding. The structural variation marker is located on the goat VRTN gene reference genome ASM170441v1, with NCBI sequence number NC_030817.1. The structural variation marker is located at bases 17448006 and 17447632 of NC_030817.1. The sequence of the structural variation marker with a mutation at base 17448006 is SEQ ID No: 01 GGCACTCTCAG, and the sequence of the structural variation marker with a mutation at base 17447632 is SEQ ID No: 02 GGGGGACCAAC. The structural variation marker is a single-base substitution marker. Individuals with the substitution of the structural variation marker in the goat genome exhibit meat quality traits inferior to wild-type individuals.
[0008] Furthermore, the aforementioned application of structural variation markers for the goat VRTN gene in goat breeding involves the following: the structural variation marker for goat VRTN is located at base 17448006 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a C>T base mutation; the structural variation marker is located at base chr1 at position 17447632 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a G>A base mutation.
[0009] Furthermore, the aforementioned structural variation marker of the goat VRTN gene is applied in goat breeding. The VRTN structural variant marker in goats with a C>T base mutation at position 17448006 of NC_030817.1 is significantly associated with growth traits such as chest width, chest circumference, and hip width, and is also significantly associated with carcass traits such as carcass weight and eye muscle area. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals. The VRTN structural variant marker in goats with a G>A base mutation at position 17447632 of NC_030817.1 is significantly associated with growth traits such as chest circumference and body weight, and is also significantly associated with carcass weight, water-holding capacity and shear force. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals.
[0010] Furthermore, the aforementioned structural variation marker of the goat VRTN gene is applied in goat breeding. In goats with a mutation at nucleotide 17448006 of NC_030817.1, wild-type individuals with genotype CC had superior chest width and body length compared to mutant individuals with genotypes CT and TT; mutant individuals with genotype TT had superior chest circumference and hip width compared to mutant individuals with genotype CT and wild-type individuals with genotype CC. In goats with a mutation at genotype 17447632 in NC_030817.1, wild-type individuals with genotype GG had a wider hip bone than mutant individuals with genotypes GA and AA; mutant individuals with genotype AA had a higher body weight than mutant individuals with genotype GA and wild-type individuals with genotype GG.
[0011] Furthermore, regarding the application of the aforementioned structural variation marker of the goat VRTN gene in goat breeding, the primers for detecting the structural variation marker of the goat VRTN gene are: Upstream primer: F1: 5'-GTCACTGTAGACACCGTGGG-3' SEQ ID No: 03; Downstream primer: R1: 5'-CTACTACAACTGGCGCCGAA-3' SEQ ID No: 04.
[0012] The aforementioned structural variation markers of the goat VRTN gene are applied to goat breeding, specifically in goat VRTN gene assisted breeding.
[0013] As a preferred embodiment, the aforementioned structural variation markers of the goat VRTN gene are applied to goat breeding, specifically in goat-assisted breeding kits.
[0014] A method for detecting goat meat quality traits includes the following steps: using the whole genome DNA of the goat to be tested as a template, using primer pair P1 as primers, amplifying partial fragments of the exon variant region of the goat VRTN gene by PCR, and then detecting the genotype by directly sequencing the PCR products. Primer pair P1 is: Upstream primer: F1: 5'-GTCACTGTAGACACCGTGGG-3' SEQ ID No: 03; Downstream primer: R1: 5'-CTACTACAACTGGCGCCGAA-3' SEQ ID No: 04.
[0015] The sequence of the structural variation marker is SEQ ID No:01. The structural variation marker is located on the goat VRTN gene reference genome ASM170441v1, with NCBI sequence number NC_030817.1. The position of the structural variation marker is at bases 17448006 and 17447632 of NC_030817.1. The structural variation marker is a single-base substitution marker. At the same age, the meat quality of goats with the substitution of the structural variation marker in their genome is inferior to that of wild-type individuals. The structural variation marker for goat VRTN is at base 17448006 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a C>T base mutation; the structural variation marker is at base chr1 at position 17447632 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a G>A base mutation.
[0016] Furthermore, the aforementioned method for detecting the quality traits of goat meat, The VRTN structural variant marker in goats with a C>T base mutation at position 17448006 of NC_030817.1 is significantly associated with growth traits such as chest width, chest circumference, and hip width, and is also significantly associated with carcass traits such as carcass weight and eye muscle area. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals. The VRTN structural variant marker in goats with a G>A base mutation at position 17447632 of NC_030817.1 is significantly associated with growth traits such as chest circumference and body weight, and is also significantly associated with carcass weight, water-holding capacity and shear force. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals.
[0017] Furthermore, the aforementioned method for detecting the quality traits of goat meat, In goats with a mutation at nucleotide 17448006 of NC_030817.1, wild-type individuals with genotype CC had superior chest width and body length compared to mutant individuals with genotypes CT and TT; mutant individuals with genotype TT had superior chest circumference and hip width compared to mutant individuals with genotype CT and wild-type individuals with genotype CC. In goats with a mutation at genotype 17447632 in NC_030817.1, wild-type individuals with genotype GG had a wider hip bone than mutant individuals with genotypes GA and AA; mutant individuals with genotype AA had a higher body weight than mutant individuals with genotype GA and wild-type individuals with genotype GG.
[0018] The advantages of this invention are: This invention has discovered SNP molecular markers at positions 17448006 and 17447632 of the VRTN gene in goats. Wild-type goats exhibit superior traits in chest width, chest girth, hip width, carcass weight, and eye muscle area compared to mutant individuals. This discovery can provide potential molecular markers for marker-assisted selection of goat meat quality traits and can be used to rapidly establish superior goat genetic resources, thereby accelerating the breed improvement process. This invention also found that in goats with a mutation at genotype 17448006 of NC_030817.1, wild-type individuals with the genotype CC had superior chest width and body length compared to mutant individuals with genotypes CT and TT; mutant individuals with genotype TT had superior chest circumference and hip width compared to mutant individuals with genotype CT and wild-type individuals with genotype CC; and wild-type goats with a mutation at genotype 17447632 of NC_030817.1, wild-type individuals with the genotype GG had superior hip width compared to mutant individuals with genotypes GA and AA; and mutant individuals with genotype AA had superior body weight compared to mutant individuals with genotype GA and wild-type individuals with genotype GG. These findings can also be applied to the screening and breeding of goats with specific individual traits such as hip size and body weight. The goat VRTN gene SNP detection method provided by this invention is not limited by age or sex, and can even be performed at birth, making it suitable for early goat breeding.
[0019] The primer set of this invention can accurately diagnose the SNP locus genotype of individual goats, and is fast, simple and low cost. Attached Figure Description
[0020] Figure 1 This is an electrophoresis image of the PCR amplification products of exon sequences 17448006 and 17447632 of the goat VRTN gene in an embodiment of the present invention.
[0021] Figure 2 This is a sequencing peak diagram of the CC, CT, and TT genotypes at position 17448006 on the exon sequence of the goat VRTN gene in an embodiment of the present invention.
[0022] Figure 3 This is a sequencing peak diagram of the GG, GA, and AA genotypes at position 17447632 on the exon sequence of the goat VRTN gene in an embodiment of the present invention. Detailed Implementation
[0023] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.
[0024] This invention utilizes the direct sequencing of PCR products to detect SNPs in the VRTN gene of goats and apply them to molecular breeding. The steps include: (1) using the VRTN gene sequence from the NCBI database, designing primers using the Primer-BLAST website, and verifying the primers using conventional PCR; (2) performing PCR amplification on the DNA sample, sending the obtained PCR products and primers to Qingke Biotechnology for sequencing, and comparing and analyzing the sequencing results using BioXM software; (3) using SPSS 25.0 software to perform association analysis between genotype and goat growth and meat quality traits, and screening out SNP markers related to goat growth and meat quality traits; (4) establishing a goat population with excellent growth and meat quality traits based on individual genotypes and conducting selective breeding.
[0025] The specific experimental steps and results are as follows: 1. Goat sample collection This invention uses Leizhou goats as the testing object, and collected and used ear tissue samples from 1200 female Hainan black goats (see Table 1). Eighty goats were randomly selected for slaughter, and their meat quality data, such as carcass weight, cross-sectional area of the longissimus dorsi muscle, water-holding capacity, and shear force, were measured and recorded for subsequent correlation analysis.
[0026] Table 1. Sample Information 2. Extraction of genomic DNA from ear tissue Genomic DNA was extracted using an animal tissue / cell genomic DNA extraction kit (Solarbio, Beijing, China). The steps are as follows: (1) Sample processing: Take 10mg of ear tissue, grind it into powder with liquid nitrogen, then suspend it fully in pre-cooled PBS or sterile water, then centrifuge at 12000rpm for 1min to collect cells, remove the supernatant as much as possible, add 200μL of solution A, and shake until thoroughly mixed.
[0027] (2) Add 20 μL of RNase A (10 mg / mL) to the suspension and place at 55 °C for 15 min.
[0028] (3) Add 20 μL of proteinase K (10 mg / mL), mix thoroughly by inverting, and digest overnight in a water bath at 55°C.
[0029] (4) Add 200 μL of solution B, invert and mix thoroughly. If a white precipitate appears, place it at 75℃ for 15-30 min and the precipitate will disappear.
[0030] (5) Add 200 μL of anhydrous ethanol and mix thoroughly. At this time, flocculent precipitate may appear, which will not affect DNA extraction. Both the solution and the flocculent precipitate can be added to the adsorption column.
[0031] (6) Centrifuge at 12000 rpm for 1 min, discard the waste liquid, and put the adsorption column into the collection tube.
[0032] (7) Add 600 μL of washing solution to the adsorption column (please check whether anhydrous ethanol has been added before use), and centrifuge at 12000 rpm for 1 min. Discard the waste liquid and place the adsorption column into the collection tube.
[0033] (8) Add 600 μL of washing solution to the adsorption column, centrifuge at 12000 rpm for 1 min, discard the waste liquid, and put the adsorption column into the collection tube.
[0034] (9) Centrifuge at 12000 rpm for 2 min, and place the adsorption column open at room temperature or in a 50℃ incubator for several minutes. The purpose is to remove the residual washing solution in the adsorption column. Otherwise, the ethanol in the washing solution will affect subsequent experiments such as enzyme digestion and PCR.
[0035] (10) Place the adsorption column into a clean centrifuge tube, add 50-200 μL of sterilized ultrapure water preheated in a 65°C water bath to the center of the adsorption membrane, let it stand at room temperature for 5 min, and centrifuge at 12000 rpm for 2 min.
[0036] (11) The sterilized ultrapure water obtained by centrifugation can be added to the adsorption column and centrifuged at 12000 rpm for 2 min to obtain high-quality genomic DNA.
[0037] 3. Design of target gene-specific primers Using the goat VRTN gene (NC_030817.1) published in the NCBI database (http: / / www.ncbi.nlm.nih.gov / ) as the reference sequence, the structural variant marker sequence for the mutation at position 17448006 is SEQ ID No: 01GGCACTCTCAG, amino acid mutation: p.490 R / K; the structural variant marker sequence for the mutation at position 17447632 is SEQ ID No: 02 GGGGGACCAAC, amino acid mutation: p.615. P / S, the structural variant markers were located on the goat VRTN gene reference genome ASM170441v1, NCBI sequence number: NC_030817.1. SNP variant sites identified during resequencing were located (structural variant markers at bases 17448006 and 17447632 of NC_030817.1). Specific primers were designed to amplify the fragment containing the polymorphic sites, with target fragment lengths of 951 bp (primer pair P1). Primer pair sequence information is shown in Table 2.
[0038] Table 2. Primer information for PCR 4. PCR amplification The PCR reaction system is shown in Table 3.
[0039] Table 3. PCR reaction system The PCR reaction procedure is as follows: (1) Pre-denature at 95 °C for 60 s, then proceed with the amplification reaction as described in (2); (2) Pre-denaturation at 94 °C for 60 s, denaturation at 95 °C for 30 s, annealing at 50 °C for 30 s, extension for 60 s, and then proceed with the amplification reaction as described in (3); (3) 95 °C denaturation for 30 s, 55 °C annealing for 30 s, extension for 60 s, cycle 45 times.
[0040] Primers suitable for PCR analysis were confirmed by agarose gel electrophoresis. Based on the electrophoresis results, the length of each sample band corresponded to the length of the target fragment. Figure 1 , Figure 2 ).
[0041] 5. Sequencing of PCR products and interpretation of results: The PCR products and primers were sequenced, and the sequencing results were analyzed using BioXM software. Genotype was determined based on the sequencing peak diagram. Figure 3As shown, when the peak diagram shows a single peak of G / A, the genotype is GG / AA; when the peak diagram shows a double peak of G and A, the genotype is GA.
[0042] The above results indicate that PCR amplification of goat genomic DNA using primers, followed by genotyping via direct sequencing, enables rapid and accurate identification of goat VRTN gene SNP sites.
[0043] 6. Association analysis of VRTN gene SNPs with growth and flesh quality traits Growth traits: body height, chest depth, chest width, body length, chest circumference, height at the cross, hip width, canal circumference, and body weight.
[0044] Meat characteristics: carcass weight, cross-sectional area of the longissimus dorsi muscle of the lumbar spine, water loss rate, water holding capacity, and shear rate.
[0045] Association analysis model: One-way ANOVA was performed using SPSS (25.0). The complete model is as follows: Ysj = μ + Gs + ej, Where Ysj is the phenotypic value, μ is the population mean, Gs is the genotype fixed effect, and ej is the random error.
[0046] The number of individuals of each type (Ref, Ref / Mut, and Mut) in the population was counted using one-way ANOVA with SPSS 25.0. The results are shown in Tables 4 and 5.
[0047] Table 4. Association analysis between VRTN gene SNP (chr1: 17448006) in Hainan Black Goat and growth and carcass traits. Association analysis results showed that the SNP variant site chr1: 17448006 of the goat VRTN gene was significantly associated with growth traits such as chest width, chest circumference and hip width, and was also significantly associated with carcass traits such as carcass weight and eye muscle area. Furthermore, wild-type individuals generally exhibited better phenotypic traits than mutant individuals.
[0048] Table 5. Association analysis between VRTN gene SNP (chr1:17447632) in Hainan Black Goat and growth and carcass traits. Association analysis results showed that the SNP site chr1:17447632 of the goat VRTN gene was significantly associated with growth traits such as chest circumference and body weight, and was also significantly associated with carcass weight, water-holding capacity and shear force. Furthermore, wild-type individuals generally exhibited better phenotypic traits than mutant individuals.
[0049] The above experiments show that this invention has discovered SNP molecular markers at positions 17448006 and 17447632 of the goat VRTN gene NC_030817.1. Since there are significant differences in growth traits among individuals with different genotypes, these SNPs can serve as candidate molecular genetic markers (SNPs) for effectively improving growth traits in goats. In actual production, this invention can be applied to the production of goat-assisted breeding kits. By eliminating individuals with inferior genotypes and retaining those with superior genotypes, the growth performance of the goat population can be gradually improved, further enabling the selection of larger-sized goats to increase yield, thereby achieving the effect of improving quality and efficiency.
[0050] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by equivalent substitution or equivalent transformation fall within the protection scope of the present invention.
Claims
1. The application of a structural variation marker for the VRTN gene in goat breeding, characterized in that, The structural variation markers are located on the goat VRTN gene reference genome ASM170441v1, with NCBI sequence number NC_030817.
1. The positions of the structural variation markers are bases 17448006 and 17447632 of NC_030817.
1. The sequence of the structural variation marker with a mutation at base 17448006 is SEQ ID No: 01 GGCACTCTCAG, and the sequence of the structural variation marker with a mutation at base 17447632 is SEQ ID No: 02 GGGGGACCAAC. The structural variation markers are single-base substitution markers. The meat quality of individuals with the substitution of the structural variation markers in the goat genome is inferior to that of wild-type individuals.
2. The application of a structural variation marker for the goat VRTN gene according to claim 1 in goat breeding, characterized in that, The structural variation marker for the goat VRTN is located at base 17448006 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a C>T base mutation; the structural variation marker is located at base chr1 at position 17447632 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a G>A base mutation.
3. The application of a structural variation marker for the goat VRTN gene according to claim 1 in goat breeding, characterized in that, The VRTN structural variant marker in goats with a C>T base mutation at position 17448006 of NC_030817.1 is significantly associated with growth traits such as chest width, chest circumference, and hip width, and is also significantly associated with carcass traits such as carcass weight and eye muscle area. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals. The VRTN structural variant marker in goats with a G>A base mutation at position 17447632 of NC_030817.1 is significantly associated with growth traits such as chest circumference and body weight, and is also significantly associated with carcass weight, water-holding capacity and shear force. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals.
4. The application of a structural variation marker for the goat VRTN gene according to claim 1 in goat breeding, characterized in that, In goats with a mutation at nucleotide 17448006 of NC_030817.1, wild-type individuals with genotype CC had superior chest width and body length compared to mutant individuals with genotypes CT and TT; mutant individuals with genotype TT had superior chest circumference and hip width compared to mutant individuals with genotypes CT and wild-type individuals with genotype TT. In goats with a mutation at genotype 17447632 in NC_030817.1, wild-type individuals with genotype GG had a wider hip bone than mutant individuals with genotypes GA and AA; mutant individuals with genotype AA had a higher body weight than mutant individuals with genotype GA and wild-type individuals with genotype GG.
5. The application of a structural variation marker for the goat VRTN gene according to claim 1 in goat breeding, characterized in that, The primers used to detect the structural variation markers of the goat VRTN gene are: Upstream primer: F1: 5'-GTCACTGTAGACACCGTGGG-3' SEQ ID No: 03; Downstream primer: R1: 5'-CTACTACAACTGGCGCCGAA-3' SEQ ID No:
04.
6. The application of the structural variation marker of the goat VRTN gene according to any one of claims 1-5 in goat breeding, characterized in that, The structural variation markers of the goat VRTN gene are used in goat-assisted breeding.
7. The application of the structural variation marker of the goat VRTN gene according to claim 6 in goat breeding, characterized in that, The structural variation markers of the goat VRTN gene are used in a goat-assisted breeding kit.
8. A method for detecting the quality traits of goat meat, characterized in that, The steps include: using the whole genome DNA of the goat to be tested as a template, using primer pair P1 as primers, amplifying partial fragments of the exon variant region of the goat VRTN gene by PCR, and then detecting the genotype by directly sequencing the PCR products; The primer pair P1 is: Upstream primer: F1: 5'-GTCACTGTAGACACCGTGGG-3' SEQ ID No: 03; Downstream primer: R1: 5'-CTACTACAACTGGCGCCGAA-3' SEQ ID No: 04; The sequence of the structural variation marker is SEQ ID No:
01. The structural variation marker is located on the goat VRTN gene reference genome ASM170441v1, with NCBI sequence number NC_030817.
1. The position of the structural variation marker is at bases 17448006 and 17447632 of NC_030817.
1. The structural variation marker is a single-base substitution marker. At the same age, the meat quality of individuals with the substitution of the structural variation marker in the goat genome is inferior to that of wild-type individuals. The structural variation marker for the goat VRTN is located at base 17448006 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a C>T base mutation; the structural variation marker is located at base chr1 at position 17447632 of NC_030817.1, and the structural variation marker is a single-base substitution marker, with the substitution marker being a G>A base mutation.
9. The method for detecting the quality traits of goat meat according to claim 8, characterized in that, The VRTN structural variant marker in goats with a C>T base mutation at position 17448006 of NC_030817.1 is significantly associated with growth traits such as chest width, chest circumference, and hip width, and is also significantly associated with carcass traits such as carcass weight and eye muscle area. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals. The VRTN structural variant marker in goats with a G>A base mutation at position 17447632 of NC_030817.1 is significantly associated with growth traits such as chest circumference and body weight, and is also significantly associated with carcass weight, water-holding capacity and shear force. Furthermore, wild-type individuals generally exhibit better phenotypic traits than mutant individuals.
10. A method for detecting the quality traits of goat meat according to claim 8, characterized in that, In goats with a mutation at nucleotide 17448006 of NC_030817.1, wild-type individuals with genotype CC had superior chest width and body length compared to mutant individuals with genotypes CT and TT; mutant individuals with genotype TT had superior chest circumference and hip width compared to mutant individuals with genotype CT and wild-type individuals with genotype CC. In goats with a mutation at genotype 17447632 in NC_030817.1, wild-type individuals with genotype GG had a wider hip bone than mutant individuals with genotypes GA and AA; mutant individuals with genotype AA had a higher body weight than mutant individuals with genotype GA and wild-type individuals with genotype GG.