Rapid body odor detection reagent and preparation method thereof

Abstract

The invention relates to the technical field of in-vitro diagnosis, in particular to a rapid body odor detection reagent and a preparation method thereof. A rapid body odor detection reagent comprises ninhydrin with the mass percent concentration being 2-4%, a stabilizer with the mass percent concentration being 0.2-25% and a solvent with the volume percent concentration being 82-100%, and the stabilizer is one or more of 2, 6-di-tert-butyl-p-methylphenol, ethylene glycol and vitamin C; the invention discloses a rapid body odor detection reagent and a preparation method thereof, by adding a solvent and a stabilizer into ninhydrin, the stability of the detection reagent is further improved, oxygen can be isolated, hydrolysis can be prevented, and thus the shelf life of a ninhydrin solution is prolonged.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in vitro diagnosis, and in particular to a body odor rapid detection reagent and a preparation method thereof. BACKGROUND

[0002] At present, there is no marketed product for specific detection reagents for human axillary special body odor. In the prior art, the auxiliary diagnosis of the condition of patients with axillary odor mainly relies on the following methods: The first method is a clinical evaluation method based on subjective perception and reference characteristics. This method takes the subjective perception of the special odor of the patient's armpit as the core diagnostic basis, supplemented by family history and oily cerumen as a reference, but lacks clear quantitative diagnostic criteria. In clinical practice, it is usually divided into 5 levels according to the severity of the odor: 1 level shows that there is odor after 5-10 minutes of armpit gauze is taken off; 2 level shows that there is odor immediately after armpit gauze is taken off; 3 level shows that odor can be smelled when clothes are taken off; 4 level shows that odor can be smelled within 1 meter; 5 level shows that odor can be smelled at a distance of more than 1 meter. This method is simple to operate, short in time consumption and does not require additional equipment, but its main defect is that the detection result is easily affected by the subjective judgment difference of the detector.

[0003] The second method is ABCC11 gene single nucleotide polymorphism (SNP) detection, which can assist in judging whether the patient has axillary odor and its severity by detecting the genotype of rs17822931 site of human ABCC11 gene. The advantage of this method is that it can evaluate the degree of disease and genetic possibility of the patient, but its limitation is significant: the detection time is long, the specificity for the environment, living habits and diet of axillary odor formation is insufficient, and it needs to rely on professional and precise instruments and personnel to interpret the results, which is high in cost.

[0004] The third method is immunohistochemical staining detection of axillary tissue skin sections, which can be used as an objective indicator for evaluating the degree of axillary odor by immunohistochemical staining of ABCC11 / MRP8 and ApoD proteins in axillary tissue skin sections. This method has good specificity and sensitivity, but its operation process is complicated, time-consuming, and requires high professional personnel and equipment, which is difficult to popularize clinically.

[0005] In summary, the existing diagnosis and evaluation methods for axillary odor in the prior art either rely on subjective judgment, or have problems such as complicated operation, high cost and poor popularization, and there is still a lack of efficient, objective and easy-to-promote specific detection reagents, so it is urgent to develop a new type of detection reagent to overcome the above defects. SUMMARY

[0006] In view of the deficiencies of the prior art, the present application provides a body odor rapid detection reagent and a preparation method thereof.

[0007] The application adopts the method of chemical reaction. Indantrione can react with amino acids in the armpit swab sample of human to generate a blue-violet compound. The content of amino acids in the armpit sweat of the body odor patient is higher than that of normal people, so the reaction speed with indantrione is fast, and obvious blue or blue-violet color will be presented. If there is no body odor, the indantrione solution will not change color and is light yellow or colorless.

[0008] The application relates to preparation of a protein, amino acid and fatty acid dyeing reagent in an armpit swab sample of a body odor patient or other body odor abnormality patient and application of the reagent in the field of body odor testing.

[0009] In a first aspect, the application provides a body odor rapid detection reagent, which adopts the following technical scheme: A body odor rapid detection reagent comprises 2-4% of indantrione in mass percentage concentration, 0.2-25% of a stabilizer in mass percentage concentration and 82-100% of a solvent in volume percentage concentration. The stabilizer is one or more of 2,6-di-tert-butyl-p-cresol, ethylene glycol and vitamin C.

[0010] By adopting the above technical scheme, the solvent and the stabilizer are added in the application to further improve the stability of the detection reagent because the indantrione solution is easy to oxidize and lose efficacy under normal temperature conditions. The added stabilizer can isolate oxygen and prevent hydrolysis, thereby prolonging the shelf life of the indantrione solution.

[0011] Preferably, the stabilizer is 2,6-di-tert-butyl-p-cresol.

[0012] Further preferably, the mass percentage concentration of the 2,6-di-tert-butyl-p-cresol is 0.2-0.4%.

[0013] By adopting the above technical scheme, the 2,6-di-tert-butyl-p-cresol has an antioxidant effect, can reduce the influence of oxygen on indantrione and can prolong the shelf life. The addition of the stabilizer will not affect the accuracy, sensitivity and specificity of the detection reagent.

[0014] Preferably, the stabilizer is a mixture of 2,6-di-tert-butyl-p-cresol and ethylene glycol.

[0015] Further preferably, the mass percentage concentration of the 2,6-di-tert-butyl-p-cresol is 0.2-0.4%.

[0016] Further preferably, the mass percentage concentration of the ethylene glycol is 15-22.5%.

[0017] By adopting the above technical scheme, 2,6-di-tert-butyl-p-cresol has an antioxidant effect, which can reduce the influence of oxygen on indantrione; ethylene glycol has an anti-hydrolysis effect, which can alleviate the hydrolysis of indantrione; and the addition of the stabilizer does not affect the accuracy, sensitivity and specificity of the detection reagent.

[0018] Preferably, the stabilizer is a mixture of vitamin C and ethylene glycol.

[0019] Further preferably, the mass percentage concentration of vitamin C is 0.5-1.5%.

[0020] Further preferably, the mass percentage concentration of ethylene glycol is 15-22.5%.

[0021] By adopting the above technical scheme, vitamin C has an antioxidant effect, which can reduce the influence of oxygen on indantrione; ethylene glycol has an anti-hydrolysis effect, which can alleviate the hydrolysis of indantrione; and the addition of the stabilizer does not affect the accuracy, sensitivity and specificity of the detection reagent.

[0022] Preferably, the solvent is ethanol.

[0023] In a second aspect, the application provides a preparation method of a body odor rapid detection reagent, which adopts the following technical scheme: A preparation method of a body odor rapid detection reagent, the preparation method steps are as follows: According to the formula, weigh each raw material; Mix indantrione and solvent under light-proof conditions, then add a stabilizer and mix to obtain a detection reagent.

[0024] Preferably, the temperature for mixing indantrione and solvent is 20-30℃.

[0025] Preferably, the temperature for mixing indantrione and solvent is 20-30℃.

[0026] Preferably, the rotation speed for mixing indantrione and solvent is 35-55rpm, and the mixing time is 25-35min.

[0027] Preferably, the rotation speed for mixing indantrione and solvent is 35-55rpm, and the mixing time is 25-35min.

[0028] In summary, the application has the following at least one beneficial technical effect: 1. The application discloses a body odor rapid detection reagent and a preparation method thereof, which further improves the stability of the detection reagent by adding a solvent and a stabilizer to the detection reagent; 2. The application discloses a body odor rapid detection reagent and a preparation method thereof. In the application, a stabilizer is added to isolate oxygen and prevent hydrolysis, thereby prolonging the shelf life of the indantrione solution. 3. The detection reagent is simple to operate, time-saving, low in cost, and can be easily detected by professionals or non-professionals. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 is a colorimetric card detection result picture. DETAILED DESCRIPTION

[0030] The technical solutions of the application are further illustrated by specific examples below, and the specific examples do not represent a limitation on the protection scope of the application; some non-essential modifications and adjustments made by others according to the concept of the application still belong to the protection scope of the application.

[0031] The raw materials involved in the application all use commercially available products, and the application is further described in detail below in combination with examples and comparative examples.

[0032] A body odor rapid detection reagent comprises 2-4% of indantrione in terms of mass percentage concentration, 0.2-25% of a stabilizer in terms of mass percentage concentration, and 82-100% of a solvent in terms of volume percentage concentration. The stabilizer is one or more of 2,6-di-tert-butyl-p-cresol, ethylene glycol and vitamin C.

[0033] Preferably, the stabilizer is 2,6-di-tert-butyl-p-cresol. Further preferably, the mass percentage concentration of the 2,6-di-tert-butyl-p-cresol is 0.2-0.4%, for example, 0.2%, 0.3% or 0.4%.

[0034] In a specific implementable scheme, the mass percentage concentration of the 2,6-di-tert-butyl-p-cresol is 0.3%.

[0035] Preferably, the stabilizer is a mixture of 2,6-di-tert-butyl-p-cresol and ethylene glycol.

[0036] Further preferably, the mass percentage concentration of the 2,6-di-tert-butyl-p-cresol is 0.2-0.4%, for example, 0.2%, 0.3% or 0.4%.

[0037] Further preferably, the mass percentage concentration of the ethylene glycol is 15-22.5%, for example, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22% or 22.5%.

[0038] In a specific embodiment, the 2,6-di-tert-butyl-p-cresol has a mass percentage concentration of 0.3%; and the ethylene glycol has a mass percentage concentration of 18%.

[0039] Preferably, the stabilizer is a mixture of vitamin C and ethylene glycol.

[0040] Further preferably, the vitamin C has a mass percentage concentration of 0.5-1.5%; for example, a mass percentage concentration of 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, or 1.5%.

[0041] Further preferably, the ethylene glycol has a mass percentage concentration of 15-22.5%; for example, a mass percentage concentration of 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, or 22.5%.

[0042] In a specific embodiment, the vitamin C has a mass percentage concentration of 1%; and the ethylene glycol has a mass percentage concentration of 18%.

[0043] Preferably, the solvent is ethanol.

[0044] In a specific embodiment, the solvent has a volume percentage concentration of 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

[0045] A method for preparing the body odor rapid detection reagent, comprising the following steps: According to the formula, each raw material is weighed; Under light-proof conditions, the indanetrione and the solvent are mixed, and then the stabilizer is added and mixed to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0046] The detection reagent is divided into 2 mL flat-bottom screw tubes at a loading of 500 μL per tube, and after the division is completed, it should be immediately packed into a packaging box or temporarily stored in an aluminum foil bag to prevent light from affecting the stability of the reagent.

[0047] Using such a screw tube can ensure that the reagent will not leak, and when detection is performed, it can be directly placed vertically on a plane without the need for any support, facilitating observation.

[0048] Preferably, the temperature for mixing the indanetrione and the solvent is 20-30°C.

[0049] Preferably, the temperature for mixing the indanetrione and the solvent is 20-30°C.

[0050] Preferably, the rotation speed of mixing the indantrione and the solvent is 35-55 rpm, and the mixing time is 25-35 min.

[0051] Preferably, the rotation speed of mixing the stabilizer is 35-55 rpm, and the mixing time is 25-35 min.

[0052] Example 1:

[0053] A preparation method of a body odor rapid detection reagent, comprising the following steps: Under the light-proof condition, take one 125 mL capacity brown wide-mouth reagent bottle, add 100 mL of anhydrous ethanol, weigh 2 g of indantrione, slowly add it into the anhydrous ethanol, place it on a shaker, shake at 45 rpm at 25°C for 30 min until complete dissolution, then weigh 0.2 g of 2,6-di-tert-butyl-p-cresol, add it into the solution, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until complete dissolution, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0054] Example 2:

[0055] A preparation method of a body odor rapid detection reagent, comprising the following steps: Under the light-proof condition, take one 125 mL capacity brown wide-mouth reagent bottle, add 100 mL of anhydrous ethanol, weigh 3 g of indantrione, slowly add it into the anhydrous ethanol, place it on a shaker, shake at 45 rpm at 25°C for 30 min until complete dissolution, then weigh 0.3 g of 2,6-di-tert-butyl-p-cresol, add it into the solution, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until complete dissolution, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0056] Example 3:

[0057] A preparation method of a body odor rapid detection reagent, comprising the following steps: Under the light-proof condition, take one 125 mL capacity brown wide-mouth reagent bottle, add 100 mL of anhydrous ethanol, weigh 4 g of indantrione, slowly add it into the anhydrous ethanol, place it on a shaker, shake at 45 rpm at 25°C for 30 min until complete dissolution. Then weigh 0.4 g of 2,6-di-tert-butyl-p-cresol, add it into the solution, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until complete dissolution, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0058] Example 4:

[0059] The difference from Example 2 is that the amount of 2,6-di-tert-butyl-p-cresol added is 0.2 g.

[0060] Example 5:

[0061] The difference from Example 2 is that the amount of 2,6-di-tert-butyl-p-cresol added is 0.4 g.

[0062] Example 6:

[0063] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of avoiding light, take 1 piece of 125 mL capacity brown wide-mouth reagent bottle, add 88 mL of anhydrous ethanol; weigh 2 g of indanetrione, slowly add it to the anhydrous ethanol, and place it on a shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved; then weigh 0.2 g of 2,6-di-tert-butyl-p-cresol, add it to the solution, and then add 15 g of ethylene glycol, after the addition is completed, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0064] Example 7:

[0065] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of avoiding light, take 1 piece of 125 mL capacity brown wide-mouth reagent bottle, add 85 mL of anhydrous ethanol; weigh 3 g of indanetrione, slowly add it to the anhydrous ethanol, and place it on a shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved; then weigh 0.3 g of 2,6-di-tert-butyl-p-cresol, add it to the solution, and then add 18 g of ethylene glycol, after the addition is completed, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0066] Example 8:

[0067] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of avoiding light, take 1 brown reagent bottle with a capacity of 125 mL, add 82 mL of anhydrous ethanol; weigh 4 g of indanetrione, slowly add it to the anhydrous ethanol, and place it on a shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved; then weigh 0.4 g of 2,6-di-tert-butyl-p-cresol, add it to the solution, and then add 22.5 g of ethylene glycol, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0068] Example 9:

[0069] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of avoiding light, take 1 brown reagent bottle with a capacity of 125 mL, add 88 mL of anhydrous ethanol; weigh 2 g of indanetrione, slowly add it to the anhydrous ethanol, and place it on a shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved; then weigh 0.5 g of vitamin C (i.e. L-ascorbic acid), add it to the solution, and then add 15 g of ethylene glycol, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0070] Example 10:

[0071] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of avoiding light, take 1 brown reagent bottle with a capacity of 125 mL, add 85 mL of anhydrous ethanol; weigh 3 g of indanetrione, slowly add it to the anhydrous ethanol, and place it on a shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved; then weigh 1 g of vitamin C (i.e. L-ascorbic acid), add it to the solution, and then add 18 g of ethylene glycol, continue to place it on the shaker, shake at 45 rpm at 25°C for 30 min until completely dissolved, to obtain a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0072] Example 11:

[0073] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of avoiding light, take 1 125mL capacity of brown reagent bottle, add 82mL of anhydrous ethanol; 4g of indanone is weighed and slowly added to the anhydrous ethanol, placed on the shaking table, shaken at 45rpm at 25℃ for 30min until completely dissolved; then 1.5g of vitamin C (i.e. L-ascorbic acid) is weighed and added to the solution, then 22.5g of ethylene glycol is added, after the addition is completed, continue to be placed on the shaking table, shaken at 45rpm at 25℃ for 30min until completely dissolved, to obtain a clear colorless to light yellow liquid, which is the detection reagent.

[0074] Example 12: The difference from example 2 is that the temperature of mixing is different.

[0075] In this embodiment, the specific steps are: placed on the shaking table, shaken at 45rpm at 20℃ for 30min until completely dissolved; continue to be placed on the shaking table, shaken at 45rpm at 20℃ for 30min until completely dissolved.

[0076] Example 13:

[0077] The difference from example 2 is that the temperature of mixing is different.

[0078] In this embodiment, the specific steps are: placed on the shaking table, shaken at 45rpm at 30℃ for 30min until completely dissolved; continue to be placed on the shaking table, shaken at 45rpm at 30℃ for 30min until completely dissolved.

[0079] Comparative example 1:

[0080] A preparation method of a body odor rapid detection reagent, the steps are as follows: Take 1 125mL capacity of brown reagent bottle, add 100mL of anhydrous ethanol, weigh 3g of indanone and slowly add to the anhydrous ethanol, place on the shaking table, shake at 45rpm at 25℃ for 30min until completely dissolved. Then 0.3g of 2,6-di-tert-butyl-p-methylphenol is weighed and added to the solution, continue to be placed on the shaking table, shaken at 45rpm at 25℃ for 30min until completely dissolved, to obtain the detection reagent.

[0081] Comparative example 2:

[0082] The difference from example 2 is that the temperature of mixing is different.

[0083] In this embodiment, the specific steps are: placed on the shaking table, shaken at 45rpm at 19℃ for 30min until completely dissolved; continue to be placed on the shaking table, shaken at 45rpm at 19℃ for 30min until completely dissolved.

[0084] Comparative Example 3:

[0085] The difference from Example 2 is that the temperature of mixing is different.

[0086] In this example, the specific steps are as follows: place on a shaker, shake at 45 rpm for 30 min at 31°C until completely dissolved; continue to place on a shaker, shake at 45 rpm for 30 min at 31°C until completely dissolved.

[0087] Comparative Example 4:

[0088] The difference from Example 2 is that the amount of 2,6-di-tert-butyl-p-cresol added is 0.1 g.

[0089] Comparative Example 5:

[0090] The difference from Example 2 is that the amount of 2,6-di-tert-butyl-p-cresol added is 0.5 g.

[0091] Comparative Example 6:

[0092] A preparation method of a body odor rapid detection reagent, the steps are as follows: Under the condition of light protection, take a 125 mL capacity brown reagent bottle, add 100 mL of anhydrous ethanol, 3 g of indanetrione, and 0.3 g of 2,6-di-tert-butyl-p-cresol, after adding, place on a shaker, shake at 45 rpm for 30 min at 25°C until completely dissolved, get a transparent and clear colorless to light yellow liquid, which is the detection reagent.

[0093] Performance detection:

[0094] 1. Accuracy detection Sampling method: The sample for detection is a human armpit swab sample, and the tester should avoid being in a sweating state during sampling, as sweat can affect the results. When sampling, place the head of the polyester swab or absorbent cotton swab in the armpit, and the patient should pinch the swab and rotate it 3-5 times clockwise.

[0095] Detection method: After sampling, detection should be carried out immediately, place the swab with the sample in the detection reagent prepared in Examples 1-13, ensure that the swab head is immersed in the solution, then rotate 3-5 times, and observe the results after 1 minute; if the reagent turns blue or blue-purple, it is positive, if it does not change color, it is negative, the detection standard is shown in Figure 1 .

[0096] Sample test: 7 samples of axillary odor positive patients and 10 samples of axillary odor negative persons were taken and tested according to the above sampling and testing procedures. The personnel were in a calm state and the axillary region did not sweat a lot when sampling. The test was performed immediately after sampling. The coincidence rate of the reagent was determined by taking the patient's clinical symptoms as the gold standard.

[0097] Table 1 Accuracy test results

[0098] Results: 7 samples with positive clinical symptoms, the results of the body odor test reagent were all positive, the positive coincidence rate was 100.00%; 10 samples with negative clinical symptoms, the results of the body odor test reagent were 9 negative and 1 positive, the negative coincidence rate was 90.00%, among which, the detection result of No. 2 negative sample was false positive due to the presence of sweat in the axillary region during sampling. The person had no symptoms of axillary odor, so compared with the clinical symptoms, the negative coincidence rate of the body odor test reagent was 100.00%. The results meet the expectations.

[0099] 2. Sensitivity test results The sensitivity of the test reagent samples prepared in the above examples and comparative examples was detected. The mass concentration of 3% BSA and the mass concentration of 2% BSA were used as reference, 20 μL of mass concentration of 3% BSA and 20 μL of mass concentration of 2% BSA were added to the test reagent sample, and then the sample of the axillary odor positive patient was added. The color change of the test reagent was observed and the change time was recorded.

[0100] Table 2 Sensitivity test results

[0101] As shown in Table 2, the test reagent prepared in the present application has good sensitivity, which shows that the addition of the stabilizer does not affect the sensitivity of the test reagent.

[0102] 3. Specificity test results 100 samples of axillary odor negative persons and 50 samples of axillary odor positive patients were collected, and the test reagent samples prepared in the above examples and comparative examples were used to detect the samples, and the detection results are shown in the following table.

[0103] Table 3 Specificity test results

[0104] As shown in Table 3, the test reagent prepared in the present application has good specificity, which shows that the addition of the stabilizer does not affect the specificity of the test reagent; and when the stabilizer is 2,6-di-tert-butyl-p-cresol, the color change of the test reagent is more obvious and the detection effect is better.

[0105] 4. Stability The stability of the test reagent samples prepared in the above examples and comparative examples was detected, and the specific steps were as follows: The test reagent samples were placed at 25°C for 0 days, 3 months, 6 months, 9 months, 12 months, and 15 months, respectively, and the appearance of the test reagent samples and the detection ability of the test reagent were detected.

[0106] The indanone sample was placed at 25°C for 0 days, 1 month, 3 months, and 6 months, respectively, and the appearance of the test reagent sample and the detection ability of the test reagent were detected.

[0107] Table 4: Stability detection results

[0108]

[0109]

[0110] From the detection results in Table 4, it can be seen that the test reagents prepared in Examples 1-13 meet the requirements in terms of various performance indicators within 12 months.

[0111] From the detection results of Examples 2 and Comparative Example 1, it can be seen that the stability detection results of Example 2 are better than those of Comparative Example 1, indicating that preparing the test reagent under light-proof conditions can improve the stability of indanone, thereby further improving the stability of the test reagent.

[0112] From the detection results of Examples 2, 12, 13, Comparative Example 2, and Comparative Example 3, it can be seen that the temperature of mixing during preparation of the test reagent has a certain effect on the stability of the test reagent. When the mixing temperature is low, the raw materials are not completely dissolved, thereby resulting in poor stability of the prepared test reagent. When the mixing temperature is high, it affects the stability of indanone, thereby resulting in poor stability of the prepared test reagent. And when the mixing temperature is 20-30°C, the stability of the prepared test reagent is best.

[0113] From the detection results of Examples 2, 4, 5, Comparative Example 4, and Comparative Example 5, it can be seen that the amount of stabilizer added has a certain effect on the stability of the test reagent, and when the stabilizer is 2,6-di-tert-butyl-p-cresol and the amount added is 0.2-0.4 g, the stability of the prepared test reagent is best.

[0114] It can be seen from the detection results of example 2 and comparative example 6 that the stability detection results of example 2 are better than those of comparative example 6, indicating that the addition sequence of raw materials has an influence on the stability of the detection reagent. In the present application, the indantrione is first dissolved in a solvent, and then mixed with the stabilizer, so that the raw materials can be completely dissolved, thereby further improving the stability of the detection reagent.

[0115] The present application discloses a kind of body odor rapid detection reagent and preparation method thereof, extremely short time consumption, without gene detection and immunohistochemical long time waiting;Better specificity;Higher sensitivity;Low cost, without gene detection high cost;Simple operation, without professional personnel, professional or non-professional personnel can easily detect;Without professional instrument, compared with gene detection and immunohistochemical method, it is easier to develop.

Claims

1. A rapid body odor detection reagent, characterized in that: It includes ninhydrin at a mass percentage concentration of 2-4%, a stabilizer at a mass percentage concentration of 0.2-25%, and a solvent at a volume percentage concentration of 82-100%. The stabilizer is one or more of 2,6-di-tert-butyl-p-methylphenol, ethylene glycol, and vitamin C.

2. The rapid body odor detection reagent according to claim 1, characterized in that: The stabilizer is 2,6-di-tert-butyl-p-methylphenol; the mass percentage concentration of the 2,6-di-tert-butyl-p-methylphenol is 0.2-0.4%.

3. The rapid body odor detection reagent according to claim 1, characterized in that: The stabilizer is a mixture of 2,6-di-tert-butyl-p-methylphenol and ethylene glycol; the mass percentage concentration of the 2,6-di-tert-butyl-p-methylphenol is 0.2-0.4%, and the mass percentage concentration of the ethylene glycol is 15-22.5%.

4. The rapid body odor detection reagent according to claim 1, characterized in that: The stabilizer is a mixture of vitamin C and ethylene glycol; the mass percentage concentration of vitamin C is 0.5-1.5%; and the mass percentage concentration of ethylene glycol is 15-22.5%.

5. The rapid body odor detection reagent according to claim 1, characterized in that: The solvent is ethanol.

6. A method for preparing the rapid body odor detection reagent according to any one of claims 1-5, characterized in that, The preparation method steps are as follows: Weigh out each ingredient according to the formula; Under light-protected conditions, ninhydrin and solvent are mixed, and then a stabilizer is added and mixed again to obtain the detection reagent.

7. The preparation method according to claim 6, characterized in that: The temperature at which ninhydrin and the solvent are mixed is 20-30℃.

8. The preparation method according to claim 6, characterized in that: The temperature for mixing with the stabilizer is 20-30℃.

9. The preparation method according to claim 6, characterized in that: The ninhydrin and solvent are mixed at a speed of 35-55 rpm for a time of 25-35 min.

10. The preparation method according to claim 6, characterized in that: When adding the stabilizer, the mixing speed is 35-55 rpm and the mixing time is 25-35 min.

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