Method for preventing and treating monochamus alternatus by using eyespot nail knocking eggs

Abstract

The invention relates to the field of pest prevention and control, in particular to a method for preventing and controlling monochamus alternatus by utilizing eyespot beetle knocking eggs, which comprises the following steps: 1) collecting eyespot beetle knocking imagoes in a pine forest; (2) continuously feeding the collected eyeshield adult beetles in the breeding box; (3) collecting eggs laid by the adult eyeshield beetles; and (4) releasing the collected ocultinia oculi eggs to a pine forest area in which monochamus alternatus pests are to be prevented and controlled. According to the method, the population density of the monochamus alternatus in the pine forest region is continuously controlled to be at a low level by utilizing the food chain advantage of the percussion oculi, and compared with a chemical mode for preventing and controlling the monochamus alternatus in the prior art, the technical scheme provided by the invention has the great advantages of environmental protection, strong prevention and control effect persistence and low technical cost, and is suitable for popularization and application in the field of prevention and control of the monochamus alternatus in the pine forest region. The method is suitable for large-scale popularization and application.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of pest control, in particular to a method for controlling Monochamus alternatus by using eggs of Melanotus oregonensis. BACKGROUND

[0002] The pine wood nematode disease is a kind of devastating forest disease caused by the pine wood nematode parasitizing in pine trees, which leads to the rapid death of pine trees. The pine wood nematode disease spreads rapidly, has a wide distribution of host plants, and is difficult to control, so it is listed as the first plant quarantine object in the world and is called the "cancer" of pine trees.

[0003] The spread of the pine wood nematode is mainly by media insects such as Monochamus alternatus, therefore, controlling the media is an effective way to control the spread of the pine wood nematode disease. Monochamus alternatus damages pine trees inside, and its life is hidden, so it is difficult to control. At present, the techniques for controlling Monochamus alternatus include cleaning of infected wood, trapping of adults, and spraying of chemical agents by airplane. However, the above methods have high control cost and short control effectiveness. Therefore, the ecological control technology of Monochamus alternatus, which increases the biodiversity of forest enemies and gradually achieves the ecological balance based on the food chain, can sustainably control the population density of Monochamus alternatus at a low level, which is a major scientific and technological problem that we must solve. SUMMARY

[0004] In view of the above defects, the present application aims to provide a method for controlling Monochamus alternatus by using eggs of Melanotus oregonensis.

[0005] To achieve the above-mentioned purpose, the present application provides a method for controlling Monochamus alternatus by using eggs of Melanotus oregonensis, which comprises the following steps: 1) collecting Melanotus oregonensis adults in pine forests; 2) continuing to breed the collected Melanotus oregonensis adults in a breeding box; 3) collecting the eggs produced by the Melanotus oregonensis adults; 4) releasing the collected Melanotus oregonensis eggs into a pine forest area where Monochamus alternatus pest control is needed.

[0006] Preferably, in step 1), the Melanotus oregonensis adults are collected by hanging a trap in the pine forest, wherein a trapping agent is placed in the trap.

[0007] Preferably, the trapping agent comprises the following components in mass percentage: (1R)-2.2-dimethyl-3-methylenebicycloalkane: 10%-20%, pinene: 10%-20%, carene: 5%-15%, myrcene: 10%-20%, hexenal: 5%-15%, γ-terpinene: 1%-3%, propionic acid geranyl ester: 0.5%-2%, and the balance is 95% ethanol to make up to 100%.

[0008] Preferably, in step 3), the pine bark is provided in the breeding box for the adults of the Anisocacia obliqua to lay eggs, and the egg masses are collected once every 3-7 days; the pine bark with the egg masses is cut into pieces and placed in the egg release bags, and the number of the eggs in each egg release bag is controlled to be 30-50.

[0009] Preferably, the egg release bag is a PE self-sealing bag, and the size of the PE self-sealing bag is 4-5 cm in length and 5-6 cm in width; the two lower sides of the PE self-sealing bag are cut to form a release opening in the shape of a triangle with a side length of 3-5 mm.

[0010] Preferably, the collected eggs of the Anisocacia obliqua are released into the pine forest area to be controlled by the Monochamus alternatus by the per-tree release method and / or the wood pile release method.

[0011] Preferably, the per-tree release method is to fix the egg release bag on the trunk of a pine tree in the area to be controlled, and one release bag is placed on each pine tree.

[0012] Preferably, the wood pile release method is to set a plurality of pine wood piles in the pine forest area to be controlled, and after 1-2 months, the egg release bag is fixed on the wood segment of the pine wood pile, and 1-4 release bags are placed on each pine wood pile.

[0013] Preferably, the distance between the adjacent pine wood piles is greater than 50 m.

[0014] The present application directly captures the adults of the Anisocacia obliqua in the pine forest to efficiently obtain the eggs of the Anisocacia obliqua in a short time; the collected eggs of the Anisocacia obliqua are released into the pine forest area to be controlled by the Monochamus alternatus, and the eggs are hatched and grow as soon as possible in combination with the environment of the pine forest area, so that the population density of the Anisocacia obliqua in the pine forest area is rapidly increased, and the population density of the Monochamus alternatus in the pine forest area is continuously controlled at a low level by using the food chain advantage of the Anisocacia obliqua. In addition, the population of the Anisocacia obliqua is continuously bred in the pine forest control area, and a relatively stable ecological circle is formed with the low population of the Monochamus alternatus, so that the effect of controlling the Monochamus alternatus in the pine forest area is sustainable, and the pine forest ecology is truly protected. Compared with the chemical method for controlling the Monochamus alternatus in the prior art, the technical scheme provided by the present application has the advantages of environmental protection, strong persistence of control effect, and low technical cost, and is suitable for large-scale popularization and application. DETAILED DESCRIPTION

[0015] The specific embodiments of the technical scheme claimed in the present application will be described below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative labor fall within the scope of protection of the present application.

[0016] It should be noted that the technical solutions among various embodiments can be combined with each other, but it must be based on that a person skilled in the art can realize, when the combination of technical solutions appears contradictory or unachievable, it should be considered that the combination of technical solutions does not exist, and is not within the protection scope required by the present application.

[0017] It is found that the eye-spotted pine borer is a common dominant natural enemy of the monochamus alternatus, and has a significant control effect on the population quantity of the monochamus alternatus. However, the life history of the eye-spotted pine borer is long, at least more than two years, resulting in high feeding cost, and at present, it is still not possible to be applied on a large scale. Further investigation finds that the population density of the eye-spotted pine borer is large in the pine forest, but under natural conditions, the adult life span of the eye-spotted pine borer is short, and the egg production quantity is small. In order to efficiently and sustainably utilize the eye-spotted pine borer eggs to prevent and treat the monochamus alternatus pest in the pine forest area, the present application provides a method, including the following steps: 1) collecting the eye-spotted pine borer adults in the pine forest; 2) continuing to feed the collected eye-spotted pine borer adults in a breeding box; 3) collecting the eggs produced by the eye-spotted pine borer adults; 4) releasing the collected eye-spotted pine borer eggs to the pine forest area to be prevented and treated from the monochamus alternatus pest.

[0018] The present application directly captures the eye-spotted pine borer adults in the pine forest, so as to efficiently obtain the eye-spotted pine borer eggs in a short time. Then, the collected eye-spotted pine borer eggs are released to the pine forest area to be prevented and treated from the monochamus alternatus pest, and the environment of the pine forest area is combined to make the eye-spotted pine borer eggs hatch and grow as soon as possible, so as to rapidly increase the population density of the eye-spotted pine borer in the above-mentioned pine forest area, thereby utilizing the food chain advantage to sustainably control the population density of the monochamus alternatus in the pine forest area at a lower level. Since the eye-spotted pine borer population can sustainably breed in the pine forest prevention area, a relatively stable ecological circle is formed with the monochamus alternatus pest at a lower level, so that the effect of preventing and treating the monochamus alternatus in the pine forest area is sustainable, and the pine forest ecology is truly protected. Compared with the chemical method for preventing and treating the monochamus alternatus in the prior art, the technical solution provided by the present application has the great advantages of environmental protection, strong persistence of prevention and treatment effect, and low technical cost, and is suitable for large-scale popularization and application.

[0019] Preferably, in the step 1), the eye-spotted pine borer adults are collected by hanging a trap in the pine forest area, and the trap is provided with a trapping agent, which is convenient and efficient.

[0020] Further, the trapping agent comprises the following mass percentage of components: (1R)-2.2-dimethyl-3-methylenebicycloalkane: 10%-20%, pinene: 10%-20%, carene: 5%-15%, myrcene: 10%-20%, hexenal: 5%-15%, gamma-terpinene: 1%-3%, propionic acid geranyl ester: 0.5%-2%, and the balance is 95% ethanol to make up to 100%, which has good attraction effect and persistence period on the adult of the eye-spotted pinhole beetle, and also has good attraction effect on the monochamus alternatus, and the two types of insects are simultaneously attracted to the trap, and part of the monochamus alternatus can be directly eliminated, and the eye-spotted pinhole beetle adult with excellent body function can be roughly screened to lay eggs for the next step to obtain high-quality eye-spotted pinhole beetle eggs.

[0021] Preferably, in the above step 3), pine bark is provided in the breeding box for the eye-spotted pinhole beetle to lay eggs, and the egg blocks are collected every 3-7 days; the pine bark with egg blocks is cut into multiple blocks and placed in the egg release bag, and the number of insect eggs in each egg release bag is controlled to be 30-50.

[0022] Further, the above egg release bag is a PE self-sealing bag with a size specification of 4-5 cm in length and 5-6 cm in width, which can be easily obtained through conventional shopping, and on the basis of the purchased PE self-sealing bag, the two lower sides of the PE self-sealing bag are cut to form a release port with a triangular corner with a side length of 3-5 mm to accurately release the eye-spotted pinhole beetle eggs onto the pine tree trunks in the control area.

[0023] As for the specific operation mode of releasing the collected eye-spotted pinhole beetle eggs into the pine forest area to be controlled for the monochamus alternatus, there are two different implementation schemes of per-wood release method and wood pile release method, and the two schemes can be implemented alone in the same control pine forest area, or can be implemented in parallel and adaptively in the same control pine forest area, and can be flexibly selected according to the actual situation of the control pine forest area, efficiently and sustainably solving the monochamus alternatus pest.

[0024] Further, the specific operation mode of the per-wood release method is to fix the egg release bag on the pine trunk in the area to be controlled, and one release bag is placed on each pine tree.

[0025] Further, the specific operation mode of the wood pile release method is to set multiple pine wood piles in the pine forest area to be controlled for the monochamus alternatus, and after 1-2 months, the egg release bag is fixed on the wood segment of the pine wood pile, and 1-4 release bags are placed in each pine wood pile.

[0026] Preferably, in the multiple pine wood piles, the distance between adjacent pine wood piles is more than 50 m.

[0027] To verify the pine moth pest control effect of each embodiment of the present application, six different pine forest bases are delineated for field verification, and the specific test process is as follows: Test one. In Wuling Forest Farm, 60 mu of pine forest is selected, and in June 2023, 25 wood piles are set in the forest, each pile is more than 50 m apart, 100 bags of eye stripe click beetle egg bags are released, with about 3000 eggs; that is, each wood pile occupies an area of 2.4 mu, and 120 eye stripe click beetle eggs are released per wood pile, so that the average release of eye stripe click beetle eggs per mu in the forest farm is 50. Through dissection investigation in November 2023, the ratio of the emergence hole of the pine moth to the egg laying groove in the above wood pile is 13.5%.

[0028] It should be noted that the emergence hole of the pine moth is the mark left by the adult pine moth after emergence, and the egg laying groove is the mark of the female insect laying eggs. By counting the ratio of the two, the population size and breeding activity of the pine moth can be indirectly reflected. The increase of the ratio may indicate that the risk of spread of pine wood nematode disease increases, and timely control measures need to be taken. The significant decrease in the ratio indicates that the control measures have achieved good results.

[0029] Test two. In Shitang Village of Shatou Town, 100 mu of pine forest is selected, and in July 2023, 40 wood piles are set in the pine forest, each pile is more than 50 m apart, 180 bags of eye stripe click beetle egg bags are released, with about 7000 eggs, that is, each wood pile occupies an area of 2.5 mu, and 175 eye stripe click beetle eggs are released per wood pile, so that the average release of eye stripe click beetle eggs per mu in the forest farm is 70. Through dissection investigation in November 2023, the ratio of the emergence hole of the pine moth to the egg laying groove in the above wood pile is 9.4%.

[0030] Test three. In Xizhong Village of Muduan Town, 50 mu of pine forest is selected, and in August 2023, 20 wood piles are set in the pine forest, each pile is more than 50 m apart, 100 bags of eye stripe click beetle egg bags are released, with about 4000 eggs, that is, each wood pile occupies an area of 2.5 mu, and 200 eye stripe click beetle eggs are released per wood pile, so that the average release of eye stripe click beetle eggs per mu in the forest farm is 80. Through dissection investigation in November 2023, the ratio of the emergence hole of the pine moth to the egg laying groove in the above wood pile is 7.6%.

[0031] Test four. In Peizhong Village of Shiqiao Town, 50 mu of pine forest is selected, and in early April 2023, 400 bags of eye stripe click beetle egg bags are released, with about 15,000 eggs, each wood releases 1 bag, and the release of the wood accounts for 27% of the wood. In October 2023, the mortality rate of pine trees decreased by 64.28% compared with the same period last year.

[0032] Test five. In Shiqiao Town Shengli Village, 50 mu of pine forest was selected, and 300 bags of egg bags of Melanotus ophthalmicus were released in early April 2023, 1 bag per tree, and the release of the tree accounted for 20% of the total trees, with a total of about 10,000 eggs. In October 2023, the mortality rate of pine trees decreased by 60% compared with the same period last year.

[0033] Test six. In Liushi Village, Nandu Town, 50 mu of pine forest was selected, and 600 bags of egg bags of Melanotus ophthalmicus were released in early April 2023, 1 bag per tree, and the release of the tree accounted for 45% of the total trees, with a total of about 20,000 eggs. In October 2023, the mortality rate of pine trees decreased by 75% compared with the same period last year.

[0034] The above-mentioned tests one to three use the lure wood pile release method to release the collected Melanotus ophthalmicus eggs to different 3 pine forest areas, and from the process of the three tests and the actual recorded test results, it can be seen that the more the number of Melanotus ophthalmicus eggs released per mu of pine forest, the better the control effect of pine wood nematode; Tests four to six use the per-tree release method to release the collected Melanotus ophthalmicus eggs to different 3 pine forest areas, and from the process of the three tests and the actual recorded test results, the proportion of the release of the tree to the total tree is high, and the control effect of pine wood nematode is good, and the area effect in the test is not great.

[0035] The above-mentioned is only the preferred embodiment of the present application, and does not limit the patent range of the present application, and any equivalent structural transformation made under the inventive concept of the present application, or direct / indirect application in other related technical fields is included in the patent protection range of the present application.

Claims

1. A method for controlling the pine sawyer beetle using eye-spotted beetle eggs, characterized in that: Includes the following steps: 1) Collect adult eye-spotted click beetles from pine forests; 2) Continue to raise the collected adult eye-spotted click beetles in the rearing boxes; 3) Collect the eggs laid by the adult eye-spotted click beetle; 4) Release the collected eggs of the eye-spotted beetle into the pine forest area where the pine sawyer beetle pest needs to be controlled.

2. The method for controlling the pine sawyer beetle using eye-spotted beetle eggs according to claim 1, characterized in that: In step 1), adult eye-spotted click beetles are collected by hanging traps in the pine forest, with a bait placed inside the traps.

3. The method for controlling the pine sawyer beetle using eye-spotted beetle eggs according to claim 2, characterized in that: The trapping agent comprises the following components in the following mass percentages: 1R)-2,2-dimethyl-3-methylenedicycloane: 10%-20%, pinene: 10%-20%, carene: 5%-15%, myrcene: 10%-20%, hexenal: 5%-15%, γ-terpinene: 1%-3%, geraniol propionate: 0.5%-2%, with the balance being 95% ethanol to bring the total to 100%.

4. The method for controlling the pine sawyer beetle using eye-striped beetle eggs according to claim 1, characterized in that: In step 3), pine bark is provided in the breeding box for adult eye-spotted click beetles to lay eggs, and egg masses are collected every 3-7 days; the pine bark containing egg masses is cut into multiple pieces and placed in egg release bags, with the number of eggs in each egg release bag controlled at 30-50.

5. A method for controlling the pine sawyer beetle using eye-spotted beetle eggs according to claim 3, characterized in that: The egg release bag is a PE self-sealing bag with dimensions of 4-5cm in length and 5-6cm in width. Triangular corners with sides of 3-5mm are cut off from the two lower sides of the PE self-sealing bag to form a release opening.

6. A method for controlling the pine sawyer beetle using eye-striped beetle eggs according to claim 4, characterized in that: The collected eggs of the eye-spotted click beetle were released into pine forests where the pine sawyer beetle pest was to be controlled, using the per-tree release method and / or the log-bait release method.

7. A method for controlling the pine sawyer beetle using eye-striped beetle eggs according to claim 6, characterized in that: The per-tree release method involves fixing egg release bags to the trunks of pine trees in the area to be treated, with one release bag placed on each pine tree.

8. A method for controlling the pine sawyer beetle using eye-striped beetle eggs according to claim 6, characterized in that: The log trap release method involves setting up multiple pine log traps in the pine forest area where the pine sawyer beetle is to be controlled. After 1-2 months, release bags are fixed to the logs of the pine log traps, with 1-4 release bags placed on each pine log trap.

9. A method for controlling the pine sawyer beetle using eye-striped beetle eggs according to claim 8, characterized in that: Among the multiple pine wood bait piles set up, the distance between adjacent pine wood bait piles is more than 50m.