Full-automatic peaceful treatment equipment with carbon dioxide recycling function

The design of the fully automated euthanasia treatment equipment enables the recycling of CO2 and rapid anesthesia, solving the problem that existing equipment cannot accurately control CO2 recycling and rapid anesthesia. This improves the automation level and safety of the equipment, and reduces CO2 emissions and operating costs.

CN120959937APending Publication Date: 2025-11-18ZHEJIANG UNIV
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Patent Information

Application Number
CN202511371625.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-24
Publication Date
2025-11-18

AI Technical Summary

Technical Problem

Existing euthanasia equipment cannot accurately control the recycling of carbon dioxide and achieve closed-loop fully automatic control, resulting in the inability to recycle CO2 and the inability to quickly achieve the anesthetic and lethal effect.

Method used

A fully automated euthanasia treatment device was designed, comprising a frame, a first chamber, a second chamber, a gas storage tank, a pressurized conveying fan, an exhaust fan, a CO2 solubility sensor, and a controller. Through pre-vacuum treatment, CO2 gas recycling, and real-time monitoring, the device ensures that the CO2 solubility and pressure are within a safe range, enabling the reuse of CO2 and rapid anesthesia.

Benefits of technology

It enables the reuse of CO2, reduces gas emissions, shortens the time of animal anesthesia and asphyxiation, ensures operational safety and animal welfare, and improves the automation and safety of operations.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses full-automatic peaceful treatment equipment with a carbon dioxide recycling function. The full-automatic peaceful treatment equipment comprises a rack; the first cabin and the second cabin are arranged on the rack; the first cabin is communicated with the air storage tank through a second valve, and the second cabin is communicated with the air storage tank through a third valve; one end of the pressurized conveying fan is connected with the gas storage tank through a fourth valve, and the other end of the pressurized conveying fan is connected with the first cabin and the second cabin through a second valve and a third valve; the first cabin is connected with the exhaust fan through a fifth valve, the second cabin is connected with the exhaust fan through a sixth valve, and the air storage tank is connected with the exhaust fan through a seventh valve. According to the method, a forevacuum mode is adopted, CO2 replacement is carried out after air is rapidly emptied, the time for anesthesia suffocation of animals is shortened, the peaceful time and the CO2 solubility and dosage are accurately controlled, and animal welfare is effectively guaranteed.
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Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of animal experiments, and particularly relates to a full-automatic euthanasia device with carbon dioxide recycling. BACKGROUND

[0002] Experimental animals play an irreplaceable important role in the field of life sciences, medicine, pharmacy and other biology-related fields, and make important contributions to the continuous breakthroughs in life sciences and medicine. As one of the indispensable devices for animal welfare, the euthanasia device develops towards higher standard environmental emission control, operation efficiency and animal welfare, and automation and energy saving and emission reduction become new trends of development.

[0003] With the development of biomedical research and the growth of scientific research team, the use of experimental animals is increasing, and experimental animals play an important role in scientific research. Therefore, after the experiment is completed, how to euthanize experimental animals in a humane way under the welfare rights and interests of experimental animals has become a key animal welfare problem. Rapid and effective euthanasia of experimental animals in the process of euthanasia can be quiet and painless, and the method of carbon dioxide anesthesia is usually used to achieve the purpose of euthanasia. That is, by filling high solubility carbon dioxide gas into the euthanasia sealed cabin, the solubility of the animal is quickly reached to lose consciousness and awareness until death, so as to achieve the purpose of euthanasia without pain and pain.

[0004] Although carbon dioxide anesthesia has the advantages of convenience and low cost, however, the existing euthanasia device cannot accurately control the euthanasia link, and cannot realize carbon dioxide recycling and closed-loop automatic control. Therefore, through the above analysis, the defects of the prior art are that CO2 cannot be recycled, and the effect of rapid anesthesia to death after vacuum cannot be controlled. SUMMARY

[0005] In view of the problems existing in the prior art, the present application provides a full-automatic euthanasia device with carbon dioxide recycling.

[0006] A full-automatic euthanasia device with carbon dioxide recycling, comprising:

[0007] a rack;

[0008] a first cabin and a second cabin arranged on the rack;

[0009] a gas storage tank, the first cabin is communicated with the gas storage tank through a second valve, and the second cabin is communicated with the gas storage tank through a third valve;

[0010] A pressurized conveying fan, one end of the pressurized conveying fan is connected with the gas storage tank through a fourth valve, the other end of the pressurized conveying fan is connected with the first cabin and the second cabin through the second valve and a third valve;

[0011] An exhaust fan, the first cabin is connected with the exhaust fan through a fifth valve, the second cabin is connected with the exhaust fan through a sixth valve, and the gas storage tank is connected with the exhaust fan through a seventh valve;

[0012] A first CO2 concentration sensor and a first pressure sensor arranged in the first cabin;

[0013] A second CO2 concentration sensor and a second pressure sensor arranged in the second cabin;

[0014] A third CO2 concentration sensor and a third pressure sensor arranged in the gas storage tank;

[0015] A controller connected with the first CO2 concentration sensor, the first pressure sensor, the second CO2 concentration sensor, the second pressure sensor, the third CO2 concentration sensor, the third pressure sensor and all the valves.

[0016] The gas storage tank is connected with a CO2 source through a first valve.

[0017] The first valve is connected with the controller.

[0018] The controller is connected with a control touch screen.

[0019] A first air pressure regulating valve and a one-way valve are arranged on the first cabin.

[0020] A second air pressure regulating valve and a one-way valve are arranged on the second cabin.

[0021] A third air pressure regulating valve and a one-way valve are arranged on the gas storage tank.

[0022] A first pressure relief valve is arranged on the first cabin.

[0023] A second pressure relief valve is arranged on the second cabin.

[0024] The implementation manner of the present application is as follows: a full-automatic euthanasia device with a carbon dioxide recycling function comprises a rack, a gas supply pipe, a first cabin, a second cabin, a gas storage tank, an exhaust fan, a pressurized conveying fan, a full-glass door, a control touch screen, a CO2 concentration sensor, a pressure sensor, a controller, an actuator, a sealing valve, a one-way valve, an air pressure regulating valve and a pressure relief valve.

[0025] The first chamber, the second chamber, and the gas storage tank are independent, sealed chambers connected by pipelines and controlled by valves. CO2 gas from the high-pressure cylinder or supply pipe is automatically introduced into the pre-vacuumed gas storage tank under pressure differential for temporary storage. During fully automated system operation, the euthanasia chamber is pre-vacuumed by an exhaust fan. When the negative pressure reaches -300 to -800 Pa within 15–45 seconds, the valves of the gas storage tank and the chambers are opened, allowing CO2 gas to rapidly enter and replace the air in the euthanasia chamber. Excess air in the chamber is discharged through a pressure relief valve, maintaining the chamber pressure. The replacement rate (10%–30% / minute) and total replacement rate (30%–70%) meet the latest animal welfare standards, quickly reaching the CO2 concentration. A CO2 sensor in the euthanasia chamber monitors the CO2 concentration in real time, ensuring that the CO2 concentration remains at a euthanasia level during the euthanasia process. After euthanasia, the CO2 mixture in the chamber is recovered to a storage tank via a pressurized blower, and the pressure inside the chamber is restored through a pressure regulating valve. Simultaneously, an exhaust fan purifies and exhausts the chamber. Under real-time monitoring by a CO2 sensor, the CO2 concentration is ensured to meet the operator's safety standards when the door is opened. The recovered CO2 gas in the storage tank will be replenished with high-concentration CO2 gas according to the set concentration value, serving as the replacement gas for the next euthanasia treatment. Therefore, by recycling CO2 gas, the amount of CO2 used can be accurately controlled, ensuring its circulation within the sealed chamber or pipelines, guaranteeing the safety and effectiveness of the euthanasia process, while also reducing CO2 emissions and ensuring operational procedures and personnel safety.

[0026] Compared with the prior art, the present invention has the following advantages:

[0027] 1. It features CO2 reuse, effectively reducing CO2 emissions and lowering operating costs.

[0028] 2. By adopting a pre-vacuum method, air is quickly purged and CO2 is replaced, which reduces the time of animal anesthesia and asphyxiation, precisely controls the euthanasia time, CO2 concentration and dosage, and effectively protects animal welfare.

[0029] 3. It has a complete CO2 detection system, and all CO2 gas is used in closed pipelines and chambers, effectively preventing CO2 gas leakage or residue from endangering the health of operators.

[0030] 4. The fully sealed glass door not only achieves fully sealed safety management of the chamber, but also allows for real-time observation of the euthanasia status of the experimental animals inside the chamber, and enables intuitive confirmation of the euthanasia results.

[0031] 5. The first and second cabins can be used individually or simultaneously, saving time for euthanasia.

[0032] 6. Pre-set formula, one-button start, fully automatic control of the process, no need for real-time operation and monitoring by operators, improving operator comfort and reducing occupational hazards from exposure to CO2 gas.

[0033] 7. Euthanasia can be performed without a specific carrier, and it has strong compatibility.

[0034] 8. It has audible and visual display and warning functions for operating status, combining warning and status display functions, which better meets the requirements for safe operation.

[0035] 9. Real-time monitoring and control during operation; forced ventilation and early warning in case of interruption to avoid the risk of misoperation and leakage. Attached Figure Description

[0036] Figure 1 This is a schematic diagram of the fully automated euthanasia treatment device with carbon dioxide recycling according to the present invention;

[0037] Figure 2 This is a structural diagram of the fully automated euthanasia treatment device with carbon dioxide recycling according to the present invention;

[0038] Figure 3 This is a front view of the structural design of the fully automated euthanasia treatment device with carbon dioxide recycling according to the present invention;

[0039] Figure 4 This is a side view of the structural design of the fully automated euthanasia treatment device with carbon dioxide recycling according to the present invention;

[0040] Figure 5 This is a 3D front perspective view of the appearance structure of the present invention;

[0041] Figure 6 This is a top view of the external structure of the present invention;

[0042] Figure 7 This is a 3D rear view design drawing of the external structure of the present invention;

[0043] Figure 8 This is a rear view design drawing of the external structure of the present invention;

[0044] Figure 9 This is a side view design drawing of the external structure of the present invention;

[0045] Figure 10 This refers to the pressure change inside the euthanasia chamber during vacuum extraction;

[0046] Figure 11 Changes in CO2 solubility in the euthanasia chamber during CO2 filling;

[0047] Figure 12 Pressure changes inside the euthanasia chamber during CO2 filling;

[0048] Figure 13 This refers to the change in CO2 concentration inside the comfort chamber during self-cleaning.

[0049] in, Figure 2 In the middle, 100 is the electrical control cabinet, 101 is the first compartment, 102 is the second compartment, 103 is the gas storage tank, 104 is the accessories cabinet, and 105 is the frame and casters;

[0050] Figure 3 In the diagram, 200 is an indicator light, 201 is a power switch, 202 is a start button, 203 is an emergency stop button, 204 is a touch screen, 205 is a rotor flow meter, and 206 is a pressure gauge.

[0051] Figure 4 In the diagram, 301 is the exhaust fan, 302 is the pressurized conveying fan, 401 is the CO2 flow meter, 411 is the first CO2 solubility sensor, 412 is the second CO2 solubility sensor, 413 is the third CO2 solubility sensor, 421 is the first pressure sensor, 422 is the second pressure sensor, 423 is the third pressure sensor, 431 is the first door sensor, 432 is the second door sensor, 501 is the first valve, 502 is the second valve, 503 is the third valve, 504 is the fourth valve, 511 is the fifth valve, 512 is the sixth valve, 513 is the seventh valve, 521 is the eighth valve, 522 is the ninth valve, 523 is the tenth valve, 531 is the first pressure relief valve, 532 is the second pressure relief valve, 601 is the first check valve, 602 is the second check valve, 603 is the third check valve, 701 is the exhaust duct, and 702 is the intake duct. Detailed Implementation

[0052] like Figure 1 , Figure 2 , Figure 3 and Figure 4As shown, a fully automated euthanasia treatment device with carbon dioxide recycling includes: a frame and casters 105; a first compartment 101 and a second compartment 102 mounted on the frame; a gas storage tank 103; a control cabinet; and an accessory cabinet (i.e., an electrical control cabinet 100). The first compartment 101 is connected to the gas storage tank 103 via a second valve 502, and the second compartment 102 is connected to the gas storage tank 103 via a third valve 503; a pressurized conveying fan 302, one end of which is connected to the gas storage tank 103 via a fourth valve 504, and the other end of which is connected to the first compartment 101 and the second compartment 102 via second and third valves 502 and 503; an exhaust fan 301, the first compartment 101 is connected to the exhaust fan 301 via a fifth valve 511, the second compartment 102 is connected to the exhaust fan 301 via a sixth valve 512, and the gas storage tank 103 is connected to the exhaust fan 301 via a seventh valve 513. The system includes: a first CO2 solubility sensor 411 and a first pressure sensor 421 located in the first compartment 101; a second CO2 solubility sensor 412 and a second pressure sensor 422 located in the second compartment 102; a third CO2 solubility sensor 413 and a third pressure sensor 423 located in the gas storage tank 103; and a controller connected to the first CO2 solubility sensor 411, the first pressure sensor 421, the second CO2 solubility sensor 412, the second pressure sensor 422, the third CO2 solubility sensor 413, the third pressure sensor 423, and all first to tenth valves. The euthanasia treatment equipment is connected to an external CO2 gas source via the first valve 501. The eighth valve 521 in the first compartment, the ninth valve 522 in the second compartment, and the tenth valve 523 in the gas storage tank 103 are connected to the controller. The eighth valve 521, the ninth valve 522, and the tenth valve 523 are all pressure regulating valves. The eighth valve 521 is connected to the first compartment 101 via a one-way valve (first one-way valve 601), the ninth valve 522 is connected to the second compartment 102 via a one-way valve (second one-way valve 602), and the tenth valve 523 is connected to the gas storage tank 103 via a one-way valve (third one-way valve 603). One end of the first pressure relief valve 531 is connected to the first compartment 101, and one end of the second pressure relief valve 532 is connected to the second compartment 102. The other ends of both the first pressure relief valve 531 and the second pressure relief valve 532 are connected to the exhaust duct 701. The exhaust duct 701 is connected to the exhaust fan 301, and the air inlet duct 702 is connected to the pressurized conveying fan 302. The controller is connected to a control touchscreen. Sensors are installed on the door of the first compartment 101 and the door of the second compartment 102.

[0053] like Figure 2 As shown, the fully automatic euthanasia treatment device with carbon dioxide recycling of the present invention also includes: indicator light 200, power switch 201, start button 202, emergency stop button 203, touch screen 204, rotor flow meter 205, pressure gauge 206, and is set using existing technology.

[0054] like Figures 5 to 9 The image shown is an external view of the fully automated euthanasia treatment device with carbon dioxide recycling according to the present invention. The following examples further illustrate this:

[0055] 1. Main Configuration

[0056] ●Top-mounted electrical control cabinet, 2 euthanasia chambers, 1 gas storage tank, piping and valve system, rack;

[0057] ●Mechanical side-opening fully transparent tempered glass sealed door;

[0058] ●Control and monitor CO2 usage, and install a total flow meter in the supply pipeline;

[0059] ● The main power supply uses an isolated power supply and has a power indicator light;

[0060] ●Each compartment is equipped with a CO2 concentration sensor, a pressure sensor, and a CO2 rotor flow meter.

[0061] ●The gas storage tank is equipped with a CO2 concentration sensor, a pressure sensor, and a pressure gauge;

[0062] ●All compartments and gas tanks are equipped with pressure regulating valves and check valves;

[0063] ●The comfort compartment is equipped with a pressure relief valve;

[0064] ● Three-color operation indicator light status display. Red indicates fault, yellow indicates operation, and blue indicates standby (no CO2 in the cabin);

[0065] ●Exhaust fans and pressurized conveyor fans;

[0066] ● Buzzer alarm;

[0067] ● Sealed with stainless steel sheets on three sides;

[0068] ● Removable silent wheels.

[0069] 2. Main functions

[0070] 1) Touchscreen display, PLC control;

[0071] ① Formulas: Cyclic Euthanasia Formula and Rapid Euthanasia Formula;

[0072] ②CO2 solubility display: CO2 solubility in the two chambers and the gas storage tank;

[0073] ③Pressure display: Pressure of the two compartments and the gas tank;

[0074] ④CO2 Consumption: Total consumption flow rate display, and cabin flow rate display.

[0075] ⑤ Manual control;

[0076] ⑥ Alarms (historical alarms, current alarms);

[0077] ⑦ Parameter settings (system parameters, alarm parameters, time and date).

[0078] 2) Two operating formulas: one-click start of the cyclical euthanasia formula or the rapid euthanasia formula;

[0079] Settings and display via touchscreen, control via touchscreen and buttons.

[0080] 3) Reduce CO2 emissions, recycle CO2 while controlling euthanasia concentration, and adjust euthanasia time (0~

[0081] (600 seconds), which meets animal welfare requirements;

[0082] 4) The exhaust pre-vacuum and CO2 flow rate and replacement time are adjustable (5-600 seconds), which is fast and efficient;

[0083] 5) Each chamber can undergo euthanasia treatment for 6 to 8 IVC cage bottoms simultaneously;

[0084] 6) It has a real-time operating status display function;

[0085] 7) Forced purification and exhaust function;

[0086] If there is a high concentration of carbon dioxide in the cabin, the system will open the exhaust fan and pressure regulating valve and force ventilation for 200-600 seconds when the door is forcibly opened or an emergency stop is triggered.

[0087] 8) Forced power-off protection function;

[0088] In the event of a forced power outage, all valves will automatically lock. After power is restored, the system will perform a self-check, and if it detects CO2 concentration in the cabin, it will automatically activate the forced ventilation formula.

[0089] 3. Cyclic Comfort Formula

[0090] 1) Open the hatch to load the IVC cage bottom, then close the hatch;

[0091] 2) Open the manual valve for CO2 supply;

[0092] 3) Power on the equipment and start it up;

[0093] 4) Choose a euthanasia formula;

[0094] 5) Start the recipe; the system will run automatically.

[0095] ① Check if the door is closed; proceed to the next step if it is closed, otherwise issue a warning and do not run.

[0096] ② Detect the pressure and CO2 concentration inside the gas storage tank 103. If the pressure or CO2 concentration is insufficient, open the first valve 501 and the fourth valve 504 until the pressure and CO2 concentration inside the gas storage tank 103 reach the set values.

[0097] ③ Start the exhaust fan 301, open the fifth valve 511 or the sixth valve 512. After the pre-vacuum is completed, the valves and exhaust fans of the corresponding compartments will be automatically closed.

[0098] ④ Open the fourth valve 504 connected to the gas storage tank 103 and the second valve 502 connected to the first compartment 101 or the third valve 503 connected to the second compartment 102. CO2 gas will automatically and rapidly diffuse to the first compartment 101 or the second compartment 102 under the action of pressure difference. The air in the compartment will be discharged through the pressure relief valve 531 or the pressure relief valve 532. At the same time, the Anle formula timing will start automatically.

[0099] ⑤ When the CO2 concentration in the comfort chamber reaches the set value, close the fourth valve 504, the second valve 502, or the third valve 503. During operation, if the CO2 concentration in the chamber is lower than the set value, step ④ will be automatically repeated.

[0100] ⑥ After the euthanasia formula timing is completed, turn on the CO2 pressurizing fan 302, and simultaneously turn on the second valve 502 connected to the first compartment 101 or the third valve 503 connected to the second compartment 102 and the fourth valve 504 connected to the gas storage tank 103 to pump the CO2 mixture into the gas storage tank 103. When the negative pressure in the euthanasia chamber reaches the set value, close all valves and the pressurizing fan;

[0101] ⑦ Turn on the exhaust fan 301 in the comfort chamber, open the fifth valve 511 or the sixth valve 512, and at the same time open the eighth valve 521 connected to the first chamber 101 or the ninth valve 522 connected to the second chamber 102. The system automatically detects the CO2 concentration. When the CO2 concentration reaches the safety set value, turn off the exhaust fan 301 and close the fifth valve 511 or the sixth valve 512. After 5 seconds, close the eighth valve 521 or the ninth valve 522.

[0102] ⑧ When the formula operation is complete, the buzzer will sound as an alarm, and the status indicator light will flash blue.

[0103] 6) Manually open the door and remove the IVC cage.

[0104] 4. Fast-acting euthanasia formula

[0105] 1) Open the hatch to load the IVC cage bottom, then close the hatch;

[0106] 2) Open the manual valve for carbon dioxide supply;

[0107] 3) Power on the equipment and start it up;

[0108] 4) Choose a fast-acting euthanasia formula;

[0109] 5) Start the recipe; the system will run automatically.

[0110] ① Check if the door is closed; proceed to the next step if it is closed, otherwise issue a warning and do not run.

[0111] ② Start the exhaust fan 301, open the fifth valve 511 or the sixth valve 512, and after the pre-vacuum is completed, automatically close the valve and exhaust fan of the corresponding compartment;

[0112] ③ Open the first valve 501, the second valve 502 connected to the first compartment 101, or the third valve 503 connected to the second compartment 102. CO2 gas will automatically and rapidly diffuse to the first compartment 101 or the second compartment 102 under the action of pressure difference. The air in the compartment will be discharged through the pressure relief valve 531 or the pressure relief valve 532. At the same time, the euthanasia formula timing will start automatically.

[0113] ④ When the CO2 concentration in the euthanasia chamber reaches the set value, close the first valve 501, the second valve 502, or the third valve 503; during operation, if the CO2 concentration in the euthanasia chamber is lower than the set value, step ③ will be automatically repeated.

[0114] ⑤ After the euthanasia formula timing ends, turn on the euthanasia chamber exhaust fan 301, open the fifth valve 511 or the sixth valve 512, and at the same time open the eighth valve 521 connected to the first chamber 101 or the ninth valve 522 connected to the second chamber 102. The system automatically detects the CO2 concentration. When the CO2 concentration reaches the safety set value, turn off the exhaust fan 301, and at the same time close the fifth valve 511 or the sixth valve 512. After 5 seconds, close the eighth valve 521 or the ninth valve 522.

[0115] ⑥ When the formula operation is complete, the buzzer will sound as an alarm, and the status indicator light will flash blue.

[0116] 6) Manually open the door and remove the IVC cage.

[0117] 5. Purified and safe formula

[0118] 1) When the system alarms or the door is forcibly opened, the purification safety formula is triggered. The yellow and red lights flash simultaneously.

[0119] 2) The system starts the exhaust fan 301, opens the fifth valve 511 or the sixth valve 512, and simultaneously opens the eighth valve 521 connected to the first compartment 101 or the ninth valve 522 connected to the second compartment 102. The system automatically detects the CO2 concentration. When the CO2 concentration reaches the safety set value, the exhaust fan 301 is turned off, and the fifth valve 511 or the sixth valve 512 is closed. After 5 seconds, the eighth valve 521 and the ninth valve 522 are closed.

[0120] 3) When the formula operation is complete, the buzzer will sound as a warning and the status indicator light will flash blue.

[0121] 6. Other

[0122] 1) When using the cyclic euthanasia formula, the first chamber 101 and the second chamber 102 can be used independently. After CO2 replenishment is completed in one chamber, the other chamber can be opened in a cross-continuous manner.

[0123] 2) External dimensions: 905 mm long, 480 mm wide, and 1920 mm high;

[0124] 3) The euthanasia cabin is approximately 130 liters high and has two levels;

[0125] 4) Gas storage tank 103, approximately 75 liters, made of 304 stainless steel, designed pressure ≤ 4 bar;

[0126] 5) Cage height ≤ 190 mm;

[0127] 6) Rapid pre-vacuuming and rapid carbon dioxide gas introduction reduce animal asphyxiation time and increase animal welfare;

[0128] 7) such as Figure 10 As shown, the pressure change inside the euthanasia chamber during vacuuming is illustrated. A differential pressure gauge is pre-connected to the euthanasia chamber via a hose to measure the pressure difference between the chamber and the operating room in real time. During the pre-vacuum stage of the euthanasia formula, the chamber pressure changes as the exhaust fan operates. The negative pressure value inside the chamber during the pre-vacuum stage is recorded by the differential pressure gauge. During the pre-vacuum process, the chamber pressure eventually reaches approximately -730 Pa and remains at -730 Pa. Therefore, the chamber's design stiffness requirements can be determined to ensure that the chamber walls do not deform or leak during pre-vacuuming.

[0129] 8) such as Figure 11 As shown, the CO2 solubility change in the euthanasia chamber during CO2 filling was observed. A CO2 solubility detector was placed in the euthanasia chamber beforehand, and then the euthanasia formula was run. After pre-vacuuming, the CO2 solubility value in the chamber was recorded. By observing the changes in CO2 solubility, and considering the required CO2 replacement rate of 30%–70% and the required ventilation rate (10%–30% / minute) for animal euthanasia, the relationship between CO2 solubility and filling time was determined. A 186-liter euthanasia chamber was filled with 2000 liters / hour of pure CO2. After 2.5 minutes of filling, the CO2 solubility reached 31.63%; after 5 minutes, it reached 53.92%; and after 8 minutes, it reached 70.35%. These tests indicate that the CO2 replacement rate meets the national standard requirements.

[0130] 9) such as Figure 12As shown, the pressure change inside the euthanasia chamber during CO2 filling is illustrated. A differential pressure gauge is pre-connected to the euthanasia chamber via a hose to measure the pressure difference between the chamber and the operating room in real time. After the euthanasia formula is activated, the chamber pressure reaches a certain negative pressure value as the exhaust fan operates. After the exhaust fan is turned off and the CO2 inlet valve is opened, the chamber pressure gradually rises from -730 Pa and eventually stabilizes at around 23 Pa (the euthanasia chamber is equipped with a pressure relief valve to remove excess air and prevent excessive pressure inside the chamber).

[0131] 10) such as Figure 13 As shown, the CO2 solubility change in the euthanasia chamber during self-cleaning is illustrated. A detector capable of measuring high and low CO2 solubility is placed in the euthanasia chamber beforehand. The euthanasia formula is then run. When the CO2 solubility in the chamber reaches 70%, the CO2 inlet valve is closed and kept sealed for 60 seconds. Then, the exhaust fan is started, the pressure regulating valve is opened, and the readings of the high and low CO2 solubility detectors are recorded. If the low solubility detector shows no data, the real-time CO2 solubility value of the high solubility detector is recorded; otherwise, the real-time CO2 solubility value of the low solubility detector is recorded. Testing shows that it takes approximately 9.5 minutes for the CO2 solubility in the euthanasia chamber to decrease from 71.53% to a safe level (0.1% or 1000 ppm).

[0132] Testing showed that during the pre-vacuum phase of the euthanasia formula, the pressure in both the first and second euthanasia chambers reached -731 Pa. After the pre-vacuum phase, CO2 gas was introduced. At a ventilation rate of 2000 L / h, the CO2 concentration inside the chambers rose to 35.81% within 3 minutes and reached 53.14% within 5 minutes, achieving a high-concentration environment for rapid anesthesia and euthanasia of animals. During the euthanasia process, the pressure inside the chambers remained below 23 Pa. In the self-cleaning formula, the CO2 concentration inside the euthanasia chambers reached a safe level of less than 1000 ppm within 10 minutes.

[0133] Test parameters and tools:

[0134] ◆ Comfort cabin volume: Approximately 186 liters (Length 1050 * Width 660 * Height 270 mm)

[0135] ◆Pressure measuring instrument: Detection range 0~10000Pa, accuracy 0.1Pa

[0136] ◆CO2 low solubility detector: Detection range 0~10000ppm, accuracy 1ppm

[0137] ◆CO2 high solubility detector: Detection range 0%~100%, accuracy 0.01%

[0138] ◆ Measuring tape; 0-3 meters, accuracy 1 millimeter

[0139] ◆Timer; 0–30 minutes, accuracy 1 second

[0140] ◆Exhaust fan: 2-inch, 12V fan, air volume 66 cubic meters / hour, air pressure 570Pa

[0141] Note: The methods and data in this test are for prototype design analysis, instrument selection reference, and formula parameter design only.

Claims

1. A fully automated euthanasia treatment device with carbon dioxide recycling, characterized in that, include: frame; The first and second compartments are located on the rack; The gas storage tank is connected to the first compartment via a second valve and the second compartment via a third valve. A pressurized conveying fan, one end of which is connected to the gas storage tank via a fourth valve, and the other end of which is connected to the first compartment and the second compartment via a second valve and a third valve; The exhaust fan is connected to the first compartment via a fifth valve, the second compartment via a sixth valve, and the gas storage tank via a seventh valve. A first CO2 concentration sensor and a first pressure sensor are installed inside the first chamber; A second CO2 solubility sensor and a second pressure sensor are installed inside the second chamber; A third CO2 concentration sensor and a third pressure sensor are installed inside the gas storage tank; The controllers are all connected to the first CO2 solubility sensor, the first pressure sensor, the second CO2 solubility sensor, the second pressure sensor, the third CO2 solubility sensor, the third pressure sensor, and all valves.

2. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, The gas storage tank is connected to a CO2 gas source via a first valve.

3. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 2, characterized in that, The first valve is connected to the controller.

4. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, The controller is connected to a control touchscreen.

5. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, The first compartment is equipped with a first air pressure regulating valve and a one-way valve.

6. The fully automated euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, The second compartment is equipped with a second air pressure regulating valve and a one-way valve.

7. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, The gas storage tank is equipped with a third pressure regulating valve and a one-way valve.

8. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, A first pressure relief valve is installed on the first compartment.

9. The fully automatic euthanasia treatment equipment with carbon dioxide recycling as described in claim 1, characterized in that, A second pressure relief valve is installed on the second compartment.

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