Torreya grandis seed oil and application thereof

Abstract

The invention discloses torreya grandis seed oil and application thereof, and belongs to the field of plant extracts. The torreya grandis seed oil is prepared by adopting a supercritical CO2 extraction method, the expression level of a macrophage RAW264.7 inflammatory factor IL-6 caused by LPS can be remarkably reduced, and the torreya grandis seed oil has a good anti-inflammatory effect. The traditional Chinese medicine composition disclosed by the invention can effectively inhibit the growth of propionibacterium acnes, also can effectively regulate the skin flora balance of people with acnes, has no obvious influence on the growth of staphylococcus epidermidis while remarkably inhibiting the growth of propionibacterium acnes, is an excellent acne-removing cosmetic raw material, and has a wide application prospect in the development of nursing products for people with acnes.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of plant extracts, in particular to a torreya grandis seed oil and its application in preventing and / or treating acne. BACKGROUND

[0002] Acne is a chronic inflammatory common skin disease, which is mostly found in adolescents, and the incidence rate is as high as more than 90%. Although the disease generally does not endanger life, it can cause patients' disfigurement or disfigurement due to its predilection for the face, and has a considerable psychological impact on patients, and can cause anxiety, depression and other psychological diseases in severe cases, so it cannot be ignored. The occurrence of acne is closely related to excessive sebum secretion, keratinization of hair follicle sebaceous duct, pathogenic bacterial infection and inflammation and other factors. Propionibacterium acnes and other follicular microorganisms are one of the main pathogenic factors of acne, which can promote inflammation in various ways to cause acne. Pathogen-associated molecular patterns on the cell wall of propionibacterium acnes, free fatty acids produced by hydrolysis of sebum and other substances can all start the skin innate immune system through stimulating TLR2 and other pathways, induce the production of inflammatory mediators and aggravate inflammatory reactions. Studies have shown that there is a bacterial flora imbalance in the skin of acne patients, the number of propionibacterium acnes increases, and the number of staphylococcus epidermidis decreases, leading to an imbalance in the ratio of bacterial flora.

[0003] At present, acne is mainly treated by using hormone antagonists, retinoid drugs, antibiotics and other drugs, but long-term use of drugs has the risk of causing allergies, kidney function damage, and even causing deformity and other toxic side effects. Selecting acne-removing cosmetic raw materials with the functions of restoring bacterial flora balance and anti-inflammatory ingredients is a safe and effective means for preventing and treating acne.

[0004] Torreya grandis is a evergreen tree of Taxaceae Torreya, which is a special economic tree species in China and rare in the world. The seed kernel of Torreya grandis has medicinal values of killing insects, reducing accumulation, moistening lung and reducing phlegm, lubricating intestines and eliminating, invigorating spleen and tonifying qi, removing blood stasis and generating new tissue. Studies have shown that Torreya grandis seed kernel extract also has physiological functions of antioxidant, prevention of atherosclerosis and anti-inflammatory. At present, Torreya grandis is often made into dried fruit food and applied in oil and health food, but rarely applied in cosmetic industry. The kernel yield of Torreya grandis is between 66.97-77.79%, the oil content is as high as 54.62-64.71%, the unsaturated fatty acid content accounts for 76.01-82.20% of the total fatty acid, and the polyunsaturated fatty acid is mainly composed of linoleic acid and oleic acid, which has multiple nutritional and health care functions such as reducing the incidence of cardiovascular diseases. In addition to unsaturated fatty acids, Torreya grandis kernel oil is also rich in various bioactive substances such as tocopherol, squalene, β-sitosterol and polyphenols, which have the effects of softening blood vessels, anti-aging, antioxidant, reducing blood lipids and improving immunity. At present, the researches on Torreya grandis seed oil mainly focus on antioxidant activity, effective reduction of the risk of suffering from cardiovascular diseases and inhibition of various plant pathogenic fungi, but there is no research on its anti-inflammatory effect and influence on normal skin flora. SUMMARY

[0005] The purpose of the present application is to provide a Torreya grandis seed oil, which can significantly reduce the expression level of inflammatory factor IL-6 of macrophage RAW264.7 caused by LPS, has good anti-inflammatory effect, can effectively regulate the skin flora balance of acne population, and significantly inhibit the growth of Propionibacterium acnes without affecting the growth of Staphylococcus epidermidis.

[0006] The specific technical solutions are as follows:

[0007] A Torreya grandis seed oil is prepared by the following steps:

[0008] After the Torreya grandis kernel is extracted by supercritical CO2, an oily extract A and a precipitate B are obtained. The precipitate B is pressed and extracted, and then centrifuged to obtain an oily extract C. The oily extract A and the oily extract C are mixed to obtain the Torreya grandis seed oil.

[0009] In some specific embodiments, the supercritical CO2 extraction step is as follows: the Torreya grandis kernel is crushed and sieved, then loaded into an extraction kettle for extraction, and the oily extract A and the precipitate B in the separation kettle are collected. The oily extract A is a light yellow translucent substance.

[0010] In some embodiments, the supercritical CO2 extraction has one or more of the following characteristics:

[0011] 1) the extraction pressure is 30-60 MPa, preferably 40 MPa;

[0012] 2) the CO2 gas flow rate is 5-10 L / h, preferably 7.5 L / h;

[0013] 3) the extraction time is 3-5 h, preferably 4 h;

[0014] 4) the extraction temperature is 30-60 °C, preferably 45-50 °C.

[0015] In some specific embodiments, the pressing extraction step is as follows: the precipitate B is loaded into a pressing device, high-temperature pressing extraction, collection of separated oil, cooling to room temperature, centrifugation to obtain supernatant oil extract C, which is a light yellow translucent oil.

[0016] In some embodiments, the pressing extraction has one or more of the following characteristics:

[0017] 1) the extraction pressure is 300-1600 Kg, preferably 750 Kg;

[0018] 2) the extraction temperature is 50-350 °C, preferably 200 °C.

[0019] In some embodiments, the centrifugation conditions are as follows: the centrifugation temperature is 0-25 °C, preferably 3 °C; the centrifugation time is 5-60 minutes, preferably 15 minutes.

[0020] The application also provides a method for preparing torreya grandis seed oil, which comprises the steps of any one of the above.

[0021] In some embodiments, the torreya grandis seed oil can be used to prepare a product for preventing and / or treating acne.

[0022] In some embodiments, the torreya grandis seed oil can be used to prepare a product with anti-inflammatory effect.

[0023] In some embodiments, the torreya grandis seed oil can be used to prepare a product for regulating the balance of skin flora.

[0024] In some embodiments, the torreya grandis seed oil can be used to prepare a product for inhibiting the growth of Propionibacterium acnes.

[0025] In some embodiments, the concentration of the torreya grandis seed oil is 0.1-15 mg / mL.

[0026] In some embodiments, the concentration of the torreya grandis seed oil is preferably 0.1-10 mg / mL.

[0027] In some specific embodiments, the concentration of the torreya grandis seed oil is further preferably 0.625-10 mg / mL.

[0028] In some embodiments, the product is a topical product selected from skin care products, cosmetic products, hair care products, or an oral product selected from solid beverages, liquid beverages, dairy products, seasoning powders, nutritional products, and oral health care products.

[0029] In some embodiments, the topical product is selected from emulsions, gels, creams, microneedle patches, and masks.

[0030] In some embodiments, the topical product further comprises an auxiliary material containing a preservative ingredient selected from one or more of methylparaben, ethylparaben, propylparaben, formaldehyde releasers, methylchloroisothiazolinone, methylisothiazolinone, phenoxyethanol, imidazolidinyl ureas, isothiazolinones, phenols, benzoic acid, benzyl alcohol, potassium sorbate, bronopol, p-hydroxyacetophenone, chlorphenesin, triclosan, quaternary ammonium salt-15, dehydroacetic acid and salts thereof.

[0031] In some embodiments, the topical product further comprises an auxiliary material containing an emulsifying ingredient selected from one or more of polyethylene glycols, glycerides, sugar derivatives, anionic emulsifiers, cationic emulsifiers, and phospholipids.

[0032] In some embodiments, the topical product further comprises an auxiliary material containing a modifying auxiliary material selected from fragrances and / or pigments.

[0033] In some embodiments, the oral product further comprises a food science acceptable auxiliary material containing a flavoring agent selected from one or more of oligofructose, lactose, steviol glycosides, xylitol, honey, sugar syrup, peppermint oil, instant tea powder, fruit juice powder, food flavoring, and citric acid.

[0034] In some embodiments, the oral product further comprises a food science acceptable auxiliary material containing a dosage form auxiliary material selected from one or more of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methylcellulose, microcrystalline cellulose, and silicon dioxide.

[0035] The present application has the following advantages:

[0036] 1) The present application uses supercritical CO2 extraction method to prepare torreya grandis seed oil, which can avoid the damage of high-temperature roasting to the effective components in the kernel during processing, and can extract more active ingredients. In addition, by high-temperature pressing extraction of the precipitate, the residual essential oil can be fully extracted, which improves the output rate of torreya grandis essential oil and reduces the waste of raw materials.

[0037] 2) The torreya grandis seed oil obtained by the application has good anti-inflammatory effect, can repair inflammation caused by acne, effectively inhibits the growth of propionibacterium acnes, and the inhibition rate of propionibacterium acnes is as high as 86.76%, has little effect on the growth of staphylococcus epidermidis, can targetly regulate the skin flora balance of acne people, and prevents and / or treats acne. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 Toxicity of torreya grandis seed oil at each concentration on macrophage RAW264.7 cells;

[0039] Figure 2 Effect of torreya grandis seed oil at a concentration of 5 mg / mL on the expression of IL-6 of macrophage RAW264.7 cells caused by LPS;

[0040] Figure 3 Effect of torreya grandis seed oil at each concentration on the proliferation of propionibacterium acnes CCSM0331. DETAILED DESCRIPTION

[0041] The application will be described in detail below in combination with the drawings and specific examples. The examples are implemented on the premise of the technical solution of the application, and detailed implementation modes and specific operation processes are given, but the protection scope of the application is not limited to the examples below.

[0042] One of the purposes of the application is to provide a torreya grandis seed oil, and the preparation steps are as follows:

[0043] After the torreya grandis kernel is extracted by supercritical CO2, an oily extract A and a precipitate B are obtained, the precipitate B is pressed and extracted, and then centrifuged to obtain an oily extract C, and then the oily extract A and the oily extract C are mixed to obtain the torreya grandis seed oil.

[0044] In some specific embodiments, the supercritical CO2 extraction step is as follows: the torreya grandis kernel is crushed and sieved, then loaded into an extraction kettle for extraction, and the oily extract A and the precipitate B in the separation kettle are collected, and the oily extract A is a light yellow translucent substance.

[0045] In some specific embodiments, the pressing extraction step is as follows: the precipitate B is loaded into a pressing device, high-temperature pressing extraction is performed, the separated oily substance is collected and cooled to room temperature, the supernatant oily extract C is obtained by centrifugation, and the oily extract C is a light yellow translucent substance.

[0046] Torreya grandis Fort. et Lindl., also known as Xiangjia, Yangjiaojia, Xiaojia, Yujia, Yesu, etc. Torreya taxifolia Am., Torreya californica Torrey and Torreya nucifera (L.) Sieb. et Zucc. are also Torreya.

[0047] In some specific embodiments, the Torreya is Torreya grandis Fort. et Lindl.

[0048] The term "supercritical CO2 extraction" is a method using supercritical carbon dioxide which has special solubility for some special natural products. The method uses the relationship between the solubility of supercritical carbon dioxide and its density, i.e. the influence of pressure and temperature on the solubility of supercritical carbon dioxide. The method can effectively prevent the oxidation and escape of heat-sensitive substances, maintain all components of medicinal plants, and extract high-boiling, low-volatility and easily pyrolyzed substances below their boiling point temperature.

[0049] In some embodiments, the extraction pressure of the supercritical CO2 extraction is 30-60 MPa.

[0050] In some specific embodiments, the extraction pressure of the supercritical CO2 extraction is preferably 40 MPa.

[0051] In some embodiments, the CO2 gas flow of the supercritical CO2 extraction is 5-10 L / h.

[0052] In some specific embodiments, the CO2 gas flow of the supercritical CO2 extraction is preferably 7.5 L / h.

[0053] In some embodiments, the extraction time of the supercritical CO2 extraction is 3-5 h, and the extraction temperature is 30-60°C.

[0054] In some specific embodiments, the extraction time of the supercritical CO2 extraction is preferably 4 h, and the extraction temperature is preferably 45-50°C.

[0055] In some embodiments, the extraction pressure of the pressing extraction is 300-1600 Kg.

[0056] In some specific embodiments, the extraction pressure of the pressing extraction is preferably 750 Kg.

[0057] Kg represents 1 Kg / cm2 = 0.1 MPa.

[0058] In some embodiments, the extraction temperature of the press extraction is 50-350℃.

[0059] In some specific embodiments, the extraction temperature of the press extraction is preferably 200℃.

[0060] In some embodiments, the centrifugation condition is a centrifugation temperature of 0-25℃, preferably 3℃; and a centrifugation time of 5-60 minutes, preferably 15 minutes.

[0061] The second object of the present application is to provide a preparation method of torreya grandis seed oil, comprising the steps of any one of the above.

[0062] The third object of the present application is to provide specific applications of the torreya grandis essential oil.

[0063] In some embodiments, the torreya grandis seed oil can be used to prepare a product for preventing and / or treating acne.

[0064] In some embodiments, the torreya grandis seed oil can be used to prepare a product with anti-inflammatory effect.

[0065] In some embodiments, the torreya grandis seed oil can be used to prepare a product for regulating skin flora balance.

[0066] In some embodiments, the torreya grandis seed oil can be used to prepare a product for inhibiting the growth of Propionibacterium acnes.

[0067] Propionibacterium acnes is a species of the genus Propionibacterium, which is the pathogenic bacteria causing acne. It is named after the production of propionic acid by fermenting glucose, and is a gram-positive bacillus, rod-shaped or slightly curved with uneven staining. It is often arranged in X, Y and V shapes, similar to the shape of pseudomembranous bacillus, and often appears as long filaments in old cultures, with high polymorphism.

[0068] In some embodiments, the concentration of the torreya grandis seed oil is 0.1-15 mg / mL.

[0069] In some embodiments, the concentration of the torreya grandis seed oil is preferably 0.1-10 mg / mL.

[0070] In some specific embodiments, the concentration of the torreya grandis seed oil is further preferably 0.625-10 mg / mL.

[0071] In some specific embodiments, the product with the above-mentioned efficacy is a topical product or an oral product.

[0072] The external product is selected from the group consisting of skin care products, cosmetic products, hair care products, preferably skin care products and / or cosmetic products;

[0073] The internal product is selected from the group consisting of solid beverages, liquid beverages, dairy products, seasoning powders, nutritional products, internal medical health products.

[0074] In some embodiments, the external product is selected from the group consisting of emulsions, gels, creams, microneedle patches, masks.

[0075] In some embodiments, the external product further contains an auxiliary material containing a preservative ingredient selected from one or more of the group consisting of methylparaben, ethylparaben, propylparaben, formaldehyde releasers, methylchloroisothiazolinone, methylisothiazolinone, phenoxyethanol, imidazolidinyl urea, isothiazolinone, phenol, benzoic acid, benzyl alcohol, potassium sorbate, bronopol, p-hydroxyacetophenone, chlorphenesin, triclosan, quaternary ammonium salt-15, dehydroacetic acid and salts thereof.

[0076] In some embodiments, the external product further contains an auxiliary material containing an emulsifying ingredient selected from one or more of the group consisting of polyethylene glycols, glycerol esters, sugar derivatives, anionic emulsifiers, cationic emulsifiers, phospholipids.

[0077] In some embodiments, the external product further contains an auxiliary material containing a modifying auxiliary material selected from the group consisting of fragrances and / or pigments.

[0078] The internal product of the present application can include all foods in the conventional sense, and can be used interchangeably with terms known in the art, such as functional foods and health functional foods.

[0079] When the torreya grandis seed oil of the present application is used as a food additive, it can be added directly or used with other foods or food ingredients, and can be appropriately used according to conventional methods.

[0080] In some embodiments, the internal product further contains a food-scientifically acceptable auxiliary material containing a flavoring agent selected from one or more of the group consisting of fructooligosaccharides, lactose, steviol glycosides, xylitol, honey, sugar syrup, peppermint oil, instant tea powder, fruit juice powder, food flavor, citric acid.

[0081] In some embodiments, the internal product further contains a food-scientifically acceptable auxiliary material containing a dosage form auxiliary material selected from one or more of the group consisting of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methylcellulose, microcrystalline cellulose, silicon dioxide.

[0082] The application will be further described in the following by way of examples without limiting the application to the examples described. The raw materials and processing techniques used in the following examples, unless otherwise specified, are all conventional commercially available raw materials and conventional processing techniques in the art.

[0083] Experimental strains:

[0084] Staphylococcus epidermidis CCSM0287 and Propionibacterium acnes CCSM0331 were isolated from human healthy facial skin.

[0085] Culture medium: Trypticase soybean agar blood culture medium (TSA): Trypticase 15 g / L, soybean papain hydrolysate 5 g / L, sodium chloride 5 g / L, agar 15 g / L, sterilized at 121℃ for 15 min, and when cooled to about 50℃, 5% sterile defibrillated sheep blood was added, mixed, and poured into plates.

[0086] Trypticase soybean broth (TSB): Trypticase 15 g / L, soybean papain hydrolysate 5 g / L, sodium chloride 5 g / L, sterilized at 121℃ for 15 min, and ready for use.

[0087] Example 1 Preparation of torreya grandis seed oil

[0088] 2 kg of torreya grandis kernel was ground through a 40 mesh sieve and loaded into an extraction kettle (HA221-50-06 type supercritical extraction device, Jiangsu Nantong Huaan Supercritical Equipment Co., Ltd.), the extraction pressure was 40 MPa, the CO2 gas flow was 7.5 L / h, the extraction time was 4 h, and the extraction temperature was 50℃. The oil-like extract A and the precipitate B in the separation kettle were collected, and the oil-like extract A was a light yellow translucent substance.

[0089] The precipitate B in the separation kettle was collected and loaded into a pressing device for high-temperature pressing extraction, the extraction pressure was 750 Kg, and the extraction temperature was 200℃. The separated oil-like substance was cooled to room temperature, centrifuged at 3℃ for 15 minutes to obtain the supernatant oil-like extract C, and the oil-like extract C was a light yellow translucent substance.

[0090] The oil-like extract A and the oil-like extract C were mixed to obtain the torreya grandis seed oil.

[0091] Example 2 Test of torreya grandis seed oil for reducing the production of inflammatory factor IL-6 by LPS-induced macrophage RAW264.7

[0092] (1) RAW264.7 cell viability experiment

[0093] RAW264.7 cells were normally cultured in high-sugar DMEM medium containing 10% fetal bovine serum under a culture condition of 5% CO2 and 37℃. When the cells grew to 80-90% confluence, they were digested with trypsin and diluted to 8×10 5The cells were seeded in 96-well plates at a density of 8 x 10

[0094] Relative cell activity % = (OD value of sample group / OD value of solvent control group) x 100%

[0095] (2) LPS-induced RAW264.7 cell IL-6 content determination experiment

[0096] The cell strain was normally cultured in a high-sugar DMEM medium containing 10% fetal bovine serum under a culture condition of 5% CO2 and 37°C. When the cells grew to 80-90% confluence, they were digested with trypsin and seeded in 96-well plates at a density of 8 x 10 5 The cells were seeded in 96-well plates at a density of 8 x 10

[0097] IL-6 relative production amount % = (IL-6 content of sample group / IL-6 content of solvent control group) x 100%

[0098] (3) Experimental results

[0099] As can be seen from Table 1, Figure 1 It can be seen that, compared with the control group, the addition amount of torreya grandis seed oil is 0.1-10 mg / mL, which does not show cytotoxicity, and can significantly promote the growth of macrophage RAW264.7 cells.

[0100] According to the results of cell viability, the concentration of 5 mg / mL of torreya grandis seed oil was selected for LPS (1 μg / mL) induced cell inflammation experiment. The experimental results show that Figure 2 ), normal cells produce a small amount of IL-6 cytokine, and after LPS treatment, cells secrete a large amount of inflammatory factor IL-6, and the addition of torreya grandis seed oil can significantly reduce the expression of inflammatory factor IL-6 (P<0.01), and the inhibition rate of torreya grandis seed oil on inflammatory factor IL-6 is 29.49%. It can be seen that torreya grandis seed oil can significantly reduce the expression of inflammatory cytokine IL-6 caused by LPS, and shows good anti-inflammatory effect.

[0101] Example 3 Influence of torreya grandis seed oil on the proliferation of Staphylococcus epidermidis CCSM0287

[0102] (1) Torreya grandis seed oil concentration: the Torreya grandis seed oil was mixed with sterile TSB medium at a ratio of 1:1 (v / v) to obtain Torreya grandis seed oil at concentrations of 0.625, 1.25, 2.5, 5, and 10 mg / mL by using the half-dilution method;

[0103] (2) Staphylococcus epidermidis CCSM0287 bacterial suspension preparation: the Staphylococcus epidermidis CCSM0287 strain milk powder freeze-dried tube stored at -80°C was streaked on a TSA solid plate and incubated at 37°C for 16-24 h to activate twice for standby; a single colony was inoculated into a 5 mL TSB liquid medium in a shaking tube, which was incubated at 37°C in a shaking incubator at a speed of 170 r / min for 16 h, and the OD 600 In the range of 0.55-0.56, the seed liquid was obtained.

[0104] (3) 96-well plates were added with the following solutions in each single well, and 4 replicate wells were prepared for each group:

[0105]

[0106] (4) The 96-well plates were incubated at 37°C for 24 h, and the OD 600 value was measured using an enzyme-labeled instrument to calculate the inhibition rate.

[0107] Inhibition rate = 1 - (OD value of the experimental group - OD value of the experimental blank group) / (OD value of the growth control well - OD value of the sterile control well) x 100%.

[0108] (5) The experimental results are shown in Table 1, and the growth inhibition rate of Torreya grandis seed oil on Staphylococcus epidermidis CCSM0287 was about 12.53%-19.37% at a concentration of 0.625-10 mg / mL, showing a certain inhibitory effect. However, the growth inhibition rate of Torreya grandis seed oil at a concentration of 10 mg / mL on Staphylococcus epidermidis CCSM0287 was less than 20%, which was lower than the intervention results of other bacteria, indicating that Torreya grandis seed oil had little effect on the skin resident bacteria Staphylococcus epidermidis.

[0109] Table 1: Inhibition rate (%) of Torreya grandis seed oil on the growth of Staphylococcus epidermidis CCSM0287

[0110]

[0111] Example 4: Effect of Torreya grandis seed oil on the proliferation of Propionibacterium acnes CCSM0331

[0112] (1) Torreya grandis seed oil concentration: the Torreya grandis seed oil was mixed with sterile TSB medium at a ratio of 1:1 (v / v) to obtain Torreya grandis seed oil at concentrations of 0.625, 1.25, 2.5, 5, and 10 mg / mL by using the half-dilution method;

[0113] (2) Preparation of P. acnes CCSM0331 bacterial suspension: Take out the P. acnes strain milk powder freeze-dried tube preserved at -80°C, streak TSA solid plate, and place in a 37°C constant temperature incubator for anaerobic culture for 48-60 h; after activation twice, the P. acnes activated for two generations is inoculated with single colonies into a test tube containing 5 mL of TSB liquid medium, and placed in a 37°C incubator for anaerobic culture for 48-60 h, and the bacterial OD value is adjusted to 0.1. 600 About 0.3, standby.

[0114] (3) Add the following solution to each single hole of a 96-hole plate, and make 4 duplicate holes for each group:

[0115]

[0116] (4) Culture the 96-hole plate at 37°C for 24 h, and measure the OD value using an enzyme marker to calculate the inhibition rate. 600

[0117] Inhibition rate = 1- (OD value of experimental group-OD value of experimental blank group) / (OD value of growth control hole-OD value of sterile control hole) x 100%

[0118] (5) Experimental results

[0119] The experimental results are shown in Table 1. Figure 3 The torreya grandis seed oil significantly inhibits P. acnes CCSM0331 (P<0.001), and the inhibition rate increases with the increase of the concentration. When the concentration is 5 mg / mL, the growth inhibition rate of P. acnes CCSM0331 is as high as 86.76%, and when the concentration is 10 mg / mL, the inhibition rate is more than 90%, indicating that the torreya grandis seed oil has significant antibacterial activity and can be used to improve acne caused by dysbacteriosis.

[0120] The above examples are only preferred embodiments of the present application, and are not any form and substantial limitation of the present application. It should be noted that for ordinary skilled persons in the art, several improvements and supplements can be made without departing from the present application, and these improvements and supplements should be considered as the protection scope of the present application.​

Claims

1. A torreya grandis seed oil, characterized by, The torreya grandis seed oil is prepared by the following steps: The torreya grandis kernel is extracted by supercritical CO2 to obtain oily extract A and precipitate B, the precipitate B is pressed and extracted, and then centrifuged to obtain oily extract C, and then the oily extract A and the oily extract C are mixed to obtain the torreya grandis seed oil.

2. The torreya grandis seed oil according to claim 1 or 2, characterized by, The supercritical CO2 extraction has one or more of the following characteristics: 1) the extraction pressure is 30-60 MPa, preferably 40 MPa; 2) the extraction CO2 gas flow is 5-10 L / h, preferably 7.5 L / h; 3) the extraction time is 3-5 h, preferably 4 h; 4) the extraction temperature is 30-60℃, preferably 45-50℃.

3. The torreya grandis seed oil according to claim 1 or 2, characterized by, The pressing extraction has one or more of the following characteristics: 1) the extraction pressure is 300-1600 Kg, preferably 750 Kg; 2) the extraction temperature is 50-350℃, preferably 200℃.

4. Toriyama seed oil according to any one of claims 1 to 3, characterized in that, The centrifugation is performed at a centrifugation temperature of 0-25℃, preferably 3℃, and a centrifugation time of 5-60 minutes, preferably 15 minutes.

5. A method for producing torreya grandis seed oil, characterized by, The method comprises the steps of any one of claims 1-5.

6. Use of the torreya grandis seed oil according to any one of claims 1-4 in the preparation of a product for: 1) preventing and / or treating acne; 2) having anti-inflammatory effect; 3) regulating the balance of skin flora; 4) inhibiting the growth of propionibacterium acnes.

7. Use according to claim 6, wherein The concentration of the torreya grandis seed oil is 0.1-15 mg / mL, preferably 0.1-10 mg / mL, and further preferably 0.625-10 mg / mL.

8. Use according to claim 6 or 7, wherein the compound is ###0002### The product is a topical product or an oral product, the topical product is selected from skin care products, cosmetics, hair care products, and the oral product is selected from solid beverages, liquid beverages, dairy products, seasoning powders, nutritional products, and oral health care products; preferably, the topical product is selected from emulsions, gels, creams, microneedle patches, and masks.

9. Use according to claim 8, wherein the compound is ###0002### The topical product further comprises an auxiliary material, and the auxiliary material has any one or more of the following characteristics: 1) contains a preservative ingredient: the preservative ingredient is selected from one or more of nipagin methyl, nipagin ethyl, nipagin propyl, formaldehyde releasers, methyl chloroisothiazolinone, methyl isothiazolinone, phenoxyethanol, imidazolidinyl urea, isothiazolinone, phenol, benzoic acid, benzyl alcohol, potassium sorbate, broopal, p-hydroxyacetophenone, chlorphenesin, triclosan, quaternary ammonium salt-15, dehydroacetic acid and its salts; 2) contains an emulsifying ingredient: the emulsifying ingredient is selected from one or more of polyethylene glycol, glycerol ester, sugar derivative, anionic emulsifier, cationic emulsifier, and phospholipid; 3) contains a modified auxiliary material: the modified ingredient is selected from essence and / or pigment.

10. The use according to claim 8, wherein the compound is ###0002### The oral product further comprises a food-scientifically acceptable auxiliary material, and the auxiliary material has any one or more of the following characteristics: 1) contains a flavoring agent, and the flavoring agent is selected from one or more of oligofructose, lactose, stevioside, xylitol, honey, syrup, peppermint oil, instant tea powder, fruit juice powder, food essence, and citric acid; 2) contains a food-scientifically acceptable auxiliary material, and the auxiliary material has any one or more of the following characteristics: 1) contains a flavoring agent, and the flavoring agent is selected from one or more of oligofructose, lactose, stevioside, xylitol, honey, syrup, peppermint oil, instant tea powder, fruit juice powder, food essence, and citric acid; 3) contains a food-scientifically acceptable auxiliary material, and the auxiliary material has any one or more of the following characteristics: 1) contains a flavoring agent, and the flavoring agent is selected from one or more of oligofructose, lactose, stevioside, xylitol, honey, syrup, peppermint oil, instant tea powder, fruit juice powder, food essence, and citric acid. 2) contains a dosage form excipient selected from one or more of the group consisting of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methylcellulose, microcrystalline cellulose, silicon dioxide. 2) contains a dosage form excipient selected from one or more of the group consisting of maltodextrin, calcium stearate, magnesium stearate, hydroxypropyl methylcellulose, microcrystalline cellulose, silicon dioxide. 2) contains a dosage form excipient selected from one or more of the group consisting of maltodextrin, calcium stearate,

Images