Bionic simulation collection device and analysis method for dynamic migration volume of taste substances in taste tipping paper

Abstract

The invention belongs to the technical field of cigarette tipping paper taste substance analysis, and particularly relates to a bionic simulation collection device and an analysis method for the dynamic migration quantity of taste substances in taste tipping paper. The bionic simulation collection device comprises a simulation lip, a simulation oral cavity, a smoke collector and a smoke suction machine which are sequentially communicated from upstream to downstream. The analysis method comprises the following steps: S1, connecting an artificial lip and a simulated oral cavity with a smoking machine, and infiltrating with artificial saliva; s2, the taste tipping paper of the cigarette is fixed to an artificial lip, the surface of the artificial lip is attached to the surface of a filter tip, and in the smoking interval process, the cigarette is inserted and pulled out; s3, after smoking is finished, the artificial lip cleaning fluid and the simulated oral cavity cleaning fluid are collected, and the migration amount of the taste substances is detected. The method is closer to the actual cigarette smoking process, and powerful technical support can be provided for cigarette product research and development, smoke physiological feeling, smoking, health and other basic research.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of taste substance analysis of cigarette tipping paper, and particularly relates to a bionic simulation collection device and an analysis method for dynamic migration amount of taste substances in taste tipping paper. BACKGROUND

[0002] The consumer experience is the ultimate research and development goal of a cigarette product. Due to the diversified pursuit and individualized requirements of consumers for smoke, the cigarette product pays more and more attention to the online sensory experience of smoke. The cigarette tipping paper, also known as the water-based paper, is an important packaging material of the smoking part of the cigarette product, and is mainly used for connecting the filter and the main body of the cigarette. It is also the only part of the cigarette product that contacts the oral cavity (mainly the lips) of the human body. The addition of different taste substances in the cigarette tipping paper can not only effectively improve the direct taste experience of consumers during the smoking process, but also to a certain extent, improve the quality of the cigarette, highlight the taste characteristics and brand influence. Therefore, using different taste tipping paper to increase the flavor of smoke, to improve the richness of smoke, and to realize diversification have become the focus and key of the research and development of cigarette enterprises.

[0003] During the smoking process, the consumer will insert and pull out the cigarette filter in the mouth multiple times with the inhalation and exhalation of smoke, and the tipping paper will also be rubbed and contacted with the mouth (mainly the lips) of the consumer. In this process, the taste substances on the taste tipping paper will also be dynamically migrated due to the dynamic friction, and the infiltration and contact of the saliva of the lips, thereby causing different taste experiences of the smoking consumer. However, since this process involves continuous smoking behavior, and involves human senses and organs, the process is complex and variable. At present, there are few reports and literatures about the dynamic migration of taste substances in taste tipping paper, resulting in a blank in the evaluation of taste tipping paper and the stability evaluation of the product. Therefore, it is particularly important to provide a device and a method which can simulate the actual smoking situation of the consumer and effectively evaluate the dynamic migration of taste substances.

[0004] To solve the above problems, the present application is proposed. SUMMARY

[0005] The first aspect of the present application provides a bionic simulation collection device for dynamic migration amount of taste substances in taste tipping paper, which comprises: a simulation lip 1, a simulation oral cavity 3, a smoke collector 4 and a smoking machine 11 connected in sequence from upstream to downstream.

[0006] The simulation oral cavity 3 is also communicated with the air extraction device 8 through the third valve 10-3, so as to extract the gas in the simulation oral cavity 3 through the air extraction device 8.

[0007] The simulated mouth 1 has a smoke passage inside, which is communicated to the simulated oral cavity 3 through a connecting hose 12.

[0008] A first valve 10-1 is arranged on the smoke inlet pipe.

[0009] The simulated oral cavity 3 has a cavity inside for smoke diffusion, which is communicated to the smoke collector 4.

[0010] The downstream end of the smoke collector 4 is communicated to the smoking machine 11 through a second valve 10-2.

[0011] The smoke collector 4 is internally provided with a piece of smoke collection and adsorption material 5.

[0012] Preferably, the air extraction device 8 is a vacuum pump.

[0013] Preferably, the simulated mouth 1 is a porous hollow columnar structure soaked with artificial saliva.

[0014] The simulated mouth 1 is made of silica gel sponge.

[0015] Preferably, the inner wall of the simulated oral cavity 7 is provided with a simulated oral mucosa soaked with artificial saliva.

[0016] The simulated oral mucosa is a layer of hydrophilic adsorption material.

[0017] Preferably, the cigarette holder 2 is an elastic silica gel holder for holding cigarettes of different sizes.

[0018] Preferably, the simulated oral cavity 7 is a glass bin.

[0019] Preferably, the simulated mouth 1 has a cigarette holder 2 inside.

[0020] The second aspect of the application provides an analysis method for the dynamic migration amount of taste substances in taste tipping paper, comprising the following steps: S1, connecting the simulated mouth 1, the simulated oral cavity 3 and the smoking machine 11, and soaking the simulated mouth 1 and the simulated oral cavity 3 with artificial saliva; S2, fixing the taste tipping paper 11 of the cigarette 6 on the simulated mouth 1 through the cigarette holder 2, and making the surface of the simulated mouth 1 close to the filter surface of the cigarette 6 with a proper mouth depth, inserting and pulling out the cigarette during the suction interval; S3, collecting the cleaning liquid of the simulated mouth 1 and the cleaning liquid of the simulated oral cavity 3 after smoking, and detecting the migration amount of taste substances by using chromatography method.

[0021] Specifically, the analysis method comprises:

[0022] Step a. Place the cigarette 6 with the taste tipping paper 7 into the simulated lip 1 through the cigarette holder 2, and let the taste tipping paper 7 contact the simulated lip 1;

[0023] Step b. Soak the simulated lip 1 with artificial saliva evenly, and soak the inner wall of the simulated oral cavity 3 with artificial saliva;

[0024] Step c. Place the smoke collection and adsorption material 5 into the smoke collector 4, and connect the simulated lip 1, the simulated oral cavity 3, the smoke collector 4, and the smoking machine 11 through the connecting hose 12 and the first valve 10-1 and the second valve 10-2;

[0025] Connect the vacuum pump 8 with the simulated oral cavity 3 through the third valve 10-3;

[0026] Step d. After all the components are connected, open the first valve 10-1 and the second valve 10-2, and close the third valve 10-3, and check the air tightness;

[0027] Place the cigarette 6 into the simulated lip 1 through the cigarette holder 2, and start the smoking machine 11 to light the cigarette 6 and start smoking;

[0028] Step e. After the start of smoking, the first puff of smoke enters the simulated oral cavity 3 through the simulated lip 1 and stays for a short time, and then is adsorbed by the smoke adsorption material 5 when passing through the smoke collector 4;

[0029] Subsequently, the smoking machine enters an interval waiting time, removes the cigarette 6 from the simulated lip 1, closes the first valve 10-1 and the second valve 10-2, opens the third valve 10-3, and opens the air exhaust device 8 to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of a smoker spitting smoke;

[0030] Step f. 5 seconds before the start of the second puff of the smoking machine, first open the first valve 10-1 to quickly restore the negative pressure in the simulated oral cavity 3, close the third valve 10-3, open the second valve 10-2, and timely insert the cigarette 6, keep the depth of the cigarette 6 in the mouth consistent with before, and let the taste tipping paper 7 contact the simulated lip 1;

[0031] Step g. Repeat steps e and f multiple times until the end of smoking;

[0032] Step h. After the end of smoking, remove the simulated lip 1 and rinse it with artificial saliva, collect the rinsing liquid for component analysis of the taste substances;

[0033] Collect the artificial saliva in the simulated oral cavity 3, and rinse the inner wall of the simulated oral cavity 3 multiple times with artificial saliva, combine the rinsing liquid and the originally collected artificial saliva in the simulated oral cavity 3, and perform evaluation analysis of the migration amount of the taste substances.

[0034] Preferably, a layer of hydrophilic adsorption material is attached to the inner wall of the simulated oral cavity 3 as a simulated oral mucosa to adsorb artificial saliva.

[0035] Preferably, when it is necessary to analyze the dynamic migration amount of flavor substances in the flavor wrapper under the condition of long-term oral smoking, the repeated steps e.f are modified as follows:

[0036] The state of holding the cigarette filter is maintained, and the contact state of the flavor wrapper 7 and the simulated lips 1 is maintained; the first valve 10-1 is closed, the smoking machine 11 is kept in the state of suction, but no cigarette smoke is sucked; thus, one or several puffs are sucked through the set suction program of the smoking machine 11.

[0037] The analysis of the dynamic migration amount of flavor substances in the flavor wrapper and the related simulation device of the present application can improve the conventional static extraction method into a dynamic migration process. The development and application of the present application can provide strong technical support and development basis for the basic researches of cigarette product production, product research and development, smoking taste response, smoking and health, etc.

[0038] Specifically, the dynamic migration amount analysis method of the present application can simulate the dynamic smoking process of different smokers according to different smoking habits, oral depth and other parameters, and simulate the different dynamic migration of flavor substances by the artificial lips, which is used for the research on the correlation between smoke components and sensory perception.

[0039] In summary, the present method simulates the dynamic contact and migration of the flavor wrapper by the simulated smoking device. The method is more close to the actual cigarette smoking process, and can provide strong technical support and development basis for the basic researches of cigarette product research and development, smoke physiological perception, smoking and health, etc.

[0040] The basic concept of the present application is as follows:

[0041] When a cigarette consumer smokes a cigarette product, the taste tipping paper directly contacts the human lips. Because there is a small amount of thin layer of saliva on the lips, and because of the processes of clamping, sucking, taking, and spitting the cigarette, the taste tipping paper will rub against the lips. The taste substances will stay on the lips during this process, or because of a small amount of licking, the lips closing action, etc., the taste substances will migrate into the oral cavity, causing a taste sensation. During the smoking process, because of the swallowing of smoke, the saliva swallowing action, etc., the dynamic migration of the taste substances will also be caused, thereby causing the taste experience during the smoking of the smoke. According to this process, the present application designs a bionic simulation device for studying the dynamic migration of the taste substances in the taste tipping paper: when starting to smoke, the cigarette is connected to the artificial lips through the clamp, and contacts the surface thereof. The smoke is introduced into the artificial oral cavity through the pipe, diffused, and sucked into the smoke collector for reservation. After the first mouthful of smoking, the cigarette is taken out, and the smoke in the artificial oral cavity is discharged by adjusting the valve and using the vacuum pump. Then, the subsequent cigarette is inserted, and the subsequent cigarette is smoked by using the smoking machine.

[0042] Compared with the prior art, the present application has the following advantages:

[0043] 1. The present application considers the dynamic process of the tipping paper of the cigarette filter and the mouth (mainly the lips) of the smoker during the simulation of the smoking of the cigarette, and also considers the key processes of the cigarette smoke entering the human mouth and the smoke being discharged. During the smoking process, the taste substances on the tipping paper will migrate, stay, or even transfer because of the insertion and extraction of the filter, which is particularly important for the study of the influence of the taste substances in the tipping paper on the smoking of the cigarette, and is the basis and core of the study of the smoke components and the sensory perception.

[0044] Most of the current researches are still focused on the detection of the total amount of the taste substances in the tipping paper, and there is a lack of corresponding devices and methods for the study of the real-time sensory perception. In view of the fact that there are few reports on the migration behavior of the taste substances in the taste tipping paper during the smoking process, the present application can simulate the smoking process of the taste tipping paper more closely, and pay attention to the possible migration behavior and migration form of the taste substances. More importantly, the artificial oral cavity is connected, the results after the dynamic migration of the taste substances can be monitored at any time, and the present application has strong practicability and operability.

[0045] 2. In summary, the present application considers the actual smoking process, the contact between the filter during the smoking of the cigarette and the lips of the oral cavity, thereby causing the dynamic migration of the taste substances. Therefore, the analysis method of the present application can effectively reflect the real migration of the taste substances.

[0046] 3. In addition, the present application also takes into full account the contact of taste substances with the lips and the entry of part of the taste substances into the oral cavity due to licking or saliva swallowing. Therefore, the present application can effectively simulate the influence of taste tipping paper on the taste of smoke during the smoking of cigarettes.

[0047] 4. The dynamic migration amount of taste substances obtained by the present application has a higher correlation with the sensory quality of cigarettes during smoking. The analysis data obtained by the method are closer to the actual smoking conditions and can better reflect the flavor changes of cigarette products, thereby providing strong technical support for the research and development of cigarette products, the physiological perception of smoke, the relationship between smoking and health, and the like. BRIEF DESCRIPTION OF DRAWINGS

[0048] Figure 1 A schematic diagram of an analysis device for the dynamic migration amount of taste substances in the taste tipping paper used in the embodiments of the present application.

[0049] Figure 2 An analysis chromatogram of the dynamic migration amount of sucralose sweet taste substances in an imported sweet taste tipping paper under the standard smoking condition of Example 1.

[0050] Figure 3 An analysis chromatogram of the dynamic migration amount of aspartame taste substances in an imported sweet taste tipping paper under the deep smoking condition of Example 2.

[0051] Figure 4 An analysis chromatogram of the migration of composite taste substances (a is an analysis chromatogram of the migration amount of simulated lips, and b is an analysis chromatogram of the migration amount of an artificial oral cavity) of Example 3.

[0052] Figure 5 An analysis chromatogram of the dynamic migration amount of taste substances in an imported sweet taste tipping paper under the long-term smoking mode of a cigarette filter rod of Example 4 (a is an analysis chromatogram of the migration amount of artificial lips, and b is an analysis chromatogram of the migration amount of an artificial oral cavity).

[0053] Figure 6 An analysis chromatogram of the dynamic migration amount of sour taste substances in a sour taste tipping paper under the standard smoking condition of Example 5.

[0054] LIST OF REFERENCE NUMERALS

[0055] 1. simulated lips; 2. cigarette holder; 3. simulated oral cavity; 4. smoke collector; 5. sheet of smoke collection and adsorption material; 6. cigarette; 7. taste tipping paper; 8. vacuum pump; 9. smoke outlet; 10. valve; 11. smoking machine or similar smoking device; 12. connecting hose. DETAILED DESCRIPTION

[0056] In order to make the objects, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and should not be used to limit the present application. The raw materials and equipment used in the embodiments, unless specified, can be purchased from the market or commonly used in the art, and the methods in the embodiments, unless specified, are conventional methods in the art.

[0057] Those skilled in the art can understand that the singular forms "a", "an" and "the" used herein include plural forms, unless specifically stated otherwise. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specified. It should be further understood that the phrase "comprising" used in the specification of the present application means that the features, integers, steps, operations, elements and / or components exist, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or groups thereof. It should be understood that when we say an element is "connected" to another element, it can be directly connected to the other element or there can be intermediate elements. In addition, "connected" used herein can include wireless connection.

[0058] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0059] In addition, the terms "first", "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "a plurality of" is two or more, unless otherwise specifically limited.

[0060] In the present application, unless specifically defined and limited otherwise, the terms "mounting", "connected", "connection", "fixed", and the like, should be construed broadly and do not necessarily mean that two elements are directly connected or connected to each other, or that two elements are in contact with each other. In addition, the terms "connected" or "connection" can include an electrically connected, a mechanically connected, or a logically connected, and can include an indirect connection through an intermediate medium. In addition, the terms "connected" or "connection" can include a connection between two elements, or a connection between two elements and another element.

[0061] In the present application, unless specifically defined and limited otherwise, the terms "mounting", "connected", "connection", "fixed", and the like, should be construed broadly and do not necessarily mean that two elements are directly connected or connected to each other, or that two elements are in contact with each other. In addition, the terms "connected" or "connection" can include an electrically connected, a mechanically connected, or a logically connected, and can include an indirect connection through an intermediate medium. In addition, the terms "connected" or "connection" can include a connection between two elements, or a connection between two elements and another element.

[0062] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present application, the illustrative description of the above terms should not be understood as necessarily referring to the same embodiment or example. In addition, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in the present application.

[0063] Those skilled in the art can understand that, unless otherwise defined, all terms used herein, including technical terms and scientific terms, have the same meaning as that generally understood by those skilled in the art to which the present application belongs. It should also be understood that terms such as those defined in a general dictionary should be understood as having a meaning consistent with that in the context of the prior art, and should not be interpreted in an idealized or overly formal sense unless otherwise defined.

[0064] The purpose of the present application is to provide a method for analyzing the dynamic migration amount of taste components in taste paper and a related biomimetic simulation collection device in view of the lack and deficiency of the current method for analyzing the dynamic migration amount of taste substances in taste paper.

[0065] The method can simulate the bionic simulation of the taste of the tipping paper during the cigarette smoking process, including friction, contact, smoke suction, smoke diffusion, etc., and at the same time, the insertion and extraction of each cigarette filter are performed, so as to restore the dynamic process of the taste of the tipping paper during the smoking process to the maximum extent.

[0066] As Figure 1 The bionic simulation collection device of the dynamic migration amount of the taste substance in the taste tipping paper of the present application comprises: a simulation lip 1, a cigarette holder 2, a simulation oral cavity 3, a smoke collector 4, a smoke collection adsorption material sheet 5, a cigarette 6, a taste tipping paper 7, a vacuum pump 8, a smoke exhaust port 9, a first valve 10-1, a second valve 10-2, a third valve 10-3, a smoking machine 11 or a similar smoking device, and a connecting hose 12.

[0067] The simulation lip 1, the simulation oral cavity 3, the smoke collector 4, and the smoking machine 11 are sequentially connected by the connecting hose 12 from upstream to downstream. In the embodiment, the connecting hose 12 is a silica gel hose.

[0068] The simulation oral cavity 3 is also connected to the suction device 8 through the third valve 10-3 and the connecting hose 12, so as to suck the gas in the simulation oral cavity 3 through the suction device 8. The downstream of the suction device 8 is the smoke exhaust port 9.

[0069] The simulation lip 1 has a cigarette holder 2 for holding the cigarette 1, and also has a smoke passage connected to the cigarette holder 2, which is connected to the simulation oral cavity 3 through the connecting hose 12. The cigarette holder 2 is a clamping rod-shaped structure, which does not affect the contact between the cigarette 6 and the simulation lip 1.

[0070] The smoke inlet pipe is provided with a first valve 10-1;

[0071] The simulation oral cavity 3 has a cavity for smoke diffusion, and the simulation oral cavity 7 is connected to the smoke collector 4;

[0072] The downstream end of the smoke collector 4 is connected to the smoking machine 11 through the second valve 10-2;

[0073] The smoke collector 4 is provided with a smoke collection adsorption material sheet 5. The smoke collection adsorption material sheet 5 is arranged in parallel with the cross section of the smoke collector 4. The smoke collection adsorption material sheet 5 is arranged at 90 degrees to the direction of smoke flow, so that the smoke completely passes through the smoke collection adsorption material sheet 5.

[0074] In the embodiment, the simulation lip 1 is a porous hollow columnar structure soaked with artificial saliva. The material of the simulation lip 1 is silica gel sponge, which can soak a thin layer of artificial saliva, and the depth of the oral cavity can be adjusted to 3-10 cm.

[0075] In the embodiment, the cigarette holder 2 is made of elastic silica gel, and the clamping diameter is 5-8 mm, which can be customized according to the size of the cigarette.

[0076] In the embodiment, the simulated oral cavity 3 is a closed cavity with a smoke inlet and an outlet, and the effective contact inner area is about 120-150 cm 2 The material of the simulated oral cavity 3 is glass.

[0077] In the embodiment, the simulated oral mucosa inside the simulated oral cavity 3 is a hydrophilic adsorption material layer, which can be tightly attached to the inside of the simulated oral cavity after being soaked in artificial saliva, and forms a stable liquid film for simulating the saliva layer in the simulated oral cavity.

[0078] The hydrophilic adsorption material layer can be a paper adsorption film, etc., and the thickness can be 0.01-10 mm.

[0079] The smoke collector 4 is a closed cavity with an air inlet and an air outlet, and the smoke collection and adsorption material sheet 5 inside is a Cambridge filter sheet or other adsorption material.

[0080] The method for analyzing the dynamic migration amount of the taste substance in the taste tipping paper of the application is as follows:

[0081] The analysis method includes the following steps using the bionic simulation collection device of the dynamic migration amount of the taste substance in the taste tipping paper of the application:

[0082] a. Place the cigarette 6 into the simulated lip 1 through the cigarette holder 2, and at the same time, slightly press the simulated lip 1 to allow the taste tipping paper 7 to contact the artificial saliva on the simulated lip 1; set the smoking machine 11 with the suction capacity of 35 mL, the suction interval of 1 min, and the suction duration of 2 s;

[0083] b. Soak the simulated lip 1 with the artificial saliva, and soak the inner wall of the simulated oral cavity 3 with the artificial saliva, and if necessary, paste a layer of hydrophilic adsorption material layer on the inner wall as the simulated oral mucosa to adsorb the artificial saliva;

[0084] c. Put the smoke collection and adsorption material sheet 5 into the smoke collector 4, and connect the simulated lip 1, the simulated oral cavity 3, the smoke collector 4, and the smoking machine 11 through the connecting hose 12, the first valve 10-1, and the second valve 10-2; connect the vacuum pump 8 with the simulated oral cavity 3 through the third valve 10-3 and the hose 12;

[0085] d. After all the components are connected, open the first valve 10-1 and the second valve 10-2, and close the third valve 10-3, and check the air tightness;

[0086] Place the cigarette 6 into the simulated lip 1 through the cigarette holder 2, start the smoking machine 11, light the cigarette 6, and start smoking;

[0087] e. After the start of smoking, the first puff of smoke enters the simulated oral cavity 3 through the simulated lips 1, stays for a short time, and is adsorbed by the smoke adsorption material sheet 5 in the smoke collector 4;

[0088] Subsequently, the smoking machine 11 enters an interval waiting time of 60 s, quickly removes the cigarette from the simulated lips 1, closes the first valve 10-1 and the second valve 10-2, opens the valve 10-3, and opens the vacuum pump 8 to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of the smoker spitting smoke;

[0089] f. 5 s before the smoking machine starts the second puff, first open the valve 10-1 to quickly restore the negative pressure in the simulated oral cavity 3, close the third valve 10-3, open the second valve 10-2, and timely insert the cigarette 6, maintain consistent mouth depth, and allow the taste tipping paper 7 to contact the simulated lips 1;

[0090] g. Pull out the cigarette 6, repeat the above steps to exhaust the smoke, and reinsert the cigarette 6, which is completed within 1 min of the puff interval, and the smoking process begins, repeating steps e and f until the end of smoking;

[0091] h. After the end of smoking, remove the simulated lips 1 and flush them with a small amount of artificial saliva multiple times, collect the flushing liquid, and use chromatographic instruments to analyze the composition of the taste substances;

[0092] Collect the artificial saliva in the simulated oral cavity 3 and use artificial saliva to rinse the inner wall of the simulated oral cavity multiple times, combine the rinsing liquid and the original artificial saliva, and use chromatographic instruments to evaluate and analyze the migration amount of taste substances.

[0093] The formula of the artificial saliva in the embodiment is NaCl 0.4 g / L, KCl 0.4 g / L, CaCl2·2H2O 0.795 g / L, NaH2PO4·2H2O 0.78 g / L, Na2S·2H2O 0.005 g / L, urea 1.0 g / L, pH = 6.8 ± 0.5, mainly used for soaking the surface of the simulated lips 1 and the inner wall of the simulated oral cavity 3.

[0094] The following analysis is performed using the above-mentioned bionic simulation collection device for the dynamic migration amount of taste substances in the taste tipping paper.

[0095] Example 1

[0096] Analysis of the dynamic migration amount of sucralose sweet taste substances in sweet tipping paper under standard puffing conditions

[0097] 1. Reagent preparation: a cigarette sample with a sweet taste is placed in an environment with a temperature of 2℃±1℃ and a relative humidity of 60±3% for 48 hours to balance the moisture content, and qualified cigarettes are selected for smoking;

[0098] 2. Device preparation: a thin layer of artificial saliva is applied to the surface of the simulated lips 1, a 120cm 2 long paper adsorption film is inserted into the simulated oral cavity 3 and soaked with artificial saliva, and the paper adsorption film is tightly attached to the inner wall of the simulated oral cavity 3;

[0099] The simulated lips 1, simulated oral cavity 3, smoke collector 4, vacuum pump 8 and smoking machine 11 are connected, and the smoking parameters are set, with a smoking capacity of 35mL, a smoking interval of 1min and a smoking duration of 2s. The first valve 10-1 and the second valve 10-2 connected to the simulated oral cavity 3 and the smoking machine 11 are opened, the third valve 10-3 connected to the vacuum pump 8 is closed, and the air tightness is checked;

[0100] 3. Simulation of the taste of the tipping paper during smoking:

[0101] The cigarette 6 with the taste tipping paper 7 is placed into the simulated lips 1 through the cigarette holder 2, with a mouth depth of 5mm. The smoking machine 11 is started, the cigarette 6 is lit, and smoking begins;

[0102] After smoking begins, the first puff of smoke enters the simulated oral cavity 3 through the simulated lips 1, stays for a short time, and is adsorbed by the Cambridge filter in the smoke collector 4;

[0103] Subsequently, the smoking machine 11 enters an interval waiting time of 60s, the cigarette 6 is quickly removed from the simulated lips 1 and placed next to the device for smoldering. The first valve 10-1 and the second valve 10-2 are closed, the third valve 10-3 is opened, and the vacuum pump 8 is turned on to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of a smoker exhaling smoke;

[0104] Subsequently, 5s before the smoking machine 11 begins the second puff, the first valve 10-1 is opened to quickly restore the negative pressure in the simulated oral cavity 3, the vacuum pump 8 and the third valve 10-3 are closed, the second valve 10-2 is opened, and the cigarette 6 is inserted in time, maintaining a consistent mouth depth and allowing the taste tipping paper 7 to contact the simulated lips 1;

[0105] After the second puff, the cigarette 6 is removed, the smoke is exhausted, and the cigarette 6 is reinserted. This process is completed within 1min of the smoking interval, and the smoking process begins. The above steps of smoking and exhausting smoke are repeated multiple times until the eighth puff is reached, and the smoking process ends. Three cigarettes are repeatedly smoked;

[0106] 4. Taste substance detection: After smoking, the simulated lips 1 were removed and rinsed with artificial saliva for several times, and the rinsing liquid was combined and diluted to 5 mL. The collected liquid was filtered and analyzed by chromatographic instrument. The sweet taste substance migration amount was evaluated by high performance chromatograph with evaporative light scattering detector using the following method. Figure 2 The chromatogram of the sweet taste substance migrated by the simulated lips is shown in the following figure (peak 1 - sucralose).

[0107] The instrument detection method of the sweet taste substance is as follows: chromatographic column: BEH C 18 chromatographic column (2.1 mm x 100 mm, 1.7 μm); mobile phase: A: water, B: HPLC grade acetonitrile, gradient elution program: 0-15.0 min, 10% B-40% B; 15.1-16.0 min, 40% B-10% B; flow rate: 0.20 mL / min, column temperature: 30°C; injection volume: 10.0 μL; evaporative light scattering detector conditions: drift tube temperature: 60°C, nebulizer mode: heating, power level: 100%, carrier gas: nitrogen (purity > 99.9%), carrier gas pressure: 30 psi.

[0108] After analysis, the dynamic migration amount of the sucralose sweet taste substance was 11.3 μg / cig (cigarette).

[0109] Example 2

[0110] Analysis of the dynamic migration amount of sweet taste substance in imported composite sweet taste tipping paper under deep puffing condition

[0111] 1. Reagent preparation: the cigarette samples with composite sweet taste tipping paper were balanced in an environment with temperature 2°C±1°C and relative humidity 60%±3% for 48 h, and the qualified cigarettes were selected for smoking;

[0112] 2. Device preparation: a thin layer of artificial saliva was applied on the surface of the simulated lips 1, a piece of 150 cm 2 long paper adsorption film was inserted into the simulated oral cavity 3 and soaked with artificial saliva, and the paper adsorption film was tightly attached to the inner wall of the simulated oral cavity 3; the simulated lips 1, the simulated oral cavity 3, the smoke collector 4, the vacuum pump 8 and the smoking machine 11 were connected, and the puffing parameters were set, the puffing volume was 55 mL, the puffing interval was 30 s, and the puffing duration was 2 s; the first valve 10-1 and the second valve 10-2 were opened, the third valve 10-3 was closed, and the air tightness was checked;

[0113] 3. Simulated taste tipping paper smoking process: the cigarette 6 with the taste tipping paper 7 was inserted into the simulated lips 1 and fixed on the cigarette holder 2 with a mouth depth of 8 mm; the smoking machine 11 was started, the cigarette 6 was lit, and smoking was started;

[0114] After the first puff, the first puff of smoke passed through the simulated lips 1 into the simulated oral cavity 3 and stayed for a short time, and was adsorbed by the Cambridge filter in the smoke collector 4;

[0115] Subsequently, the smoking machine 11 entered an interval waiting time of 30 s, quickly removed the cigarette 6 from the simulated lips 1 and placed it next to the device for smoldering;

[0116] The first valve 10-1 and the second valve 10-2 were closed, the third valve 10-3 was opened, and the vacuum pump 8 was opened to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of the smoker spitting out smoke;

[0117] Subsequently, 5 s before the smoking machine 11 began the second puff, the first valve 10-1 was first opened to quickly restore the negative pressure in the simulated oral cavity 3, the vacuum pump 8 and the third valve 10-3 were closed, the second valve 10-2 was opened, and the cigarette 6 was inserted in time, maintaining a consistent depth of holding, and the taste of the tipping paper 7 was in contact with the simulated lips 1;

[0118] After the second puff, the cigarette 6 was removed, the smoke was exhausted, and the cigarette 6 was reinserted, all of which were completed within 1 min of the interval between puffs, the smoking process began, and the above steps of smoking and exhausting smoke were repeated multiple times until the fifth puff was smoked, the smoking ended, and the five cigarettes were repeatedly smoked.

[0119] 4. Detection of taste substances: After the smoking ended, the simulated lips 1 were removed and a small amount of artificial saliva was used for multiple rinses, the rinsing liquids were collected and combined, and the volume was made to be 2 mL; the collected liquid was filtered and analyzed for the composition of the taste substances using a chromatographic instrument; the artificial saliva in the simulated oral cavity 3 was collected and used to rinse the inner wall of the simulated oral cavity 3 multiple times, the rinsing liquid and the original artificial saliva were combined, and the amount of sweet taste substance transferred was evaluated and analyzed using a high-performance chromatographic instrument combined with an evaporative light scattering detector. Figure 3 The chromatogram for the transfer of aspartame taste substances to the simulated lips is shown below (peak 1 - aspartame).

[0120] The instrument detection method for sweet taste substances is as follows: chromatographic column: BEH C 18 Chromatographic column (2.1 mm x 100 mm, 1.7 μm); mobile phase: A: 20 mmol / L ammonium acetate water, B: HPLC grade acetonitrile, gradient elution program: 0-15.0 min, 10% B-40% B; 15.1-16.0 min, 40% B-80% B; 16.1-17.0 min, 80% B-10% B; flow rate: 0.20 mL / min, column temperature: 35°C; injection volume: 10.0 μL; PDA detector, detection wavelength: 214 nm.

[0121] After analysis, the dynamic migration amount of the sweet taste substance of aspartame was: 0.89 μg / cig for the simulated lips 1, and no migration was detected for the simulated oral cavity 3, which might be due to the low aspartame content of the tipping paper.

[0122] Example 3

[0123] Analysis of the dynamic migration amount of the taste substance in the composite sweet tipping paper under the standard smoking condition

[0124] 1. Reagent preparation: the cigarette sample with sweet taste tipping paper was balanced in an environment with a temperature of 2°C±1°C and a relative humidity of 60%±3% for 48 hours, and qualified cigarettes were selected for cigarette smoking.

[0125] 2. Device preparation: a thin layer of artificial saliva was applied to the surface of the simulated lips 1, a piece of 140 cm 2 paper adsorption film was inserted into the simulated oral cavity 3 and soaked with artificial saliva, and the paper adsorption film was tightly attached to the inner wall of the simulated oral cavity 3; the simulated lips 1, the simulated oral cavity 3, the smoke collector 4, the vacuum pump 8 and the smoking machine 11 were connected, and the smoking parameters were set, the smoking capacity was 35 mL, the smoking interval was 60 s, and the smoking duration was 2 s; the first valve 10-1 and the second valve 10-2 were opened, the third valve 10-3 was closed, and the air tightness was checked.

[0126] 3. Simulated taste tipping paper smoking process: the cigarette 6 with the taste tipping paper 7 was inserted into the simulated lips 1 and fixed on the cigarette holder 2 with a mouth depth of 6 mm; the smoking machine 11 was started, the cigarette 6 was lit, and smoking was started.

[0127] After smoking started, the first puff of smoke entered the simulated oral cavity 3 through the simulated lips 1, stayed for a short time, and was adsorbed by the Cambridge filter in the smoke collector 4; then, the smoking machine 11 entered the interval waiting time of 60 s, the cigarette 6 was quickly removed from the simulated lips 1 and placed beside the device for smoldering.

[0128] The first valve 10-1 and the second valve 10-2 were closed, the third valve 10-3 was opened, and the vacuum pump 8 was turned on to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of a smoker spitting out smoke.

[0129] Subsequently, 5s before the smoking machine 11 starts the second puff, first open the first valve 10-1, let the negative pressure in the simulated oral cavity 3 quickly recover, close the vacuum pump and the third valve 10-3, open the second valve 10-2, and timely insert the cigarette 6, keep the depth of mouth consistent, and let the taste of the tipping paper 7 contact the simulated lips 1; after the second puff, pull out the cigarette 6, exhaust the smoke, and reinsert the cigarette 6, which is completed within 1min of the puffing interval, the smoking process starts, and the above smoking and smoke exhaust steps are repeated multiple times until the eighth puff is smoked, the smoking ends, and 5 cigarettes are repeatedly smoked;

[0130] 4. Detection of taste substances: after the smoking ends, remove the simulated lips 1, and use artificial saliva to rinse a small amount of multiple times, collect and combine the three times of rinsing liquids, and make up to 5mL; the collected liquid is filtered, and the composition of the taste substances is analyzed by using a chromatographic instrument; the artificial saliva in the simulated oral cavity 3 is collected, and the inner wall of the simulated oral cavity 3 is repeatedly washed with artificial saliva, and the washing liquid and the original artificial saliva are combined, and the evaluation analysis of the migration amount of sweet taste substances is carried out by using a high-performance chromatograph combined with an evaporative light scattering detector. Figure 4 For composite taste substance migration analysis, chromatogram a is the simulated lip migration amount analysis chromatogram, and chromatogram b is the simulated oral cavity migration amount chromatogram, (peak 1-trichlorosucrose, peak 2-aspartame, peak 3-mogroside).

[0131] Sweet taste substance instrument detection method: chromatographic column: BEH C 18 Chromatographic column (2.1mmx100mm, 1.7μm); mobile phase: A: 20mmol / L ammonium acetate water, B: HPLC grade acetonitrile, gradient elution program: 0-15.0min, 10% B-50% B; 15.1-17.0min, 50% B-90% B; 17.1-18.1min, 90% B-10% B; flow rate: 0.25mL / min, column temperature: 35℃; injection volume: 10.0μL; evaporative light scattering detector conditions: drift tube temperature: 65℃, nebulizer mode: heating, power level: 100%, carrier gas: nitrogen (purity >99.9%), carrier gas pressure: 35psi.

[0132] Through analysis, the analysis results of the dynamic migration amount of the composite sweet taste substances are shown in Table 1.

[0133] Table 1 Analysis results of the dynamic migration amount of the composite sweet taste substances

[0134] Sweetener class Simulated lip migration (pg / cig) Simulated oral migration (pg / cig) Sucralose 15.4 2.7 Aspartame 2.2 0.8 Mogroside 1.1 Not detected

[0135] Example 4

[0136] Analysis of the dynamic migration amount of taste substances in an imported sweet tipping paper under a long-term holding cigarette filter rod smoking mode

[0137] 1. Reagent preparation: A sample of cigarette with sweet taste tipping paper was equilibrated for 48 h at a temperature of 2℃±1℃ and a relative humidity of 60±3%, and qualified cigarettes were selected for smoking;

[0138] 2. Device preparation: A thin layer of artificial saliva was applied to the surface of the simulated lips 1, a piece of 150 cm 2 square paper adsorption film was inserted into the simulated oral cavity 2 and immersed in artificial saliva, and the paper adsorption film was tightly attached to the inner wall of the simulated oral cavity 3; the simulated lips 1, the simulated oral cavity 3, the smoke collector 4, the vacuum pump 8 and the smoking machine 11 were connected, and the smoking parameters were set, the smoking capacity was 35 mL, the smoking interval was 60 s, and the smoking duration was 2 s; the first valve 10-1 and the second valve 10-2 were opened, the third valve 10-3 was closed, and the air tightness was checked;

[0139] 3. Simulated taste tipping paper smoking process: the cigarette 6 with the taste tipping paper 7 was inserted into the simulated lips 1 and fixed on the cigarette holder 2, and the depth of mouth holding was 7 mm; the smoking machine 11 was started, the cigarette 6 was lit, and smoking was started;

[0140] After smoking started, the first puff of smoke entered the simulated oral cavity 3 through the simulated lips 1, stayed for a short time, and was adsorbed by the Cambridge filter in the smoke collector 4; then, the smoking machine 11 entered the interval waiting time of 60 s;

[0141] The cigarette 6 was quickly removed from the simulated lips 1 and placed beside the device for smoldering; the first valve 10-1 and the second valve 10-2 were closed, the third valve 10-3 was opened, and the vacuum pump 8 was turned on to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of a smoker spitting out smoke;

[0142] Then, 5 s before the smoking machine 11 started the second puff, the first valve 10-1 was opened first to quickly restore the negative pressure in the simulated oral cavity 3, the vacuum pump and the third valve 10-3 were closed, the second valve 10-2 was opened, and the cigarette 6 was inserted in time, keeping the depth of mouth holding consistent and allowing the taste tipping paper 7 to contact the simulated lips 1;

[0143] After smoking started, the second puff of smoke entered the simulated oral cavity 3 through the simulated lips 1, stayed for a short time, and was adsorbed by the Cambridge filter in the smoke collector 4; then, the smoking machine 11 entered the interval waiting time of 60 s;

[0144] The cigarette 6 was removed, the smoke was exhausted, and the cigarette 6 was reinserted, starting from the third puff, keeping the cigarette filter in the mouth, closing the first valve 10-1, and keeping the smoking machine 11 in the smoking state without smoking the cigarette smoke, so that the third and fourth puffs were sucked under the suction program of the smoking machine 11;

[0145] At the fifth puff, first open the first valve 10-1, close the third valve 10-3, open the second valve 10-2, and timely insert the cigarette 6, keep the puffing depth consistent, and let the taste tipping paper 7 contact the simulated lips 1;

[0146] After the start of smoking, the fifth puff of smoke passes through the simulated lips 1 into the simulated oral cavity 3, stays for a short time, and is adsorbed by the Cambridge filter in the smoke collector 4; then, the smoking machine 11 enters an interval waiting time of 60 s;

[0147] The steps of pulling out the cigarette 6 and discharging smoke are repeated again, then the cigarette filter is kept in the mouth again, the valve 10-1 is closed, and the smoking machine 11 is kept in the state of suction, but does not suck the cigarette smoke. In this way, the sixth, seventh, and eighth puffs are sucked under the suction program of the smoking machine 11, and the eighth puff is ended, the filter is removed, and the above steps are repeated to smoke 5 cigarettes;

[0148] 4. Detection of taste substances: after smoking is finished, the simulated lips 1 are removed, and a small amount of artificial saliva is used for multiple rinsing. The rinsing liquid is collected and combined for three times, and the volume is made to be 5 mL. The collected liquid is filtered, and the composition of the taste substances is analyzed by using a chromatographic instrument. The artificial saliva in the simulated oral cavity 3 is collected, and the inner wall of the simulated oral cavity 3 is repeatedly rinsed with artificial saliva. The rinsing liquid and the original artificial saliva are combined, and the migration amount of the sweet taste substance is evaluated and analyzed by using a high-performance chromatograph combined with an evaporative light scattering detector. Figure 5 The chromatogram of the sweet taste substance (a is the migration amount analysis chromatogram of the simulated lips, and b is the migration amount chromatogram of the simulated oral cavity).

[0149] The instrument detection method of the sweet taste substance is as follows: the chromatographic column is BEH C 18 The chromatographic column is (2.1 mm x 100 mm, 1.7 μm). The mobile phase is A: 20 mmol / L ammonium acetate water and B: HPLC grade acetonitrile. The gradient elution program is 0-15.0 min, 10% B-50% B; 15.1-16.0 min, 50% B-10% B. The flow rate is 0.20 mL / min, the column temperature is 35°C, the injection amount is 10.0 μL, and the PDA detector is used with a detection wavelength of 209 nm.

[0150] Through analysis, the dynamic migration amount of the sweet taste substance mogroside is as follows: the migration amount of the simulated lips is 10.2 μg / cig, and the migration amount of the simulated oral cavity is 1.3 μg / cig.

[0151] Example 5

[0152] Analysis of the dynamic migration amount of the sour taste substance in a certain sour tipping paper under standard puffing conditions

[0153] 1. Reagent preparation: A sample of cigarette with sweet taste tipping paper was equilibrated for 48 h at a temperature of 2℃±1℃ and a relative humidity of 60±3%, and qualified cigarettes were selected for smoking;

[0154] 2. Device preparation: A thin layer of artificial saliva was applied to the surface of the simulated lips 1, and a piece of 120 cm 2 square paper adsorption film was inserted into the simulated oral cavity 3 and soaked with artificial saliva, and the paper adsorption film was tightly attached to the inner wall of the simulated oral cavity 3. The simulated lips 1, simulated oral cavity 3, smoke collector 4, vacuum pump 8 and smoking machine 11 were connected, and the smoking parameters were set, with a smoking capacity of 35 mL, a smoking interval of 1 min and a smoking duration of 2 s. The first valve 10-1 and the second valve 10-2 were opened, the third valve 10-3 was closed, and the air tightness was checked;

[0155] 3. Simulated taste tipping paper smoking process: The cigarette 6 with taste tipping paper 7 was inserted into the simulated lips 1 and fixed on the cigarette holder 2, with a mouth depth of 8 mm. The smoking machine 11 was started, and the cigarette 6 was lit to start smoking;

[0156] After the start of smoking, the first puff of smoke entered the simulated oral cavity 3 through the simulated lips 1 and stayed for a short time, and was adsorbed by the Cambridge filter in the smoke collector 4. Then, the smoking machine 11 entered the interval waiting time of 60 s, and the cigarette 6 was quickly removed from the simulated lips 1 and placed beside the device for smoldering. The first valve 10-1 and the second valve 10-2 were closed, the third valve 10-3 was opened, and the vacuum pump 8 was turned on to exhaust the residual smoke in the simulated oral cavity 3, simulating the behavior of a smoker spitting out smoke;

[0157] Then, 5 s before the smoking machine 11 started the second puff, the first valve 10-1 was opened to quickly restore the negative pressure in the simulated oral cavity 3, the vacuum pump and the third valve 10-3 were closed, the second valve 10-2 was opened, and the cigarette 6 was inserted in time, keeping the mouth depth consistent and allowing the taste tipping paper 7 to contact the simulated lips 1;

[0158] After the second puff, the cigarette 6 was removed, the smoke was exhausted, and the cigarette 6 was reinserted, all of which were completed within 1 min of the smoking interval. The smoking process began, and the above steps of smoking, removing and exhausting the cigarette were repeated until the eighth puff, and the smoking ended. Three cigarettes were repeatedly smoked;

[0159] 4. Detection of taste substances: After smoking, the simulated lips 1 were removed and rinsed with artificial saliva several times, and the three rinsing solutions were combined and diluted to 2 mL. Figure 6 The chromatogram of the acid substances migrated by the simulated lips is shown in the figure, with peak 1 being malic acid and peak 2 being citric acid.

[0160] The instrumental method for detecting the sour substances is as follows: a chromatographic column: BEH C 18 a chromatographic column (2.1 mm x 100 mm, 1.7 μm); a mobile phase: A: 0.1% phosphoric acid aqueous solution (volume percent), B: HPLC grade acetonitrile, gradient: 90% A-10% B, a flow rate is 0.20 mL / min, a column temperature is 30°C; a sample injection amount is 5.0 μL; a PDA detector, a detection wavelength: 210 nm.

[0161] Through analysis, the analysis result of the dynamic migration amount of the sour taste substance simulating the lip migration amount is: 5.5 μg / cig (branch) of malic acid, 3.6 μg / cig (branch) of citric acid.

Claims

1. A biomimetic simulation collection device for the dynamic migration of flavor substances in flavor-sensory packaging paper, characterized in that, The biomimetic simulation collection device includes: a simulated lip (1), a simulated oral cavity (3), a smoke collector (4), and a smoking machine (11) connected sequentially from upstream to downstream; The simulated oral cavity (3) is also connected to the suction device (8) through a third valve (10-3) so that the gas inside the simulated oral cavity (3) can be extracted by the suction device (8); The simulated lips (1) have a smoke channel, which is connected to the simulated oral cavity (3) via a connecting hose (12); The flue gas inlet pipe is equipped with a first valve (10-1); The simulated oral cavity (3) has a cavity for smoke diffusion, and the cavity is connected to the smoke collector (4); The downstream end of the flue gas collector (4) is connected to the smoking machine (11) through a second valve (10-2); The flue gas collector (4) is equipped with a flue gas collection and adsorption material sheet (5).

2. The biomimetic simulation collection device for the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 1, characterized in that, The air extraction device (8) is a vacuum pump.

3. The biomimetic simulation collection device for the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 1, characterized in that, The simulated lips (1) are porous hollow columnar structures soaked in artificial saliva.

4. The biomimetic simulation collection device for the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 1, characterized in that, The inner wall of the simulated oral cavity (7) is provided with a simulated oral mucosa, and the simulated oral mucosa is soaked with artificial saliva.

5. The biomimetic simulation collection device for the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 1, characterized in that, The cigarette holder (2) is an elastic silicone holder used to hold cigarettes of different sizes.

6. The biomimetic simulation collection device for the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 1, characterized in that, The simulated oral cavity (7) is a glass chamber.

7. A method for analyzing the dynamic migration of flavor substances in flavor-sensory packaging paper, characterized in that, Using the biomimetic simulation collection device according to any one of claims 1-6, the analysis method includes: Step a. Place the cigarette (6) with flavored tipping paper (7) into the simulated lips (1) through the cigarette holder (2), while making the flavored tipping paper (7) contact the simulated lips (1); Step b. The simulated lips (1) are evenly moistened with artificial saliva, and the inner wall of the simulated oral cavity (3) is also moistened with artificial saliva; Step c. Place the flue gas collection and adsorption material (5) into the flue gas collector (4), and connect the simulated lips (1), simulated oral cavity (3), flue gas collector (4), and smoking machine (11) through the connecting hose (12) and the first valve (10-1) and the second valve (10-2); The vacuum pump (8) is connected to the simulated oral cavity (3) via the third valve (10-3); Step d. Open the first valve (10-1) and the second valve (10-2), close the third valve (10-3), and check the airtightness; Place the cigarette (6) into the simulated lips (1) through the cigarette holder (2), start the smoking machine (11), light the cigarette (6), and start smoking; Step e. After smoking begins, the first puff of smoke enters the simulated oral cavity (3) through the simulated lips (1) and stays there briefly before being absorbed by the smoke adsorption material (5) as it passes through the smoke collector (4). Subsequently, the smoking machine enters an interval waiting time, removes the cigarette (6) from the simulated lips (1), closes the first valve (10-1) and the second valve (10-2), opens the third valve (10-3), and turns on the suction device (8) to expel the residual smoke in the simulated oral cavity (3), simulating the smoker's exhalation behavior; Step f. Five seconds before the smoking machine starts the second puff, first open the first valve (10-1) to quickly restore the negative pressure in the simulated oral cavity (3), close the third valve (10-3), open the second valve (10-2), and insert the cigarette (6) in time, keep the depth of the cigarette (6) in the mouth consistent with the previous one, and let the flavor-sensing paper (7) contact the simulated lips (1); Step g. Repeat steps e and f multiple times until the smoking is finished; Step h. After smoking, remove the simulated lips (1) and rinse with artificial saliva. Collect the rinsing liquid for compositional analysis of taste substances. Artificial saliva was collected from the simulated oral cavity (3), and the inner wall of the simulated oral cavity (3) was rinsed multiple times with the artificial saliva. The rinsing solution and the original artificial saliva collected from the simulated oral cavity (3) were combined to evaluate and analyze the migration amount of taste substances.

8. The method for analyzing the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 7, characterized in that, A layer of hydrophilic adsorption material is attached to the inner wall of the simulated oral cavity (3) to simulate the adsorption of artificial saliva by the oral mucosa.

9. The method for analyzing the dynamic migration of flavor substances in flavor-sensory packaging paper according to claim 7, characterized in that, When analyzing the dynamic migration of flavor substances in flavor-sensory tipping paper under long-term oral cigarette filter use, the following modification was made during a repetition of step ef: Keep the cigarette filter in your mouth and keep the flavor-sensing paper (7) in contact with the simulated lips (1); close the first valve (10-1), and the smoking machine (11) will keep in the sucking state, but will not suck the cigarette smoke; in this way, take one or several puffs through the sucking program set by the smoking machine (11).

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