Composition with anti-wrinkle and firming effects and application thereof

Abstract

The invention relates to a composition with anti-wrinkle and firming effects and application thereof. The composition is prepared from a brassica juncea leaf extract, a swertia indica extract, a kangaroo claw extract, a tomato fruit extract and polydeoxyribonucleotide. According to the invention, it is creatively found that the Brachirita leaf extract, the swertia indica extract, the kangaroo claw extract, the tomato fruit extract and the polydeoxyribonucleotide have a synergistic effect in the aspects of improving wrinkle resistance and tightening of skin. The composition can significantly reduce the ratio of crow's feet and nasolabial folds, improves the skin elasticity, and has excellent anti-wrinkle and firming effects.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition with anti-wrinkle and firming effects and its application. Background Technology

[0002] Skin aging is a complex process caused by both endogenous aging and exogenous photoaging. With age or external stimuli, the synthesis rate of type I and type III collagen in the dermis decreases, while the activity of matrix metalloproteinases abnormally increases, leading to the gradual collapse of the skin's supporting structure and a loss of elasticity and firmness. Among environmental factors, ultraviolet radiation is a significant contributor to collagen loss. Photoaging is a major cause of skin aging. Prolonged exposure to ultraviolet light and other forms of radiation can cause wrinkles, sagging, and premature aging. Most anti-wrinkle products only work by promoting collagen activity to achieve their anti-wrinkle effect.

[0003] CN108158867A discloses an anti-aging cosmetic and its preparation method. The cosmetic contains vitamin E, yeast extract and hydrolyzed protein complex, purified water, xanthan gum, sodium hyaluronate, 1,3-butanediol, sorbitol, panthenol, trehalose and vitamin B3, emollients, emulsifiers, hexanediol, cibarrel, methyl gluceth-20, vitamin A, vitamin C ethyl ether, and preservatives. This anti-aging cosmetic has been verified to have good wrinkle-reducing and anti-aging effects. However, the efficacy of this anti-aging cosmetic is complex and it carries potential safety risks.

[0004] CN113662891A discloses the application of a potent anti-aging loquat flower extract in the preparation of anti-aging cosmetics. The preparation method of the loquat flower extract is as follows: loquat flowers are pulverized, an extraction solvent is added, and extraction is performed using a room temperature high-pressure homogenization method to obtain an extract. After centrifugation, the centrifuged liquid is adsorbed onto a macroporous resin, eluted with an ethanol-water solution, and the eluent is concentrated under reduced pressure and then 1,3-butanediol is added to obtain the loquat flower extract. The loquat flower extract obtained by this method is natural and safe, and can effectively promote the proliferation and growth of human embryonic lung fibroblasts, effectively scavenge free radicals, inhibit tyrosinase activity, reduce melanin production, accelerate the renewal of skin surface cells, and delay skin aging. When added to anti-aging cosmetics, it produces stable and effective anti-aging cosmetics with significant anti-aging effects.

[0005] However, existing anti-aging cosmetics often contain relatively simple active ingredients and lack a combination of multi-dimensional effects. Therefore, developing a highly effective composition with anti-aging and anti-wrinkle properties has become one of the urgent technical problems to be solved. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a composition with anti-wrinkle and firming effects and its application. This composition can significantly reduce the proportion of crow's feet and nasolabial folds, improve skin elasticity, and has excellent anti-wrinkle and firming effects.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a composition having anti-wrinkle and firming effects, the composition comprising Balkan gesneriaceae leaf extract, Indian swert extract, kangaroo paw extract, tomato fruit extract, and polydeoxyribonucleotides.

[0009] Balkan gesneriaceae leaf extract is rich in antioxidant polyphenols and flavonoids, which can neutralize free radicals, delay photoaging, and enhance the skin barrier; Indian swert extract contains swertinoin and mangiferin, which have anti-inflammatory effects and can regulate the skin's microecological balance; kangaroo paw extract contains natural polysaccharides and anthocyanins, which have strong moisturizing and water-locking abilities and can brighten the complexion; tomato fruit extract is rich in high concentrations of lycopene, which has antioxidant effects, reduces UV damage, and promotes collagen synthesis; polydeoxyribonucleotides derived from fish essence or plant DNA can promote cell repair and metabolism and delay aging.

[0010] This invention creatively discovers that Balkan gesneriad leaf extract, Indian swert sprout extract, kangaroo paw extract, tomato fruit extract, and polydeoxyribonucleotides have significant synergistic effects in anti-wrinkle and firming.

[0011] Preferably, the composition comprises, by weight parts, 1-5 parts of Balkan gesneriad leaf extract, 1-5 parts of Indian swert sprout extract, 1-5 parts of kangaroo paw extract, 1-5 parts of tomato fruit extract, and 0.2-1 parts of polydeoxyribonucleotides.

[0012] Among them, the specific point values ​​for 1-5 parts can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc., and the specific point values ​​for 0.2-1 parts can be 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, etc.

[0013] Preferably, the Balkan gesneriaceae leaf extract is prepared using a method comprising the following steps:

[0014] (1) Balkan gesneriaceae leaf raw material was mixed with water and subjected to ultra-high pressure micro-jet pretreatment to obtain pretreated solution;

[0015] (2) The pretreatment solution was mixed with the compound enzyme to carry out the enzymatic hydrolysis reaction to obtain the enzymatic hydrolysate. The enzymatic hydrolysate was filtered and the filtrate was collected to obtain Balkan gesneriaceae leaf extract.

[0016] This invention utilizes ultra-high pressure microjet pretreatment of Balkan gesneriad leaves to better extract the active ingredients from the plant material, thereby achieving excellent anti-wrinkle and firming effects.

[0017] Preferably, the ratio of Balkan gesneriaceae leaf raw material to water in step (1) is 1:(20-40)g / mL, for example, it can be 1:20g / mL, 1:25g / mL, 1:30g / mL, 1:35g / mL, 1:40g / mL, etc.

[0018] Preferably, the temperature of the ultra-high pressure microjet pretreatment in step (1) is 50-70℃, the pressure of the ultra-high pressure microjet pretreatment is 100-400MPa, and the number of ultra-high pressure microjet pretreatments is 1-4 times.

[0019] Among them, the specific point values ​​in the 50-70℃ range can be 50℃, 55℃, 60℃, 65℃, 70℃, etc.; the specific point values ​​in the 100-400MPa range can be 100MPa, 200MPa, 300MPa, 400MPa, etc.; and the specific point values ​​in the 1-4 cycles can be 1, 2, 3, or 4 cycles.

[0020] Preferably, the complex enzyme in step (2) includes hemicellulase, β-glucosidase and tanninase.

[0021] Hemicellulase can break down hemicellulose such as xylan and mannitol, disrupt plant cell wall structure, and promote the release of active ingredients; β-glucosidase can hydrolyze glycosidic bonds to release bound active ingredients; tanninase can hydrolyze tannic acid and its derivatives, reducing the tannin content of plant extracts.

[0022] This invention creatively discovers that hemicellulase, β-glucosidase, and tanninase have a certain synergistic effect on the enzymatic hydrolysis of Balkan gesneriad leaves. Enzymatic hydrolysis of the pretreated solution of Balkan gesneriad leaves with this complex enzyme can further improve the anti-wrinkle effect of the prepared Balkan gesneriad leaf extract.

[0023] Preferably, the mass ratio of hemicellulase, β-glucosidase and tanninase is (1-5):(5-10):(0.1-0.5).

[0024] Among them, the specific point values ​​in 1-5 can be 1, 2, 3, 4, 5, etc., the specific point values ​​in 5-10 can be 5, 6, 7, 8, 9, 10, etc., and the specific point values ​​in 0.1-0.5 can be 0.1, 0.2, 0.3, 0.4, 0.5, etc.

[0025] Preferably, the mass ratio of the pretreatment solution to the complex enzyme in step (2) is 100:(1-5), for example, it can be 100:1, 100:2, 100:3, 100:4, 100:5, etc.

[0026] Preferably, the temperature of the enzymatic hydrolysis reaction in step (2) is 40-60℃, and the time of the enzymatic hydrolysis reaction is 0.5-2h.

[0027] Among them, the specific point values ​​in the 40-60℃ range can be 40℃, 45℃, 50℃, 55℃, 60℃, etc., and the specific point values ​​in the 0.5-2h range can be 0.5h, 1h, 1.5h, 2h, etc.

[0028] Preferably, the kangaroo paw extract is prepared using a method comprising the following steps:

[0029] (I) Kangaroo paw raw material was mixed with water and microwaved for extraction. The filtrate was collected by filtration to obtain crude kangaroo paw extract.

[0030] (II) The crude extract of kangaroo paw was mixed with a flocculant for flocculation treatment, and the supernatant was collected by centrifugation to obtain the kangaroo paw extract.

[0031] This invention utilizes microwave extraction of kangaroo paw raw materials to better extract the active ingredients from the plant materials, thereby achieving excellent anti-wrinkle and firming effects.

[0032] Preferably, the ratio of kangaroo paw raw material to water in step (I) is 1:(30-60)g / mL, for example, it can be 1:30g / mL, 1:35g / mL, 1:40g / mL, 1:45g / mL, 1:50g / mL, 1:55g / mL, 1:60g / mL, etc.

[0033] Preferably, the microwave extraction temperature in step (I) is 50-80℃, the microwave extraction power is 200-500W, and the microwave extraction time is 10-30min.

[0034] Specific values ​​for the 50-80℃ range can be selected from 50℃, 55℃, 60℃, 65℃, 70℃, 75℃, 80℃, etc.; specific values ​​for the 200-500W range can be selected from 200W, 250W, 300W, 350W, 400W, 450W, 500W, etc.; and specific values ​​for the 10-30min range can be selected from 10min, 15min, 20min, 25min, 30min, etc.

[0035] Preferably, the flocculant in step (II) includes chitosan, sodium carboxymethyl starch, and guar gum.

[0036] In the preparation of kangaroo paw extract, chitosan, sodium carboxymethyl starch and guar gum are used as a compound flocculant to flocculate the crude kangaroo paw extract, which can further enhance the enrichment effect of active ingredients, improve the anti-wrinkle effect of the extract, and improve the stability of the product.

[0037] Preferably, the mass ratio of chitosan, sodium carboxymethyl starch and guar gum is 1:(0.5-1):(1-3).

[0038] Among them, the specific point values ​​in 0.5-1 can be 0.5, 0.6, 0.7, 0.8, 0.9, 1, etc., and the specific point values ​​in 1-3 can be 1, 1.5, 2, 2.5, 3, etc.

[0039] Preferably, the mass ratio of the kangaroo paw crude extract to the flocculant in step (II) is 100:(0.1-2), for example, it can be 100:0.1, 100:0.5, 100:1, 100:1.5, 100:2, etc.

[0040] Preferably, the Indian swert sprout extract is prepared using a method comprising the following steps:

[0041] The raw material of Indian swert was mixed with water and subjected to hot extraction. The supernatant was collected by centrifugation to obtain Indian swert extract.

[0042] Preferably, the ratio of the Indian swert sprout raw material to water is 1:(20-50)g / mL, for example, it can be 1:20g / mL, 1:25g / mL, 1:30g / mL, 1:35g / mL, 1:40g / mL, 1:45g / mL, 1:50g / mL, etc.

[0043] Preferably, the hot extraction temperature is 60-90℃ and the hot extraction time is 0.5-2.5h.

[0044] Among them, the specific point values ​​in the 60-90℃ range can be 60℃, 70℃, 80℃, 90℃, etc., and the specific point values ​​in the 0.5-2.5h range can be 0.5h, 1h, 1.5h, 2h, 2.5h, etc.

[0045] Preferably, the tomato fruit extract is prepared using a method comprising the following steps:

[0046] The tomato fruit raw material was mixed with an aqueous solution of ethanol and subjected to hot reflux extraction. The filtrate was collected by filtration to obtain tomato fruit extract.

[0047] Preferably, the ratio of the tomato fruit raw material to the aqueous ethanol solution is 1:(10-30)g / mL, for example, it can be 1:10g / mL, 1:15g / mL, 1:20g / mL, 1:25g / mL, 1:30g / mL, etc.

[0048] Preferably, the hot reflux extraction is performed 1-3 times, and the hot reflux extraction time for each extraction is 1.5-3 hours.

[0049] Among them, the specific point values ​​for 1-3 times can be 1 time, 2 times or 3 times, and the specific point values ​​for 1.5-3h can be 1.5h, 2h, 2.5h, 3h, etc.

[0050] Preferably, the volume percentage of ethanol in the aqueous solution of ethanol is 60-80%, for example, it can be 60%, 65%, 70%, 75%, 80%, etc.

[0051] In a second aspect, the present invention provides the application of the composition having anti-wrinkle and firming effects as described in the first aspect in cosmetics.

[0052] The numerical range described in this invention includes not only the point values ​​listed above, but also any point values ​​within the numerical ranges not listed above. Due to space limitations and for the sake of brevity, this invention will not exhaustively list all the specific point values ​​included in the range.

[0053] Compared with the prior art, the present invention has the following beneficial effects:

[0054] This invention creatively develops a composition with anti-wrinkle and firming effects, comprising Balkan gesneriad leaf extract, Indian swert sprout extract, kangaroo paw extract, tomato fruit extract, and polydeoxyribonucleotides; and experiments have demonstrated that the above components have significant synergistic effects in anti-wrinkle and firming.

[0055] Furthermore, in the preparation of Balkan gesneriad leaf extract, by subjecting the Balkan gesneriad leaves to ultra-high pressure microfluidic pretreatment, the effective components in the plant raw materials can be extracted more effectively, thereby exerting excellent anti-wrinkle and firming effects.

[0056] Furthermore, during the preparation of Balkan gesneriad leaf extract, hemicellulase, β-glucosidase, and tanninase exhibit a certain synergistic effect in the enzymatic hydrolysis of Balkan gesneriad leaves. Enzymatic hydrolysis of the pretreated solution of Balkan gesneriad leaves with this complex enzyme can further improve the anti-wrinkle effect of the prepared Balkan gesneriad leaf extract.

[0057] Furthermore, in the preparation of kangaroo paw extract, microwave extraction of kangaroo paw raw materials can better extract the active ingredients from the plant materials, thereby exerting excellent anti-wrinkle and firming effects.

[0058] Meanwhile, in the preparation of kangaroo paw extract, chitosan, sodium carboxymethyl starch and guar gum are used as a compound flocculant to flocculate the crude kangaroo paw extract, which can further enhance the enrichment effect of active ingredients, improve the anti-wrinkle effect of the extract, and improve the stability of the product. Detailed Implementation

[0059] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0060] The sources of each component / raw material in the following embodiments are shown in Table 1.

[0061] Table 1

[0062]

[0063] Preparation Example 1-1

[0064] This preparation example provides a Balkan primrose leaf extract, which is prepared using the following method:

[0065] (1) Wash the leaves of Balkan gesneriaceae with clean water, dry them and crush them. Mix them with water at a material-to-liquid ratio of 1:30 g / mL and perform ultra-high pressure micro-jet pretreatment (temperature 60℃, pressure 200MPa, number of times 2) to obtain the pretreated solution.

[0066] (2) The pretreatment solution was mixed with a compound enzyme (hemicellulase, β-glucosidase and tanninase in a mass ratio of 3:7:0.3) for enzymatic hydrolysis. The amount of compound enzyme added was 3% of the mass of the pretreatment solution. The temperature of the enzymatic hydrolysis reaction was 50℃ and the reaction time was 1h. After the reaction, the temperature was raised to 90℃ and the enzyme was deactivated for 30min to obtain the enzymatic hydrolysate.

[0067] (3) The enzymatic hydrolysate was filtered through a ceramic membrane with a pore size of 160 nm, the filtrate was concentrated under reduced pressure to 20 wt%, sterilized, and freeze-dried to obtain Balkan gesneriaceae leaf extract.

[0068] Preparation Examples 1-2

[0069] This preparation example provides a Balkan primrose leaf extract, which is prepared using the following method:

[0070] (1) Wash the leaves of Balkan gesneriaceae with clean water, dry them and crush them. Mix them with water at a material-to-liquid ratio of 1:20 g / mL and perform ultra-high pressure micro-jet pretreatment (temperature 50℃, pressure 100MPa, number of times 4 times) to obtain the pretreated solution.

[0071] (2) The pretreatment solution was mixed with a compound enzyme (hemicellulase, β-glucosidase and tanninase in a mass ratio of 5:5:0.1) for enzymatic hydrolysis. The amount of compound enzyme added was 1% of the mass of the pretreatment solution. The temperature of the enzymatic hydrolysis reaction was 40℃ and the reaction time was 0.5h. After the reaction, the temperature was raised to 90℃ and the enzyme was deactivated for 30min to obtain the enzymatic hydrolysate.

[0072] (3) The enzymatic hydrolysate was filtered through a ceramic membrane with a pore size of 160 nm, the filtrate was concentrated under reduced pressure to 20 wt%, sterilized, and freeze-dried to obtain Balkan gesneriaceae leaf extract.

[0073] Preparation Examples 1-3

[0074] This preparation example provides a Balkan primrose leaf extract, which is prepared using the following method:

[0075] (1) Wash the leaves of Balkan gesneriaceae with clean water, dry them and crush them. Mix them with water at a material-to-liquid ratio of 1:40 g / mL and perform ultra-high pressure micro-jet pretreatment (temperature 70℃, pressure 400 MPa, number of times 1) to obtain the pretreated solution.

[0076] (2) The pretreatment solution was mixed with a compound enzyme (hemicellulase, β-glucosidase and tanninase in a mass ratio of 1:10:0.5) for enzymatic hydrolysis. The amount of compound enzyme added was 5% of the mass of the pretreatment solution. The temperature of the enzymatic hydrolysis reaction was 60℃ and the reaction time was 2h. After the reaction, the temperature was raised to 90℃ and the enzyme was deactivated for 30min to obtain the enzymatic hydrolysate.

[0077] (3) The enzymatic hydrolysate was filtered through a ceramic membrane with a pore size of 160 nm, the filtrate was concentrated under reduced pressure to 20 wt%, sterilized, and freeze-dried to obtain Balkan gesneriaceae leaf extract.

[0078] Preparation Examples 1-4

[0079] This preparation example provides a Balkan gesneriad leaf extract, the only difference from Preparation Example 1-1 being that the Balkan gesneriad leaves are mixed with water and then subjected to ultrasonic pretreatment (ultrasonic extraction temperature of 60℃, power of 400W, and time of 60min) to obtain a pretreated solution. The remaining steps are consistent with Preparation Example 1-1.

[0080] Preparation Examples 1-5

[0081] This preparation example provides a Balkan gesneriaceae leaf extract, the only difference of which is that the total amount of the compound enzyme is kept the same, and the compound enzyme is adjusted to a mass ratio of hemicellulase and β-glucosidase of 3:7. The remaining steps are the same as those in preparation example 1-1.

[0082] Preparation Examples 1-6

[0083] This preparation example provides a Balkan gesneriaceae leaf extract, the only difference of which is that the total amount of the compound enzyme is kept constant, and the compound enzyme is adjusted to a mass ratio of hemicellulase and tanninase of 3:0.3. The remaining steps are the same as those in preparation example 1-1.

[0084] Preparation Examples 1-7

[0085] This preparation example provides a Balkan gesneriaceae leaf extract, the only difference of which is that the total amount of the complex enzyme is kept constant, and the complex enzyme is adjusted to be β-glucosidase and tanninase in a mass ratio of 7:0.3. The remaining steps are the same as those in preparation example 1-1.

[0086] Preparation Example 2-1

[0087] This preparation example provides a kangaroo paw extract, which is prepared using the following method:

[0088] (1) Crush the kangaroo paw and mix it with water at a ratio of 1:45 g / mL. Microwave extraction was performed (the temperature of microwave extraction was 60℃, the power was 400W, and the time was 20min). The extract was filtered with gauze and the filtrate was collected to obtain crude kangaroo paw extract.

[0089] (2) Take 1% of the mass of the crude kangaroo paw extract flocculant (composed of 1 part chitosan, 0.8 parts sodium carboxymethyl starch and 2 parts guar gum by weight), heat and dissolve the flocculant with water, cool it and mix it with the crude kangaroo paw extract. Stir at 30°C for 1 h for flocculation treatment, centrifuge at 2000 rpm for 30 min, collect the supernatant, concentrate the supernatant under reduced pressure to 20 wt%, sterilize and freeze dry to obtain the kangaroo paw extract.

[0090] Preparation Example 2-2

[0091] This preparation example provides a kangaroo paw extract, which is prepared using the following method:

[0092] (1) Crush the kangaroo paw and mix it with water at a ratio of 1:30 g / mL. Microwave extraction was performed (the temperature of microwave extraction was 50℃, the power was 200W, and the time was 30min). The extract was filtered with gauze and the filtrate was collected to obtain crude kangaroo paw extract.

[0093] (2) Take 0.1% of the mass of the crude kangaroo paw extract flocculant (composed of 1 part chitosan, 0.5 parts sodium carboxymethyl starch and 3 parts guar gum by weight), heat and dissolve the flocculant with water, cool it and mix it with the crude kangaroo paw extract. Stir at 20°C for 2 hours for flocculation treatment, centrifuge at 1000 rpm for 40 minutes, collect the supernatant, concentrate the supernatant under reduced pressure to 20 wt%, sterilize and freeze dry to obtain the kangaroo paw extract.

[0094] Preparation Examples 2-3

[0095] This preparation example provides a kangaroo paw extract, which is prepared using the following method:

[0096] (1) Crush the kangaroo paw and mix it with water at a ratio of 1:60 g / mL. Microwave extraction was performed (the temperature of microwave extraction was 80℃, the power was 500W, and the time was 10min). The extract was filtered with gauze and the filtrate was collected to obtain crude kangaroo paw extract.

[0097] (2) Take 2% of the mass of the crude kangaroo paw extract flocculant (composed of 1 part chitosan, 1 part sodium carboxymethyl starch and 1 part guar gum by weight), heat and dissolve the flocculant with water, cool it and mix it with the crude kangaroo paw extract, stir at 40°C for 0.5 h for flocculation treatment, centrifuge at 3000 rpm for 20 min, collect the supernatant, concentrate the supernatant under reduced pressure to 20 wt%, sterilize, freeze dry to obtain kangaroo paw extract.

[0098] Preparation Examples 2-4

[0099] This preparation example provides a kangaroo paw extract. The only difference between this preparation method and that of Preparation Example 2-1 is that after the kangaroo paw is mixed with water, ultrasonic extraction is performed (the ultrasonic extraction temperature is 60°C, the power is 400W, and the time is 20min) to obtain the extract. The remaining steps are the same as those in Preparation Example 2-1.

[0100] Preparation Examples 2-5

[0101] This preparation example provides a kangaroo paw extract, the only difference between which is the preparation method and that of Preparation Example 2-1. The amount of flocculant added is kept constant, and the flocculant is adjusted to consist of 1 part chitosan and 0.8 parts sodium carboxymethyl starch by weight. The remaining steps are consistent with those of Preparation Example 2-1.

[0102] Preparation Examples 2-6

[0103] This preparation example provides a kangaroo paw extract, the only difference between which is the preparation method and that of Preparation Example 2-1. The amount of flocculant added is kept constant, and the flocculant is adjusted to consist of 1 part chitosan and 2 parts guar gum by weight. The remaining steps are consistent with those of Preparation Example 2-1.

[0104] Preparation Examples 2-7

[0105] This preparation example provides a kangaroo paw extract, the only difference from preparation example 2-1 is that the amount of flocculant added is kept the same, and the flocculant is adjusted to consist of 0.8 parts by weight of sodium carboxymethyl starch and 2 parts by weight of guar gum. The remaining steps are the same as in preparation example 2-1.

[0106] Preparation Example 3-1

[0107] This preparation example provides an extract of *Swertia macrantha* sprouts, prepared using the following method:

[0108] Indian swert sprouts were pulverized and mixed with water at a ratio of 1:35 g / mL. The mixture was then subjected to hot extraction (80°C for 1.5 h). The extract was centrifuged at 8000 rpm for 10 min, and the supernatant was collected. The supernatant was concentrated under reduced pressure to 20 wt%, sterilized, and freeze-dried to obtain Indian swert sprout extract.

[0109] Preparation Example 3-2

[0110] This preparation example provides an extract of *Swertia macrantha* sprouts, prepared using the following method:

[0111] Indian swert sprouts were pulverized and mixed with water at a material-to-liquid ratio of 1:20 g / mL. The mixture was then subjected to hot extraction (60°C for 2.5 h). The extract was centrifuged at 5000 rpm for 10 min, and the supernatant was collected. The supernatant was concentrated under reduced pressure to 20 wt%, sterilized, and freeze-dried to obtain Indian swert sprout extract.

[0112] Preparation Example 3-3

[0113] This preparation example provides an extract of *Swertia macrantha* sprouts, prepared using the following method:

[0114] Indian swert sprouts were pulverized and mixed with water at a ratio of 1:50 g / mL. The mixture was then subjected to hot extraction (90°C for 0.5 h). The extract was centrifuged at 10,000 rpm for 5 min, and the supernatant was collected. The supernatant was concentrated under reduced pressure to 20 wt%, sterilized, and freeze-dried to obtain Indian swert sprout extract.

[0115] Preparation Example 4-1

[0116] This preparation example provides a tomato fruit extract, which is prepared using the following method:

[0117] Wash and crush the tomatoes to make a tomato paste. Mix the paste with a 70% ethanol aqueous solution at a material-to-liquid ratio of 1:20 g / mL and perform hot reflux extraction twice, each extraction lasting 2 hours. Combine the extracts, filter, concentrate under reduced pressure to 20 wt%, sterilize, and freeze-dry to obtain the tomato fruit extract.

[0118] Preparation Example 4-2

[0119] This preparation example provides a tomato fruit extract, which is prepared using the following method:

[0120] Wash and crush the tomatoes to make a tomato paste. Mix the paste with a 60% (v / v) ethanol aqueous solution at a ratio of 1:30 g / mL and perform hot reflux extraction. Extract three times, with each extraction lasting 1.5 hours. Combine the extracts, filter, concentrate under reduced pressure to 20 wt%, sterilize, and freeze-dry to obtain the tomato fruit extract.

[0121] Preparation Example 4-3

[0122] This preparation example provides a tomato fruit extract, which is prepared using the following method:

[0123] Wash and crush the tomatoes to make a tomato paste. Mix the paste with an 80% ethanol aqueous solution at a material-to-liquid ratio of 1:10 g / mL and perform hot reflux extraction once for 3 hours. Combine the extracts, filter, concentrate under reduced pressure to 20 wt%, sterilize, and freeze-dry to obtain the tomato fruit extract.

[0124] Example 1

[0125] This embodiment provides a composition comprising, by weight parts: 5 parts of Balkan gesneriaceae leaf extract of Preparation Example 1-1, 5 parts of kangaroo paw extract of Preparation Example 2-1, 5 parts of Indian swert sprout extract of Preparation Example 3-1, 5 parts of tomato fruit extract of Preparation Example 4-1, and 1 part of polydeoxyribonucleotide.

[0126] Example 2

[0127] This embodiment provides a composition comprising, by weight parts: 5 parts of Balkan gesneriaceae leaf extract from Preparation Example 1-2, 1 part of kangaroo paw extract from Preparation Example 2-2, 5 parts of Indian swert sprout extract from Preparation Example 3-2, 1 part of tomato fruit extract from Preparation Example 4-2, and 0.2 parts of polydeoxyribonucleotides.

[0128] Example 3

[0129] This embodiment provides a composition comprising, by weight parts: 1 part of Balkan gesneriaceae leaf extract of Preparation Examples 1-3, 5 parts of kangaroo paw extract of Preparation Examples 2-3, 1 part of Indian swert sprout extract of Preparation Examples 3-3, 5 parts of tomato fruit extract of Preparation Examples 4-3, and 1 part of polydeoxyribonucleotide.

[0130] Examples 4-7

[0131] Examples 4-7 each provide a composition, which differs from Example 1 only in that the Balkan gesneriaceae leaf extract of Preparation Example 1-1 in Example 1 is replaced with equal parts by mass of Balkan gesneriaceae leaf extract of Preparation Example 1-4 to Preparation Example 1-7, while the rest of the formulation remains the same as in Example 1.

[0132] Examples 8-11

[0133] Examples 8-11 each provide a composition, which differs from Example 1 only in that the kangaroo paw extract of Preparation Example 2-1 in Example 1 is replaced with equal parts by mass of kangaroo paw extracts of Preparation Examples 2-4 to 2-7, respectively, while the rest of the formulation remains the same as in Example 1.

[0134] Comparative Example 1

[0135] This comparative example provides a composition comprising, by mass parts: 5 parts of kangaroo paw extract of Preparation Example 2-1, 5 parts of Indian water deer sprout extract of Preparation Example 3-1, 5 parts of tomato fruit extract of Preparation Example 4-1, and 1 part of polydeoxyribonucleotide.

[0136] Comparative Example 2

[0137] This comparative example provides a composition comprising, by mass parts: 5 parts of Balkan gesneriaceae leaf extract of Preparation Example 1-1, 5 parts of Indian swert sprout extract of Preparation Example 3-1, 5 parts of tomato fruit extract of Preparation Example 4-1, and 1 part of polydeoxyribonucleotide.

[0138] Comparative Example 3

[0139] This comparative example provides a composition comprising, by mass parts: 5 parts of Balkan gesneriaceae leaf extract of Preparation Example 1-1, 5 parts of kangaroo paw extract of Preparation Example 2-1, 5 parts of tomato fruit extract of Preparation Example 4-1, and 1 part of polydeoxyribonucleotide.

[0140] Comparative Example 4

[0141] This comparative example provides a composition comprising, by mass parts: 5 parts of Balkan gesneriaceae leaf extract of Preparation Example 1-1, 5 parts of kangaroo paw extract of Preparation Example 2-1, 5 parts of Indian swert sprout extract of Preparation Example 3-1, and 1 part of polydeoxyribonucleotide.

[0142] Comparative Example 5

[0143] This comparative example provides a composition comprising, by mass parts: 5 parts of Balkan gesneriaceae leaf extract of Preparation Example 1-1, 5 parts of kangaroo paw extract of Preparation Example 2-1, 5 parts of Indian swert sprout extract of Preparation Example 3-1, and 5 parts of tomato fruit extract of Preparation Example 4-1.

[0144] Application Example 1

[0145] This application example provides an emulsion comprising the following components:

[0146] Table 2

[0147]

[0148] Preparation process:

[0149] (1) Phase A and Phase B are heated to 80℃ and dissolved completely;

[0150] (2) Phase B is drawn into Phase A, homogenized for 3 min, stirred and cooled to 45°C, component C is added, stirred and cooled to 40°C, and the product is discharged after passing inspection to obtain the emulsion.

[0151] Application Examples 2-11

[0152] Application Examples 2-11 each provide an emulsion, the only difference from Application Example 1 being that the composition of Example 1 is replaced by an equal mass of the compositions of Examples 2-11, while the rest of the formulation and preparation process remain the same as in Application Example 1.

[0153] Comparative Application Examples 1-5

[0154] Comparative Application Examples 1-5 each provide an emulsion, the only difference from Application Example 1 being that the composition of Example 1 is replaced by an equal mass of the compositions of Comparative Examples 1-5, while the rest of the formulation and preparation process remain the same as Application Example 1.

[0155] Test Example 1

[0156] Evaluation of anti-aging efficacy:

[0157] 160 subjects aged 20-45 years were randomly selected, including 80 men and 80 women. The selected subjects presented with fine lines, noticeable wrinkles, and sagging skin. The subjects were divided into 16 groups. After cleansing their skin, each group applied an appropriate amount of the lotion products from Examples 1-11 and Comparison Examples 1-5 evenly to the entire face and eye area, gently massaging until fully absorbed. This was done twice daily, morning and evening, for two consecutive months. Skin images were captured using the VISIA-CR facial image analyzer to measure the proportion of wrinkles, and the reduction rates of crow's feet and wrinkles were calculated.

[0158] Crow's feet reduction rate = (Percentage of crow's feet before using the product - Percentage of crow's feet after using the product) / Percentage of crow's feet before using the product × 100%.

[0159] Nasolabial fold reduction rate = (Nasolabial fold percentage before product use - Nasolabial fold percentage after product use) / Nasolabial fold percentage before product use × 100%.

[0160] The test results are shown in Table 3.

[0161] Table 3

[0162] Group crow's feet reduction rate Nasolabial fold reduction rate Application Example 1 29.44％ 28.56％ Application Example 2 26.59％ 25.39％ Application Example 3 26.96％ 26.34％ Application Example 4 16.31％ 15.91％ Application Example 5 15.20％ 16.22％ Application Example 6 19.01％ 21.56％ Application Example 7 21.18％ 18.02％ Application Example 8 16.13％ 20.04％ Application Example 9 19.96％ 19.73％ Application Example 10 20.08％ 22.55％ Application Example 11 17.31％ 16.71％ Comparative Application Example 1 12.92％ 14.03％ Comparative Application Example 2 13.41％ 10.49％ Comparative Application Example 3 13.63％ 11.17％ Comparative Application Example 4 15.24％ 10.85％ Comparative Application Example 5 12.71％ 14.02％

[0163] As can be seen from the test data in Table 3, the composition of the present invention, which includes Balkan gesneriad leaf extract, Indian swert extract, kangaroo paw extract, tomato fruit extract and polydeoxyribonucleotides, has excellent anti-wrinkle and anti-aging effects.

[0164] A comparison of the data from Application Example 1 and Comparative Application Examples 1-5 shows that when any one of Balkan Gesneria leaf extract, Indian swert extract, kangaroo paw extract, tomato fruit extract, or polydeoxyribonucleotides is missing from the composition, the anti-wrinkle efficacy is significantly reduced, indicating that the above components have a significant synergistic effect in anti-wrinkle.

[0165] A comparison of the data from Application Example 1 and Application Example 4 shows that, in the preparation of Balkan Gesneria leaf extract, pretreatment of the raw materials with ultra-high pressure microfluidic jet can better extract the active ingredients from the plant raw materials, thereby exerting excellent anti-wrinkle effects.

[0166] A comparison of the data from Application Example 1 and Application Examples 5-7 shows that, in the preparation of Balkan gesneriaceae leaf extract, hemicellulase, β-glucosidase, and tanninase have a certain synergistic effect on the enzymatic hydrolysis of Balkan gesneriaceae leaves. Enzymatic hydrolysis of the pretreated solution of Balkan gesneriaceae leaves with this complex enzyme can further improve the anti-wrinkle effect of the prepared Balkan gesneriaceae leaf extract.

[0167] A comparison of the data from Application Example 1 and Application Example 8 shows that microwave extraction of the raw materials during the preparation of kangaroo paw extract can better extract the active ingredients from the plant materials, thereby exerting excellent anti-wrinkle effects.

[0168] A comparison of the data from Application Example 1 and Application Examples 9-11 shows that, in the preparation of kangaroo paw extract, using a combination of chitosan, sodium carboxymethyl starch, and guar gum as flocculants to flocculate the crude kangaroo paw extract can further enhance the enrichment of active ingredients and improve the anti-wrinkle efficacy of the extract.

[0169] Test Example 2

[0170] Firming effect evaluation:

[0171] The same group of subjects from Test Example 1 were selected. This group also underwent the following skin tightening effect test, with each group using the lotion products from Application Examples 1-11 and Control Examples 1-5, respectively. Subjects first cleansed their faces for the same amount of time. After the skin dried, a 5cm × 5cm area of ​​skin on the cheek was selected as the test area. 1mL of the lotion prepared in Application Examples 1-11 and Control Examples 1-5 was evenly applied to the test area on the face. The skin elasticity R² value was measured before use and after 4 weeks of continuous use using a skin elasticity meter (Cutometer MPA580, CK GmbH, Germany). The skin elasticity R² value for each test area was the average of 5 measurements. The closer the skin elasticity R² value is to 1, the better the skin elasticity. The rate of change in skin elasticity was calculated.

[0172] Skin elasticity change rate = (average R2 after product use - average R2 before product use) / average R2 before product use × 100%.

[0173] The test results are shown in Table 4.

[0174] Table 4

[0175] Group Skin elasticity change rate Application Example 1 39.28％ Application Example 2 36.45％ Application Example 3 35.98％ Application Example 4 29.82％ Application Example 5 31.44％ Application Example 6 29.85％ Application Example 7 32.78％ Application Example 8 32.96％ Application Example 9 30.77％ Application Example 10 28.13％ Application Example 11 33.63％ Comparative Application Example 1 11.94％ Comparative Application Example 2 12.45％ Comparative Application Example 3 10.74％ Comparative Application Example 4 16.19％ Comparative Application Example 5 19.54％

[0176] As can be seen from the test data in Table 4, the composition of the present invention, which includes Balkan gesneriad leaf extract, Indian swert extract, kangaroo paw extract, tomato fruit extract and polydeoxyribonucleotides, has excellent firming effects.

[0177] A comparison of the data from Application Example 1 and Comparative Application Examples 1-5 shows that when any one of Balkan Gesneria leaf extract, Indian swert extract, kangaroo paw extract, tomato fruit extract, or polydeoxyribonucleotides is missing from the composition, the firming effect is significantly reduced, indicating that the above components have a significant synergistic effect in improving skin firmness.

[0178] A comparison of the data from Application Example 1 and Application Example 4 shows that, in the preparation of Balkan Gesneriaceae leaf extract, pretreatment of the raw materials with ultra-high pressure microfluidic jets can better extract the active ingredients from the plant materials, thereby achieving excellent firming effects.

[0179] A comparison of the data from Application Example 1 and Application Examples 5-7 shows that, in the preparation of Balkan gesneriad leaf extract, hemicellulase, β-glucosidase, and tanninase have a certain synergistic effect on the enzymatic hydrolysis of Balkan gesneriad leaves. Using this complex enzyme to enzymatically hydrolyze the pretreated solution of Balkan gesneriad leaves can further improve the firming effect of the prepared Balkan gesneriad leaf extract.

[0180] A comparison of the data from Application Example 1 and Application Example 8 shows that microwave extraction of the raw materials during the preparation of kangaroo paw extract can better extract the active ingredients from the plant materials, thereby achieving excellent firming effects.

[0181] A comparison of the data from Application Example 1 and Application Examples 9-11 shows that, in the preparation of kangaroo paw extract, using a combination of chitosan, sodium carboxymethyl starch, and guar gum as flocculants to flocculate the crude kangaroo paw extract can further enhance the enrichment of active ingredients and improve the firming effect of the extract.

[0182] The applicant declares that the technical solution of this invention is illustrated by the above embodiments, but this invention is not limited to the above embodiments, that is, it does not mean that this invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the products of this invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of this invention.

[0183] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0184] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A composition having anti-wrinkle and firming effects, characterized in that, The composition comprises Balkan gesneriad leaf extract, Indian swert extract, kangaroo paw extract, tomato fruit extract, and polydeoxyribonucleotides.

2. The composition according to claim 1, characterized in that, The composition comprises, by weight parts, 1-5 parts of Balkan gesneriad leaf extract, 1-5 parts of Indian swert sprout extract, 1-5 parts of kangaroo paw extract, 1-5 parts of tomato fruit extract, and 0.2-1 parts of polydeoxyribonucleotides.

3. The composition according to claim 1, characterized in that, The Balkan gesneriaceae leaf extract was prepared using a method comprising the following steps: (1) Balkan gesneriaceae leaf raw material was mixed with water and subjected to ultra-high pressure micro-jet pretreatment to obtain pretreated solution; (2) The pretreatment solution was mixed with the compound enzyme to carry out the enzymatic hydrolysis reaction to obtain the enzymatic hydrolysate. The enzymatic hydrolysate was filtered and the filtrate was collected to obtain Balkan gesneriaceae leaf extract.

4. The composition according to claim 3, characterized in that, In step (1), the ratio of Balkan gesneriaceae leaf raw material to water is 1:(20-40)g / mL; The temperature of the ultra-high pressure microjet pretreatment in step (1) is 50-70℃, the pressure of the ultra-high pressure microjet pretreatment is 100-400MPa, and the number of ultra-high pressure microjet pretreatments is 1-4 times.

5. The composition according to claim 3, characterized in that, The complex enzyme in step (2) includes hemicellulase, β-glucosidase and tanninase; The mass ratio of hemicellulase, β-glucosidase, and tanninase is (1-5):(5-10):(0.1-0.5); The mass ratio of the pretreatment solution to the compound enzyme in step (2) is 100:(1-5); The temperature of the enzymatic hydrolysis reaction in step (2) is 40-60℃, and the reaction time is 0.5-2h.

6. The composition according to claim 1, characterized in that, The kangaroo paw extract was prepared using a method comprising the following steps: (I) Kangaroo paw raw material was mixed with water and microwaved for extraction. The filtrate was collected by filtration to obtain crude kangaroo paw extract. (II) The crude extract of kangaroo paw was mixed with a flocculant for flocculation treatment, and the supernatant was collected by centrifugation to obtain the kangaroo paw extract.

7. The composition according to claim 6, characterized in that, In step (I), the ratio of kangaroo paw raw material to water is 1:(30-60)g / mL; The microwave extraction temperature in step (I) is 50-80℃, the microwave extraction power is 200-500W, and the microwave extraction time is 10-30min; The flocculant in step (II) includes chitosan, sodium carboxymethyl starch, and guar gum; The mass ratio of chitosan, sodium carboxymethyl starch and guar gum is 1:(0.5-1):(1-3); The mass ratio of the crude kangaroo paw extract to the flocculant in step (II) is 100:(0.1-2).

8. The composition according to claim 1, characterized in that, The Indian swert extract was prepared using a method comprising the following steps: The raw material of Indian swert was mixed with water and subjected to hot extraction. The supernatant was collected by centrifugation to obtain Indian swert extract.

9. The composition according to claim 1, characterized in that, The tomato fruit extract was prepared using a method comprising the following steps: The tomato fruit raw material was mixed with an aqueous solution of ethanol and subjected to hot reflux extraction. The filtrate was collected by filtration to obtain tomato fruit extract.

10. The use of a composition having anti-wrinkle and firming effects as described in any one of claims 1-9 in cosmetics.

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