Congjiang Yao bath lotion for gynecological health care as well as preparation method and application of Congjiang Yao bath lotion

Abstract

The invention relates to a Congjiang Yao bath lotion for gynecological health care and a preparation method and application thereof, and belongs to the technical field of ethnic medicine modernization. The Congjiang Yao bath lotion for gynecological health care is prepared from the following medicinal raw materials by mass: 50 to 70 g of semiliquidambar cathayensis, 50 to 70 g of uncaria rhynchophylla, 50 to 70 g of sarcandra glabra, 30 to 40 g of basil, 80 to 100 g of motherwort, 20 to 30 g of dandelion, 30 to 50 g of golden cypress, 20 to 30 g of wintercreeper and 30 to 50 g of radix sophorae flavescentis. The motherwort herb is fed in two batches. According to the traditional Chinese medicine composition, the Yao medicine ternary theory, the traditional Chinese medicine monarch, minister, assistant and guide theory and the modern pharmacology theory are combined, the wind medicine, the spraying medicine, the drilling medicine, the wind dispelling and dampness eliminating medicine, the blood circulation promoting and blood stasis removing medicine and the anti-inflammation and antibacterial medicine are combined, the mutual complementing and compounding synergistic effect is achieved, and the traditional Chinese medicine composition has the remarkable gynecological health care effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of modernization of ethnic medicine, in particular to a Congjiang Yao bath liquid for gynecological health care and a preparation method and application thereof. BACKGROUND

[0002] The most characteristic of the Yao nationality is the medicinal bath therapy, also known as the Pangtong medicinal bath. A variety of herbs are decocted and used to soak the whole body or a local part, which has the effects of dispelling wind and dampness, promoting blood circulation to remove meridian obstruction, strengthening the body, preventing and treating skin diseases, and is most famous for the treatment of postpartum recovery and rheumatic bone pain. Congjiang Yao bath has been listed as a national intangible cultural heritage, and its "three breaks and three cleans" theory is widely used in the field of gynecology. The most famous Yao medicinal bath is "three postpartum baths", which is also known as "three-day work". "Three postpartum baths" is to add characteristic Yao medicine to the warm bath and use it to bathe the new mother, so as to prevent and treat postpartum symptoms. The first postpartum bath is to prevent postpartum wind and tetanus, the purpose is to stop bleeding and pain, anti-inflammatory and dredge collaterals, enhance uterine contraction and accelerate lochia discharge; the second bath is to prevent wound infection and inflammation at the injury site and regulate organ function; the third bath is to nourish yin and assist yang, restore vitality, and focus on improving the immunity of the puerpera and preventing various gynecological diseases. This medicinal bath therapy is called "three postpartum baths" by the Yao people. There is also a saying of "three postpartum baths". The first bath removes odors, removes impurities, and reduces lochia; the second bath shrinks yin, contracts the abdomen, and repairs the damaged vagina; the third bath restores physical strength and improves immunity.

[0003] The formula composition of the three postpartum baths varies from place to place, from person to person, and the drug composition is different. Yao doctors are good at dialectical treatment, and decide the compatibility of drugs according to the gain and loss state of the puerpera's body, pay attention to the complementary and balanced effects of different drugs, and do not harm the body of the puerpera. The traditional "monthly medicinal bath" uses "wind medicine" to dispel wind and dampness, "punch medicine" to dispel blood stasis and relieve pain, and "drill medicine" to dredge collaterals and stagnation, which can significantly reduce postpartum lochia, relieve chronic pelvic pain, and regulate menstruation.

[0004] However, the existing folk prescriptions have problems such as unclear medicinal material origin, rough process, uncontrollable quality, etc., and it is difficult to meet the needs of modern women for safety, convenience and standardization. Therefore, there is an urgent need for a gynecological health care Yao bath liquid with clear medicinal material origin, standardized process, definite curative effect and suitable for industrial production. SUMMARY

[0005] In order to solve the above technical problems, the present application provides a Congjiang Yao bath liquid for gynecological health care and a preparation method and application thereof. The present application takes the "wind-punch-drill" three-element compatibility theory of Yao medicine as the core, the formula of Congjiang Yao bath liquid specially used for gynecological health care, the industrialized preparation method thereof and the application thereof in female daily health care, postpartum recovery and auxiliary nursing of chronic pelvic inflammation.

[0006] The present application is realized by the following technical scheme:

[0007] The first objective of this invention is to provide a Congjiang Yao bath liquid for gynecological health care. The medicinal materials of the Congjiang Yao bath liquid, by weight, include: 50-70 g of *Symplocos buergeriana*, 50-70 g of *Uncaria rhynchophylla*, 50-70 g of *Gynostemma pentaphyllum*, 30-40 g of *Gynostemma pentaphyllum*, 80-100 g of *Leonurus japonicus*, 20-30 g of *Taraxacum mongolicum*, 30-50 g of *Phellodendron chinense*, 20-30 g of *Euonymus fortunei*, and 30-50 g of *Sophora flavescens*. The *Leonurus japonicus* is added in two batches, with each batch containing half of the herb.

[0008] An explanation of the "Three-Element Combination" theory in Yao medicine:

[0009] According to the Yao medicine's three-element formula theory, the principal herbs in this prescription are: *Symplocos buergerianum*, *Uncaria rhynchophylla*, and *Gnaphalium affine*. *Symplocos buergerianum* is the premier "wind-dispelling herb," ​​primarily used to dispel wind and dampness, stop leukorrhea and itching; *Gnaphalium affine* is the representative "dispelling herb," ​​primarily used to disperse blood stasis, relieve pain, reduce swelling and dissipate nodules; *Uncaria rhynchophylla* (Yao medicine name "Qin Diao Bei") is a superior "drilling herb," ​​primarily used to open the orifices, extinguish wind, calm the mind, and relieve pain. The three herbs combined form the classic Yao medicine "wind-dispelling-drilling" framework, working together to dispel wind and disperse blood stasis, unblock the meridians, and relieve pain, thus treating leukorrhea, dysmenorrhea, and postpartum blood stasis.

[0010] The assistant herbs are *Spatholobus suberectus* (chicken blood vine) and *Leonurus japonicus* (motherwort). *Spatholobus suberectus* nourishes and invigorates blood, relaxes muscles and tendons; *Leonurus japonicus* is an important gynecological herb that invigorates blood, regulates menstruation, promotes diuresis, and reduces swelling. Together with the principal herbs, they enhance the effects of removing blood stasis and regulating mens

[0011] The adjuvant herbs are dandelion, phellodendron bark, and sophora flavescens, which can clear heat and detoxify, dry dampness and relieve itching, and inhibit common gynecological pathogens (Staphylococcus aureus, Escherichia coli, and Candida albicans).

[0012] The guiding herb is *Fufangteng*, which guides the medicine into the uterus, effectively traversing the Chong and Ren meridians. It also has the functions of calming the fetus, relieving pain, and harmonizing the effects of other herbs.

[0013] The second objective of this invention is to provide a method for preparing the Congjiang Yao bath liquid for gynecological health care, comprising the following steps:

[0014] S1. After cleaning and selecting the raw medicinal materials, coarsely crush them and sieve them;

[0015] S2. Mix half maple, uncaria, and nine-joint wind, add purified water, extract dynamically in a circulating manner, filter, and concentrate the supernatant under reduced pressure to obtain concentrate A.

[0016] S3. Mix the herbs *Gnaphalium affine*, dandelion, phellodendron bark, *Euonymus fortunei*, *Sophora flavescens*, and half of *Leonurus japonicus*, add ethanol for the first reflux extraction, and filter out the first supernatant; add the remaining half of *Leonurus japonicus* and ethanol for the second extraction, and filter out the second supernatant; combine the first and second supernatants, concentrate, and obtain concentrate B.

[0017] S4. Combine the obtained concentrate A and concentrate B, add chitosan for flocculation and clarification, let stand, centrifuge at high speed, and take the supernatant.

[0018] S5. Add purified water to the supernatant obtained in step S4, adjust the pH to acidic, filter to remove bacteria, and fill to obtain Congjiang Yao bath liquid for gynecological health care.

[0019] In one embodiment of the present invention, in step S2, the half maple, uncaria, and nine-joint wind are added with purified water at a mass-volume ratio of 1:10-15;

[0020] And / or, in step S2, the temperature of the dynamic cyclic extraction is 80℃~95℃, and the time of the dynamic cyclic extraction is 6 h~8 h.

[0021] In one embodiment of the present invention, in step S2, the vacuum concentration is carried out to a relative density of 1.05 g / mL to 1.10 g / mL.

[0022] In one embodiment of the present invention, in step S3, the temperature of the first reflux extraction is 70°C to 85°C, and the time of the first reflux extraction is 3 h to 4 h;

[0023] And / or, the temperature of the second reflux extraction is 70℃~85℃, and the time of the second reflux extraction is 3 h~4 h.

[0024] In one embodiment of the present invention, in step S3, the concentration is carried out to a relative density of 1.05 g / mL to 1.10 g / mL.

[0025] In one embodiment of the present invention, in step S4, the added chitosan has a mass percentage of 0.3%.

[0026] In one embodiment of the present invention, in step S5, the mass ratio of the supernatant to the purified water is 1:1.0-1:1.5.

[0027] In one embodiment of the present invention, in step S5, the pH value of the acid is 5.0-6.0.

[0028] The third objective of this invention is to provide the application of the Congjiangyao bath liquid for gynecological health care in the preparation of drugs for promoting lochia discharge, relieving uterine contraction pain, chronic pelvic inflammatory disease, or dysmenorrhea.

[0029] The usage method is as follows:

[0030] 1. Daily health care: Take 50 mL of this product and add it to warm water at 40℃~42℃ (the water volume should be enough to submerge the upper edge of the pubic symphysis). Take a sitz bath or a full-body bath for 15 min~20 min, 2~3 times a week.

[0031] 2. After 7 days postpartum: Once a day for 20 minutes each time, for 7 consecutive days, to promote lochia discharge and relieve uterine contraction pain;

[0032] 3. Chronic pelvic inflammatory disease or dysmenorrhea: Start using on the 3rd day after menstruation ends, once a day for 20 minutes each time, for 10 consecutive days as one course of treatment.

[0033] The technical solution of the present invention has the following advantages compared with the prior art:

[0034] 1. This invention provides a Yao bath liquid from Congjiang for gynecological health care, its preparation method, and its application. This invention combines the Yao medicine's three-element theory, the traditional Chinese medicine's theory of principal, assistant, and adjuvant herbs, and modern pharmacological theories (…). Figure 1 This formula combines wind-dispelling, dampness-removing, blood-activating, and anti-inflammatory drugs with other herbs, resulting in a synergistic effect and significant benefits for gynecological health.

[0035] 2. This invention optimizes the extraction process to obtain a formula with stable content of effective ingredients and significant effects.

[0036] 3. The efficacy of the bath liquid has been verified through clinical cases. Attached Figure Description

[0037] To make the content of this invention easier to understand, the invention will be further described in detail below with reference to specific embodiments and accompanying drawings, wherein...

[0038] Figure 1 This is a schematic diagram of the Yao medicine "wind-striking-drilling" ternary formula of the present invention;

[0039] Figure 2 This invention uses an ultrasound image of the uterus before using the Jiangyao Bath Health Formula;

[0040] Figure 3 This is an ultrasound image of the uterus after using the Jiangyao Bath Health Formula according to the present invention. Detailed Implementation

[0041] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, so that those skilled in the art can better understand and implement the present invention. However, the embodiments described are not intended to limit the present invention.

[0042] This invention provides a gynecological health care product from Congjiang Yao, which, by weight, is composed of the following medicinal materials: 50-70 g of *Symplocos buergeriana*, 50-70 g of *Uncaria rhynchophylla*, 50-70 g of *Sarcandra glabra*, 30-40 g of *Spatholobus suberectus*, 80-100 g of *Leonurus japonicus* (added in two batches), 20-30 g of *Taraxacum mongolicum*, 30-50 g of *Phellodendron chinense*, 20-30 g of *Euonymus fortunei*, and 30-50 g of *Sophora flavescens*.

[0043] This invention also provides a preparation process suitable for industrial production, comprising the following steps:

[0044] 1. Cleaning and crushing: After cleaning all medicinal materials, coarsely crush them to pass through a 10-mesh sieve;

[0045] 2. Group extraction:

[0046] 1) "Three-Element Herbal Extracts": Half-Maple Leaf Lotus, Uncaria Rhizome, and Nine-Jointed Wind are added with purified water at a mass-to-volume ratio of 1:10-15. The mixture is dynamically extracted at 80℃-95℃ for 6-8 hours. After filtration, the supernatant is concentrated under reduced pressure to a relative density of 1.05 g / mL-1.10 g / mL (60℃) to obtain concentrated solution A.

[0047] 2) "Assistant and Adjuvant Group": The remaining medicinal materials were added to 20% ethanol at a mass-to-volume ratio of 1:8-10, and extracted by reflux at 70℃-85℃ for 3-4 hours, twice; the motherwort was divided into two equal parts by mass, one part was added for the first extraction, and the other part was added for the second extraction; the two extracts were combined, the ethanol was recovered until there was no alcohol odor, and concentrated to a relative density of 1.05 g / mL-1.10 g / mL (60℃) to obtain concentrated solution B;

[0048] 3. Merging and purification: Combine concentrates A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant;

[0049] 4. Preparation and sterilization: Add purified water to adjust the total amount to 1 to 1.5 times the total amount of medicinal materials, adjust the pH to 5.0 to 6.0, filter to sterilize, and fill to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0050] The relative density was measured directly using a common densitometer.

[0051] Example 1

[0052] This embodiment provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, as detailed below:

[0053] The ingredients of the Congjiang Yao bath liquid used for gynecological health care in this embodiment are: 50 g of half maple leaf, 50 g of uncaria, 50 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort, 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0054] The preparation method of Congjiang Yao bath liquid for gynecological health care in this embodiment is as follows:

[0055] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind, add 1.5L of purified water, extract dynamically at 90℃ for 6 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0056] (2) Mix nine-layered wind, dandelion, phellodendron bark, euonymus fortunei, sophora flavescens and 40 g of motherwort. For the first extraction, add 1.36 L of 20% ethanol and reflux at 80℃ for 3 h. Filter out the supernatant. Add another 40 g of motherwort to the residue for the second extraction. Add 1.68 L of 20% ethanol and extract for 3 h. Filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol taste, and concentrate at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution B.

[0057] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; preparation and sterilization: add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 6.0, filter to sterilize, and fill to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0058] Example 2

[0059] This embodiment provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, as detailed below:

[0060] The raw materials for the Congjiang Yao bath liquid used in this embodiment for gynecological health care are: 70 g of half maple leaf, 70 g of uncaria, 70 g of nine-jointed wind, 40 g of nine-layered wind, 100 g of motherwort (added in two batches, 50 g each), 30 g of dandelion, 50 g of phellodendron bark, 30 g of Euonymus fortunei, and 50 g of Sophora flavescens.

[0061] The preparation method of Congjiang Yao bath liquid for gynecological health care in this embodiment is as follows:

[0062] (1) After cleaning all the medicinal materials, mix and coarsely crush them until they pass through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind, add 2.1 L of purified water and extract dynamically at 90℃ for 6 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0063] (2) Mix the herbs, including *Gnaphalium affine*, *Leonurus japonicus* (50 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, and *Sophora flavescens*, add 2 L of 20% ethanol, and reflux at 80°C for 3 h. Filter out the supernatant. Add the other half of *Leonurus japonicus* (50 g) to the residue, add 2.4 L of 20% ethanol, and reflux at 80°C for 3 h. Filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrate B.

[0064] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 5.0, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0065] Example 3

[0066] This embodiment provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, as detailed below:

[0067] The raw materials for the Congjiang Yao bath liquid used in this embodiment for gynecological health care are: 70 g of half maple leaf, 70 g of uncaria, 70 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort (added in two batches), 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0068] The preparation method of Congjiang Yao bath liquid for gynecological health care in this embodiment is as follows:

[0069] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 3.15 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0070] (2) Add 1.7 L of 20% ethanol to the following herbs: *Gnaphalium affine*, *Leonurus japonicus* (40 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, *Sophora flavescens*, and the remaining herbs. Reflux at 75°C for 4 h and filter out the supernatant. Add 40 g of *Leonurus japonicus* to the residue and then add 1.74 L of 20% ethanol. Reflux at 75°C for 4 h and filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrate B.

[0071] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.3 times the total amount of medicinal materials, adjust the pH to 6.0, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0072] Example 4

[0073] This embodiment provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, as detailed below:

[0074] The raw materials for the Congjiang Yao bath liquid used in this embodiment for gynecological health care are: 50 g of half maple leaf, 50 g of uncaria, 50 g of nine-jointed wind, 40 g of nine-layered wind, 100 g of motherwort (added in two batches), 30 g of dandelion, 50 g of phellodendron bark, 30 g of euonymus fortunei, and 50 g of sophora flavescens.

[0075] The preparation method of Congjiang Yao bath liquid for gynecological health care in this embodiment is as follows:

[0076] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 2.25 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, concentrate under reduced pressure at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0077] (2) Add 2.5 L of 20% ethanol to the residue of the herb *Leonurus japonicus*, *Leonurus japonicus* (50 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, and *Sophora flavescens*, and reflux at 85°C for 4 h. Filter the supernatant. Add the other half of *Leonurus japonicus* (50 g) to the residue, add 2.55 L of 20% ethanol, and reflux at 85°C for 4 h. Filter the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrated solution B.

[0078] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 5.5, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0079] Comparative Example 1

[0080] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, similar to Example 1, except that: 30 g of *Symplocos buergeriana* is included in the raw materials; details are as follows:

[0081] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 30 g of half maple leaf, 50 g of uncaria, 50 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort, 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0082] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0083] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind, add 1.3L of purified water, extract dynamically at 90℃ for 6 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0084] (2) Mix nine-layered wind, dandelion, phellodendron bark, euonymus fortunei, sophora flavescens and 40 g of motherwort. For the first extraction, add 1.36 L of 20% ethanol and reflux at 80℃ for 3 h. Filter out the supernatant. Add another 40 g of motherwort to the residue for the second extraction. Add 1.68 L of 20% ethanol and extract for 3 h. Filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol taste, and concentrate at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution B.

[0085] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; preparation and sterilization: add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 6.0, filter to sterilize, and fill to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0086] Comparative Example 2

[0087] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 1, except that: 30 g of Uncaria rhynchophylla is included in the raw materials; details are as follows:

[0088] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 50 g of half maple leaf, 30 g of uncaria, 50 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort, 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0089] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0090] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind, add 1.3L of purified water, extract dynamically at 90℃ for 6 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0091] (2) Mix nine-layered wind, dandelion, phellodendron bark, euonymus fortunei, sophora flavescens and 40 g of motherwort. For the first extraction, add 1.36 L of 20% ethanol and reflux at 80℃ for 3 h. Filter out the supernatant. Add another 40 g of motherwort to the residue for the second extraction. Add 1.68 L of 20% ethanol and extract for 3 h. Filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol taste, and concentrate at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution B.

[0092] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; preparation and sterilization: add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 6.0, filter to sterilize, and fill to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0093] Comparative Example 3

[0094] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 1, except that: 30 g of *Gynostemma pentaphyllum* is included in the raw materials; details are as follows:

[0095] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 50 g of half maple leaf, 50 g of uncaria, 30 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort, 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0096] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0097] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind, add 1.3L of purified water, extract dynamically at 90℃ for 6 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0098] (2) Mix nine-layered wind, dandelion, phellodendron bark, euonymus fortunei, sophora flavescens and 40 g of motherwort. For the first extraction, add 1.36 L of 20% ethanol and reflux at 80℃ for 3 h. Filter out the supernatant. Add another 40 g of motherwort to the residue for the second extraction. Add 1.68 L of 20% ethanol and extract for 3 h. Filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol taste, and concentrate at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution B.

[0099] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; preparation and sterilization: add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 6.0, filter to sterilize, and fill to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0100] Comparative Example 4

[0101] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 2, except that the motherwort is added only once in the preparation method; details are as follows:

[0102] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 70 g of half maple leaf, 70 g of uncaria, 70 g of nine-jointed wind, 40 g of nine-layered wind, 100 g of motherwort, 30 g of dandelion, 50 g of phellodendron bark, 30 g of euonymus fortunei, and 50 g of sophora flavescens.

[0103] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0104] (1) After cleaning all the medicinal materials, mix and coarsely crush them until they pass through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind, add 2.1 L of purified water and extract dynamically at 90℃ for 6 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0105] (2) Mix the herbs, including *Gnaphalium affine*, *Leonurus japonicus*, *Taraxacum mongolicum*, *Phellodendron chinense*, *Euonymus fortunei*, and *Sophora flavescens*, add 2L of 20% ethanol, reflux at 80℃ for 3 h, filter out the supernatant, add 2.4L of 20% ethanol to the residue, reflux at 80℃ for 3 h, filter out the supernatant, combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, concentrate at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL, to obtain concentrated solution B;

[0106] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 5.0, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0107] Comparative Example 5

[0108] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 3, except that the extraction temperature in step (1) is 70℃; specifically as follows:

[0109] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 70 g of half maple leaf, 70 g of uncaria, 70 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort (added in two batches), 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0110] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0111] (1) After cleaning all the medicinal materials, crush them into coarse pieces and pass them through a 10-mesh sieve; mix half maple, uncaria and nine-joint wind and add 3.15 L of purified water for dynamic circulation extraction at 70℃ for 8 h, filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0112] (2) Add 1.7 L of 20% ethanol to the following herbs: *Gnaphalium affine*, *Leonurus japonicus* (40 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, *Sophora flavescens*, and the remaining herbs. Reflux at 75°C for 4 h and filter out the supernatant. Add 40 g of *Leonurus japonicus* to the residue and then add 1.74 L of 20% ethanol. Reflux at 75°C for 4 h and filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrate B.

[0113] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.3 times the total amount of medicinal materials, adjust the pH to 6.0, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0114] Comparative Example 6

[0115] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 3, except that the reflux extraction temperature in step (2) is 65℃; specifically as follows:

[0116] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 70 g of half maple leaf, 70 g of uncaria, 70 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort (added in two batches), 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0117] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0118] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 3.15 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0119] (2) Add 1.7 L of 20% ethanol to the following herbs: *Gnaphalium affine*, *Leonurus japonicus* (40 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, *Sophora flavescens*, and the remaining herbs. Reflux at 65°C for 4 h and filter out the supernatant. Add 40 g of *Leonurus japonicus* to the residue and then add 1.74 L of 20% ethanol. Reflux at 65°C for 4 h and filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrate B.

[0120] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.3 times the total amount of medicinal materials, adjust the pH to 6.0, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0121] Comparative Example 7

[0122] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 3, except that the reflux extraction temperature in step (2) is 90℃; specifically as follows:

[0123] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 70 g of half maple leaf, 70 g of uncaria, 70 g of nine-jointed wind, 30 g of nine-layered wind, 80 g of motherwort (added in two batches), 20 g of dandelion, 30 g of phellodendron bark, 20 g of euonymus fortunei, and 30 g of sophora flavescens.

[0124] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0125] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 3.15 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, and concentrate the supernatant at 60℃ under reduced pressure to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0126] (2) Add 1.7 L of 20% ethanol to the following herbs: *Gnaphalium affine*, *Leonurus japonicus* (40 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, *Sophora flavescens*, and the remaining herbs. Reflux at 90°C for 4 h and filter out the supernatant. Add 40 g of *Leonurus japonicus* to the residue and then add 1.74 L of 20% ethanol. Reflux at 90°C for 4 h and filter out the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrate B.

[0127] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.3 times the total amount of medicinal materials, adjust the pH to 6.0, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0128] Comparative Example 8

[0129] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 4, except that: in step (2), it is concentrated to a relative density of 1.20; specifically as follows:

[0130] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 50 g of half maple leaf, 50 g of uncaria, 50 g of nine-jointed wind, 40 g of nine-layered wind, 100 g of motherwort (added in two batches), 30 g of dandelion, 50 g of phellodendron bark, 30 g of euonymus fortunei, and 50 g of sophora flavescens.

[0131] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0132] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 2.25 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, concentrate under reduced pressure at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0133] (2) Add 2.5 L of 20% ethanol to the residue of the herb *Leonurus japonicus*, *Leonurus japonicus* (50 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, and *Sophora flavescens*, and reflux at 85°C for 4 h. Filter the supernatant. Add the other half of *Leonurus japonicus* (50 g) to the residue, add 2.55 L of 20% ethanol, and reflux at 85°C for 4 h. Filter the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol taste, and concentrate at 60°C to a relative density of 1.20 to obtain concentrate B.

[0134] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 5.5, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0135] Comparative Example 9

[0136] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 4, except that 0.5% chitosan is added for flocculation and clarification in step (3); as follows:

[0137] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 50 g of half maple leaf, 50 g of uncaria, 50 g of nine-jointed wind, 40 g of nine-layered wind, 100 g of motherwort (added in two batches), 30 g of dandelion, 50 g of phellodendron bark, 30 g of euonymus fortunei, and 50 g of sophora flavescens.

[0138] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0139] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 2.25 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, concentrate under reduced pressure at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0140] (2) Add 2.5 L of 20% ethanol to the residue of the herb *Leonurus japonicus*, *Leonurus japonicus* (50 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, and *Sophora flavescens*, and reflux at 85°C for 4 h. Filter the supernatant. Add the other half of *Leonurus japonicus* (50 g) to the residue, add 2.55 L of 20% ethanol, and reflux at 85°C for 4 h. Filter the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrated solution B.

[0141] (3) Combine concentrated liquid A and B, add 0.5% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 5.5, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0142] Comparative Example 10

[0143] This comparative example provides a Congjiang Yao bath liquid for gynecological health care and its preparation method, which is similar to Example 4, except that the pH value is adjusted to 7 in step (3); as follows:

[0144] The ingredients of the Congjiang Yao Bath Liquid used in this comparative study for gynecological health care are: 50 g of half maple leaf, 50 g of uncaria, 50 g of nine-jointed wind, 40 g of nine-layered wind, 100 g of motherwort (added in two batches), 30 g of dandelion, 50 g of phellodendron bark, 30 g of euonymus fortunei, and 50 g of sophora flavescens.

[0145] The preparation method of Congjiang Yao bath liquid used in this comparative example for gynecological health care is as follows:

[0146] (1) After cleaning all the medicinal materials, coarsely crush them to pass through a 10-mesh sieve; mix half maple, uncaria, and nine-joint wind, add 2.25 L of purified water, and extract dynamically at 85℃ for 8 h. Filter, concentrate under reduced pressure at 60℃ to a relative density of 1.05 g / mL~1.10 g / mL to obtain concentrated solution A;

[0147] (2) Add 2.5 L of 20% ethanol to the residue of the herb *Leonurus japonicus*, *Leonurus japonicus* (50 g), dandelion, *Phellodendron chinense*, *Euonymus fortunei*, and *Sophora flavescens*, and reflux at 85°C for 4 h. Filter the supernatant. Add the other half of *Leonurus japonicus* (50 g) to the residue, add 2.55 L of 20% ethanol, and reflux at 85°C for 4 h. Filter the supernatant. Combine the supernatants from the two extractions, recover the ethanol until there is no alcohol odor, and concentrate at 60°C to a relative density of 1.05 g / mL to 1.10 g / mL to obtain concentrated solution B.

[0148] (3) Combine concentrated liquid A and B, add 0.3% chitosan for flocculation and clarification, let stand at 4℃ for 12 h, centrifuge at high speed, and take the supernatant; add purified water to adjust the total amount to 1.5 times the total amount of medicinal materials, adjust the pH to 7, filter to remove bacteria, and fill into bottles to obtain the finished product of Congjiang Yao bath liquid for gynecological health care.

[0149] Test Example 1: Content Detection Method

[0150] 1. Gallic acid and its detection method

[0151] Semi-leaved lily (Liriope muscari) is an endangered medicinal plant endemic to China. Its roots, leaves, and nectar are widely used in folk medicine for dispelling wind and dampness, promoting blood circulation, and relieving inflammation and pain. The natural polyphenol gallic acid it contains has antioxidant properties (3-5 times that of vitamin C), anti-aging effects, skin-protecting properties, antibacterial and anti-inflammatory effects (inhibiting pathogenic bacteria such as Staphylococcus aureus and Escherichia coli; reducing inflammatory factors such as IL-6 and TNF-α), and hemostatic and astringent properties (promoting platelet aggregation, constricting blood vessels, and reducing exudate). It can be used to treat oral ulcers, skin infections, eczema, etc.

[0152] The method for detecting gallic acid by high performance liquid chromatography is as follows:

[0153] Chromatographic column: ZORBAX SB-C18 4.6×150 mm, 5 μm; mobile phase: A is 0.5% acetic acid in water (v / v), B is acetonitrile, gradient elution, program as follows:

[0154] Table 1 Elution procedure for HPLC detection of gallic acid

[0155]

[0156] Flow rate: 1.0 mL / min; column temperature: 35°C; injection volume: 10 μL; detection wavelength: 280 nm.

[0157] The following table shows the gallic acid content in the Yao bath products obtained from various formulas / processes:

[0158] Table 2. Amount of gallic acid in different examples and comparative products

[0159]

[0160] As can be seen from the table above, the gallic acid content in Examples 1-4 is all higher than 1000 mg / L; the amount of *Ligustrum lucidum* used in Comparative Example 1 is significantly lower than the range of the invention, so the gallic acid content is only over 600 mg / L; the amount of *Ligustrum lucidum* used in Comparative Examples 2-4 is within the range of the invention, but the gallic acid content is still lower than 1000 mg / L, indicating that different drug combinations have a synergistic effect, which may be related to the dissolution and extraction of gallic acid; Comparative Examples 5-10 have different extraction, concentration, precipitation, and storage conditions, which, as can be seen from the table above, will also significantly affect the yield of gallic acid in the finished product, thus affecting the efficacy of the product.

[0161] 2. Rhododendronine and its detection methods

[0162] Uncaria rhynchophylla is used medicinally with its dried, hooked stems and branches. Its medicinal effects, such as "lowering blood pressure, calming the nerves, relieving convulsions, and protecting the brain," come from a multi-component system with indole alkaloids as its core, including rhynchophylline, isorhynchophylline, dehydrorhynchophylline, and rhynchophylline.

[0163] The method for detecting rhynchophylline by high performance liquid chromatography is as follows:

[0164] Column: CAPCELL PAK C18, 4.6 × 250 mm, 5 μm;

[0165] Mobile phase: acetonitrile-0.1% triethylamine aqueous solution (pH adjusted to 9.0 with phosphoric acid), gradient elution 0-25 min, acetonitrile 15%-35%; flow rate: 1.0 mL / min;

[0166] Column temperature: 30℃;

[0167] Detection wavelength: 245 nm.

[0168] Table 3. Amount of rhododendronine in products from different embodiments

[0169]

[0170] As can be seen from the table above, the content of rhynchophylline in Examples 1-4 is all higher than 50 mg / L; the amount of rhynchophylline used in Comparative Example 2 is significantly lower than the range of the invention, so the content of rhynchophylline is only more than 30 mg / L; the amount of rhynchophylline used in Comparative Examples 1, 3, and 4 is within the range of the invention, but the content of rhynchophylline is still lower than 50 mg / L, indicating that different drug combinations have a synergistic effect, which may be related to the fact that it helps the rhynchophylline to dissolve and be extracted; Comparative Examples 5-10 have different extraction, concentration, precipitation, and storage conditions, which, as can be seen from the table above, will also significantly affect the yield of rhynchophylline in the finished product, thereby affecting the efficacy of the product.

[0171] 3. Isoazine and its detection methods

[0172] *Sarcandra glabra* (also known as *Sarcandra glabra*) is a plant belonging to the genus *Sarcandra* in the family Chloranthaceae. It is widely used for clearing heat and detoxifying, reducing swelling and relieving pain, and for antibacterial and antiviral purposes. Its active ingredient is isopyridine, a coumarin derivative. Isocyanidin has clear anti-inflammatory, antibacterial, and antiviral effects, and exhibits broad-spectrum inhibitory activity against common pathogenic bacteria such as *Staphylococcus aureus*, *Escherichia coli*, and *Streptococcus*. The pharmacopoeia includes a detection method for isopyridine.

[0173] Chromatographic column: C18 column (250 mm × 4.6 mm, 5 μm);

[0174] Mobile phase: Acetonitrile: 0.1% phosphoric acid = 20:80; Flow rate: 1 mL / min;

[0175] Detection wavelength: 344 nm;

[0176] Column temperature: 30℃;

[0177] Injection volume: 10 μL.

[0178] Table 4. Amount of isozymine terpinene contained in products of different embodiments

[0179]

[0180] As can be seen from the table above, the content of isozyridine in Examples 1-4 is all higher than 50 mg / L; the amount of *Anatomalacia quinquefolia* used in Comparative Example 3 is significantly lower than the range of the invention, so the content of isozyridine is only more than 30 mg / L; the amount of *Anatomalacia quinquefolia* used in Comparative Examples 1, 2, and 4 is within the range of the invention, but the content of isozyridine is still lower than 50 mg / L, indicating that different drug combinations have a synergistic effect, which may be related to the fact that it helps dissolve and extract isozyridine; Comparative Examples 5-10 have different extraction, concentration, precipitation, and storage conditions, which, as can be seen from the table above, will also significantly affect the yield of isozyridine in the finished product, thereby affecting the efficacy of the product.

[0181] 4. Matrine and its detection methods

[0182] Sophora flavescens has a wide range of pharmacological activities. Its main active ingredients are alkaloids, especially matrine and oxymatrine. Sophora flavescens can clear heat and dry dampness, inhibit the accumulation of damp heat, and is used to treat jaundice, leukorrhea, eczema, and vulvar pruritus. It also has insecticidal and antipruritic properties, and is antifungal and antitrichomonal, and is used to treat scabies, vaginitis, and pruritus.

[0183] The pharmacopoeia includes the detection method for matrine:

[0184] Chromatographic column: C18 column (250 mm × 4.6 mm, 5 μm);

[0185] Mobile phase: Acetonitrile: 0.1% phosphoric acid = 20:80; Flow rate: 1 mL / min;

[0186] Detection wavelength: 220 nm;

[0187] Column temperature: 30℃;

[0188] Injection volume: 10 μL.

[0189] Table 5. Amount of matrine in products from different embodiments

[0190]

[0191] As can be seen from the table above, the matrine content in Examples 1-4 is all higher than 800 mg / L, which is the basis for the antibacterial and anti-inflammatory effects of Yao Yu gynecological health care formula. In Comparative Examples 1-5, factors such as low dosage of the principal herb, one-time addition of motherwort, and changes in the extraction temperature of the principal herb, although seemingly unrelated to matrine, also led to a lower matrine content in the finished product. In particular, in Comparative Examples 6-10, the unsuitable extraction temperature of the auxiliary herbs, the unsuitable concentration ratio of the auxiliary herbs, the unsuitable dosage of the precipitant chitosan, and the unsuitable pH of the finished product significantly reduced the matrine content of the finished product, thus affecting the efficacy of the product.

[0192] 5. Leonurine and its detection methods

[0193] Motherwort is a key herb in traditional Chinese medicine gynecology, known for its effects on menstruation, blood circulation, uterine stimulation, anti-inflammation, and antibacterial properties. Its main active ingredients include alkaloids, flavonoids, diterpenes, volatile oils, and coumarins. The detection method for leonurine, listed in the pharmacopoeia, is as follows:

[0194] Chromatographic column: C18 column (250 mm × 4.6 mm, 5 μm);

[0195] Mobile phase: A is 10 mmol / L KH2PO4 (pH 3.0), B is acetonitrile, and the gradient elution program is as follows:

[0196] Table 6 Elution Procedure for Leonuridine HPLC Analysis

[0197]

[0198] Flow rate: 1 mL / min;

[0199] Detection wavelength: 275 nm;

[0200] Column temperature: 30℃;

[0201] Injection volume: 10 μL.

[0202] Table 7. Amount of Leonurine in Products from Different Examples

[0203]

[0204] As can be seen from the table above, the content of leonurine in Examples 1-4 is all higher than 100 mg / L, which is the material basis for the gynecological efficacy of Yao Bath Gynecological Health Formula. In Comparative Examples 1-10, factors such as low dosage of principal ingredient, changes in extraction temperature of principal ingredient, unsuitable extraction temperature of assistant ingredient, unsuitable concentration ratio of assistant ingredient, unsuitable dosage of chitosan precipitant, and unsuitable pH of finished product all lead to lower leonurine content in the finished product. In particular, in Comparative Example 4, since leonurine was added only once and other dosages and process conditions were not changed, the leonurine content in the finished product was significantly reduced, thus affecting the efficacy of the product.

[0205] Test Case 2: Clinical Observation

[0206] To verify the clinical efficacy and safety of the Congjiang Yaoyu gynecological health care formula provided by this invention for postpartum women's physical recovery, the focus was on evaluating its impact on the following aspects: uterine involution (duration of lochia, observation via transabdominal ultrasound) and relief of postpartum pain (abdominal pain, back pain). Healthy full-term women aged 20-35 years with normal delivery were selected, who voluntarily participated and signed informed consent forms, and excluded those with severe skin diseases, allergies, uncontrolled postpartum hemorrhage, severe infections or fever, mental illness or cognitive impairment, or those who refused to participate or could not cooperate with treatment. Sample 2 from Example 2 was used for bathing, once daily for 20 minutes, starting 30 days postpartum, for 7 consecutive days.

[0207] No adverse reactions such as skin allergies, burns, or infections occurred after the postpartum bath, and the vital signs (blood pressure, heart rate, and body temperature) remained stable and normal. The postpartum woman reported significant relief from postpartum pain (verified by VAS score) and improved sleep (verified by PSQI score). An abdominal ultrasound showed... Figure 2 , Figure 3 As shown, before involution, the endometrium showed obvious heterogeneous thickening, which later returned to normal.

[0208] Test Example 3: Antibacterial Experiment

[0209] The diameter of the inhibition zone of this product against common gynecological pathogens (Staphylococcus aureus, Escherichia coli, and Candida albicans) was determined by the agar diffusion method to verify its antibacterial ability.

[0210] Staphylococcus aureus (ATCC 25923) was cultured on MH agar medium, Escherichia coli (ATCC 25922) on MH agar medium, and Candida albicans (ATCC 10231) on SD agar medium. The suspension concentration of Staphylococcus aureus and Escherichia coli was 1~2×10⁻⁶. 8 CFU / mL, Candida albicans adjusted to 1×10⁻⁶ using a hemocytometer. 6 Spores / mL.

[0211] The samples from the examples and comparative examples were filtered through a 0.22 μm microporous membrane to obtain sterile stock solutions. The solutions were then used in experiments and cultured until clear inhibition zones were visible to the naked eye. The diameter of the inhibition zones was measured using a digital caliper cross-section method, and the results were compared based on the average diameter of the inhibition zones (mm, mean ± SD, n=3) (see Table 8 below).

[0212] Table 8 Results of the inhibition zone test

[0213]

[0214] As can be seen from the table above, the average diameter of the antibacterial zone in Examples 1-4 is greater than 20 mm, indicating a strong antibacterial effect. This is the material basis for the gynecological efficacy of Yao Yu gynecological health care formula. In the other formulas, factors such as low dosage of principal ingredient, changes in extraction temperature of principal ingredient, unsuitable extraction temperature of assistant ingredient, unsuitable concentration ratio of assistant ingredient, unsuitable dosage of chitosan precipitant, and unsuitable pH of finished product can all lead to a weakening of the antibacterial effect of the finished product, thereby affecting the efficacy of the product.

[0215] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A bath solution for gynecological care from the Jiangyao bath, characterized in that, The medicinal material raw materials of the Cengjiang Yao bath liquid by mass include: Semen Sterculiae Lychnophorae 50 g-70 g, Uncariae Ramulus et Uncus 50 g-70 g, Saururus chinensis 50 g-70 g, Radix Ilicis Rotundae 30 g-40 g, Leonurus japonicus 80 g-100 g, Herba Taraxaci 20 g-30 g, Phellodendri Cortex 30-50 g, Periploca forrestii 20 g-30 g, Sophora flavescens 30 g-50 g; the Leonurus japonicus is fed in two batches.

2. The method for preparing the Cungjiang Yao bath solution for gynecological care according to claim 1, characterized in that, The method comprises the following steps: S1, after the medicinal material raw materials are cleaned and selected, they are coarsely crushed and sieved; S2, Semen Sterculiae Lychnophorae, Uncariae Ramulus et Uncus and Saururus chinensis are mixed and added with purified water, dynamic circulation extraction is carried out, filtration is carried out, the supernatant obtained is concentrated under reduced pressure to obtain concentrated liquid A; S3, Radix Ilicis Rotundae, Herba Taraxaci, Phellodendri Cortex, Periploca forrestii and half of Leonurus japonicus are mixed, ethanol is added for first reflux extraction, the first supernatant is filtered out; the obtained residue is added with the other half of Leonurus japonicus and ethanol for second extraction, the second supernatant is filtered out; the first supernatant and the second supernatant are combined and concentrated to obtain concentrated liquid B; S4, the concentrated liquid A and the concentrated liquid B are combined, chitosan is added for flocculation and clarification, standing is carried out, high-speed centrifugation is carried out, and the supernatant is taken; S5, the supernatant obtained in step S4 is mixed with purified water, the pH value is adjusted to be acidic, sterilization is carried out by filtration, and filling is carried out to obtain the Cengjiang Yao bath liquid for gynecological health care.

3. The production method according to claim 2, characterized by, In step S2, the Semen Sterculiae Lychnophorae, Uncariae Ramulus et Uncus and Saururus chinensis are added with purified water in a mass-volume ratio of 1:10-15; And / or, in step S2, the temperature of the dynamic circulation extraction is 80℃-95℃, and the time of the dynamic circulation extraction is 6 h-8 h.

4. The production method according to claim 2, characterized by, In step S2, the concentrated liquid is concentrated under reduced pressure to a relative density of 1.05 g / mL-1.10 g / mL.

5. The preparation method according to claim 2, characterized in that, In step S3, the temperature of the first reflux extraction is 70℃-85℃, and the time of the first reflux extraction is 3 h-4 h; And / or, the temperature of the second reflux extraction is 70℃-85℃, and the time of the second reflux extraction is 3 h-4 h.

6. The preparation method according to claim 2, characterized in that, In step S3, the concentrated liquid is concentrated to a relative density of 1.05 g / mL-1.10 g / mL.

7. The preparation method according to claim 2, characterized in that, In step S4, the added mass percentage of chitosan is 0.3%.

8. The preparation method according to claim 2, characterized in that, In step S5, the mass ratio of the supernatant to the purified water is 1:1.0-1:1.

5.

9. The preparation method according to claim 2, characterized in that, In step S5, the pH value of the acid is 5.0-6.

0.

10. The use of the Cengjiang Yao bath liquid for gynecological health care in claim 1 in the preparation of a medicine for promoting lochia discharge, relieving uterine contraction pain, chronic pelvic inflammatory disease or dysmenorrhea.

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