Preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan

Abstract

The invention belongs to the technical field of saccharomyces cerevisiae beta-glucan, and relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan. The invention relates to a preparation method of saccharomyces cerevisiae-sourced yeast beta-glucan, which comprises the following steps: uniformly mixing a yeast raw material with a first solvent, and carrying out high-pressure homogenization to obtain a homogenized feed liquid; adding food-grade alkali into the homogenized feed liquid to carry out extraction reaction, centrifuging the obtained reaction extracting solution, and separating a heavy phase to obtain a heavy-phase insoluble cell wall extract; and continuously adding a second solvent for resuspension, adding a compound enzyme preparation, adjusting the pH value of the reaction system by using an acidic reagent, carrying out an enzymatic hydrolysis reaction, centrifuging after the reaction is finished, recovering a heavy phase, and drying to obtain the insoluble yeast beta-glucan dry powder. According to the preparation method of the yeast glucan, provided by the invention, the conventional preparation process flow is shortened, the environmental protection treatment pressure is greatly relieved, the production cost is effectively reduced, and the prepared yeast glucan is higher in purity and has a better industrial application prospect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of Saccharomyces cerevisiae β-glucan and relates to a preparation method of Saccharomyces cerevisiae-derived yeast β-glucan. BACKGROUND

[0002] Yeast β-glucan, also known as yeast glucan or dextran, is a natural microbial polysaccharide. The molecular weight of yeast β-glucan is 20-4000 kDa, which is a glucose polymer. The basic structure is a chain with a β-1, 3-glucoside bond as the main chain and a β-1, 6-glucoside bond as the branch chain. The finished product is a light yellow to yellow-brown powder with a special odor and taste.

[0003] In the 1940s, the immune-enhancing effect of yeast β-glucan was discovered, and then it was gradually studied as a health product ingredient. It is currently believed that yeast β-glucan can activate immune cells and regulate the immune function of the intestinal tract and upper respiratory tract to resist bacterial infection and viral invasion.

[0004] Currently, the main methods for extracting yeast β-glucan from yeast are acid-base treatment, enzyme treatment and organic reagent treatment. Chinese patent CN116554362A discloses a preparation process of yeast glucan, which uses ordinary yeast cell walls as raw materials and calcium oxide as an alkali treatment material. After being treated with alkaline protease and acetone solution, yeast β-glucan with a purity of more than 70% is obtained. Chinese patent CN118909156A discloses a preparation process of yeast glucan, in which organic solvents and alkali reagents are used to remove impurities in the yeast raw materials, and then acid extraction is performed to obtain yeast β-glucan with a purity of 82%-91%. Although the above-mentioned existing methods can obtain yeast β-glucan with high purity, they all use organic reagents, which have low production safety and high requirements for equipment and control, and have certain implementation risks in industry. SUMMARY

[0005] The technical problem to be solved by the present application is that yeast β-glucan needs to be extracted with organic reagents in the prior art, which has low production safety and high requirements for equipment and control. The present application provides a preparation method of Saccharomyces cerevisiae-derived yeast β-glucan, which does not need to use organic reagents, has high production safety, relatively low requirements for equipment and control, and can obtain yeast β-glucan with high purity under relatively simple operation.

[0006] To solve the above technical problems, the technical scheme adopted by the present application is as follows:

[0007] The present application discloses a preparation method of Saccharomyces cerevisiae-derived yeast β-glucan, which comprises the following steps:

[0008] (1) mixing the yeast raw material with a first solvent to obtain a yeast raw material solution;

[0009] (2) subjecting the yeast raw material solution obtained in step (1) to high-pressure homogenization to obtain a homogenized solution;

[0010] (3) adding a food-grade alkali to the homogenized solution obtained in step (2) to perform an extraction reaction, to obtain a reaction extraction liquid;

[0011] (4) subjecting the reaction extraction liquid obtained in step (3) to centrifugation to separate a heavy phase, to obtain a heavy phase insoluble cell wall extract;

[0012] (5) adding a second solvent to the heavy phase insoluble cell wall extract obtained in step (4) to perform resuspension, to obtain an insoluble cell wall extract resuspension liquid;

[0013] (6) adding a complex enzyme preparation to the insoluble cell wall extract resuspension liquid obtained in step (5), adjusting the pH of the reaction system using an acidic reagent, and then performing an enzymatic reaction, to obtain an enzymatic reaction liquid;

[0014] (7) subjecting the enzymatic reaction liquid obtained in step (6) to centrifugation to recover a heavy phase, to obtain a heavy phase insoluble yeast β-glucan, and drying, to obtain an insoluble yeast β-glucan dry powder.

[0015] In some embodiments, in step (1), the yeast raw material is any one or a combination of yeast cells, yeast dry powder, and yeast cell walls; the first solvent is water; and the mass ratio of the yeast raw material to the first solvent is 1.0:(7.0-15.0).

[0016] In some embodiments, preferably, in step (1), the yeast raw material is yeast cell walls.

[0017] In some embodiments, preferably, in step (1), the mass ratio of the yeast raw material to the first solvent is 1.0:(7.0-10.0), further preferably 1.0:(7.0-9.0), and more further preferably 1.0:9.0.

[0018] In some embodiments, in step (2), the operating pressure of the high-pressure homogenization is 600 bar-1200 bar.

[0019] In some embodiments, preferably, in step (2), the operating pressure of the high-pressure homogenization is 600 bar-1000 bar, further preferably 600 bar-800 bar, and more further preferably 800 bar.

[0020] In some embodiments, in step (3), the food-grade base is any one or a combination of sodium hydroxide, potassium hydroxide and lithium hydroxide; the amount of the food-grade base is 5.0% to 10.0% of the mass of the yeast raw material in step (1).

[0021] In some embodiments, preferably, in step (3), the food-grade base is sodium hydroxide.

[0022] In some embodiments, in step (3), the extraction reaction is carried out at a temperature of 80 to 100℃; the extraction reaction is carried out for 2 to 6 hours.

[0023] In some embodiments, preferably, in step (3), the extraction reaction is carried out at a temperature of 90 to 100℃, and more preferably at 100℃.

[0024] In some embodiments, preferably, in step (3), the extraction reaction is carried out for 4 to 6 hours, and more preferably for 6 hours.

[0025] In some embodiments, in step (5), the second solvent is water.

[0026] In step (5), the insoluble cell wall extract obtained in step (4) is resuspended by adding the second solvent, and the amount of the second solvent is such that the solid content in the solution is 2% to 5%, and more preferably 3%.

[0027] In some embodiments, in step (6), the complex enzyme preparation is any one or a combination of protease, lipase, chitinase and mannanase; the amount of the complex enzyme preparation is 0.05% to 0.5% of the mass of the yeast raw material in step (1).

[0028] In some embodiments, preferably, in step (6), the complex enzyme preparation is a combination of protease, lipase, chitinase and mannanase in any mass ratio, and more preferably a combination of protease, lipase, chitinase and mannanase in a mass ratio of (0.8 to 1.2) : (0.8 to 1.2) : (0.8 to 1.2) : (0.8 to 1.2), and even more preferably a combination of protease, lipase, chitinase and mannanase in a mass ratio of 1.0 : 1.0 : 1.0 : 1.0.

[0029] In some embodiments, preferably, in step (6), the amount of the complex enzyme preparation used, based on the mass of the yeast raw material in step (1), is 0.05% to 0.4% of the mass of the yeast raw material, further preferably 0.05% to 0.3%, and more further preferably 0.05% to 0.2%.

[0030] In step (6), the protease can be commercially purchased, and preferably is a pento- ptyptase or an enzyme of the same potency.

[0031] In step (6), the lipase can be commercially purchased, and preferably is a summer food-grade lipase or an enzyme of the same potency.

[0032] In step (6), the chitinase can be commercially purchased, and preferably is a summer complex lysozyme 2000 (lysozyme is a general term for enzymes having lysozyme potency, and this enzyme preparation is identified as chitinase), or an enzyme of the same potency.

[0033] In step (6), the mannanase can be commercially purchased, and preferably is a summer feed-grade mannanase or an enzyme of the same potency.

[0034] In some embodiments, in step (6), the acidic reagent is hydrochloric acid; the pH of the reaction system is adjusted to 3.0 to 7.0 using the acidic reagent; the enzymatic reaction is carried out at a temperature of 30°C to 70°C; and the enzymatic reaction is carried out for a time of 2h to 6h.

[0035] In some embodiments, preferably, in step (6), the acidic reagent is a hydrochloric acid solution, and the concentration of the hydrochloric acid in the solution is 3.0 mol / L to 10.0 mol / L.

[0036] In some embodiments, preferably, in step (6), the pH of the reaction system is adjusted to 4.0 to 6.0, and further preferably to 5.0, using the acidic reagent.

[0037] In some embodiments, preferably, in step (6), the enzymatic reaction is carried out at a temperature of 40°C to 60°C, and further preferably at 50°C.

[0038] In some embodiments, preferably, in step (6), the enzymatic reaction is carried out for a time of 4h to 6h, and further preferably for a time of 5h to 6h, and more further preferably for 6h.

[0039] In step (7), the drying method used can be any one of drum drying, spray drying, freeze drying, and vacuum drying.

[0040] In some embodiments, preferably, in step (7), the drying method is spray drying.

[0041] In some embodiments, in step (7), the purity of the insoluble yeast beta-glucan dry powder is ≥ 72%; preferably, the purity of the insoluble yeast beta-glucan dry powder is ≥ 80%.

[0042] Advantages:

[0043] (1) The preparation method of the yeast glucan provided by the present application shortens the conventional preparation process, greatly alleviates the environmental protection treatment pressure, effectively reduces the production cost, and the purity of the prepared yeast glucan is higher, which has better industrial application prospect.

[0044] (2) The preparation method of the present application uses physical, chemical and biological methods in combination, utilizes the advantages of each method, reduces the production cost and difficulty, avoids the steps that may cause safety problems, and improves the reliability of production.

[0045] (3) The preparation method of the present application is simple, and the equipment for reaction, separation and drying is universal and common, which is easy to realize scale-up production. BRIEF DESCRIPTION OF DRAWINGS

[0046] The above and / or other aspects of the present application will become more apparent by describing in detail the preferred embodiments thereof with reference to the attached drawings in which:

[0047] Figure 1 The following is a typical preparation process of the insoluble yeast beta-glucan in the examples. DETAILED DESCRIPTION

[0048] The present application can be better understood according to the following examples. However, it is easily understood by those skilled in the art that the content described in the examples is only for illustrating the present application, and should not and will not limit the present application described in detail in the claims.

[0049] In the following examples, the experimental methods are conventional methods unless otherwise specified; the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0050] The typical preparation process of the insoluble yeast beta-glucan in the examples is shown in the following figure: Figure 1As shown, the yeast raw material (yeast cell wall) is mixed with water, and then high-pressure homogenization is performed to obtain a homogenized liquid; food-grade alkali is added to the homogenized liquid to perform an extraction reaction, and an extracted liquid is obtained; the extracted liquid is centrifuged to separate a heavy phase, and a heavy-phase insoluble cell wall extract is obtained; the heavy-phase insoluble cell wall extract is resuspended with water, and then a complex enzyme preparation is added; an acidic reagent is used to adjust the pH of the reaction system, and then an enzymatic reaction is performed to obtain an enzymatic reaction liquid; the enzymatic reaction liquid is centrifuged to recover a heavy phase, and a heavy-phase insoluble yeast β-glucan is obtained, which is dried to obtain an insoluble yeast β-glucan dry powder.

[0051] The hydrochloric acid solution used in the embodiments of the present application is an aqueous hydrochloric acid solution, and the concentration of the hydrochloric acid in the hydrochloric acid solution is 3.0 mol / L to 10.0 mol / L, unless otherwise specified.

[0052] The experimental materials / instruments used in the embodiments and the manufacturer information are shown in Table 1 below:

[0053] Table 1

[0054] Reagent / instrument Model Manufacturer Yeast cell wall Gao-fu you 251 Angel yeast Homogenizer AH-BASTC 30 Suzhou anto Water bath WBS-6pro JOANLAB Centrifuge TG16.5 Shanghai Luxiang instrument Rotary evaporator RE-52 Saint leaf

[0055] Protease: Panko alkaline protease; lipase: Xiansheng food-grade lipase; chitinase: Xiansheng complex lysozyme 2000; mannanase: Xiansheng feed-grade mannanase.

[0056] Embodiment 1:

[0057] Prepare yeast raw material (yeast cell wall), pure water, food-grade sodium hydroxide, food-grade hydrochloric acid solution, protease, lipase, chitinase, mannanase, and other raw materials, and high-pressure homogenizer, heating reaction kettle, high-speed centrifuge, spray dryer, and other equipment.

[0058] (1) The yeast raw material (yeast cell wall: Gaofuyu 251, Angel Yeast) is added to 9 times the mass of water, and stirred uniformly to ensure that there are no material agglomerates, wall sticking, and stratification in the liquid, to obtain a yeast raw material liquid.

[0059] (2) The high-pressure homogenizer is used to mix the yeast raw material liquid uniformly at a treatment pressure of 600 bar to obtain a homogenized liquid.

[0060] (3) The homogenized liquid is transferred to the heating reaction kettle, and food-grade sodium hydroxide is added to the homogenized liquid at a mass fraction of 5% of the yeast raw material to perform an extraction reaction, the reaction temperature is 100℃, the extraction time is 6h, and the extracted liquid is obtained after the reaction is completed.

[0061] (4) The extracted liquid is separated using a high-speed centrifuge to recover a heavy phase, and a heavy-phase insoluble cell wall extract is obtained.

[0062] (5) Add clean water to the heavy phase insoluble cell wall extract to resuspend the insoluble cell wall extract, and the amount of clean water is such that the solid content in the slurry is 3% after addition, to obtain an insoluble cell wall extract resuspension.

[0063] (6) Add a complex enzyme preparation to the insoluble cell wall extract resuspension and perform an enzymatic reaction, wherein the complex enzyme preparation is a mixture of 0.05% of the yeast raw material by mass of protease, 0.05% of the yeast raw material by mass of lipase, 0.05% of the yeast raw material by mass of chitinase, and 0.05% of the yeast raw material by mass of mannanase, the reaction system pH is adjusted to 5.0 using a hydrochloric acid solution, and after being fully stirred, the enzymatic reaction is performed by heating, the reaction temperature is 50°C, and the reaction time is 6h, to obtain an enzymatic reaction liquid.

[0064] (7) The enzymatic reaction liquid is separated using a high-speed centrifuge to obtain heavy phase insoluble yeast β-glucan; the heavy phase insoluble yeast β-glucan is dried using a spray dryer to obtain insoluble yeast β-glucan dry powder, and the purity is detected to be 75.47%.

[0065] Example 2:

[0066] (1) Add the yeast raw material (yeast cell wall: Gao Fuxu 251, Angel Yeast) to 7 times the mass of clean water, fully stir to ensure that there are no material lumps, wall sticking, and layering in the slurry, to obtain a yeast raw material slurry.

[0067] (2) The yeast raw material slurry is fully mixed using a high-pressure homogenizer at a treatment pressure of 600bar to obtain a homogenized slurry.

[0068] (3) The homogenized slurry is transferred to a heated reaction kettle, and 5% of the mass of the yeast raw material of food-grade sodium hydroxide is added to perform an extraction reaction, the reaction temperature is 100°C, the extraction time is 6h, and after the reaction is completed, a reaction extraction liquid is obtained.

[0069] (4) The reaction extraction liquid is separated using a high-speed centrifuge, and the heavy phase is recovered to obtain a heavy phase insoluble cell wall extract.

[0070] (5) Add clean water to the heavy phase insoluble cell wall extract to resuspend the insoluble cell wall extract, and the amount of clean water is such that the solid content in the slurry is 3% after addition, to obtain an insoluble cell wall extract resuspension.

[0071] (6) Add a complex enzyme preparation to the insoluble cell wall extract resuspension and perform an enzymatic reaction, wherein the complex enzyme preparation is a mixture of 0.05% of the yeast raw material by mass of protease, 0.05% of the yeast raw material by mass of lipase, 0.05% of the yeast raw material by mass of chitinase, and 0.05% of the yeast raw material by mass of mannanase, the reaction system pH is adjusted to 5.0 using a hydrochloric acid solution, and the enzymatic reaction is performed after being fully stirred and heated, the reaction temperature is 50°C, and the reaction time is 6h, to obtain an enzymatic reaction liquid.

[0072] (7) The enzymatic reaction liquid is separated using a high-speed centrifuge to obtain a heavy phase insoluble yeast β-glucan; the heavy phase insoluble yeast β-glucan is dried using a spray dryer to obtain insoluble yeast β-glucan dry powder, and the purity is detected to be 72.11%.

[0073] Example 3:

[0074] (1) The yeast raw material (yeast cell wall: Gao Fuxu 251, Angel Yeast) is added to 9 times the mass of water, fully stirred and uniformly mixed, and the material is ensured to be free of material clumping, wall sticking, and layering in the liquid to obtain a yeast raw material liquid.

[0075] (2) The yeast raw material liquid is fully mixed using a high-pressure homogenizer at a treatment pressure of 800 bar to obtain a homogenized liquid.

[0076] (3) The homogenized liquid is transferred to a heated reaction kettle, and 10% of the mass of the yeast raw material of food-grade sodium hydroxide is added for extraction reaction, the reaction temperature is 100°C, the extraction time is 6h, and the reaction is completed to obtain a reaction extraction liquid.

[0077] (4) The reaction extraction liquid is separated using a high-speed centrifuge, and the heavy phase is recovered to obtain a heavy phase insoluble cell wall extract.

[0078] (5) Water is added to the heavy phase insoluble cell wall extract to resuspend the insoluble cell wall extract, the amount of water used is such that the solid content in the liquid is 3% after being added, to obtain an insoluble cell wall extract resuspension.

[0079] (6) A complex enzyme preparation is added to the insoluble cell wall extract resuspension and an enzymatic reaction is performed, wherein the complex enzyme preparation is a mixture of 0.05% of the yeast raw material by mass of protease, 0.05% of the yeast raw material by mass of lipase, 0.05% of the yeast raw material by mass of chitinase, and 0.05% of the yeast raw material by mass of mannanase, the reaction system pH is adjusted to 5.0 using a hydrochloric acid solution, and the enzymatic reaction is performed after being fully stirred and heated, the reaction temperature is 50°C, and the reaction time is 6h, to obtain an enzymatic reaction liquid.

[0080] (7) Using a high-speed centrifuge to separate the enzymatic reaction liquid, obtaining the heavy phase insoluble yeast β-glucan; using a spray dryer to dry the heavy phase insoluble yeast β-glucan, obtaining the insoluble yeast β-glucan dry powder, and detecting the purity of 80.03%.

[0081] Example 4:

[0082] (1) The yeast raw material (yeast cell wall: Gao Fuxu 251, Angel Yeast) was added to 9 times the mass of water, and fully stirred to ensure that there was no material caking, wall sticking, and layering in the material liquid, obtaining the yeast raw material liquid.

[0083] (2) Using a high-pressure homogenizer, the yeast raw material liquid was fully mixed at a treatment pressure of 600 bar, obtaining the homogenized liquid.

[0084] (3) The homogenized liquid was transferred to a heated reaction kettle, and 5% of food-grade sodium hydroxide of the mass of the yeast raw material was added for extraction reaction, the reaction temperature was 100°C, the extraction time was 6h, and the reaction ended to obtain the reaction extraction liquid.

[0085] (4) Using a high-speed centrifuge to separate the reaction extraction liquid, recovering the heavy phase, and obtaining the heavy phase insoluble cell wall extract.

[0086] (5) Water was added to the heavy phase insoluble cell wall extract to resuspend the insoluble cell wall extract, and the amount of water was such that the solid content in the liquid was 3% after addition, obtaining the insoluble cell wall extract resuspension.

[0087] (6) A composite enzyme preparation was added to the insoluble cell wall extract resuspension and enzymatic reaction was performed, wherein the composite enzyme preparation was a mixture of 0.10% protease, 0.10% lipase, 0.10% chitinase, and 0.10% mannanase of the mass of the yeast raw material, hydrochloric acid solution was used to adjust the pH of the reaction system to 5.0, and after being fully stirred and uniformly mixed, heating was performed for enzymatic reaction, the reaction temperature was 50°C, and the reaction time was 6h, obtaining the enzymatic reaction liquid.

[0088] (7) Using a high-speed centrifuge to separate the enzymatic reaction liquid, obtaining the heavy phase insoluble yeast β-glucan; using a spray dryer to dry the heavy phase insoluble yeast β-glucan, obtaining the insoluble yeast β-glucan dry powder, and detecting the purity of 78.74%.

[0089] The use amount of the composite enzyme preparation was increased in the present comparative example, and although the purity was increased, the increase was limited and not large relative to the amount of enzyme.

[0090] Comparative Example 1:

[0091] (1) The yeast raw material (yeast cell wall: Gaoaoyou 251, Angel Yeast) was added to 9 times the mass of water, and stirred thoroughly to ensure that there were no material agglomerations, wall sticking, or layering in the material liquid, to obtain a yeast raw material liquid.

[0092] (2) The yeast raw material liquid was mixed thoroughly using a high-pressure homogenizer at a treatment pressure of 600 bar to obtain a homogenized liquid.

[0093] (3) The homogenized liquid was transferred to a heated reaction kettle, and 3% of food-grade sodium hydroxide based on the mass of the yeast raw material was added for extraction reaction, the reaction temperature was 100°C, and the extraction time was 6 h, and after the reaction was completed, a reaction extraction liquid was obtained.

[0094] (4) The reaction extraction liquid was separated using a high-speed centrifuge, and the heavy phase was recovered to obtain a heavy phase insoluble cell wall extract.

[0095] (5) Water was added to the heavy phase insoluble cell wall extract to resuspend the insoluble cell wall extract, and the amount of water used was such that the solid content in the liquid after addition was 3%, to obtain an insoluble cell wall extract resuspension liquid.

[0096] (6) A complex enzyme preparation was added to the insoluble cell wall extract resuspension liquid and enzymatic reaction was performed, wherein the complex enzyme preparation was a mixture of 0.05% protease, 0.05% lipase, 0.05% chitinase, and 0.05% mannanase based on the mass of the yeast raw material, hydrochloric acid solution was used to adjust the pH of the reaction system to 5.0, and after thorough stirring, the reaction was heated to perform enzymatic reaction, the reaction temperature was 50°C, and the reaction time was 6 h, to obtain an enzymatic reaction liquid.

[0097] (7) The enzymatic reaction liquid was separated using a high-speed centrifuge to obtain heavy phase insoluble yeast β-glucan; the heavy phase insoluble yeast β-glucan was dried using a spray dryer to obtain insoluble yeast β-glucan dry powder, and the purity was 67.50%.

[0098] The amount of food-grade sodium hydroxide was reduced in this comparative example, and the purity was significantly reduced compared to Example 1, so the amount of alkali should not be further reduced.

[0099] Comparative Example 2:

[0100] (1) The yeast raw material (yeast cell wall: Gaoaoyou 251, Angel Yeast) was added to 9 times the mass of water, and stirred thoroughly to ensure that there were no material agglomerations, wall sticking, or layering in the material liquid, to obtain a yeast raw material liquid.

[0101] (2) The yeast raw material liquid was mixed thoroughly using a high-pressure homogenizer at a treatment pressure of 600 bar to obtain a homogenized liquid.

[0102] (3) The homogeneous liquid is transferred to a heated reaction kettle, and 12% of food-grade sodium hydroxide by mass of the yeast raw material is added for extraction reaction, the reaction temperature is 100 DEG C, the extraction time is 6h, and the reaction extraction liquid is obtained after the reaction is completed.

[0103] (4) The reaction extraction liquid is separated using a high-speed centrifuge, and the heavy phase is recovered to obtain a heavy phase insoluble cell wall extract.

[0104] (5) Water is added to the heavy phase insoluble cell wall extract to resuspend the insoluble cell wall extract, the amount of water is such that the solid content in the liquid after addition is 3%, and the insoluble cell wall extract resuspension liquid is obtained.

[0105] (6) A complex enzyme preparation is added to the insoluble cell wall extract resuspension liquid, and an enzymatic reaction is carried out, wherein the complex enzyme preparation is a mixture of 0.05% of protease, 0.05% of lipase, 0.05% of chitinase, and 0.05% of mannanase by mass of the yeast raw material, the pH of the reaction system is adjusted to 5.0 using hydrochloric acid solution, and the enzymatic reaction is carried out after being fully stirred and heated, the reaction temperature is 50 DEG C, the reaction time is 6h, and the enzymatic reaction liquid is obtained.

[0106] (7) The enzymatic reaction liquid is separated using a high-speed centrifuge to obtain a heavy phase insoluble yeast beta-glucan; the heavy phase insoluble yeast beta-glucan is dried using a spray dryer to obtain insoluble yeast beta-glucan dry powder, and the purity is 73.25%.

[0107] The amount of food-grade sodium hydroxide is increased in the present comparative example, and the purity is slightly reduced compared with example 1. The increase of the amount of alkali will cause the enhancement of Maillard reaction and the increase of impurities, so the amount of alkali should not be further increased.

[0108] The present application provides a preparation method of yeast beta-glucan from Saccharomyces cerevisiae, and there are many methods and ways to realize the technical scheme, and the above description is only the preferred embodiment of the present application. It should be pointed out that for ordinary skilled persons in the technical field, some improvements and refinements can be made without departing from the principle of the present application, and these improvements and refinements should be regarded as the protection scope of the present application. The components not explicitly described in the present embodiment can be realized by using existing technology.

Claims

1. A method for the production of a Saccharomyces cerevisiae-derived yeast β-glucan, characterized in that, The method comprises the following steps: (1) mixing yeast raw material with a first solvent to obtain a yeast raw material solution; (2) subjecting the yeast raw material solution obtained in step (1) to high-pressure homogenization to obtain a homogenized solution; (3) adding a food-grade alkali to the homogenized solution obtained in step (2) to perform an extraction reaction, thereby obtaining a reaction extraction solution; (4) subjecting the reaction extraction solution obtained in step (3) to centrifugation to separate a heavy phase, thereby obtaining a heavy phase insoluble cell wall extract; (5) adding a second solvent to the heavy phase insoluble cell wall extract obtained in step (4) to perform resuspension, thereby obtaining an insoluble cell wall extract resuspension solution; (6) adding a composite enzyme preparation to the insoluble cell wall extract resuspension solution obtained in step (5), adjusting the pH of the reaction system using an acidic reagent, and then performing an enzymatic reaction, thereby obtaining an enzymatic reaction solution; (7) subjecting the enzymatic reaction solution obtained in step (6) to centrifugation to recover a heavy phase, thereby obtaining a heavy phase insoluble yeast β-glucan, and drying the heavy phase insoluble yeast β-glucan, thereby obtaining a dry insoluble yeast β-glucan powder.

2. The production method according to claim 1, characterized by, In step (1), the yeast raw material is any one or a combination of yeast cells, yeast dry powder and yeast cell walls; the first solvent is water; and the mass ratio of the yeast raw material to the first solvent is 1.0:(7.0-15.0).

3. The production method according to claim 1, characterized by, In step (2), the operating pressure of the high-pressure homogenization is 600 bar-1200 bar.

4. The method of claim 1, wherein, In step (3), the food-grade alkali is any one or a combination of sodium hydroxide, potassium hydroxide and lithium hydroxide; the amount of the food-grade alkali, based on the mass of the yeast raw material in step (1), is 5.0%-10.0% of the mass of the yeast raw material.

5. The preparation method according to claim 1, characterized in that, In step (3), the extraction reaction is performed at a temperature of 80-100°C for 2 h-6 h.

6. The method of claim 1, wherein, In step (5), the second solvent is water.

7. The preparation method according to claim 1, characterized in that, In step (6), the composite enzyme preparation is any one or a combination of protease, lipase, chitinase and mannanase; the amount of the composite enzyme preparation, based on the mass of the yeast raw material in step (1), is 0.05%-0.5% of the mass of the yeast raw material.

8. The method of claim 1, wherein, In step (6), the acidic reagent is hydrochloric acid; the pH of the reaction system is adjusted to 3.0-7.0 using the acidic reagent; the enzymatic reaction is performed at a temperature of 30°C-70°C for 2 h-6 h.

9. The method of claim 1, wherein, In step (7), the drying method is any one of roller drying, spray drying, freeze drying and vacuum drying.

10. The method of claim 1, wherein, In step (7), the purity of the dry insoluble yeast β-glucan powder is ≥72%; preferably, the purity of the dry insoluble yeast β-glucan powder is ≥80%.

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