Novel vegetable health magnesium resistance inducer as well as preparation method and application thereof

Abstract

The invention discloses a novel vegetable health magnesium resistance inducer as well as a preparation method and application thereof, the main components of the vegetable health magnesium resistance inducer are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), and the components have the characteristics of no toxicity, harmlessness, no pollution and the like. The invention finds that the'vegetable health magnesium 'resistance inducer can activate plant hormone signals, enhance plant immunity and resist virus diseases. The composition can be sprayed to vegetable crops before the high-incidence period of vegetable virus diseases to prevent and treat virus diseases, is simple and convenient, has strong operability, and can effectively improve the broad-spectrum resistance of plants to various vegetable virus diseases. Compared with traditional pesticides, the composition is more green and safer, meets the requirements of sustainable and healthy development of agriculture, and has huge application potential.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of biological pesticides, and particularly relates to preparation and application of a novel "Caijianmagnesium" elicitor. BACKGROUND

[0002] Plants are often attacked by various plant pathogens such as bacteria, fungi, oomycetes and viruses during growth and development. These plant pathogens can cause serious plant diseases. Among them, plant viruses are the main cause of plant diseases, causing about 30 billion US dollars of economic loss in global agricultural production every year, and plant viral diseases are also known as "plant cancer". At present, the main viruses that harm vegetable crops in China include tobacco mosaic virus (TMV), turnip mosaic virus (TuMV) and cucumber mosaic virus (CMV). So far, although there are many methods to control viral diseases, such as chemical control, biological control and breeding of disease-resistant varieties, viral diseases are still a difficult point in plant disease control. In particular, the widespread use of chemical pesticides has led to many environmental and social problems, such as threats to human health, pesticide residues in food, destruction of agricultural biodiversity and ecological balance, and development of pest and pathogen resistant strains. Therefore, in order to maintain the sustainable development of agriculture, it is urgent to develop modern, environmentally friendly and sustainable viral disease control strategies that are safe for human health and the environment. Plant immune elicitors can induce plants to actively produce resistance to pests and diseases, providing new ideas for improving crop production quality and promoting sustainable crop development. Immune induction technology based on immune elicitors has the characteristics of broad-spectrum, durability, stability, safety and the like, and activates plant immune defense against pathogenic bacteria, which has attracted widespread attention. At present, some substances that induce plant immunity have been developed into immune elicitors, such as ZhiNengCong (ZNC), Benzothiadiazole (BTH) and Methiadinil (thiazide induced amine), which are applied in the prevention and control of diseases.

[0003] Trimethylglycine (GB) is a kind of natural zwitterionic quaternary ammonium water-soluble alkaloid existing in plants, which is an important osmoprotectant for maintaining normal physiological functions of plants, and participates in plant growth and development, secondary metabolism and defense response to abiotic stress. Nanomaterials have excellent physical and chemical properties, and as new nanometer agricultural chemicals for preventing and controlling plant diseases, they have attracted special attention. Among them, magnesium oxide nanoparticles (MgONPs) are a kind of non-toxic and relatively easy to obtain inorganic metal oxides, which have been identified as safe materials by the US Food and Drug Administration. However, whether GB and MgONPs can synergistically induce plant immunity to enhance plant resistance to viral infection is unknown. SUMMARY

[0004] Invention purposes: In view of the problems existing in the prior art, the application provides a new vegetable health magnesium elicitor which can be used for inducing immune vegetable virus diseases, the application develops the GB and MgONPs into the vegetable health magnesium elicitor by compounding, and the elicitor can synergistically induce plant immunity and enhance the broad-spectrum resistance of plants to various vegetable virus diseases, so as to solve a series of problems such as unsatisfactory prevention and treatment effect, drug resistance, pesticide residue and food safety in the current virus disease prevention and treatment measures.

[0005] The application further provides a preparation method and application of the new vegetable health magnesium elicitor.

[0006] Technical scheme: In order to achieve the above-mentioned purposes, the application provides a new vegetable health magnesium elicitor, the main components of the vegetable health magnesium elicitor are GB and MgONPs, and the mass ratio of the GB and MgONPs is 10-20:1.

[0007] Preferably, the mass ratio of the GB and MgONPs is 15-16:1.

[0008] Preferably, the mass ratio of the GB and MgONPs is 15.6:1.

[0009] Preferably, the elicitor further comprises an auxiliary agent.

[0010] Preferably, the auxiliary agent comprises any one or more of wetting agents, dispersants, thickening agents and antifreezing agents.

[0011] Preferably, the wetting agent is selected from one of GY-WS10, Morwet EFW and GY-WS03; the dispersant is selected from one of GY-D800 and GY-009; the thickening agent is selected from one of xanthan gum and magnesium aluminum silicate; and the antifreezing agent is selected from one of ethylene glycol and propylene glycol.

[0012] The preparation method of the new vegetable health magnesium elicitor provided by the application comprises the following steps:

[0013] The GB and MgONPs are dissolved in water and uniformly mixed, and then an auxiliary agent dispersant, a thickening agent, a wetting agent and an antifreezing agent are added and cut by a high-speed shearing dispersion emulsifier for 10 minutes, and finally the bubbles in the liquid are removed by an ultrasonic cleaning machine.

[0014] The application of the new vegetable health magnesium elicitor in enhancing plant immunity and disease resistance.

[0015] Preferably, the elicitor enhances plant immunity and disease resistance through at least one of the following (1)-(3) signal pathways:

[0016] (1) promoting plant growth and development;

[0017] (2) Activate the salicylic acid, jasmonic acid and ethylene defense signaling pathways;

[0018] (3) Alleviate virus-induced oxidative damage.

[0019] The plant is a vegetable or tobacco, including vegetables such as tomato, turnip, and potato, and the disease-resistant virus includes tobacco mosaic virus (TMV), tomato mosaic virus (ToMV), turnip mosaic virus (TuMV), potato virus X (PVX), potato virus Y (PVY), and tomato spotted wilt virus (TSWV).

[0020] The inducer is sprayed onto the leaves of the plant before the plant becomes diseased.

[0021] This invention combines GB and MgONPs to synergistically induce plant immunity to defend against vegetable viral diseases, and screens out the most suitable adjuvants (dispersants, wetting agents, thickeners, and antifreeze agents, etc.) to prepare a suspension. This invention is the first to discover that GB can induce plant immunity to defend against viral infection. To further improve the disease resistance effect of GB, it combines GB with other substances that can induce plant immunity (γ-aminobutyric acid, glutathione, and magnesium oxide nanoparticles). External application to plants revealed the best combination for disease resistance: GB and MgONPs, with significantly higher efficacy than other combinations of substances that induce plant immunity with GB. Then, considering both disease resistance effect and economic cost, a specific ratio of GB and MgONPs was selected to develop the "Caijian Magnesium" resistance inducer, and the mechanism by which the "Caijian Magnesium" resistance inducer defends against viral infection was studied.

[0022] In this invention, GB and MgONPs are combined to synergistically enhance resistance to various vegetable viral diseases, and the most economical and efficient ratio is selected while ensuring the preventive effect.

[0023] This invention is the first to discover that GB can induce plant immunity against viral infection, revealing that GB and MgONPs simultaneously activate the SA, JA, and ET signaling pathways of plant hormones to enhance plant immunity. It is also the first to develop a compound of GB and MgONPs into a "Caijian Magnesium" resistance inducer. Compared with existing chemical pesticides for viral infection prevention, the "Caijian Magnesium" inducer solves a series of problems in current viral disease control measures, such as unsatisfactory control effects, pesticide resistance, pesticide residues, and food safety, by improving the plant's own immunity. Results from this invention show that GB and MgONPs at concentrations of 20 mM (2.34 mg / ml) and 150 μg / mL have significant resistance effects, and are significantly superior to GB or MgONPs alone.

[0024] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0025] 1、The "vegetable health magnesium" induced resistance agent in the application has synergistic effect of GB and MgONPs, and the effect of improving plant disease resistance is significantly higher than that of using single component, so that the broad-spectrum resistance of plants to various vegetable viral diseases is effectively improved, and important guarantee is provided for sustainable management of vegetable viral diseases, quality safety, ecological safety of agricultural products, and sustainable development of vegetable production, and the purpose of reducing drugs and developing agriculture is achieved.

[0026] 2、The "vegetable health magnesium" induced resistance agent in the application starts from improving the immunity of plants, and compared with the traditional chemical control method, it is more green and safe, so that important guarantee is provided for quality safety, ecological safety of agricultural products, and sustainable development of vegetable production, and the traditional chemical control is promoted to modern green control.

[0027] 3、The "vegetable health magnesium" induced resistance agent in the application can prevent and control various vegetable viral diseases, such as tobacco mosaic virus (TMV), tomato mosaic virus (ToMV), turnip mosaic virus (TuMV), potato X virus (PVX), potato Y virus (PVY) and tomato spotted wilt virus (TSWV), so that it has high broad-spectrum resistance.

[0028] 4、The preparation process of the application is simple and easy to operate, the production cost is low, the production process is easy to control, and it is beneficial to large-scale factory production and field application, and easy to popularize and apply.

[0029] 5、The "vegetable health magnesium" induced resistance agent in the application needs only 4.8 yuan per mu, and the prevention effect is as high as 65%, compared with other immune induced resistance agents on the market, the cost is reduced by more than 10%, the prevention effect is improved by more than 15%, and the economic efficiency is better realized, and the market competitiveness is greatly enhanced. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 The figure is a resistance concentration screening diagram of the "vegetable health magnesium" induced resistance agent, wherein A and B are ultraviolet fluorescence diagrams of TMV resistance of different concentrations of GB and MgONPs respectively, C and D are corresponding imageJ fluorescence area analysis diagrams, and the results show that the resistance effects of 2.34 mg / ml and 150 μg / mL concentrations of GB and MgONPs are best.

[0031] Figure 2Resistance detection of the optimal concentration of GB / MgONPs applied externally; A and F are the UV fluorescence images of GB and MgONPs on the inoculated leaves and systemic leaves, respectively, B, C, D and E are the imageJ fluorescence area analysis images and the RNA expression level of TMV of GB on the inoculated leaves and systemic leaves, and G, H, I and J are the imageJ fluorescence area analysis images and the RNA expression level of TMV of MgONPs on the inoculated leaves and systemic leaves. The results show that the application of GB / MgONPs significantly reduces the content of virus, not only improves the local immunity of plants, but also significantly improves the immunity of the whole plant.

[0032] Figure 3 Effect of GB / MgONPs applied externally on the oxidative damage of TMV-induced tobacco; A, B, C and D are the conductivity level and malondialdehyde accumulation in tobacco leaves before and after GB / MgONPs treatment before TMV-GFP inoculation; E and F are the effect of GB / MgONPs applied externally on the active oxygen signal, and the content of ROS in tobacco leaves is detected by NBT and DAB staining method. The results show that the application of GB / MgONPs reduces the oxidative damage caused by the accumulation of active oxygen induced by TMV.

[0033] Figure 4 Effect of GB / MgONPs applied externally on the SA, JA and ET hormone signal pathways of plants; A is that the application of GB up-regulates the expression of SA-related genes, mainly activating the SA signal pathway; B is that the application of MgONPs up-regulates the expression of SA, JA and ET-related genes, activating the SA, JA and ET signal pathways.

[0034] Figure 5 Application of GB / MgONPs can promote the growth and development of plants; A and G are the phenotype of tobacco growth after the application of GB / MgONPs; B and H detect the root length; C and I detect the plant height; D and J detect the leaf area; E and K detect the fresh weight; F and L detect the dry weight. The results show that GB and MgONPs significantly promote the growth of tobacco, and the best growth-promoting effect is obtained by 2.34 mg / ml of GB and 250 μg / mL of MgONPs.

[0035] Figure 6The detection of the broad-spectrum resistance of the vegetable crops to the viral diseases induced by the external application of the GB / MgONPs / GB+MgONPs compound solution; A-F: the external application of the GB / MgONPs / GB+MgONPs compound solution on the tobacco plants improves the broad-spectrum resistance to ToMV, TuMV, PVX, TMV, ToMMV and PVY; G-K: the external application of the GB / MgONPs / GB+MgONPs compound solution on the tomato plants improves the broad-spectrum resistance to TMV, PVX, PVY, ToMV and TuMV. Among them, the external application of the GB+MgONPs compound solution has the best broad-spectrum resistance effect on the viral diseases of the vegetables.

[0036] Figure 7 The application of the broad-spectrum viral resistance of the "Caijianmagnesium" elicitor. The water and the "Caijianmagnesium" elicitor are sprayed on the tobacco and tomato, and then ToMV-GFP, TuMV-GFP, PVX-GFP, PVY-GFP and TMV-GFP are injected and rubbed into the leaves, respectively. It is observed under the long-wave ultraviolet lamp that, after the external application of the "Caijianmagnesium" elicitor, the fluorescence area of the inoculation leaves and the systemic leaves inoculated with different viruses is significantly reduced compared with the control. And it is observed under the fluorescence microscope that, compared with the control, the fluorescence intensity of different viruses in the leaves externally applied with the "Caijianmagnesium" elicitor is also significantly reduced. These results show that the "Caijianmagnesium" elicitor exhibits good broad-spectrum antiviral effect in different crops. DETAILED DESCRIPTION

[0037] The application is further described below in combination with the drawings and examples.

[0038] The experimental methods described in the examples are all conventional methods unless otherwise specified; and the reagents and materials described are all commercially available unless otherwise specified.

[0039] The trimethylglycine (GB) in the examples is purchased from Shenguo Bioengineering (Shanghai) Co., Ltd.; and the magnesium oxide nanoparticles (MgONPs) is purchased from Shanghai Maikelin Biochemical Co., Ltd., with the product number M813080, 99.99% metals basis, and the average particle size: 50 nm.

[0040] Tobacco mosaic virus (TMV), tomato mosaic virus (ToMV), turnip mosaic virus (TuMV), potato virus X (PVX), potato virus Y (PVY) and tomato spotted wilt virus (TSWV), the above viruses carrying GFP are all provided by Yangzhou University. TMV-GFP is inoculated by rubbing with quartz sand, and the rest of the viruses with GFP are injected after adjusting the OD600 value to 0.6 by spectrophotometer.

[0041] Example 1

[0042] GB is directly dissolved in water, MgONPs is dissolved in water and needs ultrasonic treatment for 5 minutes. Four-week-old tobacco plants are selected, and the tobacco plants with good growth and uniform size are sprayed. The leaves are uniformly sprayed and can be used immediately.

[0043] In order to explore whether GB / MgONPs affect the resistance response of tobacco to TMV infection, 0 mg / ml, 0.58 mg / ml, 1.17 mg / ml, 2.34 mg / ml of GB and 0 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL, 200 μg / mL, 250 μg / mL of MgONPs are set to spray tobacco leaves, and 24 hours later, quartz sand rubbing method is used to inoculate TMV-GFP (10 μL of TMV-GFP is inoculated per leaf), and green fluorescence is directly observed by long-wave ultraviolet lamp. As shown in Figure 1 The results show that among several concentrations, 20 mM GB (2.34 mg / ml) and 150 μg / mL MgONPs treated plants show the best inhibitory effect, so this concentration is used in subsequent studies. At the same time, the ratio of leaf virus fluorescence is calculated by Image J software, and similar conclusions are obtained.

[0044] In subsequent examples, the reagent composed of 20 mM GB (2.34 mg / ml) and 150 μg / mL MgONPs is used as a "Caijianmagnesium" elicitor.

[0045] Further, 20 mM GB (2.34 mg / ml) and 150 μg / mL MgONPs can be used, and auxiliary dispersants, thickeners, wetting agents, and antifreeze agents are added and cut by a high-speed shearing dispersion emulsifier for 10 minutes. Finally, it is put into an ultrasonic cleaner to remove bubbles in the liquid to prepare the preparation. The wetting agent is selected from one of GY-WS10, Morwet EFW, and GY-WS03; the dispersant is selected from one of GY-D800 and GY-009; the thickener is selected from one of xanthan gum and magnesium aluminum silicate; and the antifreeze agent is selected from one of ethylene glycol and propylene glycol.

[0046] Example 2

[0047] In order to further verify the experimental results of concentration screening, 20 mM GB / 150 μg / mL MgONPs are used to treat tobacco according to the method of Example 1, and the treatment method is the same as that of Example 1, and similar conclusions are obtained. In addition, the RNA level of TMV in inoculated leaves and systemic leaves is detected by real-time fluorescent quantitative PCR, as shown in Figure 2As shown, the RNA level of TMV was significantly decreased after the application of GB or MgONPs. This indicated that the application of GB / MgONPs could enhance the immunity of tobacco to virus infection, and the application of GB / MgONPs significantly reduced the content of virus, not only improved the local immunity of the plant, but also significantly improved the immunity of the whole plant.

[0048] Example 3

[0049] To explore the relationship between intracellular oxidative damage and the resistance of N. benthamiana induced by GB / MgONPs, the conductivity level and the content of malondialdehyde were detected. The tobacco was treated according to the optimal concentration of GB and MgONPs and the method of Example 1, and the leaves before and after inoculation of TMV-GFP were cut into pieces and put into test tubes after 24h of GB or MgONPs treatment, and deionized water was added for vacuum infiltration for 20 minutes. After standing at room temperature for 1 hour, the solution conductivity Ri was measured with a conductivity meter. The test tube was placed in a 100℃ water bath for 5 minutes, and after the test tube cooled to room temperature, it was diluted to 5ml, and the solution conductivity Rt was measured again. The relative conductivity Ro was calculated using the formula Ro=Ri / Rt. The content of malondialdehyde (MDA) was detected by using the MDA content detection kit: 0.1g of plant tissue was placed in a sterilized mortar, and liquid nitrogen was added for rapid grinding to powder and placed in a 1.5ml sterile EP tube, 1ml of extraction solution was added and mixed, 4℃ refrigerated centrifuge 12000rpm for 10min, the supernatant was taken into a new centrifuge tube and used. Take 100μL of supernatant into a new centrifuge tube, and add MDA detection working solution and other reagents, mix well, then react in a 100℃ water bath for 45min, then ice bath for 5min, after cooling, centrifuge at 4℃ in a refrigerated centrifuge at 12000rpm for 10min. Take part of the supernatant into a 1ml cuvette, detect and record the OD value with a visible spectrophotometer.

[0050] As Figure 3As shown, the results showed that the conductivity level and the malondialdehyde content were low when no TMV was inoculated, and the conductivity level and the malondialdehyde content increased after 3 days of inoculation of TMV-GFP, and the conductivity level and the malondialdehyde content in the treatment group of exogenous GB / MgO NPs were significantly lower than those in the control group. The results showed that exogenous GB / MgO NPs had no significant effect on the cell membrane in N. benthamiana, but could reduce the conductivity level and the accumulation of malondialdehyde induced by TMV infection, and alleviate the cell membrane damage induced by TMV infection. In order to explore the effect of exogenous GB / MgO NPs on the active oxygen signal, the content of ROS in tobacco leaves was detected by NBT and DAB staining method. When no TMV was inoculated, compared with CK, exogenous GB had no effect on the accumulation of ROS, while exogenous MgO NPs promoted the increase of ROS; after inoculation of TMV, exogenous GB / MgO NPs reduced the accumulation of ROS, indicating that exogenous GB / MgO NPs could alleviate the oxidative stress caused by ROS induced by TMV infection.

[0051] Example 4

[0052] In order to explore whether the exogenous GB / MgO NPs enhance the disease resistance of N. benthamiana by regulating the molecular signal mechanism of hormone signal mediated by salicylic acid (SA), jasmonic acid (JA) or ethylene (ET), the contents of SA, JA and ET in tobacco treated with water and GB / MgO NPs were detected, and the expression amount of hormone synthesis and related defense genes was observed. Hormone content detection: the tobacco was treated according to the optimal GB and MgO NPs concentration and method of example 1, and the leaves treated with GB or MgO NPs and control water for 24 h were ground into powder with liquid nitrogen, 0.5 g powder was taken and 500 μL extraction solvent was added. Under the condition of 4°C and 100 r / min, vibration for 30 min. 1 mL dichloromethane was added to each test tube, and centrifuged at 4°C and 13,000 r / min for 5 min. After centrifugation, two phases were formed, and the plant fragments were between the two layers. About 900 μL of the lower phase was taken with a pipette and transferred to a new centrifuge tube, and the sample was freeze-dried and redissolved in 100 μL of methanol. After obtaining the plant hormones, the contents of SA, JA and ET in tobacco leaves were detected by double antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA), and the operation steps were referred to the plant ELISA kit of Shanghai Qidu Biological Technology Co., Ltd. Detection of expression amount of hormone synthesis and related defense genes: the tobacco was treated according to the optimal GB or MgO NPs concentration and method of example 1, and the leaves treated with GB or MgO NPs and control water for 24 h were ground into powder with liquid nitrogen, and the plant RNA was extracted by Novizen RNA extraction kit, and then the RNA was reverse transcribed into cDNA by full-form gold TransScript reverse transcription kit, and the expression amount of related genes was detected by RT-qPCR. For example Figure 4As shown in the results, compared with the control, the exogenous application of GB significantly induced the up-regulation of the expression of SA-related hormone synthesis gene NbICS1, related hormone receptor NbNPR1, related defense genes NbPR1, NbPR2 and NbPR5; the expression of JA and ET related pathway genes had no statistical significance. Therefore, the disease resistance induced by the exogenous application of GB was probably mediated by the activation of the SA signaling pathway. Compared with the control, the exogenous application of MgONPs significantly up-regulated the contents of endogenous hormones SA, JA and ET in plants, and induced the up-regulation of the expression of SA-related hormone synthesis gene NbICS1, related hormone receptor NbNPR1, related defense genes NbPR1, NbPR2 and NbPR5, JA-related hormone synthesis gene NbOPR3, defense gene NbPDF1.2, NbPR3 and related hormone receptor NbCOI1, and ET-related hormone receptor NbEIN2, related defense gene NbPR4 and related hormone synthesis gene NbACCOx. It was indicated that the disease resistance induced by the exogenous application of MgONPs was probably mediated by the activation of the SA, JA and ET signaling pathways.

[0053] Example 5

[0054] Tobacco plants were treated with different concentrations of GB (0 mg / ml, 0.58 mg / ml, 1.17 mg / ml and 2.34 mg / ml) and MgONPs (0 μg / ml, 100 μg / ml, 150 μg / ml and 250 μg / ml). After the solutions of different concentrations were prepared, they were uniformly sprayed on the leaves of the plants. After 14 days, the tobacco plants were harvested to measure the root length, plant height, leaf area and fresh weight. The dry weight was measured as follows: the harvested tobacco plants were placed in an 80-degree oven overnight (12 h) to dry and then the dry weight was measured. As shown in FIG. 2, it was found that GB and MgONPs significantly promoted the growth of tobacco, especially 2.34 mg / ml of GB and 250 μg / mL of MgONPs. Then, by detecting the physiological parameters such as root length, plant height, leaf area, fresh weight and dry weight, the same results were obtained, and the growth-promoting effect of 2.34 mg / ml of GB and 250 μg / mL of MgONPs was the best. Figure 5

[0055] Example 6

[0056] Tobacco plants growing for about 4 weeks and tomatoes growing for about 8 weeks were selected. The leaves of the tobacco and tomatoes with good growth and uniform size were selected for spraying. The optimal concentrations of GB and MgONPs in Example 1 were selected for treatment.

[0057] Water, MgONPs S , GB and MgONPs S ​+GB compound solution, after 24 hours, then inject ToMV-GFP, TuMV-GFP, PVX-GFP, ToMMV, and PVY-GFP into the plant leaves (filling the entire leaf), and observe under a long-wave ultraviolet lamp. Figure 6 As shown, after external application of MgONPs, GB, and MgONPs+GB compound solutions, the fluorescence area of ​​inoculated leaves and systemic leaves inoculated with different viruses was significantly reduced compared with the control. Among them, the fluorescence area after treatment with MgONPs+GB compound solution was the smallest. Furthermore, under a fluorescence microscope, it was observed that compared with the control, external application of MgONPs... S GB and MgONP S The fluorescence intensity of leaves treated with MgONPs+GB was also significantly reduced, with the lowest fluorescence intensity observed after treatment with the MgONPs+GB compound solution. These results indicate that external application of MgONPs... S GB and MgONPs+GB compound solutions increased the resistance of tobacco and tomatoes to different viral diseases. Among them, MgONPs... S The +GB compound solution showed the best effect, demonstrating significantly better resistance to various vegetable viral diseases compared to applying GB and MgONPs alone.

[0058] Example 7

[0059] Application of "Caijian Magnesium" Inducer

[0060] Following the method in Example 6, water and the "Caijian Magnesium" inducer were sprayed onto tomatoes and tobacco, respectively. After 24 hours, ToMV-GFP, TuMV-GFP, PVX-GFP, PVY-GFP, and TMV-GFP were injected into the leaves via friction inoculation. Figure 7 As shown, observations under long-wave ultraviolet light revealed that the fluorescence area of ​​inoculated leaves and systemic leaves inoculated with different viruses was significantly reduced compared to the control after external application of the "Caijian Magnesium" induced resistance agent. Fluorescence microscopy also showed a significant decrease in the fluorescence intensity of different viruses in leaves treated with the "Caijian Magnesium" induced resistance agent. These results indicate that the "Caijian Magnesium" induced resistance agent exhibits good broad-spectrum resistance in various crops.

Claims

1. A novel "Caijian Magnesium" antagonist, characterized in that, The main components of the "Caijian Magnesium" antagonist are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), with a mass ratio of GB to MgONPs of 10-20:

1.

2. The novel "Caijian Magnesium" antagonist according to claim 1, characterized in that, The preferred mass ratio of GB to MgONPs is 15-16:

1.

3. The novel "Caijian Magnesium" antagonist according to claim 1, characterized in that, The immune inducer also includes adjuvants.

4. The novel "Caijian Magnesium" antagonist according to claim 2, characterized in that, The additives include any one or more of wetting agents, dispersants, thickeners, and antifreeze agents.

5. The novel "Caijian Magnesium" antagonist according to claim 2, characterized in that, The wetting agent is selected from one of GY-WS10, Morwet EFW and GY-WS03; the dispersant is selected from one of GY-D800 and GY-009; the thickener is selected from one of xanthan gum and magnesium aluminum silicate; and the antifreeze is selected from one of ethylene glycol and propylene glycol.

6. A method for preparing the novel "Caijian Magnesium" antagonist as described in claim 1, characterized in that, Includes the following steps Trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs) were dissolved in water and mixed. Then, additives such as dispersants, thickeners, wetting agents, and antifreeze were added and the mixture was cut using a high-speed shear dispersion emulsifier. Finally, the mixture was placed in an ultrasonic cleaner to remove air bubbles from the liquid.

7. The application of the novel "Caijian Magnesium" resistant inducer as described in claim 1 in enhancing plant immunity and disease resistance.

8. The application according to claim 7, characterized in that, The inducer enhances plant immunity and disease resistance through at least one of the following signaling pathways (1)-(3): (1) Promotes plant growth and development; (2) Activate the salicylic acid, jasmonic acid and ethylene defense signaling pathways; (3) Alleviate virus-induced oxidative damage.

9. The application according to claim 7, characterized in that, The plant is a vegetable or tobacco, and the vegetables include tomato, turnip, and potato. The disease-resistant viruses include tobacco mosaic virus (TMV), tomato mosaic virus (ToMV), turnip mosaic virus (TuMV), potato virus X (PVX), potato virus Y (PVY), and tomato spotted wilt virus (TSWV).

10. The application according to claim 7, characterized in that, The inducer is sprayed onto the leaves of the plant before the plant becomes diseased.

Images