Lactation promoting traditional Chinese medicine for promoting lactation of lactating sows as well as preparation method and application of lactation promoting traditional Chinese medicine

Abstract

The invention discloses a lactation promoting traditional Chinese medicine for promoting lactation of lactating sows and a preparation method and application thereof.The lactation promoting traditional Chinese medicine is prepared from cowherb seeds, uniflower swisscentaury roots, liquidambar formosana hance, verbena officinalis, angelica sinensis, leonurus japonicus, astragalus membranaceus and liquorice according to a specific proportion, and the raw materials are subjected to water extraction and concentration and then mixed with cyclodextrin to be prepared into granules. The lactagogue traditional Chinese medicine can significantly improve the lactation yield of lactating sows and the maternal antibody level in milk, especially the IgG and IgM content, and can improve the plasma immune globulin and prolactin level, thereby effectively promoting the growth of piglets and reducing the diarrhea rate.

Description

TECHNICAL FIELD

[0001] The present application relates to a lactation-promoting traditional Chinese medicine for lactating sows and a preparation method and application thereof, and belongs to the technical field of animal nutrition and feed additives. BACKGROUND

[0002] After farrowing, the growth and development of piglets are highly dependent on the lactation performance and maternal antibody level of sows, and the lactation performance and mucosal immune function of sows directly determine the growth and development of piglets. At present, measures to promote the lactation performance of sows include nutritional regulation and drug regulation, which can promote lactation to some extent, but cannot effectively promote the mucosal immune function of sow milk and improve the immune function of lactating piglets. At present, the measures for promoting lactation of sows in the breeding industry mainly include nutritional regulation and drug regulation. Nutritional regulation mainly adjusts the energy, protein level and amino acid balance in the diet, which can increase milk yield to some extent, but this method often focuses on conventional indicators such as milk protein and milk fat, and lacks effective improvement of the mucosal immune function of milk (i.e. maternal antibody level). Therefore, it is impossible to fundamentally enhance the immune ability of piglets. In terms of drug regulation, in addition to the use of chemical drugs, traditional Chinese medicine has attracted attention due to its naturalness and low residue. There are many classical prescriptions (such as Tongluo Powder) with the effect of promoting lactation in traditional Chinese medicine theory, which are often used to solve the problem of postpartum lack of lactation in lactating sows. However, these traditional Chinese medicine prescriptions mainly focus on “lactation promotion”, and their original intention and application effect are mainly concentrated on promoting mammary gland development and milk secretion, and lack of development and application for the specific function of “improving milk immune activity”. Therefore, in view of the defects and deficiencies in the prior art, it is of great significance to obtain a lactation-promoting traditional Chinese medicine for lactating sows, which is convenient to use, has the functions of lactation promotion and milk immune function improvement, promotes lactation of lactating sows, improves milk mucosal immune function, enhances the immunity of piglets and promotes the growth and development of piglets, and improves the overall efficiency of pig breeding industry. SUMMARY

[0003] The present application aims to provide a lactation-promoting traditional Chinese medicine for lactating sows and a preparation method and application thereof. The present application can effectively promote lactation of sows, significantly improve the maternal antibody level of sow milk, enhance the immunity of piglets, promote the growth and development of piglets, and improve the overall efficiency of pig breeding industry.

[0004] To solve the above technical problems, the technical solution provided by the present application is as follows: a lactation-promoting traditional Chinese medicine for lactating sows, which comprises the following raw materials by weight: Herba Epimedii 3-8 parts, Rhizoma Rhizomatis 3-8 parts, Herba Lamiophlomis 2-6 parts, Herba Verbena 2-6 parts, Radix Angelicae Sinensis 2-6 parts, Herba Leonuri 2-6 parts, Radix Astragali 2-6 parts, and Radix Glycyrrhizae 1-5 parts.

[0005] The lactation-promoting traditional Chinese medicine for lactating sows, by weight, comprises the following raw materials: Herba Galgeae 4-6 parts, Radix Rhapontici 4-6 parts, Herba Rubi 3-4 parts, Herba Valerianae 3-4 parts, Herba Leonuri 3-4 parts, Radix Astragali 3-4 parts, and Radix Glycyrrhizae 2-3 parts.

[0006] The lactation-promoting traditional Chinese medicine for lactating sows, by weight, comprises the following raw materials: Herba Galgeae 4-6 parts, Radix Rhapontici 4-6 parts, Herba Rubi 3-4 parts, Herba Valerianae 3-4 parts, Herba Leonuri 3-4 parts, Radix Astragali 3-4 parts, and Radix Glycyrrhizae 2-3 parts.

[0007] A preparation method of the lactation-promoting traditional Chinese medicine, comprising the following steps: Step 1, water extraction and decoction of the raw medicinal materials twice, and combining the extraction liquid; Step 2, vacuum concentration of the extraction liquid to obtain dry extract; Step 3, addition of cyclodextrin to the dry extract, mixing uniformly, and preparation of granules.

[0008] The preparation method, the weight ratio of the dry extract to cyclodextrin is 1:2.5.

[0009] An application of the lactation-promoting traditional Chinese medicine in preparing a feed additive for increasing the lactation amount of lactating sows.

[0010] An application of the lactation-promoting traditional Chinese medicine in preparing a feed additive for increasing the level of maternal antibodies in the milk of lactating sows.

[0011] The application, the maternal antibodies include one or more of IgA, IgG, and IgM.

[0012] An application of the lactation-promoting traditional Chinese medicine in preparing a feed additive for reducing the diarrhea rate of piglets.

[0013] An application of the lactation-promoting traditional Chinese medicine in preparing a feed additive for increasing the content of immunoglobulin and prolactin in the plasma of lactating sows.

[0014] Compared with the prior art, the present application has the following remarkable beneficial effects: 1. The traditional Chinese medicine can effectively increase the lactation amount of lactating sows, thereby providing more sufficient nutrition source for piglets. Tests show that the traditional Chinese medicine can significantly promote the average daily weight gain of piglets, and effectively reduce the diarrhea rate of piglets. This proves that the present application not only solves the problem of insufficient lactation, but also promotes the healthy growth of piglets by improving the quality of milk.

[0015] 2, The invention breaks through the limitation of traditional lactation promoting prescription which only focuses on milk yield, and can effectively improve the content of key immunoglobulin in milk, including IgG and IgM. This means that piglets sucking breast milk can obtain higher levels of maternal antibodies, and their passive immunity and disease resistance are fundamentally enhanced.

[0016] 3, The beneficial effects of the invention not only manifest in milk, but also act on the sow itself. The traditional Chinese medicine can effectively improve the content of immunoglobulin (such as IgA, IgM) and prolactin (PRL) in sow plasma, which indicates that the invention can systematically regulate the immune function and lactation endocrine of lactating sows, and mechanismally guarantee the continuous improvement of lactation performance and milk quality.

[0017] 4, All components of the invention are traditional Chinese medicines, which have no toxic side effects and no drug residue concerns, avoid the safety hazards and drug resistance problems that may be brought by chemical drugs, and meet the green and healthy development needs of modern animal husbandry. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 is the content comparison of IgA in colostrum; Figure 2 is the content comparison of IgG in milk on the 10th day; Figure 3 is the content comparison of IgM in milk on the 21st day; Figure 4 is the content comparison of IgA in plasma; Figure 5 is the content comparison of IgG in plasma; Figure 6 is the content comparison of IgM in plasma; Figure 7 is the content comparison of prolactin (PRL) in plasma. DETAILED DESCRIPTION

[0019] The invention will be further described below in conjunction with examples.

[0020] Example 1: Lactation promoting traditional Chinese medicine for lactating sows and its preparation, including raw material weighing: weighing 6 kg of cowslip, 6 kg of radix eupatorium, 3 kg of radix rubiae, 3 kg of verbena, 3 kg of angelica, 3 kg of motherwort, 3 kg of astragalus, and 3 kg of licorice; Mix the above medicinal materials, add 10 times the amount of water and decoct for 2 hours, filter out the medicinal liquid; add 8 times the amount of water to the residue again, decoct for 1.5 hours, and filter out the medicinal liquid. Combine the two obtained medicinal liquids and pass through a 200 mesh sieve.

[0021] Vacuum concentrate the filtered medicinal liquid under the condition of 65℃ and -0.08 MPa, to obtain a thick paste with a relative density of 1.25 (measured at 60℃), and continue to concentrate to dry extract.

[0022] To the dry extract, 2.5 times its weight of cyclodextrin is added, mixed evenly, and granulated using a wet granulator, dried at 60°C, and packaged, to obtain the lactation-promoting traditional Chinese medicine granules of the present application.

[0023] Example 2: Lactation-promoting traditional Chinese medicine for promoting lactation in sows and its preparation, including the following steps: 5 kg of Herba semen astragali, 5 kg of Radix seu Rhizoma Polygoni Avicularis, 3.8 kg of Herba Leonuri, 3.8 kg of Herba Leonuri, 3.8 kg of Radix Angelicae Sinensis, 3.8 kg of Herba Leonuri, 3.8 kg of Radix Paeoniae Alba, and 2.5 kg of Radix Glycyrrhizae are weighed.

[0024] The above medicinal materials are mixed, 10 times the amount of water is added and decocted for 2 hours, and the medicinal liquid is filtered out; the residue is added with 8 times the amount of water and decocted for 1.5 hours, and the medicinal liquid is filtered out. The medicinal liquids obtained in the two steps are combined and sieved through a 200-mesh sieve.

[0025] The filtered medicinal liquid is vacuum concentrated at 65°C and -0.08 MPa to obtain a thick paste with a relative density of 1.25 (measured at 60°C), and further concentrated to dry extract.

[0026] To the dry extract, 2.5 times its weight of cyclodextrin is added, mixed evenly, and granulated using a wet granulator, dried at 60°C, and packaged, to obtain the lactation-promoting traditional Chinese medicine granules of the present application.

[0027] Example 3: Lactation-promoting traditional Chinese medicine for promoting lactation in sows and its preparation, including the following steps: 8 kg of Herba semen astragali, 4 kg of Radix seu Rhizoma Polygoni Avicularis, 4 kg of Herba Leonuri, 4 kg of Herba Leonuri, 4 kg of Radix Angelicae Sinensis, 3.5 kg of Herba Leonuri, 3.5 kg of Radix Paeoniae Alba, and 2 kg of Radix Glycyrrhizae are weighed.

[0028] The above medicinal materials are mixed, 10 times the amount of water is added and decocted for 2 hours, and the medicinal liquid is filtered out; the residue is added with 8 times the amount of water and decocted for 1.5 hours, and the medicinal liquid is filtered out. The medicinal liquids obtained in the two steps are combined and sieved through a 200-mesh sieve.

[0029] The filtered medicinal liquid is vacuum concentrated at 65°C and -0.08 MPa to obtain a thick paste with a relative density of 1.25 (measured at 60°C), and further concentrated to dry extract.

[0030] To the dry extract, 2.5 times its weight of cyclodextrin is added, mixed evenly, and granulated using a wet granulator, dried at 60°C, and packaged, to obtain the lactation-promoting traditional Chinese medicine granules of the present application.

[0031] Example 4: Application test of lactation-promoting traditional Chinese medicine granules in improving lactation and maternal antibody level in sows.

[0032] 1. Test design: 16 healthy and long-growing pregnant sows with similar parity, body condition and expected date of delivery were selected and randomly divided into 2 groups, 8 sows in each group. The control group was fed with basal diet, and the test group was added with 2.0% lactation-promoting Chinese medicine granules prepared by the method of Example 2 in the basal diet. The test started from the first day after the sows delivered and lasted for 21 days. All sows were allowed to drink water freely, and were fed twice a day, 3 kg each time. The health conditions of sows and piglets were observed and recorded every day.

[0033] 2. Determination index and method: Growth performance: The body weight of piglets was measured at the beginning of the test (within 24 hours after birth) and in the morning of the 21st day, and the average daily gain was calculated; the diarrhea of piglets was recorded every day, and the diarrhea rate was calculated.

[0034] Lactation yield determination: The lactation yield of sows was determined by weighing and sucking method on the 21st day of lactation.

[0035] Milk immune index: Milk samples were collected on the 1st day (colostrum), 10th day and 21st day after delivery, and the contents of IgA, IgG and IgM in milk were determined by enzyme-linked immunosorbent assay (ELISA).

[0036] Plasma index: The plasma of sows was collected on the 21st day of the test, and the contents of IgA, IgG, IgM and prolactin (PRL) in plasma were determined by ELISA.

[0037] 3. Test results: 3.1 Effect on the growth performance of nursing piglets, the test results are shown in Table 1: Item Control group Test group Value Initial birth weight of piglets 2.25±0.91 2.14±0.64 0.137 Average daily weight gain of piglets 151.40±2.54 218.05±2.88 0.000 Diarrhea rate of piglets 9.23±0.70 4.81±1.31 0.010 Lactation of sows 38.40±3.12 52.09±1.79 0.019 Table 1 Compared with the control group, the average daily gain of piglets in the test group was significantly increased (P<0.01), the diarrhea rate of piglets was significantly reduced (P<0.05), and the lactation yield of sows was significantly increased (P<0.05). There was no significant difference in the birth weight of piglets between the two groups (P>0.05).

[0038] 3.2 Effect on the milk immune index of nursing sows. The results are shown in Figures 1-3 , Figure 1 is the comparison of IgA content in colostrum, Figure 2 is the comparison of IgG content in milk on the 10th day, Figure 3 is the comparison of IgM content in milk on the 21st day. It can be seen that the immune globulin content in milk of the test group was significantly higher than that of the control group. Among them, the IgG content in milk on the 10th day of lactation was significantly higher than that of the control group (P<0.05), and the IgM content in milk on the 21st day of lactation was extremely significantly higher than that of the control group (P<0.01). There was no significant difference in IgA content in colostrum between the two groups.

[0039] 3.3 The influence of immune indexes and prolactin in blood plasma of lactating sows. The results are shown as follows, Figures 4-7 Figure 4 is the content of IgA in blood plasma, Figure 5 is the content of IgG in blood plasma, Figure 6 is the content of IgM in blood plasma, Figure 7 is the content of prolactin (PRL) in blood plasma. It can be seen that, compared with the control group, the content of IgM in blood plasma of sows in the test group is significantly increased (P<0.05), and the contents of IgA and prolactin (PRL) are extremely significantly increased (P<0.01). Although the content of IgG has an increasing trend, the difference is not significant.

[0040] The results of the example show that, by adding 2% of the lactation-promoting Chinese medicine granules in the daily ration, the lactation performance of sows can be significantly improved, the key immunoglobulin (IgA, IgG, IgM) and prolactin levels in milk and blood plasma of sows are increased, thereby effectively promoting the growth of piglets and reducing the diarrhea rate of piglets, and the comprehensive application effect is remarkable.

[0041] The present application can effectively promote lactation of sows and significantly increase the level of milk antibodies of sows, can enhance the immunity of piglets and promote the growth and development of piglets, and improve the overall benefits of pig breeding industry.​

Claims

1. A traditional Chinese medicine for promoting lactation in lactating sows, characterized in that: The ingredients are included by weight as follows: Vaccaria segetalis 3-8 parts, Leucas chinensis 3-8 parts, Luffa cylindrica 2-6 parts, Verbena officinalis 2-6 parts, Angelica sinensis 2-6 parts, Leonurus japonicus 2-6 parts, Astragalus membranaceus 2-6 parts, Glycyrrhiza uralensis 1-5 parts.

2. The galactagogue traditional Chinese medicine for promoting lactation in lactating sows according to claim 1, characterized in that: The ingredients are as follows by weight: 4-6 parts of Vaccaria segetalis, 4-6 parts of Leucas cephalotaxus fortunei, 3-4 parts of Luffa cylindrica, 3-4 parts of Verbena officinalis, 3-4 parts of Angelica sinensis, 3-4 parts of Leonurus japonicus, 3-4 parts of Astragalus membranaceus, and 2-3 parts of Glycyrrhiza uralensis.

3. The galactagogue traditional Chinese medicine for promoting lactation in lactating sows according to claim 2, characterized in that: The ingredients are as follows by weight: 5 parts Wangbuliuxing, 5 parts Loulu, 3.8 parts Lulutong, 3.8 parts Mabiancao, 3.8 parts Danggui, 3.8 parts Yimucao, 3.8 parts Huangqi, and 2.5 parts Gancao.

4. A method for preparing a galactagogue traditional Chinese medicine as described in any one of claims 1-3, characterized in that, Includes the following steps: Step 1: Perform water extraction and decoction twice on the raw materials, and combine the extracts; Step 2: Concentrate the extract under vacuum to obtain a dry extract; Step 3: Add cyclodextrin to the dry extract, mix well, and prepare granules.

5. The preparation method according to claim 4, characterized in that: The weight ratio of the dry extract to cyclodextrin is 1:2.

5.

6. The use of a galactagogue traditional Chinese medicine as described in any one of claims 1-3 in the preparation of a feed additive for increasing milk production in lactating sows.

7. The use of a galactagogue traditional Chinese medicine as described in any one of claims 1-3 in the preparation of a feed additive for increasing the level of maternal antibodies in the milk of lactating sows.

8. The application according to claim 7, characterized in that, The maternal antibodies include one or more of IgA, IgG, and IgM.

9. The use of a galactagogue traditional Chinese medicine as described in any one of claims 1-3 in the preparation of a feed additive for reducing the diarrhea rate of suckling piglets.

10. The use of a galactagogue traditional Chinese medicine as described in any one of claims 1-3 in the preparation of a feed additive for increasing the content of immunoglobulins and prolactin in the plasma of lactating sows.

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