Preparation method of analgesic hydrogel dressing based on fritillaria alkaloid

Abstract

The invention discloses a preparation method of an analgesic hydrogel dressing based on fritillaria alkaloid, and belongs to the technical field of biomedical dressing preparation, and the preparation method comprises the following steps: dispersing fritillaria alkaloid in a hydrogel matrix material, and carrying out physical crosslinking or chemical crosslinking to obtain the analgesic hydrogel dressing based on fritillaria alkaloid. The core of the invention lies in that fritillaria alkaloid with natural analgesic activity is combined with the traditional polymer hydrogel, and sustained release of fritillaria alkaloid on the wound surface is realized by virtue of the slow release characteristic of the hydrogel, so that the pain experience of a patient in the wound healing process is effectively relieved. Meanwhile, the hydrogel matrix has good moisture retention, biocompatibility and healing promotion characteristics, and an ideal microenvironment can be provided for tissue repair. The pain feeling in the wound repairing period is remarkably reduced, the wound healing process is accelerated, the overall comfort level and life quality of a patient are improved, and wide clinical application prospects are achieved.

Description

Technical Field

[0001] This invention belongs to the field of biomedical dressing preparation technology, and more specifically, this invention relates to a method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids. Background Technology

[0002] Wound repair materials have wide applications in clinical treatment, playing a crucial role, especially in postoperative wound management, chronic ulcer care, and burn care. Currently commonly used dressing materials include non-woven fabrics, foams, alginate, and hydrogels. Among these, hydrogels, due to their excellent moisturizing properties, softness, and biocompatibility, can provide a moist and oxygen-permeable repair environment for wounded tissues, significantly promoting cell proliferation and tissue regeneration, and have become an important research direction in the field of wound care.

[0003] However, existing hydrogel dressings still have significant limitations in relieving traumatic pain. Wound pain not only affects patients' quality of life but may also reduce their treatment adherence and prolong the recovery period. Therefore, developing novel hydrogel materials that combine analgesic and reparative functions has become an urgent technical problem to be solved.

[0004] Traditional Chinese medicine resources have demonstrated unique advantages in wound treatment. Fritillaria cirrhosa is a traditional Chinese medicine commonly used for its antitussive, expectorant, and anti-inflammatory properties. Its extracts are rich in various bioactive alkaloids, such as phenacidine and fetropine, which exhibit clear analgesic, anti-inflammatory, and antibacterial activities. Although studies have demonstrated its pharmacological potential, the technology for effectively loading Fritillaria cirrhosa alkaloids into hydrogel systems for sustained release and wound treatment is still immature.

[0005] Therefore, there is an urgent need for an innovative composite material that combines the active ingredients of traditional Chinese medicine with modern hydrogel technology to achieve a synergistic effect of analgesia and wound repair, thereby meeting the urgent clinical demand for safe, efficient and comfortable dressing materials. Summary of the Invention

[0006] One object of the present invention is to solve at least the above-mentioned problems and / or defects, and to provide at least the advantages described below.

[0007] To achieve these and other advantages according to the present invention, the present invention provides a method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids, specifically comprising the following steps: Step 1: Prepare a fritillary alkaloid solution with a concentration of 0.001wt%~0.1wt% using fritillary alkaloids; Step 2: Mix the fritillaria alkaloid solution with the hydrogel matrix material precursor solution to obtain a composite solution; add 5%~15% (by mass of the hydrogel matrix material) of modified polyacrylic acid to the composite solution; wherein, the preparation method of the modified polyacrylic acid includes: S21. Hydroxylated alumina nanoparticles are then modified with 3-(2,3-epoxypropoxy)propyltrimethoxysilane to obtain modified alumina nanoparticle powder. The specific method includes: adding alumina nanoparticle powder with a particle size of 20-60 nm into a 2-5 wt% H2O2 solution, stirring at 1200-2000 rpm for 12-24 h, and letting it stand for 8-12 h; adding 3-(2,3-epoxypropoxy)propyltrimethoxysilane to the system, stirring at 200-600 rpm and heating to 80-100℃ for 6-24 h, cooling and letting it stand, centrifuging to separate the solid and liquid phases, washing the solid powder multiple times, and vacuum drying at 50-80℃ to obtain hydroxylated alumina nanoparticles. S22. Disperse hydroxylated nano-alumina in a 10-20 wt% polyacrylic acid solution, using polyethylene glycol-200 as a dispersant, with an ultrasonic dispersion frequency of 40-80 kHz and a dispersion time of 1-2 h. After standing for 12-24 h, evaporate the water at 80-100 °C to obtain modified polyacrylic acid. Step 3: Add a cross-linking agent to the composite solution to form a three-dimensional network hydrogel structure by chemical cross-linking or physical cross-linking, thereby obtaining analgesic hydrogel dressing based on fritillaria alkaloids.

[0008] Preferably, in step one, the fritillaria alkaloids include one or more of isosteroidal alkaloids, steroidal alkaloids, fritillaria oxalis, fritillaria oxalis, and fritillaria oxalis; wherein the isosteroidal alkaloids include fritillaria oxalis, fritillaria oxalis A, fritillaria oxalis B, fritillaria oxalis, fritillaria oxalis, fritillaria oxalis, and fritillaria oxalis ketone.

[0009] Preferably, in step two, the concentration of the hydrogel matrix material in the hydrogel matrix material precursor solution is 0.5wt~50wt.

[0010] Preferably, in step two, the hydrogel matrix material in the hydrogel matrix material precursor solution is one or more of chitosan, gelatin, chitin, cellulose, glucomannan, sodium alginate, polyacrylic acid, polyacrylamide, or hyaluronic acid.

[0011] Preferably, in step two, the fritillaria alkaloids in the hydrogel matrix material precursor solution are added in the form of solution, alkaloid powder, or alkaloid dispersion. The amount of Fritillaria alkaloid added to the precursor solution of the hydrogel matrix material is 0.01% to 5% of the mass of the hydrogel matrix material.

[0012] Preferably, in step S21, the ratio of nano-alumina powder to H2O2 solution is 1~5:10~20mL:1~10mL.

[0013] Preferably, in step S22, the ratio of polyacrylic acid solution, hydroxylated nano-alumina, and polyethylene glycol-200 is 100~250mL:1~5g:0.1~1g.

[0014] Preferably, in step three, the crosslinking agent is added in the form of a crosslinking agent solution, the concentration of the crosslinking agent solution is 0~10wt%, the volume of the crosslinking agent solution is 0~10% of the volume of the fritillaria alkaloid solution, and after the crosslinking agent is added, the crosslinking reaction includes one of freeze-thaw cycle and ultraviolet irradiation.

[0015] Preferably, in step three, the physical crosslinking method includes inducing crystallization or enhancing hydrogen bond interactions; the chemical crosslinking method includes adding a crosslinking agent, which includes one or more of glutaraldehyde, genipin, and epichlorohydrin.

[0016] Preferably, the analgesic hydrogel dressing has a water content of 50% to 99.5%.

[0017] The present invention has at least the following beneficial effects: (1) The analgesic hydrogel dressing based on Fritillaria alkaloids provided by the present invention not only has excellent wound repair performance, but also enhances the activity of inhibitory neurotransmitters (such as γ-aminobutyric acid, GABA) through the sustained release effect of alkaloids, and reduces the excitability of the central nervous system; at the same time, it blocks the nuclear factor κB (NF-κB) signaling pathway, effectively relieving inflammatory (such as arthritis) and neuropathic pain (such as cancer pain), thereby significantly reducing the patient's pain while promoting wound healing.

[0018] (2) The fritillaria alkaloids used have good biocompatibility with the hydrogel matrix, have no adverse effects on human metabolism during use, are highly safe, and are suitable for long-term or sensitive people.

[0019] (3) The hydrogel matrix material used in this invention is a polymer network structure with sustained-release properties. Fritillaria alkaloids can be stably embedded in this structure and continuously released, thereby producing analgesic and healing effects on the wound site.

[0020] (4) The analgesic hydrogel dressing based on fritillaria alkaloids prepared in this invention not only has analgesic and healing-promoting effects on wound sites, but also has excellent mechanical properties. By adding hydroxylated nano-alumina powder to polyacrylic acid, the compatibility between the hydroxylated and coupled-modified nano-alumina powder and polyacrylic acid is improved. Polyethylene glycol-200 is used as a dispersant to improve the dispersion performance of the hydroxylated nano-alumina powder in polyacrylic acid solution. Among them, the hydroxylation treatment of nano-alumina with H2O2 increases the hydroxyl density on the surface of nano-alumina powder, providing a large number of reactive sites for subsequent reaction with 3-(2,3-epoxypropoxy)propyltrimethoxysilane; and the two end groups of 3-(2,3-epoxypropoxy)propyltrimethoxysilane (KH-560) are methoxy (-OCH3) and epoxy group, respectively. The methoxy group of KH-560 hydrolyzes in an aqueous environment to generate highly reactive silanol (-SiOH). These silanols undergo dehydration condensation with the -OH groups on the surface of hydroxylated nano-alumina to form strong Al-O-Si covalent bonds. This KH-560 is firmly anchored to the surface of the alumina particles, giving the inorganic nano-alumina powder a long-chain, epoxy-group-containing organically compatible surface. This improves the compatibility between hydroxylated nano-alumina and polyacrylic acid, laying the foundation for improving the mechanical properties of analgesic hydrogel dressings based on fritillaria alkaloids. Subsequently, the hydroxylated alumina powder and polyacrylic acid solution are covalently cross-linked to form a three-dimensional cross-linked network, thereby improving the modulus, strength, and toughness of polyacrylic acid. Adding modified polyacrylic acid to the mixed solution can significantly improve the tensile strength and yield strength of analgesic hydrogel dressings based on fritillaria alkaloids.

[0021] Other advantages, objectives and features of the present invention will become apparent in part from the following description, and in part from those skilled in the art through study and practice of the invention. Attached Figure Description

[0022] Figure 1 Photograph of the analgesic hydrogel dressing based on fritillaria alkaloids prepared in Example 1; Figure 2 Cell compatibility evaluation of the analgesic hydrogel dressing based on fritillaria alkaloids prepared in Example 1; Figure 3 Evaluation of the wound repair performance of the analgesic hydrogel dressing based on fritillaria alkaloids prepared in Example 1; Figure 4 Histological section photograph of the analgesic hydrogel dressing based on fritillaria alkaloids prepared in Example 1. Detailed Implementation

[0024] The present invention will now be described in further detail with reference to the accompanying drawings, so that those skilled in the art can implement it based on the description.

[0025] It should be understood that terms such as “having,” “comprising,” and “including” as used herein do not exclude the presence or addition of one or more other elements or combinations thereof. Example 1 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0026] Step 2: Add 5 mL of 5 wt% glutaraldehyde solution to the above mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10 min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0027] The analgesic hydrogel dressing based on fritillaria alkaloids prepared in this embodiment is shown in the image below. Figure 1 As shown.

[0028] Figure 2 During the cell compatibility experiment of the analgesic hydrogel dressing based on fritillaria alkaloids prepared in Example 1, the adhesion photographs of cells on the surface of the analgesic hydrogel dressing based on fritillaria alkaloids show that cells only adhere to the surface of materials with good biocompatibility. Figure 2 It can be found that cells have good adhesion properties on the surface of the analgesic hydrogel dressing based on fritillaria alkaloids, indicating that the analgesic hydrogel dressing based on fritillaria alkaloids has excellent cell compatibility and can be used as a hydrogel-based dressing.

[0029] Figure 3 The images show the repair process after the analgesic hydrogel dressing based on fritillaria alkaloids prepared in this embodiment was implanted into mice. The top image shows the analgesic hydrogel dressing, and the bottom image shows the blank control group. The comparison reveals that the wounds in the group with the hydrogel dressing closed almost completely, while the repair efficiency in the group without the hydrogel dressing was low.

[0030] Figure 4 Histological sections of the fully repaired wound revealed significant collagen deposition after treatment with the Fritillaria alkaloid-based analgesic hydrogel dressing, indicating high tissue maturity in the mice. The absence of numerous inflammatory cells demonstrates the good biocompatibility of our wound dressing.

[0031] Example 2 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0032] Step 2: Transfer the above mixed solution to a 4°C environment and keep it warm for 15 minutes to obtain analgesic hydrogel dressing based on fritillaria alkaloids.

[0033] Example 3 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillariaein A and 0.05g of fritillariaein B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of chitosan powder in 100mL of deionized water, and add 2mL of glacial acetic acid until completely dissolved. Mix the fritillaria alkaloid solution with the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0034] Step 2: Add 5 mL of 5 wt% glutaraldehyde solution to the above mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10 min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0035] Example 4 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillariaein A and 0.05g of fritillariaein B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of chitosan powder in 100mL of deionized water, and add 2mL of glacial acetic acid until completely dissolved. Mix the fritillaria alkaloid solution with the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0036] Step 2: Add 5 mL of 5 wt% genipin solution to the above mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10 min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0037] Example 5 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0038] Step 2: Add 0.5g of modified polyacrylic acid to the mixed solution, and simultaneously add 5mL of 5wt% glutaraldehyde solution to the mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0039] The preparation methods for modified polyacrylic acid include: S21. Add 25g of nano-alumina powder with a particle size of 20~60nm to 200mL of 2wt% H2O2 solution, stir at 1200rpm for 12h, and let stand for 12h; add 10mL of 3-(2,3-epoxypropoxy)propyltrimethoxysilane to the system, stir at 300rpm and heat to 90℃ for 12h, cool and let stand, centrifuge to separate solid and liquid, wash the solid powder several times with deionized water, and vacuum dry at 80℃ to obtain hydroxylated nano-alumina; S22. Disperse 2g of hydroxylated nano-alumina into 250mL of a 20wt% polyacrylic acid solution, using 0.5g of polyethylene glycol-200 as a dispersant. The ultrasonic dispersion frequency is 80kHz, the dispersion time is 1h, and after standing for 24h, the water is removed by evaporation at 80℃ to obtain modified polyacrylic acid.

[0040] Example 6 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0041] Step 2: Add 0.5g of modified polyacrylic acid to the mixed solution, and simultaneously add 5mL of 5wt% glutaraldehyde solution to the mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0042] The preparation methods for modified polyacrylic acid include: S21. Add 25g of nano-alumina powder with a particle size of 20~60nm to 200mL of 2wt% H2O2 solution, stir at 1200rpm for 24h, let stand for 12h, add 10mL of 3-(2,3-epoxypropoxy)propyltrimethoxysilane to the system, stir at 300rpm and heat to 90℃ for 12h, cool and let stand, centrifuge to separate solid and liquid, wash the solid powder with deionized water several times, and vacuum dry at 80℃ to obtain hydroxylated nano-alumina. S22. Disperse 4g of hydroxylated nano-alumina into a 20wt% polyacrylic acid solution, using 0.5g of polyethylene glycol-200 as a dispersant. The ultrasonic dispersion frequency is 60kHz, the dispersion time is 1h, and after standing for 24h, the water is removed by evaporation at 80℃ to obtain modified polyacrylic acid.

[0043] Example 7 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0044] Step 2: Add 0.5g of modified polyacrylic acid to the mixed solution, and simultaneously add 5mL of 5wt% glutaraldehyde solution to the mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0045] The preparation methods for modified polyacrylic acid include: S21. Add 25g of nano-alumina powder with a particle size of 20~60nm to 200mL of 3wt% H2O2 solution, stir at 1200rpm for 24h, let stand for 12h, add 10mL of 3-(2,3-epoxypropoxy)propyltrimethoxysilane to the system, stir at 300rpm and heat to 90℃ for 12h, cool and let stand, centrifuge to separate solid and liquid, wash the solid powder with deionized water several times, and vacuum dry at 80℃ to obtain hydroxylated nano-alumina. S22. Disperse 5g of hydroxylated nano-alumina into a 20wt% polyacrylic acid solution, using 0.6g of polyethylene glycol-200 as a dispersant. The ultrasonic dispersion frequency is 60kHz, the dispersion time is 1h, and after standing for 24h, the water is removed by evaporation at 80℃ to obtain modified polyacrylic acid.

[0046] Comparative Example 1 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0047] Step 2: Add 0.5g of modified polyacrylic acid to the mixed solution, and simultaneously add 5mL of 5wt% glutaraldehyde solution to the mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0048] The preparation methods for modified polyacrylic acid include: 2g of nano-alumina was dispersed in 250mL of a 20wt% polyacrylic acid solution. 0.5g of polyethylene glycol-200 was used as a dispersant. The ultrasonic dispersion frequency was 80kHz and the dispersion time was 1h. After standing for 24h, the water was removed by evaporation at 80℃ to obtain modified polyacrylic acid.

[0049] Comparative Example 2 A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids includes the following steps: Step 1: Weigh 0.1g of fritillaria alkaloids (including 0.05g of fritillarin A and 0.05g of fritillarin B) and dissolve them in 100mL of deionized water, stirring continuously until evenly dispersed. Dissolve 10g of gelatin powder in 100mL of deionized water and heat to 60℃ until completely dissolved. Mix the fritillaria alkaloid solution and the gelatin solution, stirring continuously until evenly dispersed to obtain a mixed solution.

[0050] Step 2: Add 0.5g of polyacrylic acid to the mixed solution, and simultaneously add 5mL of 5wt% glutaraldehyde solution to the mixed solution to crosslink the hydrogel. After the crosslinking reaction is carried out for 10min, analgesic hydrogel dressing based on fritillaria alkaloids is obtained.

[0051] The tensile strength and yield strength of the analgesic hydrogel dressings based on fritillaria alkaloids obtained in Examples 1, 5-7, and Comparative Examples 1-2 were measured respectively. The test methods for tensile strength and yield strength are as described in GB / T1040.1-2025. The results are shown in the table below: Table 1. Comparison of tensile strength and yield strength of analgesic hydrogel dressings from various samples. As can be seen from the table above, the analgesic hydrogel dressings based on fritillaria alkaloids obtained by crosslinking modified polyacrylic acid in Examples 5-7 have superior tensile strength and yield strength. This indicates that the modified polyacrylic acid obtained by crosslinking polyacrylic acid with hydroxylated nano-alumina powder can significantly improve the tensile strength and yield strength of the analgesic hydrogel dressing based on fritillaria alkaloids, thereby improving the mechanical properties of the analgesic hydrogel dressing based on fritillaria alkaloids.

[0052] The number of devices and processing scale described herein are for the purpose of simplifying the description of the invention. Applications, modifications, and variations of the invention will be readily apparent to those skilled in the art.

[0053] Although embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the specification and embodiments. They can be applied to various fields suitable for the present invention. For those skilled in the art, other modifications can be easily made. Therefore, without departing from the general concept defined by the claims and their equivalents, the present invention is not limited to the specific details and illustrations shown and described herein.

Claims

1. A method for preparing an analgesic hydrogel dressing based on fritillaria alkaloids, characterized in that, Specifically, the following steps are included: Step 1: Prepare a fritillary alkaloid solution with a concentration of 0.001wt%~0.1wt% using fritillary alkaloids; Step 2: Mix the fritillaria alkaloid solution with the hydrogel matrix material precursor solution to obtain a composite solution; Add 5% to 15% (by mass) of modified polyacrylic acid to the composite solution; wherein the preparation method of the modified polyacrylic acid includes: S21. Add 20-60 nm nano-alumina powder to a 2-5 wt% H2O2 solution, stir and let stand; add 3-(2,3-epoxypropoxy)propyltrimethoxysilane to the system, stir and heat to react, cool and let stand, centrifuge to separate solid and liquid, wash the solid powder several times, and vacuum dry at 50-80℃ to obtain hydroxylated nano-alumina. S22. Hydroxylated nano-alumina is ultrasonically dispersed in a 10-20 wt% polyacrylic acid solution using polyethylene glycol-200 as a dispersant. After standing for 12-24 hours, the water is evaporated to obtain modified polyacrylic acid. Step 3: Add a cross-linking agent to the composite solution to form a three-dimensional network hydrogel structure by chemical cross-linking or physical cross-linking, thereby obtaining analgesic hydrogel dressing based on fritillaria alkaloids.

2. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In step one, the fritillaria alkaloids include one or more of the following: isosteroidal alkaloids, steroidal alkaloids, fritillaria oxalis, fritillaria oxalis, and fritillaria oxalis; wherein, the isosteroidal alkaloids include fritillaria oxalis, fritillaria oxalis A, fritillaria oxalis B, fritillaria oxalis, fritillaria oxalis, fritillaria oxalis, and fritillaria oxalis ketone.

3. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In step two, the concentration of the hydrogel matrix material in the hydrogel matrix material precursor solution is 0.5wt~50wt.

4. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In step two, the hydrogel matrix material in the precursor solution is one or more of chitosan, gelatin, chitin, cellulose, glucomannan, sodium alginate, polyacrylic acid, polyacrylamide, or hyaluronic acid.

5. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In step two, the fritillaria alkaloids in the hydrogel matrix material precursor solution are added in the form of solution, alkaloid powder, or alkaloid dispersion. The amount of Fritillaria alkaloid added to the precursor solution of the hydrogel matrix material is 0.01% to 5% of the mass of the hydrogel matrix material.

6. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In S21, the ratio of nano-alumina powder to H2O2 solution is 1~5:10~20mL:1~10mL.

7. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In S22, the ratio of polyacrylic acid solution, hydroxylated nano-alumina, and polyethylene glycol-200 is 100~250mL:1~5g:0.1~1g.

8. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In step three, the crosslinking agent is added in the form of a crosslinking agent solution with a concentration of 0-10 wt% and a volume of 0-10% of the fritillaria alkaloid solution. After the crosslinking agent is added, the crosslinking reaction includes one of the following: freeze-thaw cycle or ultraviolet irradiation.

9. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, In step three, the physical crosslinking method includes inducing crystallization or enhancing hydrogen bond interactions; the chemical crosslinking method includes adding a crosslinking agent, which includes one or more of glutaraldehyde, genipin, and epichlorohydrin.

10. The method for preparing the analgesic hydrogel dressing based on fritillaria alkaloids as described in claim 1, characterized in that, The analgesic hydrogel dressing has a water content of 50% to 99.5%.

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