Method for scale-up cultivation of gynoietry fish based on low-salinity acclimation and partition feeding
By using low-salinity acclimatization ponds and a combined light-fluid stress acclimatization process, along with zoned feeding and an intelligent feed conversion system, the problem of salinity adaptation and rapid acclimatization of mandarin fish under artificial conditions has been solved, achieving high survival rates and green production, making it suitable for large-scale breeding of mandarin fish.
Patent Information
- Application Number
- CN202511844200.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-09
- Publication Date
- 2026-03-17
- Estimated Expiration
- 2045-12-09
AI Technical Summary
Existing technologies have failed to effectively address the issues of salinity adaptation, rapid acclimatization, and precise feeding of mandarin fish under artificial conditions, resulting in low survival rates and severely hindering industrial development.
The system employs a low-salinity acclimatization pond combined with a light-flow composite stress acclimatization process, zoned feeding and an intelligent feed conversion system, combined with graded water quality control and micro-ecological stability construction, an AI camera system for monitoring uneaten feed and precise feeding, and modular disease early warning and traditional Chinese medicine prevention and control.
It shortened the acclimatization period, improved the survival rate, reduced the occurrence of diseases, and realized the large-scale, standardized and green production of mandarin fish fry, significantly increasing the number of fry produced per unit of water and the feed utilization rate.
Abstract
Description
Technical Field
[0001] This application relates to the field of aquaculture technology, and in particular to a method for large-scale breeding of spotted mandarin fish fry based on low-salt domestication and zoned feeding. Background Technology
[0002] The spotted mandarin fish (Siniperca scherzeri) belongs to the order Perciformes and the family Perciformes. It is a high-end freshwater economic fish species endemic to my country. Because spotted mandarin fish feed on live prey throughout their lives, they have extremely low acceptance of dead bait and formulated feed under artificial conditions, resulting in a survival rate of less than 30%, which severely restricts their industrial development. However, existing mandarin fish farming methods focus on pond cultivation and live feeding of common mandarin fish, failing to address key issues such as salinity adaptation, rapid acclimatization, and precise feeding. Summary of the Invention
[0003] To address the shortcomings of existing technologies, this application provides a method for large-scale breeding of spotted mandarin fish based on low-salt acclimatization and zoned feeding, which can solve key problems such as salinity adaptation, rapid acclimatization, and precise feeding of spotted mandarin fish.
[0004] To address the aforementioned problems, this application provides a method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding, comprising:
[0005] Provide low-salinity acclimatization ponds;
[0006] The spotted mandarin fish fry were released into the low-salinity acclimatization pond;
[0007] Based on the light-flow composite stress training process, a zoned feeding and intelligent feed conversion system was used to switch the diet of spotted mandarin fish fry;
[0008] After the spotted mandarin fish fry complete their transition to a new diet, graded water quality control and micro-ecological stability are implemented until the spotted mandarin fish fry are harvested and transferred to adult fish farming.
[0009] Furthermore, in the method for large-scale breeding of spotted mandarin fish based on low-salinity acclimatization and zoned feeding provided in this application, the low-salinity acclimatization pond is a rectangular cement pond with a single pond area of 800-1200 m². 2 The pool is 1.6-1.8m deep with a 3% slope at the bottom, featuring a sludge collection trough. The pool walls are lined with nanoporous oxygenation pipes and equipped with a 1.5kW Roots blower; or / and,
[0010] The influent to the low-salinity acclimatization tank is adjusted to a salinity of 3-5‰, a hardness of 180-220 mg / L, and a pH of 7.4-7.8 after sedimentation, sand filtration, and ultraviolet sterilization; or / and,
[0011] The low-salinity acclimatization pond is equipped with an adjustable LED light source and a waterwheel-type flow pump on the top of the pond to form a circulation of 0.2-0.3 m / s.
[0012] Furthermore, in the method for large-scale breeding of spotted mandarin fish fry based on low-salinity acclimatization and zoned feeding provided in this application, the light-current combined stress acclimatization process includes: during the transition feeding period, the aeration should be turned off daily from 18:00 to 20:00 to reduce dissolved oxygen to 4-4.5 mg / L, while simultaneously turning on 200 lx of blue light, forming a 0.4 m / s circulation in the low-salinity acclimatization pond for 20 minutes to induce a stress feeding response in the spotted mandarin fish; or / and,
[0013] After the spotted mandarin fish fry complete their transition to a new diet, graded water quality control and the establishment of a stable micro-ecological environment are implemented until the fry are harvested and transferred to adult fish farming, including:
[0014] During the initial 0-15 days, apply a compound microecological preparation at a concentration of 1.5 mg / L, and supplement with brown sugar at a concentration of 0.5 mg / L every other day, while maintaining ammonia nitrogen ≤ 0.2 mg / L and nitrite ≤ 0.05 mg / L; or / and,
[0015] During the 16-30 day period in the middle stage, the salinity was reduced at a daily gradient of 0.3‰ until the salinity in the low salinity acclimatization pond dropped below 0.5‰, while sodium bicarbonate was added to maintain alkalinity ≥80mg / L.
[0016] During the later 31-45 days, the water transparency in the low salinity acclimatization pond should be controlled between 35-40cm, dissolved oxygen ≥6mg / L and temperature 26-28℃.
[0017] After 45-50 days, the spotted mandarin fish fry with an average total length of ≥6cm, weight of ≥3g, and survival rate of ≥88% are released from the pond and transferred to adult fish farming.
[0018] Furthermore, in the method for large-scale breeding of spotted mandarin fish based on low-salt domestication and zoned feeding provided in this application, the compound microecological preparation includes Bacillus subtilis, lactic acid bacteria and photosynthetic bacteria;
[0019] Among them, Bacillus subtilis ≥1×10 10 cfu / g, lactic acid bacteria ≥5×10 9 cfu / g, photosynthetic bacteria ≥1×10 9 cfu / mL.
[0020] Furthermore, in the method for large-scale breeding of spotted mandarin fish based on low-salinity acclimatization and zoned feeding provided in this application, the low-salinity acclimatization pond is equipped with a floating raft live bait area and an underwater net cage pellet feed area.
[0021] Among them, the floating raft live bait area accounts for 20% of the area of the low salinity acclimatization pond. The zoned feeding and intelligent bait conversion system releases live loach or mud carp with a body length of 50-60% of the total length of the mandarin fish as live bait in the floating raft live bait area. The density ratio of mandarin fish to live bait is 1:(8-10).
[0022] The underwater net cage pellet area accounts for 80% of the area of the low salinity acclimatization pond. The zoned feeding and intelligent feed conversion system includes a double-layer conical acclimatization net cage, which is suspended in the underwater net cage pellet area. The inner layer of the double-layer conical acclimatization net cage is 40 mesh and the outer layer is 20 mesh. Slow-sinking soft pellets with a particle size of 2-4 mm are placed in the double-layer conical acclimatization net cage. The slow-sinking soft pellets contain ≥50% crude protein, ≥10% fat, and 0.4% attractant.
[0023] Furthermore, in the method for large-scale breeding of spotted mandarin fish based on low-salt domestication and zoned feeding provided in this application, the zoned feeding and intelligent feed conversion system also includes an AI camera system, which includes an underwater 4K camera, an edge computing module and a 4G wireless transmission module.
[0024] The AI camera system identifies the area of uneaten feed in real time. When the uneaten feed area is >5%, the feeding amount is automatically reduced by 10%; when it is <1%, it is increased by 5%. Feeding is conducted three times a day, at 07:00, 12:00, and 18:00, with feeding amounts of 40%, 30%, and 30% of the daily ration, respectively. The AI camera system's uneaten feed identification accuracy is ≥95%, and the feeding adjustment delay is <30 seconds. When the pellet feed intake rate is greater than or equal to 60% for two consecutive days, it is determined that the mandarin fish have completed their transition to a new diet. The zoned feeding and intelligent feed conversion system then closes the live bait area on the floating raft. Or / and
[0025] The slow-sinking soft pellet feed comprises the following components by weight: 30% Antarctic krill meal, 25% imported fish meal, 15% fermented soybean meal, 8% enzymatically hydrolyzed squid paste, 12% high-gluten flour, 4% fish oil, 2% lecithin, 3% compound vitamins and mineral salts, 0.4% mandarin fish-specific attractant, and 0.6% sodium alginate; or / and,
[0026] The particle size of the slow-settling soft granules is 2-4 mm, and the stability of the slow-settling soft granules in water is ≥30 min.
[0027] Furthermore, in the method for large-scale breeding of spotted mandarin fish based on low-salt domestication and zoned feeding provided in this application, an early warning and control system is used during the large-scale breeding of spotted mandarin fish fry. The early warning and control system includes an immune enhancement module, a parasite early warning module, and a bacterial disease control module.
[0028] The immune enhancement module is configured to apply 2 mg / L of Astragalus polysaccharide-glycyrrhizic acid compound every 10 days;
[0029] The parasite early warning module is configured to use an infrared camera to photograph the fish's body surface between 02:00 and 04:00 every day. When AI identifies that the area of parasite attachment is ≥1%, 0.3 mg of eucalyptus oil-matrine mixture will be immediately sprayed.
[0030] The bacterial disease control module is configured to respond when the concentration of Vibrio in the water is ≥10. 3 When the cfu / mL level is high, automatically feed the feed containing 2% andrographolide in pellets for 3 consecutive days.
[0031] Furthermore, in the method for large-scale breeding of spotted mandarin fish based on low-salt domestication and zoned feeding provided in this application, the astragalus polysaccharide-glycyrrhizic acid compound agent includes astragalus polysaccharide, glycyrrhizic acid and solvent;
[0032] The mass ratio of Astragalus polysaccharide to glycyrrhizic acid is 3:1. The solvent includes physiological saline, which accounts for 0.9% of the mass of the Astragalus polysaccharide-glycyrrhizic acid complex. The pH of the Astragalus polysaccharide-glycyrrhizic acid complex is 6.8-7.2.
[0033] The method for large-scale breeding of spotted mandarin fish fry based on low-salinity acclimatization and zoned feeding provided in this application involves providing low-salinity acclimatization ponds and releasing spotted mandarin fish fry into them. Simultaneously, a zoned feeding and intelligent feed conversion system is used to transition the spotted mandarin fish fry to a new diet. After the fry have completed this transition, graded water quality control and micro-ecological stability are implemented until the fry are harvested and transferred to adult fish farming. This method solves the industrialization pain points of spotted mandarin fish fry, such as difficulty in transitioning to a new diet, low survival rate, and frequent disease outbreaks. It provides a complete technical solution for the large-scale, standardized, and green production of large-sized spotted mandarin fish fry, and has broad prospects for promotion and significant economic, social, and ecological benefits. Detailed Implementation
[0034] The technical solutions in the embodiments of this application are described clearly and completely below. Obviously, the described embodiments are only some, not all, of the embodiments of this application. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0035] It should be understood that, when used in this specification and the appended claims, the terms "comprising" and "including" indicate the presence of the described features, integrals, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integrals, steps, operations, elements, components and / or collections thereof.
[0036] It should also be understood that the terminology used in this specification is for the purpose of describing particular embodiments only and is not intended to limit the scope of the application. As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.
[0037] It should also be further understood that the term “and / or” as used in this application specification and the appended claims means any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.
[0038] In related technologies, such as Chinese invention patent CN103875571A, a method for large-scale breeding of mandarin fish fry is provided. This method focuses on the breeding of ordinary mandarin fish in ponds and feeding with live bait. However, it does not solve key problems such as the salinity adaptation, rapid acclimatization and precise feeding of spotted mandarin fish.
[0039] Therefore, this application provides a method for large-scale breeding of spotted mandarin fish based on low-salt acclimatization and zoned feeding, which includes:
[0040] Provide low-salinity acclimatization ponds;
[0041] Mandarin fish fry were released into a low-salinity acclimatization pond for polyculture.
[0042] Based on the light-flow composite stress training process, a zoned feeding and intelligent feed conversion system was used to switch the diet of spotted mandarin fish fry;
[0043] After the spotted mandarin fish fry complete their transition to a new diet, graded water quality control and micro-ecological stability are implemented until the spotted mandarin fish fry are harvested and transferred to adult fish farming.
[0044] In this application, by constructing a salinity acclimatization pond, implementing graded water quality control and micro-ecological stability, and setting up a zoned feeding and intelligent feed conversion system, a light-flow composite stress acclimatization process is adopted, along with machine vision-based residual feed monitoring and precise feeding. In addition, modular early disease warning and traditional Chinese medicine prevention and control are also employed. Compared with existing mandarin fish breeding technologies, this application innovatively utilizes 3-5‰ low salinity water to enhance the osmotic pressure regulation ability of spotted mandarin fish, shortening the acclimatization period to 5-7 days. At the same time, a dual-zone feeding system of floating rafts on the water and net cages on the water is adopted to achieve seamless conversion between live feed, soft particles, and hard particles. The average survival rate is ≥88%, the number of fry per unit water volume is increased by 30%, and the feed coefficient is significantly reduced, solving the technical bottlenecks such as difficulty in switching to new feed and low survival rate of spotted mandarin fish.
[0045] In some embodiments, the low-salinity acclimatization pond is a rectangular cement pond with a single pond area of 800-1200 m². 2The pool is 1.6-1.8m deep with a 3% slope at the bottom, featuring a sludge collection trough. The pool walls are lined with nanoporous aeration pipes and equipped with a 1.5kW Roots blower. The influent to the low-salinity acclimatization pool is adjusted to a salinity of 3-5‰, a hardness of 180-220mg / L, and a pH of 7.4-7.8 after sedimentation, sand filtration, and UV sterilization. An adjustable LED light source and a waterwheel-type flow pump are installed on the top of the low-salinity acclimatization pool to create a circulation of 0.2-0.3m / s. The adjustable range of the LED light source is 500-1500lx.
[0046] In this application, the low-salinity acclimatization ponds can be constructed at a mandarin fish farming base in southern China. Ten low-salinity acclimatization ponds with dimensions of 40m x 25m x 1.7m (length x width x depth) will be built, with each pond having an area of 800-1200m². 2 The pool is 1.6-1.8m deep with a 3% slope at the bottom, and a sludge collection trough is installed at the bottom of the slope. The pool walls are equipped with nanoporous oxygenation pipes and a 1.5kW Roots blower. The influent to the low salinity acclimatization pool is adjusted to a salinity of 3-5‰, a hardness of 180-220mg / L, and a pH of 7.4-7.8 after sedimentation, sand filtration, and ultraviolet sterilization. An adjustable LED light source (500-1500lx) and a waterwheel-type flow pump are installed on the top of the pool to form a circulation of 0.2-0.3m / s.
[0047] In some embodiments, the light-flow combined stress acclimatization process includes: during the transition period of mandarin fish fry, the aeration should be turned off from 18:00 to 20:00 every day to reduce dissolved oxygen to 4-4.5 mg / L, while the blue light is turned on at 200 lx, and a circulation of 0.4 m / s is formed in the low salinity acclimatization pond for 20 minutes to induce the mandarin fish to produce a stress feeding response, thereby improving the transition efficiency.
[0048] In some embodiments, after the spotted mandarin fish fry have completed their transition to a new diet, graded water quality control and microecological stability are implemented until the spotted mandarin fish fry are harvested and transferred to adult fish farming, including:
[0049] During the initial 0-15 days, apply a compound microecological preparation at a concentration of 1.5 mg / L, and supplement with brown sugar at a concentration of 0.5 mg / L every other day, while maintaining ammonia nitrogen ≤ 0.2 mg / L and nitrite ≤ 0.05 mg / L; or / and,
[0050] During the 16-30 day period in the middle stage, the salinity was reduced at a daily gradient of 0.3‰ until the salinity in the low salinity acclimatization pond dropped below 0.5‰, while sodium bicarbonate was added to maintain alkalinity ≥80mg / L.
[0051] During the later 31-45 days, the water transparency in the low salinity acclimatization pond should be controlled between 35-40cm, dissolved oxygen ≥6mg / L and temperature 26-28℃.
[0052] After 45-50 days, the spotted mandarin fish fry with an average total length of ≥6cm, weight of ≥3g, and survival rate of ≥88% are released from the pond and transferred to adult fish farming.
[0053] In some embodiments, the compound microecological preparation includes Bacillus subtilis, lactic acid bacteria, and photosynthetic bacteria; wherein, Bacillus subtilis ≥ 1 × 10⁻⁶. 10 cfu / g, lactic acid bacteria ≥5×10 9 cfu / g, photosynthetic bacteria ≥1×10 9 cfu / mL.
[0054] In some embodiments, the low-salinity acclimatization pond includes a floating raft live bait area and an underwater net cage pellet feed area. The floating raft live bait area occupies 20% of the pond's area. A zoned feeding and intelligent feed conversion system releases live loach or gudgeon, with a body length of 50-60% of the total length of the mandarin fish, as live bait in the floating raft live bait area. The density ratio of mandarin fish to live bait is 1:(8-10). The underwater net cage pellet feed area occupies the remaining area of the low-salinity acclimatization pond. The system comprises 80% of the area, and the zoned feeding and intelligent feed conversion system includes a double-layered conical training net cage. The double-layered conical training net cage is suspended in the pellet feed area of the underwater net cage. The inner layer of the double-layered conical training net cage is 40 mesh and the outer layer is 20 mesh. Slow-sinking soft pellets with a particle size of 2-4 mm are placed inside the double-layered conical training net cage. The slow-sinking soft pellets contain ≥50% crude protein, ≥10% fat, and 0.4% attractant.
[0055] In some embodiments, the zoned feeding and intelligent bait conversion system includes an AI camera system, which includes an underwater 4K camera, an edge computing module, and a 4G wireless transmission module. The AI camera system identifies the area of uneaten bait in real time. When the area of uneaten bait is >5%, the feeding amount is automatically reduced by 10%; when it is <1%, it is increased by 5%. Feeding is carried out three times a day, at 07:00, 12:00, and 18:00, with feeding amounts of 40%, 30%, and 30% of the daily ration, respectively. The uneaten bait identification accuracy of the AI camera system is ≥95%, and the feeding adjustment delay is <30s. When the pellet feed consumption rate is greater than or equal to 60% for two consecutive days, it is determined that the mandarin fish fry have completed the transition to a new diet, and the zoned feeding and intelligent bait conversion system closes the live bait area on the floating raft.
[0056] In this application, an AI camera system is installed on the top of the pond to achieve machine vision-based monitoring of uneaten feed and precise feeding. The AI camera system can identify the area of uneaten feed in real time. When the area of uneaten feed is >5%, the feeding amount is automatically reduced by 10%; when it is <1%, it is increased by 5%. Feeding is carried out three times a day, at 07:00, 12:00 and 18:00, with feeding amounts of 40%, 30% and 30% of the daily ration, respectively. At the same time, the uneaten feed identification accuracy of the AI camera system is ≥95% and the feeding adjustment delay is <30s. When the pellet feed consumption rate is greater than or equal to 60% for 2 consecutive days, it is determined that the mandarin fish have completed the transition to other feeds, and the zoned feeding and intelligent feed conversion system closes the live bait area on the floating raft.
[0057] In some embodiments, the slow-sinking soft pellet feed comprises the following components by weight: 30% Antarctic krill powder, 25% imported fish meal, 15% fermented soybean meal, 8% enzymatically hydrolyzed squid paste, 12% high-gluten flour, 4% fish oil, 2% lecithin, 3% compound vitamin and mineral salts, 0.4% mandarin fish attractant, and 0.6% sodium alginate.
[0058] It should be noted that domestically produced fishmeal can also be used for slow-sinking soft granular feed, and the choice can be made according to the actual application. This application does not make any specific restrictions.
[0059] In some embodiments, the particle size of the slow-settling soft granules is 2-4 mm, and the stability of the slow-settling soft granules in water is ≥30 min.
[0060] In some embodiments, during the large-scale breeding of spotted mandarin fish fry, an early warning and control system is employed. This system includes an immune enhancement module, a parasite early warning module, and a bacterial disease control module. The immune enhancement module is configured to apply 2 mg / L of astragalus polysaccharide-glycyrrhizic acid compound every 10 days. The parasite early warning module is configured to use an infrared camera to photograph the fish's body surface between 02:00 and 04:00 daily; if AI identifies a parasite attachment area ≥1%, immediately apply 0.3 mg / L of eucalyptus oil-matrine mixture. The bacterial disease control module is configured to apply a solution when the Vibrio concentration in the water is ≥10... 3 When the cfu / mL level is high, automatically feed the feed containing 2% andrographolide in pellets for 3 consecutive days.
[0061] In some embodiments, the astragalus polysaccharide-glycyrrhizic acid complex comprises astragalus polysaccharide, glycyrrhizic acid, and a solvent; wherein the mass ratio of astragalus polysaccharide to glycyrrhizic acid is 3:1, the solvent comprises physiological saline, the physiological saline accounts for 0.9% of the mass of the astragalus polysaccharide-glycyrrhizic acid complex, and the pH of the astragalus polysaccharide-glycyrrhizic acid complex is 6.8-7.2.
[0062] Compared with the prior art, this application has the following advantages:
[0063] 1. The domestication period is significantly shortened, and the survival rate is significantly improved.
[0064] Using a 3-5‰ low salinity acclimatization pond, the transition of mandarin fish from live bait to formulated feed can be completed in 5-7 days, which is more than 65% shorter than the traditional method (15-20 days). With the help of a dual-zone feeding system of "floating rafts on the water + net cages on the water", the average total length of 45-day-old fish is ≥6cm and the weight is ≥3g, with a survival rate of more than 88%, which is nearly 3 times higher than the conventional breeding (survival rate of about 30%).
[0065] 2. The number of fry produced per unit of water body and the feed efficiency are improved simultaneously.
[0066] The precision feeding system (AI visual monitoring + automatic adjustment) reduces the feed conversion ratio to ≤1.1 and increases feed utilization by more than 20%; it also increases the number of seedlings per unit water volume by 30%, significantly reducing aquaculture costs.
[0067] 3. Disease prevention and control are carried out in advance, reducing the amount of pesticides used.
[0068] The modular Chinese herbal medicine early warning system (immune enhancement, parasite early warning, and bacterial disease control) enables "early identification and early intervention," making the products more compliant with the requirements of green farming and pollution-free food.
[0069] 4. Intelligent operation and reduced manual input.
[0070] The machine vision-based residual bait monitoring and precision feeding system has a feeding adjustment delay of less than 30 seconds, residual bait recognition accuracy of ≥95%, and enables 24-hour unattended operation.
[0071] 5. The process is simple and easy to replicate on a large scale.
[0072] The acclimatization pond is a standard rectangular cement pond, and the supporting equipment are all commercially available products. The raw materials for water quality control, microecological preparations, and traditional Chinese medicine formulas are readily available and inexpensive, making it suitable for rapid promotion in major mandarin fish farming areas such as Central China, South China, and East China.
[0073] In summary, the large-scale breeding method for spotted mandarin fish fry based on low-salinity acclimatization and zoned feeding provided in this application provides low-salinity acclimatization ponds and releases spotted mandarin fish fry into them. Simultaneously, a zoned feeding and intelligent feed conversion system is used to transition the fry to a new diet. After the fry have completed this transition, graded water quality control and micro-ecological stability are implemented until the fry are harvested and transferred to adult fish farming. This method integrates low-salinity acclimatization, precise feeding, intelligent monitoring, and traditional Chinese medicine prevention and control. It can achieve an average total length of ≥6cm, a survival rate of ≥88%, and a feed conversion ratio of ≤1.1 for fry of the desired age. This solves the industrialization pain points of spotted mandarin fish fry, such as difficulty in transitioning to a new diet, low survival rate, and frequent disease outbreaks. It provides a complete technical solution for the large-scale, standardized, and green production of large-sized spotted mandarin fish fry, and has broad prospects for promotion and significant economic, social, and ecological benefits.
[0074] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.
Claims
1. A method for scale-up cultivation of Procyprinus procyprinus based on low-salt acclimation and partition feeding, characterized in that, The application relates to a low-salinity acclimation pond for feeding mandarin fish fry, and a feeding method thereof. The application provides a low-salinity acclimation pond; The mandarin fish fry is put into the low-salinity acclimation pond; The mandarin fish fry is fed by using a partition feeding and intelligent bait conversion system based on a light-flow compound stress feeding process; After the mandarin fish fry is fed, hierarchical water quality regulation and micro-ecological steady state construction are carried out until the mandarin fish fry is taken out of the pond and is transferred into fish culture; The water inlet of the low-salinity acclimation pond is adjusted to a salinity of 3-5‰, a hardness of 180-220 mg / L and a pH of 7.4-7.8 after precipitation, sand filtration and ultraviolet sterilization; The light-flow compound stress feeding process comprises the following steps: during the feeding of the mandarin fish fry, oxygenation is turned off at 18:00-20:00 every day, the dissolved oxygen is reduced to 4-4.5 mg / L, blue light of 200 lx is turned on, a 0.4 m / s circulation is formed in the low-salinity acclimation pond and lasts for 20 min, and a stress feeding response of the mandarin fish is induced; The low-salinity acclimation pond is provided with a water floating raft live bait area and an underwater net cage granular material area; The water floating raft live bait area accounts for 20% of the area of the low-salinity acclimation pond, and the partition feeding and intelligent bait conversion system puts live loaches or macadamia with a body length of 50-60% of the total length of the mandarin fish as live baits in the water floating raft live bait area; The underwater net cage granular material area accounts for 80% of the area of the low-salinity acclimation pond, the partition feeding and intelligent bait conversion system comprises a double-layer conical feeding net cage, the double-layer conical feeding net cage is hung in the underwater net cage granular material area, the inner layer of the double-layer conical feeding net cage is 40 meshes and the outer layer is 20 meshes, 2-4 mm slow-sinking soft granular materials are put in the double-layer conical feeding net cage, the coarse protein of the slow-sinking soft granular materials is greater than or equal to 50%, the fat is greater than or equal to 10%, and 0.4% of an attractant is contained in the slow-sinking soft granular materials.
2. The method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding according to claim 1, characterized in that, The low salinity domestication pool selects rectangular cement pool, the single pool area is 800-1200m 2 , the pool depth is 1.6-1.8m, the pool bottom is 3% slope, the slope bottom is provided with sewage collecting tank, the pool wall is provided with nano microporous oxygenation pipe and is matched with 1.5kW Roots blower; An adjustable LED light source and a waterwheel type flow creating pump are arranged on the top of the low-salinity acclimation pond to form a 0.2-0.3 m / s circulation.
3. The method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding according to claim 1, characterized in that, After the mandarin fish fry is fed, hierarchical water quality regulation and micro-ecological steady state construction are carried out until the mandarin fish fry is taken out of the pond and is transferred into fish culture; During the first 0-15 days, 1.5 mg / L of a compound micro-ecological preparation is sprinkled, 0.5 mg / L of brown sugar is supplemented the next day, the ammonia nitrogen is maintained to be less than or equal to 0.2 mg / L, and the nitrite is maintained to be less than or equal to 0.05 mg / L; During the middle period of 16-30 days, the salinity is decreased by 0.3‰ every day until the salinity in the low-salinity acclimation pond is reduced to below 0.5‰, and sodium bicarbonate is supplemented to maintain the alkalinity to be greater than or equal to 80 mg / L; During the last period of 31-45 days, the water transparency in the low-salinity acclimation pond is controlled to be between 35-40 cm, the dissolved oxygen is greater than or equal to 6 mg / L, and the temperature is 26-28 DEG C; After 45 days, the average total length of the mandarin fish fry is greater than or equal to 6 cm, the body weight is greater than or equal to 3 g, and the survival rate is greater than or equal to 88%, the mandarin fish fry is taken out of the pond and is transferred into fish culture.
4. The method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding according to claim 3, characterized in that, The compound micro-ecological preparation comprises bacillus subtilis, lactic acid bacteria and photosynthetic bacteria. wherein Bacillus subtilis is ≥ 1 x 10 10 cfu / g, lactic acid bacteria is ≥ 5 x 10 9 cfu / g, photosynthetic bacteria is ≥ 1 x 10 9 cfu / mL.
5. The method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding according to claim 1, characterized in that, The partition feeding and intelligent bait conversion system comprises an AI camera system, which comprises an underwater 4K camera, an edge computing module and a 4G wireless transmission module. The AI camera system identifies residual bait area in real time, and when the residual bait area is greater than 5%, the feeding amount is automatically reduced by 10%; when the residual bait area is less than 1%, the feeding amount is increased by 5%; wherein the feeding is performed three times a day at 07:00, 12:00 and 18:00, and the feeding amounts are 40%, 30% and 30% of the daily ration respectively, and the residual bait identification accuracy of the AI camera system is greater than or equal to 95% and the feeding adjustment delay is less than 30s; when the pellet feed intake rate is greater than or equal to 60% and lasts for 2 days, it is determined that the mandarin fish fry has completed the conversion of food, and the partition feeding and intelligent bait conversion system closes the water floating raft live bait area. The slow-sinking soft pellet comprises the following components by weight: Antarctic krill powder 30%, imported fish meal 25%, fermented soybean meal 15%, enzymatic squid paste 8%, high gluten flour 12%, fish oil 4%, lecithin 2%, compound vitamin and mineral salt 3%, mandarin fish special attractant 0.4%, and sodium alginate 0.6%. The slow-sinking soft pellet has a particle size of 2-4mm, and the slow-sinking soft pellet has a stability in water of greater than or equal to 30 minutes.
6. The method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding according to claim 1, characterized in that, During the large-scale cultivation of mandarin fish fry, a pre-warning and prevention control system is used for pre-warning and prevention control, and the pre-warning and prevention control system comprises an immune enhancement module, a parasite pre-warning module and a bacterial disease prevention control module. The immune enhancement module is configured to spray 2mg / L of a compound of astragalus polysaccharide and glycyrrhizic acid every 10 days. The parasite pre-warning module is configured to use an infrared camera to take pictures of the fish body surface from 02:00 to 04:00 every day, and when the AI identifies that the parasite attachment area is greater than or equal to 1%, 0.3mg / L of a compound of eucalyptus oil and sophoramine is sprayed. The bacterial disease prevention and control module is configured to automatically feed granules containing 2% andrographolide for 3 consecutive days when the water body vibrio concentration is ≥10 3 cfu / mL.
7. The method for large-scale breeding of spotted mandarin fish fry based on low-salt acclimatization and zoned feeding according to claim 6, characterized in that, The compound of astragalus polysaccharide and glycyrrhizic acid comprises astragalus polysaccharide, glycyrrhizic acid and a solvent. The mass ratio of the astragalus polysaccharide to the glycyrrhizic acid is 3:1, the solvent comprises physiological saline, the physiological saline accounts for 0.9% of the mass of the compound of astragalus polysaccharide and glycyrrhizic acid, and the pH of the compound of astragalus polysaccharide and glycyrrhizic acid is 6.8-7.2.
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