Algal phase regulator as well as preparation method and application thereof

Abstract

The invention relates to the technical field of aquaculture, and provides an algal phase regulator as well as a preparation method and application thereof. Active substances of yeast metabolites are compounded with composite inorganic nutrient elements, growth of beneficial algae for improving aquaculture water quality is promoted, stable algal phase and good water quality of aquaculture water are maintained, health and high efficiency of aquaculture production are guaranteed, the cost is low, and the effect is good.

Description

Technical Field

[0001] This invention relates to the field of aquaculture and water environment management technology, specifically to an algal phase regulator, its preparation method, and its application. Background Technology

[0002] Algae, as primary producers in aquatic bodies, release oxygen and convert inorganic matter into organic matter through photosynthesis, providing food for aquatic animals and maintaining the cycle of matter and energy flow. The algal community is the common name for the phytoplankton community in an aquatic ecosystem; its composition and structure directly reflect the nutrient status and environmental quality of the water body. A healthy algal community is typically characterized by high diversity, no dominant species, and strong stability, capable of providing oxygen and absorbing nutrients such as nitrogen and phosphorus through photosynthesis, thus maintaining the self-purification capacity of the water body.

[0003] A dominant green algae community produces water that is light green or yellowish-green, primarily composed of Chlorella and Scenedesmus, suitable for aquaculture and effectively purifying water quality. A dominant diatom community produces water that is brownish-red or light red, commonly seen in the early stages of eutrophication, with representative algae species such as *Nyctaginosa* and *Cladosporium*, beneficial for aquaculture. Mixed algal communities, such as a yellowish-green water body composed of diatoms and green algae, exhibit high stability and represent an ideal aquaculture environment. Superior algal communities (such as those dominated by diatoms and green algae) can increase dissolved oxygen, inhibit pathogens, and promote the growth of cultured organisms.

[0004] When cyanobacteria dominate, the water color is dark green or blue-green. Harmful algae such as Microcystis and Anabaena are dominant, easily leading to algal blooms and releasing toxins, requiring timely regulation. Harmful algal populations (such as cyanobacteria and Euglena predominantly) can cause large diurnal fluctuations in dissolved oxygen, deterioration of the bottom sediment, and even poisoning of fish and shrimp. Sudden changes in algal populations (such as water transition phenomena) are often caused by sudden weather changes or nutrient imbalances.

[0005] Excessive algal growth or outbreaks of harmful algae can lead to problems such as decreased dissolved oxygen and pH imbalance, requiring regulation to maintain algal balance. Algal regulation is a key technology for maintaining water quality balance in aquaculture, achieving ecological balance in the water body by adjusting the structure and quantity of algal populations.

[0006] Common methods for algal community control include physicochemical methods and ecological regulation. Physicochemical methods include water exchange to dilute algal concentration or the use of chlorine-based agents to kill harmful algae. Biological regulation methods include introducing probiotics to decompose organic matter, promote the reproduction of beneficial algae, and inhibit the growth of harmful algae. During the daytime, when algal photosynthesis is at its peak (usually from morning to afternoon), the effects of water exchange or chemical treatment are more significant.

[0007] Currently, existing patent document CN120058408A discloses a yeast-derived bio-organic fertilizer suitable for aquaculture, its preparation method, and its application. It comprises the following components: 40-80 parts yeast metabolites, 15-30 parts ammonium chloride, 10-20 parts monoammonium phosphate, and 3-8 parts compound microbial agent. This invention rationally combines yeast-derived organic active nutrients, effective microbial communities, and inorganic nutrients, resulting in excellent fertilization and algae cultivation effects. It can improve the aquatic environment, supplement feed organisms, promote the growth of farmed organisms, and increase product yield. Patent document CN106967655A discloses an algal phase regulator and its preparation method and application. The method involves mixing Bacillus cereus fermentation broth, Bacillus subtilis fermentation broth, Bacillus megaterium fermentation broth, Lactobacillus plantarum fermentation broth, and Candida tropicalis fermentation broth in a certain volume ratio to obtain a mixed bacterial solution. Diatomaceous earth is then added to the mixed bacterial solution as an adsorbent, stirred for adsorption, filtered through a plate and frame filter, and dried to obtain the algal phase regulator. This algal phase regulator can effectively remove cyanobacteria from cyanobacteria-cultured water, improve the aquatic environment, and promote the formation of beneficial algal phases. It has significant effects in regulating the pH value of the water, degrading ammonia nitrogen and nitrite, and regulating the algal phase, creating an excellent aquatic environment for the growth of farmed animals.

[0008] For freshwater aquaculture, the above-mentioned algal regulators all require the use of compound bacteria or their fermentation broth, which makes the cost of improving the water body relatively high. Summary of the Invention

[0009] Therefore, it is necessary to provide a method for preparing and applying an algal regulator that is low-cost and effective in improving water quality in freshwater aquaculture.

[0010] The present invention adopts the following technical solution: This invention provides an algal phase regulator for developing dominant green algae, diatoms, and mixed dominant green algae and diatoms phases in aquaculture water. It is mainly prepared by mixing yeast metabolites and a complex inorganic salt, wherein the complex inorganic salt includes at least sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate, and sodium silicate.

[0011] Preferably, for the regulation of algal community in freshwater aquaculture bodies such as ponds and reservoirs, the mass ratio of yeast metabolites to complex inorganic salts is (1~5):(1~2), and the mass ratio of sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate and sodium silicate is (15~30):(4~8):(2~4):(10~20).

[0012] Preferably, the yeast metabolites are thoroughly mixed with other inorganic salts after being treated with ultrafine grinding.

[0013] The present invention provides a method for preparing the above-mentioned algal phase regulator, comprising the following steps: obtaining yeast metabolites, ultra-fine pulverizing them to obtain yeast metabolite powder; and thoroughly mixing the yeast metabolite powder with sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate and sodium silicate to obtain the final product.

[0014] This invention provides the application of the above-mentioned algal community regulator in the development of dominant green algae, diatoms, and mixed dominant green algae and diatoms in freshwater aquaculture water. Preferably, the application method includes: for aquaculture water with a depth of less than 2m, the dosage of the algal community regulator is 8-10 kg / mu; for water depths exceeding 2m, the dosage is increased proportionally to the water volume. Preferably, after applying the algal community regulator, appropriate aeration treatment is carried out for 2-3 hours.

[0015] Compared with the prior art, the core technical advantages and beneficial effects of the present invention are as follows: This invention regulates algal communities in intensive, high-density aquaculture water bodies by combining active substances from yeast metabolites with complex inorganic nutrients. This promotes the improvement of aquaculture water quality and the growth of algae beneficial to the cultured organisms, maintains algal stability and good water quality in the aquaculture water body, provides a certain amount of live feed, ensures healthy and efficient aquaculture production, and is low in cost and effective. Attached Figure Description

[0016] Figure 1 This is a statistical chart showing the changes in algae in the sea bass farming pond before and after the use of the algae regulator in Example 1.

[0017] Figure 2 This is a statistical diagram of the algal community in the grass carp aquaculture water after 7 days of using an algal community regulator in Example 2.

[0018] Figure 3 This is a statistical diagram of the algal community in the aquaculture water after 15 days of using an algal community regulator in Example 3. Detailed Implementation

[0019] The present invention will be further described in detail below with reference to specific embodiments, so that those skilled in the art can more clearly understand the present invention. The following embodiments are only used to illustrate the present invention, and are not intended to limit the scope of the present invention. Based on the specific embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention. In the embodiments of the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art; in the embodiments of the present invention, unless specifically specified, the technical means used are conventional means well known to those skilled in the art.

[0020] Yeast metabolites: purchased from Angel Yeast Group.

[0021] Yeast metabolite powder: obtained by pulverizing using an ultrafine pulverizer.

[0022] Example 1 This embodiment provides an algal phase regulator, which is prepared by mixing yeast metabolite powder and compound inorganic nutrients in a mass ratio of 2:1. The compound inorganic nutrient formula contains 0.15g NaNO3, 0.04g MgSO4·7H2O, 0.02g K2HPO4, and 0.1g Na2SiO3·9H2O per 0.31g.

[0023] Application Case 1: In a 5-mu (approximately 0.33 hectares) intensive bass pond with a water depth of 2m and a stocking density of 1500 fish / mu, the total amount used for the entire pond is calculated at a dosage of 9kg / mu. After being fully dissolved in the aquaculture water, the solution is applied to the entire pond on a sunny morning, twice a month. Immediately after application, the aerator / water tiller is turned on for 2 hours to ensure that the algae regulator is fully dissolved in the aquaculture water. Thereafter, the aerator / water tiller is turned on for 2 hours every day to create a slow flow rate to ensure the growth and proliferation of algae.

[0024] The changes in algae before and after the use of algal regulators were detected and statistically analyzed. The results are shown below. Figure 1 .

[0025] Depend on Figure 1 The results of water color observation and fish survival activities show that after the use of algal regulator, the algal community characteristics of the predominant cyanobacteria Microcystis were significantly changed. The water color remained yellow-green, and the algae in the water were dominated by diatoms and green algae. The main dominant species were Cyclocarya diatoms and Chlorella spp., and the algal community was stable. The farmed fish fed vigorously and grew well.

[0026] Example 2 This embodiment provides an algal phase regulator, which is prepared by mixing yeast metabolite powder and composite inorganic nutrients in a mass ratio of 1:1. The composite inorganic salt formula contains 0.15g NaNO3, 0.04g MgSO4·7H2O, 0.02g K2HPO4, and 0.1g Na2SiO3·9H2O per 0.31g.

[0027] Application Case 2: The 6-mu (approximately 0.4 hectares) grass carp intensive farming pond has an average water depth of 3.0 m and a stocking density of 2,500 two-year-old grass carp per mu, 2,000 silver carp and 500 bighead carp.

[0028] Based on a dosage of 9 kg / mu, the total amount used in the whole pond is 54 kg. After fully dissolving it in the aquaculture water, it should be sprinkled throughout the pond on a sunny morning, twice a month. Immediately after application, turn on the aquaculture water machine for 2 hours to ensure that the algae regulator is fully dissolved in the aquaculture water. Thereafter, turn on the aquaculture water machine for 2 hours every day to generate a slow flow rate to ensure the growth and proliferation of algae.

[0029] The changes in phytoplankton cell density after the application of algal phase regulators were detected and statistically analyzed. The results are shown in [link to data]. Figure 2 .

[0030] Depend on Figure 2 The results of water color observation show that after the application of the algal regulator, the water color remained a light green. Chlorella, fibrous algae, and Scenedesmus, belonging to the green algae phylum, were the dominant species in the aquaculture water, with Chlorella having the highest cell density, reaching 6.1 × 10⁻⁶. 6 ind / L.

[0031] Example 3 This embodiment provides an algal phase regulator, which is prepared by mixing yeast metabolite powder and compound inorganic nutrient salts at a mass ratio of 5:1. The compound inorganic salt formula contains 0.15g NaNO3, 0.04g MgSO4·7H2O, 0.02g K2HPO4, and 0.1g Na2SiO3·9H2O per 0.31g.

[0032] Application Case 3: Silver carp and bighead carp are raised in a 45-mu pond. The fish fry are stocked at a size of 1.8-2 jin / fish, with a density of 1,000 fish / mu. 600 jin of formulated feed is fed to the fish every day.

[0033] Based on a dosage of 9 kg / mu, the total amount used in the entire pond is 405 kg. After fully dissolving it in the aquaculture water, apply it to the entire pond on a sunny morning, twice a month. Immediately after application, run the aquaculture machine for 2 hours to ensure that the algae regulator is fully dissolved in the aquaculture water. Thereafter, run the aquaculture machine for 2 hours every day to create a slow flow rate to ensure the growth and proliferation of algae.

[0034] The phytoplankton cell density was detected and statistically analyzed after the application of algal phase regulators. The results are shown in [link to data]. Figure 3 .

[0035] Depend on Figure 3 The results of water color observation show that after the algae regulator was used, the water color of the aquaculture water body was stably maintained at a light green color, and Chlorella and Chlamydomonas from the green algae phylum and Cyclophyta from the diatom phylum were the dominant species in the aquaculture water body.

[0036] It is also worth noting that, through extensive application experiments, this invention has discovered that: For algal community regulation in freshwater aquaculture bodies such as ponds and reservoirs, the preferred mass ratio of yeast metabolites to complex inorganic salts is (1~5):(1~2), and the preferred mass ratio of sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate, and sodium silicate is (15~30):(4~8):(2~4):(8~20). In the preparation method of algal community regulator, it is preferable to treat the yeast metabolites with ultrafine powder, and then mix the yeast metabolite powder with sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate, and sodium silicate thoroughly and evenly. Overall, this is beneficial to improving the water quality of aquaculture and promoting the growth of algae in aquaculture.

[0037] It should be noted that the above embodiments are only for further elaboration and explanation of the technical solution of the present invention, and are not intended to further limit the technical solution of the present invention. The method of the present invention is only a preferred embodiment and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. An algal phase regulator for developing a dominance of green algae, diatoms, or a mixture of green algae and diatoms in an aquaculture water body, characterized in that, The yeast metabolite and the complex inorganic salt are mixed to prepare the algae phase regulator, and the complex inorganic salt at least includes sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate and sodium silicate.

2. The algal phase modulator of claim 1, wherein, The mass ratio of the yeast metabolite and the complex inorganic salt is (1-5):(1-2), and the mass ratio of sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate and sodium silicate is (15-30):(4-8):(2-4):(10-20).

3. The algal phase modulator of claim 1, wherein, The yeast metabolite is subjected to ultrafine pulverization treatment.

4. The method of preparing the algal phase modulator of claim 1, characterized in that, The method comprises the following steps: The yeast metabolite is obtained, subjected to ultrafine pulverization and then obtained as yeast metabolite powder; The yeast metabolite powder is mixed with sodium nitrate, magnesium sulfate, dipotassium hydrogen phosphate and sodium silicate to obtain the algae phase regulator.

5. The use of the algae phase regulator in any one of claims 1 to 4 in developing a green algae dominant phase, a diatom dominant phase and a green algae diatom mixed dominant phase in a freshwater aquaculture water body.

6. Use according to claim 5, characterized in that, The method of the use comprises: The dosage of the algae phase regulator in the aquaculture water body with a water depth of 2 m is 8-10 kg per mu, and the dosage is increased in proportion to the water volume when the water depth is more than 2 m.

7. Use according to claim 5, characterized in that, The algae phase regulator is subjected to oxygenation treatment for 2-3 hours after application.

8. Use according to claim 5, characterized in that, The algae phase regulator is applied 1-2 times per month.

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