Method for producing polyunsaturated fatty acid

By adding rapeseed protein powder to the fermentation medium to activate Schizochytrium seeds, the problem of low EPA and DHA production by Schizochytrium was solved, resulting in a significant increase in EPA and DHA production to meet industrial needs.

CN121160809APending Publication Date: 2025-12-19OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202511144149.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-15
Publication Date
2025-12-19

AI Technical Summary

Technical Problem

In existing technologies, the content and yield of polyunsaturated fatty acids EPA and DHA synthesized by Schizochytrium are low, making it difficult to meet the needs of industrial production.

Method used

By adding rapeseed protein powder as a nitrogen source to the fermentation medium, combined with appropriate culture temperature and rotation speed, the seeds of Schizochytrium were activated, promoting their high metabolic activity. The polyphenols in the rapeseed protein powder were used to promote the synthesis of EPA and DHA.

Benefits of technology

It significantly increased the production and proportion of EPA and DHA, meeting the needs of industrial production.

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Abstract

The invention provides a method for producing polyunsaturated fatty acid, and belongs to the technical field of biological fermentation. The method for producing polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid from schizochytrium limacinum comprises the following steps: activating schizochytrium limacinum in a seed culture medium to obtain a schizochytrium limacinum seed culture solution; inoculating the schizochytrium limacinum seed culture solution into a fermentation culture medium for culturing, wherein the fermentation culture medium comprises rapeseed protein powder; and extracting the culture solution in the cultured fermentation culture medium to obtain the polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid. According to the present invention, the yield of polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid can be significantly improved.
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