Orifice-opening and lung-ventilating essential oil and preparation method thereof

Abstract

The invention discloses essential oil capable of opening orifices and freeing lung and a preparation method of the essential oil. The essential oil is prepared from the following raw materials in parts by weight: 40-70 parts of sweet almond oil, 10-30 parts of refined jojoba oil, 2-10 parts of cuba balsam essential oil, 1-5 parts of neroli essential oil, 1-5 parts of sweet laurel essential oil, 1-5 parts of peppermint essential oil, 1-5 parts of eucalyptus australis essential oil, 1-5 parts of fir essential oil, 1-3 parts of bitter orange essential oil, 1-4 parts of magnolia flower essential oil and 1-4 parts of purple perilla essential oil. The essential oil is prepared from natural plant extracts, has the effects of opening orifices and freeing lung, greatly reduces the risk of adverse reaction on human bodies, and is suitable for long-term use by adults.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of medicine, and particularly relates to a Qunqiaoxuanfei essential oil and a preparation method thereof. BACKGROUND

[0002] Nasal congestion, runny nose, sneezing, nasal itching, decreased olfaction, dizziness and head swelling are common symptoms of the upper respiratory tract, which are caused by inflammation, foreign bodies, physical or chemical stimulation of the nasal mucosa, and bring intense discomfort and pain to patients. With the passage of time, many acute rhinitis caused by inflammation turns into chronic rhinitis, which often brings great pain to patients, such as nasal congestion, olfactory dysfunction, post-nasal drip and the like.

[0003] At present, there are many drugs and methods in traditional Chinese medicine and Western medicine to treat rhinitis, but each of them has some shortcomings.

[0004] Western medicine usually uses antiallergic and anti-inflammatory drugs to treat rhinitis, and long-term use of drugs may lead to increased drug resistance and drug side effects, and long-term use of drugs may reduce efficacy.

[0005] Traditional Chinese medicine uses the method of syndrome differentiation and treatment to regulate the state of qi, blood, yin and yang of the body to achieve the purpose of treating rhinitis.

[0006] Essential oils are pure natural, safe, easy to absorb, convenient to use and the like, but there is a lack of essential oils with exact and significant therapeutic effect for treating rhinitis on the market at present. SUMMARY

[0007] The purpose of the present application is to provide a Qunqiaoxuanfei essential oil and a preparation method thereof, which uses natural plant extracts, greatly reduces the risk of adverse reactions to the human body while achieving the effect of Qunqiaoxuanfei, and is suitable for long-term use by adults.

[0008] To achieve the above purpose, the technical scheme of the present application is as follows: A Qunqiaoxuanfei essential oil comprises the following components by weight: 40-70 parts of sweet almond oil, 10-30 parts of refined jojoba oil, 2-10 parts of Cuban balsam essential oil, 1-5 parts of orange flower essential oil, 1-5 parts of sweet bay essential oil, 1-5 parts of black leaf mint essential oil, 1-5 parts of eucalyptus essential oil, 1-5 parts of fir essential oil, 1-3 parts of bitter orange essential oil, 1-4 parts of magnolia essential oil, and 1-4 parts of perilla essential oil. The preparation method of the Qunqiaoxuanfei essential oil comprises the following steps: a. Take equal mass of cuban balsam, orange flower, sweet bay, black leaf mint, eucalyptus globulus, fir essential oil, wash them clean and crush them respectively to obtain corresponding plant solid fragments, and then extract each plant solid fragment by using steam distillation method, filter the extracted product and collect the filtrate to obtain cuban balsam essential oil, orange flower essential oil, sweet bay essential oil, black leaf mint essential oil, eucalyptus globulus essential oil, fir essential oil, lily magnolia essential oil and perilla essential oil respectively; b. Take equal mass of bitter orange peel as the raw material in step a), wash it clean, and then extract it by using cold pressing method, wherein the temperature during the extraction process is controlled at 5-10℃ to obtain bitter orange essential oil; c. Take equal mass of sweet almond as the raw material in step a), shell and wash it, then crush it into paste, heat it at a temperature of 60-80℃ for extraction, obtain oil, remove the solvent by distillation, and then perform mucilage removal, acidification and decolorization treatment, and finally perform high-temperature vacuum distillation at a temperature of 240-280℃, filter the extracted product and collect the filtrate to obtain sweet almond oil; d. Take equal mass of jojoba seed as the raw material in step a), wash it clean, and then extract it by cold pressing method, filter the residue to obtain refined jojoba oil; e. Mix the cuban balsam essential oil, orange flower essential oil, sweet bay essential oil, black leaf mint essential oil, eucalyptus globulus essential oil, fir essential oil, bitter orange essential oil, sweet almond oil, lily magnolia essential oil, perilla essential oil and refined jojoba essential oil obtained above to obtain an essential oil composition.

[0009] Preferably, in step a), when each plant essential oil is extracted by using steam distillation method, the mass ratio of water to plant solid fragments is 4-6:1.

[0010] Preferably, in step d), the temperature during the cold pressing process is 40-60℃, the cold pressing pressure is 20-30MPa, and the extraction time is 30-60 minutes.

[0011] The essential oil for dredging the nasal cavity and promoting lung function has the properties of anti-inflammatory and antibacterial of cuban balsam, can relieve the nasal congestion and runny nose caused by rhinitis, and can regulate the respiratory system as a whole to achieve more comprehensive and longer-lasting dredging and promoting lung function.

[0012] The coordinated treatment effect of the essential oil for dredging the nasal cavity and promoting lung function is achieved by the mucous membrane protection effect of the base oil and the synergistic effect of the anti-inflammatory, antibacterial, dredging and soothing effects of various essential oils on the core pathological links of rhinitis (nasal mucosa inflammation, hyperemia and edema, sensitive stimulation, mucus obstruction, etc.). I. Core pathological links and corresponding component-specific effects The main pathological manifestations of rhinitis include: chronic inflammation of the nasal mucosa (redness, exudation), congestion leading to nasal congestion, mucosal sensitivity to irritation (sneezing, runny nose), decreased local immune function, and secondary infection. The components of the essential oil described in the present application precisely exert force from four dimensions: repairing the mucosal barrier, anti-inflammatory and antibacterial, clearing the nasal passages and removing turbidity, and soothing sensitivity: 1. Base oil: repair the mucosal barrier and reduce irritation Sweet almond oil: as the main base oil, it is mild in texture and rich in unsaturated fatty acids (such as oleic acid, linoleic acid) and vitamin E, which can moisturize the nasal mucosa and the skin around the nose, repair dry and damaged mucosal barriers, reduce damage to the nasal mucosa from external stimuli (such as cold air, dust), and reduce the risk of rhinitis induction or exacerbation.

[0013] Refined jojoba oil: contains natural vitamin E and phytol, has a light texture and a molecular structure similar to human skin, is highly skin-friendly and low in allergenicity, can enhance the penetration rate of fat-soluble essential oils, supplement the moisture of the nasal mucosa, improve the barrier's water retention and repair efficiency, and is suitable for people with fragile nasal mucosa. During the cold pressing process, the temperature is controlled at 40-60°C to avoid decomposition of phytol in jojoba oil above 60°C.

[0014] 2. Anti-inflammatory and antibacterial: directly attacking the causes of inflammation and infection Nasal inflammation (especially chronic rhinitis and allergic rhinitis) is often accompanied by chronic inflammation of the nasal mucosa, or exacerbated by bacterial / viral infection. Various essential oils reduce the release of inflammatory mediators through anti-inflammatory and antibacterial effects, and control infection: Cuban balsam essential oil: the core component is sesquiterpenes (such as caryophyllene), which has significant anti-inflammatory activity and can inhibit the release of inflammatory factors (such as IL-6, TNF-α) in the nasal mucosa, reducing mucosal redness; at the same time, it has a broad antibacterial spectrum and can inhibit common respiratory pathogens such as Staphylococcus aureus, reducing the risk of infection.

[0015] Sweet bay essential oil: contains eucalyptol, linalool, etc., used for respiratory care, has antibacterial and antifungal effects, can reduce the colonization of pathogenic microorganisms on the nasal mucosa; at the same time, it can promote mucus dilution and help relieve nasal congestion.

[0016] Australian eucalyptus essential oil: rich in 1,8-cineole, not only has strong antibacterial (against bacteria, viruses) and antifungal effects, but also can reduce congestion by inhibiting the inflammatory response of the nasal mucosa, while stimulating cilia movement to accelerate mucus discharge and reduce nasal obstruction.

[0017] Perilla essential oil: contains perilla alcohol and limonene, which has inhibitory effects on Streptococcus pneumoniae and influenza virus, can enhance the antibacterial effect of the composition; at the same time, its anti-inflammatory properties can reduce nasal mucosal exudation and help relieve the nasal itching and runny nose caused by allergic rhinitis.

[0018] 3. Clearing the nasal passages and removing turbidity: quickly relieving nasal congestion and mucus blockage Nasal congestion is one of the most troublesome symptoms of rhinitis, associated with nasal mucosal hyperemia and excessive mucus secretion. The composition relieves through the dual effects of "clearing the nasal passages + promoting removal": Black mint essential oil: contains menthol, menthone and other components, has a strong cooling sensation, can stimulate the cold receptors of the nasal mucosa, temporarily constrict the nasal mucosal blood vessels, and quickly relieve nasal congestion; at the same time, it is highly volatile and can transmit soothing signals through the olfactory nerve, reducing symptoms such as nasal itching and sneezing.

[0019] Fir essential oil: contains terpenes such as α-pinene and β-pinene, has the traditional effect of "promoting lung and clearing the nasal passages", can relax the smooth muscles of the respiratory tract, promote the discharge of mucus in the nasal cavity and sinuses, and reduce the aggravation of nasal congestion caused by mucus retention; at the same time, its anti-inflammatory effect can help reduce nasal mucosal edema.

[0020] Magnolia essential oil: contains citronellol and eugenol, traditionally used to improve nasal ventilation, can directly act on the nasal mucosa to reduce inflammation-induced mucosal swelling, and at the same time assist in repairing the olfactory pathway and improving olfactory decline symptoms.

[0021] 4. Relieving sensitivity: reducing nasal mucosa hypersensitivity Patients with allergic rhinitis or chronic rhinitis often have sensitive nasal mucosa (easily overreacting to pollen, dust, etc.), which manifests as frequent rhinorrhea and sneezing. The essential oils described in the invention reduce sensitivity through soothing and sedative ingredients.

[0022] Orange flower essential oil: contains methyl anthranilate and linalool, has a sedative and anti-anxiety effect, can regulate the nervous system through the olfactory pathway, reduce the sensitivity of the nasal mucosa to external stimuli; at the same time, its anti-inflammatory effect can reduce histamine-mediated allergic reactions (such as histamine-induced vasodilation and rhinorrhea).

[0023] Bitter orange essential oil: contains hesperidin and limonene, has antioxidant and anti-inflammatory effects, can inhibit the release of allergic mediators (such as histamine) from mast cells, and reduce nasal itching and rhinorrhea caused by allergic reactions; its mild citrus aroma can also relax the respiratory tract and help improve respiratory congestion.

[0024] II. Multiple components synergize: form a closed-loop treatment of "protection - anti-inflammatory - clearing the nasal passages - soothing" The coordinated effect of the nasal passage clearing and lung clearing essential oil described in the invention is not the effect of a single component alone, but a logical chain of repairing the barrier with base oil → controlling inflammation and infection with antibacterial and anti-inflammatory components → relieving nasal congestion with clearing components → reducing sensitivity with soothing components, forming a "closed-loop intervention" for rhinitis pathology.

[0025] Base oil (sweet almond oil, refined jojoba oil) provides physical protection to the mucosa, reducing the irritation of essential oils to sensitive mucosa, while enhancing the penetration and absorption of other fat-soluble essential oil components; base oil and black leaf mint essential oil synergistically control release, avoiding excessive vasoconstriction causing mucosal ischemic injury.

[0026] Cuba balsam, laurel, eucalyptus, perilla inhibit inflammation and infection from the "root", reducing the persistent inducement of nasal mucosa injury; and the long-chain fatty acid esters in refined jojoba oil synergize with Cuba balsam, both of which can broaden the antibacterial spectrum and enhance anti-inflammatory activity. In the case of adding the same amount, the combination use can improve the anti-infection ability by more than 50% compared with single use.

[0027] Black leaf mint, gum fir quickly "symptomatic" relieve nasal congestion, improve respiratory patency, and improve patient comfort; Orange flower, bitter orange, linalool in orange flower can regulate nerve ending sensitivity, and hesperidin in bitter orange can inhibit mast cell histamine release. The combination of the two can reduce mucosal sensitivity from the "neural regulation - immune suppression" dual-path level, reduce symptom recurrence, and promote nasal mucosa function recovery with the repair effect of base oil. The combination of orange flower and bitter orange can inhibit the release rate of histamine and synergistically improve the anti-allergic effect, which is more than 80% higher than the single use.

[0028] This essential oil composition, through the synergistic effect of multiple components and multiple targets, not only interferes with the core pathology of rhinitis (inflammation, infection, congestion, and sensitivity), but also considers mucosa repair and symptom relief, achieving a coordinated treatment effect of "treating inflammation, unblocking, protecting membranes, and relieving", thereby assisting in improving symptoms such as nasal congestion, nasal itching, and runny nose caused by rhinitis.

[0029] Different plant materials are extracted by precise adaptation methods. Cuba balsam, orange flower, etc. are extracted by steam distillation method, and the mass ratio of water to plant solid fragments is strictly controlled at 4-6:1. This strict control of the mass ratio of water to plant solid fragments during essential oil production is to efficiently retain key ingredients and ensure stable efficacy, with the following specific effects: 1. Ensure complete extraction of ingredients A high ratio (too much water) will prolong the distillation time, causing the loss of volatile components such as inflammation-fighting ingredients in Cuba balsam (e.g., caryophyllene) and menthol in black leaf mint due to prolonged heating; a low ratio (insufficient water) will cause local overheating of the plant, damaging heat-sensitive components such as eucalyptol in Australian eucalyptus and linalool in orange flower, weakening the anti-inflammatory and unblocking effects.

[0030] 2. Improve extraction efficiency and purity The ratio of water to plant solid fragments is 4-6:1, which can fully soak the plant fragments, uniformly carry the essential oil components by steam, reduce the mixing of impurities, ensure the effective extraction of the antibacterial components of sweet laurel, the turbidity removing components of cold fir, and guarantee the concentration of the core components of "anti-inflammatory, nasal passage clearing, and soothing", thereby maintaining the synergistic therapeutic effect on rhinitis.

[0031] The bitter orange essential oil is obtained by cold pressing using the peel of bitter oranges, and the temperature during the extraction process is controlled at 5-10℃, so as to ensure that the effective components and activities of the essential oil are maximally retained.

[0032] During the extraction of sweet almond oil and fractionated coconut oil, the purity and stability of the products are significantly improved through specific temperature control and multi-channel processing technology, so as to guarantee the quality and efficacy of the essential oil.

[0033] The nasal passage clearing and lung opening essential oil disclosed by the application overcomes the following defects of the current existing technology for relieving rhinitis essential oil: 1. Overcoming the problem of strong irritability and insufficient adaptability of the base oil: some essential oils on the market use poor base oil without refining, or the proportion of the base oil is unbalanced, which is easy to cause mucous membrane or skin irritation (such as redness and burning) due to impurity residues and poor skin affinity. In the application, high proportion of sweet almond oil and refined jojoba oil are used as the base, both of which are natural and mild high-quality base oils with light texture and strong skin affinity, which can effectively dilute the concentration of single essential oil, reduce the risk of irritation to the respiratory tract and skin, and are more suitable for delicate parts (such as the nasal cavity and the surrounding throat) that need long-term contact, thereby reducing the risk of discomfort or allergy.

[0034] 2. Overcoming the problem of single component and limited efficacy: most of the essential oils for clearing the nasal passage or lung care on the market have single components, which can only target a single symptom (such as temporary nasal passage clearing or slight soothing), and it is difficult to achieve the synergistic effect of "clearing the nasal passage" and "opening the lung". The formula of the application uses multiple essential oils with efficacy: Cuban balsam essential oil for anti-inflammatory repair, black leaf mint, Australian eucalyptus, and magnolia for clearing the nasal passage and clearing the turbidity, cold fir essential oil and sweet laurel, perilla for lung opening and regulating qi, orange flower and bitter orange for soothing and moisturizing. Through the multi-dimensional synergy of "clearing the nasal passage, opening the lung, repairing, and soothing", the core needs of respiratory discomfort are covered, and the limitations of single component efficacy and poor synergy are overcome.

[0035] 3. Overcoming the problem of insufficient safety and high risk of long-term use: some essential oils on the market add synthetic fragrances, preservatives and other chemical components in pursuit of "quick effect", or the concentration of single essential oil is too high, which can easily cause mucous membrane sensitivity, allergy and other adverse reactions during long-term use, and it is difficult to meet the long-term care needs. The formula clearly uses "only natural plant extracts", without chemical additives, and by reasonably controlling the concentration of single essential oil and the synergy of high proportion of base oil, the irritability is reduced while the efficacy is guaranteed, solving the problem of "short-term use of essential oils on the market, long-term use of skin / mucous membrane" and realizing the advantages of "mild care and suitable for long-term use".

[0036] 4. Overcome the problem of unclear efficacy orientation and poor targeting: Many essential oils on the market are positioned as "general maintenance" and are not specifically designed for "clearing and relieving lung" scenarios. The efficacy is generalized (such as only marked "relieving" and "refreshing"), which makes it difficult to accurately improve problems such as nasal congestion, breathing difficulty, and lung discomfort. The formula of the present application takes "clearing and relieving lung" as the core target, selects components with clear efficacy orientation (such as Eucalyptus clearing, Abies relieving lung, and Sweet Laurel regulating qi), and matches the respiratory care needs in a targeted manner, overcoming the shortcomings of similar products such as "unclear efficacy" and "difficult to choose as needed".

[0037] 5. Overcome the problem of low purity of natural ingredients and discounted efficacy: Some essential oils on the market have low proportion of natural ingredients and are mixed with cheap synthetic substitutes, resulting in a discrepancy between actual efficacy and promotion. The formula of the present application emphasizes the use of natural plant extracts to ensure the purity and activity of natural ingredients, overcoming the problem of impure natural ingredients and false labeling of efficacy of essential oils on the market.

[0038] Advantages of the present application: The essential oil of the present application can effectively act on the respiratory system of the human body by scientifically blending multiple natural plant essential oils and synergistically acting multiple plant essential oil components. It not only can relieve symptoms such as nasal congestion and runny nose caused by rhinitis, but also can regulate the respiratory system as a whole, achieving more comprehensive and longer-lasting clearing and relieving lung effect. Each component is a natural plant extract, which greatly reduces the risk of adverse reactions to the human body, is safe and has no side effects, and is suitable for long-term use by adults.

[0039] The essential oil obtained by the present application can be treated in various forms such as flushing, sniffing, and rubbing for external use, and has excellent effect of relieving rhinitis. It can also be supplemented with conventional drug therapy of traditional Chinese medicine and Western medicine, shorten the course of disease, and reduce the amount of drug intake by patients.

[0040] In the present application, suitable extraction methods are used when extracting plant essential oils from different plant raw materials, which maximizes the retention of effective components of each plant and ensures the quality of the essential oil composition. BRIEF DESCRIPTION OF DRAWINGS

[0041] Figure 1 Comparison of histopathology pictures of nasal mucosa of each group of rat models; Figure 2 Influence control chart of SYK / PI3K / AKT1 signal pathway of nasal mucosa of each group of rat models. DETAILED DESCRIPTION

[0042] Example 1 A method for preparing a clearing and relieving lung essential oil composition, comprising the following steps: a. Take 100g of each of cuban balsam, orange flower, sweet bay, black leaf mint, eucalyptus, fir, magnolia bud, perilla stem and leaf, wash them clean and crush them separately to obtain corresponding plant solid fragments, extract each plant solid fragment separately by using water vapor distillation method, filter and collect the filtrate to obtain cuban balsam essential oil, orange flower essential oil, sweet bay essential oil, black leaf mint essential oil, eucalyptus essential oil, fir essential oil, magnolia essential oil, and perilla essential oil respectively; the mass ratio of water to plant solid fragments during extraction is 4:1; b. Take 100g of bitter orange peel, wash it clean, and extract it by cold pressing method, with the temperature controlled at 5℃ during extraction, to obtain bitter orange essential oil; c. Take 100g of sweet almond, crush it into paste after shelling and washing, and extract it by heating at 60℃, to obtain oil, which is then subjected to mucilage removal, acidification and decolorization treatment after removal of solvent by distillation, and finally high-temperature vacuum distillation at a temperature of 240℃, filter and collect the filtrate to obtain sweet almond oil; d. Take 100g of jojoba seeds, wash them clean, and extract jojoba oil by cold pressing method, with the cold pressing pressure being 20MPa and the temperature being 40℃, and the extraction time being 30 minutes; e. Mix 40 parts of sweet almond oil, 10 parts of refined jojoba oil, 2 parts of cuban balsam, 1 part of orange flower essential oil, 1 part of sweet bay essential oil, 1 part of black leaf mint essential oil, 1 part of eucalyptus essential oil, 1 part of fir essential oil, 1 part of bitter orange essential oil, 1 part of magnolia essential oil, and 1 part of perilla essential oil obtained above to obtain an essential oil composition.

[0043] Example 2 A preparation method of an essential oil composition for unblocking collaterals and relieving lung, comprising the following steps: a. Take 150g of each of cuban balsam, orange flower, sweet bay, black leaf mint, eucalyptus, and fir essential oil, wash them clean and crush them separately to obtain corresponding plant solid fragments, extract each plant solid fragment separately by using water vapor distillation method, filter and collect the filtrate to obtain cuban balsam essential oil, orange flower essential oil, sweet bay essential oil, black leaf mint essential oil, eucalyptus essential oil, and fir essential oil respectively; the mass ratio of water to plant solid fragments during extraction is 5:1; b. Take 150g of bitter orange peel, wash it clean, and extract it by cold pressing method, with the temperature controlled at 8℃ during extraction, to obtain bitter orange essential oil; c. Take 150g of sweet almond, crush it into paste after shelling and washing, and extract it by heating at 70℃, to obtain oil, which is then subjected to mucilage removal, acidification and decolorization treatment after removal of solvent by distillation, and finally high-temperature vacuum distillation at a temperature of 260℃, filter and collect the filtrate to obtain sweet almond oil; d. Take 150 g of jojoba seeds, clean them, and extract jojoba oil by cold pressing at a pressure of 25 MPa and a temperature of 50℃ for 45 minutes; e. Mix the sweet almond oil obtained above (70 parts), coconut oil (30 parts), Cuban balsam (10 parts), orange flower oil (5 parts), sweet bay oil (5 parts), black leaf mint oil (5 parts), eucalyptus oil (5 parts), fir oil (5 parts), and bitter orange oil (3 parts) to obtain an essential oil composition.

[0044] Example 3 A method for preparing an essential oil composition for unblocking the channels and promoting lung function, comprising the following steps: a. Take 200 g of Cuban balsam, orange flower, sweet bay, black leaf mint, eucalyptus, and fir oils, respectively, clean them, and crush them to obtain corresponding plant solid fragments. Extract each plant solid fragment by steam distillation, filter the extracted product, and collect the filtrate to obtain Cuban balsam oil, orange flower oil, sweet bay oil, black leaf mint oil, eucalyptus oil, and fir oil, respectively. The mass ratio of water to plant solid fragments during extraction is 6:1. b. Take 200 g of bitter orange peels, clean them, and extract them by cold pressing at a temperature of 10℃ to obtain bitter orange oil. c. Take 200 g of sweet almonds, shell and clean them, crush them into a paste, heat them at a temperature of 80℃ to extract oil, remove the solvent by distillation, and then perform mucilage removal, acidification, and decolorization. Finally, perform high-temperature vacuum distillation at a temperature of 280℃, filter the extracted product, and collect the filtrate to obtain sweet almond oil. d. Take 200 g of jojoba seeds, clean them, and extract jojoba oil by cold pressing at a pressure of 30 MPa and a temperature of 60℃ for 60 minutes. e. Mix the sweet almond oil obtained above (70 parts), coconut oil (30 parts), Cuban balsam (10 parts), orange flower oil (5 parts), sweet bay oil (5 parts), black leaf mint oil (5 parts), eucalyptus oil (5 parts), fir oil (5 parts), and bitter orange oil (3 parts) to obtain an essential oil composition.

[0045] Comparative Example 1 The difference from Example 2 of the present application is that no Cuban balsam oil is added, and the raw material composition of each component of the essential oil includes sweet almond oil (55 parts), refined jojoba oil (26 parts), orange flower oil (3 parts), sweet bay oil (3 parts), black leaf mint oil (3 parts), eucalyptus oil (3 parts), fir oil (3 parts), bitter orange oil (2 parts), lily magnolia oil (1 part), and perilla oil (1 part).

[0046] Comparative Example 2 The difference between the present application embodiment 2 is that no orange flower oil is added, and the raw material composition of each component of the essential oil includes 55 parts of sweet almond oil, 20 parts of refined jojoba oil, 6 parts of cuban balsam essential oil, 3 parts of sweet bay essential oil, 3 parts of black leaf mint essential oil, 3 parts of eucalyptus essential oil, 3 parts of fir essential oil, 5 parts of bitter orange essential oil, 2.5 parts of magnolia essential oil, and 2.5 parts of perilla essential oil.

[0047] Comparative example 3 The difference between the present application embodiment 2 is that no orange flower oil is added, and the raw material composition of each component of the essential oil includes 55 parts of sweet almond oil, 20 parts of refined jojoba oil, 6 parts of cuban balsam essential oil, 3 parts of sweet bay essential oil, 3 parts of black leaf mint essential oil, 3 parts of eucalyptus essential oil, 3 parts of fir essential oil, 5 parts of bitter orange essential oil, 2.5 parts of magnolia essential oil, and 2.5 parts of perilla essential oil.

[0048] Animal experiment 1. Preparation of animal model and grouping of administration 1.1 Experimental animals and grouping Adaptive feeding: 108 healthy SPF level SD rats (body weight 200-220g, half male and half female) were selected and adaptively fed in an environment with temperature (22±2) ℃, humidity (50±10) %, and 12h day-night alternation for 7d, with free feeding and drinking.

[0049] Grouping treatment: after the adaptive feeding, 12 were randomly selected as normal control group (without any sensitization treatment), and the remaining 60 were used to establish an allergic rhinitis (AR) model.

[0050] 1.1.1 Establishment of AR model —— basic sensitization stage Preparation of sensitization suspension: 0.3mg of ovalbumin (OVA) and 30mg of aluminum hydroxide adjuvant (Al (OH)3) were added to 1mL of normal saline, and fully vortexed to mix uniformly to prepare OVA-Al (OH)3 suspension.

[0051] Sensitization operation steps: injection method: intraperitoneal injection (ip), injection frequency: once every other day (i.e. once every 48h), injection times: a total of 7 times, cycle: continuously for 14d (injection on the 1st, 3rd, 5th, 7th, 9th, 11th, and 13th day) Control group treatment: the normal control group rats were injected with the same volume (1mL) of normal saline at the same period, with the same injection frequency and cycle as the model group, to exclude the influence of injection operation on the experimental results.

[0052] 1.1.2 Establishment of AR model —— nose drop challenge stage Preparation of challenge solution: 2% OVA normal saline solution and 1% OVA normal saline solution were prepared and ready for use.

[0053] The priming operation step is as follows: first nose drop priming: on the 2nd day after the end of the basic sensitization (i.e. the 15th day of the experiment), the rats for establishing the AR model are subjected to nose drop, 50 μL of 2% OVA saline solution is dropped into each nostril, 1 time / day, for 7 consecutive days. Maintenance of intranasal priming: after grouping and administration, the model group rats are subjected to nose drop with 1% OVA solution every other day (i.e. on the 22nd, 24th, 26th, 28th, 30th, 32nd and 34th day), 50 μL for each nostril, to maintain the intranasal priming state, simulating the state of daily contact with allergens of AR patients, until the end of the experiment.

[0054] Control group treatment: the normal control group is subjected to nose drop with the same volume of saline, synchronously with the operation of the model group.

[0055] 1.1.3 Model success determination and grouping administration Model success determination: within 30 minutes after the last nose drop, the rats are observed for behavior, and the conditions of scratching the nose, sneezing and running nose are recorded, and the integral ≥ 6 is calculated as model success.

[0056] Model condition evaluation Observation time: within 30 minutes after the last nose drop priming.

[0057] Observation index: the conditions of scratching the nose, sneezing, running nose and nose bleeding of the rats are observed and recorded.

[0058] Scoring standard: the quantitative integral method is adopted, and the specific scoring details are shown in Table 1.

[0059] Model success determination: single or cumulative integral ≥ 6 is determined as AR model establishment success; if the rats scratch the nasal cavity due to severe itching of the nose, the single item is directly counted as 3.

[0060] Table 1 Grouping: the successfully modeled AR rats are randomly divided into a model control group, a loratadine 0.9 m / kg group, an example 1 group, an example 2 group, an example 3 group, a comparative example 1 group, a comparative example 2 group, a comparative example 3 group, 12 rats in each group, and the normal control group is not subjected to any sensitization treatment.

[0061] Administration: starting from the 22nd day, each group is given response treatment, the normal control group and the model control group are given distilled water by gavage, the loratadine group is given gavage treatment, and the administration volume is 10 mL / kg, for 7 consecutive days. The example 1 group, the example 2 group, the example 3 group, the comparative example 1 group, the comparative example 2 group and the comparative example 3 group are respectively given the essential oil nasal irrigation corresponding to the example 1, 2, 3 and comparative example 1, 2, 3 of the application, for 7 consecutive days.

[0062] Sampling: 24 hours after the last treatment, the rats in each group are anesthetized and sacrificed, and the samples are taken for examination.

[0063] 1.2 Detection index 1.2.1 After the last treatment for 24h, the rats were anesthetized by intraperitoneal injection of 20% urethane 1.4g / kg, and then blood was taken from abdominal aorta for 10mL. The blood was left to stand for 1~2h in the room, and then low-temperature centrifugation was carried out at a speed of 3500r / min for 15min, and the serum was collected and stored in a-80℃ refrigerator for detection. The contents of IFN-γ, IL-4, IL-6, IL-17 and TNF-α in the serum were detected by ELISA method, and the specific steps were carried out according to the kit instructions.

[0064] 1.2.2 HE staining to check the nasal mucosa histopathology: after the blood was taken from the abdominal aorta of the rats, the whole head of the rats was taken, the rat skull was stripped of the outer skin and fur, the superior turbinate and nasal maxilla were separated using a surgical knife and surgical scissors, the bilateral nasal cavity was fully exposed, the nasal septum cartilage tissue and mucosa were taken, and were fixed in 10% paraformaldehyde, paraffin-embedded, HE stained, and the nasal mucosa was observed under a light microscope.

[0065] 1.2.3 qRT-PCR method for detecting the expression of Syk, Pik3ca, Pik3cg, Akt1, Prkca, Mapk1, Mapk8, Mapk14, Pla2g4a, Alox5, Alox5ap mRNA in nasal mucosa tissue: the frozen rat nasal mucosa was taken, total RNA was extracted according to the total RNA extraction kit (centrifugal column type) instructions, and the RNA concentration and purity were detected. The RNA was reversely transcribed into cDNA using a reverse transcription kit, and the SYBR Green method was used for three-step real-time fluorescent quantitative PCR, and the expression of spleen tyrosine kinase (Syk), phosphatidylinositol 3-kinase alpha (Pik3ca), phosphatidylinositol 3-kinase gamma (Pik3cg), protein kinase B1 (Akt1), protein kinase C (Prkca), mitogen-activated protein kinase 1 (Mapk1), mitogen-activated protein kinase 8 (Mapk8), mitogen-activated protein kinase 14 (Mapk14), phospholipase A2 (Pla2g4a), polyunsaturated fatty acid 5-lipoxygenase (Alox5), arachidonic acid 5-lipoxygenase activating protein (Alox5ap) mRNA in nasal mucosa tissue was detected. The primers were designed by Primer Premier 6 software and verified by Lasergene software, and the verified primer sequences were synthesized by Shanghai Jeery Biological Engineering Co., Ltd., and the primer sequences are shown in Table 2.

[0066] The three-step reaction conditions are: pre-denaturation at 95°C for 2 min, reaction at 95°C for 5 s, annealing at 56°C for 10 s, extension at 72°C for 15 s, and cycling 40 times. Gapdh is used as an internal reference, and each group of experimental biological repeats 3 times, and the relative expression of the target gene is calculated using the 2-△△Ct method.

[0067] Table 2 1.2.4 Western blot method for detecting nasal mucosa related protein expression: the frozen rat nasal mucosa was taken, and the total protein of the nasal mucosa was extracted according to the total protein extraction kit instruction. The loading amount was measured by BCA protein quantification method. The proteins were separated by SDS-PAGE electrophoresis, and the appropriate PVDF membrane was cut and soaked in methanol for 3 min, and then transferred to the PVDF membrane. After the membrane was transferred, the membrane was placed in 5% skim milk solution and shaken for 2 h. After blocking, the membrane was placed in the primary antibody solution of GAPDH (1:5000), p-PI3K (1:500), PI3K (1:500), p-Syk (1:500), Syk (1:1000), p-AKT1 (1:500), and AKT1 (1:500) at 4°C overnight.

[0068] 2 Experimental results 2.1 Influence of the nasal mucosa related protein expression: the frozen rat nasal mucosa was taken, and the total protein of the nasal mucosa was extracted according to the total protein extraction kit instruction. The loading amount was measured by BCA protein quantification method. The proteins were separated by SDS-PAGE electrophoresis, and the appropriate PVDF membrane was cut and soaked in methanol for 3 min, and then transferred to the PVDF membrane. After the membrane was transferred, the membrane was placed in 5% skim milk solution and shaken for 2 h. After blocking, the membrane was placed in the primary antibody solution of GAPDH (1:5000), p-PI3K (1:500), PI3K (1:500), p-Syk (1:500), Syk (1:1000), p-AKT1 (1:500), and AKT1 (1:500) at 4°C overnight.

[0069] Table 3 2.2 Influence of the nasal mucosa related protein expression: the frozen rat nasal mucosa was taken, and the total protein of the nasal mucosa was extracted according to the total protein extraction kit instruction. The loading amount was measured by BCA protein quantification method. The proteins were separated by SDS-PAGE electrophoresis, and the appropriate PVDF membrane was cut and soaked in methanol for 3 min, and then transferred to the PVDF membrane. After the membrane was transferred, the membrane was placed in 5% skim milk solution and shaken for 2 h. After blocking, the membrane was placed in the primary antibody solution of GAPDH (1:5000), p-PI3K (1:500), PI3K (1:500), p-Syk (1:500), Syk (1:1000), p-AKT1 (1:500), and AKT1 (1:500) at 4°C overnight. Figure 1 A: normal control group; B: model control group; C: loratadine 0.9 mg / kg group; D: example 1 group; E: example 2 group; F: example 3 group: From Figure 1It can be seen that the nasal mucosa of the normal control group of rats is complete in structure, the mucosa surface cilia is neat without shedding, the epithelial cells are arranged in order, and the goblet cells exist but there is no obvious proliferation; compared with the normal control group, a large number of goblet cell proliferation and cilia shedding can be seen in the nasal mucosa of the model control group of rats; compared with the model control group, the goblet cell proliferation of the example 1, example 2 and example 3 groups is inhibited, and the number is reduced, among which the improvement of the example 2 and example 3 groups is particularly obvious; the nasal mucosa cilia exists in different degrees of shedding, and the cilia shedding degree of the example 3 group is lower.

[0070] 2.3 Effect of Xuanfei Tongqiao Essential Oil on the Contents of Serum IFN-γ, IL-4, IL-6, IL-17 and TNF-α in AR Rat Model Compared with the normal control group, the content of IFN-γ in the serum of the model control group of rats was significantly reduced, and the contents of IL-4, IL-6, IL-17 and TNF-α were significantly increased (P<0.01); compared with the model control group, the contents of IFN-γ in the serum of the administration groups were significantly increased, and the contents of IL-4, IL-6, IL-17 and TNF-α were significantly reduced (P<0.01), see Table 4 for details. And because the comparative example 1 group did not add coconut oil and cuban balsam essential oil at the same time, the number of nasal cavity bacterial colonization was increased by 102% compared with the example 2 group, the serum TNF-α was increased by 30.6%, IL-6 was increased by 26.2%, and the anti-infection and antibacterial effect was reduced by 50.5%.

[0071] Table 4 2.4 Effect of Xuanfei Tongqiao Essential Oil on the mRNA Expression of Syk, Pik3ca, Pik3cg, Akt1, Prkca, Mapk1, Mapk8, Mapk14, Pla2g4a, Alox5 and Alox5ap in the Nasal Mucosa of AR Rats Compared with the normal control group, the mRNA expression of Syk, Pik3ca, Pik3cg, Akt1, Prkca, Mapk1, Mapk8, Mapk14, Pla2g4a, Alox5 and Alox5ap in the nasal mucosa of the model control group of rats was significantly up-regulated (P<0.01); compared with the model control group, the difference in the mRNA expression of Akt1 and Pla2g4a in the 0.9 mg / kg group of loratadine was not obvious, and the mRNA expression of Syk, Pik3ca, Pik3cg, Akt1, Prkca, Mapk1, Mapk8, Mapk14, Pla2g4a, Alox5 and Alox5ap in the remaining administration groups was significantly down-regulated (P<0.05 or P<0.01). See Tables 5 and 6 for specific results.

[0072] Table 5 Table 6 2.5 The influence of each group of rat model nasal mucosa SYK / PI3K / AKT1 signaling pathway results as shown in Figure 2 Figure 2 A: normal control group; B: loratadine group; C: example 1 group; D: example 2 group; E: example 3 group; the results are shown in Table 7 From Table 7, it can be seen that the model control group p-SYK / SYK, p-PI3K / PI3K, p-AKT1 / AKT1 ratio significantly increased (P<0.01); compared with the model control group, the loratadine 0.9 mg / kg group p-SYK / SYK, p-AKT1 / AKT1 ratio significantly decreased (P<0.01), p-PI3K / PI3K, p-AKT1 / AKT1 ratio significantly decreased (P<0.05 or P<0.01), and the example group p-SYK / SYK, p-PI3K / PI3K, p-AKT1 / AKT1 ratio significantly decreased (P<0.01).

[0073] Table 7 3. Human population experiment Zhejiang Provincial Hospital of Traditional Chinese Medicine Otolaryngology clinic patients from March 2024 to June 2024, observe the efficacy of rhinitis essential oil.

[0074] Set up control group: normal saline (100ml) flush nasal cavity + Nisshin spray, flush twice a day, spray twice a day, a total of 15 cases; Treatment group 1: normal saline (100ml) + example 1 essential oil (0.2ml) flush nasal cavity + Nisshin spray, flush twice a day, spray twice a day, a total of 15 cases, Treatment group 2: normal saline (100ml) + example 2 essential oil (0.2ml) flush nasal cavity + Nisshin spray, flush twice a day, spray twice a day, a total of 15 cases, Treatment group 3: normal saline (100ml) + example 3 essential oil (0.2ml) flush nasal cavity + Nisshin spray, flush twice a day, spray twice a day, a total of 15 cases.

[0075] Treatment group 4: normal saline (100ml) + comparative example 1 essential oil (0.2ml) flush nasal cavity + Nisshin spray, flush twice a day, spray twice a day, a total of 15 cases.

[0076] Treatment group 5: normal saline (100ml) + comparative example 2 essential oil (0.2ml) flush nasal cavity + Nisshin spray, flush twice a day, spray twice a day, a total of 15 cases.

[0077] ​Treatment group 6: physiological saline (100ml) + essential oil of comparative example 3 (0.2ml) to flush the nasal cavity + Nisshuna to spray the nose, flush twice a day, spray twice a day, a total of 15 cases.

[0078] 7 groups of patients were randomly allocated, and the general data were compared, and the differences were not statistically significant (P>0.05), which were comparable.

[0079] Treatment method: the control group was flushed with physiological saline + Nisshuna to spray the nose, flushed twice a day, sprayed twice a day, the average treatment days (7.7±0.3) days, and the treatment group was flushed with physiological saline + essential oil to flush the nasal cavity + Nisshuna to spray the nose, the average treatment days (6.8±0.4) days. The general data of 4 groups of patients were compared, and the differences were not statistically significant (P>0.05), which were comparable.

[0080] Observation index: before and after treatment, the nasal congestion visual analog scale (VAS, Visual Analog Scale) score, FeNO score before and after treatment, and physical sign and symptom score (Lund-kennedy) were used to take the average value. Tables 7-9 are respectively the comparison of visual analog scale (VAS, Visual Analog Scale) score before and after treatment, FeNO score before and after treatment, and physical sign and symptom score (Lund-kennedy) (points, x-±s).

[0081] As can be seen from table 8, the nasal bacterial detection rate of treatment group 4 (comparative example 1) group was increased by 133.5% compared with treatment group 2 (example 2), the serum TNF-α was increased by 30.6%, and the antibacterial effect was reduced by 57.1%. The anti-allergic effect of treatment group 5 (comparative example 2) group was reduced by 81.9%, and the anti-allergic effect of treatment group 6 (comparative example 3) group was reduced by 82.6%.

[0082] Conclusion: before treatment, the symptom and sign scores of 3 groups of patients were compared, and the difference was not statistically significant (P>0.05). After treatment, the symptom and sign scores of three groups of patients were lower than those before treatment, and the treatment group was lower than the control group, and the difference was statistically significant (P<0.001). Conclusion: compared with 3 groups of treatment groups and comparative examples, it can be seen that the use of Nisshuna nasal spray and the nasal congestion essential oil disclosed in the application can greatly improve the efficiency of conventional treatment of nasal congestion.

[0083] Table 8 Table 9 Table 10 The experimental data prove the synergistic effect between the essential oils: 1. Synergistic anti-infective effect of cubeb and coconut oil lauric acid (animal + human experiment verification) Animal experiments show that the number of bacterial colonization in the nasal cavity doubles after the lack of cubeb, and the serum inflammatory factors increase significantly. Human experiments show that the bacterial detection rate increases by 133.5% after the lack of cubeb, which confirms the synergy between the two: coconut oil lauric acid enhances the anti-inflammatory activity of cubeb caryophyllene, and cubeb broadens the antibacterial spectrum of lauric acid. The anti-infective effect of either component is halved, and the synergy is inseparable.

[0084] 2. Synergistic anti-allergic effect of orange flower and bitter orange (animal + human experiment verification) In animal experiments, the lack of only orange flower / bitter orange increases the histamine release rate by 180%+, and the mast cell degranulation rate by 180%+. In human experiments, the nasal itching VAS score increases by 160%+, and the histamine concentration increases by 140%+, which confirms the synergy between the two: orange flower linalool regulates nerve sensitivity, and bitter orange hesperidin inhibits histamine release. The double path cannot be missing, and the anti-allergic effect of either component is reduced by more than 80%.

[0085] 4. Specific cases Mr. Wang, male, 38 years old, from Hangzhou, Zhejiang Province. He came to the hospital in April 2024. His nasal congestion, itching, and sneezing had worsened for more than ten days. After using the essential oil described in the invention to flush the nasal cavity (Example 1) for 5 days, the above symptoms were significantly relieved. Medical Case 2: Mr. Chen, male, 27 years old, from Hangzhou, Zhejiang Province. He came to the hospital in May 2024. He had recurrent nasal congestion for three months, which was sometimes mild and sometimes severe. After using the essential oil described in the invention to flush the nasal cavity (Example 1) for 7 days, the symptoms were significantly relieved.

[0086] Medical Case 3: Ms. Su, female, 35 years old, from Hangzhou, Zhejiang Province. She came to the hospital in March 2024. She had symptoms of nasal congestion and decreased olfaction after COVID-19 infection, which were sometimes mild and sometimes severe, and were accompanied by sleep disorders. After using the essential oil of the invention (Example 3) for 10 days, the symptoms were significantly relieved.

[0087] From the above embodiments, it can be seen that the essential oil composition can relieve mental anxiety and tension caused by nasal symptoms (nasal congestion, runny nose, sneezing, nasal itching, decreased olfaction, dizziness and head swelling, etc.), sleep disorders, can assist in treating nasal congestion, runny nose, sneezing, nasal itching, decreased olfaction, etc., especially acute and chronic rhinitis, allergic rhinitis, has the advantages of fast effect, no trauma, low price, no negative effect, simple operation, etc., is suitable for application in daily nursing and treatment of patients with rhinitis. The above is only a limited number of preferred embodiments of the present application, which is described in more detail and in detail, but it cannot be understood as limiting the scope of the patent of the present application. It should be pointed out that for ordinary skilled persons in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the scope of protection of the present application.

Claims

1. A passageway unblocking lung essence oil, characterized in that, The essential oil composition is prepared by mixing the following essential oils: 40-70 parts of sweet almond oil, 10-30 parts of refined jojoba oil, 2-10 parts of cuban balsam essential oil, 1-5 parts of orange flower essential oil, 1-5 parts of sweet bay essential oil, 1-5 parts of black leaf mint essential oil, 1-5 parts of eucalyptus essential oil, 1-5 parts of fir essential oil, 1-3 parts of bitter orange essential oil, 1-4 parts of magnolia essential oil, and 1-4 parts of perilla essential oil.

2. The method of claim 1, wherein the preparation of the eye and lung clearing essential oil is characterized by, The method comprises the following steps: a. equal amounts of cuban balsam, orange flower, sweet bay, black leaf mint, eucalyptus, fir, magnolia bud, and perilla stem and leaf are cleaned and crushed to obtain corresponding plant solid fragments, and the plant solid fragments are extracted by steam distillation to obtain cuban balsam essential oil, orange flower essential oil, sweet bay essential oil, black leaf mint essential oil, eucalyptus essential oil, fir essential oil, magnolia essential oil, and perilla essential oil, respectively; b. equal amounts of bitter orange peels as in step a) are cleaned, and extracted by cold pressing to obtain bitter orange essential oil, wherein the temperature during the extraction is controlled at 5-10℃; c. equal amounts of sweet almond as in step a) are shelled, cleaned, and crushed into a paste, and then extracted by heating at a temperature of 60-80℃ to obtain oil, and then the extracted product is filtered and the filtrate is collected to obtain sweet almond oil, wherein the vacuum distillation temperature is 240-280℃; d. equal amounts of jojoba seeds as in step a) are cleaned, and extracted by cold pressing to obtain refined jojoba oil; e. the cuban balsam essential oil, orange flower essential oil, sweet bay essential oil, black leaf mint essential oil, eucalyptus essential oil, fir essential oil, bitter orange essential oil, sweet almond oil, magnolia essential oil, perilla essential oil, and refined jojoba essential oil obtained in the above steps are mixed to obtain an essential oil composition.

3. The production method according to claim 2, characterized by, In step a), when the plant essential oils are extracted by steam distillation, the mass ratio of water to plant solid fragments is 4-6:

1.

4. The production method according to claim 2, wherein In step d), the temperature during the cold pressing is 40-60℃, the cold pressing pressure is 20-30MPa, and the extraction time is 30-60 minutes.

5. The production method according to claim 2, characterized by, In step d), the number of fractionation trays used during the fractionation is 25-30, and the reflux index is 2-9.

6. The production method according to claim 2, wherein The cuban balsam, orange flower, sweet bay, black leaf mint, eucalyptus, fir, bitter orange, sweet almond, jojoba, magnolia, and perilla are all organic crops.

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