Method for asexual rapid propagation of idesia polycarpa seedlings by using rhizomes

Abstract

The invention relates to the technical field of plant asexual propagation, in particular to a method for asexual rapid propagation of idesia polycarpa seedlings by using rhizomes. Comprising the following steps: S1, preparing breeding materials; s2, breeding material pretreatment; s3, rhizome breeding; and S4, asexual rapid propagation nursery stock outplanting. Compared with the prior art, the method has the advantages that asexual breeding of the idesia polycarpa is carried out by utilizing the rhizome part, and the technical problems that in the prior art, the asexual breeding technology has high requirements for technicians and technical equipment, and the production cost is high are solved.

Description

Technical Field

[0001] This invention belongs to the field of plant asexual propagation technology, and specifically relates to a method for rapid asexual propagation of *Vernicia fordii* seedlings using rhizomes. Background Technology

[0002] Mountain tung tree (Il. polycarpa Maxim.) is a tall tree belonging to the genus Il. polycarpa in the family Elaeagnaceae. Its fruit is red or yellow, resembling bunches of grapes. Both the pericarp and seeds contain oil. Surveys have shown that Guizhou has abundant wild resources of this species. Mountain tung tree has shallow roots, a well-developed root system, and strong adaptability. It is an excellent tree species for soil and water conservation and rocky desertification control. Mountain tung tree is a fast-growing, broad-leaved tree species and a preferred species for mixed coniferous and broad-leaved planting to improve forest quality. It is also a high-quality national reserve woody grain and oil tree species, which is of great significance for improving the agricultural and rural environment, increasing farmers' income, and contributing to rural revitalization.

[0003] Based on the biological characteristics of *Vernicia fordii*, a comprehensive evaluation of the overall benefits of *Vernicia fordii* trees, fruits, and landscape is conducted. Wild *Vernicia fordii* resources are primarily found as single trees with varying fruit-bearing conditions; large groves are rare, as are high-quality mother trees with continuous fruiting and good fruit clusters. Furthermore, the collection of single, tall *Vernicia fordii* mother trees still requires manual climbing, which is dangerous, difficult, and costly. Improper collection methods can also cause significant damage to the mother trees. Therefore, utilizing the limited high-quality propagation materials to conduct rapid clonal propagation of *Vernicia fordii* is of great significance. Currently known practical methods for propagating *Vernicia fordii* mainly focus on seed propagation and tissue culture propagation. Seed propagation has limitations given the limited local seed resources. While tissue culture propagation can achieve rapid propagation, it requires taking plant tissue, sterilizing it in a tissue culture room, hardening it off, and then transplanting it into the field. Except for specialized tissue culture labs, researchers, and companies, most large-scale farmers, cooperatives, and ordinary households lack the technical expertise. Furthermore, the complex procedures and high cost of purchasing small seedlings (typically 15 yuan per plant) mean that this method requires highly skilled personnel and advanced equipment, resulting in high production costs. Its widespread applicability requires further investigation.

[0004] Therefore, this paper provides a method for the asexual and rapid propagation of *Vernicia fordii* seedlings using rhizomes, in order to enrich the propagation methods of *Vernicia fordii* seedlings and solve the current shortcomings. Summary of the Invention

[0005] This invention aims to provide a method for the rapid asexual propagation of *Vernicia fordii* seedlings using rhizomes, mainly to solve the technical problems of existing asexual propagation techniques, which require high levels of technical personnel and equipment and have high production costs.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: A method for rapid asexual propagation of *Vernicia fordii* seedlings via rhizomes includes the following steps: S1: Preparation of propagation materials: In conjunction with the release of seedlings of *Vernicia fordii* this winter and next spring, select healthy, disease-free one-year-old or perennial seedlings of *Vernicia fordii* that have been released from seedlings and have been propagated from seeds of superior varieties and local superior plants, as the mother plant propagation material for the asexual and rapid propagation of *Vernicia fordii* seedlings. S2: Pretreatment of propagation material: Take the rhizome part of the mother plant propagation material of *Vernicia fordii* seedlings, collect the excess roots of the seedlings, collect roots of about 0.5cm-2cm, apply wax to the cut surfaces, and then store them in sand. During storage, pay attention to ventilation, moisture and prevention of root rot, and keep them for use when carrying out root propagation. S3: Tissue propagation: Before spring budding, take out the stored root segments, remove the rotten parts, cut them into small root segments, and soak them in mixed solution A for 2-3 hours. Then, arrange them at a spacing of 20cm×30cm, insert the lower part of the root into the prepared nursery ground at a certain angle, cover with fine soil to bury the root segments, gently compact it to ensure close contact between the root segments and the soil, and cover with straw or other covering materials, provide appropriate shade, and then carry out seedling management work such as watering, fertilizing, weeding, and pest and disease control in a timely manner. S4: Asexual rapid propagation of seedlings for sale: When the seedlings propagated from the rhizomes have a ground diameter of more than 0.5cm and a height of more than 50cm, select healthy seedlings free from diseases and pests, and promptly dig them up and sell them in the nursery during rainy weather, and carry out afforestation work on the mountain.

[0007] Furthermore, in step S1, the selected *Vernicia fordii* seedlings have a ground diameter greater than 0.8 cm and a seedling height greater than 1.0 m.

[0008] Furthermore, in step S1, for the selected and prepared *Vernicia fordii* seedling propagation materials, one-year-old *Vernicia fordii* seedlings with clear and traceable mother plant traits and seed origin are selected.

[0009] Furthermore, in step S2, the pretreatment of propagation materials is carried out near the nursery of asexually propagated seedlings. During the segmented pretreatment, attention should be paid to checking the preservation of roots, stems, etc. If any rotten parts are found, they should be removed in time. Water should be applied in time when water is scarce, but excessive watering should be avoided.

[0010] Further, in step S3, the mixture A contains the following components by weight fraction: 3 parts of 12% indomethacin rooting agent, 40 parts of chlorothalonil, and 20,000 parts of water.

[0011] Furthermore, in step S3, the length of the small root segment is 10-15 cm.

[0012] Furthermore, in step S3, the lower part of the root is inserted into the prepared nursery ground at a certain angle, where a certain angle refers to inserting it at an angle of about 45 degrees.

[0013] Furthermore, in step S3, if a bud can be found on the root segment at the lower end, ensure that the bud is retained on the upper soil surface.

[0014] The beneficial effects of this invention are as follows: 1. The present invention discloses a method for the asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes. This method is a production plant rapid propagation method with low technical difficulty, easy to promote and master. It does not require high-tech equipment or technical personnel. It can be completed with only simple propagation materials and relatively good nursery land. Compared with other asexual propagation methods such as tissue culture, it can be completed by training local people as technicians. It has low requirements for technical personnel, is more scalable, and has lower technical and raw material costs. It is a masterable and scalable asexual rapid propagation technology.

[0015] 2. The breeding mother material used in this invention is a high-quality *Vernicia fordii* mother plant with traceable and tangible seed sources. It belongs to the asexual rapid propagation method of second-generation mother plant material selected after the development of sexual propagation. In production, it can quickly propagate excellent offspring single plants and can carry out the propagation of excellent *Vernicia fordii* single plants at fixed locations and in fixed quantities. It has the advantages of obvious offspring inheriting the advantages of the mother plant and practical propagation method.

[0016] 3. The asexual propagation method for seedlings adopted in this invention, including root burial for rapid asexual propagation, can better combine the well-developed root characteristics of *Vernicia fordii*, and better utilize *Vernicia fordii* seedlings in segments, achieving the effect of efficient utilization of the entire seedling without waste. In production, it has achieved high-value utilization of the plant to a certain extent, laying the foundation for the green production and sustainable development of *Vernicia fordii*.

[0017] 4. Treating the rhizomes with Mixture A can greatly improve the survival rate of the rhizomes and increase the seedling output rate. Detailed Implementation

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] In the description of this invention, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. Where the terms "first," "second," and "third" are used for descriptive purposes and to distinguish technical features, they should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the sequential relationship of the indicated technical features.

[0020] The embodiments thereof are described below based on the overall content of the present invention.

[0021] From November 2023 to December 2024, asexual rapid propagation of *Vernicia fordii* seedlings was carried out in Huichuan District, Zunyi City.

[0022] Example 1: A method for the asexual and rapid propagation of *Vernicia fordii* seedlings using stem segments includes the following steps: S1: Preparation of breeding materials: In November 2023, when the seedlings of *Vernicia fordii* were released from the nursery in Huichuan District, healthy one-year-old or perennial seedlings that were bred from seeds of superior varieties and local superior plants and were free from diseases and pests were selected from the seedlings that had been released (with a ground diameter greater than 0.8 cm and a seedling height greater than 1.0 m) as the parent material for the asexual rapid propagation of *Vernicia fordii* seedlings. In early to mid-March 2024, one-year-old branches with plump and well-developed buds from the upper and middle parts of the outer canopy of domesticated *Vernicia fordii* tissue culture in Huichuan District and local superior fruiting female trees No. 158, 98, and 103 selected in Huichuan District were selected as scion propagation materials for the asexual rapid propagation of *Vernicia fordii* seedlings. S2: Pretreatment of propagation materials: Near the nursery in Sidu Town, Huichuan District, pretreatment of the mother plant propagation materials of *Vernicia fordii* seedlings was carried out in three stages, including: The first section is the rhizome. Collect the excess roots of the seedlings, and collect roots of about 0.5cm-2cm. After applying wax to the cut surfaces, store them in sand. During storage, pay attention to ventilation, moisture retention and prevention of root rot. These roots will be used for root propagation. The second section is the stem segment. Cut the stem 30cm above the diameter of the seedling in the seedbed, retain the upper half of the stem segment, and use it for temporary planting. It will be used for propagation by cuttings. The third section consists of seedlings about 30cm long. In the prepared nursery, the seedlings are planted in rows with a spacing of about 40cm between each plant, and are intended to serve as rootstock for grafting.

[0023] For the propagation of *Vernicia fordii* scions, the scion harvesting should begin one week before spring budding, i.e. before grafting. The harvested scions should be completely wrapped in plastic wrap and stored at room temperature for one week to allow proper dehydration before grafting can begin. S3: Tissue Propagation: Propagation of the first section of rhizome: Before spring budding, that is, in early to mid-March 2024, take out the stored root sections, remove the rotten parts, cut them into small root sections of 10-15cm in length, and soak them in mixed solution A for 2-3 hours. Then, arrange them at a spacing of 20cm×30cm, insert the lower part of the root into the prepared nursery ground at a certain angle, cover with fine soil, just enough to bury the root section, gently compact it to ensure close contact between the root section and the soil, and cover with straw or other covering materials, provide appropriate shade, and then carry out seedling management work such as watering, fertilizing, weeding, and pest and disease control in a timely manner. Part Two: Stem Segment Propagation: Before spring budding, in March 2024, take out the stem segments of the transplanted seedlings, select healthy, high-quality stem segments free from pathogen infection, and cut them into small segments of 10-15cm each. Note that each segment should retain 2-3 buds at the top. Cut the lower end of the cutting at an angle and the upper end horizontally. Soak the cuttings in solution B for 2-3 hours, then remove them and dip them in rooting powder solution or disinfectant alcohol. Immediately insert them into the seedbed at 10cm*10cm and compact them. After the cuttings are planted, cover them with a small arched greenhouse with a thin film to keep them warm and moist. Set up a shade net 2 meters above the small arched greenhouse to prevent the temperature from getting too high and to avoid direct sunlight on the cuttings. Then carry out management work such as keeping the cuttings warm and moist, controlling pests and diseases, weeding, and fertilizing. Part Three: Seedling Propagation: In March 2024, when the sap of the rootstock begins to flow, the bark is easy to peel off, and the scion has not yet sprouted, the scion grafting work is carried out. Specifically, in the area where the rootstock of the grafted seedling is planted, the rootstock is cut 5-10cm above the ground. A vertical cut is made on one side of the cross-section of the rootstock, and the scion propagation material of *Vernicia fordii* is taken out. The scion is cut into a small section with 1-2 buds. A long slant is cut on one side of the lower end, and a short slant is cut on the other side. The long slant of the scion is inserted into the cut of the rootstock, so that the cambium layers of the scion and the rootstock are aligned. Then the interface is tied tightly with plastic film. After grafting, check whether it has survived in time, and carry out maintenance and management work such as re-grafting, removing sprouts, cutting, untying, watering, weeding, and fertilizing in a timely manner. S4; Asexual rapid propagation of seedlings for sale: From November 2024 to the end of March 2025, seedlings propagated from the first part of the rhizome and the second part of the stem segment, when the seedling diameter reaches 0.5cm or more and the seedling height reaches 50cm or more, select healthy seedlings free from diseases and pests, and promptly dig up and sell the seedlings during rainy weather, and carry out afforestation work on the mountain. For the third part of the seedlings propagated by grafting, select healthy seedlings with scions that have survived, have well-healed cuts, and are free from diseases and pests, with a grafting diameter of 0.8cm or more and a seedling height of 60cm or more, and prioritize the sale of seedlings for afforestation on the mountain. Furthermore, in step S1, for the selected and prepared seedling propagation materials of *Vernicia fordii*, priority should be given to one-year-old seedlings of *Vernicia fordii* with clear and traceable mother plant characteristics and seed source, and which have been domesticated and cultivated for single-plant propagation in the designated area. Furthermore, in step S2, the pretreatment of propagation materials should be carried out near the nursery of asexually propagated seedlings. During the segmented pretreatment, attention should be paid to checking the preservation of roots, stems, etc. If any rot is found, it should be removed in time. If there is a lack of water, water should be applied in time, but excessive watering should not be done.

[0024] Furthermore, in step S3, during the propagation of the first section of rhizome, the mixed solution A refers to the mixed disinfectant solution of "rooting powder + chlorothalonil", and the ratio used is: 3 grams of 12% indomethacin for rooting agent, 40 grams of chlorothalonil, and 40 jin of water. Furthermore, in step S3, during the propagation of the first rhizome section, the lower part of the root is inserted into the prepared nursery at a certain angle, where a certain angle refers to inserting it at about 45 degrees to increase the contact area between the root and the soil. At the same time, if a bud can be found on the root section, ensure that the bud is retained on the upper soil surface.

[0025] Furthermore, in step S3, during the second part of stem segment propagation, the mixed solution B refers to the mixture of "rooting powder + chlorothalonil + sucrose", and the ratio used is: 3 grams of 12% indomethacin acetic acid rooting agent, 40 grams of chlorothalonil, 5 grams of sucrose, and 40 jin of water. Furthermore, in step S3, during the second part of stem segment propagation, the part dipped in rooting powder solution or disinfectant alcohol is removed. The rooting powder solution is 3 grams of indomethacin diluted in 3 kilograms of water, and the alcohol is 75% medical disinfectant alcohol. The dipping time for each segment should be controlled within 2 seconds. Furthermore, in step S3, during the propagation of seedlings in the third part, the selected scions should preferably be upright buds on the main trunk branches of the mother tree of *Vernicia fordii*. If there are not enough upright buds on the main trunk of *Vernicia fordii*, one-year-old branches of local female *Vernicia fordii* plants that have been bearing fruit continuously, have long and dense fruit clusters, and have a high oil content should be selected as the next best option. Furthermore, in step S3, during the propagation of the seedlings in the third part, the short-cutting work is carried out. This mainly involves observing carefully after the successful grafting of *Vernicia fordii*. Once it is found that the angle between the new branches of *Vernicia fordii* and the rootstock exceeds 10 degrees, the short-cutting work needs to be carried out in time. The short-cutting refers to a method of continuously selecting upright buds on the new branches of the scion, cutting off the excess parts, and cultivating upright buds and upright branches. Furthermore, in step S4, the asexually propagated seedlings should be removed from the nursery in good weather. Attention should be paid to grading and bundling, and they should be planted immediately after being dug up. If they cannot be planted on the same day, they should be temporarily planted to protect their vitality. It is recommended that the time interval between digging up the seedlings and planting should not exceed 15 days.

[0026] At the end of November, using a random selection method, three 1-square-meter quadrats were measured, and the average nursery yields were 96.8%, 95.9%, and 98.2%, respectively, for the first part (rhizome propagation), the second part (stem segment propagation), and the third part (seedling propagation).

[0027] Example 2: The difference from Example 1 is that in step S3, the first section of the rhizome is propagated, and the soaking in the mixed solution A is replaced with soaking in chlorothalonil disinfectant solution. The concentration used is: 40 grams of chlorothalonil diluted in 40 kilograms of water. The difference from Example 1 is that in step S3, the propagation of the first rhizome section is to lay the lower part of the root flat and bury it in the prepared nursery ground. The difference from Example 1 is that in step S3, the second part of the stem segment is propagated, and the soaking in the mixed solution B is replaced with a "rooting powder + chlorothalonil" mixture. The ratio used is: 3 grams of 12% indomethacin and naphthaleneacetic acid rooting agent, 40 grams of chlorothalonil, and 40 jin of water. The difference from Example 1 is that in step S3, the third part of the seedling propagation and the rootstock planted are the rootstocks that will be planted when grafting begins in 2025, that is, the scion grafting work will be carried out as the rootstocks are planted. The difference from Example 1 is that in step S3, the third part of the seedlings is propagated by grafting. After the grafting is propagated, only routine grafted seedling management work such as removing sprouts, weeding, fertilizing, and controlling diseases and pests is carried out. At the end of November, using a random selection method, three 1-square-meter quadrats were measured, and the average nursery yields were 90%, 89%, and 87.9%, respectively, for the first part (rhizome propagation), the second part (stem segment propagation), and the third part (seedling propagation).

[0028] Example 3: The difference from Example 1 is that in step S3, the first section of the rhizome is propagated, and the soaking in the mixed solution A is replaced with soaking in rooting powder. The concentration used is: 3 grams of indole-naphthaleneacetic acid, diluted in 40 catties of water. The difference from Example 1 is that in step S3, the propagation of the first section of rhizome is to bury the lower part of the root at a certain angle, within a range of less than 25 degrees, in the prepared nursery ground; The difference from Example 1 is that in step S3, the second part of the stem segment is propagated, and the soaking in the mixed solution B is replaced with rooting powder solution and carbendazim disinfectant solution, respectively. After soaking for 1 hour, the cuttings are inserted. The rooting powder solution has a concentration of 12% rooting agent, and the carbendazim disinfectant solution has a concentration of 10%. The difference from Example 1 is that in step S3, the third part of the seedling propagation involves selecting one-year-old lateral branches of local female *Vernicia fordii* plants that have been continuously bearing fruit, with long, dense fruit clusters and high oil content. The difference from Example 1 is that in step S3, the third part of the seedlings were propagated without any cutting. At the end of November, using a random selection method, three 1-square-meter quadrats were measured, and the average nursery yields were 86.6%, 85.9%, and 89.7%, respectively, for the first part (rhizome propagation), the second part (stem segment propagation), and the third part (seedling propagation).

[0029] Comparative Example 1: The difference from Example 1 is that in step S3, the first section of the rhizome was propagated without being soaked in the mixed solution A for 2-3 hours; The difference from Example 1 is that in step S3, the propagation of the first rhizome section is to lay the lower part of the root flat and bury it in the prepared nursery ground. The difference from Example 1 is that in step S3, the second part of the stem segment is propagated without being soaked in the mixed solution B for 2-3 hours, and is directly taken out and dipped in rooting powder solution or disinfectant alcohol.

[0030] The difference from Example 1 is that in step S3, the third part of the seedlings were propagated without grafting. The difference from Example 1 is that in step S3, the third part of the seedling propagation only involves routine surface management work such as weeding, fertilization, and pest and disease control. The difference from Example 1 is that in step S3, the third part of the seedling propagation only involves routine seedling work such as weeding, fertilization, and pest and disease control. At the end of November, using a random selection method, three 1-square-meter quadrats were measured, and the average nursery yields for the first part (rhizome propagation), the second part (stem segment propagation), and the third part (seedling propagation) were 50.6%, 43.8%, and 99.5%, respectively.

[0031] By comparison, although Comparative Example 1 carried out asexual propagation in three stages, it did not treat the mother plant seedlings and did not adopt the innovative technology in this invention. As a result, the seedlings had difficulty forming callus tissue, the survival rate was low, and they were susceptible to pests and diseases, leading to root rot and stem rot. In severe cases, the disease could easily spread to seedlings in adjacent nurseries. The cost of manual management was high, the risk of pests and diseases was high, and the yield per unit area was low. It could not achieve the goal of rapid propagation and indirectly increased the risk of seedling cultivation.

[0032] The foregoing description of specific exemplary embodiments of the present invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is obvious that many changes and variations can be made based on the above teachings. Although embodiments of the invention have been shown and described, these specific embodiments are merely explanations of the invention and are not intended to limit it. The specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. The purpose of selecting and describing exemplary embodiments is to explain the specific principles of the invention and its practical application, so that those skilled in the art, after reading this specification, can make modifications, substitutions, variations, and various choices and changes to the embodiments as needed without departing from the principles and spirit of the invention, provided that such modifications, substitutions, variations, and choices and changes are within the scope of the claims of the invention and are protected by patent law.

Claims

1. A method for the asexual and rapid propagation of *Vernicia fordii* seedlings using rhizomes, characterized in that, Includes the following steps: S1: Preparation of propagation materials: In conjunction with the release of seedlings of *Vernicia fordii* this winter and next spring, select healthy, disease-free one-year-old or perennial seedlings of *Vernicia fordii* that have been released from seedlings and have been propagated from seeds of superior varieties and local superior plants, as the mother plant propagation material for the asexual and rapid propagation of *Vernicia fordii* seedlings. S2: Pretreatment of propagation material: Take the rhizome part of the mother plant propagation material of *Vernicia fordii* seedlings, collect the excess roots of the seedlings, collect roots of about 0.5cm-2cm, apply wax to the cut surfaces, and then store them in sand. During storage, pay attention to ventilation, moisture and prevention of root rot, and keep them for use when carrying out root propagation. S3: Tissue propagation: Before spring budding, take out the stored root segments, remove the rotten parts, cut them into small root segments, and soak them in mixed solution A for 2-3 hours. Then, according to the plant spacing of 20cm×30cm, insert the lower part of the root into the prepared nursery ground at a certain angle, cover it with fine soil, just enough to bury the root segment, gently compact it to make the root segment in close contact with the soil, and cover it with straw or other covering materials, provide appropriate shade, and then carry out seedling management work such as watering, fertilizing, weeding, and pest and disease control in a timely manner. S4: Asexual rapid propagation of seedlings for sale: When the seedlings propagated from the rhizomes have a ground diameter of more than 0.5cm and a height of more than 50cm, select healthy seedlings free from diseases and pests, and promptly dig them up and sell them in the nursery during rainy weather, and carry out afforestation work on the mountain.

2. The method for asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes according to claim 1, characterized in that, In step S1, the selected *Vernicia fordii* seedlings have a ground diameter greater than 0.8 cm and a seedling height greater than 1.0 m.

3. The method for asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes according to claim 1, characterized in that, In step S1, for the selected and prepared seedling propagation material of *Vernicia fordii*, one-year-old seedlings of *Vernicia fordii* with clear and traceable mother plant characteristics and seed source are selected.

4. The method for asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes according to claim 1, characterized in that, In step S2, the pretreatment of propagation materials is carried out near the nursery of asexually propagated seedlings. During the segmented pretreatment, the preservation of the rhizomes should be checked at any time. Any rotten parts should be removed in time. Water should be applied in time when water is scarce, but excessive watering should be avoided.

5. The method for asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes according to claim 1, characterized in that, In step S3, the mixture A contains the following components by weight fraction: 3 parts of 12% indomethacin rooting agent, 40 parts of chlorothalonil, and 20,000 parts of water.

6. The method for asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes according to claim 1, characterized in that, In step S3, the length of the small root segment is 10-15 cm.

7. The method for asexual rapid propagation of *Vernicia fordii* seedlings using rhizomes according to claim 1, characterized in that, In step S3, the lower part of the root is inserted into the prepared nursery ground at a certain angle, where a certain angle refers to inserting it at an angle of about 45 degrees.

8. A method for rapid asexual propagation of *Vernicia fordii* seedlings using rhizomes according to claim 7, characterized in that, In step S3, if a bud can be found on the root segment at the lower end, ensure that the bud is retained on the upper soil surface.