Sample container with FEP film for carrying biological samples for optical microscopic observation
By using a plasma-activated FEP film as the bottom of the sample container, the problem of reduced image resolution caused by glass-bottom containers was solved, achieving high-resolution and low-cost microscopic observation.
CN121013972APending Publication Date: 2025-11-25LIGHT INNOVATION TECH LTD
0 Cites 0 Cited by
Patent Information
- Application Number
- CN202480023700.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-03-29
- Filing Date
- 2024-03-28
- Publication Date
- 2025-11-25
AI Technical Summary
Technical Problem
The difference in refractive index between traditional glass-bottomed containers and water reduces the resolution of biological sample images when observed under an optical microscope. Furthermore, replacing them with lens-based sample containers is costly and not suitable for reuse.
Method used
Using an FEP film as the bottom of the sample container, the surface is activated by plasma treatment to improve the adhesion of biological samples and match the refractive index of water to reduce image distortion.
Benefits of technology
It improves the image resolution of biological samples during microscopic observation, while reducing the manufacturing cost of sample containers and making them suitable for repeated use.
✦ Generated by Eureka AI based on patent content.
Abstract
In optical microscopy including fluorescence microscopy, a biological sample (215) can be immersed in an aqueous liquid medium (210) during observation. Due to the refractive index mismatch between the liquid medium and a conventional glass bottom container holding the sample, image resolution is reduced when viewing the sample. To improve resolution, a sample container (100) is formed with a container body (110) having a bottom (120) with an aperture (125), a fluorinated ethylene propylene copolymer (FEP) film (150) attached to the bottom (120) to cover the aperture (125) to form a cavity-facing surface (155) on the film (150), and exposed to the liquid medium (210). Since the refractive index of FEP is close to that of water, image resolution is improved. Furthermore, the cavity-facing surface (155) is activated by plasma treatment to promote adhesion of the cavity-facing surface (155) to the biological sample (215) to enable cells in the biological sample (215) to proliferate in adherent cell culture and to immobilize the biological sample (215) during observation.
Need to check novelty before this filing date? Find Prior Art