Method for controlling invasion of solanum rostratum

Abstract

The invention provides a method for controlling invasion of solanum rostratum, and relates to the technical field of invasive organism control, the method for controlling invasion of solanum rostratum comprises the following steps: planting alternative plant seeds in soil containing solanum rostratum seeds, and synchronously applying a soil conditioner to the soil; the soil conditioner is selected from at least one of a liquid fungicide containing bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens, a solid bacterial fertilizer containing the bacillus amyloliquefaciens, an organic fertilizer containing mushroom dregs and rice hull powder and an inorganic fertilizer containing nitrogen, P2O5, K2O, boron and zinc. According to the method, rhizosphere micro-ecology and nutrient circulation are effectively improved through soil-soil conditioner coordinated regulation, soil nutrients invading an ecological system are increased, the defect that the effect of a single measure is insufficient is overcome, continuous inhibition on the invasive plant solanum rostratum is achieved, and the method has the advantages of being environmentally friendly and high in operability and has ecological benefits and popularization potential.

Description

Technical Field

[0001] This invention relates to the field of invasive species control technology, and in particular to a method for controlling the invasion of Solanum nigrum. Background Technology

[0002] Solanum nigrum (spiny calyx) Solanum rostratum *Dunal.* is a highly invasive alien plant. *Solanum nigrum* can grow in habitats such as riverbanks, grasslands, wastelands, abandoned farmland, and overgrazed pastures. It has low requirements for soil nutrients and can adapt to variable climates and heterogeneous habitats. This invasive plant reduces crop yields and biodiversity, seriously threatening the local ecological environment, agricultural production, grassland animal husbandry, biodiversity, and human health.

[0003] Control of invasive alien plants such as *Solanum nigrum* relies primarily on two traditional methods: manual removal and chemical herbicide eradication. Manual control requires hiring professional weeding teams, paying them 200 yuan per person per day, with each person only able to complete 1-2 mu (approximately 0.16 acres) per day. This extensive approach has three major drawbacks: first, low efficiency; even with a team of tens of thousands of people, it would take years to complete a comprehensive control effort when dealing with invasive areas often spanning thousands of mu; second, poor precision; due to a lack of real-time monitoring technology, missed areas or over-removal often occur, with some areas experiencing a duplication rate exceeding 40%; and third, high labor intensity, with the critical control period being during the summer months, where the heatstroke rate is as high as 15%, leading to a continuous decline in farmers' willingness to participate. Chemical control is even more extensive, generally employing indiscriminate spraying, resulting in a pesticide utilization rate of less than 30%, and an average annual chemical herbicide usage of 2-3 kg per mu (approximately 0.16 acres), far exceeding international safety standards. This model has created a double negative effect: on the one hand, it leads to high control costs, with direct chemical control costs reaching 80-120 yuan per mu, and the comprehensive cost exceeding 200 yuan after adding soil remediation costs; on the other hand, it causes serious environmental pollution. Field surveys show that in areas where chemical agents are continuously used, the number of beneficial organisms such as earthworms has decreased by 70%, and soil compaction has intensified. More seriously, both traditional methods have the problem of short-lived control effects. The regeneration rate of manually removed plants exceeds 60%, and the effective period of chemical control only lasts for 20-30 days, forcing farmers and herdsmen into a dilemma of repeated investment. This inefficient and high-cost control model ultimately resulted in the overall control rate in the project area hovering below 30% for a long time. In some severely affected areas, the incidence of invasive plants even showed a year-on-year increasing trend, forming a vicious cycle of "the more you control, the more there are," seriously affecting local food security and ecological security.

[0004] Solanum rostratum has strong ecological adaptability and strong reproductive capacity, and traditional control methods cannot effectively control its harm for a long term. The plant replacement control technology is an ecological control method, which selects indigenous plants with economic value or ecological value to control the expansion and spread of invasive plants through interspecific competition effect and community succession law, so as to inhibit the invasive plants. However, only through the plant replacement control technology to control the invasive plants, the system regulation of multiple interactions is lacked, and the sustained control effect of the invasive species needs to be strengthened. SUMMARY

[0005] The application provides a method for controlling the invasion of Solanum rostratum, which solves the defects that the prior art can hardly achieve the sustained control of the invasive species only through the plant replacement control technology, and provides a method for realizing the sustained control of the invasive Solanum rostratum through the system regulation of multiple interactions.

[0006] The application provides a method for controlling the invasion of Solanum rostratum, which solves the defects that the prior art can hardly achieve the sustained control of the invasive species only through the plant replacement control technology, and provides a method for realizing the sustained control of the invasive Solanum rostratum through the system regulation of multiple interactions. The application provides a method for controlling the invasion of Solanum rostratum, which solves the defects that the prior art can hardly achieve the sustained control of the invasive species only through the plant replacement control technology, and provides a method for realizing the sustained control of the invasive Solanum rostratum through the system regulation of multiple interactions. The application provides a method for controlling the invasion of Solanum rostratum, which solves the defects that the prior art can hardly achieve the sustained control of the invasive species only through the plant replacement control technology, and provides a method for realizing the sustained control of the invasive Solanum rostratum through the system regulation of multiple interactions. The application provides a method for controlling the invasion of Solanum rostratum, which solves the defects that the prior art can hardly achieve the sustained control of the invasive species only through the plant replacement control technology, and provides a method for realizing the sustained control of the invasive Solanum rostratum through the system regulation of multiple interactions.

[0007] Soil microorganisms play an important role in ecological processes such as nitrogen cycle in the ecosystem. Alien invasive plants can change the microbial community structure of the invaded area and change the function of the original ecosystem. After the invasion of alien plants, the microbial community structure and soil nutrient characteristics of the invaded area are changed, the mutualistic symbiotic relationship between local plants and microorganisms is destroyed, and the function of the original ecosystem of the invaded area is changed, so as to establish a soil environment conducive to the survival of the alien plants. The change of the local microbial community structure by the alien plants is an important factor for the invasion of the alien plants. After the invasion of Solanum rostratum, the Simpson index and Chao1 index of soil microorganisms are higher than those of local vegetation, but do not reach a significant level, and after the control of the replacement plants, the Shannon index and Chao1 index are significantly reduced (P<0.05). Solanum rostratum significantly reduces the relative abundance of Microbacterium ( Microvirga ), Skermanella ( Skermanella ), Sphingomonas ( Sphingomonas ) and Acidobacteria ( Bryobacter ) in Proteobacteria (P<0.05), and after the restoration of the vegetation, the abundance of these bacteria is also increased. The RDA analysis result shows that soil organic matter, total nitrogen, total phosphorus, total potassium and available potassium are important factors affecting the composition of bacterial community.

[0008] The method for controlling the invasion of Solanum rostratum provided by the application plants alternative plant seeds in soil containing Solanum rostratum seeds and synchronously applies soil conditioners to the soil, and effectively improves the rhizosphere micro-ecology and nutrient circulation through the synergistic regulation of the soil-soil conditioner, thereby increasing the soil nutrients of the ecological system. On the one hand, the method can improve the absorption and utilization effect of the alternative plant on the rhizosphere soil nutrients and the stress resistance of the alternative plant, significantly enhances the planting and competition ability of the alternative plant, and further significantly improves the stability and adaptability of the alternative control. On the other hand, the method can effectively inhibit the seed setting amount (the average number of spiny bracts per Solanum rostratum plant) of Solanum rostratum and reduce the original Solanum rostratum seeds existing in the soil, and the two synergistically reduce the reproduction of Solanum rostratum from the root source. The method for controlling the invasion of Solanum rostratum provided by the application overcomes the defects of insufficient effect of a single measure, realizes the sustained inhibition of the invasive plant Solanum rostratum, has the characteristics of environmental friendliness and strong operability, and has ecological benefits and promotion potential.

[0009] Preferably, the soil containing Solanum rostratum seeds is soil containing Solanum rostratum seeds after the Solanum rostratum plants are uprooted.

[0010] Preferably, the effective viable bacterial count of the liquid bacterial agent is greater than or equal to 600 million / mL. Further preferably, in the liquid bacterial agent containing Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens, the Bacillus subtilis is greater than or equal to 300 million / mL, the Bacillus licheniformis is greater than or equal to 150 million / mL, and the Bacillus amyloliquefaciens is greater than or equal to 150 million / mL.

[0011] Preferably, the soil conditioner further comprises at least one of a solid bacterial fertilizer, an organic fertilizer and an inorganic fertilizer. The solid bacterial fertilizer comprises Bacillus amyloliquefaciens. The organic fertilizer comprises bacterial residue and rice husk powder. The inorganic fertilizer comprises nitrogen (N), P2O5, K2O, boron (B) and zinc (Zn).

[0012] The soil conditioner of the present scheme further comprises at least one of a solid bacterial fertilizer, an organic fertilizer and an inorganic fertilizer, which can further inhibit the seed setting amount (the average number of spiny bracts per Solanum rostratum plant) of Solanum rostratum, reduce the germination amount of Solanum rostratum seeds in the soil, and further reduce the number of original Solanum rostratum seeds existing in the soil, thereby reducing the reproduction amount of Solanum rostratum plants from the root source.

[0013] Preferably, in the organic fertilizer, the bacterial residue is 60-80 parts by mass, and the rice husk powder is 20-40 parts by mass.

[0014] Further preferably, in the organic fertilizer, the bacterial residue is 70 parts by mass, and the rice husk powder is 30 parts by mass.

[0015] As preferred, the mass percentage content of the nitrogen is a, the mass percentage content of the P2O5 is b, the mass percentage content of the K2O is c, the mass percentage content of the boron is d, and the mass percentage content of the zinc is e; wherein a>15.0%, b>5.0%, c>30.0%, d>0.05%, and e≥0.05%; Preferably, 15.0%<a≤25.0%, 5.0%<b≤10.0%, 30.0%<c≤50.0%, 0.05%<d≤0.08%, and 0.05%≤e≤0.10%.

[0016] As preferred, 1-3 L of the liquid microbial agent is applied to each mu of soil; Further preferably, 2-3 L of the liquid microbial agent is applied to each mu of soil.

[0017] As preferred, 80-100 kg of the solid microbial fertilizer is applied to each mu of soil.

[0018] Further preferably, 80 kg of the solid microbial fertilizer is applied to each mu of soil.

[0019] As preferred, 40-60 kg of the organic fertilizer is applied to each mu of soil.

[0020] Further preferably, 50 kg of the organic fertilizer is applied to each mu of soil.

[0021] The present scheme controls the application of 40-60 kg of the organic fertilizer to each mu of soil, which can not only ensure that there is enough of the organic fertilizer to effectively condition the soil, but also avoid excessive application of the organic fertilizer, which can cause unbalanced nutrients or soil acidification, thereby affecting plant growth.

[0022] As preferred, 4-6 kg of the inorganic fertilizer is applied to each mu of soil.

[0023] Further preferably, 5 kg of the inorganic fertilizer is applied to each mu of soil.

[0024] The present scheme controls the application of 4-6 kg of the inorganic fertilizer to each mu of soil, which can not only ensure that there is enough of the inorganic fertilizer to effectively condition the soil, but also avoid excessive application of the inorganic fertilizer, which can cause soil salinization and compaction, thereby affecting plant growth.

[0025] As preferred, the replacement plant is selected from at least one of Medicago polymorpha, Leymus chinensis, Agropyron cristatum, and Festuca rubra. DETAILED DESCRIPTION

[0026] In order to make the objects, technical solutions and advantages of the present application clearer, the technical solutions in the present application will be clearly and completely described below in combination with the embodiments in the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the protection scope of the present application.

[0027] If a specific technology or condition is not specified in the embodiments, it is performed according to the conventional method or the technology or condition described in the literature in the art or according to the product instruction. If the manufacturer of the reagent or instrument used is not specified, it is a conventional product that can be purchased through a regular channel.

[0028] Embodiment 1 The present test is an indoor potting test, and a complete random design is adopted.

[0029] 1. Test materials The plants (A) selected for the test are the invasive plant Solanum rostratum (A1) and the substitute plants: Medicago polymorpha (A2), Leymus chinensis (A3), Agropyron cristatum (A4) and Dactylis glomerata (A5). The Solanum rostratum seeds were collected from Zhangjiakou City, Hebei Province in September 2024 and were stored at 4°C. The seeds were soaked in a 0.083 g / mL gibberellin solution for 24 hours before sowing. The seeds of the substitute plants were purchased from Beijing Goldland Agricultural Technology Institute. The Medicago polymorpha variety is Xinmu No. 2, the Leymus chinensis variety is Zhongke No. 5, the Agropyron cristatum variety is Xuehu, and the Dactylis glomerata variety is Doupai.

[0030] The liquid fungicide (C) used in the test is Xuhuojing No. 1, which is applied at different volume ratios (10 mL (C1), 20 mL (C2) and 30 mL (C3)). It is provided by Beijing Xuhuo International Agricultural Development Co., Ltd.

[0031] The solid fungicide Bujingli 600 million (B1, 80 kg per mu of land), organic fertilizer Fulesi organic fertilizer (B2, 50 kg per mu of land) and inorganic fertilizer Guantiancaixiang 15-5-30 TE (B3, 5 kg per mu of land) used in the test are all provided by Shandong Pangda Biological Group.

[0032] The pot specifications for the potting test are an outer diameter of 16 cm, a height of 13 cm and an inner diameter of 14 cm.

[0033] The soil used for the potting test is collected from the perennial occurrence site of Solanum rostratum in Baicheng City, Jilin Province, and is sieved through a 60-mesh sieve to ensure that the soil in the pots does not contain Solanum rostratum seeds.

[0034] 2. Planting group design The following treatment groups are set up: Control group (CK): A1, A2, A3, A4, A5, A1xA2 to A1xA5 (9 groups in total) • Plant - solid microbial fertilizer, organic fertilizer or inorganic fertilizer interaction group: A1xA1 to A1xA5xB3 (27 groups in total) • Plant - liquid microbial agent interaction group: A1xC1 to A1xA5xC3 (27 groups in total) That is, there are 3 treatment groups in this study, including 1 control group and 2 test groups, and 63 treatment combinations. The control group has 9 treatment combinations, respectively, the invasive plant Solanum rostratum (A1) single species, the replacement plant (A2-A5) single species, and Solanum rostratum (A1) mixed with the replacement plant (A2-A5). The test group includes two types of treatment combinations: the control group and the solid microbial fertilizer, organic fertilizer or inorganic fertilizer interaction group (27 treatment combinations) and the control group and the liquid microbial agent interaction group (27 treatment combinations). The control group and the solid microbial fertilizer, organic fertilizer or inorganic fertilizer interaction group: the control group and 3 types of solid microbial fertilizer, organic fertilizer or inorganic fertilizer (B1-B3) cross-pairing, a total of 27 treatments. The control group and the liquid microbial agent interaction group: the control group and 3 types of liquid microbial agent volume (C1-C3) cross-pairing, a total of 27 treatments. A total of 63 treatment combinations, repeated 5 times, a total of 315 pots.

[0035] 3. Planting treatment method After soaking the Solanum rostratum seeds in 0.083 g / mL gibberellin solution for 24 hours, on April 10, 2025, the seeds were planted in the greenhouse of the Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agricultural Sciences. Solanum rostratum was planted with 10 seeds per pot, and the replacement plant was planted with 30 seeds. During the late seedling stage, the total number of plants in the pot was kept at no more than 24 by removing excess plants. No additional fertilizer was applied during the plant growth period, and regular and quantitative watering was carried out, and weeds in the pot were removed in time. The experiment ended on June 15, 2025 to June 23, 2025, and the aboveground parts of the invasive plants and the replacement plants and the soil samples were collected. The aboveground parts were collected by cutting along the soil surface, counting the number of spiny bracts (fruiting amount) of each Solanum rostratum plant, and then measuring the biomass. Rhizosphere soil samples were collected by the shaking root method for determination of soil physical and chemical properties, and the remaining soil in the pot was used for seed bank determination.

[0036] 4. Index determination method Soil physical and chemical properties: determination of total nitrogen (g / kg), organic matter (g / kg), total phosphorus (g / kg), total potassium (g / kg), alkali-hydrolyzable nitrogen (mg / kg), available potassium (mg / kg), available phosphorus (mg / kg), and pH in the rhizosphere soil of plants. The test results are shown in Table 1.

[0037] Biomass determination: Harvest the above-ground parts of the plants to be tested, place them in pre-numbered envelope bags, and dry them to constant weight in an oven at 60°C. Weigh and record the data. The test results are shown in Table 2.

[0038] Test method for Solanum rostratum seed bank: (1) Set up one seed bank investigation quadrat (S) per pot, with the size of the quadrat being 10 cm x 10 cm; (2) Take three layers of soil samples from the soil seed bank in the quadrat, with the depths being 0-2 cm (layer 1), 2-5 cm (layer 2), and 5-10 cm (layer 3); (3) Remove litter, grass roots, stones, and other impurities from the three layers of soil samples taken from the seed bank quadrat, and place them in different self-sealing bags; (4) Take the soil samples back to the laboratory and first pass them through a 40-mesh sieve. If the target seed individuals are larger than 40 mesh, investigate the upper layer of soil samples. If the target seed individuals are smaller than 40 mesh but larger than 60 mesh, pass the lower layer of soil samples through a 60-mesh sieve (60 mesh is equivalent to the size of flour particles), and investigate the upper layer of soil samples. If the target seed individuals are smaller than 60 mesh, pass the soil samples through a 60-mesh sieve, take 100 grams of soil samples back to the laboratory, and then count them under a microscope (not all soil samples need to be examined; the number of seeds in the samples can be obtained by weight conversion).

[0039] (5) If the soil samples are too wet to investigate the soil seed bank on site, take them back to the laboratory and dry them in a cool place. When the humidity is appropriate, use the method in item 4 to investigate the soil samples.

[0040] (6) Record the number of target seeds obtained by direct counting or microscopic examination in the seed bank investigation table, and convert the area of the quadrat to obtain the number of seeds per square meter of the target species in the community investigation quadrat.

[0041] Fruiting amount: average number of spiny bracts per Solanum rostratum plant.

[0042] The test results for the seed bank and the fruiting amount (average number of spiny bracts per Solanum rostratum plant) of Solanum rostratum are shown in Table 3.

[0043] Table 1 As can be seen from Table 1, compared with no addition of solid bacterial fertilizer, organic fertilizer, inorganic fertilizer or liquid bacterial agent, the main physicochemical indexes such as total nitrogen (g / kg), organic matter (g / kg), total phosphorus (g / kg), total potassium (g / kg), alkaline hydrolysis nitrogen (mg / kg), available potassium (mg / kg) and available phosphorus (mg / kg) are optimized after adding the soil conditioner, which indicates that the addition of the soil conditioner improves the replacement control effect and solves the problem of soil nutrient imbalance caused by overuse of single species of Solanum rostratum.

[0044] Table 2 As can be seen from Table 2, compared with no addition of solid bacterial fertilizer, organic fertilizer, inorganic fertilizer or liquid bacterial agent, the biomass of Solanum rostratum in the replacement control group is significantly reduced after adding the soil conditioner, while the biomass of the replacement plant as a good pasture is significantly increased after adding the soil conditioner, which can effectively inhibit the growth of Solanum rostratum and bring better economic benefits.

[0045] Table 3 As can be seen from Table 3, compared with no addition of solid bacterial fertilizer, organic fertilizer, inorganic fertilizer or liquid bacterial agent, the seed bank and seed setting amount of Solanum rostratum in the replacement control group are significantly reduced after adding the soil conditioner, which indicates that the soil conditioner can significantly improve the replacement control effect and more effectively inhibit the growth and reproduction of Solanum rostratum.

[0046] Example 2 The difference between the indoor pot experiment of this example and Example 1 is as follows: Example 1 is a combination of liquid bacterial agent, solid bacterial fertilizer, organic fertilizer or inorganic fertilizer with replacement plant planting, and the plant-liquid bacterial agent, solid bacterial fertilizer or organic fertilizer interaction group and the plant-liquid bacterial agent interaction group are separated, while Example 2 is a plant-comprehensive conditioning group, which combines the replacement plant planting with liquid bacterial agent and any one of solid bacterial fertilizer, organic fertilizer and inorganic fertilizer, in order to further optimize the technical performance.

[0047] The following treatment groups are set: Blank control group (CK): A1, A2, A3, A4, A5, A1xA2 to A1xA5 (a total of 9 groups) Plant-comprehensive conditioning group: A1xB1xC1 to A1xA5xB3xC3 (a total of 81 groups) That is, the present study has 2 treatment groups, including 1 control group and 1 test group, 90 treatment combinations. The control group has 9 treatment combinations, respectively, the invasive plant Solanum rostratum (A1) single, replacement plants (A2-A5) single and Solanum rostratum (A1) mixed with replacement plants (A2-A5). The test group is the interaction of the control group with liquid inoculum and solid inoculum, organic fertilizer, inorganic fertilizer: the control group is crossed with solid inoculum, organic fertilizer, inorganic fertilizer (B1-B3) and 3 kinds of liquid inoculum volume (C1-C3), a total of 81 kinds of treatment. A total of 90 treatment combinations, repeated 5 times, a total of 450 pots.

[0048] Table 4 Example 2 pot experiment Solanum rostratum seed bank and seed set As can be seen from Table 4, compared with the control group, the control group + solid inoculum, organic fertilizer or inorganic fertilizer, the single combination of the control group + liquid inoculum, the seed bank and seed set of Solanum rostratum in the plant + comprehensive conditioning group are further reduced, which shows that the replacement control under comprehensive conditioning can more effectively reduce the growth and reproduction rate of Solanum rostratum.

[0049] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, but not to limit it; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for controlling the invasion of *Solanum nigrum*, characterized in that, Includes the following steps: Plant alternative plant seeds in soil containing Solanum spinosa seeds, and apply soil conditioner simultaneously; The soil conditioner includes a liquid bacterial agent; The liquid bacterial agent includes Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens.

2. The method for controlling the invasion of *Solanum nigrum* according to claim 1, characterized in that, The effective viable count of the liquid bacterial agent is ≥600 million CFU / mL; Preferably, in the liquid bacterial agent comprising Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens, the Bacillus subtilis is ≥300 million CFU / mL, the Bacillus licheniformis is ≥150 million CFU / mL, and the Bacillus amyloliquefaciens is ≥150 million CFU / mL.

3. The method for controlling the invasion of *Solanum nigrum* according to claim 1 or 2, characterized in that, The soil conditioner also includes at least one of solid microbial fertilizer, organic fertilizer, and inorganic fertilizer; The solid microbial fertilizer includes Bacillus amyloliquefaciens; The organic fertilizer includes mushroom residue and rice husk powder; The inorganic fertilizer includes nitrogen, P2O5, K2O, boron, and zinc.

4. The method for controlling the invasion of *Solanum nigrum* according to claim 3, characterized in that, By weight, the organic fertilizer contains 60-80 parts of the fungal residue and 20-40 parts of the rice husk powder.

5. The method for controlling the invasion of *Solanum nigrum* according to claim 3 or 4, characterized in that, In the inorganic fertilizer, let the mass percentage of nitrogen be a, the mass percentage of P2O5 be b, the mass percentage of K2O be c, the mass percentage of boron be d, and the mass percentage of zinc be e; wherein a > 15.0%, b > 5.0%, c > 30.0%, d > 0.05%, and e ≥ 0.05%; Preferably, 15.0% < a ≤ 25.0%, 5.0% < b ≤ 10.0%, 30.0% < c ≤ 50.0%, 0.05% < d ≤ 0.08%, and 0.05% ≤ e ≤ 0.10%.

6. The method for controlling the invasion of *Solanum nigrum* according to any one of claims 1 to 5, characterized in that, Apply 1-3 L of the liquid bacterial agent per acre of soil; Preferably, 2-3 L of the liquid bacterial agent is applied per acre of soil.

7. The method for controlling the invasion of *Solanum nigrum* according to claim 3 or 6, characterized in that, Apply 80-100 kg of the solid microbial fertilizer per acre of soil.

8. The method for controlling the invasion of *Solanum nigrum* according to claim 3, 4, or 7, characterized in that, Apply 40-60 kg of the organic fertilizer described above per acre of soil.

9. The method for controlling the invasion of *Solanum nigrum* according to claim 3, 5, or 8, characterized in that, Apply 4-6 kg of the aforementioned inorganic fertilizer per acre of soil.

10. The method for controlling the invasion of *Solanum nigrum* according to claim 1 or 9, characterized in that, The alternative plant is selected from at least one of alfalfa, sheepgrass, flat-spike grass, and reed fescue.

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