Weight-losing composition containing ginger extract as well as preparation method and application of weight-losing composition

Abstract

The invention provides a weight-losing composition containing a ginger extract as well as a preparation method and application of the weight-losing composition, and relates to the technical field of health-care products. The weight-losing composition comprises the following raw materials: a fresh ginger extract, a cauliflower extract, an acanthopanax senticosus extract, an elderberry extract, krill oil, phaseolus calcaratus powder and vitamins. Through interaction of the specific raw materials including the ginger extract, the cauliflower extract, the elderberry extract and the krill oil, the weight-losing and lipid-lowering effects of the composition are remarkably improved, metabolism of a body is improved by adding the specific multivitamins, appetite is inhibited through the phaseolus calcaratus powder, satiety is improved, and the weight-losing and lipid-lowering effects of the composition are improved. The radix acanthopanacis senticosi extract is used for improving the immunity of the organism and improving the exercise tolerance, and is matched with other components, so that the weight-losing and lipid-lowering effects are further improved, and the stability is better.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of health care products, in particular to a weight loss composition containing ginger extract and a preparation method and application thereof. BACKGROUND

[0002] Obesity refers to a certain degree of obvious overweight and excessive fat layer, which is a state caused by excessive accumulation of body fat, especially triglyceride. It is not simply body weight gain, but a state of excessive accumulation of body fat tissue. Due to excessive food intake or changes in body metabolism, excessive accumulation of body fat leads to excessive weight gain and causes pathological and physiological changes or latency in human body.

[0003] The harm of obesity to human body is as follows: ①Obesity has many complications, including cerebral embolism and heart failure, higher incidence of coronary heart disease, hypertension, diabetes, cholelithiasis, and more seriously, the life span of obese people will be significantly shortened. ②People with obesity often have heat intolerance, excessive sweating, fatigue, lower limb edema, varicose veins, and dermatitis at skin folds. People with severe obesity have difficulty in moving and walking, and even heart palpitations and shortness of breath after slight activity, which affects normal life and even leads to loss of labor. ③Obesity increases the amount of oxygen consumption and the amount of work of the heart, causing myocardial hypertrophy, especially the burden on the left ventricle, which is prone to induce hypertension. Lipid deposition in the arterial wall, obesity causes stenosis and hardening of the lumen, which is prone to coronary heart disease, angina pectoris, stroke and sudden death. ④Metabolic and endocrine abnormalities caused by obesity often cause various metabolic diseases. ⑤Obese people need more oxygen due to weight gain, but the lungs cannot increase their function, and the accumulation of abdominal fat in obese people also limits the respiratory movement of the lungs, which can cause hypoxia and dyspnea, and finally lead to heart and lung failure. ⑥Compared with normal people, the cholesterol content in the bile acid of obese people increases, which exceeds the solubility in bile, so obese people are prone to high proportion of cholesterol stones. ⑦Obese people increase the risk of anesthesia, and the chances of wound dehiscence, infection and complicated pneumonia after surgery are more than those of non-obese people. ⑧The increase in body weight can cause many joints (such as spine, shoulder, elbow, hip and foot joint) to wear or tear, causing pain. ⑨Obese people can be complicated with many hernias, among which the most common is esophageal hiatal hernia in which the upper part of the stomach is displaced into the chest cavity. Therefore, weight loss is very important for the health of obese people.

[0004] At present, existing anti-obesity drugs have poor effects due to tolerance and obvious chronic toxic side effects, while many traditional herbal extracts are expected to develop into safe and effective obesity alternative therapies. Ginger is one of the most common spices, which contains many phenolic compounds that show beneficial properties for human health, including enhancing digestive function, reducing inflammation, vomiting, cancer and metabolic syndrome.

[0005] "Study on the weight loss and lipid-lowering effect of ginger oil on obese rats" (Food Science, Yang Zhigang, Zhang Yanping et al., 2007-10-30, Vol. 28 No. 9) discloses that ginger oil can effectively reduce the weight of obese rats and the weight of perirenal fat and perirenal fat, reduce the content of serum total cholesterol (TC) and serum triglyceride (TG), and reduce weight and lower lipid.

[0006] Chinese patent CN111034833A discloses a weight loss and lipid-lowering compound preparation, which contains 3-10 parts of Daidaihua, 3-10 parts of lotus leaf, 3-10 parts of fresh ginger, 1-5 parts of traditional Chinese medicine component, and 1-5 parts of lemon. Chinese patent CN114391653A discloses a sports nutrition tablet for weight loss, which comprises the following components: guarana extract, rose bata powder, green coffee powder, fresh ginger powder, lotus leaf powder, mulberry leaf extract, raspberry powder, orange peel powder, black pepper powder, ginseng powder, epigallocatechin gallate, white kidney bean powder, vitamin B1, vitamin B2, sugar alcohol, microcrystalline cellulose, magnesium stearate and coating powder. Chinese patent CN103053764A discloses a health tea, which is made of 3-10 parts of soluble pearl powder, 5-15 parts of lotus leaf extract, 1-5 parts of fresh ginger extract, 20-40 parts of Chinese date extract, 5-10 parts of dandelion extract, 10-15 parts of konjac extract, 1-5 parts of L-carnitine, 20-30 parts of maltodextrin, 0.5-2 parts of fat-soluble vitamin C. The products involved in the above patents all contain fresh ginger.

[0007] In summary, it is the research direction of researchers in the field to develop a health care product mainly containing fresh ginger, optimize the preparation method of fresh ginger, and compound with other components to achieve better weight loss and lipid-lowering effect, and better meet the needs of obese people. SUMMARY

[0008] The present application provides a weight loss composition containing ginger extract and its preparation method and application, which has better weight loss and lipid-lowering effect under the specific components, ratio and preparation method.

[0009] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows: On the one hand, the present application provides a weight loss composition containing ginger extract, which comprises the following raw materials: ginger extract, broccoli extract, acanthopanax extract, sambucus nigra extract, phosphorus shrimp oil, red bean powder and vitamin.

[0010] Preferably, the vitamin is vitamin C, vitamin B6 and vitamin D.

[0011] Preferably, the mass ratio of the vitamin C, vitamin B6 and vitamin D is (5-8):(1-3):(2-4).

[0012] Further preferably, the mass ratio of the vitamin C, vitamin B6 and vitamin D is 6:2:3.

[0013] Preferably, the mass ratio of the ginger extract, broccoli extract, krill oil and elderberry extract is (20-30):(10-20):(10-15):(5-10).

[0014] Further preferably, the mass ratio of the ginger extract, broccoli extract, krill oil and elderberry extract is 25:15:12:8.

[0015] Preferably, the preparation method of the ginger extract comprises the following steps: mixing ginger with an ethanol solution, gradient alcohol extraction, combining the extraction liquid, concentrating, and drying.

[0016] Further preferably, the preparation method of the ginger extract comprises the following steps: grinding ginger to 40-60 mesh, adding 30wt%-40wt% of an ethanol aqueous solution, the mass of the ginger to the volume of the ethanol aqueous solution being 1g:8-15mL; first extracting at 35-45℃ for 1-1.5h to obtain extraction liquid 1 and residue 1; adding 45wt%-55wt% of an ethanol aqueous solution to the residue 1, the mass of the ginger to the volume of the ethanol aqueous solution being 1g:8-15mL; second extracting at 45-55℃ for 1-1.5h to obtain extraction liquid 2 and residue 2; adding 70wt%-80wt% of an ethanol aqueous solution to the residue 2, the mass of the ginger to the volume of the ethanol aqueous solution being 1g:8-15mL; third extracting at 55-65℃ for 1-1.5h to obtain extraction liquid 3, combining the extraction liquid 1, extraction liquid 2 and extraction liquid 3, concentrating, and drying.

[0017] Preferably, the preparation method of the broccoli extract comprises the following steps: grinding broccoli and enzymatic extraction, concentrating the extraction liquid, and drying.

[0018] Further preferably, the preparation method of the broccoli extract comprises the following steps: grinding broccoli, mixing with water, adding a compound enzyme, adjusting the pH to 5-6, extracting at 40-50℃ for 1-2h, concentrating the extraction liquid, and drying.

[0019] Preferably, the compound enzyme is cellulase and bromelain with an enzyme activity ratio of 40-60U:10-20U.

[0020] Further preferably, the compound enzyme is cellulase and bromelain with an enzyme activity ratio of 50U:15U.

[0021] Preferably, the mass ratio of the enzymolysis activity of the complex enzyme to the broccoli is 100-150 U / g.

[0022] Further preferably, the mass ratio of the enzymolysis activity of the complex enzyme to the broccoli is 130 U / g.

[0023] Preferably, the following raw materials are included according to mass fraction: ginger extract 20-30 parts, broccoli extract 10-20 parts, acanthopanax extract 3-8 parts, elderberry extract 5-10 parts, krill oil 10-15 parts, red bean powder 10-18 parts, and vitamin 1-4 parts.

[0024] Further preferably, the following raw materials are included according to mass fraction: ginger extract 23-28 parts, broccoli extract 13-18 parts, acanthopanax extract 4-6 parts, elderberry extract 6-9 parts, krill oil 11-14 parts, red bean powder 12-16 parts, and vitamin 2-3 parts.

[0025] More preferably, the following raw materials are included according to mass fraction: ginger extract 25 parts, broccoli extract 15 parts, acanthopanax extract 5 parts, elderberry extract 8 parts, krill oil 12 parts, red bean powder 15 parts, and vitamin 3 parts.

[0026] In a second aspect, the present application provides a preparation method of the above-mentioned weight loss composition, including the following step: mixing the raw materials.

[0027] In a third aspect, the present application provides an application of the above-mentioned weight loss composition in preparing health products with weight loss and lipid-lowering effects.

[0028] Preferably, the health products further include other auxiliary materials acceptable in the health product field.

[0029] Preferably, the health products can be prepared into solid beverage preparations, liquid beverage preparations, nutrition bars, powders, etc.

[0030] Compared with the prior art, the present application has the following beneficial effects: 1、The application significantly improves the weight loss and lipid-lowering effect through specific raw material interaction: ginger extract can activate the AMPK pathway, promote fat oxidation, effectively inhibit weight gain and hyperlipidemia caused by lipid metabolism disorder, and may increase energy consumption through thermogenic effect; on the other hand, it can improve abnormal glucose tolerance and improve insulin sensitivity. Cauliflower extract can significantly inhibit the activity of pancreatic amylase in the intestinal tract, hinder or delay the hydrolysis and digestion of the main carbohydrates in food, and reduce the decomposition and absorption of starch and sugar substances in food. Phosphorus shrimp oil is rich in Omega-3 fatty acids, which not only has anti-inflammatory effect, but also can significantly improve metabolism. Sambucus extract can resist oxidation, dissolve fat, and remove turbidity and oil. The above components interact with each other to significantly improve the weight loss and lipid-lowering effect of the product.

[0031] 2、The composition of the application improves the metabolism of the body by adding specific complex vitamins, suppresses appetite and improves satiety by adding red bean powder, improves immunity and exercise endurance of the body by adding acanthopanax extract, and significantly improves the effect of weight loss and lipid-lowering in cooperation with other components.

[0032] 3、The specific components of the application are combined with specific amounts and preparation methods, which further strengthen the effect and have better stability. DETAILED DESCRIPTION

[0033] In order to make the technical means, creative features, purposes and effects realized by the application easy to understand, the following specific embodiments are further illustrated, but the following embodiments are only preferred embodiments of the application, not all. Based on the embodiments in the embodiments, other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the application. It is worth noting that the raw materials used in the application are ordinary commercially available products, and their sources are not specifically limited. The technology and scientific terms used in the embodiments have the meanings generally understood by those skilled in the art to which the application belongs.

[0034] Acanthopanax extract was purchased from Xi'an Feida Biotechnology Co., Ltd., with the product number FDBL-162.

[0035] Sambucus extract was purchased from Xi'an Dingchu Biotechnology Co., Ltd., with the product number DC-dsg.

[0036] Example 1 A weight loss composition containing ginger extract (1) According to the mass fraction, the following raw materials are included: ginger extract 25 parts, cauliflower extract 15 parts, acanthopanax extract 5 parts, sambucus extract 8 parts, phosphorus shrimp oil 12 parts, red bean powder 15 parts, vitamin C 1.63 parts, vitamin B6 0.55 parts and vitamin D 0.82 parts.

[0037] The preparation method of the ginger extract comprises the following steps: the ginger is cleaned, dried, and then crushed to 50 mesh, 35wt% ethanol aqueous solution is added, the mass of the ginger is 1g and the volume of the ethanol aqueous solution is 10mL; the ginger is extracted for the first time at 40℃ for 1.5h to obtain an extract 1; the residue is added with 50wt% ethanol aqueous solution, the mass of the ginger is 1g and the volume of the ethanol aqueous solution is 12mL; the ginger is extracted for the second time at 50℃ for 1h to obtain an extract 2; the residue is added with 75wt% ethanol aqueous solution, the mass of the ginger is 1g and the volume of the ethanol aqueous solution is 15mL; the ginger is extracted for the third time at 60℃ for 1h to obtain an extract 3; the extracts are combined, concentrated, and dried.

[0038] The preparation method of the cauliflower extract comprises the following steps: the cauliflower is cleaned, dried, and then ground, mixed with water, and then added with a compound enzyme (the enzyme activity ratio of cellulase and bromelain is 50U:15U), the enzyme hydrolysis activity of the compound enzyme and the mass of the cauliflower are 130U / g, the pH is adjusted to 5.5, and the cauliflower is extracted at 45℃ for 1.5h; the extract is concentrated and dried.

[0039] (2) A preparation method of the weight-reducing composition containing the ginger extract: the raw materials are uniformly mixed.

[0040] Example 2 A weight-reducing composition containing a ginger extract (1) The weight-reducing composition contains the following raw materials in parts by mass: 20 parts of the ginger extract, 20 parts of the cauliflower extract, 3 parts of the acanthopanax senticosus extract, 10 parts of the elderberry extract, 10 parts of the krill oil, 18 parts of the red bean powder, 0.625 parts of vitamin C, 0.125 parts of vitamin B6, and 0.25 parts of vitamin D.

[0041] The preparation method of the ginger extract comprises the following steps: the ginger is cleaned, dried, and then crushed to 40 mesh, 30wt% ethanol aqueous solution is added, the mass of the ginger is 1g and the volume of the ethanol aqueous solution is 8mL; the ginger is extracted for the first time at 35℃ for 1.5h to obtain an extract 1; the residue is added with 55wt% ethanol aqueous solution, the mass of the ginger is 1g and the volume of the ethanol aqueous solution is 15mL; the ginger is extracted for the second time at 55℃ for 1.5h to obtain an extract 2; the residue is added with 70wt% ethanol aqueous solution, the mass of the ginger is 1g and the volume of the ethanol aqueous solution is 8mL; the ginger is extracted for the third time at 55℃ for 1.5h to obtain an extract 3; the extracts are combined, concentrated, and dried.

[0042] The preparation method of the broccoli extract comprises the following steps: after the broccoli is washed, dried and ground, the broccoli is mixed with water and then a compound enzyme (the enzyme activity ratio of cellulase and bromelain is 40U:10U) is added, the enzyme hydrolysis activity of the compound enzyme and the mass of the broccoli are in a ratio of 100U / g, the pH is adjusted to 5.0, the extraction is carried out at 40℃ for 2h, the extract is concentrated and dried.

[0043] (2) The preparation method of the weight-reducing composition containing the ginger extract: uniformly mixing raw materials.

[0044] Example 3 A weight-reducing composition containing a ginger extract (1) The weight-reducing composition contains the following raw materials in parts by mass: 30 parts of the ginger extract, 10 parts of the broccoli extract, 8 parts of the acanthopanax extract, 5 parts of the elderberry extract, 15 parts of the krill oil, 10 parts of the red bean powder, 2.1 parts of vitamin C, 0.8 parts of vitamin B6 and 1.1 parts of vitamin D.

[0045] The preparation method of the ginger extract comprises the following steps: after the ginger is washed, dried and ground to 60 meshes, 40wt% of the ethanol aqueous solution is added, the mass of the ginger and the volume of the ethanol aqueous solution are in a ratio of 1g:15mL; the first extraction is carried out at 45℃ for 1.0h to obtain extract 1; the residue is added with 45wt% of the ethanol aqueous solution, the mass of the ginger and the volume of the ethanol aqueous solution are in a ratio of 1g:10mL; the second extraction is carried out at 45℃ for 1.5h to obtain extract 2; the residue is added with 80wt% of the ethanol aqueous solution, the mass of the ginger and the volume of the ethanol aqueous solution are in a ratio of 1g:15mL; the third extraction is carried out at 65℃ for 1.0h to obtain extract 3; the extracts are combined, concentrated and dried.

[0046] The preparation method of the broccoli extract comprises the following steps: after the broccoli is washed, dried and ground, the broccoli is mixed with water and then a compound enzyme (the enzyme activity ratio of cellulase and bromelain is 60U:20U) is added, the enzyme hydrolysis activity of the compound enzyme and the mass of the broccoli are in a ratio of 150U / g, the pH is adjusted to 5.5, the extraction is carried out at 50℃ for 1h, the extract is concentrated and dried.

[0047] (2) The preparation method of the weight-reducing composition containing the ginger extract: uniformly mixing raw materials.

[0048] Comparative Example 1 A weight-reducing composition containing a ginger extract, compared with Example 1, only the amount of the raw materials is changed, specifically: (1) The weight-reducing composition contains the following raw materials in parts by mass: 35 parts of the ginger extract, 8 parts of the broccoli extract, 2 parts of the acanthopanax extract, 3 parts of the elderberry extract, 8 parts of the krill oil, 20 parts of the red bean powder, 3.82 parts of vitamin C, 1.27 parts of vitamin B6 and 1.91 parts of vitamin D.

[0049] The rest is consistent with example 1.

[0050] Comparative example 2 A weight loss composition containing ginger extract, compared with example 1, replacing cauliflower extract with white kidney bean powder, replacing krill oil with raspberry powder, and replacing elderberry extract with epigallocatechin gallate. Specifically: (1) According to the mass fraction, it includes the following raw materials: ginger extract 25 parts, white kidney bean powder 15 parts, acanthopanax extract 5 parts, epigallocatechin gallate 8 parts, raspberry powder 12 parts, red bean powder 15 parts, vitamin C 1.63 parts, vitamin B6 0.55 parts and vitamin D 0.82 parts.

[0051] Among them, the preparation method of ginger extract includes the following steps: after the ginger is washed, dried and crushed to 50 mesh, 35wt% ethanol aqueous solution is added, the mass of ginger and the volume of ethanol aqueous solution are 1g:10mL; first extraction at 40℃ for 1.5h, get extract 1; add 50wt% ethanol aqueous solution to the residue, the mass of ginger and the volume of ethanol aqueous solution are 1g:12mL; second extraction at 50℃ for 1.0h, get extract 2; add 75wt% ethanol aqueous solution to the residue, the mass of ginger and the volume of ethanol aqueous solution are 1g:15mL; third extraction at 60℃ for 1h, get extract 3, combine the extracts, concentrate and dry.

[0052] (2) The preparation method of the weight loss composition containing ginger extract: mix the raw materials uniformly.

[0053] Comparative example 3 A weight loss composition, compared with example 1, replacing ginger extract with barley extract.

[0054] (1) According to the mass fraction, it includes the following raw materials: barley extract 25 parts, cauliflower extract 15 parts, acanthopanax extract 5 parts, elderberry extract 8 parts, krill oil 12 parts, red bean powder 15 parts, vitamin C 1.63 parts, vitamin B6 0.55 parts and vitamin D 0.82 parts.

[0055] Among them, the preparation method of cauliflower extract includes the following steps: after the cauliflower is washed, dried and ground, it is mixed with water and then compound enzyme (the enzyme activity ratio of cellulase and bromelain is 50U:15U) is added, the enzyme hydrolysis activity of compound enzyme and the mass of cauliflower are 130U / g, the pH is adjusted to 5.5, and the extraction is carried out at 45℃ for 1.5h, the extract is concentrated and dried.

[0056] (2) The preparation method of the above weight loss composition: mix the raw materials uniformly.

[0057] Test Example 1 Stability Test The stability of the compositions prepared in Example 1-Example 3 and Comparative Example 1-Comparative Example 2 was determined by HPLC method, and the gingerols in each composition were tested at 0, 3, 6 months, respectively, and then the loss rate of gingerols in the compositions after 3 months, 6 months was recorded (compared with the content on the day of preparation).

[0058] Chromatographic conditions: Chromatographic column: C18 reversed-phase chromatographic column (such as Agilent Grace Smart RP-C18).

[0059] Mobile phase: acetonitrile-0.1% formic acid aqueous solution (40:60) or acetonitrile-0.1% acetic acid aqueous solution.

[0060] Flow rate: 1.0 mL / min, detection wavelength: 280 nm.

[0061] Table 1. Loss rate of gingerol / %

[0062] From Table 1, it can be seen that the stability of the weight loss composition prepared by the specific amount of the specific component of the present application is significantly better than that of the weight loss composition prepared by Comparative Example 1-Comparative Example 2.

[0063] Test Example 2 Animal Experiment: Improving Effect of Composition on Obese Rats 1. Materials and Methods 1.1 Materials 1.1.1 Instruments: FA1104N electronic balance (Shanghai Minqiao Precision Scientific Instrument Co., Ltd.), animal balance, TGL-16aR ultracentrifuge (Shanghai Anting Scientific Instrument Factory), EPOCH enzyme label instrument (BioTek, USA), etc.

[0064] 1.1.2 Reagents: High-fat feed (item number: D12451) was purchased from Beijing Huafukang Biotechnology Co., Ltd.; Common mouse maintenance feed was purchased from Changchun Yis Experimental Animal Technology Co., Ltd.; Hematoxylin-eosin (Hematoxylin-eosin, HE) staining kit was purchased from Beijing Solaybao Technology Co., Ltd.; Rat triglyceride (TG), total cholesterol (TC) detection kit were purchased from Jiangsu Fia Biological Technology Co., Ltd.; Rat superoxide dismutase (SOD) was purchased from Nanjing Jiancheng Biological Engineering Institute, and alanine aminotransferase (ALT) detection kit was purchased from Jiangsu Aidsen Biological Technology Co., Ltd.

[0065] 1.1.3 Experimental animals SPF level male SD rats 72, body weight 180-220 g, purchased from Beijing Huafukang Biotechnology Co., Ltd. [License No. SCXK (Jing) 2024-0003]. Natural light cycle lighting (12 h light / dark alternation), feeding temperature 22-24℃, relative humidity 55%-60%.

[0066] 1.2 Model construction and animal grouping After one week of adaptive feeding, the rats were randomly divided into 9 groups (n = 8) according to body weight. The recommended amount of ginger powder and the body surface area standardization method for humans and rats were combined to convert the dosages, and the dosage of orlistat (positive control group) was converted. The groups are as follows: Model control group, normal control group, positive control group (orlistat, 31.71 mg / kg), example group (example 1 group, example 2 group, example 3 group, 12.68 mg / kg), and comparison group (comparison 1 group, comparison 2 group, comparison 3 group, 12.68 mg / kg).

[0067] In addition to the normal control group being given ordinary maintenance feed, the remaining groups of rats were given high-fat feed for 6 weeks to establish an obese rat model. In addition to the model control group and the normal control group being given 12.68 mg / kg of distilled water by gavage, the positive drug group was given orlistat by gavage, and the example group and the comparison group were given the respective prepared compositions by gavage.

[0068] 1.3 Detection method 1.3.1 Observation of rat state During feeding, the neck circumference, waist circumference, body weight, body length, and food intake were recorded every week. After the last administration, the rats were fasted for 12 h without water, and the rat food intake, body weight, and Lee's index were measured and calculated.

[0069] 1.3.2 Detection of rat physiological indicators (fat and organs) After the experiment, the rats were killed by cervical dislocation, and the rat liver, kidney, and other organs were carefully separated, rinsed clean with 0.9% physiological saline, and excess water was absorbed with filter paper. The mass was weighed and recorded using an electronic balance, and then stored at -80℃ for subsequent indicator detection.

[0070] 1.3.3 Detection of rat serum indicators After the last administration, the rats were fasted for 12 h without water, and after anesthesia, the blood was taken from the abdominal aorta, and the serum was separated by centrifugation at 4000 r / min for 15 min at room temperature and stored at -20℃ for detection. The levels of TC, TG, and other indicators in the serum were determined according to the kit steps and determination requirements.

[0071] 1.3.4 Detection of rat liver indicators About 0.1 g of liver tissue sample was weighed, ground and homogenized, and the supernatant was taken for detection. Alanine aminotransferase (ALT) and superoxide dismutase (SOD) were detected.

[0072] 1.4 Data statistics and processing: The experimental data were recorded by double-blind method, and the experimental results were analyzed by GraphPad Prism software (Version 10.1.2, USA). The data were expressed as mean ± standard error of mean. The experimental data between different groups were compared by One-Way Analysis of Variance (ANOVA) and Tukey test. p<0.05 indicated that the difference between groups was statistically significant.

[0073] 1.5 Results 1.5.1 Rat state During the experiment, there was no significant difference in food intake between rats in each group (p>0.05), indicating that the compositions in each group did not affect the rats' eating and had no effect on their appetite. The rats in each group were observed, and compared with the normal control group, the body weight of the rats in the model control group was significantly increased at each week. After intervention with the compositions prepared in the examples and the comparative examples, the body weight of the rats in each drug group was reduced to different extents compared with the model control group. The body weight of the rats at the sixth week of modeling is shown in Table 2. Compared with the normal control group, the body weight of the rats in the model control group was significantly increased, indicating that the modeling was successful. After administration, the body weight of the rats in the example group was significantly reduced compared with the model control group (p<0.01).

[0074] By comparing the rats in each group, as shown in Table 2, the Lee's index of the rats in the model control group was significantly increased compared with the normal control group (p<0.01). After administration intervention, the Lee's index of the rats in the positive drug group was significantly reduced (p<0.01), and the Lee's index of the rats in the example group was also significantly reduced (p<0.01). It is indicated that the compositions prepared by the present application can effectively improve the basic indicators of high-fat diet-induced obese rats.

[0075] Table 2. Body weight at the sixth week of modeling

[0076] Note: compared with the normal control group, && p<0.01; compared with the model control group, # p<0.5, ## p<0.01.

[0077] 1.5.2 Effect on fat coefficient and organ coefficient of rats Fat coefficient refers to the ratio of epididymal fat mass to body weight in rats, usually expressed as a percentage. This index can preliminarily reflect the relative content of fat in rats, and provide a basis for evaluating the degree of obesity. After 6 weeks of high-fat diet, as shown in Table 3 below, the fat coefficient of the obese rat model in the model control group was significantly increased (p<0.01). After the intragastric intervention of the example group, the fat coefficient of the rats in each group decreased significantly, and the fat coefficient of the rats in the high-dose group was significantly lower than that in the model control group (p<0.05).

[0078] The organ coefficient refers to the ratio of the weight of each organ of the rat to the body weight, which can reflect the fat deposition and metabolic abnormalities of obese rats in each organ (such as liver and kidney). As shown in Table 3 below, the comparison results of the organ coefficients of the rats in each group showed that, compared with the normal control group, the liver coefficient and kidney coefficient of the rats in the model control group were significantly increased (p<0.01). After drug intervention, the kidney coefficients of the rats in the positive drug group and the example group were significantly decreased (p<0.05 or p<0.01). In addition, the liver coefficient of the rats in the example group also decreased significantly (p<0.05), and the effect was better than that of the positive drug orlistat. The above results suggest that the composition prepared in the example of the present application can play a significant role in weight loss by regulating the lipid deposition or metabolic process of the liver.

[0079] Table 3. Effect on fat coefficient and organ coefficient of rats

[0080] Note: compared with the normal control group, && represent p<0.01; compared with the model control group, # represent p<0.5, ## represent p<0.01.

[0081] 1.5.3 Effect on lipid levels in serum and liver of rats After 6 weeks of experimental intervention, the serum lipid levels of the rats in each group were shown in Table 4 below. Compared with the normal control group, the TC and TG levels in the serum of the rats in the model control group were significantly increased (p<0.01). The administration intervention of the example group could effectively reduce the TC and TG in the serum (p<0.01).

[0082] and the example group intervention reduced the serum TC level better than the positive drug orlistat, indicating that the weight loss composition prepared in the example of the present application can play a role in slowing down obesity by inhibiting the lipid levels and lipid deposition in the serum and liver. And compared with the positive drug orlistat, the example group intervention showed better effect in reducing the serum TC level and TG content. The above all suggest that the example group may inhibit the synthesis and accumulation of lipids in the serum and liver, thereby effectively alleviating the process of obesity.

[0083] Table 4. Effects on serum lipid levels of rats

[0084] Note: compared with the normal control group, && represent p < 0.01; compared with the model control group, # represent p < 0.5, ## represent p < 0.01.

[0085] 1.5.4 Effects on the liver oxidative stress level of rats As shown in Table 5, compared with the normal control group, the SOD and ALT levels in the liver of the model control group of rats were significantly increased (p < 0.01). It showed that the liver antioxidant capacity of obese rats was decreased, and the degree of lipid peroxidation was increased. Compared with the model control group, the ALT level in the liver of the positive drug group of rats was significantly decreased (p < 0.01), and the SOD level was significantly increased (p < 0.01); the ALT of the liver of the example group of rats was significantly decreased (p < 0.01), and the SOD level was significantly increased (p < 0.01). It suggested that the composition prepared by the example group of the application could effectively improve the liver antioxidant level of rats and improve the oxidative stress of the liver of obese rats.

[0086] Table 5. Effects on the liver oxidative stress level of rats

[0087] Note: compared with the normal control group, && represent p < 0.01; compared with the model control group, # represent p < 0.5, ## represent p < 0.01.

[0088] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by those skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A weight-loss composition containing ginger extract, characterized in that, It includes the following ingredients: ginger extract, cauliflower extract, Acanthopanax senticosus extract, elderberry extract, krill oil, red bean powder, and vitamins.

2. The weight-loss composition according to claim 1, characterized in that, The vitamins are a mixture of vitamin C, vitamin B6 and vitamin D in a mass ratio of (5-8):(1-3):(2-4).

3. The weight-loss composition according to claim 1, characterized in that, The mass ratio of the ginger extract, cauliflower extract, krill oil and elderberry extract is (20-30):(10-20):(10-15):(5-10).

4. The weight-loss composition according to claim 1, characterized in that, The preparation method of the ginger extract includes the following steps: mixing ginger with ethanol solution, performing gradient ethanol extraction, combining the extracts, concentrating, and drying.

5. The weight-loss composition according to claim 4, characterized in that, The preparation method of the ginger extract includes the following steps: pulverizing ginger to 40-60 mesh, adding 30wt%-40wt% ethanol aqueous solution, with a ginger mass to ethanol aqueous solution volume ratio of 1g:8-15mL; first extraction at 35-45℃ for 1-1.5h to obtain extract 1 and residue 1; adding 45wt%-55wt% ethanol aqueous solution to residue 1, with a ginger mass to ethanol aqueous solution volume ratio of 1g:8-15mL; second extraction at 45-55℃ for 1-1.5h to obtain extract 2 and residue 2; adding 70wt%-80wt% ethanol aqueous solution to residue 2, with a ginger mass to ethanol aqueous solution volume ratio of 1g:8-15mL; third extraction at 55-65℃ for 1-1.5h to obtain extract 3; combining extract 1, extract 2 and extract 3, concentrating and drying.

6. The weight-loss composition according to claim 1, characterized in that, The preparation method of the cauliflower extract includes the following steps: grinding cauliflower and then enzymatically extracting it, concentrating the extract, and drying it.

7. The weight-loss composition according to claim 6, characterized in that, The preparation method of the cauliflower extract includes the following steps: grinding cauliflower, mixing with water, adding a compound enzyme, adjusting the pH to 5-6, extracting at 40-50℃ for 1-2 hours, concentrating the extract, and drying; the compound enzyme is cellulase and bromelain with an enzyme activity ratio of 40-60U:10-20U; the enzymatic hydrolysis activity of the compound enzyme and the mass ratio of cauliflower are 100-150U / g.

8. The weight-loss composition according to claim 1, characterized in that, By weight, it includes the following ingredients: 20-30 parts ginger extract, 10-20 parts cauliflower extract, 3-8 parts Acanthopanax senticosus extract, 5-10 parts elderberry extract, 10-15 parts krill oil, 10-18 parts red bean powder, and 1-4 parts vitamins.

9. A method for preparing the weight-loss composition according to any one of claims 1-8, characterized in that, The process includes the following steps: mixing the raw materials.

10. The use of the weight-loss composition according to any one of claims 1-8 in the preparation of a health product having weight-loss and lipid-lowering effects.

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