Composition capable of benefiting qi, nourishing blood and resisting fatigue as well as method and application of composition for preparing health-care product

Abstract

The invention belongs to the technical field of qi-tonifying, blood-nourishing and anti-fatigue health-care products, and particularly discloses a qi-tonifying, blood-nourishing and anti-fatigue composition and a method for preparing a health-care product and application thereof. Extracts of astragalus membranaceus, angelica sinensis, rhodiola rosea, corydalis saxicola and mulberries and sea cucumber peptide are added, the components are compatible according to a traditional Chinese medicine compatibility theory and refined into the extract through a modern process, and appropriate auxiliary materials are selected to prepare the capsule, so that the product can synergistically exert double effects of benefiting qi, nourishing blood and resisting fatigue. According to the invention, effective components and high-activity sea cucumber peptides in the medicinal materials are enriched by adopting a specific extraction process; the traditional Chinese medicines which are homologous in medicine and food and can be used in health-care products are processed by a modern preparation process to ensure that the product is safe and effective and can be taken for a long time; the traditional Chinese medicine composition integrates classic famous formulas of traditional Chinese medicines for benefiting qi and nourishing blood and anti-fatigue components, can play a synergistic role in benefiting qi, nourishing blood and resisting fatigue, effectively strengthens physical strength, improves deficiency of qi and blood, relieves physical fatigue, and treats both symptoms and root causes of fatigue.

Description

Technical Field

[0001] This invention relates to the technical field of health products for replenishing qi and blood and relieving fatigue, and specifically discloses a composition for replenishing qi and blood and relieving fatigue, a method for preparing health products thereon, and its application. Background Technology

[0002] In Traditional Chinese Medicine (TCM), Qi and Blood Deficiency refers to a pathological state in which both Qi and Blood deficiency coexist. In TCM theory, Qi and Blood Deficiency is closely related to fatigue, primarily manifesting as insufficient nourishment of various organs and tissues, leading to a feeling of fatigue. In today's society, with the accelerated pace of life and increased work pressure, Qi and Blood Deficiency has become a common sub-health condition among modern people. Overwork, lack of exercise, irregular eating and sleeping habits, and emotional imbalances weaken the spleen and stomach functions, resulting in insufficient Qi and Blood and physical fatigue.

[0003] Currently, the treatment of qi and blood deficiency is mainly divided into modern medical treatment and traditional Chinese medicine treatment. In modern medicine, for qi and blood deficiency caused by malnutrition, it can be treated by supplementing iron and vitamin B. 12 Nutritional supplements such as folic acid can improve anemia symptoms, thus alleviating blood and qi deficiency to some extent. Lifestyle modifications can also help. However, the benefits of these treatments are limited, and the feasibility of lifestyle changes is low. Traditional Chinese medicine (TCM) has unique advantages in treating blood and qi deficiency and fatigue, as it can regulate the body holistically. Blood and qi deficiency is a major cause of fatigue, and TCM, by replenishing qi and blood and regulating organ function, can fundamentally improve the state of blood and qi deficiency, thereby relieving fatigue.

[0004] Coptis chinensis, the dried whole herb of *Corydalis oryzae*, a plant in the Papaveraceae family, is a traditional folk medicine in southwestern China. It grows in rock crevices or along rock edges on steep cliffs at altitudes of 500-1200 meters. It possesses properties such as clearing heat and relieving pain, detoxifying and reducing inflammation, strengthening the stomach and stopping bleeding. The *Diannan Materia Medica* records that it "clears heat and reduces inflammation, and relieves sores and toxins." Modern research shows that its main components, alkaloids, steroids, and flavonoids, have hepatoprotective effects, reducing liver lipid accumulation and alleviating liver damage. It can also exert anti-tumor activity by inducing tumor cell apoptosis and has inhibitory effects on *Staphylococcus aureus*. Flavonoids can scavenge free radicals and have anti-inflammatory effects, while polysaccharides can regulate immunity. However, there are currently no research reports on the anti-fatigue effects of Coptis chinensis, and it has not yet been used in the development of health products for replenishing qi and blood and combating fatigue.

[0005] Sea cucumbers possess extremely high nutritional and medicinal value, being a high-quality food rich in protein, low in fat, and high in vitamins and minerals. Among these, sea cucumber peptides are an important marine natural product extracted from sea cucumbers. Sea cucumber peptides refer to protein hydrolysates obtained from fresh sea cucumbers through protease hydrolysis, separation, and purification, resulting in a product primarily composed of small-molecule peptides and containing multiple active ingredients. Sea cucumber peptides are rich in bioactive calcium, sea cucumber mucopolysaccharides, polypeptides, holothurin, sea cucumber saponins, amino acids, and other active components. Recent studies have shown that sea cucumber peptides play important roles in antioxidation, anti-fatigue, anti-inflammation, anti-tumor, immune regulation, and blood pressure reduction. However, the application of sea cucumber peptides in health supplements for replenishing qi and blood and combating fatigue requires further development. Summary of the Invention

[0006] In order to address the shortcomings of existing technologies and achieve the dual effects of replenishing qi and blood and relieving fatigue, this invention provides a qi-replenishing, blood-nourishing, and fatigue-relieving composition, qi-replenishing, blood-nourishing, and fatigue-relieving health product capsules, and their preparation methods and applications, using medicinal and edible herbs as the main raw materials.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] The primary objective of this invention is to provide a composition for replenishing qi and blood and relieving fatigue, comprising the following components: Astragalus membranaceus extract, Angelica sinensis extract, Rhodiola rosea extract, Coptis chinensis extract, mulberry extract, and sea cucumber peptide.

[0009] In the composition formulation of this invention, by adding extracts of Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis, and mulberry, as well as sea cucumber peptides, the composition possesses excellent dual effects of replenishing qi and blood and combating fatigue. In this combination, Astragalus membranaceus plays a role in replenishing qi and raising yang, consolidating the exterior and stopping sweating, thereby enhancing the body's vital energy. Angelica sinensis has the effects of replenishing blood and regulating menstruation, and promoting blood circulation and unblocking collaterals. Rhodiola rosea invigorates qi and blood, unblocks meridians and relieves pain, and invigorates qi and strengthens the spleen. It can rapidly improve the binding capacity of hemoglobin with oxygen, increase blood oxygen saturation, reduce the body's oxygen consumption, and increase exercise endurance, thus having anti-hypoxia and anti-fatigue effects. Coptis chinensis further enhances the anti-fatigue effect by scavenging free radicals, anti-inflammation, and regulating immunity. Mulberry nourishes yin and blood, and has antioxidant and liver-protecting effects, playing a synergistic role. Sea cucumber peptides provide rich nutrients, which can quickly provide energy to the body, accelerate the body's urea nitrogen metabolism, and increase liver glycogen reserves, thereby increasing the body's exercise endurance and anti-fatigue ability; at the same time, it improves the body's immunity and antioxidant capacity, which helps to combat fatigue. This formula combines traditional tonifying theories with modern anti-fatigue mechanisms, making it particularly suitable for treating both the symptoms and root cause of chronic fatigue syndrome. The various medicinal ingredients work synergistically to replenish qi and blood and combat fatigue.

[0010] Preferably, in the composition, the extracts of each medicinal material are in the following proportions by weight: 15-22 parts of Astragalus membranaceus extract, 8-12 parts of Angelica sinensis extract, 12-16 parts of Rhodiola rosea extract, 8-11 parts of Coptis chinensis extract, 10-15 parts of mulberry extract, and 18-25 parts of sea cucumber peptide.

[0011] By specifically selecting the mass ratio of Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis, mulberry, and sea cucumber peptide extracts, the synergistic effect of each component can be optimized, thus achieving the best dual effects of replenishing qi and blood and relieving fatigue.

[0012] Preferably, the present invention uses one or more of the following methods to extract the effective components from each medicinal material: water extraction, alcohol extraction, or ultrasound-assisted extraction.

[0013] Preferably, the conditions for the water extraction method are as follows: crush each medicinal material into powder, pass it through a 40-120 mesh sieve, add water at a volume of 8-10 times the weight of the medicinal material, extract at a temperature of 90℃-100℃, and extract for 1-2 hours.

[0014] Preferably, the conditions for the alcohol extraction method are as follows: each medicinal material is pulverized and passed through a 40-120 mesh sieve, the ethanol concentration is 60%-90%, the solvent volume (liquid-to-material ratio) is 5:1-30:1 (mL / g), the extraction temperature is 70℃-90℃, and the extraction time is 1-3 hours.

[0015] Preferably, the conditions for the ultrasonic-assisted extraction method are as follows: each medicinal material is pulverized and passed through a 40-120 mesh sieve; the ethanol concentration is 60%-90%; the solvent volume (liquid-to-material ratio) is 5:1-30:1 (mL / g); the ultrasonic frequency is 20-40kHz; and the extraction time is 30-60 minutes.

[0016] Preferably, the sea cucumber peptides are extracted using the following method:

[0017] S1. Pretreatment: Take fresh or quick-frozen sea cucumbers, remove impurities from the internal cavity, and rinse three times with deionized water; then cut the sea cucumbers into 5 mm × 5 mm cubes and expose them to UV-C shortwave radiation at 4℃, wavelength 254 nm, dose 25 J / m². 2 Irradiate at a distance of 10cm from the light source for about 15 minutes to activate endogenous matrix metalloproteinases and tissue proteases.

[0018] S2. Preparation of solid-state fermentation substrate: Pretreated sea cucumber pieces and carrier (wheat bran:soybean meal = 1:1, mass ratio) are mixed at a sea cucumber pieces:carrier ratio of 1:0.2 (mass ratio); the moisture content is adjusted to 85%-90%; simultaneously, a compound strain of Bacillus subtilis (10... 6 CFU / g matrix), Lactobacillus plantarum (10 7 (CFU / g matrix), mix well;

[0019] S3. Staged Temperature-Controlled Fermentation: The mixed substrate is loaded into the fermentation tank and controlled in two stages. Stage 1 is protein hydrolysis, dominated by Bacillus subtilis, at a temperature of 37℃±1℃, for a fermentation time of 24 h, in a micro-aerobic environment (stirring speed 50 rpm); Stage 2 is the deodorization and flavor optimization stage, dominated by Lactobacillus plantarum, at a temperature of 30℃±1℃, for a fermentation time of 24 h, in an anaerobic environment (filled with N2), with a pH ≤ 5.0.

[0020] S4. Enzymatic hydrolysate extraction and inactivation: Fermentation products were extracted with deionized water at a ratio of 1:3 (w / v) at a temperature of 50℃±2℃, with mechanical stirring at 100 rpm for 1 hour. The supernatant was separated by centrifugation at 8000 rpm for 20 minutes and then treated in a water bath at 80℃ for 10 minutes.

[0021] S5. Membrane separation and fractionation purification: The inactivated solution is filtered in stages by a series ultrafiltration system, with molecular weight cutoffs of 10 kDa (operating pressure 0.2 MPa), 3 kDa (operating pressure 0.3 MPa), 1 kDa (operating pressure 0.4 MPa), and 0.5 kDa (operating pressure 0.5 MPa) at a temperature of 25℃. The filtrate is collected in segments, retaining the components with molecular weights of 0.5-1 kDa to obtain sea cucumber peptides.

[0022] The second objective of this invention is to provide the application of the above-mentioned composition for replenishing qi and blood and relieving fatigue in the preparation of health products for replenishing qi and blood and relieving fatigue. The health products also include pharmaceutically acceptable excipients or carriers, and the dosage forms that can be made according to different needs include tablets, granules, capsules, powders, oral liquids, ointments, etc.

[0023] The third objective of this invention is to provide a health supplement capsule for replenishing qi and blood and relieving fatigue, comprising the above-mentioned composition for replenishing qi and blood and relieving fatigue, a filler, a disintegrant, and a capsule shell;

[0024] Preferably, the components of the health product capsule, by weight parts, are: 15-22 parts of Astragalus membranaceus extract, 8-12 parts of Angelica sinensis extract, 12-16 parts of Rhodiola rosea extract, 8-11 parts of Coptis chinensis extract, 10-15 parts of mulberry extract, 18-25 parts of sea cucumber peptide, 15-20 parts of filler, and 5-8 parts of disintegrant.

[0025] Preferably, the extraction methods for each medicinal herb extract and the sea cucumber peptide are the same as those described above, along with the extraction conditions.

[0026] Preferably, in terms of the selection of excipients for the capsule, a suitable filler is selected, which is one of starch, lactose, microcrystalline cellulose, and compressible starch.

[0027] Preferably, the disintegrant is one of sodium carboxymethyl starch, microcrystalline cellulose, croscarmellose sodium, or croscarmellose.

[0028] Preferably, the capsule shell is made of hydroxypropyl methylcellulose or gelatin.

[0029] By adopting the above technical solutions, the smooth production of capsules can be guaranteed. At the same time, the selection of a suitable disintegrant can make the capsules disintegrate in a timely manner, which is conducive to the release and absorption of active ingredients, thereby improving the efficacy of health products.

[0030] The fourth objective of this invention is to provide a method for preparing the above-mentioned Qi-tonifying, blood-nourishing, and fatigue-relieving health product capsules, using the following technical solution:

[0031] S1. After extracting the effective components of each medicinal material (Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis, and Morus alba) using one or more extraction methods such as water extraction, alcohol extraction, or ultrasonic-assisted extraction, the extract is concentrated under reduced pressure to a paste with a relative density of 1.10-1.35 at 60℃.

[0032] S2. The extract is dried by freeze drying or spray drying, pulverized and passed through an 80-mesh sieve. Then it is mixed evenly with sea cucumber peptides, fillers and disintegrants according to the mass ratio. An 8%-15% starch aqueous solution is used as a binder. Wet granulation is carried out to make wet granules, which are then passed through a 10-40 mesh sieve. After drying to the appropriate moisture content, the granules are sized by passing through a 12-20 mesh sieve. Finally, the granules are filled into empty capsule shells to obtain the finished capsules.

[0033] The fifth objective of this invention is to provide the application of the above-mentioned health product capsules, which have the dual effects of replenishing qi and blood and relieving fatigue.

[0034] In today's fast-paced life, people are easily troubled by sub-health, chronic fatigue syndrome, decreased immunity, cardiovascular diseases, and other problems. The Qi-nourishing and blood-tonifying anti-fatigue capsules produced by this invention can effectively alleviate physical fatigue, weakness, and low immunity caused by a fast-paced life through multiple effects such as replenishing Qi and blood, enhancing immunity, improving Qi and blood deficiency, relieving fatigue, and anti-oxidation. They can also regulate bodily functions, help people better cope with life stress, and maintain a healthy state.

[0035] Compared with the prior art, the beneficial effects of the present invention are:

[0036] (1) This invention provides a qi-tonifying, blood-nourishing, and fatigue-relieving pharmaceutical composition and a method for preparing health products therefrom. The composition includes extracts of Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis, and mulberry, as well as sea cucumber peptides. This formula is made from carefully selected classic qi-tonifying and blood-nourishing prescriptions and various anti-fatigue ingredients, through scientific proportioning and processing. It takes into account both traditional tonifying theories and modern anti-fatigue mechanisms, and is especially suitable for treating both the symptoms and root cause of chronic fatigue syndrome.

[0037] (2) This invention has found that Astragalus membranaceus and Angelica sinensis form a classic formula for replenishing Qi and nourishing blood. Astragalus membranaceus is the principal ingredient, replenishing intangible Qi to generate blood; Angelica sinensis is the assistant ingredient, transforming tangible blood to carry Qi. Mulberry harmonizes the warm and dry properties of Astragalus membranaceus and Angelica sinensis, while simultaneously nourishing Yin and blood, and providing antioxidant and liver-protecting effects, thus achieving a synergistic effect. In terms of anti-fatigue, a triple anti-fatigue combination of Rhodiola rosea, Coptis chinensis, and sea cucumber peptides is added, and Coptis chinensis has a synergistic effect in relieving fatigue. The effects of this invention are not simply the sum of the effects of individual drugs. The drugs work together to exert the dual effects of replenishing Qi and nourishing blood and relieving fatigue through multiple mechanisms such as replenishing Qi and nourishing blood, regulating immunity, promoting hematopoiesis, improving circulation, and anti-oxidation. This can meet the health and wellness needs of people in the fast-paced modern life, and is especially suitable for the prevention and improvement of Qi and blood deficiency and chronic fatigue syndrome caused by high work pressure and fast pace of life. Attached Figure Description

[0038] Figure 1 This is a statistical graph showing the changes in red blood cells (RBC), hemoglobin (HGB), and hematocrit (HCT) in the blood of mice under different treatments, compared with the normal group (blank). **** P < 0.0001; compared with Example 1 group (G1), #### P < 0.0001, ## P < 0.01, # P < 0.05. Detailed Implementation

[0039] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0040] Unless otherwise specified, the experimental methods used in the embodiments of this invention are conventional methods; unless otherwise specified, the materials and reagents used are commercially available.

[0041] The sources of commercially available medicinal herb extracts are as follows, and do not constitute a limitation on this invention: commercially available Astragalus membranaceus extract was purchased from Shanghai Yuanye Biotechnology; commercially available Angelica sinensis extract was purchased from Nuotai Biotechnology; commercially available Rhodiola rosea extract was purchased from Changqing Biotechnology; commercially available Coptis chinensis extract was purchased from Shaanxi Hana Biotechnology; commercially available mulberry extract was purchased from Shengyan Biotechnology; commercially available sea cucumber peptide was purchased from Nuotai Biotechnology; commercially available oyster peptide was purchased from Shanghai Yuanye Biotechnology; and commercially available American ginseng extract was purchased from Nuotai Biotechnology.

[0042] Example 1

[0043] A health supplement capsule for replenishing qi and blood and relieving fatigue includes the following components:

[0044] The ratio of medicinal extracts is as follows: 18 parts Astragalus membranaceus extract, 10 parts Angelica sinensis extract, 14 parts Rhodiola rosea extract, 9 parts Coptis chinensis extract, 12 parts Morus alba extract, and 20 parts sea cucumber peptide.

[0045] Excipients: 17 parts microcrystalline cellulose, 6 parts crospovidone.

[0046] The method for preparing the capsule includes the following steps:

[0047] (1) Extraction method of each medicinal material: Ultrasonic-assisted extraction was used. Each medicinal material (Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis and mulberry) was crushed and passed through an 80-mesh sieve. The ethanol concentration was 80%, the solvent volume (liquid-to-solid ratio) was 10:1 (mL / g), the ultrasonic frequency was 25kHz, and the extraction time was 60 minutes.

[0048] (2) Extraction method of sea cucumber peptides: S1, Pretreatment: Take fresh or quick-frozen sea cucumbers, remove impurities from the inner cavity and rinse with deionized water 3 times; then cut the sea cucumbers into 5 mm × 5 mm pieces, and irradiate them at 4℃ with UV-C short wave, wavelength 254 nm, dose 25 J / m², 10 cm away from the light source for about 15 minutes to activate endogenous matrix metalloproteinases and tissue proteases;

[0049] S2. Preparation of solid-state fermentation substrate: Pretreated sea cucumber pieces and carrier (wheat bran:soybean meal = 1:1, mass ratio) are mixed at a sea cucumber pieces:carrier ratio of 1:0.2 (mass ratio); the moisture content is adjusted to 85%-90%; simultaneously, a compound strain of Bacillus subtilis (10... 6 CFU / g matrix), Lactobacillus plantarum (10 7 (CFU / g matrix), mix well;

[0050] S3. Staged Temperature-Controlled Fermentation: The mixed substrate is loaded into the fermentation tank and controlled in two stages. Stage 1 is protein hydrolysis, dominated by Bacillus subtilis, at a temperature of 37℃±1℃, for a fermentation time of 24 h, in a micro-aerobic environment (stirring speed 50 rpm); Stage 2 is the deodorization and flavor optimization stage, dominated by Lactobacillus plantarum, at a temperature of 30℃±1℃, for a fermentation time of 24 h, in an anaerobic environment (filled with N2), with a pH ≤ 5.0.

[0051] S4. Enzymatic hydrolysate extraction and inactivation: Fermentation products were extracted with deionized water at a ratio of 1:3 (w / v) at a temperature of 50℃±2℃, with mechanical stirring at 100 rpm for 1 hour. The supernatant was separated by centrifugation at 8000 rpm for 20 minutes and then treated in a water bath at 80℃ for 10 minutes.

[0052] S5. Membrane separation and fractionation purification: The inactivated solution is filtered in stages by a series ultrafiltration system, with molecular weight cutoffs of 10 kDa (operating pressure 0.2 MPa), 3 kDa (operating pressure 0.3 MPa), 1 kDa (operating pressure 0.4 MPa), and 0.5 kDa (operating pressure 0.5 MPa) at a temperature of 25℃. The filtrate is collected in segments, retaining the components with molecular weights of 0.5-1 kDa to obtain sea cucumber peptides.

[0053] (3) The extracts from (1) were concentrated under reduced pressure to a relative density of 1.20 at 60°C. The extracts were then freeze-dried, pulverized, and passed through an 80-mesh sieve. The extracts were then mixed with sea cucumber peptides and excipients according to the specified ratios. A 10% starch aqueous solution was used as a binder. The mixture was wet-granulated and passed through a 40-mesh sieve. After the granules were dried to a suitable moisture content, they were granulated through a 20-mesh sieve and filled into gelatin hollow capsule shells to obtain the finished capsules.

[0054] Example 2

[0055] The difference between this embodiment and Example 1 is that the proportions of the herbal extracts in the components are as follows: 22 parts Astragalus membranaceus extract, 8 parts Angelica sinensis extract, 12 parts Rhodiola rosea extract, 11 parts Coptis chinensis extract, 10 parts Morus alba extract, and 18 parts sea cucumber peptide.

[0056] Excipients: 18 parts starch, 7 parts sodium carboxymethyl starch.

[0057] The method for preparing the capsule includes the following steps:

[0058] (1) Extraction method of each medicinal material: Water extraction was used. Each medicinal material was crushed and passed through a 60-mesh sieve. The amount of water added was 10 times the weight of the medicinal material. The extraction temperature was 95℃ and the extraction time was 1.5 hours to obtain the extract.

[0059] (2) The preparation method of sea cucumber peptide is the same as in Example 1.

[0060] (3) The extracts obtained in (1) are concentrated under reduced pressure to a paste with a relative density of 1.15 at 60°C. The paste is dried by spray drying, pulverized, and passed through an 80-mesh sieve. Then, it is mixed evenly with sea cucumber peptides and excipients according to the above ratio. A 12% starch aqueous solution is used as a binder. After wet granulation, the granules are passed through a 30-mesh sieve. After the granules are dried to a suitable moisture content, they are granulated through a 15-mesh sieve and filled into hydroxypropyl methylcellulose hollow capsule shells to obtain the finished capsules.

[0061] Example 3

[0062] The difference between this embodiment and Example 1 is that the proportions of the herbal extracts in the components are: 18 parts Astragalus membranaceus extract, 10 parts Angelica sinensis extract, and 12 parts Mulberry extract; and it does not contain sea cucumber peptides. The excipients and preparation methods are the same as in Example 1.

[0063] Example 4

[0064] The difference between this embodiment and Example 1 is that the proportions of the herbal extracts are as follows: 14 parts Rhodiola rosea extract, 9 parts Coptis chinensis extract, and 20 parts sea cucumber peptide. The excipients and preparation methods are the same as in Example 1.

[0065] Example 5

[0066] The difference between this embodiment and Example 1 is that the proportions of the herbal extracts are as follows: Rhodiola rosea extract 14 parts; sea cucumber peptide 20 parts. The excipients and preparation methods are the same as in Example 1.

[0067] Example 6

[0068] The difference between this embodiment and Example 1 is that the proportion of medicinal extracts in the components is: 9 parts of Coptis chinensis extract; and it does not contain sea cucumber peptides. The excipients and preparation methods are the same as in Example 1.

[0069] Example 7

[0070] The difference between this embodiment and Example 1 is that the proportions of the herbal extracts in the components are as follows: 18 parts Astragalus membranaceus extract, 10 parts Angelica sinensis extract, 14 parts Rhodiola rosea extract, 12 parts Mulberry extract, and 20 parts sea cucumber peptide. The excipients and preparation methods are the same as in Example 1.

[0071] Control group 1

[0072] The difference between this group and Example 1 is that the herbal extract combination and sea cucumber peptides are both commercially available products, with the following proportions: 18 parts commercially available Astragalus membranaceus extract, 10 parts commercially available Angelica sinensis extract, 14 parts commercially available Rhodiola rosea extract, 9 parts commercially available Coptis chinensis extract, 12 parts commercially available mulberry extract, and 20 parts commercially available sea cucumber peptides. The excipients and preparation methods are the same as in Example 1.

[0073] Control group 2

[0074] The difference between this group and Example 1 is that the sea cucumber peptide was replaced with 20 parts of commercially available oyster peptide. The excipients and preparation methods are the same as in Example 1.

[0075] Control group 3

[0076] The difference between this group and Example 1 is that 9 parts of commercially available American ginseng extract were added to the components. The excipients and preparation methods are the same as in Example 1.

[0077] Efficacy verification of the health products prepared by this invention

[0078] 1. Animal experiments: The weighted swimming test in mice was used to evaluate the anti-fatigue efficacy of the test product.

[0079] The "Methods for Functional Testing and Evaluation of Health Foods (2023 Edition)" states that improved exercise endurance is the most direct manifestation of enhanced anti-fatigue ability, and the length of swimming time can reflect the degree of exercise fatigue in animals. Therefore, this example uses a weighted swimming experiment in mice to evaluate the anti-fatigue effect of the capsules of this invention.

[0080] 1.1 Preparation of test substances: Drug solutions prepared in Examples 1-7 and Control Examples 1-3 were used to test the anti-fatigue effect in a weight-bearing swimming test. The capsules of the present invention were dissolved in physiological saline to a concentration of 1.0 g / ml. All drugs were administered by gavage at a dose of 0.5 ml / 10 g; the model group was given an equal volume of physiological saline. Administered once daily.

[0081] 1.2 Experimental Animals: SPF-grade Kunming mice, 6-8 weeks old, weighing 18-22 g. After acclimatization for one week, the mice were randomly divided into a blank control group (non-swimming), a model group (administered with physiological saline), a control group (administered with the control drug), and an example group (administered with the drug solution of the present invention). There were 10 mice in each of the blank control group, model group, example groups 1-7, and control groups 1-3, for a total of 120 mice.

[0082] 1.3 Experimental Instruments and Materials: Swimming tank (approximately 50cm × 50cm × 40cm), electronic balance, lead sheet, timing watch. Urea reagent kit, glycogen reagent kit, blood lactate assay kit.

[0083] The test samples were administered for 30 days. Thirty minutes after the last administration, mice carrying 5% of their body weight in lead were placed in water to swim at a temperature of 25℃±1.0℃ and a depth of at least 30cm. The lead was placed on the base of the mouse's tail at 1 / 3 to 2 / 3 of its length. A stopwatch was used to record the time from the start of swimming until the mouse's nose entered the water and remained submerged for 5-8 seconds without surfacing on its own. This was the weighted swimming time of the mouse.

[0084] 1.4 The experimental data were processed and bar charts were plotted using the statistical software GraphPad Prism 9.0.0. The results are expressed as mean ± standard deviation, n=10, and the dose is 1.0 g / ml.

[0085] Table 1. Record of Swimming Time with Weight for Mice

[0086] Note: Compared to the model group, #### P < 0.0001, # P < 0.05 ns1 This indicates no statistically significant difference; compared to the Example 1 group, **** P < 0.0001, ** P < 0.01, ns2 This indicates no statistically significant difference; compared to Group 3 of Example, ^^^^ P < 0.0001.

[0087] Results Analysis

[0088] According to the results in Table 1, compared with the model group, the weight-bearing swimming time of mice in Examples 1-7 and Control Example 1 of this invention was significantly increased, showing a significant difference. This verifies that the Qi-tonifying, blood-nourishing, and fatigue-relieving health product capsules prepared in this invention have good effects in improving physical strength and endurance and relieving fatigue.

[0089] Comparing the results of Example 1 and Control 1, it can be seen that the weight-bearing swimming time of mice in Control 1 was significantly lower than that in Example 1, indicating that the herbal extraction process used in this invention can produce components with higher activity, thus verifying the feasibility and practicality of this extraction process. Furthermore, comparing the results of Example 1 and Example 2, it can be seen that the weight-bearing swimming time of mice in Example 1 was either increased or not significantly improved compared to Example 2, indicating that the efficacy of the herbal extract obtained by adjusting the formula or other extraction methods within the scope of protection described in this invention is stable, thus verifying the effective stability of this formula within the scope of protection.

[0090] Comparing the results of Examples 1, 4-6, and the model group, it was found that *Rhizoma Rhizoma* alone in the formula had no significant anti-fatigue effect or a very weak effect. However, when used in combination with *Rhizoma Rhodiola* and sea cucumber peptides in the formula, it effectively improved the weight-bearing swimming time of mice. Furthermore, the simultaneous use of all three components significantly increased the weight-bearing swimming time of mice, indicating that *Rhizoma Rhizoma* in this formula has a synergistic anti-fatigue effect with the other two components. Meanwhile, comparing the experimental results of Examples 1 and 7, it was found that when *Rhizoma Rhizoma* was absent from the formula, the overall anti-fatigue effect was significantly reduced, indicating that *Rhizoma Rhizoma* in the formula has a synergistic effect.

[0091] Furthermore, comparing the results of groups 1, 3, and 4 with the model group, it was found that either the Qi-tonifying and blood-nourishing formula or the anti-fatigue formula alone could effectively improve the weight-bearing swimming time of mice, and the anti-fatigue formula showed a better improvement effect than the Qi-tonifying and blood-nourishing group. At the same time, the overall improvement effect of the formula was better than that of either of the two individual components, indicating a synergistic effect between the Qi-tonifying and blood-nourishing formula and the anti-fatigue formula.

[0092] Oysters are rich in protein and essential nutrients such as taurine, arginine, zinc, and selenium. Modern research shows that oyster extract, oyster peptides, can relieve fatigue, improve sexual function, enhance sperm quality, and boost immunity. American ginseng contains 16 essential trace elements, more than 18 amino acids, polysaccharides, polypeptides, and various vitamins. Modern pharmacological studies indicate that American ginseng has multiple functions, including anti-cancer, anti-fatigue, anti-hypoxia, anti-radiation, and anti-aging effects. However, comparing the results of Example 1 and Controls 2 and 3, it is clear that replacing the sea cucumber peptides in the original formula with oyster peptides, which have been shown to have significant anti-fatigue properties in modern research, or adding American ginseng extract, which has significant anti-fatigue effects, to the original formula resulted in a reduction or no significant improvement in the weight-bearing swimming time of mice. This indicates that the components of this formula are not a conventional combination, and their synergistic effect is irreplaceable.

[0093] 2. Determination of anti-fatigue biochemical indicators: The effect of the capsule of this invention in relieving physical fatigue was evaluated by detecting blood routine indicators in mice.

[0094] Measurement of serum urea (BUN), whole blood lactate (BLA), and liver glycogen:

[0095] After the above-mentioned weighted swimming, blood was collected after a 60-minute rest period. Approximately 0.5 mL of whole blood was collected after enucleation. Serum urea was measured using a urea kit, and whole blood lactate was measured using a lactate kit. The animal was immediately euthanized after blood collection from enucleation, and the liver was dissected, rinsed with physiological saline, blotted dry with filter paper, weighed, and its glycogen content was measured using a glycogen kit.

[0096] A positive result in the weighted swimming test, with any two of the three biochemical indicators—blood lactate, blood urea nitrogen, and liver glycogen—indicates that the test sample has the effect of relieving physical fatigue.

[0097] The experimental data were processed and a bar chart was plotted using the statistical software GraphPad Prism 9.0.0. The results are expressed as mean ± standard deviation, n=10, and the dose is 1.0 g / ml.

[0098] Table 2. Changes in blood lactate, blood urea nitrogen, and liver glycogen in mice after continuous drug administration.

[0099] Note: Compared with the blank control group#### P < 0.0001; compared with Example 1 group, **** P < 0.0001, ** P < 0.01, ns2 This indicates no statistically significant difference; compared to Group 3 of Example, ^^^ P < 0.001, ^^ P < 0.01, ^ P < 0.05. Compared with the model group, ns1 This indicates no statistically significant difference.

[0100] Results Analysis

[0101] Serum lactate is a product of anaerobic metabolism and reflects the degree of muscle fatigue. During strenuous exercise, glucose is anaerobicly broken down to produce lactate, which accumulates and leads to muscle soreness and weakness, a direct indicator of fatigue. Table 2 shows that serum lactate in the model group was significantly higher than that in the blank control group, indicating that weight-bearing swimming significantly leads to fatigue in mice and directly demonstrating the feasibility of the experimental conditions. Blood urea nitrogen (BUN) is a product of protein catabolism and reflects tissue damage and energy consumption. After exercise, elevated BUN results from excessive protein breakdown to provide energy and is an indicator of chronic fatigue. Liver glycogen is an energy substance stored in the liver and reflects the body's energy reserves. After exercise, liver glycogen depletion exacerbates fatigue, and low glycogen levels indicate energy depletion. Comparing the experimental results of Example 1 and Control 1, it can be seen that the product in Example 1 group has a better effect in inhibiting the increase of serum lactate and BUN than that in Control 1 group. At the same time, the product in Example 1 group has a better effect in reducing liver glycogen depletion than that in Control 1 group, and the difference is statistically significant (P < 0.01). The results of Example 1 demonstrate that it can effectively eliminate lactic acid produced during strenuous exercise, thereby alleviating fatigue. Simultaneously, Example 1 significantly reduces urea nitrogen produced during strenuous exercise, thus reducing excessive protein breakdown and improving endurance. It also increases liver glycogen reserves and reduces glycogen consumption during exercise, providing the body with more energy to combat fatigue. These results verify that the extract obtained by the herbal extraction process provided by this invention has superior activity compared to commercially available herbal extracts. Furthermore, comparing the results of Example 1 and Example 2 shows that the product of Example 1 showed no significant difference in its ability to inhibit the increase of blood lactic acid and urea nitrogen compared to Example 2, and the product of Example 1 also showed no significant difference in its ability to reduce liver glycogen consumption compared to Example 2. This indicates that the formulation of the composition of this invention has stable efficacy.

[0102] Comparing the results of Examples 1-7, it is evident that, similarly, the anti-fatigue effect of Coptis chinensis alone is extremely weak or even nonexistent, while its anti-fatigue effect is significantly enhanced when combined with Rhodiola rosea and sea cucumber peptides. Furthermore, the anti-fatigue effect is significantly weaker when Coptis chinensis is absent from the formula compared to the complete formula, indicating that Coptis chinensis has a synergistic anti-fatigue effect in this formula. Additionally, the fatigue-relieving effect of the individual anti-fatigue components is better than that of the Qi-tonifying and blood-nourishing combination formula, while the overall formula is superior to these two individual combinations, indicating a significant synergistic anti-fatigue effect between the two combinations. Moreover, comparing the results of Example 1 and Control Examples 2 and 3, it is clear that replacing the sea cucumber peptides in the original formula with oyster peptides, which have been proven by modern research to have significant anti-fatigue properties, or adding American ginseng extract, which has significant anti-fatigue effects, to the original formula does not significantly improve the anti-fatigue indicators in mice and may even worsen them. This demonstrates that this formula is not a conventional combination, and its synergistic effect cannot be easily replaced. The results of the weighted swimming experiment on mice show that this invention is not a simple combination of several medicinal materials. It verifies that there is a significant synergistic effect between Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis, sea cucumber peptide and mulberry in this invention.

[0103] In summary, this invention demonstrates that oral administration of the product solution of this invention to mice at a dose of 1.0 g / ml (equivalent to 10 times the recommended human dose) for 30 days significantly prolongs the weight-bearing swimming time of mice, significantly reduces the production of blood lactate and urea nitrogen after strenuous exercise, and significantly increases liver glycogen reserves in mice. Therefore, the Qi-tonifying, blood-nourishing, and fatigue-relieving health product capsules prepared according to this invention have the function of alleviating physical fatigue. Furthermore, the capsules prepared using the medicinal material extraction process of this invention are more effective than capsules prepared using commercially available medicinal material extracts, and the synergistic effect of Coptis chinensis extract in this formula is irreplaceable.

[0104] 3. Animal experiments: Evaluation of the hematologic efficacy of the test product by detecting hematological indicators in mice.

[0105] Drug solutions prepared in Examples 1-3 and Control Example 1 were used for the experiment. The capsules of the present invention were dissolved in physiological saline to a concentration of 1.0 g / ml. All drugs were administered by gavage at a dose of 0.5 ml / 10 g; the model group was given an equal volume of physiological saline.

[0106] Experimental animals: SPF-grade Kunming mice, 6-8 weeks old, weighing 18-22 g. Mice were randomly divided into a model group (administered with saline), a model control group (administered with the positive control drug), and an experimental group (administered with the drug solution of this invention). There were 10 mice in each of the normal group, model control group, groups 1-4 of this invention, and control group 1, for a total of 70 mice. The drug was administered once daily for 32 consecutive days. Except for the normal group, the other five groups of mice were intraperitoneally injected with cyclophosphamide 100 mg / kg on days 10-12, 18-21, and 26, 28, 30, and 31 of the drug administration, for a total of 11 times, to establish a mouse model of blood deficiency.

[0107] Experimental instruments and materials: electronic balance, blood collection equipment, blood analyzer.

[0108] Hematological parameters were measured: Blood was collected by enucleation 1 hour after the last administration (24 hours after the last intraperitoneal injection of cyclophosphamide). After anticoagulation with ethylenediaminetetraacetic acid (EDTA), the red blood cells (RBC), hemoglobin (HGB), and hematocrit (HCT) in the whole blood were automatically measured using a blood cell analyzer.

[0109] The experimental data were processed and a bar chart was plotted using the statistical software GraphPad Prism 9.0.0. The results are expressed as mean ± standard deviation, n=10, and the dose is 1.0 g / ml.

[0110] Table 3. Effects of Qi-tonifying, Blood-nourishing, and Fatigue-relieving Capsules on Hematological Indicators in Mice with Blood Deficiency Syndrome

[0111] Note: Compared to the normal group (blank), #### P < 0.0001; compared with Example 1 group (G1), **** P < 0.0001, ** P < 0.01, * P < 0.05, ns indicates no statistical difference.

[0112] According to Table 3 and Figure 1 The results showed that, compared with the normal group, the RBC, HGB, and HCT levels of the mice in the modeling control group were significantly reduced, indicating successful modeling. Meanwhile, compared with the modeling control group, the RBC, HGB, and HCT levels of mice in Examples 1-3 and Control Example 1 were significantly increased, showing significant differences and significantly improving the blood deficiency symptoms in mice, verifying that the health product capsules prepared according to this invention have good qi-tonifying and blood-nourishing effects.

[0113] Comparing the results of Example 1 (G1) and Control Example 1 (C1), it can be seen that the recovery of RBC, HGB and HCT in Example 1 was significantly higher than that in Control Example 1, indicating that the extract obtained by the specific herbal extraction process of the present invention has better efficacy than commercially available herbal extracts, thus demonstrating the practicality of the extraction process selected in the present invention.

[0114] Comparing the results of Examples 1, 3, and 4, it can be seen that compared to the complete formula in Example 1, the recovery of RBC, HGB, and HCT in Example 4, which lacks Astragalus membranaceus, Angelica sinensis, and mulberry, was significantly reduced. In contrast, the difference in Example 3, which lacks Rhodiola rosea, Coptis chinensis, and sea cucumber peptide, was not significant, indicating the necessity of Astragalus membranaceus, Angelica sinensis, and mulberry in this invention. Furthermore, combined with the results of the above-mentioned mouse weight-bearing swimming experiment, the overall qi-tonifying and blood-nourishing effects of the formulas in Examples 3 and 4 (Example 1) were superior to those of the two groups used individually, further verifying that the medicinal materials selected in this invention are not simply a combination, but rather have a synergistic effect. In addition, comparing the results of Examples 1 and 2, it can be seen that within the protection scope, adjusting the formula ratio or extraction conditions did not result in significant differences in the qi-tonifying and blood-nourishing effects between the two groups, indicating the effective stability of the composition formula of this invention.

[0115] In summary, this study demonstrates that oral administration of the solution of this invention at a dose of 1.0 g / ml (equivalent to 10 times the recommended human dose) to mice for 30 days significantly restored RBC, HGB, and HCT levels in anemic mice. Therefore, the health supplement capsules obtained in this invention have an anemia-replenishing effect.

[0116] 4. Appearance observation: Observe the appearance of the mice, such as their complexion and vitality, and record the changes in weight (statistical results are shown in Table 4), fur color, eyes, ears, tail and other physical signs of the mice in each group.

[0117] Table 4. Changes in mouse body weight after 32 days of continuous administration (mean ± standard deviation, n=10)

[0118] The results showed that there was no significant difference in body weight between the mice in each group and the normal group before drug administration (P > 0.05). After drug administration, the mice in the Example 1 group were energetic and had good motor coordination. There was no significant decrease in physical strength after modeling. During the intervention period, the body weight of the mice naturally increased, and the growth trend was not significantly different. The fur was soft and slightly shiny, with occasional slight fluffing in some areas, but no hair loss. The eyes were clear, with occasional slight dryness, but no signs of inflammation. The ear color was close to normal pink, slightly lighter than the normal group, but not significantly pale. The tail was warm and reddish, and the skin had good elasticity. The condition of the mice in the Example 2 group was similar to that of the Example 1 group, indicating that adjusting the formula ratio or extraction conditions within the protection range had no significant impact on the efficacy of the product. However, the condition of the mice in the Control Example 1 group was slightly worse than that of the Example 1 group, with signs of mild qi and blood deficiency and a lower weight gain rate (77.12%), reflecting the poor qi and blood replenishing effect of commercially available Chinese herbal extracts. The mice in Example 4 showed more severe signs of blood deficiency than those in Example 3, reflecting that the absence of the key qi-tonifying and blood-nourishing herbs Astragalus and Angelica sinensis exacerbated the qi and blood deficiency. In conclusion, this product can significantly improve the signs of blood deficiency in mice and has initially demonstrated good safety.

[0119] Obviously, the specific implementation schemes described above are merely a further detailed explanation of the purpose, technical solution and beneficial effects of the present invention. It should be understood that the above descriptions are only specific examples of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition for replenishing qi and blood and relieving fatigue, characterized in that, It includes the following components: Astragalus membranaceus extract, Angelica sinensis extract, Rhodiola rosea extract, Coptis chinensis extract, Morus alba extract, and sea cucumber peptide.

2. The composition for replenishing qi and blood and relieving fatigue according to claim 1, characterized in that, The components, by mass parts, are: Astragalus membranaceus extract 15-22 parts, Angelica sinensis extract 8-12 parts, Rhodiola rosea extract 12-16 parts, Coptis chinensis extract 8-11 parts, Morus alba extract 10-15 parts, and sea cucumber peptide 18-25 parts.

3. The composition for replenishing qi and blood and relieving fatigue according to claim 1, characterized in that, The extracts of Astragalus membranaceus, Angelica sinensis, Rhodiola rosea, Coptis chinensis, and Morus alba are extracted from the active ingredients of each medicinal material using one or more of the following methods: water extraction, alcohol extraction, or ultrasound-assisted extraction.

4. The composition for replenishing qi and blood and relieving fatigue according to claim 3, characterized in that, The conditions for the water extraction method are as follows: the raw medicinal materials are crushed and passed through a 40-120 mesh sieve, the amount of water added is 8-10 times the weight of the medicinal materials, the extraction temperature is 90℃-100℃, and the extraction time is 1-2 hours. The conditions for the alcohol extraction method are as follows: the medicinal raw materials are crushed and passed through a 40-120 mesh sieve, the ethanol concentration is 60%-90%, the solvent volume-to-solid ratio is 5:1-30:1 (mL / g), the extraction temperature is 70℃-90℃, and the extraction time is 1-3 hours. The conditions for the ultrasonic-assisted extraction method are as follows: the medicinal material is pulverized and passed through a 40-120 mesh sieve; the ethanol concentration is 60%-90%; the solvent volume-to-material mL / g ratio is 5:1-30:1; the ultrasonic frequency is 20-40kHz; and the extraction time is 30-60 minutes.

5. The composition for replenishing qi and blood and relieving fatigue according to claim 1, characterized in that, The sea cucumber peptides were extracted using the following method: S1. Pretreatment: Take fresh or quick-frozen sea cucumbers, remove impurities from the internal cavity, and rinse with water; then cut the sea cucumbers into cubes and heat them at 4℃ using UV-C shortwave at a wavelength of 254nm and a dose of 25J / m². 2 Irradiation at a distance of 10cm from the light source activates endogenous matrix metalloproteinases and cathepsins. S2. Preparation of solid-state fermentation substrate: Pretreated sea cucumber pieces and carrier are mixed at a sea cucumber piece:carrier mass ratio of 1:0.

2. The carrier is a wheat bran:soybean meal mass ratio of 1:

1. The moisture content is adjusted to 85%-90%. Simultaneously, 10 compound bacterial strains are inoculated. 6 Bacillus subtilis CFU / g matrix, 10 7 CFU / g matrix of Lactobacillus plantarum, mix well; S3. Staged temperature-controlled fermentation: The mixed substrate is loaded into the fermentation tank and controlled in two stages; Stage 1 is protein hydrolysis, dominated by Bacillus subtilis, at a temperature of 37℃±1℃, a fermentation time of 24 h, a micro-aerobic environment, and a stirring rate of 50 rpm; Stage 2 is the deodorization and flavor optimization stage, dominated by Lactobacillus plantarum, at a temperature of 30℃±1℃, a fermentation time of 24 h, anaerobic N2 filling, and pH≤5.

0. S4. Extraction and inactivation of enzymatic hydrolysate: Fermentation product was extracted with deionized water at a weight / volume ratio of 1:3 (w / v), at a temperature of 50℃±2℃, with mechanical stirring at 100 rpm for 1 hour, followed by centrifugation at 8000 rpm for 20 minutes to separate the supernatant, and then inactivated by water bath treatment at 80℃ for 10 minutes. S5. Membrane separation and fractionation purification: The inactivation solution is filtered in stages by a series ultrafiltration system, with molecular weight cutoffs of 10 kDa, 3 kDa, 1 kDa and 0.5 kDa respectively, at a temperature of 25℃. The filtrate is collected in segments, retaining the components with molecular weights of 0.5-1 kDa to obtain sea cucumber peptides.

6. The use of the composition according to any one of claims 1-5 in the preparation of a health product for replenishing qi and nourishing blood and relieving fatigue, characterized in that, The health products also include pharmaceutically acceptable excipients or carriers, and the dosage forms of the health products are tablets, granules, capsules, powders, oral liquids, and ointments.

7. A health supplement capsule for replenishing qi and blood and relieving fatigue, characterized in that, Includes the composition, filler, disintegrant, and capsule shell as described in any one of claims 1-5; Its components, by mass parts, include: 15-22 parts of Astragalus membranaceus extract, 8-12 parts of Angelica sinensis extract, 12-16 parts of Rhodiola rosea extract, 8-11 parts of Coptis chinensis extract, 10-15 parts of Morus alba extract, 18-25 parts of sea cucumber peptide, 15-20 parts of filler, and 5-8 parts of disintegrant.

8. A health supplement capsule for replenishing qi and blood and relieving fatigue according to claim 7, characterized in that, The filler is one of starch, lactose, microcrystalline cellulose, and compressible starch; the disintegrant is one of sodium carboxymethyl starch, microcrystalline cellulose, croscarmellose sodium, and croscarmellose; the capsule shell is hydroxypropyl methylcellulose or gelatin.

9. A method for preparing a Qi-tonifying, blood-nourishing, and fatigue-relieving health product capsule as described in claim 7 or 8, characterized in that, Includes the following steps: S1. Extract Astragalus membranaceus extract, Angelica sinensis extract, Rhodiola rosea extract, Coptis chinensis extract, and Morus alba extract using one or more extraction methods, including water extraction, alcohol extraction, or ultrasonic-assisted extraction. Concentrate the extract under reduced pressure to a paste with a relative density of 1.10-1.35 at 60℃. S2. After drying and pulverizing the extract, pass it through an 80-mesh sieve. Then, mix it evenly with sea cucumber peptides, fillers, and disintegrants. Use an 8%-15% starch aqueous solution as a binder and granulate it into wet granules using a wet granulation method. Then, pass the wet granules through a 10-40-mesh sieve. After drying to a suitable moisture content, granulate it through a 12-20-mesh sieve. Finally, fill the granules into empty capsule shells to obtain the finished capsules.

10. The application of the health product capsules according to claims 7 and 8, characterized in that, The application refers to the fact that the health product capsules have the dual effects of replenishing qi and blood, and relieving fatigue.

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