Analysis kit and detection method of prostate small body leaked protein
By introducing an electric heating element and a microcontroller into the prostate small effusive protein detection kit, the problems of lack of temperature balance and time control in the kit are solved, ensuring the accuracy of the test results and avoiding errors caused by temperature fluctuations and reagent failure.
Patent Information
- Application Number
- CN202511165122.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-20
- Publication Date
- 2025-11-21
AI Technical Summary
Existing small prostate protein leak detection kits lack temperature balance control mechanisms and reagent time-sensitivity control functions, resulting in inaccurate test results and affecting the clinical diagnosis and disease assessment of prostate diseases.
A reagent kit with an electric heating element, a temperature sensor, and a microcontroller was designed. The temperature sensor detects temperature changes and activates the room temperature equilibration timing module and the failure judgment timing module to ensure that the reagent is used in time after equilibration at room temperature, and to forcibly discard the failed reagent after the timeout to prevent detection errors.
This system enables the reagents to be forced to wait until they reach room temperature equilibrium and to be forced to be discarded after they become ineffective, ensuring the accuracy of the test results, avoiding false positives or false negatives, and improving the reliability of the test.
Smart Images
Figure CN120986845A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of reagent kit technology, and in particular to an analytical kit and detection method for prostatic small effusive proteins. Background Technology
[0002] In the clinical diagnosis of prostate diseases (such as prostatitis and benign prostatic hyperplasia), prostatic small exudate proteins serve as key biomarkers. The accuracy of their detection results directly affects the reliability of disease diagnosis and condition assessment. Sample and reagent management before testing is the core link that determines the accuracy of the results. Currently, the reagent kits used for routine prostate small effusive protein testing generally suffer from the following technical defects: Lack of temperature equilibration control mechanism: Testing supplies (reagents, test strips, sample processing solutions, etc.) must strictly adhere to the 2-8℃ refrigerated storage standard. After being removed from the refrigerated environment, they need to be equilibrated at room temperature for about 20 minutes to ensure reagent activity stability and sample reaction consistency. However, existing kits lack mandatory temperature control and time-locking functions. Operators are prone to negligence (such as rushing to test) and prematurely using the kits before the equilibration time is reached, leading to sudden temperature changes that alter reagent activity and decrease sample stability, directly causing false positives or false negatives in the test results. Lack of reagent timeliness control: Testing supplies must be used promptly after room temperature equilibration. Beyond the time limit, their activity will significantly decrease with prolonged storage, and stability will be greatly reduced. However, existing kits cannot automatically monitor and intervene in reagent timeliness after room temperature exposure. If operators do not use the kits in time, expired reagents may still be misused for testing, leading to inaccurate diagnostic results and affecting the formulation of clinical treatment plans. Summary of the Invention
[0003] This invention relates to an analytical kit for small prostatic effusive proteins and its detection method, which solves the problem that current small prostatic effusive protein detection kits lack temperature balance control mechanisms and reagent time-effect control functions, thus affecting the accuracy of detection results and interfering with the clinical diagnosis and disease assessment of prostate diseases.
[0004] This invention provides an analytical kit for small prostatic effusive proteins, specifically comprising: a box body, wherein a storage slot is formed on the top surface of the box body, a heating chamber is formed inside the box body adjacent to the lower part of the storage slot, an electric heating element is fixedly installed on the top surface of the heating chamber, and a row of heat-conducting holes connected to the heating chamber are formed on the front and rear surfaces of the box body corresponding to the heating chamber; a box cover is rotatably installed on the rear edge of the top surface of the box body, a microcontroller is installed inside the box cover, and the microcontroller is electrically connected to the electric heating element; a temperature controller electrically connected to the microcontroller is installed on the bottom surface of the box cover; and a device is embedded in the top surface of the box cover. The device includes a temperature sensor electrically connected to a microcontroller; a storage groove with an arc-shaped structure is provided on the front side of the bottom end of the lid; a drive mounting groove is provided on the rear side of the bottom end of the lid adjacent to the storage groove, and a micro motor is fixedly mounted on the front side of the drive mounting groove, the micro motor being electrically connected to the microcontroller; a circular rotating block is rotatably mounted inside the storage groove, the diameter of the circular rotating block being the same as the diameter of the storage groove, and the thickness of the circular rotating block being the same as the width of the storage groove; the shaft end of the micro motor passes through the storage groove and is fixedly connected to the central part of the circular rotating block.
[0005] Furthermore, a limiting slot is provided on the front side of the top surface of the box body. The limiting slot has an arc-shaped groove structure, and the diameter of the limiting slot is consistent with that of the storage groove. The width of the limiting slot is also consistent with that of the storage groove. When the bottom surface of the box lid is in contact with the top surface of the box body, the storage groove and the limiting slot are in corresponding positions, and together they form a complete circular structure.
[0006] Furthermore, the outer circumferential surface of the circular rotating block has a cross section. When the cross section faces downward, the circular rotating block is completely housed inside the storage groove. When the bottom surface of the box lid is in contact with the top surface of the box body and the cross section is not facing downward, the circular rotating block is rotatably inserted into the limiting slot.
[0007] Furthermore, an opening / closing mounting groove is provided on the front side of the bottom end of the lid, and an opening / closing switch is installed inside the opening / closing mounting groove. The opening / closing switch is electrically connected to the microcontroller. The opening / closing switch is a tactile switch, and the button end of the opening / closing switch faces downward. When the opening / closing switch is in the unpressed state, the button end of the opening / closing switch is located outside the opening / closing mounting groove. When the bottom end of the lid is in contact with the top end of the box body, the button end of the opening / closing switch is in contact with the top end of the box body, and at this time the opening / closing switch is in the pressed-on state.
[0008] Furthermore, the top surface of the box body has two symmetrically arranged latching slots; the bottom surface of the box cover has two symmetrically arranged latching pieces installed. When the latching pieces are engaged with the latching slots, the bottom surface of the box cover is in close contact with the top surface of the box body; a battery is installed inside the box cover, and a charging port electrically connected to the battery is installed on the left side of the box cover.
[0009] Furthermore, a flashing indicator light and a display screen are installed on the top surface of the box cover. Both the flashing indicator light and the display screen are electrically connected to the microcontroller. When the on / off switch is in the pressed start state, the on / off switch feeds a feedback signal to the microcontroller, which controls the shaft end of the micro motor to rotate 180 degrees, at which time the cross-section is facing upwards.
[0010] Furthermore, the inside of the box cover is equipped with a room temperature balance timing module electrically connected to the microcontroller; the timing value of the room temperature balance timing module is 20 minutes; when the temperature sensor detects that the temperature is higher than the threshold, the temperature sensor sends a feedback signal to the microcontroller, and the microcontroller controls the room temperature balance timing module to start; when the timing value of the room temperature balance timing module is reached, the room temperature balance timing module sends a feedback signal to the microcontroller, and the microcontroller controls the shaft end of the micro motor to rotate 180 degrees, at which time the cross section is facing downwards, and at the same time controls the flashing indicator light to start.
[0011] Furthermore, the inside of the box cover is also equipped with a failure judgment timing module and a discard unlocking timing module electrically connected to the microcontroller; the timing value of the failure judgment timing module is one hour; the timing value of the discard unlocking timing module is ten minutes; when the timing value of the room temperature equilibrium timing module is reached, the microcontroller also controls the failure judgment timing module to start; when the timing value of the failure judgment timing module is reached, the failure judgment timing module sends a feedback signal to the microcontroller, and the microcontroller controls the shaft end of the micro motor to rotate 180 degrees, at which time the cross-section is facing upwards; the microcontroller also controls the electric heating element and the discard unlocking timing module to start; when the timing value of the discard unlocking timing module is reached, the discard unlocking timing module sends a feedback signal to the microcontroller, and the microcontroller controls the electric heating element to turn off and controls the shaft end of the micro motor to rotate 180 degrees, at which time the cross-section is facing downwards.
[0012] This invention provides an analytical kit and detection method for prostatic small effusive proteins, which have the following beneficial effects: In this invention's reagent kit, when the temperature sensor detects a temperature higher than a threshold, it triggers a room temperature equilibration timing module (20 minutes). During the timing period, a micro motor drives a circular rotating block to insert into the box's limiting slot and lock the lid, forcibly preventing the test items from being used before equilibration to room temperature, thus avoiding changes in reagent activity and decreased sample stability due to temperature fluctuations. After the room temperature equilibration timing ends, the microcontroller simultaneously starts a failure judgment timing module (1 hour). If the test items are not used within 1 hour, the module sends a feedback signal to the microcontroller to control the micro motor to lock the lid again and activate the electric heating element in the box's heating chamber to forcibly discard the reagents, preventing inactive reagents from causing detection errors. This dual approach of pre-detection preparation and reagent timeliness control ensures accurate detection. Attached Figure Description
[0013] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings of the embodiments will be briefly described below.
[0014] The accompanying drawings described below are only related to some embodiments of the invention and are not intended to limit the invention.
[0015] In the attached diagram: Figure 1 A schematic diagram of the front isometric structure of the present invention is shown; Figure 2 A schematic diagram of the rear isometric structure of the present invention is shown; Figure 3 A schematic diagram of the structure of the lid of the present invention in the rotated and unfolded state is shown; Figure 4 The present invention is shown Figure 3 A magnified view of the structure at point A in the middle; Figure 5 The present invention is shown Figure 4 Schematic diagram of the micro-motor and circular rotating block in disassembled state; Figure 6 A cross-sectional structural schematic diagram of the present invention is shown; Figure 7 The present invention is shown Figure 6 Schematic diagram of the cross-sectional structure of the middle BB; Figure 8 A system composition block diagram of the present invention is shown; List of reference numerals 1. Box body; 101. Heat conduction hole; 102. Storage slot; 103. Snap-on slot; 104. Limiting slot; 105. Heating chamber; 106. Electric heating element; 2. Box cover; 201. Snap-on component; 202. Flashing indicator light; 203. Display screen; 204. Temperature sensor; 205. Charging port; 206. Thermostat; 207. Micro motor; 208. Drive mounting slot; 209. Circular rotating block; 2010. Opening and closing mounting slot; 2011. Opening and closing switch; 2012. Storage slot; 2013. Cross section; 2014. Battery; 2015. Room temperature balance timing module; 2016. Failure judgment timing module; 2017. Discard unlocking timing module; 2018. Microcontroller. Detailed Implementation
[0016] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the described embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0017] Example: Please refer to Figures 1 to 8 : This invention proposes an analytical kit and detection method for prostatic small effusive proteins, comprising: a box body 1, with a storage slot 102 on the top surface of the box body 1, a heating chamber 105 located inside the box body 1 adjacent to the lower part of the storage slot 102, an electric heating element 106 fixedly installed on the top surface of the inner end of the heating chamber 105, and a row of heat-conducting holes 101 connected to the heating chamber 105 on the front and rear surfaces of the box body 1; a box cover 2 rotatably mounted on the rear edge of the top surface of the box body 1, with a microcontroller 2018 inside the box cover 2, electrically connected to the electric heating element 106; a temperature controller 206 electrically connected to the bottom surface of the box cover 2; a temperature sensor 204 embedded in the top surface of the box cover 2, electrically connected to the microcontroller 2018; a receiving slot 2012 with an arc-shaped groove structure located on the front side of the bottom surface of the box cover 2; and a receiving slot 2012 located on the bottom surface of the box cover 2 adjacent to the receiving slot 2018. A drive mounting slot 208 is provided on the rear side of component 12. A micro motor 207 is fixedly mounted on the front side of the inner end of the drive mounting slot 208. The micro motor 207 is electrically connected to the microcontroller 2018. A circular rotating block 209 is rotatably mounted inside the storage slot 2012. The diameter of the circular rotating block 209 is the same as the diameter of the storage slot 2012, and the thickness of the circular rotating block 209 is also the same as the width of the storage slot 2012. The shaft end of the micro motor 207 passes through the storage slot 2012. It is fixedly connected to the central part of the circular rotating block 209; a limiting slot 104 is opened on the front side of the top face of the box body 1. The limiting slot 104 has an arc-shaped groove structure. The diameter of the limiting slot 104 is the same as that of the storage groove 2012, and the width of the limiting slot 104 is also the same as that of the storage groove 2012; when the bottom face of the box cover 2 is in contact with the top face of the box body 1, the storage groove 2012 and the limiting slot 104 are in corresponding positions, and together they form a complete circular structure.
[0018] The circular rotating block 209 has a cross-section 2013 on its outer circumference. When the cross-section 2013 faces downwards, the circular rotating block 209 is completely housed inside the storage groove 2012. When the bottom surface of the lid 2 is in contact with the top surface of the box body 1, and the cross-section 2013 is not facing downwards, the circular rotating block 209 is rotatably inserted into the limiting slot 104. An opening / closing mounting groove 2010 is provided on the front side of the bottom surface of the lid 2, and an opening / closing switch 2011 is installed inside the opening / closing mounting groove 2010. The closed switch 2011 is electrically connected to the microcontroller 2018; the open / close switch 2011 is a tactile switch, with its button facing downwards; when the open / close switch 2011 is in the unpressed state, its button is located outside the opening / closing mounting slot 2010; when the bottom surface of the cover 2 is in contact with the top surface of the body 1, the button of the open / close switch 2011 is in contact with the top surface of the body 1, at which point the open / close switch 2011 is in the pressed / activated state; the top surface of the body 1 is... The cover 2 has two symmetrically arranged latching slots 103; the bottom surface of the cover 2 is also symmetrically equipped with two latching pieces 201. When the latching pieces 201 are engaged with the latching slots 103, the bottom surface of the cover 2 is in close contact with the top surface of the box body 1; the cover 2 contains a battery 2014, and the left end of the cover 2 has a charging port 205 electrically connected to the battery 2014; the top surface of the cover 2 has a flashing indicator light 202 and a display screen 203, which displays the remaining power of the battery 2014. The countdown values for starting the timing of the quantity and room temperature balance timing module 2015, the failure judgment timing module 2016, and the discard unlocking timing module 2017, the flashing indicator light 202, and the display screen 203 are all electrically connected to the microcontroller 2018. When the on / off switch 2011 is in the pressed start state, the on / off switch 2011 sends a feedback signal to the microcontroller 2018, and the microcontroller 2018 controls the shaft end of the micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing upwards.
[0019] The box cover 2 contains a room temperature balance timing module 2015 electrically connected to a microcontroller 2018. The timing value of the room temperature balance timing module 2015 is 20 minutes. When the temperature sensor 204 detects a temperature higher than a threshold, the temperature sensor 204 sends a feedback signal to the microcontroller 2018, which then controls the room temperature balance timing module 2015 to start. When the timing value of the room temperature balance timing module 2015 is reached, the room temperature balance timing module 2015 sends a feedback signal to the microcontroller 2018, which then controls the shaft of the micro motor 207 to rotate 180 degrees, at which point the cross-section 2013 faces downwards, and simultaneously controls the flashing indicator light 202 to start. The box cover 2 also contains a failure judgment timing module 2016 and a discard unlocking timing module 2017 electrically connected to the microcontroller 2018. The timing value of the failure judgment timing module 2016 is one hour. The timing value of the discard unlocking timing module 2017 is ten minutes. When the timing value of the room temperature equilibrium timing module 2015 is reached, the microcontroller 2018 also controls the failure judgment timing module 2016 to start. When the timing value of the failure judgment timing module 2016 is reached, the failure judgment timing module 2016 sends a feedback signal to the microcontroller 2018, and the microcontroller 2018 controls the shaft end of the micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing upward. The microcontroller 2018 also controls the electric heating element 106 and the discard unlocking timing module 2017 to start. When the timing value of the discard unlocking timing module 2017 is reached, the discard unlocking timing module 2017 sends a feedback signal to the microcontroller 2018, and the microcontroller 2018 controls the electric heating element 106 to turn off and controls the shaft end of the micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing downward.
[0020] The working principle of this embodiment: Test strips, reagents, sample processing solutions, sample collection tubes, and other testing supplies used for the analysis and detection of prostatic small effusive proteins are all stored in storage tank 102, and must be refrigerated in strict accordance with storage requirements before use. During refrigeration, the position of the lid 2 is temporarily fixed by the fastener 201 engaging with the fastener slot 103. At this time, the button end of the on / off switch 2011 is in contact with the top surface of the box body 1, and the on / off switch 2011 is in the pressed-on state. The on / off switch 2011 sends a feedback signal to the microcontroller 2018, and the microcontroller 2018 controls the shaft end of the micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing upwards, and part of the structure of the circular rotating block 209 is inserted into the limiting slot 104 to limit and fix the lid 2 in the closed state. After the box 1 is taken out of the refrigerated environment, it must be equilibrated to room temperature (usually 20 minutes) before it can be used to ensure accurate test results. At this time, because part of the structure of the circular rotating block 209 is inserted into the limiting slot 104, the lid 2 in the closed state is limited and fixed. Therefore, after the box 1 is taken out of the refrigerated environment, the operator cannot rotate and open the lid 2, so that it cannot come into contact with the test items stored in the storage compartment 102, thereby avoiding the operator from conducting the test application before equilibrating to room temperature as required. When box 1 is removed from the refrigerated environment, temperature sensor 204 detects that the temperature is higher than the threshold and sends a feedback signal to microcontroller 2018. Microcontroller 2018 controls room temperature balance timing module 2015 to start. The timing value of room temperature balance timing module 2015 is 20 minutes. When the timing value of room temperature balance timing module 2015 is reached, room temperature balance timing module 2015 sends a feedback signal to microcontroller 2018. Microcontroller 2018 controls the shaft end of micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing downwards, and part of the structure of circular rotating block 209 is disengaged from the limiting slot 104, releasing the limiting fixation of the closed box cover 2. At the same time, it controls the flashing indicator light 202 to start. Based on the unlocking of the limiting fixation and the flashing of the flashing indicator light 202, it can indicate to the operator that the box cover 2 can be rotated and opened to take out the testing items in storage slot 102 for corresponding testing operations. Furthermore, when the timing value of the room temperature balancing timing module 2015 is reached, the microcontroller 2018 also controls the failure judgment timing module 2016 to start. The timing value of the failure judgment timing module 2016 is one hour (i.e., the test item has not been touched or used by the operator for one hour after balancing to room temperature). When the timing value of the failure judgment timing module 2016 is reached, the failure judgment timing module 2016 sends a feedback signal to the microcontroller 2018, and the microcontroller 2018 controls the shaft end of the micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing upwards. At this time, due to the circular rotating block 209... Part of the structure is repositioned and inserted into the limiting slot 104 to limit and fix the lid 2 in the closed state, preventing operators from taking out reagents and other testing supplies that are likely to have changed activity or decreased stability, affecting the accuracy of the test. At the same time, the microcontroller 2018 also controls the electric heating element 106 and the waste unlocking timer module 2017 to start. First, the electric heating element 106 starts heating, and the heat generated is transferred into the storage tank 102. The reagents in the storage tank 102 that have been left at room temperature for too long are likely to have changed activity or decreased stability, affecting the accuracy of the test, and are subjected to heating treatment to achieve forced waste disposal. When the timer value (ten minutes) of the waste unlocking timer module 2017 is reached, the waste unlocking timer module 2017 sends a feedback signal to the microcontroller 2018. The microcontroller 2018 controls the electric heating element 106 to turn off and controls the shaft end of the micro motor 207 to rotate 180 degrees. At this time, the cross section 2013 is facing downwards, thereby releasing the limiting fixation of the closed box cover 2 again, so that the operator can rotate and open the box cover 2, take out the testing items for forced disposal in the storage slot 102, and complete the disposal in accordance with safety, compliance and environmental protection requirements.
Claims
1. A kit for analyzing prostatic small effusive proteins, characterized in that, include: The box body (1) has a storage slot (102) on its top surface. A heating chamber (105) is provided inside the box body (1) adjacent to the storage slot (102). An electric heating element (106) is fixedly installed on the top surface of the heating chamber (105). A row of heat-conducting holes (101) connected to the heating chamber (105) is provided on the front and rear surfaces of the box body (1). A box cover (2) is rotatably installed on the rear edge of the top surface of the box body (1). A microcontroller (2018) is provided inside the box cover (2). The microcontroller (2018) is electrically connected to the electric heating element (106). A temperature controller (206) is installed on the bottom surface of the box cover (2). A temperature sensor (204) is embedded in the top surface of the box cover (2). The temperature sensor (204) is connected to the microcontroller (2018). 018) Electrically connected; a storage groove (2012) is provided on the front side of the bottom end of the box cover (2), and the storage groove (2012) has an arc-shaped groove structure; a drive mounting groove (208) is provided on the rear side of the bottom end of the box cover (2) adjacent to the storage groove (2012), and a micro motor (207) is fixedly installed on the front side of the inner end of the drive mounting groove (208), and the micro motor (207) is electrically connected to the microcontroller (2018); a circular rotating block (209) is rotatably installed inside the storage groove (2012), the diameter of the circular rotating block (209) is consistent with the diameter of the storage groove (2012), and the thickness of the circular rotating block (209) is also consistent with the width of the storage groove (2012); the shaft end of the micro motor (207) passes through the storage groove (2012) and is fixedly connected to the shaft center of the circular rotating block (209).
2. The analytical kit for small prostatic effusion proteins according to claim 1, characterized in that, A limiting slot (104) is provided on the front side of the top surface of the box body (1). The limiting slot (104) has an arc-shaped groove structure. The diameter of the limiting slot (104) is the same as that of the storage groove (2012), and the width of the limiting slot (104) is also the same as that of the storage groove (2012). When the bottom surface of the box cover (2) is in contact with the top surface of the box body (1), the storage groove (2012) and the limiting slot (104) are in corresponding positions, and together they form a complete circular structure.
3. The analytical kit for small prostatic effusion proteins according to claim 2, characterized in that, The outer circumferential surface of the circular rotating block (209) has a cross section (2013). When the cross section (2013) is facing downward, the circular rotating block (209) is completely stored inside the storage groove (2012). When the bottom surface of the box cover (2) is in contact with the top surface of the box body (1) and the cross section (2013) is not facing downward, the structure of the circular rotating block (209) is rotated and inserted into the limiting slot (104).
4. The analytical kit for small prostatic effusion proteins according to claim 3, characterized in that, The bottom end of the cover (2) is provided with an opening and closing mounting groove (2010) on the front side. An opening and closing switch (2011) is installed inside the opening and closing mounting groove (2010). The opening and closing switch (2011) is electrically connected to the microcontroller (2018). The opening and closing switch (2011) is a tactile switch, and the button end of the opening and closing switch (2011) faces downward. When the opening and closing switch (2011) is in the unpressed start state, the button end of the opening and closing switch (2011) is located outside the opening and closing mounting groove (2010). When the bottom end of the cover (2) is in contact with the top end of the box body (1), the button end of the opening and closing switch (2011) is in contact with the top end of the box body (1). At this time, the opening and closing switch (2011) is in the pressed start state.
5. The analytical kit for small prostatic effusion proteins according to claim 4, characterized in that, The top surface of the box body (1) is symmetrically provided with two buckle slots (103); the bottom surface of the box cover (2) is symmetrically provided with two buckle pieces (201). When the buckle piece (201) is engaged with the buckle slot (103), the bottom surface of the box cover (2) is in close contact with the top surface of the box body (1); the box cover (2) is provided with a storage battery (2014), and the left end of the box cover (2) is provided with a charging port (205) that is electrically connected to the storage battery (2014).
6. The analytical kit for small prostatic effusion proteins according to claim 5, characterized in that, The top surface of the cover (2) is equipped with a flashing indicator light (202) and a display screen (203). Both the flashing indicator light (202) and the display screen (203) are electrically connected to the microcontroller (2018). When the on / off switch (2011) is in the pressed start state, the on / off switch (2011) sends a feedback signal to the microcontroller (2018), and the microcontroller (2018) controls the shaft end of the micro motor (207) to rotate 180 degrees. At this time, the cross section (2013) is facing upward.
7. The analytical kit for small prostatic effusion proteins according to claim 6, characterized in that, The box cover (2) is equipped with a room temperature balance timing module (2015) electrically connected to the microcontroller (2018); the timing value of the room temperature balance timing module (2015) is twenty minutes; when the temperature sensor (204) senses that the temperature is higher than the threshold, the temperature sensor (204) sends a feedback signal to the microcontroller (2018), and the microcontroller (2018) controls the room temperature balance timing module (2015) to start; when the timing value of the room temperature balance timing module (2015) is reached, the room temperature balance timing module (2015) sends a feedback signal to the microcontroller (2018), and the microcontroller (2018) controls the shaft end of the micro motor (207) to rotate 180 degrees, at which time the cross section (2013) is facing downwards, and at the same time controls the flashing indicator light (202) to start.
8. The analytical kit for small prostatic effusion proteins according to claim 7, characterized in that, The box cover (2) is also equipped with a failure judgment timing module (2016) and a discard unlocking timing module (2017) electrically connected to the microcontroller (2018); the timing value of the failure judgment timing module (2016) is one hour; the timing value of the discard unlocking timing module (2017) is ten minutes; when the timing value of the room temperature equilibrium timing module (2015) is reached, the microcontroller (2018) also controls the failure judgment timing module (2016) to start, and when the timing value of the failure judgment timing module (2016) is reached, the failure judgment timing module (2016) sends a feedback signal to the microcontroller (2018). The microcontroller (2018) controls the shaft end of the micro motor (207) to rotate 180 degrees. At this time, the cross section (2013) is facing upward. The microcontroller (2018) also controls the electric heating element (106) and the waste unlocking timer module (2017) to start. When the time value of the waste unlocking timer module (2017) is reached, the waste unlocking timer module (2017) sends a feedback signal to the microcontroller (2018). The microcontroller (2018) controls the electric heating element (106) to turn off and controls the shaft end of the micro motor (207) to rotate 180 degrees. At this time, the cross section (2013) is facing downward.
9. The method for detecting prostatic small effusive proteins as described in any one of claims 1-8, characterized in that, Includes the following steps:
1. Place all the necessary supplies for the detection of prostate small effusive protein, such as test strips, reagents, sample processing solution, and special sample collection tubes, in the storage slot (102) of the box (1). Before use, strictly follow the 2-8℃ cold storage standard to ensure the activity and stability of the supplies. When refrigerated, the buckle (201) of the box cover (2) is fastened to the buckle slot (103) of the box body (1), triggering the on / off switch (2011). The on / off switch (2011) transmits the signal to the microcontroller (2018), which controls the micro motor (207) shaft to rotate 180°, so that the circular rotating block (209) part of the structure is inserted into the limiting slot (104), realizing the double locking of the box cover (2) in the closed state.
2. After the test box is taken out of the refrigerated environment, the temperature sensor (204) monitors the ambient temperature in real time. When the sensed temperature is higher than the refrigeration threshold (usually >8℃), it immediately sends a signal to the microcontroller (2018) and automatically starts the room temperature equilibration timing module (2015). During the equilibration period, the box lid (2) remains locked to prevent the operator from taking the test box before the equilibration time is reached, which may cause the reagent to affect the accuracy of the test due to the sudden temperature change.
3. When the room temperature equilibration timer reaches 20 minutes, the room temperature equilibration timer module (2015) sends a completion signal to the microcontroller (2018). The microcontroller (2018) immediately controls the micro motor (207) to rotate 180° in the opposite direction, so that the circular rotating block (209) is disengaged from the limit slot (104), the box cover (2) is unlocked, and the flashing indicator light (202) is activated to clearly indicate to the operator that the box cover (2) can be opened. At this time, the box cover (2) is opened, and the complete set of testing supplies is taken out from the storage slot (102). The integrity and expiration date of the packaging of the supplies are checked. After confirming that there is no damage or deterioration, they are ready for use.
4. Use a dedicated sample collection tube to collect urine samples from the subject according to the standard procedure (usually collect 5-10ml of midstream urine to avoid sample contamination). Take 1 ml of the processed urine sample and add it to the sample processing solution tube. Gently invert and mix 3-5 times. Let it stand for 2 minutes to allow the prostatic effusive proteins in the sample to be fully released and react with the processing solution. Remove the test strip and vertically immerse the sample application end of the test strip into the treated sample solution (the liquid level should not exceed the MAX line on the test strip). After 10-15 seconds, remove the strip and place it flat on a clean disposable tray. Wait 10-15 minutes for the reaction time and observe the control line (C line) and test line (T line) on the test strip: if both the C line and the T line show color, the result is positive; if only the C line shows color, the result is negative; if the C line does not show color, the test is invalid and a new sample needs to be taken for testing.
5. If the test item is not used within 1 hour after the room temperature is balanced (the timing cycle of the failure judgment timing module (2016)), the failure judgment timing module (2016) sends a timeout signal to the microcontroller (2018). The microcontroller (2018) controls the micro motor (207) to rotate 180° again and lock the box cover (2) again. At the same time, the electric heating element (106) is activated to raise the temperature in the storage tank (102) to 50-60° through conduction heating for 10 minutes. The items that have expired (change in activity, decrease in stability) are forcibly discarded to destroy the activity of the reagent and avoid misuse for testing.
6. When the waste unlocking timer module (2017) reaches 10 minutes, the waste unlocking timer module (2017) sends a signal to the microcontroller (2018) to immediately turn off the electric heating element (106) and at the same time instruct the micro motor (207) to rotate in the opposite direction to unlock the box cover (2). After the operator opens the box cover (2), he / she uses disposable gloves to take out the discarded testing supplies (including remaining reagents, used / unused test strips, and sample tubes) in the storage tank (102), sorts them and puts them into the special packaging bag for medical waste, and hands them over to a qualified medical waste disposal agency for unified disposal in accordance with the requirements of the "Regulations on the Management of Medical Waste".